PRIMER_SEQUENCE_ID=NM_002831
SEQUENCE=GCCGGCTGCCCCAGGCCAGTGGAGTGGCAGCCCCAGAACTGGGACCACCGGGGGTGGTGAGGCGGCCCGGCACTGGGAGCTGCATCTGAGGCTTAGTCCCTGAGCTCTCTGCCTGCCCAGACTAGCTGCACCTCCTCATTCCCTGCGCCCCCTTCCTCTCCGGAAGCCCCCAGGATGGTGAGGTGGTTTCACCGAGACCTCAGTGGGCTGGATGCAGAGACCCTGCTCAAGGGCCGAGGTGTCCACGGTAGCTTCCTGGCTCGGCCCAGTCGCAAGAACCAGGGTGACTTCTCGCTCTCCGTCAGGGTGGGGGATCAGGTGACCCATATTCGGATCCAGAACTCAGGGGATTTCTATGACCTGTATGGAGGGGAGAAGTTTGCGACTCTGACAGAGCTGGTGGAGTACTACACTCAGCAGCAGGGTGTCCTGCAGGACCGCGACGGCACCATCATCCACCTCAAGTACCCGCTGAACTGCTCCGATCCCACTAGTGAGAGGTGGTACCATGGCCACATGTCTGGCGGGCAGGCAGAGACGCTGCTGCAGGCCAAGGGCGAGCCCTGGACGTTTCTTGTGCGTGAGAGCCTCAGCCAGCCTGGAGACTTCGTGCTTTCTGTGCTCAGTGACCAGCCCAAGGCTGGCCCAGGCTCCCCGCTCAGGGTCACCCACATCAAGGTCATGTGCGAGGGTGGACGCTACACAGTGGGTGGTTTGGAGACCTTCGACAGCCTCACGGACCTGGTGGAGCATTTCAAGAAGACGGGGATTGAGGAGGCCTCAGGCGCCTTTGTCTACCTGCGGCAGCCGTACTATGCCACGAGGGTGAATGCGGCTGACATTGAGAACCGAGTGTTGGAACTGAACAAGAAGCAGGAGTCCGAGGATACAGCCAAGGCTGGCTTCTGGGAGGAGTTTGAGAGTTTGCAGAAGCAGGAGGTGAAGAACTTGCACCAGCGTCTGGAAGGGCAGCGGCCAGAGAACAAGGGCAAGAACCGCTACAAGAACATTCTCCCCTTTGACCACAGCCGAGTGATCCTGCAGGGACGGGACAGTAACATCCCCGGGTCCGACTACATCAATGCCAACTACATCAAGAACCAGCTGCTAGGCCCTGATGAGAACGCTAAGACCTACATCGCCAGCCAGGGCTGTCTGGAGGCCACGGTCAATGACTTCTGGCAGATGGCGTGGCAGGAGAACAGCCGTGTCATCGTCATGACCACCCGAGAGGTGGAGAAAGGCCGGAACAAATGCGTCCCATACTGGCCCGAGGTGGGCATGCAGCGTGCTTATGGGCCCTACTCTGTGACCAACTGCGGGGAGCATGACACAACCGAATACAAACTCCGTACCTTACAGGTCTCCCCGCTGGACAATGGAGACCTGATTCGGGAGATCTGGCATTACCAGTACCTGAGCTGGCCCGACCATGGGGTCCCCAGTGAGCCTGGGGGTGTCCTCAGCTTCCTGGACCAGATCAACCAGCGGCAGGAAAGTCTGCCTCACGCAGGGCCCATCATCGTGCACTGCAGCGCCGGCATCGGCCGCACAGGCACCATCATTGTCATCGACATGCTCATGGAGAACATCTCCACCAAGGGCCTGGACTGTGACATTGACATCCAGAAGACCATCCAGATGGTGCGGGCGCAGCGCTCGGGCATGGTGCAGACGGAGGCGCAGTACAAGTTCATCTACGTGGCCATCGCCCAGTTCATTGAAACCACTAAGAAGAAGCTGGAGGTCCTGCAGTCGCAGAAGGGCCAGGAGTCGGAGTACGGGAACATCACCTATCCCCCAGCCATGAAGAATGCCCATGCCAAGGCCTCCCGCACCTCGTCCAAACACAAGGAGGATGTGTATGAGAACCTGCACACTAAGAACAAGAGGGAGGAGAAAGTGAAGAAGCAGCGGTCAGCAGACAAGGAGAAGAGCAAGGGTTCCCTCAAGAGGAAGTGAGCGGTGCTGTCCTCAGGTGGCCATGCCTCAGCCCTGACCCTGTGGAAGCATTTCGCGATGGACAGACTCACAACCTGAACCTAGGAGTGCCCCATTCTTTTGTAATTTAAATGGCTGCATCCCCCCCACCTCTCCCTGACCCTGTATATAGCCCAGCCAGGCCCCAGGCAGGGCCAACCCTTCTCCTCTTGTAAATAAAGCCCTGGGATCACTGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 25672, GC content failed 367, low tm 6410, high tm 13838, high any compl 13, high end compl 28, high repeat similarity 41, long poly-x seq 22, ok 4953
PRIMER_RIGHT_EXPLAIN=considered 25562, GC content failed 934, low tm 6378, high tm 13269, high end compl 3, high repeat similarity 7, long poly-x seq 38, ok 4933
PRIMER_PAIR_EXPLAIN=considered 688, unacceptable product size 604, high end compl 8, ok 76
PRIMER_PAIR_PENALTY=0.0749
PRIMER_LEFT_PENALTY=0.017590
PRIMER_RIGHT_PENALTY=0.057286
PRIMER_LEFT_SEQUENCE=GCTACAAGAACATTCTCCCCTTT
PRIMER_RIGHT_SEQUENCE=GGAGACCTGTAAGGTACGGAGTT
PRIMER_LEFT=997,23
