PRIMER_SEQUENCE_ID=NM_002832
SEQUENCE=GCCTTCTCAGCCCTCAAAGACAGACCGACAGACAGACAGACAGCTGGCAAGAGGCAGCCTGGGGGCCACAGCTGCTTCAGCAGACCTCATGGCTGAGTGAGCCTCCCCTGGGCCCAGCACCCCACCTCAGCATGGTCCAAGCCCATGGGGGGCGCTCCAGAGCACAGCCGTTGACCTTGTCTTTGGGGGCAGCCATGACCCAGCCTCCGCCTGAAAAAACGCCAGCCAAGAAGCATGTGCGACTGCAGGAGAGGCGGGGCTCCAATGTGGCTCTGATGCTGGACGTTCGGTCCCTGGGGGCCGTAGAACCCATCTGCTCTGTGAACACACCCCGGGAGGTCACCCTACACTTTCTGCGCACTGCTGGACACCCCCTTACCCGCTGGGCCCTTCAGCGCCAGCCACCCAGCCCCAAGCAACTGGAAGAAGAATTCTTGAAGATCCCTTCAAACTTTGTCAGCCCCGAAGACCTGGACATCCCTGGCCACGCCTCCAAGGACCGATACAAGACCATCTTGCCAAATCCCCAGAGCCGTGTCTGTCTAGGCCGGGCACAGAGCCAGGAGGACGGAGATTACATCAATGCCAACTACATCCGAGGCTATGACGGGAAGGAGAAGGTCTACATTGCCACCCAGGGCCCCATGCCCAACACTGTGTCGGACTTCTGGGAGATGGTGTGGCAAGAGGAAGTGTCCCTCATTGTCATGCTCACTCAGCTCCGAGAGGGCAAGGAGAAATGTGTCCACTACTGGCCCACAGAAGAGGAAACCTATGGACCCTTCCAGATCCGCATCCAGGACATGAAAGAGTGCCCAGAATACACTGTGCGGCAGCTCACCATCCAGTACCAGGAAGAGCGCCGGTCAGTAAAGCACATCCTCTTTTCGGCCTGGCCAGACCATCAGACACCAGAATCAGCTGGGCCCCTGCTGCGCCTAGTGGCAGAGGTGGAGGAGAGCCCGGAGACAGCCGCCCACCCCGGGCCTATCGTAGTCCACTGCAGTGCAGGGATTGGCCGGACGGGCTGCTTCATCGCCACGCGAATTGGCTGTCAACAGCTGAAAGCCCGAGGAGAAGTGGACATTCTGGGTATTGTGTGCCAACTGCGGCTAGACAGAGGGGGGATGATCCAGACGGCAGAGCAGTACCAGTTCCTGCACCACACTTTGGCCCTGTATGCAGGCCAGCTGCCTGAGGAACCCAGCCCCTGACCCCTGCCACCCTCCGGTGGCCCAGGTGCCTACCTCCCTCAAGCCTGGGAAGGTGGGTCTGGGGAAAGTGGGCCGAGTGATCTGGGGGTACCCCTTGGGTTGGTGTGGGGAAGGAGTGCCTCCTTAGTGGTGCTTGCAGTCACAGGAAGCAGCAGCAGTAAGGACAAGGGGCCGGATTCCAGGTCTTCAACACTGGCCACTCCTCTGCTTCCTCTGTTGGCCCCAGATGGACAGTAAGGGGAACCTCCAATGTCTCTCTGAACTTAAAGACAGGAGCTGGCATTTATGACAGACAAAGAAAGAAGCCCAGGTGTCCTGGTGTTCTCTGAGACACTCTTTGTGAGCTTCAGTTTCCTGTTCTATAACATGAACATAAGTGCTTAGCTGCCATGAGGGAAAAGTAATGAGAGAAGTTTCTAGAAGCCACTCCAGCCACTCCTTCCTGGGGCTGACAAAAGGGTGATTCCAAGATCATCCTTCACCCGAGGTCCTGCCCAAGCACAGGCCAGATGCAAGAATGGGGAAAAGTCTGGTCCTGATCTCCAAGTCTCAACATCCTATCAGTGACTCTGCTCCCTGACCACACATCGGAAGGGCTGGATGACCCCAATCAAAAGAAAGAACAAGGACTCTGGTTACCCTTGCCTCCACCCATGTGTCATAAGAGTAGGCTACAGAGGTGACCAGGCCTGGCAGTTGAAATCTCTGGAAGAGGGAACATGTGGGGACTACTCAGAGGCAAAGAGGAGCTGCTCCTGCCTCCATGGTTGCTGGCCACTCCCACCAACTACTCTTAGGGAGGCTAAGCAGTCTCTGTTTTGCTTCCATGGCTCAAATAATACCCTGGGTATGCAGGACCCACTATACCTTGCATTTGCTGAGTACACCTAGAGAGCTTGGCTGTTTCCAAAAACAATCAGGGTCATAACCATCCATGCAGACATGGAGGCTCGGCTGAACCAGGACTCCTCACTGTCTACCTGAGAGAATGAGCACCCCTCATCCATCTCAGCATCAACACAATTTCCAGGGGACCTCAGGTCTACCTCAGGACTGAACGCCACACCTCAGGATTCCTCCTCCTTGAATCTGAGACTGGCTGCCCATTCTGAGATGGGGATGAAGGTAAGATGCCGCATCACCAGGCACGCCGCCCCTGACAGCTGCCTTGATACCAGCTCTCTGTGGAAACCCCCGAGGAGTTGGATCTGGAGAACAGCTGGGCCTCCTCACTCAGGACTTCTCTCCTGAAGAACACGCAGTGCTAAAACTGAGGATGATTTCCCTAATGCTTCTGCTTGGCCTTATGGAGGAGCTGCTCCTTCCTTACAGCCTTGGGGATGGACTTGCCCACACCTCCACCTCCCCTGAGCCCTGTGAGAGGCACGACTGTCTATGCCAATGAGGCTCGGTGGGGGGCTCTCAAGTGCCTGATCCTGCCCTGGGCTCAGAGCCAGCCCAGAGGGAAGCAACTGCACAGCCCCACAGGCCCTCCCTGGCACTGTCCCCCCAACCCCATCTCAGAGCTCAGAGGGTACAAGCTCCAGAACAGTAACCAAGTGGGAAAATAAAGACTTCTTGGATGACTGAC
