PRIMER_SEQUENCE_ID=NM_001946
SEQUENCE=GAGACGCTCGCTGTTTGTATCCATTGAGGAGCTGCCTCGCGCAGGGGGTGTGCGAGGCTGAGTCCAAGAGATAGCAAATCGAGTCTTAAATAATCCGGGGAGAAAGACGCCCGGGTAGATTTGAGGTGCAGCCTTGGAGGGAGGGATTAGAAGCCGCTAGACTTTTTTTCCTCCCCTCTCAGTAGCACGGAGTCCGAATTAATTGGATTTCATTCACTGGGGAGGAACAAAAACTATCTGGGCAGCTTCATTGAGAGAGATTCATTGACACTAAGAGCCAGCGGCTGCAGCTGGGTGCAGAGAGAACCTCCGGCTTTACTTCTGTCTCGTCTGCCCCAACCGCTAGCCTCGGCTTGGGTAAGGCGAGGCGGAATTAAACCCCGCTCCGAGAGCGGCAGCTTCGCGCGCGGTGCGCTCGGCCTATGCCTGCCCCGAGGGGCGTCTGGTAGGCACCCCGCCCTCTCCCGCAGCTCGACCCCCATGATAGATACGCTCAGACCCGTGCCCTTCGCGTCGGAAATGGCGATCAGCAAGACGGTGGCGTGGCTCAACGAGCAGCTGGAGCTGGGCAACGAGCGGCTGCTGCTGATGGACTGCCGGCCGCAGGAGCTATACGAGTCGTCGCACATCGAGTCGGCCATCAACGTGGCCATCCCGGGCATCATGCTGCGGCGCCTGCAGAAGGGTAACCTGCCGGTGCGCGCGCTCTTCACGCGCGGCGAGGACCGGGACCGCTTCACCCGGCGCTGTGGCACCGACACAGTGGTGCTCTACGACGAGAGCAGCAGCGACTGGAACGAGAATACGGGCGGCGAGTCGGTGCTCGGGCTGCTGCTCAAGAAGCTCAAGGACGAGGGCTGCCGGGCGTTCTACCTGGAAGGTGGCTTCAGTAAGTTCCAAGCCGAGTTCTCCCTGCATTGCGAGACCAATCTAGACGGCTCGTGTAGCAGCAGCTCGCCGCCGTTGCCAGTGCTGGGGCTCGGGGGCCTGCGGATCAGCTCTGACTCTTCCTCGGACATCGAGTCTGACCTTGACCGAGACCCCAATAGTGCAACAGACTCGGATGGTAGTCCGCTGTCCAACAGCCAGCCTTCCTTCCCAGTGGAGATCTTGCCCTTCCTCTACTTGGGCTGTGCCAAAGACTCCACCAACTTGGACGTGTTGGAGGAATTCGGCATCAAGTACATCTTGAACGTCACCCCCAATTTGCCGAATCTCTTTGAGAACGCAGGAGAGTTTAAATACAAGCAAATCCCCATCTCGGATCACTGGAGCCAAAACCTGTCCCAGTTTTTCCCTGAGGCCATTTCTTTCATAGATGAAGCCCGGGGCAAGAACTGTGGTGTCTTGGTACATTGCTTGGCTGGCATTAGCCGCTCAGTCACTGTGACTGTGGCTTACCTTATGCAGAAGCTCAATCTGTCGATGAACGATGCCTATGACATTGTCAAAATGAAAAAATCCAACATATCCCCTAACTTCAACTTCATGGGTCAGCTGCTGGACTTCGAGAGGACGCTGGGACTCAGCAGCCCATGTGACAACAGGGTTCCAGCACAGCAGCTGTATTTTACCACCCCTTCCAACCAGAATGTATACCAGGTGGACTCTCTGCAATCTACGTGAAAGACCCCACACCCCTCCTTGCTGGAATGTGTCTGGCCCTTCAGCAGTTTCTCTTGGCAGCATCAGCTGGGCTGCTTTCTTTGTGTGTGGCCCCAGGTGTCAAAATGACACCAGCTGTCTGTACTAGACAAGGTTACCAAGTGCGGAATTGGTTAATACTAACAGAGAGATTTGCTCCATTCTCTTTGGAATAACAGGACATGCTGTATAGATACAGGCAGTAGGTTTGCTCTGTACCCATGTGTACAGCCTACCCATGCAGGGACTGGGATTCGAGGACTTCCAGGCGCATAGGGTAGAACCAAATGATAGGGTAGGAGCATGTGTTCTTTAGGGCCTTGTAAGGCTGTTTCCTTTTGCATCTGGAACTGACTATATAATTGTCTTCAATGAAGACTAATTCAATTTTGCATATAGAGGAGCCAAAGAGAGATTTCAGCTCTGTATTTGTGGTATCAGTTTGGAAAAAAAAATCTGATACTCCATTTGATTATTGTAAATATTTGATCTTGAATCACTTGACAGTGTTTGTTTGAATTGTGTTTGTTTTTTCCTTTGATGGGCTTAAAAGAAATTATCCAAAGGGAGAAAGAGCAGTATGCCACTTCTTAAAACAGAACAAAACAAAAAAAGAAAATTGTGCTCTTTTCTAATCCAAAGGGTATATTTGCAGCATGCTTGACTTTACCAATTCTGATGACATCTTTACGGACACTATTATCACTAAGACCTTGTTATGGCGAAGTCTTTAGTCTTTTTCATGTATTTTCCTCATGATTTTTTCTCTTTATGTAGTTTGACTATGCCTTACCTTTGTAAATATTTTTGCTTGTGTTGTCGCAAAGGGGATAATCTGGGAAAGACACCAAATCATGGGCTCACTTTAAAAAAAGAAAGAATAAAAAAACCTTCAGCTGTGCTAAACAGTATATTACCTCTGTATAAAATTCTTCAGGGAGTGTCACCTCAAATGCAATACTTTGGGTTGGTTTCTTTCCTTTAAAAAAATTTGTATAAAACTGGAAGTGTGTGTGTGTGAGCATGGGTACCCATTTGATAAGAGAAATGCATTTGATTGTGAAGAAGGGAGAGTTAAATTCTCCATTATGTTCGTGGTGTAAAGTTTAGAGCTGGAATTTATTATAAGAATGTAAAACCTTAAATTATTAATAAATAACTATTTTGGCTATTGAAAAAAAAAAAAAAAAAAAAAAAA
