PRIMER_SEQUENCE_ID=NM_001790
SEQUENCE=GGTCAACGCCTGCGGCTGTTGATATTCTTGCTCAGAGGCCGTAACTTTGGCCTTCTGCTCAGGGAAGACTCTGAGTCCGACGTTGGCCTACCCAGTCGGAAGGCAGAGCTGCAATCTAGTTAACTACCTCCTTTCCCCTAGATTTCCTTTCATTCTGCTCAAGTCTTCGCCTGTGTCCGATCCCTATCTACTTTCTCTCCTCTTGTAGCAAGCCTCAGACTCCAGGCTTGAGCTAGGTTTTGTTTTTCTCCTGGTGAGAATTCGAAGACCATGTCTACGGAACTCTTCTCATCCACAAGAGAGGAAGGAAGCTCTGGCTCAGGACCCAGTTTTAGGTCTAATCAAAGGAAAATGTTAAACCTGCTCCTGGAGAGAGACACTTCCTTTACCGTCTGTCCAGATGTCCCTAGAACTCCAGTGGGCAAATTTCTTGGTGATTCTGCAAACCTAAGCATTTTGTCTGGAGGAACCCCAAAATGTTGCCTCGATCTTTCGAATCTTAGCAGTGGGGAGATAACTGCCACTCAGCTTACCACTTCTGCAGACCTTGATGAAACTGGTCACCTGGATTCTTCAGGACTTCAGGAAGTGCATTTAGCTGGGATGAATCATGACCAGCACCTAATGAAATGTAGCCCAGCACAGCTTCTTTGTAGCACTCCGAATGGTTTGGACCGTGGCCATAGAAAGAGAGATGCAATGTGTAGTTCATCTGCAAATAAAGAAAATGACAATGGAAACTTGGTGGACAGTGAAATGAAATATTTGGGCAGTCCCATTACTACTGTTCCAAAATTGGATAAAAATCCAAACCTAGGAGAAGACCAGGCAGAAGAGATTTCAGATGAATTAATGGAGTTTTCCCTGAAAGATCAAGAAGCAAAGGTGAGCAGAAGTGGCCTATATCGCTCCCCGTCGATGCCAGAGAACTTGAACAGGCCAAGACTGAAGCAGGTGGAAAAATTCAAGGACAACACAATACCAGATAAAGTTAAAAAAAAGTATTTTTCTGGCCAAGGAAAGCTCAGGAAGGGCTTATGTTTAAAGAAGACAGTCTCTCTGTGTGACATTACTATCACTCAGATGCTGGAGGAAGATTCTAACCAGGGGCACCTGATTGGTGATTTTTCCAAGGTATGTGCGCTGCCAACCGTGTCAGGGAAACACCAAGATCTGAAGTATGTCAACCCAGAAACAGTGGCTGCCTTACTGTCGGGGAAGTTCCAGGGTCTGATTGAGAAGTTTTATGTCATTGATTGTCGCTATCCATATGAGTATCTGGGAGGACACATCCAGGGAGCCTTAAACTTATATAGTCAGGAAGAACTGTTTAACTTCTTTCTGAAGAAGCCCATCGTCCCTTTGGACACCCAGAAGAGAATAATCATCGTGTTCCACTGTGAATTCTCCTCAGAGAGGGGCCCCCGAATGTGCCGCTGTCTGCGTGAAGAGGACAGGTCTCTGAACCAGTATCCTGCATTGTACTACCCAGAGCTATATATCCTTAAAGGCGGCTACAGAGACTTCTTTCCAGAATATATGGAACTGTGTGAACCACAGAGCTACTGCCCTATGCATCATCAGGACCACAAGACTGAGTTGCTGAGGTGTCGAAGCCAGAGCAAAGTGCAGGAAGGGGAGCGGCAGCTGCGGGAGCAGATTGCCCTTCTGGTGAAGGACATGAGCCCATGATAACATTCCAGCCACTGGCTGCTAACAAGTCACCAAAAAGACACTGCAGAAACCCTGAGCAGAAAGAGGCCTTCTGGATGGCCAAACCCAAGATTATTAAAAGATGTCTCTGCAAACCAACAGGCTACCAACTTGTATCCAGGCCTGGGAATGGATTAGGTTTCAGCAGAGCTGAAAGCTGGTGGCAGAGTCCTGGAGCTGGCTCTATAAGGCAGCCTTGAGTTGCATAGAGATTTGTATTGGTTCAGGGAACTCTGGCATTCCTTTTCCCAACTCCTCATGTCTTCTCACAAGCCAGCCAACTCTTTCTCTCTGGGCTTCGGGCTATGCAAGAGCGTTGTCTACCTTCTTTCTTTGTATTTTCCTTCTTTGTTTCCCCCTCTTTCTTTTTTAAAAATGGAAAAATAAACACTACAGAATGAG
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 24119, GC content failed 194, low tm 11254, high tm 6770, high any compl 4, high end compl 45, high repeat similarity 43, long poly-x seq 17, ok 5792
PRIMER_RIGHT_EXPLAIN=considered 24010, GC content failed 329, low tm 11473, high tm 6377, high any compl 1, high end compl 7, high repeat similarity 7, long poly-x seq 35, ok 5781