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 31798, GC content failed 230, low tm 9354, high tm 15313, high any compl 10, high end compl 26, high repeat similarity 48, long poly-x seq 91, ok 6726
PRIMER_RIGHT_EXPLAIN=considered 31656, GC content failed 225, low tm 9552, high tm 14939, high any compl 4, high end compl 9, high repeat similarity 11, long poly-x seq 105, ok 6811
PRIMER_PAIR_EXPLAIN=considered 343, unacceptable product size 301, high end compl 3, ok 39
PRIMER_PAIR_PENALTY=0.0653
PRIMER_LEFT_PENALTY=0.063381
PRIMER_RIGHT_PENALTY=0.001908
PRIMER_LEFT_SEQUENCE=ACTCCCACCAACTACTCTTAGGG
PRIMER_RIGHT_SEQUENCE=AGTTTTAGCACTGCGTGTTCTTC
PRIMER_LEFT=1990,23
PRIMER_RIGHT=2485,23
PRIMER_LEFT_TM=59.937
PRIMER_RIGHT_TM=60.002
PRIMER_LEFT_GC_PERCENT=52.174
PRIMER_RIGHT_GC_PERCENT=43.478
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_MISPRIMING_SCORE=11.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_MISPRIMING_SCORE=20.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_END_STABILITY=8.7000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=496
PRIMER_PAIR_PENALTY_1=0.1423
PRIMER_LEFT_1_PENALTY=0.140398
PRIMER_RIGHT_1_PENALTY=0.001908
PRIMER_LEFT_1_SEQUENCE=AGGACCCACTATACCTTGCATTT
PRIMER_RIGHT_1_SEQUENCE=AGTTTTAGCACTGCGTGTTCTTC
PRIMER_LEFT_1=2067,23
PRIMER_RIGHT_1=2485,23
PRIMER_LEFT_1_TM=60.140
PRIMER_RIGHT_1_TM=60.002
PRIMER_LEFT_1_GC_PERCENT=43.478
PRIMER_RIGHT_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_MISPRIMING_SCORE=11.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_1_MISPRIMING_SCORE=21.00, reverse MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=419
PRIMER_PAIR_PENALTY_2=0.1698
PRIMER_LEFT_2_PENALTY=0.167921
PRIMER_RIGHT_2_PENALTY=0.001908
PRIMER_LEFT_2_SEQUENCE=ATGAACATAAGTGCTTAGCTGCC
PRIMER_RIGHT_2_SEQUENCE=AGTTTTAGCACTGCGTGTTCTTC
PRIMER_LEFT_2=1580,23
PRIMER_RIGHT_2=2485,23
PRIMER_LEFT_2_TM=59.832
PRIMER_RIGHT_2_TM=60.002
PRIMER_LEFT_2_GC_PERCENT=43.478
PRIMER_RIGHT_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, Tigger1 Autonomous DNA transposon
PRIMER_RIGHT_2_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_2_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=906
PRIMER_PAIR_PENALTY_3=0.1771
PRIMER_LEFT_3_PENALTY=0.175168
PRIMER_RIGHT_3_PENALTY=0.001908
PRIMER_LEFT_3_SEQUENCE=GTGATTCCAAGATCATCCTTCAC
PRIMER_RIGHT_3_SEQUENCE=AGTTTTAGCACTGCGTGTTCTTC
PRIMER_LEFT_3=1673,23
PRIMER_RIGHT_3=2485,23
