PRIMER_SEQUENCE_ID=NM_002830
SEQUENCE=CCTGCGTGTCCCTCTGCGCTCCGACTGGTGCGACTTCTCCCTGCGCTAGCGAGGCAGGGTTTTGGCCTCGCCTCTCGCGAGATCGCCTCCTGTTGCTGCCGCCGCCGCTCCTGGCCACTGACTGGCGGCGCCTGCGCAGCCGCCATGTTCGGTTGCTATGCTGCGGCCTAGGAGAGGGGGTGTGCTTGAGGGAGGAGGAAGAGATAGAGGAGGAGGAGGGGGAGGAAGAGGAGGTGGAGAAGGAGGGGGGTGACTGAGCTCCTCTTGCACTCTCACACACAAACGCTGCCCAGGATTACCCGCCAGCTCACGCCGCGCAGTGCGCTTTTCCGCTCCTCGCGCCCCACCACCAACATTGTTCTCTCAGGACTCCTGGGTCCCAGGGGTCGGAATTGGGCCTGAGCGGGAGAGGAAAGAGACTTGGCTTTGGCCGCGGGGTCGGAGGATTGGGGCCAGGCCCCCTCCCCCACGCACTTTTGGGGGTGTGGATTATCTCATCCCTGCAGGGAGGTAGGAGAGGTCGCCGGCTGCCCGCCTCCCTGCCACCTCCCCAGCGGCGCCGGCCCGCGGCTGCCCAGCAGCATGAGGTGGTGCTGGCGGCTCCGGGTCGTGGCGCGACCGCTGCGGCGGCGGCTGCTCGGGGGGCGCTGAGGTAGCCCCCCGGAGCGGCACGGAGGACGCGCTTCTCCTCTGCGCGCCGGGGCCTCGAGGCTTTTTTTCTCCAGCCGAGAGGACGCGGCTGTGATATACGAAGACTTTGTGTGGACAGTAATGACCTCACGTTTCCGATTGCCTGCTGGCAGAACCTACAATGTACGAGCATCAGAGTTGGCCCGAGACAGACAGCATACTGAAGTGGTTTGCAACATCCTTCTTCTGGATAACACTGTACAAGCTTTCAAAGTCAATAAACATGATCAGGGGCAAGTCTTGTTGGATGTCGTCTTCAAGCATCTAGATTTGACTGAGCAGGACTATTTTGGTTTACAGTTGGCTGATGATTCCACAGATAACCCAAGGTGGCTGGATCCAAACAAACCAATAAGGAAGCAGCTAAAGAGAGGATCTCCTTACAGTTTGAACTTTAGAGTCAAATTTTTTGTAAGTGACCCCAACAAGTTACAAGAAGAATATACAAGGTACCAGTATTTTTTGCAAATTAAACAAGACATTCTTACTGGAAGATTACCCTGTCCTTCTAATACTGCTGCCCTTTTAGCTTCATTTGCTGTTCAGTCTGAACTTGGAGACTACGATCAGTCAGAGAACTTGTCAGGCTACCTCTCAGATTATTCTTTCATTCCTAATCAACCTCAAGATTTTGAAAAAGAAATTGCAAAATTACATCAGCAACACATAGGCTTATCTCCTGCAGAAGCAGAATTTAATTACCTAAACACAGCACGTACCTTAGAACTCTATGGAGTTGAATTCCACTATGCAAGGGATCAGAGTAACAATGAAATTATGATTGGAGTGATGTCAGGAGGAATTCTGATTTATAAGAACAGGGTACGAATGAATACCTTTCCATGGTTGAAGATTGTAAAAATTTCTTTTAAGTGCAAACAGTTTTTTATTCAACTTAGAAAAGAATTGCATGAATCTAGAGAAACATTATTGGGATTTAATATGGTGAATTACAGAGCATGTAAAAATTTGTGGAAAGCATGTGTAGAACATCACACATTCTTCCGTTTGGACAGACCACTTCCACCTCAAAAGAATTTTTTTGCACATTATTTTACATTAGGTTCAAAATTCCGGTACTGTGGGAGAACTGAAGTCCAATCAGTTCAGTATGGCAAAGAAAAGGCAAATAAAGACAGGGTATTTGCAAGATCCCCAAGTAAGCCCTTGGCACGGAAATTAATGGATTGGGAAGTAGTAAGCAGAAATTCAATATCTGATGACAGGTTAGAAACACAAAGTCTTCCATCACGATCTCCACCGGGAACTCCTAATCATCGAAATTCTACATTCACGCAGGAAGGAACCCGGTTACGACCATCTTCAGTTGGTCATTTGGTAGACCATATGGTTCATACTTCCCCAAGCGAAGTGTTTGTAAATCAGAGATCTCCGTCATCAACACAAGCTAATAGCATTGTTCTGGAATCATCACCATCACAAGAGACCCCTGGAGATGGGAAGCCTCCAGCTTTACCACCCAAACAGTCAAAGAAAAACAGTTGGAACCAAATTCATTATTCACATTCGCAACAAGATCTAGAAAGTCATATTAATGAAACATTTGATATTCCATCTTCTCCTGAAAAACCCACTCCTAATGGTGGTATTCCACATGATAATCTTGTCCTAATCAGAATGAAACCTGATGAAAATGGGAGGTTTGGATTCAATGTAAAGGGAGGATATGATCAGAAGATGCCTGTGATTGTGTCTCGAGTAGCACCAGGAACACCTGCTGACCTCTGTGTCCCTAGACTGAATGAAGGGGACCAAGTTGTACTGATCAATGGTCGGGACATTGCAGAACACACTCATGATCAGGTTGTGCTGTTTATTAAAGCTAGTTGTGAGAGACATTCTGGGGAACTCATGCTTCTAGTTCGACCTAATGCTGTATATGATGTAGTGGAAGAAAAGCTAGAAAATGAGCCAGATTTCCAGTATATTCCTGAGAAAGCCCCACTAGATAGTGTGCATCAGGATGACCATTCCCTGCGGGAGTCAATGATCCAGCTAGCTGAGGGGCTTATCACTGGAACAGTCCTGACACAGTTTGATCAACTGTATCGGAAAAAACCTGGAATGACAATGTCCTGTGCCAAATTACCTCAGAATATTTCCAAAAATAGATACAGAGATATTTCGCCTTATGATGCCACACGGGTCATTTTAAAAGGTAATGAAGACTACATCAATGCGAACTATATAAATATGGAAATTCCTTCTTCCAGCATTATAAATCAGTACATTGCTTGTCAAGGGCCATTACCACACACTTGTACAGATTTTTGGCAGATGACTTGGGAACAAGGCTCCTCTATGGTTGTAATGTTGACCACACAAGTTGAACGTGGCAGAGTTAAATGTCACCAATATTGGCCAGAACCCACAGGCAGTTCATCTTATGGATGCTACCAAGTTACCTGCCACTCTGAAGAAGGAAACACTGCCTATATCTTCAGGAAGATGACCCTATTTAACCAAGAGAAAAATGAAAGTCGTCCACTCACTCAGATCCAGTACATAGCCTGGCCTGACCATGGAGTCCCTGATGATTCGAGTGACTTTCTAGATTTTGTTTGTCATGTACGAAACAAGAGGGCTGGCAAGGAAGAACCCGTTGTTGTCCATTGCAGTGCTGGAATCGGAAGAACTGGGGTTCTTATTACTATGGAAACAGCCATGTGTCTCATTGAATGCAATCAGCCAGTTTATCCACTAGATATTGTAAGAACAATGAGAGATCAGCGAGCCATGATGATCCAAACACCTAGTCAATACAGATTTGTATGTGAAGCTATTTTGAAAGTTTATGAAGAAGGCTTTGTTAAACCCTTAACAACATCAACAAATAAATAAGAAAGCAAAAAGATCTGGGATATGTGTTGGAAAACTGCTTTCCCTTATGTTCACTGTGCCATAATGCTGCTCGCAGGAAATGGCATTTTACAAAAAAAAAATGAAGAACTCAAAAAAACTTTGAAAACTTCAGCACTGTTGCACTTTATGTTTTAAAAAATGTCACTCTTTCAAAATCTATAACTCATGTATTTGAAGACTGTTTCATGCTTTGCTCCGAACAAATAGTAAATAACTGAGTATGTTCAGGGTAATTTATGAAATTTTGTGGTGGTGCCATGCAATCCCCTTTTGGTAGAATTGCCACAAACAAGGCTCAAAATTCTCATCATCTCTGTTATACACCTGTATCATGAAAGCAAAAAGAAGTAAACATCAGGAGTCAGCTCTGAAAAAAAAAAAAAAAAAAAA
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 44675, GC content failed 2268, low tm 22213, high tm 10415, high any compl 6, high end compl 28, high repeat similarity 34, long poly-x seq 231, ok 9480
PRIMER_RIGHT_EXPLAIN=considered 44536, GC content failed 2290, low tm 22761, high tm 9729, high any compl 4, high end compl 8, high repeat similarity 13, long poly-x seq 250, ok 9481
PRIMER_PAIR_EXPLAIN=considered 828, unacceptable product size 764, high end compl 8, ok 56
PRIMER_PAIR_PENALTY=0.0252
PRIMER_LEFT_PENALTY=0.008825
PRIMER_RIGHT_PENALTY=0.016351
PRIMER_LEFT_SEQUENCE=CTGGAAGATTACCCTGTCCTTCT
PRIMER_RIGHT_SEQUENCE=TTATTTGCCTTTTCTTTGCCATA
PRIMER_LEFT=1177,23
PRIMER_RIGHT=1822,23
PRIMER_LEFT_TM=60.009
PRIMER_RIGHT_TM=59.984
PRIMER_LEFT_GC_PERCENT=47.826
PRIMER_RIGHT_GC_PERCENT=30.435
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_MISPRIMING_SCORE=12.00, Tigger2 Autonomous DNA transposon
PRIMER_PAIR_MISPRIMING_SCORE=23.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=7.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=646
PRIMER_PAIR_PENALTY_1=0.0562
PRIMER_LEFT_1_PENALTY=0.051149
PRIMER_RIGHT_1_PENALTY=0.005048
PRIMER_LEFT_1_SEQUENCE=CTTTACCACCCAAACAGTCAAAG
PRIMER_RIGHT_1_SEQUENCE=GAGTGGGTTTTTCAGGAGAAGAT
PRIMER_LEFT_1=2161,23
PRIMER_RIGHT_1=2286,23
PRIMER_LEFT_1_TM=59.949
PRIMER_RIGHT_1_TM=59.995
PRIMER_LEFT_1_GC_PERCENT=43.478
PRIMER_RIGHT_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_MISPRIMING_SCORE=11.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_1_MISPRIMING_SCORE=23.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=126