PRIMER_PAIR_EXPLAIN=considered 359, unacceptable product size 315, high any compl 1, high end compl 7, ok 36
PRIMER_PAIR_PENALTY=0.1246
PRIMER_LEFT_PENALTY=0.099987
PRIMER_RIGHT_PENALTY=0.024653
PRIMER_LEFT_SEQUENCE=AAAAAGTATTTTTCTGGCCAAGG
PRIMER_RIGHT_SEQUENCE=TACAAGTTGGTAGCCTGTTGGTT
PRIMER_LEFT=996,23
PRIMER_RIGHT=1828,23
PRIMER_LEFT_TM=59.900
PRIMER_RIGHT_TM=59.975
PRIMER_LEFT_GC_PERCENT=34.783
PRIMER_RIGHT_GC_PERCENT=43.478
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_MISPRIMING_SCORE=11.00, reverse MER4A a consensus
PRIMER_RIGHT_MISPRIMING_SCORE=11.00, MER21B a consensus
PRIMER_PAIR_MISPRIMING_SCORE=20.00, MER21B a consensus
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=833
PRIMER_PAIR_PENALTY_1=0.1289
PRIMER_LEFT_1_PENALTY=0.115144
PRIMER_RIGHT_1_PENALTY=0.013803
PRIMER_LEFT_1_SEQUENCE=GAAAGATCAAGAAGCAAAGGTGA
PRIMER_RIGHT_1_SEQUENCE=CTTTCCTTGGCCAGAAAAATACT
PRIMER_LEFT_1=866,23
PRIMER_RIGHT_1=1022,23
PRIMER_LEFT_1_TM=59.885
PRIMER_RIGHT_1_TM=60.014
PRIMER_LEFT_1_GC_PERCENT=39.130
PRIMER_RIGHT_1_GC_PERCENT=39.130
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_1_MISPRIMING_SCORE=11.00, L1MD2 3'-end of L1 repeat (subfamily L1MD2) - a consensus sequence
PRIMER_PAIR_1_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=5.3000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=157
PRIMER_PAIR_PENALTY_2=0.1441
PRIMER_LEFT_2_PENALTY=0.119476
PRIMER_RIGHT_2_PENALTY=0.024653
PRIMER_LEFT_2_SEQUENCE=CATCCAGGGAGCCTTAAACTTAT
PRIMER_RIGHT_2_SEQUENCE=TACAAGTTGGTAGCCTGTTGGTT
PRIMER_LEFT_2=1289,23
PRIMER_RIGHT_2=1828,23
PRIMER_LEFT_2_TM=59.881
PRIMER_RIGHT_2_TM=59.975
PRIMER_LEFT_2_GC_PERCENT=43.478
PRIMER_RIGHT_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_MISPRIMING_SCORE=11.00, reverse MER34 a consensus
PRIMER_RIGHT_2_MISPRIMING_SCORE=11.00, MER21B a consensus
PRIMER_PAIR_2_MISPRIMING_SCORE=21.00, reverse LOR1 Human low copy repetitive sequence - a consensus
PRIMER_LEFT_2_END_STABILITY=5.9000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=540
PRIMER_PAIR_PENALTY_3=0.1495
PRIMER_LEFT_3_PENALTY=0.135712
PRIMER_RIGHT_3_PENALTY=0.013803
PRIMER_LEFT_3_SEQUENCE=AAAATGACAATGGAAACTTGGTG
PRIMER_RIGHT_3_SEQUENCE=CTTTCCTTGGCCAGAAAAATACT
PRIMER_LEFT_3=724,23
PRIMER_RIGHT_3=1022,23
PRIMER_LEFT_3_TM=60.136
PRIMER_RIGHT_3_TM=60.014
PRIMER_LEFT_3_GC_PERCENT=34.783
PRIMER_RIGHT_3_GC_PERCENT=39.130
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_3_MISPRIMING_SCORE=11.00, L1MD2 3'-end of L1 repeat (subfamily L1MD2) - a consensus sequence
PRIMER_PAIR_3_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=5.3000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=299