PRIMER_LEFT_3_TM=59.825
PRIMER_RIGHT_3_TM=60.002
PRIMER_LEFT_3_GC_PERCENT=43.478
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, reverse MER4A a consensus
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_3_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=813
PRIMER_PAIR_PENALTY_4=0.2038
PRIMER_LEFT_4_PENALTY=0.063381
PRIMER_RIGHT_4_PENALTY=0.140398
PRIMER_LEFT_4_SEQUENCE=ACTCCCACCAACTACTCTTAGGG
PRIMER_RIGHT_4_SEQUENCE=AAATGCAAGGTATAGTGGGTCCT
PRIMER_LEFT_4=1990,23
PRIMER_RIGHT_4=2089,23
PRIMER_LEFT_4_TM=59.937
PRIMER_RIGHT_4_TM=60.140
PRIMER_LEFT_4_GC_PERCENT=52.174
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_MISPRIMING_SCORE=11.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_4_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_4_MISPRIMING_SCORE=20.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_4_END_STABILITY=8.7000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=100
PRIMER_PAIR_PENALTY_5=0.2093
PRIMER_LEFT_5_PENALTY=0.207373
PRIMER_RIGHT_5_PENALTY=0.001908
PRIMER_LEFT_5_SEQUENCE=GAACCAGGACTCCTCACTGTCTA
PRIMER_RIGHT_5_SEQUENCE=AGTTTTAGCACTGCGTGTTCTTC
PRIMER_LEFT_5=2170,23
PRIMER_RIGHT_5=2485,23
PRIMER_LEFT_5_TM=59.793
PRIMER_RIGHT_5_TM=60.002
PRIMER_LEFT_5_GC_PERCENT=52.174
PRIMER_RIGHT_5_GC_PERCENT=43.478
PRIMER_LEFT_5_SELF_ANY=7.00
PRIMER_RIGHT_5_SELF_ANY=5.00
PRIMER_LEFT_5_SELF_END=2.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_MISPRIMING_SCORE=11.00, L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_RIGHT_5_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_5_MISPRIMING_SCORE=21.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_5_END_STABILITY=5.4000
PRIMER_RIGHT_5_END_STABILITY=6.7000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_5=316
PRIMER_PAIR_PENALTY_6=0.2093
PRIMER_LEFT_6_PENALTY=0.207373
PRIMER_RIGHT_6_PENALTY=0.001908
PRIMER_LEFT_6_SEQUENCE=TCAGGTCTACCTCAGGACTGAAC
PRIMER_RIGHT_6_SEQUENCE=AGTTTTAGCACTGCGTGTTCTTC
PRIMER_LEFT_6=2250,23
PRIMER_RIGHT_6=2485,23
PRIMER_LEFT_6_TM=59.793
PRIMER_RIGHT_6_TM=60.002
PRIMER_LEFT_6_GC_PERCENT=52.174
PRIMER_RIGHT_6_GC_PERCENT=43.478
PRIMER_LEFT_6_SELF_ANY=6.00
PRIMER_RIGHT_6_SELF_ANY=5.00
PRIMER_LEFT_6_SELF_END=2.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_MISPRIMING_SCORE=12.00, MLT2C2 Interspersed repeat MLT2C2 - a consensus
PRIMER_RIGHT_6_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_6_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_6_END_STABILITY=6.7000
PRIMER_RIGHT_6_END_STABILITY=6.7000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_6=236
PRIMER_PAIR_PENALTY_7=0.2116
PRIMER_LEFT_7_PENALTY=0.053769
PRIMER_RIGHT_7_PENALTY=0.157853
