PRIMER_SEQUENCE_ID=NM_002833
SEQUENCE=GGCTGAGGCAGCCGCGCAGGTCGCAGGGCCAGCGTCCGCGAGGACGGCCCGGCTGGGCGGCCCCGAGCTCTGTGGCGCTGTGGAGGAGCGGGAGCGCGGCCGAGAAAGCGGGGCGCCGAGGGGGTCGGCGGCCTTCGGGAAATTTCCGCCGACCCTTCGCTCCCGGCTCTAAAAGTTCCTGATTTCCTATTTCCTTTTAAATCCCGAGTGGCTGTTAGCTCTTCGCCTGCACTTTTTCTTCCCCAGGAGATAAGGGGGAGTGTGAGGAACGGAGCGAATAATATAAAAAAGGATTTCCTCCCGGAAGAGAGCGGCAGTTCGGAGAGATTTTTCTTAAGGAAGCAGAAGCGGCGTTTGCGGCCGCTGCAGGCGCCGGGCCCTGCCGGCCACACTATGCGCGAGCCGGCCCCGGGCTGCTGAGGCGCGGGGACGCGGAAGCGGAGGCCGAGCGCGCCGGGCTCCCGCGCTCGCGAGCGAGTTTTGTCCGCCCGGCGGCGGTGGCGGGGGGATGGAGCCCGCGACCGCGCCCCGGCCCGACATGGCGCCGGAGCTGACCCCGGAGGAGGAGCAGGCTACCAAGCAGTTTCTCGAAGAGATTAACAAGTGGACAGTTCAGTACAATGTTTCCCCGCTGTCTTGGAATGTGGCTGTCAAGTTCCTCATGGCAAGGAAGTTTGATGTGCTCCGTGCCATAGAATTGTTCCACTCCTACAGAGAAACTCGAAGGAAGGAAGGCATTGTAAAGCTGAAACCTCATGAGGAACCTCTTCGTTCTGAGATCCTCAGTGGAAAATTCACCATCTTAAATGTTCGGGACCCAACAGGAGCCTCCATTGCCCTCTTTACTGCCAGGTTGCATCATCCCCACAAGTCAGTCCAACATGTGGTACTTCAGGCTCTGTTTTACTTGCTAGACAGAGCTGTGGATAGCTTTGAAACTCAGAGGAATGGACTGGTGTTTATCTATGACATGTGTGGTTCTAATTATGCCAACTTTGAGCTGGATCTTGGCAAGAAAGTCCTAAACCTGCTGAAGGGAGCATTTCCAGCTCGTTTGAAGAAGGTGCTGATTGTGGGGGCACCCATATGGTTCCGAGTGCCCTATTCCATCATCAGTCTCCTCCTGAAGGACAAAGTCCGGGAGAGGATTCAAATATTAAAGACATCTGAGGTCACGCAGCATCTGCCCAGGGAGTGTCTTCCAGAAAACCTGGGTGGGTACGTCAAAATTGATCTCGCCACTTGGAATTTCCAGTTCCTACCCCAGGTGAACGGCCACCCAGATCCCTTCGATGAGATCATCCTGTTCTCCCTCCCTCCTGCCTTAGACTGGGACTCAGTACATGTTCCAGGTCCCCATGCTATGACCATCCAAGAGTTGGTGGACTATGTTAATGCCAGGCAAAAGCAAGGAATCTATGAGGAATATGAAGACATTCGTCGTGAGAACCCTGTTGGCACTTTCCACTGTTCCATGTCTCCAGGAAACCTAGAGAAAAACCGTTATGGGGATGTACCCTGCCTGGACCAAACTAGAGTGAAGCTAACAAAGCGAAGTGGCCATACTCAGACAGATTACATCAATGCCAGTTTCATGGATGGCTACAAGCAGAAGAATGCTTACATTGGCACACAAGGTCCTTTGGAGAATACCTATCGTGATTTCTGGCTCATGGTATGGGAGCAAAAAGTCTTGGTGATTGTCATGACCACCCGCTTTGAGGAAGGCGGCAGGAGAAAGTGTGGCCAGTACTGGCCTTTAGAAAAAGACTCTCGGATCCGATTTGGCTTCCTCACAGTGACCAATCTAGGCGTGGAGAACATGAATCATTATAAGAAAACAACGCTAGAAATTCACAACACAGAGGAACGGCAGAAACGCCAGGTGACCCACTTCCAGTTCTTGAGCTGGCCAGACTATGGTGTCCCTTCCTCAGCAGCTTCCCTCATTGACTTCTTGAGAGTGGTCAGAAACCAGCAGAGTCTGGCTGTGAGCAACATGGGAGCACGCTCCAAAGGGCAGTGCCCTGAGCCACCCATTGTGGTCCATTGCAGTGCAGGCATTGGCAGGACAGGTACCTTCTGCTCACTGGACATCTGCCTGGCACAGCTGGAGGAGCTTGGCACCCTTAATGTGTTCCAGACGGTGTCACGCATGAGGACCCAGAGGGCCTTCAGCATCCAGACCCCTGAGCAGTACTATTTTTGCTACAAGGCCATCCTGGAGTTCGCAGAGAAGGAGGGCATGGTATCCTCTGGCCAAAACCTGCTGGCCGTGGAGAGTCAGTAACTCTCCTACGAACCTCCTACCTGTTGGCCAGCCTTCCTTAAACTACCCTGGACACCGCTGAGCCTATAGGTTGCCATCAGTTACGCTGAAGCCATGGATCAACTTCTTTCTTGTGTCTCCCAGCACACGTGTGCACGCAAGGCAGCCTTTCCCTTTGGCTAGATAAATGTGGTGAAATTGCCACTAGAAAGGGCCAGTAGCAATGTGTTCTTAATTCCTAGCATCTGCTATCAAACTGTGCCTTATTAAAACCAAATTGTGGCTATTGATTTTTGCTAGGGTCCCCGAGGTAAGTGGGGAAGGAACCTCCCTCCTCCTTTCTCCCGCCATTCTTTTTCTGAGGTCTCCTGTCTCCCACTCCTTCCCTTTCCTAGGATTTTATGGGGAGGTTTGATGAGCATTTGCCTTTCTCCCCAGAGAGCTTGACCAAGTTACATATTCTAGAGATCGTCCTGAGTGCCAAGCACGTTTATAAGTAGGGCGTGTATTCCAGTCCTTTCTGTCATTCTGTGTGTGTGTGTGTGTGCACTTATGTGTATGGGTCCATATGTACGTGTGTATATAATATATATTGTATGTGATAATATGATCGTATTCTATAAATACATATACACACGATACTCCTTTCCAGAAGTTCCTGGGGCTCCGTGTTGCAGTTCAGGACTCCTTGCTTCTTGGACCCTACTATTTATCCTGGACTAGCTTGGGTTGGTGATCATGTCTCCTCTTGTCAGGCTACAGAGTGGTGGAAGAGGACACACACACATCTCCTCCATGTTCCTGCCACAGGGCCCCTTCCTTAAGTAATGACTTATCTCCTTCCAGTTGCCACCTGTTGGAGCCAAGAATGTATTAGTTTGTGGTGACACTGGGTTAGCTCAGCATGGATTTCCCTTATCCGATGATTTTTCTACATTTACTTGGCCAATTTGGGGAACAGACCTCCACTGTGATTCCATACTCTCTCTTTGCTTTCACTATCCCAGGGATGGGAGGCGGAGGGTAGAGATGAACTCACTGAGCAGCCAGAGCCAAATGCAATCGGTACGAATCTTAGAAGGAAGGAGGGGGAGCCCAGGTATGAGAAAGAAAAAACCACTAAGAAAATACCTCCCTGGGAGGATGAGCTGGGGCCCTTTTTCTTTTGCTGGATGGTTCCTTTATGCAGCTTGGCCCTGTCTACCGAGATGCCCATCTCTTCCTGCCTGCTAGCCTGCTAGACCCTCAAACTGGGTGGGTTCTGTGTCAATAAAAAGCTTCACCCCCTGGCTGAGTGAGGTGGTCCCCTGCAATCACTGTTTGTCCCCTACCCACCCAACCTGTCCCTGCCTGCTCCCAGCCCACTCATCCTTATGTGCTAGGGATAAATCAAGAGTCCTCAGCACTCCACATTCCCAAAAAATCCCAGGAACTCCTAAACCTTCCCCTGTGACAGAAGATGAGGTTGGCAGCTGATCAGACCTCAATAATTTTATACTGTAATAAGCTCTTTGAATGTGTATATTATTATTTTTTACAATCATTTCATTTTGTATTTAATATCAATGGACTGAGTCTGATTCAGAAAATAATTGTAATGTTCATATTCAATATCTTACAGTGATACAGTTTTGTATTTACATATAAATATACCGACATGATCAAAACCAAAAAAAAAAAAAAAAAA
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 45715, GC content failed 2939, low tm 17409, high tm 15446, high end compl 23, high repeat similarity 31, long poly-x seq 75, ok 9792
PRIMER_RIGHT_EXPLAIN=considered 45645, GC content failed 2816, low tm 17973, high tm 14942, high end compl 9, high repeat similarity 15, long poly-x seq 87, ok 9803
PRIMER_PAIR_EXPLAIN=considered 510, unacceptable product size 458, high any compl 2, high end compl 7, ok 43
PRIMER_PAIR_PENALTY=0.0339
PRIMER_LEFT_PENALTY=0.030877
PRIMER_RIGHT_PENALTY=0.003045
PRIMER_LEFT_SEQUENCE=CCTTGCTTCTTGGACCCTACTAT
PRIMER_RIGHT_SEQUENCE=TCTAAGATTCGTACCGATTGCAT
PRIMER_LEFT=2955,23
PRIMER_RIGHT=3347,23
PRIMER_LEFT_TM=60.031
PRIMER_RIGHT_TM=60.003
PRIMER_LEFT_GC_PERCENT=47.826
PRIMER_RIGHT_GC_PERCENT=39.130
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_MISPRIMING_SCORE=11.00, Tigger2 Autonomous DNA transposon
PRIMER_RIGHT_MISPRIMING_SCORE=10.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_END_STABILITY=5.3000
PRIMER_RIGHT_END_STABILITY=8.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=393
PRIMER_PAIR_PENALTY_1=0.0500
PRIMER_LEFT_1_PENALTY=0.046971
PRIMER_RIGHT_1_PENALTY=0.003045
PRIMER_LEFT_1_SEQUENCE=CGTGTATTCCAGTCCTTTCTGTC
PRIMER_RIGHT_1_SEQUENCE=TCTAAGATTCGTACCGATTGCAT
PRIMER_LEFT_1=2771,23
PRIMER_RIGHT_1=3347,23
PRIMER_LEFT_1_TM=60.047
PRIMER_RIGHT_1_TM=60.003
PRIMER_LEFT_1_GC_PERCENT=47.826
