PRIMER_SEQUENCE_ID=NM_002829
SEQUENCE=CTGCAGGTTATTCAGCGATAGTTATGACCTCCCGGTTACGTGCGTTGGGTGGAAGAATTAATAATATACGCACCTCGGAGTTACCCAAAGAGAAAACTCGATCAGAAGTCATTTGCAGCATCCACTTTTTAGATGGCGTGGTACAGACCTTTAAAGTTACTAAACAAGACACTGGCCAGGTTCTTCTGGATATGGTGCACAACCACCTGGGTGTGACTGAAAAGGAATATTTTGGTTTACAGCATGATGACGACTCCGTGGACTCTCCTAGATGGCTGGAAGCAAGCAAAGCCATCAGGAAGCAGTTAAAAGGAGGTTTCCCCTGTACCCTGCATTTTCGAGTAAGATTTTTTATACCTGATCCCAACACACTGCAGCAAGAACAAACCAGGCACTTGTATTTCTTACAACTGAAGATGGATATTTGCGAAGGAAGGTTAACCTGCCCTCTTAACTCAGCAGTGGTTCTAGCGTCCTATGCCGTACAATCTCATTTTGGAGACTATAATTCTTCCATACATCATCCAGGCTATCTTTCCGATAGTCACTTTATACCCGATCAAAATGAGGACTTTTTAACAAAAGTCGAATCTCTGCATGAGCAGCACAGTGGGCTAAAACAATCAGAAGCAGAATCCTGCTATATCAACATAGCGCGGACCCTCGACTTCTATGGAGTAGAACTGCACAGTGGTAGGGATCTGCACAATTTAGACCTAATGATTGGAATTGCTTCCGCGGGTGTTGCTGTGTACCGAAAATACATTTGCACAAGTTTCTATCCTTGGGTGAACATTCTCAAAATTTCTTTCAAAAGGAAAAAGTTCTTCATACATCAGCGACAGAAACAGGCTGAATCCAGGGAACATATTGTGGCCTTCAACATGCTGAATTACCGATCTTGCAAAAACTTGTGGAAATCCTGTGTTGAGCACCATACGTTCTTTCAGGCAAAGAAGCTACTACCTCAGGAAAAGAATGTTCTGTCTCAGTACTGGACTATGGGCTCTCGGAACACCAAAAAGTCGGTAAATAACCAATATTGCAAAAAGGTGATTGGCGGGATGGTGTGGAACCCAGCCATGCGGAGATCCTTATCAGTGGAGCACTTAGAAACCAAGAGTCTGCCTTCTCGTTCCCCTCCCATTACTCCCAACTGGCGAAGTCCTCGGCTCCGGCACGAAATCCGAAAGCCACGCCACTCTTCTGCAGATAACCTTGCAAATGAAATGACCTACATCACGGAAACGGAAGATGTATTTTACACGTACAAGGGCTCTCTGGCCCCTCAAGACAGCGATTCTGAAGTTTCTCAGAACCGAAGCCCGCACCAAGAGAGTTTATCCGAGAACAATCCGGCACAAAGCTACCTGACCCAGAAGTCATCCAGTTCTGTGTCTCCATCTTCAAATGCTCCAGGCTCCTGCTCACCTGACGGCGTTGATCAGCAGCTCTTAGATGACTTCCACAGGGTGACCAAAGGGGGCTCCACCGAGGACGCCAGCCAGTACTACTGTGACAAGAATGATAATGGTGACAGCTACTTAGTCTTGATCCGTATCACACCAGATGAAGATGGAAAATTTGGATTTAATCTTAAGGGAGGAGTGGATCAAAAGATGCCTCTTGTGGTATCAAGGATAAACCCAGAGTCACCTGCGGACACCTGCATTCCTAAGCTGAACGAAGGGGATCAAATCGTGTTAATCAATGGCCGGGACATCTCAGAACACACGCATGACCAAGTGGTGATGTTCATCAAAGCCAGCCGGGAGTCCCACTCACGGGAGCTGGCCCTGGTGATCAGGAGGAGAGCTGTCCGCTCATTTGCTGACTTCAAGTCTGAAGATGAACTGAACCAGCTTTTCCCCGAAGCCATTTTCCCCATGTGTCCGGAGGGTGGGGACACTTTGGAGGGATCCATGGCACAGCTAAAGAAGGGCCTCGAAAGCGGGACGGTGCTGATCCAGTTTGAGCAACTCTACAGAAAAAAGCCAGGTTTGGCCATCACGTTTGCAAAGCTGCCTCAAAATTTGGACAAAAACCGATATAAAGATGTGCTGCCTTATGACACCACCCGGGTATTATTGCAGGGAAATGAAGATTATATTAATGCAAGTTACGTGAACATGGAAATTCCTGCTGCTAACCTTGTGAACAAGTACATCGCCACTCAGGGGCCCCTGCCGCATACCTGTGCACAGTTTTGGCAGGTTGTCTGGGATCAGAAGTTGTCACTCATTGTCATGTTGACGACTCTCACAGAACGAGGGCGGACCAAATGTCACCAGTACTGGCCAGATCCCCCCGACGTCATGAACCACGGCGGCTTTCACATCCAGTGTCAGTCAGAGGACTGCACCATCGCCTATGTGTCCCGAGAAATGCTGGTCACAAACACCCAGACCGGGGAAGAACACACAGTGACACATCTCCAGTACGTCGCATGGCCTGACCACGGTGTGCCCGATGACTCCTCCGACTTTCTGGAATTTGTAAACTATGTGAGGTCTCTGAGAGTGGACAGCGAGCCTCTCCTAGTTCACTGCAGTGCTGGAATAGGTCGAACCGGTGTGTTGGTCACTATGGAAACAGCCATGTGCCTAACTGAGAGGAACCTGCCCATTTACCCACTGGATATTGTCCGAAAAATGCGAGACCAGCGCGCCATGATGGTGCAGACATCAAGCCAGTACAAGTTTGTGTGTGAAGCGATTCTTCGTGTGTATGAAGAAGGTTTAGTCCAAATGCTGGATCCTAGTTAAGACAACTGTGAAAAAGTTCATTCCTCTTTCCCAAGGGCATCCTCCTTGAAAGAGGAGGACAGACCTCTCTGGAAGCAGCAAGAGGAACCAGTAGCTGTGGGAAAGGAATGGGCACCTCTGAACCCAGGCACTTTAAACTTCTATAGAAAAGATATCGTGTACATAGGAACTGGTGTAGATAAGCATGCAATTATGGCATCATTTAGGCCTGTATTTCTATGGAAAGATACAAAAAGGATCTCAGTTTGGGGCCTGTCCTAATGCCTTCTTCCCTAACATCACCACACACACCCCTGTCGGCATCCTGGAGCAATTGAGACCGGACACCCACAGAGCTGTTGTCCTCCCAGCAACAAGATGGTGTGGTTATCTTGGGTCATTTGGATGTTTTGTTTGTTTCTGTGTGTCAGACTGTAAGGGCTGAGCTTTCTGTGCTTCTAGGTGGAGCTGGAACAATTCAGATTCACCCGCCCTGATGCTAAGGAAACCCTGACGTATGTACTAGATGGCAGGGCACTGGGGGTCAGGCTGAAGGCTGAGCAACACCTCTCTGCCCTCCCTCCCTTTGTCCCATCTCCCAGCGACTTCCAATATTCATGTTTCTGAGAATTGTGTCCCTCTTCAGGTTCCCTCTTGGTGCCTAACCTGGATTAGTAATGTGCATTCAGGTGAATTTTCAGCTGAGGCTCTGAGAACTGGTACTCTCAGTGTGTTCTGGTCATCTTGTGGCTTAGTTGTAGAAGCAGGTGTGTCTCTTGCCTCTGCTTGCCTCCTACTGCACACTCAGCACCCAGGACTGGAATCACCGACTACTGAATCTCCTACATGTATTGCTGCTACTTCAAGCTCCTCCACTTGAAACCTTATGATTTTCCCAAGGGGAGATGGGACAGTGTCATCTAAATATTCCGAATGTTTGGCCTTCTGAGAAAAGAGCTTCTAGTAATTGAACCATGGGAAACCCAGCTTCTGGAGGGTTGGCCGTGGGGCTGTGTACATGTGTGTGCCCAGGGGTGAGTGTTTCTCAGGATTCCTAACGATTCAAATTACCGTTGAGTATATATAAAGAATGAGTCTCTGTATGGAAGAACAAATGTGTGCATTCACCCCCAGTCACAATGGTCTCCATTGCATTTCAAAGGAGAGGATCAGACTATCTGAATATAAACACAATCTGATGTTAATTTATTCTAAGAACACCATCATTTTGATTGTCCTAAAGAATTCTGCCTTTGTGAATACCGTGTTAAATTTTTTTAAATTTGTGACAGGATTGTAGCAAATTATTATTTAAGGAAAATAAATTGTGTAAACATTTAAAAAAAAAAAAAAAAAAAAA
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 47549, GC content failed 327, low tm 21449, high tm 14583, high any compl 9, high end compl 20, high repeat similarity 26, long poly-x seq 68, ok 11067
PRIMER_RIGHT_EXPLAIN=considered 47484, GC content failed 876, low tm 21244, high tm 14331, high any compl 6, high end compl 10, high repeat similarity 8, long poly-x seq 81, ok 10928
PRIMER_PAIR_EXPLAIN=considered 634, unacceptable product size 582, high end compl 5, ok 47
PRIMER_PAIR_PENALTY=0.0348
PRIMER_LEFT_PENALTY=0.023182
PRIMER_RIGHT_PENALTY=0.011619
PRIMER_LEFT_SEQUENCE=TAATGCCTTCTTCCCTAACATCA
PRIMER_RIGHT_SEQUENCE=AGATTCAGTAGTCGGTGATTCCA
PRIMER_LEFT=3023,23
PRIMER_RIGHT=3584,23
PRIMER_LEFT_TM=59.977
PRIMER_RIGHT_TM=60.012
PRIMER_LEFT_GC_PERCENT=39.130
PRIMER_RIGHT_GC_PERCENT=43.478
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_MISPRIMING_SCORE=12.00, MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_PAIR_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=562
PRIMER_PAIR_PENALTY_1=0.0348
PRIMER_LEFT_1_PENALTY=0.023182
PRIMER_RIGHT_1_PENALTY=0.011619
PRIMER_LEFT_1_SEQUENCE=TAATGCCTTCTTCCCTAACATCA
PRIMER_RIGHT_1_SEQUENCE=GATTCAGTAGTCGGTGATTCCAG
PRIMER_LEFT_1=3023,23
PRIMER_RIGHT_1=3583,23
PRIMER_LEFT_1_TM=59.977
PRIMER_RIGHT_1_TM=60.012
PRIMER_LEFT_1_GC_PERCENT=39.130
PRIMER_RIGHT_1_GC_PERCENT=47.826