PRIMER_LEFT_7_SEQUENCE=GCAAGGAGAAATGTGTCCACTAC
PRIMER_RIGHT_7_SEQUENCE=CACAATACCCAGAATGTCCACTT
PRIMER_LEFT_7=729,23
PRIMER_RIGHT_7=1099,23
PRIMER_LEFT_7_TM=60.054
PRIMER_RIGHT_7_TM=60.158
PRIMER_LEFT_7_GC_PERCENT=47.826
PRIMER_RIGHT_7_GC_PERCENT=43.478
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=2.00
PRIMER_RIGHT_7_SELF_END=2.00
PRIMER_LEFT_7_MISPRIMING_SCORE=12.00, reverse MER42a MER42 repetitive sequence: subfamily MER42a - a consensus
PRIMER_RIGHT_7_MISPRIMING_SCORE=12.00, reverse L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_PAIR_7_MISPRIMING_SCORE=22.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_7_END_STABILITY=5.1000
PRIMER_RIGHT_7_END_STABILITY=6.7000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_7=371
PRIMER_PAIR_PENALTY_8=0.2212
PRIMER_LEFT_8_PENALTY=0.157853
PRIMER_RIGHT_8_PENALTY=0.063381
PRIMER_LEFT_8_SEQUENCE=AAGTGGACATTCTGGGTATTGTG
PRIMER_RIGHT_8_SEQUENCE=CCCTAAGAGTAGTTGGTGGGAGT
PRIMER_LEFT_8=1077,23
PRIMER_RIGHT_8=2012,23
PRIMER_LEFT_8_TM=60.158
PRIMER_RIGHT_8_TM=59.937
PRIMER_LEFT_8_GC_PERCENT=43.478
PRIMER_RIGHT_8_GC_PERCENT=52.174
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_MISPRIMING_SCORE=12.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, reverse L1PA7 3'-end of L1 repeat (subfamily L1PA7) - a consensus sequence
PRIMER_PAIR_8_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_8_END_STABILITY=7.0000
PRIMER_RIGHT_8_END_STABILITY=7.6000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_8=936
PRIMER_PAIR_PENALTY_9=0.2265
PRIMER_LEFT_9_PENALTY=0.157853
PRIMER_RIGHT_9_PENALTY=0.068602
PRIMER_LEFT_9_SEQUENCE=AAGTGGACATTCTGGGTATTGTG
PRIMER_RIGHT_9_SEQUENCE=GGAAGCAAAACAGAGACTGCTTA
PRIMER_LEFT_9=1077,23
PRIMER_RIGHT_9=2039,23
PRIMER_LEFT_9_TM=60.158
PRIMER_RIGHT_9_TM=60.069
PRIMER_LEFT_9_GC_PERCENT=43.478
PRIMER_RIGHT_9_GC_PERCENT=43.478
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=5.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=3.00
PRIMER_LEFT_9_MISPRIMING_SCORE=12.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_9_MISPRIMING_SCORE=24.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_9_END_STABILITY=7.0000
PRIMER_RIGHT_9_END_STABILITY=7.5000
PRIMER_PAIR_9_COMPL_ANY=6.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_9=963
PRIMER_PAIR_PENALTY_10=0.2289
PRIMER_LEFT_10_PENALTY=0.053769
PRIMER_RIGHT_10_PENALTY=0.175168
PRIMER_LEFT_10_SEQUENCE=GCAAGGAGAAATGTGTCCACTAC
PRIMER_RIGHT_10_SEQUENCE=GTGAAGGATGATCTTGGAATCAC
PRIMER_LEFT_10=729,23
PRIMER_RIGHT_10=1695,23
PRIMER_LEFT_10_TM=60.054
PRIMER_RIGHT_10_TM=59.825
PRIMER_LEFT_10_GC_PERCENT=47.826
PRIMER_RIGHT_10_GC_PERCENT=43.478