PRIMER_RIGHT=1367,23
PRIMER_LEFT_TM=60.018
PRIMER_RIGHT_TM=59.943
PRIMER_LEFT_GC_PERCENT=43.478
PRIMER_RIGHT_GC_PERCENT=52.174
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_MISPRIMING_SCORE=11.00, LTR7 LTR from human endogenous retrovirus RTVL-H2
PRIMER_RIGHT_MISPRIMING_SCORE=9.00, L1MB7 3'-end of L1 repeat (subfamily L1MB7) - a consensus sequence
PRIMER_PAIR_MISPRIMING_SCORE=20.00, reverse L1MB7 3'-end of L1 repeat (subfamily L1MB7) - a consensus sequence
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=371
PRIMER_PAIR_PENALTY_1=0.1035
PRIMER_LEFT_1_PENALTY=0.017590
PRIMER_RIGHT_1_PENALTY=0.085878
PRIMER_LEFT_1_SEQUENCE=GCTACAAGAACATTCTCCCCTTT
PRIMER_RIGHT_1_SEQUENCE=CTTAGTGGTTTCAATGAACTGGG
PRIMER_LEFT_1=997,23
PRIMER_RIGHT_1=1733,23
PRIMER_LEFT_1_TM=60.018
PRIMER_RIGHT_1_TM=59.914
PRIMER_LEFT_1_GC_PERCENT=43.478
PRIMER_RIGHT_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_MISPRIMING_SCORE=11.00, LTR7 LTR from human endogenous retrovirus RTVL-H2
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_1_MISPRIMING_SCORE=23.00, reverse L1MB7 3'-end of L1 repeat (subfamily L1MB7) - a consensus sequence
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=737
PRIMER_PAIR_PENALTY_2=0.1135
PRIMER_LEFT_2_PENALTY=0.027592
PRIMER_RIGHT_2_PENALTY=0.085878
PRIMER_LEFT_2_SEQUENCE=TGTGACATTGACATCCAGAAGAC
PRIMER_RIGHT_2_SEQUENCE=CTTAGTGGTTTCAATGAACTGGG
PRIMER_LEFT_2=1611,23
PRIMER_RIGHT_2=1733,23
PRIMER_LEFT_2_TM=60.028
PRIMER_RIGHT_2_TM=59.914
PRIMER_LEFT_2_GC_PERCENT=43.478
PRIMER_RIGHT_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_2_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_2_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=123
PRIMER_PAIR_PENALTY_3=0.1135
PRIMER_LEFT_3_PENALTY=0.027592
PRIMER_RIGHT_3_PENALTY=0.085878
PRIMER_LEFT_3_SEQUENCE=GACTGTGACATTGACATCCAGAA
PRIMER_RIGHT_3_SEQUENCE=CTTAGTGGTTTCAATGAACTGGG
PRIMER_LEFT_3=1608,23
PRIMER_RIGHT_3=1733,23
PRIMER_LEFT_3_TM=60.028
PRIMER_RIGHT_3_TM=59.914
PRIMER_LEFT_3_GC_PERCENT=43.478
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_3_MISPRIMING_SCORE=24.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=126
PRIMER_PAIR_PENALTY_4=0.1383
PRIMER_LEFT_4_PENALTY=0.017590
PRIMER_RIGHT_4_PENALTY=0.120688
PRIMER_LEFT_4_SEQUENCE=GCTACAAGAACATTCTCCCCTTT
PRIMER_RIGHT_4_SEQUENCE=CACGTAGATGAACTTGTACTGCG
PRIMER_LEFT_4=997,23
PRIMER_RIGHT_4=1703,23
PRIMER_LEFT_4_TM=60.018
PRIMER_RIGHT_4_TM=59.879
PRIMER_LEFT_4_GC_PERCENT=43.478
PRIMER_RIGHT_4_GC_PERCENT=47.826
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_MISPRIMING_SCORE=11.00, LTR7 LTR from human endogenous retrovirus RTVL-H2
PRIMER_RIGHT_4_MISPRIMING_SCORE=11.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_4_MISPRIMING_SCORE=20.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=10.2000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=707
PRIMER_PAIR_PENALTY_5=0.1395