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 31690, GC content failed 1029, low tm 12350, high tm 11691, high any compl 5, high end compl 24, high repeat similarity 28, long poly-x seq 172, ok 6391
PRIMER_RIGHT_EXPLAIN=considered 31589, GC content failed 1540, low tm 12303, high tm 11276, high any compl 2, high end compl 6, high repeat similarity 9, long poly-x seq 184, ok 6269
PRIMER_PAIR_EXPLAIN=considered 463, unacceptable product size 417, high end compl 3, ok 43
PRIMER_PAIR_PENALTY=0.0749
PRIMER_LEFT_PENALTY=0.070460
PRIMER_RIGHT_PENALTY=0.004430
PRIMER_LEFT_SEQUENCE=TATTTGCAGCATGCTTGACTTTA
PRIMER_RIGHT_SEQUENCE=CTGTTTAGCACAGCTGAAGGTTT
PRIMER_LEFT=2290,23
PRIMER_RIGHT=2552,23
PRIMER_LEFT_TM=59.930
PRIMER_RIGHT_TM=59.996
PRIMER_LEFT_GC_PERCENT=34.783
PRIMER_RIGHT_GC_PERCENT=43.478
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_MISPRIMING_SCORE=12.00, MLT2C2 Interspersed repeat MLT2C2 - a consensus
PRIMER_RIGHT_MISPRIMING_SCORE=11.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_PAIR_MISPRIMING_SCORE=22.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_END_STABILITY=6.3000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=263
PRIMER_PAIR_PENALTY_1=0.0923
PRIMER_LEFT_1_PENALTY=0.070460
PRIMER_RIGHT_1_PENALTY=0.021810
PRIMER_LEFT_1_SEQUENCE=TATTTGCAGCATGCTTGACTTTA
PRIMER_RIGHT_1_SEQUENCE=CACACACACACACTTCCAGTTTT
PRIMER_LEFT_1=2290,23
PRIMER_RIGHT_1=2663,23
PRIMER_LEFT_1_TM=59.930
PRIMER_RIGHT_1_TM=60.022
PRIMER_LEFT_1_GC_PERCENT=34.783
PRIMER_RIGHT_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_MISPRIMING_SCORE=12.00, MLT2C2 Interspersed repeat MLT2C2 - a consensus
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, reverse Tigger2 Autonomous DNA transposon
PRIMER_PAIR_1_MISPRIMING_SCORE=22.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_1_END_STABILITY=6.3000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=374
PRIMER_PAIR_PENALTY_2=0.1200
PRIMER_LEFT_2_PENALTY=0.115612
PRIMER_RIGHT_2_PENALTY=0.004430
PRIMER_LEFT_2_SEQUENCE=TTCCAACCAGAATGTATACCAGG
PRIMER_RIGHT_2_SEQUENCE=CTGTTTAGCACAGCTGAAGGTTT
PRIMER_LEFT_2=1580,23
PRIMER_RIGHT_2=2552,23
PRIMER_LEFT_2_TM=60.116
PRIMER_RIGHT_2_TM=59.996
PRIMER_LEFT_2_GC_PERCENT=43.478
PRIMER_RIGHT_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_2_MISPRIMING_SCORE=11.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_PAIR_2_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=973
