PRIMER_SEQUENCE_ID=NM_001947
SEQUENCE=ATGCCCTGCAAGAGCGCCGAGTGGCTGCAGGAGGAGCTGGAGGCGCGCGGCGGCGCGTCCTTGCTGCTGCTCGACTGCCGGCCGCACGAGCTCTTCGAGTCGTCGCACATCGAGACGGCCATCAACCTGGCCATCCCGGGCCTCATGTTGCGCCGCCTGCGCAAGGGCAACCTGCCCATCCGCTCCATCATCCCCAACCACGCCGACAAGGAGCGCTTCGCCACGCGCTGCAAGGCGGCCACCGTGCTGCTCTACGACGAGGCCACGGCCGAGTGGCAGCCCGAGCCCGGCGCTCCCGCCTCCGTGCTCGGCCTGCTCCTACAGAAGCTGCGCGACGACGGCTGCCAGGCCTACTACCTCCAAGGTGGTTTCAACAAGTTTCAAACAGAGTACTCTGAGCACTGCGAGACCAACGTGGACAGCTCTTCCTCGCCGAGCAGCTCGCCACCCACCTCAGTGCTGGGCCTGGGGGGCCTGCGCATCAGCTCTGACTGCTCCGACGGCGAGTCGGACCGAGAGCTGCCCAGCAGTGCCACCGAGTCAGACGGCAGCCCTGTGCCATCCAGCCAACCAGCCTTCCCTGTCCAGATCCTGCCCTACCTCTACCTCGGCTGCGCCAAGGACTCCACCAACCTGGACGTGCTCGGCAAGTATGGCATCAAGTATATCCTCAATGTCACACCCAACCTACCCAACGCCTTCGAGCACGGCGGCGAGTTCACCTACAAGCAGATCCCCATCTCTGACCACTGGAGCCAGAACCTCTCCCAGTTCTTCCCTGAGGCCATCAGCTTCATTGACGAAGCCCGCTCCAAGAAGTGTGGTGTCCTGGTGCACTGCCTGGCAGGCATCAGCCGCTCAGTGACGGTCACTGTGGCCTATCTGATGCAGAAGATGAACCTGTCACTCAACGACGCCTACGACTTTGTCAAGAGGAAAAAGTCCAACATCTCGCCCAACTTCAACTTCATGGGGCAGCTGCTGGACTTTGAGCGGACGCTGGGGCTAAGCAGCCCGTGCGACAACCACGCGTCGAGTGAGCAGCTCTACTTTTCCACGCCCACCAACCACAACCTGTTCCCACTCAATACGCTGGAGTCCACGTGA
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 11980, GC content failed 578, low tm 2247, high tm 7148, high any compl 10, high end compl 34, high repeat similarity 19, long poly-x seq 21, ok 1923
PRIMER_RIGHT_EXPLAIN=considered 11983, GC content failed 384, low tm 2438, high tm 7044, high any compl 4, high end compl 10, high repeat similarity 10, long poly-x seq 21, ok 2072
PRIMER_PAIR_EXPLAIN=considered 2741, unacceptable product size 2676, high end compl 5, ok 60
PRIMER_PAIR_PENALTY=0.1967
PRIMER_LEFT_PENALTY=0.002945
PRIMER_RIGHT_PENALTY=0.193718
PRIMER_LEFT_SEQUENCE=ATATCCTCAATGTCACACCCAAC
PRIMER_RIGHT_SEQUENCE=TTTCCTCTTGACAAAGTCGTAGG
PRIMER_LEFT=664,23
PRIMER_RIGHT=938,23
PRIMER_LEFT_TM=59.997
PRIMER_RIGHT_TM=59.806
PRIMER_LEFT_GC_PERCENT=43.478
PRIMER_RIGHT_GC_PERCENT=43.478
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_MISPRIMING_SCORE=11.00, reverse THE1BR THE-1B Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_RIGHT_MISPRIMING_SCORE=11.00, MER4C a consensus
PRIMER_PAIR_MISPRIMING_SCORE=20.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=275
PRIMER_PAIR_PENALTY_1=0.2796
PRIMER_LEFT_1_PENALTY=0.085878
PRIMER_RIGHT_1_PENALTY=0.193718
PRIMER_LEFT_1_SEQUENCE=TACTACCTCCAAGGTGGTTTCAA
PRIMER_RIGHT_1_SEQUENCE=TTTCCTCTTGACAAAGTCGTAGG
PRIMER_LEFT_1=351,23
PRIMER_RIGHT_1=938,23
PRIMER_LEFT_1_TM=59.914
PRIMER_RIGHT_1_TM=59.806
PRIMER_LEFT_1_GC_PERCENT=43.478
PRIMER_RIGHT_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_MISPRIMING_SCORE=11.00, reverse L1MA2 3'-end of L1 repeat (subfamily L1MA2) - a consensus sequence
PRIMER_RIGHT_1_MISPRIMING_SCORE=11.00, MER4C a consensus
PRIMER_PAIR_1_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=588