PRIMER_LEFT_5_PENALTY=0.027592
PRIMER_RIGHT_5_PENALTY=0.111908
PRIMER_LEFT_5_SEQUENCE=GACTGTGACATTGACATCCAGAA
PRIMER_RIGHT_5_SEQUENCE=ATTTACAAGAGGAGAAGGGTTGG
PRIMER_LEFT_5=1608,23
PRIMER_RIGHT_5=2157,23
PRIMER_LEFT_5_TM=60.028
PRIMER_RIGHT_5_TM=59.888
PRIMER_LEFT_5_GC_PERCENT=43.478
PRIMER_RIGHT_5_GC_PERCENT=43.478
PRIMER_LEFT_5_SELF_ANY=5.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_5_MISPRIMING_SCORE=12.00, MER21B a consensus
PRIMER_PAIR_5_MISPRIMING_SCORE=22.00, reverse L1MA9 3'-end of L1 repeat (subfamily L1MA9) - a consensus sequence
PRIMER_LEFT_5_END_STABILITY=7.0000
PRIMER_RIGHT_5_END_STABILITY=8.2000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_5=550
PRIMER_PAIR_PENALTY_6=0.1395
PRIMER_LEFT_6_PENALTY=0.027592
PRIMER_RIGHT_6_PENALTY=0.111908
PRIMER_LEFT_6_SEQUENCE=TGTGACATTGACATCCAGAAGAC
PRIMER_RIGHT_6_SEQUENCE=ATTTACAAGAGGAGAAGGGTTGG
PRIMER_LEFT_6=1611,23
PRIMER_RIGHT_6=2157,23
PRIMER_LEFT_6_TM=60.028
PRIMER_RIGHT_6_TM=59.888
PRIMER_LEFT_6_GC_PERCENT=43.478
PRIMER_RIGHT_6_GC_PERCENT=43.478
PRIMER_LEFT_6_SELF_ANY=5.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=2.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_6_MISPRIMING_SCORE=12.00, MER21B a consensus
PRIMER_PAIR_6_MISPRIMING_SCORE=22.00, MER21B a consensus
PRIMER_LEFT_6_END_STABILITY=6.4000
PRIMER_RIGHT_6_END_STABILITY=8.2000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_6=547
PRIMER_PAIR_PENALTY_7=0.1432
PRIMER_LEFT_7_PENALTY=0.057286
PRIMER_RIGHT_7_PENALTY=0.085878
PRIMER_LEFT_7_SEQUENCE=AACTCCGTACCTTACAGGTCTCC
PRIMER_RIGHT_7_SEQUENCE=CTTAGTGGTTTCAATGAACTGGG
PRIMER_LEFT_7=1345,23
PRIMER_RIGHT_7=1733,23
PRIMER_LEFT_7_TM=59.943
PRIMER_RIGHT_7_TM=59.914
PRIMER_LEFT_7_GC_PERCENT=52.174
PRIMER_RIGHT_7_GC_PERCENT=43.478
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=7.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_MISPRIMING_SCORE=11.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_7_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_7_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_7_END_STABILITY=7.9000
PRIMER_RIGHT_7_END_STABILITY=9.7000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_7=389
PRIMER_PAIR_PENALTY_8=0.1567
PRIMER_LEFT_8_PENALTY=0.017590
PRIMER_RIGHT_8_PENALTY=0.139070
PRIMER_LEFT_8_SEQUENCE=GCTACAAGAACATTCTCCCCTTT
PRIMER_RIGHT_8_SEQUENCE=CCTCTTGTTCTTAGTGTGCAGGT
PRIMER_LEFT_8=997,23
PRIMER_RIGHT_8=1892,23
PRIMER_LEFT_8_TM=60.018
PRIMER_RIGHT_8_TM=59.861
PRIMER_LEFT_8_GC_PERCENT=43.478
PRIMER_RIGHT_8_GC_PERCENT=47.826
PRIMER_LEFT_8_SELF_ANY=6.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_MISPRIMING_SCORE=11.00, LTR7 LTR from human endogenous retrovirus RTVL-H2
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_8_MISPRIMING_SCORE=21.00, reverse MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_LEFT_8_END_STABILITY=8.5000
PRIMER_RIGHT_8_END_STABILITY=7.9000