PRIMER_LEFT_10_SELF_ANY=6.00
PRIMER_RIGHT_10_SELF_ANY=5.00
PRIMER_LEFT_10_SELF_END=2.00
PRIMER_RIGHT_10_SELF_END=2.00
PRIMER_LEFT_10_MISPRIMING_SCORE=12.00, reverse MER42a MER42 repetitive sequence: subfamily MER42a - a consensus
PRIMER_RIGHT_10_MISPRIMING_SCORE=11.00, MLT1f (MLT1f subfamily) - consensus sequence
PRIMER_PAIR_10_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_10_END_STABILITY=5.1000
PRIMER_RIGHT_10_END_STABILITY=6.3000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_10=967
PRIMER_PAIR_PENALTY_11=0.2313
PRIMER_LEFT_11_PENALTY=0.167921
PRIMER_RIGHT_11_PENALTY=0.063381
PRIMER_LEFT_11_SEQUENCE=ATGAACATAAGTGCTTAGCTGCC
PRIMER_RIGHT_11_SEQUENCE=CCCTAAGAGTAGTTGGTGGGAGT
PRIMER_LEFT_11=1580,23
PRIMER_RIGHT_11=2012,23
PRIMER_LEFT_11_TM=59.832
PRIMER_RIGHT_11_TM=59.937
PRIMER_LEFT_11_GC_PERCENT=43.478
PRIMER_RIGHT_11_GC_PERCENT=52.174
PRIMER_LEFT_11_SELF_ANY=6.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=2.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_MISPRIMING_SCORE=12.00, Tigger1 Autonomous DNA transposon
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, reverse L1PA7 3'-end of L1 repeat (subfamily L1PA7) - a consensus sequence
PRIMER_PAIR_11_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_11_END_STABILITY=9.7000
PRIMER_RIGHT_11_END_STABILITY=7.6000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_11=433
PRIMER_PAIR_PENALTY_12=0.2385
PRIMER_LEFT_12_PENALTY=0.175168
PRIMER_RIGHT_12_PENALTY=0.063381
PRIMER_LEFT_12_SEQUENCE=GTGATTCCAAGATCATCCTTCAC
PRIMER_RIGHT_12_SEQUENCE=CCCTAAGAGTAGTTGGTGGGAGT
PRIMER_LEFT_12=1673,23
PRIMER_RIGHT_12=2012,23
PRIMER_LEFT_12_TM=59.825
PRIMER_RIGHT_12_TM=59.937
PRIMER_LEFT_12_GC_PERCENT=43.478
PRIMER_RIGHT_12_GC_PERCENT=52.174
PRIMER_LEFT_12_SELF_ANY=5.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=2.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_MISPRIMING_SCORE=12.00, reverse MER4A a consensus
PRIMER_RIGHT_12_MISPRIMING_SCORE=12.00, reverse L1PA7 3'-end of L1 repeat (subfamily L1PA7) - a consensus sequence
PRIMER_PAIR_12_MISPRIMING_SCORE=22.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_12_END_STABILITY=6.7000
PRIMER_RIGHT_12_END_STABILITY=7.6000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_12=340
PRIMER_PAIR_PENALTY_13=0.2438
PRIMER_LEFT_13_PENALTY=0.175168
PRIMER_RIGHT_13_PENALTY=0.068602
PRIMER_LEFT_13_SEQUENCE=GTGATTCCAAGATCATCCTTCAC
PRIMER_RIGHT_13_SEQUENCE=GGAAGCAAAACAGAGACTGCTTA
PRIMER_LEFT_13=1673,23
PRIMER_RIGHT_13=2039,23
PRIMER_LEFT_13_TM=59.825
PRIMER_RIGHT_13_TM=60.069
PRIMER_LEFT_13_GC_PERCENT=43.478
PRIMER_RIGHT_13_GC_PERCENT=43.478
PRIMER_LEFT_13_SELF_ANY=5.00
PRIMER_RIGHT_13_SELF_ANY=5.00
PRIMER_LEFT_13_SELF_END=2.00
PRIMER_RIGHT_13_SELF_END=3.00
PRIMER_LEFT_13_MISPRIMING_SCORE=12.00, reverse MER4A a consensus
PRIMER_RIGHT_13_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_13_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_13_END_STABILITY=6.7000
PRIMER_RIGHT_13_END_STABILITY=7.5000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_13=367
PRIMER_PAIR_PENALTY_14=0.2504
PRIMER_LEFT_14_PENALTY=0.038458
PRIMER_RIGHT_14_PENALTY=0.211969
PRIMER_LEFT_14_SEQUENCE=CAGGACTTCTCTCCTGAAGAACA
PRIMER_RIGHT_14_SEQUENCE=TTGGTTACTGTTCTGGAGCTTGT
PRIMER_LEFT_14=2448,23
PRIMER_RIGHT_14=2772,23
PRIMER_LEFT_14_TM=60.038
PRIMER_RIGHT_14_TM=60.212
PRIMER_LEFT_14_GC_PERCENT=47.826
PRIMER_RIGHT_14_GC_PERCENT=43.478
PRIMER_LEFT_14_SELF_ANY=6.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=3.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_14_MISPRIMING_SCORE=12.00, Tigger2 Autonomous DNA transposon
PRIMER_PAIR_14_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_14_END_STABILITY=6.7000
PRIMER_RIGHT_14_END_STABILITY=6.7000
PRIMER_PAIR_14_COMPL_ANY=6.00
PRIMER_PAIR_14_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_14=325
PRIMER_PAIR_PENALTY_15=0.2646
PRIMER_LEFT_15_PENALTY=0.262717
PRIMER_RIGHT_15_PENALTY=0.001908
PRIMER_LEFT_15_SEQUENCE=TATGCAGGACCCACTATACCTTG
PRIMER_RIGHT_15_SEQUENCE=AGTTTTAGCACTGCGTGTTCTTC
PRIMER_LEFT_15=2062,23
PRIMER_RIGHT_15=2485,23
PRIMER_LEFT_15_TM=60.263
PRIMER_RIGHT_15_TM=60.002
PRIMER_LEFT_15_GC_PERCENT=47.826
PRIMER_RIGHT_15_GC_PERCENT=43.478
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=5.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_15_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_15_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_15_END_STABILITY=8.5000
PRIMER_RIGHT_15_END_STABILITY=6.7000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_15=424
PRIMER_PAIR_PENALTY_16=0.2698
PRIMER_LEFT_16_PENALTY=0.215994
PRIMER_RIGHT_16_PENALTY=0.053769
PRIMER_LEFT_16_SEQUENCE=AGGAGGACGGAGATTACATCAAT
PRIMER_RIGHT_16_SEQUENCE=GTAGTGGACACATTTCTCCTTGC
PRIMER_LEFT_16=561,23
PRIMER_RIGHT_16=751,23
PRIMER_LEFT_16_TM=60.216
PRIMER_RIGHT_16_TM=60.054
PRIMER_LEFT_16_GC_PERCENT=43.478
PRIMER_RIGHT_16_GC_PERCENT=47.826
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=6.00
PRIMER_LEFT_16_SELF_END=2.00
PRIMER_RIGHT_16_SELF_END=2.00
PRIMER_LEFT_16_MISPRIMING_SCORE=12.00, MER21B a consensus
PRIMER_RIGHT_16_MISPRIMING_SCORE=12.00, reverse MER42a MER42 repetitive sequence: subfamily MER42a - a consensus
PRIMER_PAIR_16_MISPRIMING_SCORE=22.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_16_END_STABILITY=6.9000