PRIMER_PAIR_PENALTY_4=0.1592
PRIMER_LEFT_4_PENALTY=0.099987
PRIMER_RIGHT_4_PENALTY=0.059210
PRIMER_LEFT_4_SEQUENCE=AAAAAGTATTTTTCTGGCCAAGG
PRIMER_RIGHT_4_SEQUENCE=GATACAAGTTGGTAGCCTGTTGG
PRIMER_LEFT_4=996,23
PRIMER_RIGHT_4=1830,23
PRIMER_LEFT_4_TM=59.900
PRIMER_RIGHT_4_TM=59.941
PRIMER_LEFT_4_GC_PERCENT=34.783
PRIMER_RIGHT_4_GC_PERCENT=47.826
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_MISPRIMING_SCORE=11.00, reverse MER4A a consensus
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_4_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=835
PRIMER_PAIR_PENALTY_5=0.1592
PRIMER_LEFT_5_PENALTY=0.099987
PRIMER_RIGHT_5_PENALTY=0.059210
PRIMER_LEFT_5_SEQUENCE=AAAAAGTATTTTTCTGGCCAAGG
PRIMER_RIGHT_5_SEQUENCE=TGGATACAAGTTGGTAGCCTGTT
PRIMER_LEFT_5=996,23
PRIMER_RIGHT_5=1832,23
PRIMER_LEFT_5_TM=59.900
PRIMER_RIGHT_5_TM=59.941
PRIMER_LEFT_5_GC_PERCENT=34.783
PRIMER_RIGHT_5_GC_PERCENT=43.478
PRIMER_LEFT_5_SELF_ANY=8.00
PRIMER_RIGHT_5_SELF_ANY=7.00
PRIMER_LEFT_5_SELF_END=2.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_MISPRIMING_SCORE=11.00, reverse MER4A a consensus
PRIMER_RIGHT_5_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_5_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_5_END_STABILITY=8.5000
PRIMER_RIGHT_5_END_STABILITY=6.7000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_5=837
PRIMER_PAIR_PENALTY_6=0.1623
PRIMER_LEFT_6_PENALTY=0.148505
PRIMER_RIGHT_6_PENALTY=0.013803
PRIMER_LEFT_6_SEQUENCE=AGGCTTGAGCTAGGTTTTGTTTT
PRIMER_RIGHT_6_SEQUENCE=CTTTCCTTGGCCAGAAAAATACT
PRIMER_LEFT_6=223,23
PRIMER_RIGHT_6=1022,23
PRIMER_LEFT_6_TM=59.851
PRIMER_RIGHT_6_TM=60.014
PRIMER_LEFT_6_GC_PERCENT=39.130
PRIMER_RIGHT_6_GC_PERCENT=39.130
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=8.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=2.00
PRIMER_LEFT_6_MISPRIMING_SCORE=11.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_6_MISPRIMING_SCORE=11.00, L1MD2 3'-end of L1 repeat (subfamily L1MD2) - a consensus sequence
PRIMER_PAIR_6_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_6_END_STABILITY=7.0000
PRIMER_RIGHT_6_END_STABILITY=5.3000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_6=800
PRIMER_PAIR_PENALTY_7=0.1680
PRIMER_LEFT_7_PENALTY=0.143370
PRIMER_RIGHT_7_PENALTY=0.024653
PRIMER_LEFT_7_SEQUENCE=CGCTATCCATATGAGTATCTGGG
PRIMER_RIGHT_7_SEQUENCE=TACAAGTTGGTAGCCTGTTGGTT
PRIMER_LEFT_7=1260,23
PRIMER_RIGHT_7=1828,23
PRIMER_LEFT_7_TM=59.857
PRIMER_RIGHT_7_TM=59.975
PRIMER_LEFT_7_GC_PERCENT=47.826
PRIMER_RIGHT_7_GC_PERCENT=43.478