PRIMER_RIGHT_1_GC_PERCENT=39.130
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_1_MISPRIMING_SCORE=10.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_1_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=577
PRIMER_PAIR_PENALTY_2=0.0794
PRIMER_LEFT_2_PENALTY=0.076327
PRIMER_RIGHT_2_PENALTY=0.003045
PRIMER_LEFT_2_SEQUENCE=CTAGGATTTTATGGGGAGGTTTG
PRIMER_RIGHT_2_SEQUENCE=TCTAAGATTCGTACCGATTGCAT
PRIMER_LEFT_2=2660,23
PRIMER_RIGHT_2=3347,23
PRIMER_LEFT_2_TM=60.076
PRIMER_RIGHT_2_TM=60.003
PRIMER_LEFT_2_GC_PERCENT=43.478
PRIMER_RIGHT_2_GC_PERCENT=39.130
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_2_MISPRIMING_SCORE=10.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_2_MISPRIMING_SCORE=20.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=688
PRIMER_PAIR_PENALTY_3=0.0870
PRIMER_LEFT_3_PENALTY=0.056142
PRIMER_RIGHT_3_PENALTY=0.030877
PRIMER_LEFT_3_SEQUENCE=TATAGGTTGCCATCAGTTACGCT
PRIMER_RIGHT_3_SEQUENCE=ATAGTAGGGTCCAAGAAGCAAGG
PRIMER_LEFT_3=2354,23
PRIMER_RIGHT_3=2977,23
PRIMER_LEFT_3_TM=60.056
PRIMER_RIGHT_3_TM=60.031
PRIMER_LEFT_3_GC_PERCENT=43.478
PRIMER_RIGHT_3_GC_PERCENT=47.826
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_3_MISPRIMING_SCORE=24.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_3_END_STABILITY=9.6000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=624
PRIMER_PAIR_PENALTY_4=0.0870
PRIMER_LEFT_4_PENALTY=0.056142
PRIMER_RIGHT_4_PENALTY=0.030877
PRIMER_LEFT_4_SEQUENCE=CTATAGGTTGCCATCAGTTACGC
PRIMER_RIGHT_4_SEQUENCE=ATAGTAGGGTCCAAGAAGCAAGG
PRIMER_LEFT_4=2353,23
PRIMER_RIGHT_4=2977,23
PRIMER_LEFT_4_TM=60.056
PRIMER_RIGHT_4_TM=60.031
PRIMER_LEFT_4_GC_PERCENT=47.826
PRIMER_RIGHT_4_GC_PERCENT=47.826
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_4_MISPRIMING_SCORE=24.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_4_END_STABILITY=8.9000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=625
PRIMER_PAIR_PENALTY_5=0.0914
PRIMER_LEFT_5_PENALTY=0.088390
PRIMER_RIGHT_5_PENALTY=0.003045
PRIMER_LEFT_5_SEQUENCE=AAGAGGACACACACACATCTCCT
PRIMER_RIGHT_5_SEQUENCE=TCTAAGATTCGTACCGATTGCAT
PRIMER_LEFT_5=3039,23
PRIMER_RIGHT_5=3347,23
PRIMER_LEFT_5_TM=60.088
PRIMER_RIGHT_5_TM=60.003
PRIMER_LEFT_5_GC_PERCENT=47.826
PRIMER_RIGHT_5_GC_PERCENT=39.130
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=5.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=2.00
PRIMER_LEFT_5_MISPRIMING_SCORE=12.00, reverse MER6 Nonautonomous DNA transposon
PRIMER_RIGHT_5_MISPRIMING_SCORE=10.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_5_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_5_END_STABILITY=7.9000
PRIMER_RIGHT_5_END_STABILITY=8.4000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_5=309