PRIMER_PAIR_PENALTY_2=0.4664
PRIMER_LEFT_2_PENALTY=0.272721
PRIMER_RIGHT_2_PENALTY=0.193718
PRIMER_LEFT_2_SEQUENCE=CCTACTACCTCCAAGGTGGTTTC
PRIMER_RIGHT_2_SEQUENCE=TTTCCTCTTGACAAAGTCGTAGG
PRIMER_LEFT_2=349,23
PRIMER_RIGHT_2=938,23
PRIMER_LEFT_2_TM=60.273
PRIMER_RIGHT_2_TM=59.806
PRIMER_LEFT_2_GC_PERCENT=52.174
PRIMER_RIGHT_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, reverse LTR5 LTR from human endogenous retrovirus 5' LTR, clone HERV-K18
PRIMER_RIGHT_2_MISPRIMING_SCORE=11.00, MER4C a consensus
PRIMER_PAIR_2_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=590
PRIMER_PAIR_PENALTY_3=0.6031
PRIMER_LEFT_3_PENALTY=0.002945
PRIMER_RIGHT_3_PENALTY=0.600159
PRIMER_LEFT_3_SEQUENCE=ATATCCTCAATGTCACACCCAAC
PRIMER_RIGHT_3_SEQUENCE=GTCGTTGAGTGACAGGTTCATCT
PRIMER_LEFT_3=664,23
PRIMER_RIGHT_3=914,23
PRIMER_LEFT_3_TM=59.997
PRIMER_RIGHT_3_TM=60.600
PRIMER_LEFT_3_GC_PERCENT=43.478
PRIMER_RIGHT_3_GC_PERCENT=47.826
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_MISPRIMING_SCORE=11.00, reverse THE1BR THE-1B Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_3_MISPRIMING_SCORE=21.00, reverse MER4C a consensus
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=251
PRIMER_PAIR_PENALTY_4=0.6165
PRIMER_LEFT_4_PENALTY=0.002945
PRIMER_RIGHT_4_PENALTY=0.613579
PRIMER_LEFT_4_SEQUENCE=ATATCCTCAATGTCACACCCAAC
PRIMER_RIGHT_4_SEQUENCE=TTGAGTGACAGGTTCATCTTCTG
PRIMER_LEFT_4=664,23
PRIMER_RIGHT_4=910,23
PRIMER_LEFT_4_TM=59.997
PRIMER_RIGHT_4_TM=59.386
PRIMER_LEFT_4_GC_PERCENT=43.478
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_MISPRIMING_SCORE=11.00, reverse THE1BR THE-1B Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_4_MISPRIMING_SCORE=20.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=247
PRIMER_PAIR_PENALTY_5=0.6255
PRIMER_LEFT_5_PENALTY=0.431779
PRIMER_RIGHT_5_PENALTY=0.193718
PRIMER_LEFT_5_SEQUENCE=CTACTACCTCCAAGGTGGTTTCA
PRIMER_RIGHT_5_SEQUENCE=TTTCCTCTTGACAAAGTCGTAGG
PRIMER_LEFT_5=350,23
PRIMER_RIGHT_5=938,23
PRIMER_LEFT_5_TM=59.568
PRIMER_RIGHT_5_TM=59.806
PRIMER_LEFT_5_GC_PERCENT=47.826
PRIMER_RIGHT_5_GC_PERCENT=43.478
PRIMER_LEFT_5_SELF_ANY=7.00
PRIMER_RIGHT_5_SELF_ANY=5.00
PRIMER_LEFT_5_SELF_END=2.00
PRIMER_RIGHT_5_SELF_END=2.00
PRIMER_LEFT_5_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_5_MISPRIMING_SCORE=11.00, MER4C a consensus
PRIMER_PAIR_5_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_5_END_STABILITY=7.3000
PRIMER_RIGHT_5_END_STABILITY=6.9000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_5=589
PRIMER_PAIR_PENALTY_6=0.6860
PRIMER_LEFT_6_PENALTY=0.085878
PRIMER_RIGHT_6_PENALTY=0.600159
PRIMER_LEFT_6_SEQUENCE=TACTACCTCCAAGGTGGTTTCAA
PRIMER_RIGHT_6_SEQUENCE=GTCGTTGAGTGACAGGTTCATCT
PRIMER_LEFT_6=351,23
PRIMER_RIGHT_6=914,23
PRIMER_LEFT_6_TM=59.914
PRIMER_RIGHT_6_TM=60.600