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=5.00
PRIMER_LEFT_7_SELF_END=3.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_MISPRIMING_SCORE=12.00, LTR3 LTR from human DNA related to mouse mammary tumor virus (MMTV) 3' LTR
PRIMER_RIGHT_7_MISPRIMING_SCORE=11.00, MER21B a consensus
PRIMER_PAIR_7_MISPRIMING_SCORE=21.00, LTR3 LTR from human DNA related to mouse mammary tumor virus (MMTV) 3' LTR
PRIMER_LEFT_7_END_STABILITY=9.7000
PRIMER_RIGHT_7_END_STABILITY=8.2000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_7=569
PRIMER_PAIR_PENALTY_8=0.1736
PRIMER_LEFT_8_PENALTY=0.099987
PRIMER_RIGHT_8_PENALTY=0.073619
PRIMER_LEFT_8_SEQUENCE=AAAAAGTATTTTTCTGGCCAAGG
PRIMER_RIGHT_8_SEQUENCE=AATCTCTATGCAACTCAAGGCTG
PRIMER_LEFT_8=996,23
PRIMER_RIGHT_8=1926,23
PRIMER_LEFT_8_TM=59.900
PRIMER_RIGHT_8_TM=59.926
PRIMER_LEFT_8_GC_PERCENT=34.783
PRIMER_RIGHT_8_GC_PERCENT=43.478
PRIMER_LEFT_8_SELF_ANY=8.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=2.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_MISPRIMING_SCORE=11.00, reverse MER4A a consensus
PRIMER_RIGHT_8_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_8_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_8_END_STABILITY=8.5000
PRIMER_RIGHT_8_END_STABILITY=9.7000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_8=931
PRIMER_PAIR_PENALTY_9=0.1744
PRIMER_LEFT_9_PENALTY=0.115144
PRIMER_RIGHT_9_PENALTY=0.059210
PRIMER_LEFT_9_SEQUENCE=GAAAGATCAAGAAGCAAAGGTGA
PRIMER_RIGHT_9_SEQUENCE=GATACAAGTTGGTAGCCTGTTGG
PRIMER_LEFT_9=866,23
PRIMER_RIGHT_9=1830,23
PRIMER_LEFT_9_TM=59.885
PRIMER_RIGHT_9_TM=59.941
PRIMER_LEFT_9_GC_PERCENT=39.130
PRIMER_RIGHT_9_GC_PERCENT=47.826
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=5.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_9_MISPRIMING_SCORE=24.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_9_END_STABILITY=7.9000
PRIMER_RIGHT_9_END_STABILITY=8.2000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_9=965
PRIMER_PAIR_PENALTY_10=0.1744
PRIMER_LEFT_10_PENALTY=0.115144
PRIMER_RIGHT_10_PENALTY=0.059210
PRIMER_LEFT_10_SEQUENCE=GAAAGATCAAGAAGCAAAGGTGA
PRIMER_RIGHT_10_SEQUENCE=TGGATACAAGTTGGTAGCCTGTT
PRIMER_LEFT_10=866,23
PRIMER_RIGHT_10=1832,23
PRIMER_LEFT_10_TM=59.885
PRIMER_RIGHT_10_TM=59.941
PRIMER_LEFT_10_GC_PERCENT=39.130
PRIMER_RIGHT_10_GC_PERCENT=43.478
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=7.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_10_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_10_MISPRIMING_SCORE=24.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_10_END_STABILITY=7.9000