PRIMER_PAIR_PENALTY_6=0.0933
PRIMER_LEFT_6_PENALTY=0.062384
PRIMER_RIGHT_6_PENALTY=0.030877
PRIMER_LEFT_6_SEQUENCE=CTCCTACGAACCTCCTACCTGTT
PRIMER_RIGHT_6_SEQUENCE=ATAGTAGGGTCCAAGAAGCAAGG
PRIMER_LEFT_6=2292,23
PRIMER_RIGHT_6=2977,23
PRIMER_LEFT_6_TM=60.062
PRIMER_RIGHT_6_TM=60.031
PRIMER_LEFT_6_GC_PERCENT=52.174
PRIMER_RIGHT_6_GC_PERCENT=47.826
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_MISPRIMING_SCORE=12.00, reverse L1MA2 3'-end of L1 repeat (subfamily L1MA2) - a consensus sequence
PRIMER_RIGHT_6_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_6_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_6_END_STABILITY=6.7000
PRIMER_RIGHT_6_END_STABILITY=8.5000
PRIMER_PAIR_6_COMPL_ANY=7.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_6=686
PRIMER_PAIR_PENALTY_7=0.0974
PRIMER_LEFT_7_PENALTY=0.094389
PRIMER_RIGHT_7_PENALTY=0.003045
PRIMER_LEFT_7_SEQUENCE=CCTTTGGCTAGATAAATGTGGTG
PRIMER_RIGHT_7_SEQUENCE=TCTAAGATTCGTACCGATTGCAT
PRIMER_LEFT_7=2441,23
PRIMER_RIGHT_7=3347,23
PRIMER_LEFT_7_TM=59.906
PRIMER_RIGHT_7_TM=60.003
PRIMER_LEFT_7_GC_PERCENT=43.478
PRIMER_RIGHT_7_GC_PERCENT=39.130
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=5.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=2.00
PRIMER_LEFT_7_MISPRIMING_SCORE=12.00, reverse L1PA7 3'-end of L1 repeat (subfamily L1PA7) - a consensus sequence
PRIMER_RIGHT_7_MISPRIMING_SCORE=10.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_7_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_7_END_STABILITY=8.2000
PRIMER_RIGHT_7_END_STABILITY=8.4000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_7=907
PRIMER_PAIR_PENALTY_8=0.1031
PRIMER_LEFT_8_PENALTY=0.056142
PRIMER_RIGHT_8_PENALTY=0.046971
PRIMER_LEFT_8_SEQUENCE=TATAGGTTGCCATCAGTTACGCT
PRIMER_RIGHT_8_SEQUENCE=GACAGAAAGGACTGGAATACACG
PRIMER_LEFT_8=2354,23
PRIMER_RIGHT_8=2793,23
PRIMER_LEFT_8_TM=60.056
PRIMER_RIGHT_8_TM=60.047
PRIMER_LEFT_8_GC_PERCENT=43.478
PRIMER_RIGHT_8_GC_PERCENT=47.826
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=2.00
PRIMER_LEFT_8_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_8_MISPRIMING_SCORE=24.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_8_END_STABILITY=9.6000
PRIMER_RIGHT_8_END_STABILITY=8.1000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_8=440
PRIMER_PAIR_PENALTY_9=0.1031
PRIMER_LEFT_9_PENALTY=0.056142
PRIMER_RIGHT_9_PENALTY=0.046971
PRIMER_LEFT_9_SEQUENCE=CTATAGGTTGCCATCAGTTACGC
PRIMER_RIGHT_9_SEQUENCE=GACAGAAAGGACTGGAATACACG
PRIMER_LEFT_9=2353,23
PRIMER_RIGHT_9=2793,23
PRIMER_LEFT_9_TM=60.056
PRIMER_RIGHT_9_TM=60.047
PRIMER_LEFT_9_GC_PERCENT=47.826
PRIMER_RIGHT_9_GC_PERCENT=47.826
PRIMER_LEFT_9_SELF_ANY=6.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=2.00