PRIMER_PAIR_PENALTY_2=0.0659
PRIMER_LEFT_2_PENALTY=0.049500
PRIMER_RIGHT_2_PENALTY=0.016351
PRIMER_LEFT_2_SEQUENCE=AGTGACCCCAACAAGTTACAAGA
PRIMER_RIGHT_2_SEQUENCE=TTATTTGCCTTTTCTTTGCCATA
PRIMER_LEFT_2=1104,23
PRIMER_RIGHT_2=1822,23
PRIMER_LEFT_2_TM=59.950
PRIMER_RIGHT_2_TM=59.984
PRIMER_LEFT_2_GC_PERCENT=43.478
PRIMER_RIGHT_2_GC_PERCENT=30.435
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, reverse HY3 Ro(hY3) pseudogene - a consensus
PRIMER_RIGHT_2_MISPRIMING_SCORE=12.00, Tigger2 Autonomous DNA transposon
PRIMER_PAIR_2_MISPRIMING_SCORE=23.00, Tigger2 Autonomous DNA transposon
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=7.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=719
PRIMER_PAIR_PENALTY_3=0.0719
PRIMER_LEFT_3_PENALTY=0.008825
PRIMER_RIGHT_3_PENALTY=0.063026
PRIMER_LEFT_3_SEQUENCE=CTGGAAGATTACCCTGTCCTTCT
PRIMER_RIGHT_3_SEQUENCE=GCCTGACAAGTTCTCTGACTGAT
PRIMER_LEFT_3=1177,23
PRIMER_RIGHT_3=1277,23
PRIMER_LEFT_3_TM=60.009
PRIMER_RIGHT_3_TM=59.937
PRIMER_LEFT_3_GC_PERCENT=47.826
PRIMER_RIGHT_3_GC_PERCENT=47.826
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_3_MISPRIMING_SCORE=23.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=101
PRIMER_PAIR_PENALTY_4=0.0842
PRIMER_LEFT_4_PENALTY=0.079168
PRIMER_RIGHT_4_PENALTY=0.005048
PRIMER_LEFT_4_SEQUENCE=AGAACATCACACATTCTTCCGTT
PRIMER_RIGHT_4_SEQUENCE=GAGTGGGTTTTTCAGGAGAAGAT
PRIMER_LEFT_4=1676,23
PRIMER_RIGHT_4=2286,23
PRIMER_LEFT_4_TM=59.921
PRIMER_RIGHT_4_TM=59.995
PRIMER_LEFT_4_GC_PERCENT=39.130
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, reverse L1MA5 3'-end of L1 repeat (subfamily L1MA5) - a consensus sequence
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_4_MISPRIMING_SCORE=22.00, MER42a MER42 repetitive sequence: subfamily MER42a - a consensus
PRIMER_LEFT_4_END_STABILITY=9.9000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=611
PRIMER_PAIR_PENALTY_5=0.0867
PRIMER_LEFT_5_PENALTY=0.005048
PRIMER_RIGHT_5_PENALTY=0.081604
PRIMER_LEFT_5_SEQUENCE=ATCTTCTCCTGAAAAACCCACTC
PRIMER_RIGHT_5_SEQUENCE=TGCCAAAAATCTGTACAAGTGTG
PRIMER_LEFT_5=2264,23
PRIMER_RIGHT_5=2986,23
PRIMER_LEFT_5_TM=59.995
PRIMER_RIGHT_5_TM=60.082
PRIMER_LEFT_5_GC_PERCENT=43.478
PRIMER_RIGHT_5_GC_PERCENT=39.130
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=6.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=2.00
PRIMER_LEFT_5_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_5_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_5_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_5_END_STABILITY=6.4000
PRIMER_RIGHT_5_END_STABILITY=6.4000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_5=723
PRIMER_PAIR_PENALTY_6=0.0880
PRIMER_LEFT_6_PENALTY=0.079168
PRIMER_RIGHT_6_PENALTY=0.008825