PRIMER_RIGHT_10_END_STABILITY=6.7000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_10=967
PRIMER_PAIR_PENALTY_11=0.1787
PRIMER_LEFT_11_PENALTY=0.119476
PRIMER_RIGHT_11_PENALTY=0.059210
PRIMER_LEFT_11_SEQUENCE=CATCCAGGGAGCCTTAAACTTAT
PRIMER_RIGHT_11_SEQUENCE=GATACAAGTTGGTAGCCTGTTGG
PRIMER_LEFT_11=1289,23
PRIMER_RIGHT_11=1830,23
PRIMER_LEFT_11_TM=59.881
PRIMER_RIGHT_11_TM=59.941
PRIMER_LEFT_11_GC_PERCENT=43.478
PRIMER_RIGHT_11_GC_PERCENT=47.826
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=5.00
PRIMER_LEFT_11_SELF_END=2.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_MISPRIMING_SCORE=11.00, reverse MER34 a consensus
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_11_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_11_END_STABILITY=5.9000
PRIMER_RIGHT_11_END_STABILITY=8.2000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_11=542
PRIMER_PAIR_PENALTY_12=0.1787
PRIMER_LEFT_12_PENALTY=0.119476
PRIMER_RIGHT_12_PENALTY=0.059210
PRIMER_LEFT_12_SEQUENCE=CATCCAGGGAGCCTTAAACTTAT
PRIMER_RIGHT_12_SEQUENCE=TGGATACAAGTTGGTAGCCTGTT
PRIMER_LEFT_12=1289,23
PRIMER_RIGHT_12=1832,23
PRIMER_LEFT_12_TM=59.881
PRIMER_RIGHT_12_TM=59.941
PRIMER_LEFT_12_GC_PERCENT=43.478
PRIMER_RIGHT_12_GC_PERCENT=43.478
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=7.00
PRIMER_LEFT_12_SELF_END=2.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_MISPRIMING_SCORE=11.00, reverse MER34 a consensus
PRIMER_RIGHT_12_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_12_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_12_END_STABILITY=5.9000
PRIMER_RIGHT_12_END_STABILITY=6.7000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_12=544
PRIMER_PAIR_PENALTY_13=0.1834
PRIMER_LEFT_13_PENALTY=0.169620
PRIMER_RIGHT_13_PENALTY=0.013803
PRIMER_LEFT_13_SEQUENCE=GGCTTGAGCTAGGTTTTGTTTTT
PRIMER_RIGHT_13_SEQUENCE=CTTTCCTTGGCCAGAAAAATACT
PRIMER_LEFT_13=224,23
PRIMER_RIGHT_13=1022,23
PRIMER_LEFT_13_TM=60.170
PRIMER_RIGHT_13_TM=60.014
PRIMER_LEFT_13_GC_PERCENT=39.130
PRIMER_RIGHT_13_GC_PERCENT=39.130
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=8.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=2.00
PRIMER_LEFT_13_MISPRIMING_SCORE=11.00, MARINER1 Human mariner
PRIMER_RIGHT_13_MISPRIMING_SCORE=11.00, L1MD2 3'-end of L1 repeat (subfamily L1MD2) - a consensus sequence
PRIMER_PAIR_13_MISPRIMING_SCORE=21.00, reverse MER25 Older LINE1 5' end region - a consensus
PRIMER_LEFT_13_END_STABILITY=7.6000
PRIMER_RIGHT_13_END_STABILITY=5.3000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_13=799