PRIMER_PAIR_PENALTY_3=0.1441
PRIMER_LEFT_3_PENALTY=0.073619
PRIMER_RIGHT_3_PENALTY=0.070460
PRIMER_LEFT_3_SEQUENCE=ACCTTATGCAGAAGCTCAATCTG
PRIMER_RIGHT_3_SEQUENCE=TAAAGTCAAGCATGCTGCAAATA
PRIMER_LEFT_3=1399,23
PRIMER_RIGHT_3=2312,23
PRIMER_LEFT_3_TM=59.926
PRIMER_RIGHT_3_TM=59.930
PRIMER_LEFT_3_GC_PERCENT=43.478
PRIMER_RIGHT_3_GC_PERCENT=34.783
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, Tigger1 Autonomous DNA transposon
PRIMER_PAIR_3_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=6.2000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=914
PRIMER_PAIR_PENALTY_4=0.1647
PRIMER_LEFT_4_PENALTY=0.094274
PRIMER_RIGHT_4_PENALTY=0.070460
PRIMER_LEFT_4_SEQUENCE=AGAAGCTCAATCTGTCGATGAAC
PRIMER_RIGHT_4_SEQUENCE=TAAAGTCAAGCATGCTGCAAATA
PRIMER_LEFT_4=1408,23
PRIMER_RIGHT_4=2312,23
PRIMER_LEFT_4_TM=59.906
PRIMER_RIGHT_4_TM=59.930
PRIMER_LEFT_4_GC_PERCENT=43.478
PRIMER_RIGHT_4_GC_PERCENT=34.783
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, Tigger1 Autonomous DNA transposon
PRIMER_PAIR_4_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=905
PRIMER_PAIR_PENALTY_5=0.1822
PRIMER_LEFT_5_PENALTY=0.073619
PRIMER_RIGHT_5_PENALTY=0.108608
PRIMER_LEFT_5_SEQUENCE=CCTTATGCAGAAGCTCAATCTGT
PRIMER_RIGHT_5_SEQUENCE=CTCTTTGGCTCCTCTATATGCAA
PRIMER_LEFT_5=1400,23
PRIMER_RIGHT_5=2055,23
PRIMER_LEFT_5_TM=59.926
PRIMER_RIGHT_5_TM=59.891
PRIMER_LEFT_5_GC_PERCENT=43.478
PRIMER_RIGHT_5_GC_PERCENT=43.478
PRIMER_LEFT_5_SELF_ANY=8.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=2.00
PRIMER_LEFT_5_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_5_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_5_MISPRIMING_SCORE=21.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_5_END_STABILITY=6.4000
PRIMER_RIGHT_5_END_STABILITY=8.8000
PRIMER_PAIR_5_COMPL_ANY=6.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_5=656
PRIMER_PAIR_PENALTY_6=0.1822
PRIMER_LEFT_6_PENALTY=0.073619
PRIMER_RIGHT_6_PENALTY=0.108608
PRIMER_LEFT_6_SEQUENCE=ACCTTATGCAGAAGCTCAATCTG
PRIMER_RIGHT_6_SEQUENCE=CTCTTTGGCTCCTCTATATGCAA
PRIMER_LEFT_6=1399,23
PRIMER_RIGHT_6=2055,23
PRIMER_LEFT_6_TM=59.926
PRIMER_RIGHT_6_TM=59.891
PRIMER_LEFT_6_GC_PERCENT=43.478
PRIMER_RIGHT_6_GC_PERCENT=43.478
PRIMER_LEFT_6_SELF_ANY=8.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=2.00
PRIMER_RIGHT_6_SELF_END=2.00
PRIMER_LEFT_6_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_6_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_6_MISPRIMING_SCORE=22.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_6_END_STABILITY=6.6000
PRIMER_RIGHT_6_END_STABILITY=8.8000