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_1_MISPRIMING_SCORE=11.00, MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_PAIR_1_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=561
PRIMER_PAIR_PENALTY_2=0.0449
PRIMER_LEFT_2_PENALTY=0.014220
PRIMER_RIGHT_2_PENALTY=0.030702
PRIMER_LEFT_2_SEQUENCE=AAAGATGCCTCTTGTGGTATCAA
PRIMER_RIGHT_2_SEQUENCE=GACAACTTCTGATCCCAGACAAC
PRIMER_LEFT_2=1615,23
PRIMER_RIGHT_2=2235,23
PRIMER_LEFT_2_TM=60.014
PRIMER_RIGHT_2_TM=60.031
PRIMER_LEFT_2_GC_PERCENT=39.130
PRIMER_RIGHT_2_GC_PERCENT=47.826
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_MISPRIMING_SCORE=11.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_2_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_2_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=621
PRIMER_PAIR_PENALTY_3=0.0539
PRIMER_LEFT_3_PENALTY=0.030702
PRIMER_RIGHT_3_PENALTY=0.023182
PRIMER_LEFT_3_SEQUENCE=GTTGTCTGGGATCAGAAGTTGTC
PRIMER_RIGHT_3_SEQUENCE=TGATGTTAGGGAAGAAGGCATTA
PRIMER_LEFT_3=2213,23
PRIMER_RIGHT_3=3045,23
PRIMER_LEFT_3_TM=60.031
PRIMER_RIGHT_3_TM=59.977
PRIMER_LEFT_3_GC_PERCENT=47.826
PRIMER_RIGHT_3_GC_PERCENT=39.130
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_MISPRIMING_SCORE=11.00, MER21B a consensus
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, reverse LOR1 Human low copy repetitive sequence - a consensus
PRIMER_PAIR_3_MISPRIMING_SCORE=20.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=833
PRIMER_PAIR_PENALTY_4=0.0719
PRIMER_LEFT_4_PENALTY=0.030702
PRIMER_RIGHT_4_PENALTY=0.041227
PRIMER_LEFT_4_SEQUENCE=GTTGTCTGGGATCAGAAGTTGTC
PRIMER_RIGHT_4_SEQUENCE=GCATGCTTATCTACACCAGTTCC
PRIMER_LEFT_4=2213,23
PRIMER_RIGHT_4=2952,23
PRIMER_LEFT_4_TM=60.031
PRIMER_RIGHT_4_TM=60.041
PRIMER_LEFT_4_GC_PERCENT=47.826
PRIMER_RIGHT_4_GC_PERCENT=47.826
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_MISPRIMING_SCORE=11.00, MER21B a consensus
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_PAIR_4_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=740
PRIMER_PAIR_PENALTY_5=0.0785
PRIMER_LEFT_5_PENALTY=0.014220
PRIMER_RIGHT_5_PENALTY=0.064245
PRIMER_LEFT_5_SEQUENCE=AAAGATGCCTCTTGTGGTATCAA
PRIMER_RIGHT_5_SEQUENCE=AAGCTGGTTCAGTTCATCTTCAG
PRIMER_LEFT_5=1615,23
PRIMER_RIGHT_5=1864,23
PRIMER_LEFT_5_TM=60.014
PRIMER_RIGHT_5_TM=59.936
PRIMER_LEFT_5_GC_PERCENT=39.130
PRIMER_RIGHT_5_GC_PERCENT=43.478
PRIMER_LEFT_5_SELF_ANY=8.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=2.00
PRIMER_RIGHT_5_SELF_END=2.00
PRIMER_LEFT_5_MISPRIMING_SCORE=11.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_5_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_5_MISPRIMING_SCORE=21.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_5_END_STABILITY=6.9000
PRIMER_RIGHT_5_END_STABILITY=7.0000
PRIMER_PAIR_5_COMPL_ANY=6.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_5=250