PRIMER_PAIR_PENALTY_14=0.1931
PRIMER_LEFT_14_PENALTY=0.119476
PRIMER_RIGHT_14_PENALTY=0.073619
PRIMER_LEFT_14_SEQUENCE=CATCCAGGGAGCCTTAAACTTAT
PRIMER_RIGHT_14_SEQUENCE=AATCTCTATGCAACTCAAGGCTG
PRIMER_LEFT_14=1289,23
PRIMER_RIGHT_14=1926,23
PRIMER_LEFT_14_TM=59.881
PRIMER_RIGHT_14_TM=59.926
PRIMER_LEFT_14_GC_PERCENT=43.478
PRIMER_RIGHT_14_GC_PERCENT=43.478
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=2.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_MISPRIMING_SCORE=11.00, reverse MER34 a consensus
PRIMER_RIGHT_14_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_14_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_14_END_STABILITY=5.9000
PRIMER_RIGHT_14_END_STABILITY=9.7000
PRIMER_PAIR_14_COMPL_ANY=6.00
PRIMER_PAIR_14_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_14=638
PRIMER_PAIR_PENALTY_15=0.2026
PRIMER_LEFT_15_PENALTY=0.143370
PRIMER_RIGHT_15_PENALTY=0.059210
PRIMER_LEFT_15_SEQUENCE=CGCTATCCATATGAGTATCTGGG
PRIMER_RIGHT_15_SEQUENCE=GATACAAGTTGGTAGCCTGTTGG
PRIMER_LEFT_15=1260,23
PRIMER_RIGHT_15=1830,23
PRIMER_LEFT_15_TM=59.857
PRIMER_RIGHT_15_TM=59.941
PRIMER_LEFT_15_GC_PERCENT=47.826
PRIMER_RIGHT_15_GC_PERCENT=47.826
PRIMER_LEFT_15_SELF_ANY=6.00
PRIMER_RIGHT_15_SELF_ANY=5.00
PRIMER_LEFT_15_SELF_END=3.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_MISPRIMING_SCORE=12.00, LTR3 LTR from human DNA related to mouse mammary tumor virus (MMTV) 3' LTR
PRIMER_RIGHT_15_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_15_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_15_END_STABILITY=9.7000
PRIMER_RIGHT_15_END_STABILITY=8.2000
PRIMER_PAIR_15_COMPL_ANY=6.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_15=571
PRIMER_PAIR_PENALTY_16=0.2026
PRIMER_LEFT_16_PENALTY=0.143370
PRIMER_RIGHT_16_PENALTY=0.059210
PRIMER_LEFT_16_SEQUENCE=CGCTATCCATATGAGTATCTGGG
PRIMER_RIGHT_16_SEQUENCE=TGGATACAAGTTGGTAGCCTGTT
PRIMER_LEFT_16=1260,23
PRIMER_RIGHT_16=1832,23
PRIMER_LEFT_16_TM=59.857
PRIMER_RIGHT_16_TM=59.941
PRIMER_LEFT_16_GC_PERCENT=47.826
PRIMER_RIGHT_16_GC_PERCENT=43.478
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=7.00
PRIMER_LEFT_16_SELF_END=3.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_MISPRIMING_SCORE=12.00, LTR3 LTR from human DNA related to mouse mammary tumor virus (MMTV) 3' LTR
PRIMER_RIGHT_16_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_16_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_16_END_STABILITY=9.7000
PRIMER_RIGHT_16_END_STABILITY=6.7000
PRIMER_PAIR_16_COMPL_ANY=6.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_16=573