PRIMER_PAIR_6_COMPL_ANY=6.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_6=657
PRIMER_PAIR_PENALTY_7=0.1861
PRIMER_LEFT_7_PENALTY=0.115612
PRIMER_RIGHT_7_PENALTY=0.070460
PRIMER_LEFT_7_SEQUENCE=TTCCAACCAGAATGTATACCAGG
PRIMER_RIGHT_7_SEQUENCE=TAAAGTCAAGCATGCTGCAAATA
PRIMER_LEFT_7=1580,23
PRIMER_RIGHT_7=2312,23
PRIMER_LEFT_7_TM=60.116
PRIMER_RIGHT_7_TM=59.930
PRIMER_LEFT_7_GC_PERCENT=43.478
PRIMER_RIGHT_7_GC_PERCENT=34.783
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=8.00
PRIMER_LEFT_7_SELF_END=3.00
PRIMER_RIGHT_7_SELF_END=2.00
PRIMER_LEFT_7_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_7_MISPRIMING_SCORE=12.00, Tigger1 Autonomous DNA transposon
PRIMER_PAIR_7_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_7_END_STABILITY=9.7000
PRIMER_RIGHT_7_END_STABILITY=6.2000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_7=733
PRIMER_PAIR_PENALTY_8=0.2029
PRIMER_LEFT_8_PENALTY=0.094274
PRIMER_RIGHT_8_PENALTY=0.108608
PRIMER_LEFT_8_SEQUENCE=AGAAGCTCAATCTGTCGATGAAC
PRIMER_RIGHT_8_SEQUENCE=CTCTTTGGCTCCTCTATATGCAA
PRIMER_LEFT_8=1408,23
PRIMER_RIGHT_8=2055,23
PRIMER_LEFT_8_TM=59.906
PRIMER_RIGHT_8_TM=59.891
PRIMER_LEFT_8_GC_PERCENT=43.478
PRIMER_RIGHT_8_GC_PERCENT=43.478
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=3.00
PRIMER_RIGHT_8_SELF_END=2.00
PRIMER_LEFT_8_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_8_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_8_END_STABILITY=6.7000
PRIMER_RIGHT_8_END_STABILITY=8.8000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_8=648
PRIMER_PAIR_PENALTY_9=0.2138
PRIMER_LEFT_9_PENALTY=0.070460
PRIMER_RIGHT_9_PENALTY=0.143370
PRIMER_LEFT_9_SEQUENCE=TATTTGCAGCATGCTTGACTTTA
PRIMER_RIGHT_9_SEQUENCE=TGCATTTCTCTTATCAAATGGGT
PRIMER_LEFT_9=2290,23
PRIMER_RIGHT_9=2695,23
PRIMER_LEFT_9_TM=59.930
PRIMER_RIGHT_9_TM=59.857
PRIMER_LEFT_9_GC_PERCENT=34.783
PRIMER_RIGHT_9_GC_PERCENT=34.783
PRIMER_LEFT_9_SELF_ANY=8.00
PRIMER_RIGHT_9_SELF_ANY=5.00
PRIMER_LEFT_9_SELF_END=2.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_MISPRIMING_SCORE=12.00, MLT2C2 Interspersed repeat MLT2C2 - a consensus
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_9_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_9_END_STABILITY=6.3000
PRIMER_RIGHT_9_END_STABILITY=9.4000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_9=406
PRIMER_PAIR_PENALTY_10=0.2165
PRIMER_LEFT_10_PENALTY=0.212114
PRIMER_RIGHT_10_PENALTY=0.004430
PRIMER_LEFT_10_SEQUENCE=GAACCAAATGATAGGGTAGGAGC
PRIMER_RIGHT_10_SEQUENCE=CTGTTTAGCACAGCTGAAGGTTT
PRIMER_LEFT_10=1924,23
PRIMER_RIGHT_10=2552,23
PRIMER_LEFT_10_TM=60.212
PRIMER_RIGHT_10_TM=59.996
PRIMER_LEFT_10_GC_PERCENT=47.826