PRIMER_PAIR_PENALTY_6=0.0790
PRIMER_LEFT_6_PENALTY=0.067424
PRIMER_RIGHT_6_PENALTY=0.011619
PRIMER_LEFT_6_SEQUENCE=GTATGTACTAGATGGCAGGGCAC
PRIMER_RIGHT_6_SEQUENCE=GATTCAGTAGTCGGTGATTCCAG
PRIMER_LEFT_6=3259,23
PRIMER_RIGHT_6=3583,23
PRIMER_LEFT_6_TM=59.933
PRIMER_RIGHT_6_TM=60.012
PRIMER_LEFT_6_GC_PERCENT=52.174
PRIMER_RIGHT_6_GC_PERCENT=47.826
PRIMER_LEFT_6_SELF_ANY=5.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=2.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_6_MISPRIMING_SCORE=11.00, MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_PAIR_6_MISPRIMING_SCORE=22.00, MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_LEFT_6_END_STABILITY=9.4000
PRIMER_RIGHT_6_END_STABILITY=8.2000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_6=325
PRIMER_PAIR_PENALTY_7=0.0790
PRIMER_LEFT_7_PENALTY=0.067424
PRIMER_RIGHT_7_PENALTY=0.011619
PRIMER_LEFT_7_SEQUENCE=GTATGTACTAGATGGCAGGGCAC
PRIMER_RIGHT_7_SEQUENCE=AGATTCAGTAGTCGGTGATTCCA
PRIMER_LEFT_7=3259,23
PRIMER_RIGHT_7=3584,23
PRIMER_LEFT_7_TM=59.933
PRIMER_RIGHT_7_TM=60.012
PRIMER_LEFT_7_GC_PERCENT=52.174
PRIMER_RIGHT_7_GC_PERCENT=43.478
PRIMER_LEFT_7_SELF_ANY=5.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=2.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_7_MISPRIMING_SCORE=12.00, MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_PAIR_7_MISPRIMING_SCORE=23.00, MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_LEFT_7_END_STABILITY=9.4000
PRIMER_RIGHT_7_END_STABILITY=8.5000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_7=326
PRIMER_PAIR_PENALTY_8=0.0810
PRIMER_LEFT_8_PENALTY=0.030702
PRIMER_RIGHT_8_PENALTY=0.050278
PRIMER_LEFT_8_SEQUENCE=GTTGTCTGGGATCAGAAGTTGTC
PRIMER_RIGHT_8_SEQUENCE=AGAAGTTTAAAGTGCCTGGGTTC
PRIMER_LEFT_8=2213,23
PRIMER_RIGHT_8=2906,23
PRIMER_LEFT_8_TM=60.031
PRIMER_RIGHT_8_TM=60.050
PRIMER_LEFT_8_GC_PERCENT=47.826
PRIMER_RIGHT_8_GC_PERCENT=43.478
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_MISPRIMING_SCORE=11.00, MER21B a consensus
PRIMER_RIGHT_8_MISPRIMING_SCORE=11.00, MER4C a consensus
PRIMER_PAIR_8_MISPRIMING_SCORE=20.00, MER34 a consensus
PRIMER_LEFT_8_END_STABILITY=6.7000
PRIMER_RIGHT_8_END_STABILITY=7.9000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_8=694
PRIMER_PAIR_PENALTY_9=0.0852
PRIMER_LEFT_9_PENALTY=0.073619
PRIMER_RIGHT_9_PENALTY=0.011619
PRIMER_LEFT_9_SEQUENCE=GACGTATGTACTAGATGGCAGGG
PRIMER_RIGHT_9_SEQUENCE=GATTCAGTAGTCGGTGATTCCAG
PRIMER_LEFT_9=3256,23
PRIMER_RIGHT_9=3583,23
PRIMER_LEFT_9_TM=59.926
PRIMER_RIGHT_9_TM=60.012
PRIMER_LEFT_9_GC_PERCENT=52.174
PRIMER_RIGHT_9_GC_PERCENT=47.826
PRIMER_LEFT_9_SELF_ANY=5.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_MISPRIMING_SCORE=11.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_9_MISPRIMING_SCORE=11.00, MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_PAIR_9_MISPRIMING_SCORE=21.00, MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_LEFT_9_END_STABILITY=9.7000