PRIMER_LEFT_6_GC_PERCENT=43.478
PRIMER_RIGHT_6_GC_PERCENT=47.826
PRIMER_LEFT_6_SELF_ANY=7.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=3.00
PRIMER_RIGHT_6_SELF_END=2.00
PRIMER_LEFT_6_MISPRIMING_SCORE=11.00, reverse L1MA2 3'-end of L1 repeat (subfamily L1MA2) - a consensus sequence
PRIMER_RIGHT_6_MISPRIMING_SCORE=12.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_6_MISPRIMING_SCORE=21.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_6_END_STABILITY=7.3000
PRIMER_RIGHT_6_END_STABILITY=6.6000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_6=564
PRIMER_PAIR_PENALTY_7=0.6995
PRIMER_LEFT_7_PENALTY=0.085878
PRIMER_RIGHT_7_PENALTY=0.613579
PRIMER_LEFT_7_SEQUENCE=TACTACCTCCAAGGTGGTTTCAA
PRIMER_RIGHT_7_SEQUENCE=TTGAGTGACAGGTTCATCTTCTG
PRIMER_LEFT_7=351,23
PRIMER_RIGHT_7=910,23
PRIMER_LEFT_7_TM=59.914
PRIMER_RIGHT_7_TM=59.386
PRIMER_LEFT_7_GC_PERCENT=43.478
PRIMER_RIGHT_7_GC_PERCENT=43.478
PRIMER_LEFT_7_SELF_ANY=7.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=3.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_MISPRIMING_SCORE=11.00, reverse L1MA2 3'-end of L1 repeat (subfamily L1MA2) - a consensus sequence
PRIMER_RIGHT_7_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_7_MISPRIMING_SCORE=21.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_7_END_STABILITY=7.3000
PRIMER_RIGHT_7_END_STABILITY=7.0000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_7=560
PRIMER_PAIR_PENALTY_8=0.7716
PRIMER_LEFT_8_PENALTY=0.085878
PRIMER_RIGHT_8_PENALTY=0.685716
PRIMER_LEFT_8_SEQUENCE=TACTACCTCCAAGGTGGTTTCAA
PRIMER_RIGHT_8_SEQUENCE=GTATTGAGTGGGAACAGGTTGTG
PRIMER_LEFT_8=351,23
PRIMER_RIGHT_8=1090,23
PRIMER_LEFT_8_TM=59.914
PRIMER_RIGHT_8_TM=60.686
PRIMER_LEFT_8_GC_PERCENT=43.478
PRIMER_RIGHT_8_GC_PERCENT=47.826
PRIMER_LEFT_8_SELF_ANY=7.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=3.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_MISPRIMING_SCORE=11.00, reverse L1MA2 3'-end of L1 repeat (subfamily L1MA2) - a consensus sequence
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence
PRIMER_PAIR_8_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_8_END_STABILITY=7.3000
PRIMER_RIGHT_8_END_STABILITY=7.0000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_8=740
PRIMER_PAIR_PENALTY_9=0.8146
PRIMER_LEFT_9_PENALTY=0.128862
PRIMER_RIGHT_9_PENALTY=0.685716
PRIMER_LEFT_9_SEQUENCE=TTTGTCAAGAGGAAAAAGTCCAA
PRIMER_RIGHT_9_SEQUENCE=GTATTGAGTGGGAACAGGTTGTG
PRIMER_LEFT_9=924,23
PRIMER_RIGHT_9=1090,23
PRIMER_LEFT_9_TM=60.129
PRIMER_RIGHT_9_TM=60.686
PRIMER_LEFT_9_GC_PERCENT=34.783
PRIMER_RIGHT_9_GC_PERCENT=47.826
PRIMER_LEFT_9_SELF_ANY=5.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_MISPRIMING_SCORE=12.00, L1MA2 3'-end of L1 repeat (subfamily L1MA2) - a consensus sequence
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence
PRIMER_PAIR_9_MISPRIMING_SCORE=24.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_9_END_STABILITY=8.5000
PRIMER_RIGHT_9_END_STABILITY=7.0000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_9=167
PRIMER_PAIR_PENALTY_10=0.8729
PRIMER_LEFT_10_PENALTY=0.272721
PRIMER_RIGHT_10_PENALTY=0.600159
PRIMER_LEFT_10_SEQUENCE=CCTACTACCTCCAAGGTGGTTTC
PRIMER_RIGHT_10_SEQUENCE=GTCGTTGAGTGACAGGTTCATCT
PRIMER_LEFT_10=349,23
PRIMER_RIGHT_10=914,23
PRIMER_LEFT_10_TM=60.273
PRIMER_RIGHT_10_TM=60.600
PRIMER_LEFT_10_GC_PERCENT=52.174
PRIMER_RIGHT_10_GC_PERCENT=47.826
PRIMER_LEFT_10_SELF_ANY=7.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=2.00
PRIMER_LEFT_10_MISPRIMING_SCORE=12.00, reverse LTR5 LTR from human endogenous retrovirus 5' LTR, clone HERV-K18
PRIMER_RIGHT_10_MISPRIMING_SCORE=12.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_10_MISPRIMING_SCORE=21.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_10_END_STABILITY=6.7000
PRIMER_RIGHT_10_END_STABILITY=6.6000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_10=566
PRIMER_PAIR_PENALTY_11=0.8863
PRIMER_LEFT_11_PENALTY=0.272721
PRIMER_RIGHT_11_PENALTY=0.613579
PRIMER_LEFT_11_SEQUENCE=CCTACTACCTCCAAGGTGGTTTC
PRIMER_RIGHT_11_SEQUENCE=TTGAGTGACAGGTTCATCTTCTG
PRIMER_LEFT_11=349,23
PRIMER_RIGHT_11=910,23
PRIMER_LEFT_11_TM=60.273
PRIMER_RIGHT_11_TM=59.386
PRIMER_LEFT_11_GC_PERCENT=52.174
PRIMER_RIGHT_11_GC_PERCENT=43.478
PRIMER_LEFT_11_SELF_ANY=7.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_MISPRIMING_SCORE=12.00, reverse LTR5 LTR from human endogenous retrovirus 5' LTR, clone HERV-K18
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_11_MISPRIMING_SCORE=19.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_11_END_STABILITY=6.7000
PRIMER_RIGHT_11_END_STABILITY=7.0000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_11=562
PRIMER_PAIR_PENALTY_12=0.9584
PRIMER_LEFT_12_PENALTY=0.272721
PRIMER_RIGHT_12_PENALTY=0.685716
PRIMER_LEFT_12_SEQUENCE=CCTACTACCTCCAAGGTGGTTTC
PRIMER_RIGHT_12_SEQUENCE=GTATTGAGTGGGAACAGGTTGTG
PRIMER_LEFT_12=349,23
PRIMER_RIGHT_12=1090,23
PRIMER_LEFT_12_TM=60.273
PRIMER_RIGHT_12_TM=60.686
PRIMER_LEFT_12_GC_PERCENT=52.174
PRIMER_RIGHT_12_GC_PERCENT=47.826
PRIMER_LEFT_12_SELF_ANY=7.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_MISPRIMING_SCORE=12.00, reverse LTR5 LTR from human endogenous retrovirus 5' LTR, clone HERV-K18
PRIMER_RIGHT_12_MISPRIMING_SCORE=12.00, L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence
PRIMER_PAIR_12_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_12_END_STABILITY=6.7000
PRIMER_RIGHT_12_END_STABILITY=7.0000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_12=742
PRIMER_PAIR_PENALTY_13=0.9786
PRIMER_LEFT_13_PENALTY=0.784899
PRIMER_RIGHT_13_PENALTY=0.193718
PRIMER_LEFT_13_SEQUENCE=TATATCCTCAATGTCACACCCAA