PRIMER_RIGHT_10_GC_PERCENT=43.478
PRIMER_LEFT_10_SELF_ANY=5.00
PRIMER_RIGHT_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=2.00
PRIMER_RIGHT_10_SELF_END=2.00
PRIMER_LEFT_10_MISPRIMING_SCORE=11.00, L1MD1 3'-end of L1 repeat (subfamily L1MD1) - a consensus sequence
PRIMER_RIGHT_10_MISPRIMING_SCORE=11.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_PAIR_10_MISPRIMING_SCORE=21.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_10_END_STABILITY=9.4000
PRIMER_RIGHT_10_END_STABILITY=8.2000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_10=629
PRIMER_PAIR_PENALTY_11=0.2200
PRIMER_LEFT_11_PENALTY=0.073619
PRIMER_RIGHT_11_PENALTY=0.146403
PRIMER_LEFT_11_SEQUENCE=CCTTATGCAGAAGCTCAATCTGT
PRIMER_RIGHT_11_SEQUENCE=CAGAGCAAACCTACTGCCTGTAT
PRIMER_LEFT_11=1400,23
PRIMER_RIGHT_11=1859,23
PRIMER_LEFT_11_TM=59.926
PRIMER_RIGHT_11_TM=59.854
PRIMER_LEFT_11_GC_PERCENT=43.478
PRIMER_RIGHT_11_GC_PERCENT=47.826
PRIMER_LEFT_11_SELF_ANY=8.00
PRIMER_RIGHT_11_SELF_ANY=5.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=3.00
PRIMER_LEFT_11_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_11_MISPRIMING_SCORE=11.00, L1MD2 3'-end of L1 repeat (subfamily L1MD2) - a consensus sequence
PRIMER_PAIR_11_MISPRIMING_SCORE=21.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_11_END_STABILITY=6.4000
PRIMER_RIGHT_11_END_STABILITY=5.6000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_11=460
PRIMER_PAIR_PENALTY_12=0.2200
PRIMER_LEFT_12_PENALTY=0.073619
PRIMER_RIGHT_12_PENALTY=0.146403
PRIMER_LEFT_12_SEQUENCE=ACCTTATGCAGAAGCTCAATCTG
PRIMER_RIGHT_12_SEQUENCE=CAGAGCAAACCTACTGCCTGTAT
PRIMER_LEFT_12=1399,23
PRIMER_RIGHT_12=1859,23
PRIMER_LEFT_12_TM=59.926
PRIMER_RIGHT_12_TM=59.854
PRIMER_LEFT_12_GC_PERCENT=43.478
PRIMER_RIGHT_12_GC_PERCENT=47.826
PRIMER_LEFT_12_SELF_ANY=8.00
PRIMER_RIGHT_12_SELF_ANY=5.00
PRIMER_LEFT_12_SELF_END=2.00
PRIMER_RIGHT_12_SELF_END=3.00
PRIMER_LEFT_12_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_12_MISPRIMING_SCORE=11.00, L1MD2 3'-end of L1 repeat (subfamily L1MD2) - a consensus sequence
PRIMER_PAIR_12_MISPRIMING_SCORE=22.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_12_END_STABILITY=6.6000
PRIMER_RIGHT_12_END_STABILITY=5.6000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_12=461
PRIMER_PAIR_PENALTY_13=0.2242
PRIMER_LEFT_13_PENALTY=0.115612
PRIMER_RIGHT_13_PENALTY=0.108608
PRIMER_LEFT_13_SEQUENCE=TTCCAACCAGAATGTATACCAGG
PRIMER_RIGHT_13_SEQUENCE=CTCTTTGGCTCCTCTATATGCAA
PRIMER_LEFT_13=1580,23
PRIMER_RIGHT_13=2055,23
PRIMER_LEFT_13_TM=60.116
PRIMER_RIGHT_13_TM=59.891
PRIMER_LEFT_13_GC_PERCENT=43.478
PRIMER_RIGHT_13_GC_PERCENT=43.478