PRIMER_RIGHT_9_END_STABILITY=8.2000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_9=328
PRIMER_PAIR_PENALTY_10=0.0852
PRIMER_LEFT_10_PENALTY=0.073619
PRIMER_RIGHT_10_PENALTY=0.011619
PRIMER_LEFT_10_SEQUENCE=GACGTATGTACTAGATGGCAGGG
PRIMER_RIGHT_10_SEQUENCE=AGATTCAGTAGTCGGTGATTCCA
PRIMER_LEFT_10=3256,23
PRIMER_RIGHT_10=3584,23
PRIMER_LEFT_10_TM=59.926
PRIMER_RIGHT_10_TM=60.012
PRIMER_LEFT_10_GC_PERCENT=52.174
PRIMER_RIGHT_10_GC_PERCENT=43.478
PRIMER_LEFT_10_SELF_ANY=5.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_MISPRIMING_SCORE=11.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_10_MISPRIMING_SCORE=12.00, MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_PAIR_10_MISPRIMING_SCORE=22.00, MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_LEFT_10_END_STABILITY=9.7000
PRIMER_RIGHT_10_END_STABILITY=8.5000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_10=329
PRIMER_PAIR_PENALTY_11=0.0852
PRIMER_LEFT_11_PENALTY=0.073619
PRIMER_RIGHT_11_PENALTY=0.011619
PRIMER_LEFT_11_SEQUENCE=CCCTGACGTATGTACTAGATGGC
PRIMER_RIGHT_11_SEQUENCE=GATTCAGTAGTCGGTGATTCCAG
PRIMER_LEFT_11=3252,23
PRIMER_RIGHT_11=3583,23
PRIMER_LEFT_11_TM=59.926
PRIMER_RIGHT_11_TM=60.012
PRIMER_LEFT_11_GC_PERCENT=52.174
PRIMER_RIGHT_11_GC_PERCENT=47.826
PRIMER_LEFT_11_SELF_ANY=5.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=2.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_MISPRIMING_SCORE=12.00, L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_RIGHT_11_MISPRIMING_SCORE=11.00, MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_PAIR_11_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_11_END_STABILITY=9.6000
PRIMER_RIGHT_11_END_STABILITY=8.2000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_11=332
PRIMER_PAIR_PENALTY_12=0.0852
PRIMER_LEFT_12_PENALTY=0.073619
PRIMER_RIGHT_12_PENALTY=0.011619
PRIMER_LEFT_12_SEQUENCE=CCCTGACGTATGTACTAGATGGC
PRIMER_RIGHT_12_SEQUENCE=AGATTCAGTAGTCGGTGATTCCA
PRIMER_LEFT_12=3252,23
PRIMER_RIGHT_12=3584,23
PRIMER_LEFT_12_TM=59.926
PRIMER_RIGHT_12_TM=60.012
PRIMER_LEFT_12_GC_PERCENT=52.174
PRIMER_RIGHT_12_GC_PERCENT=43.478
PRIMER_LEFT_12_SELF_ANY=5.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=2.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_MISPRIMING_SCORE=12.00, L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_RIGHT_12_MISPRIMING_SCORE=12.00, MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_PAIR_12_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_12_END_STABILITY=9.6000
PRIMER_RIGHT_12_END_STABILITY=8.5000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_12=333
PRIMER_PAIR_PENALTY_13=0.0931