PRIMER_RIGHT_13_SEQUENCE=TTTCCTCTTGACAAAGTCGTAGG
PRIMER_LEFT_13=663,23
PRIMER_RIGHT_13=938,23
PRIMER_LEFT_13_TM=59.215
PRIMER_RIGHT_13_TM=59.806
PRIMER_LEFT_13_GC_PERCENT=39.130
PRIMER_RIGHT_13_GC_PERCENT=43.478
PRIMER_LEFT_13_SELF_ANY=5.00
PRIMER_RIGHT_13_SELF_ANY=5.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=2.00
PRIMER_LEFT_13_MISPRIMING_SCORE=12.00, reverse THE1BR THE-1B Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_RIGHT_13_MISPRIMING_SCORE=11.00, MER4C a consensus
PRIMER_PAIR_13_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_13_END_STABILITY=10.0000
PRIMER_RIGHT_13_END_STABILITY=6.9000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_13=276
PRIMER_PAIR_PENALTY_14=0.9800
PRIMER_LEFT_14_PENALTY=0.002945
PRIMER_RIGHT_14_PENALTY=0.977043
PRIMER_LEFT_14_SEQUENCE=ATATCCTCAATGTCACACCCAAC
PRIMER_RIGHT_14_SEQUENCE=CGAGATGTTGGACTTTTTCCTCT
PRIMER_LEFT_14=664,23
PRIMER_RIGHT_14=953,23
PRIMER_LEFT_14_TM=59.997
PRIMER_RIGHT_14_TM=60.977
PRIMER_LEFT_14_GC_PERCENT=43.478
PRIMER_RIGHT_14_GC_PERCENT=43.478
PRIMER_LEFT_14_SELF_ANY=5.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_MISPRIMING_SCORE=11.00, reverse THE1BR THE-1B Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_RIGHT_14_MISPRIMING_SCORE=12.00, MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_PAIR_14_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_14_END_STABILITY=8.2000
PRIMER_RIGHT_14_END_STABILITY=7.9000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_14=290
PRIMER_PAIR_PENALTY_15=1.0319
PRIMER_LEFT_15_PENALTY=0.431779
PRIMER_RIGHT_15_PENALTY=0.600159
PRIMER_LEFT_15_SEQUENCE=CTACTACCTCCAAGGTGGTTTCA
PRIMER_RIGHT_15_SEQUENCE=GTCGTTGAGTGACAGGTTCATCT
PRIMER_LEFT_15=350,23
PRIMER_RIGHT_15=914,23
PRIMER_LEFT_15_TM=59.568
PRIMER_RIGHT_15_TM=60.600
PRIMER_LEFT_15_GC_PERCENT=47.826
PRIMER_RIGHT_15_GC_PERCENT=47.826
PRIMER_LEFT_15_SELF_ANY=7.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=2.00
PRIMER_RIGHT_15_SELF_END=2.00
PRIMER_LEFT_15_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_15_MISPRIMING_SCORE=12.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_15_MISPRIMING_SCORE=21.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_15_END_STABILITY=7.3000
PRIMER_RIGHT_15_END_STABILITY=6.6000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_15=565
PRIMER_PAIR_PENALTY_16=1.0454
PRIMER_LEFT_16_PENALTY=0.431779
PRIMER_RIGHT_16_PENALTY=0.613579
PRIMER_LEFT_16_SEQUENCE=CTACTACCTCCAAGGTGGTTTCA
PRIMER_RIGHT_16_SEQUENCE=TTGAGTGACAGGTTCATCTTCTG
PRIMER_LEFT_16=350,23
PRIMER_RIGHT_16=910,23
PRIMER_LEFT_16_TM=59.568
PRIMER_RIGHT_16_TM=59.386
PRIMER_LEFT_16_GC_PERCENT=47.826
PRIMER_RIGHT_16_GC_PERCENT=43.478
PRIMER_LEFT_16_SELF_ANY=7.00
PRIMER_RIGHT_16_SELF_ANY=3.00