PRIMER_PAIR_8_COMPL_ANY=7.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_8=896
PRIMER_PAIR_PENALTY_9=0.1581
PRIMER_LEFT_9_PENALTY=0.017590
PRIMER_RIGHT_9_PENALTY=0.140508
PRIMER_LEFT_9_SEQUENCE=GCTACAAGAACATTCTCCCCTTT
PRIMER_RIGHT_9_SEQUENCE=GTAGATGAACTTGTACTGCGCCT
PRIMER_LEFT_9=997,23
PRIMER_RIGHT_9=1700,23
PRIMER_LEFT_9_TM=60.018
PRIMER_RIGHT_9_TM=59.859
PRIMER_LEFT_9_GC_PERCENT=43.478
PRIMER_RIGHT_9_GC_PERCENT=47.826
PRIMER_LEFT_9_SELF_ANY=6.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_MISPRIMING_SCORE=11.00, LTR7 LTR from human endogenous retrovirus RTVL-H2
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_9_MISPRIMING_SCORE=20.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_9_END_STABILITY=8.5000
PRIMER_RIGHT_9_END_STABILITY=11.4000
PRIMER_PAIR_9_COMPL_ANY=7.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_9=704
PRIMER_PAIR_PENALTY_10=0.1667
PRIMER_LEFT_10_PENALTY=0.027592
PRIMER_RIGHT_10_PENALTY=0.139070
PRIMER_LEFT_10_SEQUENCE=TGTGACATTGACATCCAGAAGAC
PRIMER_RIGHT_10_SEQUENCE=CCTCTTGTTCTTAGTGTGCAGGT
PRIMER_LEFT_10=1611,23
PRIMER_RIGHT_10=1892,23
PRIMER_LEFT_10_TM=60.028
PRIMER_RIGHT_10_TM=59.861
PRIMER_LEFT_10_GC_PERCENT=43.478
PRIMER_RIGHT_10_GC_PERCENT=47.826
PRIMER_LEFT_10_SELF_ANY=5.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=2.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_10_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_10_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_10_END_STABILITY=6.4000
PRIMER_RIGHT_10_END_STABILITY=7.9000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_10=282
PRIMER_PAIR_PENALTY_11=0.1692
PRIMER_LEFT_11_PENALTY=0.057286
PRIMER_RIGHT_11_PENALTY=0.111908
PRIMER_LEFT_11_SEQUENCE=AACTCCGTACCTTACAGGTCTCC
PRIMER_RIGHT_11_SEQUENCE=ATTTACAAGAGGAGAAGGGTTGG
PRIMER_LEFT_11=1345,23
PRIMER_RIGHT_11=2157,23
PRIMER_LEFT_11_TM=59.943
PRIMER_RIGHT_11_TM=59.888
PRIMER_LEFT_11_GC_PERCENT=52.174
PRIMER_RIGHT_11_GC_PERCENT=43.478
PRIMER_LEFT_11_SELF_ANY=6.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_MISPRIMING_SCORE=11.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, MER21B a consensus
PRIMER_PAIR_11_MISPRIMING_SCORE=22.00, MER21B a consensus
PRIMER_LEFT_11_END_STABILITY=7.9000
PRIMER_RIGHT_11_END_STABILITY=8.2000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_11=813
PRIMER_PAIR_PENALTY_12=0.1754
PRIMER_LEFT_12_PENALTY=0.017590
PRIMER_RIGHT_12_PENALTY=0.157853
PRIMER_LEFT_12_SEQUENCE=GCTACAAGAACATTCTCCCCTTT
PRIMER_RIGHT_12_SEQUENCE=ATACACATCCTCCTTGTGTTTGG
PRIMER_LEFT_12=997,23
PRIMER_RIGHT_12=1865,23
PRIMER_LEFT_12_TM=60.018
PRIMER_RIGHT_12_TM=60.158
PRIMER_LEFT_12_GC_PERCENT=43.478
PRIMER_RIGHT_12_GC_PERCENT=43.478
PRIMER_LEFT_12_SELF_ANY=6.00
PRIMER_RIGHT_12_SELF_ANY=5.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_MISPRIMING_SCORE=11.00, LTR7 LTR from human endogenous retrovirus RTVL-H2