PRIMER_LEFT_6_SEQUENCE=GACAGTAATGACCTCACGTTTCC
PRIMER_RIGHT_6_SEQUENCE=AGAAGGACAGGGTAATCTTCCAG
PRIMER_LEFT_6=764,23
PRIMER_RIGHT_6=1199,23
PRIMER_LEFT_6_TM=59.921
PRIMER_RIGHT_6_TM=60.009
PRIMER_LEFT_6_GC_PERCENT=47.826
PRIMER_RIGHT_6_GC_PERCENT=47.826
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_MISPRIMING_SCORE=12.00, reverse L1MA2 3'-end of L1 repeat (subfamily L1MA2) - a consensus sequence
PRIMER_RIGHT_6_MISPRIMING_SCORE=12.00, MER43 Homo sapiens clone DOE1-35 MER repetitive sequence - a consensus
PRIMER_PAIR_6_MISPRIMING_SCORE=22.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_6_END_STABILITY=8.5000
PRIMER_RIGHT_6_END_STABILITY=8.2000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_6=436
PRIMER_PAIR_PENALTY_7=0.0880
PRIMER_LEFT_7_PENALTY=0.079168
PRIMER_RIGHT_7_PENALTY=0.008825
PRIMER_LEFT_7_SEQUENCE=GGACAGTAATGACCTCACGTTTC
PRIMER_RIGHT_7_SEQUENCE=AGAAGGACAGGGTAATCTTCCAG
PRIMER_LEFT_7=763,23
PRIMER_RIGHT_7=1199,23
PRIMER_LEFT_7_TM=59.921
PRIMER_RIGHT_7_TM=60.009
PRIMER_LEFT_7_GC_PERCENT=47.826
PRIMER_RIGHT_7_GC_PERCENT=47.826
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=3.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_7_MISPRIMING_SCORE=12.00, MER43 Homo sapiens clone DOE1-35 MER repetitive sequence - a consensus
PRIMER_PAIR_7_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_7_END_STABILITY=6.7000
PRIMER_RIGHT_7_END_STABILITY=8.2000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_7=437
PRIMER_PAIR_PENALTY_8=0.0910
PRIMER_LEFT_8_PENALTY=0.008825
PRIMER_RIGHT_8_PENALTY=0.082173
PRIMER_LEFT_8_SEQUENCE=CTGGAAGATTACCCTGTCCTTCT
PRIMER_RIGHT_8_SEQUENCE=CCATGGAAAGGTATTCATTCGTA
PRIMER_LEFT_8=1177,23
PRIMER_RIGHT_8=1535,23
PRIMER_LEFT_8_TM=60.009
PRIMER_RIGHT_8_TM=60.082
PRIMER_LEFT_8_GC_PERCENT=47.826
PRIMER_RIGHT_8_GC_PERCENT=39.130
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=2.00
PRIMER_LEFT_8_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_8_MISPRIMING_SCORE=11.00, MER42a MER42 repetitive sequence: subfamily MER42a - a consensus
PRIMER_PAIR_8_MISPRIMING_SCORE=21.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_LEFT_8_END_STABILITY=6.7000
PRIMER_RIGHT_8_END_STABILITY=7.4000
PRIMER_PAIR_8_COMPL_ANY=7.00
PRIMER_PAIR_8_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_8=359
PRIMER_PAIR_PENALTY_9=0.0955
PRIMER_LEFT_9_PENALTY=0.079168
PRIMER_RIGHT_9_PENALTY=0.016351
PRIMER_LEFT_9_SEQUENCE=AGAACATCACACATTCTTCCGTT
PRIMER_RIGHT_9_SEQUENCE=TTATTTGCCTTTTCTTTGCCATA
PRIMER_LEFT_9=1676,23
PRIMER_RIGHT_9=1822,23
PRIMER_LEFT_9_TM=59.921
PRIMER_RIGHT_9_TM=59.984
PRIMER_LEFT_9_GC_PERCENT=39.130
PRIMER_RIGHT_9_GC_PERCENT=30.435
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=3.00
PRIMER_LEFT_9_MISPRIMING_SCORE=12.00, reverse L1MA5 3'-end of L1 repeat (subfamily L1MA5) - a consensus sequence