PRIMER_RIGHT_9_SELF_END=2.00
PRIMER_LEFT_9_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_9_MISPRIMING_SCORE=24.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_9_END_STABILITY=8.9000
PRIMER_RIGHT_9_END_STABILITY=8.1000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_9=441
PRIMER_PAIR_PENALTY_10=0.1072
PRIMER_LEFT_10_PENALTY=0.076327
PRIMER_RIGHT_10_PENALTY=0.030877
PRIMER_LEFT_10_SEQUENCE=CTAGGATTTTATGGGGAGGTTTG
PRIMER_RIGHT_10_SEQUENCE=ATAGTAGGGTCCAAGAAGCAAGG
PRIMER_LEFT_10=2660,23
PRIMER_RIGHT_10=2977,23
PRIMER_LEFT_10_TM=60.076
PRIMER_RIGHT_10_TM=60.031
PRIMER_LEFT_10_GC_PERCENT=43.478
PRIMER_RIGHT_10_GC_PERCENT=47.826
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_10_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_10_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_10_END_STABILITY=7.0000
PRIMER_RIGHT_10_END_STABILITY=8.5000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_10=318
PRIMER_PAIR_PENALTY_11=0.1094
PRIMER_LEFT_11_PENALTY=0.062384
PRIMER_RIGHT_11_PENALTY=0.046971
PRIMER_LEFT_11_SEQUENCE=CTCCTACGAACCTCCTACCTGTT
PRIMER_RIGHT_11_SEQUENCE=GACAGAAAGGACTGGAATACACG
PRIMER_LEFT_11=2292,23
PRIMER_RIGHT_11=2793,23
PRIMER_LEFT_11_TM=60.062
PRIMER_RIGHT_11_TM=60.047
PRIMER_LEFT_11_GC_PERCENT=52.174
PRIMER_RIGHT_11_GC_PERCENT=47.826
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=2.00
PRIMER_LEFT_11_MISPRIMING_SCORE=12.00, reverse L1MA2 3'-end of L1 repeat (subfamily L1MA2) - a consensus sequence
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_11_MISPRIMING_SCORE=23.00, reverse L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_LEFT_11_END_STABILITY=6.7000
PRIMER_RIGHT_11_END_STABILITY=8.1000
PRIMER_PAIR_11_COMPL_ANY=6.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_11=502
PRIMER_PAIR_PENALTY_12=0.1094
PRIMER_LEFT_12_PENALTY=0.065377
PRIMER_RIGHT_12_PENALTY=0.044016
PRIMER_LEFT_12_SEQUENCE=CAAGAAAGTCCTAAACCTGCTGA
PRIMER_RIGHT_12_SEQUENCE=ACGGTTTTTCTCTAGGTTTCCTG
PRIMER_LEFT_12=1011,23
PRIMER_RIGHT_12=1503,23
PRIMER_LEFT_12_TM=59.935
PRIMER_RIGHT_12_TM=60.044
PRIMER_LEFT_12_GC_PERCENT=43.478
PRIMER_RIGHT_12_GC_PERCENT=43.478
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_MISPRIMING_SCORE=11.00, MER34 a consensus
PRIMER_RIGHT_12_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_12_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_12_END_STABILITY=8.2000
PRIMER_RIGHT_12_END_STABILITY=8.2000
PRIMER_PAIR_12_COMPL_ANY=6.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_12=493
PRIMER_PAIR_PENALTY_13=0.1149
PRIMER_LEFT_13_PENALTY=0.065377
PRIMER_RIGHT_13_PENALTY=0.049500
PRIMER_LEFT_13_SEQUENCE=CAAGAAAGTCCTAAACCTGCTGA
PRIMER_RIGHT_13_SEQUENCE=TACTGAGTCCCAGTCTAAGGCAG
PRIMER_LEFT_13=1011,23