PRIMER_RIGHT_16_END_STABILITY=8.5000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_16=191
PRIMER_PAIR_PENALTY_17=0.2708
PRIMER_LEFT_17_PENALTY=0.063381
PRIMER_RIGHT_17_PENALTY=0.207373
PRIMER_LEFT_17_SEQUENCE=ACTCCCACCAACTACTCTTAGGG
PRIMER_RIGHT_17_SEQUENCE=TAGACAGTGAGGAGTCCTGGTTC
PRIMER_LEFT_17=1990,23
PRIMER_RIGHT_17=2192,23
PRIMER_LEFT_17_TM=59.937
PRIMER_RIGHT_17_TM=59.793
PRIMER_LEFT_17_GC_PERCENT=52.174
PRIMER_RIGHT_17_GC_PERCENT=52.174
PRIMER_LEFT_17_SELF_ANY=3.00
PRIMER_RIGHT_17_SELF_ANY=7.00
PRIMER_LEFT_17_SELF_END=3.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_MISPRIMING_SCORE=11.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_17_MISPRIMING_SCORE=12.00, MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_PAIR_17_MISPRIMING_SCORE=20.00, L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_LEFT_17_END_STABILITY=8.7000
PRIMER_RIGHT_17_END_STABILITY=7.9000
PRIMER_PAIR_17_COMPL_ANY=6.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_17=203
PRIMER_PAIR_PENALTY_18=0.2753
PRIMER_LEFT_18_PENALTY=0.063381
PRIMER_RIGHT_18_PENALTY=0.211969
PRIMER_LEFT_18_SEQUENCE=ACTCCCACCAACTACTCTTAGGG
PRIMER_RIGHT_18_SEQUENCE=TTGGTTACTGTTCTGGAGCTTGT
PRIMER_LEFT_18=1990,23
PRIMER_RIGHT_18=2772,23
PRIMER_LEFT_18_TM=59.937
PRIMER_RIGHT_18_TM=60.212
PRIMER_LEFT_18_GC_PERCENT=52.174
PRIMER_RIGHT_18_GC_PERCENT=43.478
PRIMER_LEFT_18_SELF_ANY=3.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=3.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_MISPRIMING_SCORE=11.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_18_MISPRIMING_SCORE=12.00, Tigger2 Autonomous DNA transposon
PRIMER_PAIR_18_MISPRIMING_SCORE=22.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_18_END_STABILITY=8.7000
PRIMER_RIGHT_18_END_STABILITY=6.7000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_18=783
PRIMER_PAIR_PENALTY_19=0.2843
PRIMER_LEFT_19_PENALTY=0.282351
PRIMER_RIGHT_19_PENALTY=0.001908
PRIMER_LEFT_19_SEQUENCE=CATCCTATCAGTGACTCTGCTCC
PRIMER_RIGHT_19_SEQUENCE=AGTTTTAGCACTGCGTGTTCTTC
PRIMER_LEFT_19=1767,23
PRIMER_RIGHT_19=2485,23
PRIMER_LEFT_19_TM=60.282
PRIMER_RIGHT_19_TM=60.002
PRIMER_LEFT_19_GC_PERCENT=52.174
PRIMER_RIGHT_19_GC_PERCENT=43.478
PRIMER_LEFT_19_SELF_ANY=5.00
PRIMER_RIGHT_19_SELF_ANY=5.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_MISPRIMING_SCORE=11.00, THE1b consensus sequence
PRIMER_RIGHT_19_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_19_MISPRIMING_SCORE=22.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_19_END_STABILITY=9.4000
PRIMER_RIGHT_19_END_STABILITY=6.7000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_19=719
=