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, Tigger2 Autonomous DNA transposon
PRIMER_PAIR_9_MISPRIMING_SCORE=21.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_9_END_STABILITY=9.9000
PRIMER_RIGHT_9_END_STABILITY=7.4000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_9=147
PRIMER_PAIR_PENALTY_10=0.1018
PRIMER_LEFT_10_PENALTY=0.096730
PRIMER_RIGHT_10_PENALTY=0.005048
PRIMER_LEFT_10_SEQUENCE=GCATGTAAAAATTTGTGGAAAGC
PRIMER_RIGHT_10_SEQUENCE=GAGTGGGTTTTTCAGGAGAAGAT
PRIMER_LEFT_10=1647,23
PRIMER_RIGHT_10=2286,23
PRIMER_LEFT_10_TM=59.903
PRIMER_RIGHT_10_TM=59.995
PRIMER_LEFT_10_GC_PERCENT=34.783
PRIMER_RIGHT_10_GC_PERCENT=43.478
PRIMER_LEFT_10_SELF_ANY=6.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=2.00
PRIMER_RIGHT_10_SELF_END=2.00
PRIMER_LEFT_10_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_10_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_10_MISPRIMING_SCORE=21.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_LEFT_10_END_STABILITY=8.5000
PRIMER_RIGHT_10_END_STABILITY=6.6000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_10=640
PRIMER_PAIR_PENALTY_11=0.1056
PRIMER_LEFT_11_PENALTY=0.008825
PRIMER_RIGHT_11_PENALTY=0.096730
PRIMER_LEFT_11_SEQUENCE=CTGGAAGATTACCCTGTCCTTCT
PRIMER_RIGHT_11_SEQUENCE=GCTTTCCACAAATTTTTACATGC
PRIMER_LEFT_11=1177,23
PRIMER_RIGHT_11=1669,23
PRIMER_LEFT_11_TM=60.009
PRIMER_RIGHT_11_TM=59.903
PRIMER_LEFT_11_GC_PERCENT=47.826
PRIMER_RIGHT_11_GC_PERCENT=34.783
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=6.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=2.00
PRIMER_LEFT_11_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, reverse L1MD2 3'-end of L1 repeat (subfamily L1MD2) - a consensus sequence
PRIMER_PAIR_11_MISPRIMING_SCORE=21.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_LEFT_11_END_STABILITY=6.7000
PRIMER_RIGHT_11_END_STABILITY=8.4000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_11=493
PRIMER_PAIR_PENALTY_12=0.1125
PRIMER_LEFT_12_PENALTY=0.049500
PRIMER_RIGHT_12_PENALTY=0.063026
PRIMER_LEFT_12_SEQUENCE=AGTGACCCCAACAAGTTACAAGA
PRIMER_RIGHT_12_SEQUENCE=GCCTGACAAGTTCTCTGACTGAT
PRIMER_LEFT_12=1104,23
PRIMER_RIGHT_12=1277,23
PRIMER_LEFT_12_TM=59.950
PRIMER_RIGHT_12_TM=59.937
PRIMER_LEFT_12_GC_PERCENT=43.478
PRIMER_RIGHT_12_GC_PERCENT=47.826
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=5.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=2.00
PRIMER_LEFT_12_MISPRIMING_SCORE=12.00, reverse HY3 Ro(hY3) pseudogene - a consensus
PRIMER_RIGHT_12_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_12_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_12_END_STABILITY=7.0000
PRIMER_RIGHT_12_END_STABILITY=6.6000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_12=174
PRIMER_PAIR_PENALTY_13=0.1254