PRIMER_RIGHT_13=1341,23
PRIMER_LEFT_13_TM=59.935
PRIMER_RIGHT_13_TM=59.950
PRIMER_LEFT_13_GC_PERCENT=43.478
PRIMER_RIGHT_13_GC_PERCENT=52.174
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=7.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=3.00
PRIMER_LEFT_13_MISPRIMING_SCORE=11.00, MER34 a consensus
PRIMER_RIGHT_13_MISPRIMING_SCORE=12.00, reverse MER6 Nonautonomous DNA transposon
PRIMER_PAIR_13_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_13_END_STABILITY=8.2000
PRIMER_RIGHT_13_END_STABILITY=9.7000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_13=331
PRIMER_PAIR_PENALTY_14=0.1201
PRIMER_LEFT_14_PENALTY=0.070598
PRIMER_RIGHT_14_PENALTY=0.049500
PRIMER_LEFT_14_SEQUENCE=TTCCACTCCTACAGAGAAACTCG
PRIMER_RIGHT_14_SEQUENCE=TACTGAGTCCCAGTCTAAGGCAG
PRIMER_LEFT_14=700,23
PRIMER_RIGHT_14=1341,23
PRIMER_LEFT_14_TM=59.929
PRIMER_RIGHT_14_TM=59.950
PRIMER_LEFT_14_GC_PERCENT=47.826
PRIMER_RIGHT_14_GC_PERCENT=52.174
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=7.00
PRIMER_LEFT_14_SELF_END=2.00
PRIMER_RIGHT_14_SELF_END=3.00
PRIMER_LEFT_14_MISPRIMING_SCORE=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_14_MISPRIMING_SCORE=12.00, reverse MER6 Nonautonomous DNA transposon
PRIMER_PAIR_14_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_14_END_STABILITY=8.1000
PRIMER_RIGHT_14_END_STABILITY=9.7000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_14=642
PRIMER_PAIR_PENALTY_15=0.1201
PRIMER_LEFT_15_PENALTY=0.070598
PRIMER_RIGHT_15_PENALTY=0.049500
PRIMER_LEFT_15_SEQUENCE=CCACTCCTACAGAGAAACTCGAA
PRIMER_RIGHT_15_SEQUENCE=TACTGAGTCCCAGTCTAAGGCAG
PRIMER_LEFT_15=702,23
PRIMER_RIGHT_15=1341,23
PRIMER_LEFT_15_TM=59.929
PRIMER_RIGHT_15_TM=59.950
PRIMER_LEFT_15_GC_PERCENT=47.826
PRIMER_RIGHT_15_GC_PERCENT=52.174
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=7.00
PRIMER_LEFT_15_SELF_END=2.00
PRIMER_RIGHT_15_SELF_END=3.00
PRIMER_LEFT_15_MISPRIMING_SCORE=11.00, L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_RIGHT_15_MISPRIMING_SCORE=12.00, reverse MER6 Nonautonomous DNA transposon
PRIMER_PAIR_15_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_15_END_STABILITY=8.7000
PRIMER_RIGHT_15_END_STABILITY=9.7000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_15=640
PRIMER_PAIR_PENALTY_16=0.1233
PRIMER_LEFT_16_PENALTY=0.076327
PRIMER_RIGHT_16_PENALTY=0.046971
PRIMER_LEFT_16_SEQUENCE=CTAGGATTTTATGGGGAGGTTTG
PRIMER_RIGHT_16_SEQUENCE=GACAGAAAGGACTGGAATACACG
PRIMER_LEFT_16=2660,23
PRIMER_RIGHT_16=2793,23
PRIMER_LEFT_16_TM=60.076
PRIMER_RIGHT_16_TM=60.047
PRIMER_LEFT_16_GC_PERCENT=43.478
PRIMER_RIGHT_16_GC_PERCENT=47.826
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=3.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=2.00