PRIMER_RIGHT_12_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_12_MISPRIMING_SCORE=19.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_12_END_STABILITY=8.5000
PRIMER_RIGHT_12_END_STABILITY=8.8000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_12=869
PRIMER_PAIR_PENALTY_13=0.1780
PRIMER_LEFT_13_PENALTY=0.057286
PRIMER_RIGHT_13_PENALTY=0.120688
PRIMER_LEFT_13_SEQUENCE=AACTCCGTACCTTACAGGTCTCC
PRIMER_RIGHT_13_SEQUENCE=CACGTAGATGAACTTGTACTGCG
PRIMER_LEFT_13=1345,23
PRIMER_RIGHT_13=1703,23
PRIMER_LEFT_13_TM=59.943
PRIMER_RIGHT_13_TM=59.879
PRIMER_LEFT_13_GC_PERCENT=52.174
PRIMER_RIGHT_13_GC_PERCENT=47.826
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=3.00
PRIMER_LEFT_13_MISPRIMING_SCORE=11.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_13_MISPRIMING_SCORE=11.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_13_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_13_END_STABILITY=7.9000
PRIMER_RIGHT_13_END_STABILITY=10.2000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_13=359
PRIMER_PAIR_PENALTY_14=0.1812
PRIMER_LEFT_14_PENALTY=0.017590
PRIMER_RIGHT_14_PENALTY=0.163603
PRIMER_LEFT_14_SEQUENCE=GCTACAAGAACATTCTCCCCTTT
PRIMER_RIGHT_14_SEQUENCE=TCTTCTGGATGTCAATGTCACAG
PRIMER_LEFT_14=997,23
PRIMER_RIGHT_14=1632,23
PRIMER_LEFT_14_TM=60.018
PRIMER_RIGHT_14_TM=60.164
PRIMER_LEFT_14_GC_PERCENT=43.478
PRIMER_RIGHT_14_GC_PERCENT=43.478
PRIMER_LEFT_14_SELF_ANY=6.00
PRIMER_RIGHT_14_SELF_ANY=5.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=3.00
PRIMER_LEFT_14_MISPRIMING_SCORE=11.00, LTR7 LTR from human endogenous retrovirus RTVL-H2
PRIMER_RIGHT_14_MISPRIMING_SCORE=12.00, reverse L1MA9 3'-end of L1 repeat (subfamily L1MA9) - a consensus sequence
PRIMER_PAIR_14_MISPRIMING_SCORE=21.00, LTR7 LTR from human endogenous retrovirus RTVL-H2
PRIMER_LEFT_14_END_STABILITY=8.5000
PRIMER_RIGHT_14_END_STABILITY=6.7000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_14=636
PRIMER_PAIR_PENALTY_15=0.1854
PRIMER_LEFT_15_PENALTY=0.027592
PRIMER_RIGHT_15_PENALTY=0.157853
PRIMER_LEFT_15_SEQUENCE=GACTGTGACATTGACATCCAGAA
PRIMER_RIGHT_15_SEQUENCE=ATACACATCCTCCTTGTGTTTGG
PRIMER_LEFT_15=1608,23
PRIMER_RIGHT_15=1865,23
PRIMER_LEFT_15_TM=60.028
PRIMER_RIGHT_15_TM=60.158
PRIMER_LEFT_15_GC_PERCENT=43.478
PRIMER_RIGHT_15_GC_PERCENT=43.478
PRIMER_LEFT_15_SELF_ANY=5.00
PRIMER_RIGHT_15_SELF_ANY=5.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_15_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_15_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_15_END_STABILITY=7.0000
PRIMER_RIGHT_15_END_STABILITY=8.8000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_15=258
PRIMER_PAIR_PENALTY_16=0.1896
PRIMER_LEFT_16_PENALTY=0.017590
PRIMER_RIGHT_16_PENALTY=0.172019
PRIMER_LEFT_16_SEQUENCE=GCTACAAGAACATTCTCCCCTTT
PRIMER_RIGHT_16_SEQUENCE=AGGTACGGAGTTTGTATTCGGTT
PRIMER_LEFT_16=997,23
PRIMER_RIGHT_16=1356,23