PRIMER_LEFT_13_PENALTY=0.109069
PRIMER_RIGHT_13_PENALTY=0.016351
PRIMER_LEFT_13_SEQUENCE=GTTTACAGTTGGCTGATGATTCC
PRIMER_RIGHT_13_SEQUENCE=TTATTTGCCTTTTCTTTGCCATA
PRIMER_LEFT_13=982,23
PRIMER_RIGHT_13=1822,23
PRIMER_LEFT_13_TM=59.891
PRIMER_RIGHT_13_TM=59.984
PRIMER_LEFT_13_GC_PERCENT=43.478
PRIMER_RIGHT_13_GC_PERCENT=30.435
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=3.00
PRIMER_LEFT_13_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_13_MISPRIMING_SCORE=12.00, Tigger2 Autonomous DNA transposon
PRIMER_PAIR_13_MISPRIMING_SCORE=23.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_13_END_STABILITY=8.1000
PRIMER_RIGHT_13_END_STABILITY=7.4000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_13=841
PRIMER_PAIR_PENALTY_14=0.1254
PRIMER_LEFT_14_PENALTY=0.109069
PRIMER_RIGHT_14_PENALTY=0.016351
PRIMER_LEFT_14_SEQUENCE=GGTTTACAGTTGGCTGATGATTC
PRIMER_RIGHT_14_SEQUENCE=TTATTTGCCTTTTCTTTGCCATA
PRIMER_LEFT_14=981,23
PRIMER_RIGHT_14=1822,23
PRIMER_LEFT_14_TM=59.891
PRIMER_RIGHT_14_TM=59.984
PRIMER_LEFT_14_GC_PERCENT=43.478
PRIMER_RIGHT_14_GC_PERCENT=30.435
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=3.00
PRIMER_RIGHT_14_SELF_END=3.00
PRIMER_LEFT_14_MISPRIMING_SCORE=11.00, MER4C a consensus
PRIMER_RIGHT_14_MISPRIMING_SCORE=12.00, Tigger2 Autonomous DNA transposon
PRIMER_PAIR_14_MISPRIMING_SCORE=22.00, MER4C a consensus
PRIMER_LEFT_14_END_STABILITY=6.6000
PRIMER_RIGHT_14_END_STABILITY=7.4000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_14=842
PRIMER_PAIR_PENALTY_15=0.1287
PRIMER_LEFT_15_PENALTY=0.049500
PRIMER_RIGHT_15_PENALTY=0.079168
PRIMER_LEFT_15_SEQUENCE=AGTGACCCCAACAAGTTACAAGA
PRIMER_RIGHT_15_SEQUENCE=AACGGAAGAATGTGTGATGTTCT
PRIMER_LEFT_15=1104,23
PRIMER_RIGHT_15=1698,23
PRIMER_LEFT_15_TM=59.950
PRIMER_RIGHT_15_TM=59.921
PRIMER_LEFT_15_GC_PERCENT=43.478
PRIMER_RIGHT_15_GC_PERCENT=39.130
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=3.00
PRIMER_LEFT_15_MISPRIMING_SCORE=12.00, reverse HY3 Ro(hY3) pseudogene - a consensus
PRIMER_RIGHT_15_MISPRIMING_SCORE=12.00, MER42a MER42 repetitive sequence: subfamily MER42a - a consensus
PRIMER_PAIR_15_MISPRIMING_SCORE=21.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_15_END_STABILITY=7.0000
PRIMER_RIGHT_15_END_STABILITY=6.4000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_15=595
PRIMER_PAIR_PENALTY_16=0.1288
PRIMER_LEFT_16_PENALTY=0.112479
PRIMER_RIGHT_16_PENALTY=0.016351
PRIMER_LEFT_16_SEQUENCE=ACTGGAAGATTACCCTGTCCTTC
PRIMER_RIGHT_16_SEQUENCE=TTATTTGCCTTTTCTTTGCCATA
PRIMER_LEFT_16=1176,23
PRIMER_RIGHT_16=1822,23
PRIMER_LEFT_16_TM=59.888
PRIMER_RIGHT_16_TM=59.984
PRIMER_LEFT_16_GC_PERCENT=47.826
PRIMER_RIGHT_16_GC_PERCENT=30.435
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=3.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=3.00