PRIMER_LEFT_16_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_16_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_16_MISPRIMING_SCORE=22.00, MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_LEFT_16_END_STABILITY=7.0000
PRIMER_RIGHT_16_END_STABILITY=8.1000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_16=134
PRIMER_PAIR_PENALTY_17=0.1253
PRIMER_LEFT_17_PENALTY=0.094389
PRIMER_RIGHT_17_PENALTY=0.030877
PRIMER_LEFT_17_SEQUENCE=CCTTTGGCTAGATAAATGTGGTG
PRIMER_RIGHT_17_SEQUENCE=ATAGTAGGGTCCAAGAAGCAAGG
PRIMER_LEFT_17=2441,23
PRIMER_RIGHT_17=2977,23
PRIMER_LEFT_17_TM=59.906
PRIMER_RIGHT_17_TM=60.031
PRIMER_LEFT_17_GC_PERCENT=43.478
PRIMER_RIGHT_17_GC_PERCENT=47.826
PRIMER_LEFT_17_SELF_ANY=6.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_MISPRIMING_SCORE=12.00, reverse L1PA7 3'-end of L1 repeat (subfamily L1PA7) - a consensus sequence
PRIMER_RIGHT_17_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_17_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_17_END_STABILITY=8.2000
PRIMER_RIGHT_17_END_STABILITY=8.5000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_17=537
PRIMER_PAIR_PENALTY_18=0.1295
PRIMER_LEFT_18_PENALTY=0.085505
PRIMER_RIGHT_18_PENALTY=0.044016
PRIMER_LEFT_18_SEQUENCE=TCCAAGAGTTGGTGGACTATGTT
PRIMER_RIGHT_18_SEQUENCE=ACGGTTTTTCTCTAGGTTTCCTG
PRIMER_LEFT_18=1370,23
PRIMER_RIGHT_18=1503,23
PRIMER_LEFT_18_TM=59.914
PRIMER_RIGHT_18_TM=60.044
PRIMER_LEFT_18_GC_PERCENT=43.478
PRIMER_RIGHT_18_GC_PERCENT=43.478
PRIMER_LEFT_18_SELF_ANY=5.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_MISPRIMING_SCORE=12.00, MLT1c (MLT1c subfamily) - consensus sequence
PRIMER_RIGHT_18_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_18_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_18_END_STABILITY=6.6000
PRIMER_RIGHT_18_END_STABILITY=8.2000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_18=134
PRIMER_PAIR_PENALTY_19=0.1392
PRIMER_LEFT_19_PENALTY=0.136143
PRIMER_RIGHT_19_PENALTY=0.003045
PRIMER_LEFT_19_SEQUENCE=ACTCCTTCCCTTTCCTAGGATTT
PRIMER_RIGHT_19_SEQUENCE=TCTAAGATTCGTACCGATTGCAT
PRIMER_LEFT_19=2646,23
PRIMER_RIGHT_19=3347,23
PRIMER_LEFT_19_TM=59.864
PRIMER_RIGHT_19_TM=60.003
PRIMER_LEFT_19_GC_PERCENT=43.478
PRIMER_RIGHT_19_GC_PERCENT=39.130
PRIMER_LEFT_19_SELF_ANY=8.00
PRIMER_RIGHT_19_SELF_ANY=5.00
PRIMER_LEFT_19_SELF_END=2.00
PRIMER_RIGHT_19_SELF_END=2.00
PRIMER_LEFT_19_MISPRIMING_SCORE=12.00, MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_RIGHT_19_MISPRIMING_SCORE=10.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_19_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_19_END_STABILITY=6.9000
PRIMER_RIGHT_19_END_STABILITY=8.4000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_19=702
=