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=3.00
PRIMER_RIGHT_13_SELF_END=2.00
PRIMER_LEFT_13_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_13_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_13_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_13_END_STABILITY=9.7000
PRIMER_RIGHT_13_END_STABILITY=8.8000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_13=476
PRIMER_PAIR_PENALTY_14=0.2242
PRIMER_LEFT_14_PENALTY=0.219807
PRIMER_RIGHT_14_PENALTY=0.004430
PRIMER_LEFT_14_SEQUENCE=TGTGCTCTTTTCTAATCCAAAGG
PRIMER_RIGHT_14_SEQUENCE=CTGTTTAGCACAGCTGAAGGTTT
PRIMER_LEFT_14=2264,23
PRIMER_RIGHT_14=2552,23
PRIMER_LEFT_14_TM=59.780
PRIMER_RIGHT_14_TM=59.996
PRIMER_LEFT_14_GC_PERCENT=39.130
PRIMER_RIGHT_14_GC_PERCENT=43.478
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=3.00
PRIMER_RIGHT_14_SELF_END=2.00
PRIMER_LEFT_14_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_14_MISPRIMING_SCORE=11.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_PAIR_14_MISPRIMING_SCORE=21.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_14_END_STABILITY=8.5000
PRIMER_RIGHT_14_END_STABILITY=8.2000
PRIMER_PAIR_14_COMPL_ANY=6.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_14=289
PRIMER_PAIR_PENALTY_15=0.2292
PRIMER_LEFT_15_PENALTY=0.224760
PRIMER_RIGHT_15_PENALTY=0.004430
PRIMER_LEFT_15_SEQUENCE=TTTTGCATATAGAGGAGCCAAAG
PRIMER_RIGHT_15_SEQUENCE=CTGTTTAGCACAGCTGAAGGTTT
PRIMER_LEFT_15=2031,23
PRIMER_RIGHT_15=2552,23
PRIMER_LEFT_15_TM=59.775
PRIMER_RIGHT_15_TM=59.996
PRIMER_LEFT_15_GC_PERCENT=39.130
PRIMER_RIGHT_15_GC_PERCENT=43.478
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=2.00
PRIMER_LEFT_15_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_15_MISPRIMING_SCORE=11.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_PAIR_15_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_15_END_STABILITY=7.3000
PRIMER_RIGHT_15_END_STABILITY=8.2000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_15=522
PRIMER_PAIR_PENALTY_16=0.2339
PRIMER_LEFT_16_PENALTY=0.212114
PRIMER_RIGHT_16_PENALTY=0.021810
PRIMER_LEFT_16_SEQUENCE=GAACCAAATGATAGGGTAGGAGC
PRIMER_RIGHT_16_SEQUENCE=CACACACACACACTTCCAGTTTT
PRIMER_LEFT_16=1924,23
PRIMER_RIGHT_16=2663,23
PRIMER_LEFT_16_TM=60.212
PRIMER_RIGHT_16_TM=60.022
PRIMER_LEFT_16_GC_PERCENT=47.826
PRIMER_RIGHT_16_GC_PERCENT=43.478
PRIMER_LEFT_16_SELF_ANY=5.00
PRIMER_RIGHT_16_SELF_ANY=3.00
PRIMER_LEFT_16_SELF_END=2.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_MISPRIMING_SCORE=11.00, L1MD1 3'-end of L1 repeat (subfamily L1MD1) - a consensus sequence
PRIMER_RIGHT_16_MISPRIMING_SCORE=12.00, reverse Tigger2 Autonomous DNA transposon