PRIMER_LEFT_13_PENALTY=0.011619
PRIMER_RIGHT_13_PENALTY=0.081493
PRIMER_LEFT_13_SEQUENCE=TGGAATCACCGACTACTGAATCT
PRIMER_RIGHT_13_SEQUENCE=TGCACACATTTGTTCTTCCATAC
PRIMER_LEFT_13=3562,23
PRIMER_RIGHT_13=3866,23
PRIMER_LEFT_13_TM=60.012
PRIMER_RIGHT_13_TM=59.919
PRIMER_LEFT_13_GC_PERCENT=43.478
PRIMER_RIGHT_13_GC_PERCENT=39.130
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=6.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_MISPRIMING_SCORE=12.00, MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_RIGHT_13_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_13_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_13_END_STABILITY=6.6000
PRIMER_RIGHT_13_END_STABILITY=5.6000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_13=305
PRIMER_PAIR_PENALTY_14=0.0931
PRIMER_LEFT_14_PENALTY=0.011619
PRIMER_RIGHT_14_PENALTY=0.081493
PRIMER_LEFT_14_SEQUENCE=TGGAATCACCGACTACTGAATCT
PRIMER_RIGHT_14_SEQUENCE=GCACACATTTGTTCTTCCATACA
PRIMER_LEFT_14=3562,23
PRIMER_RIGHT_14=3865,23
PRIMER_LEFT_14_TM=60.012
PRIMER_RIGHT_14_TM=59.919
PRIMER_LEFT_14_GC_PERCENT=43.478
PRIMER_RIGHT_14_GC_PERCENT=39.130
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_MISPRIMING_SCORE=12.00, MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_RIGHT_14_MISPRIMING_SCORE=12.00, L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_PAIR_14_MISPRIMING_SCORE=21.00, MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_LEFT_14_END_STABILITY=6.6000
PRIMER_RIGHT_14_END_STABILITY=5.6000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_14=304
PRIMER_PAIR_PENALTY_15=0.0931
PRIMER_LEFT_15_PENALTY=0.011619
PRIMER_RIGHT_15_PENALTY=0.081493
PRIMER_LEFT_15_SEQUENCE=CTGGAATCACCGACTACTGAATC
PRIMER_RIGHT_15_SEQUENCE=TGCACACATTTGTTCTTCCATAC
PRIMER_LEFT_15=3561,23
PRIMER_RIGHT_15=3866,23
PRIMER_LEFT_15_TM=60.012
PRIMER_RIGHT_15_TM=59.919
PRIMER_LEFT_15_GC_PERCENT=47.826
PRIMER_RIGHT_15_GC_PERCENT=39.130
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=3.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_MISPRIMING_SCORE=11.00, MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_RIGHT_15_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_15_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_15_END_STABILITY=6.6000
PRIMER_RIGHT_15_END_STABILITY=5.6000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_15=306
PRIMER_PAIR_PENALTY_16=0.0931
PRIMER_LEFT_16_PENALTY=0.011619
PRIMER_RIGHT_16_PENALTY=0.081493
PRIMER_LEFT_16_SEQUENCE=CTGGAATCACCGACTACTGAATC
PRIMER_RIGHT_16_SEQUENCE=GCACACATTTGTTCTTCCATACA
PRIMER_LEFT_16=3561,23
PRIMER_RIGHT_16=3865,23
PRIMER_LEFT_16_TM=60.012
PRIMER_RIGHT_16_TM=59.919
PRIMER_LEFT_16_GC_PERCENT=47.826
PRIMER_RIGHT_16_GC_PERCENT=39.130
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=6.00