PRIMER_LEFT_16_SELF_END=2.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_16_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_16_MISPRIMING_SCORE=20.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_16_END_STABILITY=7.3000
PRIMER_RIGHT_16_END_STABILITY=7.0000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_16=561
PRIMER_PAIR_PENALTY_17=1.0629
PRIMER_LEFT_17_PENALTY=0.085878
PRIMER_RIGHT_17_PENALTY=0.977043
PRIMER_LEFT_17_SEQUENCE=TACTACCTCCAAGGTGGTTTCAA
PRIMER_RIGHT_17_SEQUENCE=CGAGATGTTGGACTTTTTCCTCT
PRIMER_LEFT_17=351,23
PRIMER_RIGHT_17=953,23
PRIMER_LEFT_17_TM=59.914
PRIMER_RIGHT_17_TM=60.977
PRIMER_LEFT_17_GC_PERCENT=43.478
PRIMER_RIGHT_17_GC_PERCENT=43.478
PRIMER_LEFT_17_SELF_ANY=7.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=3.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_MISPRIMING_SCORE=11.00, reverse L1MA2 3'-end of L1 repeat (subfamily L1MA2) - a consensus sequence
PRIMER_RIGHT_17_MISPRIMING_SCORE=12.00, MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_PAIR_17_MISPRIMING_SCORE=21.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_17_END_STABILITY=7.3000
PRIMER_RIGHT_17_END_STABILITY=7.9000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_17=603
PRIMER_PAIR_PENALTY_18=1.0924
PRIMER_LEFT_18_PENALTY=0.002945
PRIMER_RIGHT_18_PENALTY=1.089431
PRIMER_LEFT_18_SEQUENCE=ATATCCTCAATGTCACACCCAAC
PRIMER_RIGHT_18_SEQUENCE=CGTTGAGTGACAGGTTCATCTTC
PRIMER_LEFT_18=664,23
PRIMER_RIGHT_18=912,23
PRIMER_LEFT_18_TM=59.997
PRIMER_RIGHT_18_TM=61.089
PRIMER_LEFT_18_GC_PERCENT=43.478
PRIMER_RIGHT_18_GC_PERCENT=47.826
PRIMER_LEFT_18_SELF_ANY=5.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=2.00
PRIMER_LEFT_18_MISPRIMING_SCORE=11.00, reverse THE1BR THE-1B Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_RIGHT_18_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_18_MISPRIMING_SCORE=20.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_18_END_STABILITY=8.2000
PRIMER_RIGHT_18_END_STABILITY=6.7000
PRIMER_PAIR_18_COMPL_ANY=6.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_18=249
PRIMER_PAIR_PENALTY_19=1.0924
PRIMER_LEFT_19_PENALTY=0.002945
PRIMER_RIGHT_19_PENALTY=1.089431
PRIMER_LEFT_19_SEQUENCE=ATATCCTCAATGTCACACCCAAC
PRIMER_RIGHT_19_SEQUENCE=TCGTTGAGTGACAGGTTCATCTT
PRIMER_LEFT_19=664,23
PRIMER_RIGHT_19=913,23
PRIMER_LEFT_19_TM=59.997
PRIMER_RIGHT_19_TM=61.089
PRIMER_LEFT_19_GC_PERCENT=43.478
PRIMER_RIGHT_19_GC_PERCENT=43.478
PRIMER_LEFT_19_SELF_ANY=5.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_MISPRIMING_SCORE=11.00, reverse THE1BR THE-1B Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_RIGHT_19_MISPRIMING_SCORE=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_19_MISPRIMING_SCORE=20.00, reverse MER4C a consensus
PRIMER_LEFT_19_END_STABILITY=8.2000
PRIMER_RIGHT_19_END_STABILITY=6.6000
PRIMER_PAIR_19_COMPL_ANY=6.00
PRIMER_PAIR_19_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_19=250
=