PRIMER_PAIR_16_MISPRIMING_SCORE=21.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_16_END_STABILITY=9.4000
PRIMER_RIGHT_16_END_STABILITY=7.0000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_16=740
PRIMER_PAIR_PENALTY_17=0.2351
PRIMER_LEFT_17_PENALTY=0.070460
PRIMER_RIGHT_17_PENALTY=0.164660
PRIMER_LEFT_17_SEQUENCE=TATTTGCAGCATGCTTGACTTTA
PRIMER_RIGHT_17_SEQUENCE=CTCACACACACACACTTCCAGTT
PRIMER_LEFT_17=2290,23
PRIMER_RIGHT_17=2665,23
PRIMER_LEFT_17_TM=59.930
PRIMER_RIGHT_17_TM=60.165
PRIMER_LEFT_17_GC_PERCENT=34.783
PRIMER_RIGHT_17_GC_PERCENT=47.826
PRIMER_LEFT_17_SELF_ANY=8.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=2.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_MISPRIMING_SCORE=12.00, MLT2C2 Interspersed repeat MLT2C2 - a consensus
PRIMER_RIGHT_17_MISPRIMING_SCORE=12.00, MER6 Nonautonomous DNA transposon
PRIMER_PAIR_17_MISPRIMING_SCORE=23.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_17_END_STABILITY=6.3000
PRIMER_RIGHT_17_END_STABILITY=6.7000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_17=376
PRIMER_PAIR_PENALTY_18=0.2407
PRIMER_LEFT_18_PENALTY=0.094274
PRIMER_RIGHT_18_PENALTY=0.146403
PRIMER_LEFT_18_SEQUENCE=AGAAGCTCAATCTGTCGATGAAC
PRIMER_RIGHT_18_SEQUENCE=CAGAGCAAACCTACTGCCTGTAT
PRIMER_LEFT_18=1408,23
PRIMER_RIGHT_18=1859,23
PRIMER_LEFT_18_TM=59.906
PRIMER_RIGHT_18_TM=59.854
PRIMER_LEFT_18_GC_PERCENT=43.478
PRIMER_RIGHT_18_GC_PERCENT=47.826
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=5.00
PRIMER_LEFT_18_SELF_END=3.00
PRIMER_RIGHT_18_SELF_END=3.00
PRIMER_LEFT_18_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_18_MISPRIMING_SCORE=11.00, L1MD2 3'-end of L1 repeat (subfamily L1MD2) - a consensus sequence
PRIMER_PAIR_18_MISPRIMING_SCORE=21.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_18_END_STABILITY=6.7000
PRIMER_RIGHT_18_END_STABILITY=5.6000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_18=452
PRIMER_PAIR_PENALTY_19=0.2416
PRIMER_LEFT_19_PENALTY=0.219807
PRIMER_RIGHT_19_PENALTY=0.021810
PRIMER_LEFT_19_SEQUENCE=TGTGCTCTTTTCTAATCCAAAGG
PRIMER_RIGHT_19_SEQUENCE=CACACACACACACTTCCAGTTTT
PRIMER_LEFT_19=2264,23
PRIMER_RIGHT_19=2663,23
PRIMER_LEFT_19_TM=59.780
PRIMER_RIGHT_19_TM=60.022
PRIMER_LEFT_19_GC_PERCENT=39.130
PRIMER_RIGHT_19_GC_PERCENT=43.478
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=3.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_19_MISPRIMING_SCORE=12.00, reverse Tigger2 Autonomous DNA transposon
PRIMER_PAIR_19_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_19_END_STABILITY=8.5000
PRIMER_RIGHT_19_END_STABILITY=7.0000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_19=400
=