PRIMER_LEFT_16_TM=60.018
PRIMER_RIGHT_16_TM=60.172
PRIMER_LEFT_16_GC_PERCENT=43.478
PRIMER_RIGHT_16_GC_PERCENT=43.478
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_MISPRIMING_SCORE=11.00, LTR7 LTR from human endogenous retrovirus RTVL-H2
PRIMER_RIGHT_16_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_16_MISPRIMING_SCORE=21.00, reverse L1MB7 3'-end of L1 repeat (subfamily L1MB7) - a consensus sequence
PRIMER_LEFT_16_END_STABILITY=8.5000
PRIMER_RIGHT_16_END_STABILITY=9.9000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_16=360
PRIMER_PAIR_PENALTY_17=0.1896
PRIMER_LEFT_17_PENALTY=0.017590
PRIMER_RIGHT_17_PENALTY=0.172019
PRIMER_LEFT_17_SEQUENCE=GCTACAAGAACATTCTCCCCTTT
PRIMER_RIGHT_17_SEQUENCE=AAGGTACGGAGTTTGTATTCGGT
PRIMER_LEFT_17=997,23
PRIMER_RIGHT_17=1357,23
PRIMER_LEFT_17_TM=60.018
PRIMER_RIGHT_17_TM=60.172
PRIMER_LEFT_17_GC_PERCENT=43.478
PRIMER_RIGHT_17_GC_PERCENT=43.478
PRIMER_LEFT_17_SELF_ANY=6.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=2.00
PRIMER_LEFT_17_MISPRIMING_SCORE=11.00, LTR7 LTR from human endogenous retrovirus RTVL-H2
PRIMER_RIGHT_17_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_17_MISPRIMING_SCORE=22.00, reverse L1MB7 3'-end of L1 repeat (subfamily L1MB7) - a consensus sequence
PRIMER_LEFT_17_END_STABILITY=8.5000
PRIMER_RIGHT_17_END_STABILITY=9.6000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_17=361
PRIMER_PAIR_PENALTY_18=0.1964
PRIMER_LEFT_18_PENALTY=0.057286
PRIMER_RIGHT_18_PENALTY=0.139070
PRIMER_LEFT_18_SEQUENCE=AACTCCGTACCTTACAGGTCTCC
PRIMER_RIGHT_18_SEQUENCE=CCTCTTGTTCTTAGTGTGCAGGT
PRIMER_LEFT_18=1345,23
PRIMER_RIGHT_18=1892,23
PRIMER_LEFT_18_TM=59.943
PRIMER_RIGHT_18_TM=59.861
PRIMER_LEFT_18_GC_PERCENT=52.174
PRIMER_RIGHT_18_GC_PERCENT=47.826
PRIMER_LEFT_18_SELF_ANY=6.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_MISPRIMING_SCORE=11.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_18_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_18_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_18_END_STABILITY=7.9000
PRIMER_RIGHT_18_END_STABILITY=7.9000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_18=548
PRIMER_PAIR_PENALTY_19=0.1978
PRIMER_LEFT_19_PENALTY=0.057286
PRIMER_RIGHT_19_PENALTY=0.140508
PRIMER_LEFT_19_SEQUENCE=AACTCCGTACCTTACAGGTCTCC
PRIMER_RIGHT_19_SEQUENCE=GTAGATGAACTTGTACTGCGCCT
PRIMER_LEFT_19=1345,23
PRIMER_RIGHT_19=1700,23
PRIMER_LEFT_19_TM=59.943
PRIMER_RIGHT_19_TM=59.859
PRIMER_LEFT_19_GC_PERCENT=52.174
PRIMER_RIGHT_19_GC_PERCENT=47.826
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_MISPRIMING_SCORE=11.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_19_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_19_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_19_END_STABILITY=7.9000
PRIMER_RIGHT_19_END_STABILITY=11.4000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_19=356
=