PRIMER_LEFT_16_SELF_END=3.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_MISPRIMING_SCORE=11.00, MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_RIGHT_16_MISPRIMING_SCORE=12.00, L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_PAIR_16_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_16_END_STABILITY=6.6000
PRIMER_RIGHT_16_END_STABILITY=5.6000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_16=305
PRIMER_PAIR_PENALTY_17=0.0949
PRIMER_LEFT_17_PENALTY=0.064245
PRIMER_RIGHT_17_PENALTY=0.030702
PRIMER_LEFT_17_SEQUENCE=CTGAAGATGAACTGAACCAGCTT
PRIMER_RIGHT_17_SEQUENCE=GACAACTTCTGATCCCAGACAAC
PRIMER_LEFT_17=1842,23
PRIMER_RIGHT_17=2235,23
PRIMER_LEFT_17_TM=59.936
PRIMER_RIGHT_17_TM=60.031
PRIMER_LEFT_17_GC_PERCENT=43.478
PRIMER_RIGHT_17_GC_PERCENT=47.826
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=3.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_MISPRIMING_SCORE=11.00, reverse LTR9 LTR from human endogenous retrovirus-like sequence (HUERS-P3)
PRIMER_RIGHT_17_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_17_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_17_END_STABILITY=8.2000
PRIMER_RIGHT_17_END_STABILITY=6.4000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_17=394
PRIMER_PAIR_PENALTY_18=0.0968
PRIMER_LEFT_18_PENALTY=0.023182
PRIMER_RIGHT_18_PENALTY=0.073619
PRIMER_LEFT_18_SEQUENCE=TAATGCCTTCTTCCCTAACATCA
PRIMER_RIGHT_18_SEQUENCE=CCCTGCCATCTAGTACATACGTC
PRIMER_LEFT_18=3023,23
PRIMER_RIGHT_18=3278,23
PRIMER_LEFT_18_TM=59.977
PRIMER_RIGHT_18_TM=59.926
PRIMER_LEFT_18_GC_PERCENT=39.130
PRIMER_RIGHT_18_GC_PERCENT=52.174
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=5.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=2.00
PRIMER_LEFT_18_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_18_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_18_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_18_END_STABILITY=6.9000
PRIMER_RIGHT_18_END_STABILITY=7.8000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_18=256
PRIMER_PAIR_PENALTY_19=0.0985
PRIMER_LEFT_19_PENALTY=0.067837
PRIMER_RIGHT_19_PENALTY=0.030702
PRIMER_LEFT_19_SEQUENCE=ACAGCGATTCTGAAGTTTCTCAG
PRIMER_RIGHT_19_SEQUENCE=GACAACTTCTGATCCCAGACAAC
PRIMER_LEFT_19=1293,23
PRIMER_RIGHT_19=2235,23
PRIMER_LEFT_19_TM=60.068
PRIMER_RIGHT_19_TM=60.031
PRIMER_LEFT_19_GC_PERCENT=43.478
PRIMER_RIGHT_19_GC_PERCENT=47.826
PRIMER_LEFT_19_SELF_ANY=5.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=3.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_19_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_19_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_19_END_STABILITY=6.7000
PRIMER_RIGHT_19_END_STABILITY=6.4000
PRIMER_PAIR_19_COMPL_ANY=7.00
PRIMER_PAIR_19_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_19=943
=