PRIMER_LEFT_16_MISPRIMING_SCORE=11.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_16_MISPRIMING_SCORE=12.00, Tigger2 Autonomous DNA transposon
PRIMER_PAIR_16_MISPRIMING_SCORE=22.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_16_END_STABILITY=8.2000
PRIMER_RIGHT_16_END_STABILITY=7.4000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_16=647
PRIMER_PAIR_PENALTY_17=0.1303
PRIMER_LEFT_17_PENALTY=0.079168
PRIMER_RIGHT_17_PENALTY=0.051149
PRIMER_LEFT_17_SEQUENCE=AGAACATCACACATTCTTCCGTT
PRIMER_RIGHT_17_SEQUENCE=CTTTGACTGTTTGGGTGGTAAAG
PRIMER_LEFT_17=1676,23
PRIMER_RIGHT_17=2183,23
PRIMER_LEFT_17_TM=59.921
PRIMER_RIGHT_17_TM=59.949
PRIMER_LEFT_17_GC_PERCENT=39.130
PRIMER_RIGHT_17_GC_PERCENT=43.478
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_MISPRIMING_SCORE=12.00, reverse L1MA5 3'-end of L1 repeat (subfamily L1MA5) - a consensus sequence
PRIMER_RIGHT_17_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_17_MISPRIMING_SCORE=21.00, MER21B a consensus
PRIMER_LEFT_17_END_STABILITY=9.9000
PRIMER_RIGHT_17_END_STABILITY=6.3000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_17=508
PRIMER_PAIR_PENALTY_18=0.1317
PRIMER_LEFT_18_PENALTY=0.049500
PRIMER_RIGHT_18_PENALTY=0.082173
PRIMER_LEFT_18_SEQUENCE=AGTGACCCCAACAAGTTACAAGA
PRIMER_RIGHT_18_SEQUENCE=CCATGGAAAGGTATTCATTCGTA
PRIMER_LEFT_18=1104,23
PRIMER_RIGHT_18=1535,23
PRIMER_LEFT_18_TM=59.950
PRIMER_RIGHT_18_TM=60.082
PRIMER_LEFT_18_GC_PERCENT=43.478
PRIMER_RIGHT_18_GC_PERCENT=39.130
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=6.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=2.00
PRIMER_LEFT_18_MISPRIMING_SCORE=12.00, reverse HY3 Ro(hY3) pseudogene - a consensus
PRIMER_RIGHT_18_MISPRIMING_SCORE=11.00, MER42a MER42 repetitive sequence: subfamily MER42a - a consensus
PRIMER_PAIR_18_MISPRIMING_SCORE=21.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_18_END_STABILITY=7.0000
PRIMER_RIGHT_18_END_STABILITY=7.4000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_18=432
PRIMER_PAIR_PENALTY_19=0.1328
PRIMER_LEFT_19_PENALTY=0.051149
PRIMER_RIGHT_19_PENALTY=0.081604
PRIMER_LEFT_19_SEQUENCE=CTTTACCACCCAAACAGTCAAAG
PRIMER_RIGHT_19_SEQUENCE=TGCCAAAAATCTGTACAAGTGTG
PRIMER_LEFT_19=2161,23
PRIMER_RIGHT_19=2986,23
PRIMER_LEFT_19_TM=59.949
PRIMER_RIGHT_19_TM=60.082
PRIMER_LEFT_19_GC_PERCENT=43.478
PRIMER_RIGHT_19_GC_PERCENT=39.130
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=2.00
PRIMER_LEFT_19_MISPRIMING_SCORE=11.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_19_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_19_MISPRIMING_SCORE=21.00, MER42a MER42 repetitive sequence: subfamily MER42a - a consensus
PRIMER_LEFT_19_END_STABILITY=7.3000
PRIMER_RIGHT_19_END_STABILITY=6.4000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_19=826
=