PRIMER_PAIR_PENALTY_17=0.2052
PRIMER_LEFT_17_PENALTY=0.191420
PRIMER_RIGHT_17_PENALTY=0.013803
PRIMER_LEFT_17_SEQUENCE=ACTTCTGCAGACCTTGATGAAAC
PRIMER_RIGHT_17_SEQUENCE=CTTTCCTTGGCCAGAAAAATACT
PRIMER_LEFT_17=534,23
PRIMER_RIGHT_17=1022,23
PRIMER_LEFT_17_TM=59.809
PRIMER_RIGHT_17_TM=60.014
PRIMER_LEFT_17_GC_PERCENT=43.478
PRIMER_RIGHT_17_GC_PERCENT=39.130
PRIMER_LEFT_17_SELF_ANY=8.00
PRIMER_RIGHT_17_SELF_ANY=8.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=2.00
PRIMER_LEFT_17_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_17_MISPRIMING_SCORE=11.00, L1MD2 3'-end of L1 repeat (subfamily L1MD2) - a consensus sequence
PRIMER_PAIR_17_MISPRIMING_SCORE=22.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_17_END_STABILITY=6.7000
PRIMER_RIGHT_17_END_STABILITY=5.3000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_17=489
PRIMER_PAIR_PENALTY_18=0.2061
PRIMER_LEFT_18_PENALTY=0.192311
PRIMER_RIGHT_18_PENALTY=0.013803
PRIMER_LEFT_18_SEQUENCE=TTCCCCTAGATTTCCTTTCATTC
PRIMER_RIGHT_18_SEQUENCE=CTTTCCTTGGCCAGAAAAATACT
PRIMER_LEFT_18=132,23
PRIMER_RIGHT_18=1022,23
PRIMER_LEFT_18_TM=59.808
PRIMER_RIGHT_18_TM=60.014
PRIMER_LEFT_18_GC_PERCENT=39.130
PRIMER_RIGHT_18_GC_PERCENT=39.130
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=8.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=2.00
PRIMER_LEFT_18_MISPRIMING_SCORE=11.00, MER4C a consensus
PRIMER_RIGHT_18_MISPRIMING_SCORE=11.00, L1MD2 3'-end of L1 repeat (subfamily L1MD2) - a consensus sequence
PRIMER_PAIR_18_MISPRIMING_SCORE=22.00, reverse MER25 Older LINE1 5' end region - a consensus
PRIMER_LEFT_18_END_STABILITY=6.9000
PRIMER_RIGHT_18_END_STABILITY=5.3000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_18=891
PRIMER_PAIR_PENALTY_19=0.2079
PRIMER_LEFT_19_PENALTY=0.194147
PRIMER_RIGHT_19_PENALTY=0.013803
PRIMER_LEFT_19_SEQUENCE=TGAAATGAAATATTTGGGCAGTC
PRIMER_RIGHT_19_SEQUENCE=CTTTCCTTGGCCAGAAAAATACT
PRIMER_LEFT_19=752,23
PRIMER_RIGHT_19=1022,23
PRIMER_LEFT_19_TM=60.194
PRIMER_RIGHT_19_TM=60.014
PRIMER_LEFT_19_GC_PERCENT=34.783
PRIMER_RIGHT_19_GC_PERCENT=39.130
PRIMER_LEFT_19_SELF_ANY=8.00
PRIMER_RIGHT_19_SELF_ANY=8.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=2.00
PRIMER_LEFT_19_MISPRIMING_SCORE=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_19_MISPRIMING_SCORE=11.00, L1MD2 3'-end of L1 repeat (subfamily L1MD2) - a consensus sequence
PRIMER_PAIR_19_MISPRIMING_SCORE=23.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_LEFT_19_END_STABILITY=6.4000
PRIMER_RIGHT_19_END_STABILITY=5.3000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_19=271
=