PRIMER_SEQUENCE_ID=NM_002834
SEQUENCE=CGGCCGCGGTTTCCAGGAGGAAGCAAGGATGCTTTGGACACTGTGCGTGGCGCCTCCGCGGAGCCCCCGCGCTGCCATTCCCGGCCGTCGCTCGGTCCTCCGCTGACGGGAAGCAGGAAGTGGCGGCGGGCGTCGCGAGCGGTGACATCACGGGGGCGACGGCGGCGAAGGGCGGGGGCGGAGGAGGAGCGAGCCGGGCCGGGGGGCAGCTGCACAGTCTCCGGGATCCCCAGGCCTGGAGGGGGGTCTGTGCGCGGCCGGCTGGCTCTGCCCCGCGTCCGGTCCCGAGCGGGCCTCCCTCGGGCCAGCCCGATGTGACCGAGCCCAGCGGAGCCTGAGCAAGGAGCGGGTCCGTCGCGGAGCCGGAGGGCGGGAGGAACATGACATCGCGGAGATGGTTTCACCCAAATATCACTGGTGTGGAGGCAGAAAACCTACTGTTGACAAGAGGAGTTGATGGCAGTTTTTTGGCAAGGCCTAGTAAAAGTAACCCTGGAGACTTCACACTTTCCGTTAGAAGAAATGGAGCTGTCACCCACATCAAGATTCAGAACACTGGTGATTACTATGACCTGTATGGAGGGGAGAAATTTGCCACTTTGGCTGAGTTGGTCCAGTATTACATGGAACATCACGGGCAATTAAAAGAGAAGAATGGAGATGTCATTGAGCTTAAATATCCTCTGAACTGTGCAGATCCTACCTCTGAAAGGTGGTTTCATGGACATCTCTCTGGGAAAGAAGCAGAGAAATTATTAACTGAAAAAGGAAAACATGGTAGTTTTCTTGTACGAGAGAGCCAGAGCCACCCTGGAGATTTTGTTCTTTCTGTGCGCACTGGTGATGACAAAGGGGAGAGCAATGACGGCAAGTCTAAAGTGACCCATGTTATGATTCGCTGTCAGGAACTGAAATACGACGTTGGTGGAGGAGAACGGTTTGATTCTTTGACAGATCTTGTGGAACATTATAAGAAGAATCCTATGGTGGAAACATTGGGTACAGTACTACAACTCAAGCAGCCCCTTAACACGACTCGTATAAATGCTGCTGAAATAGAAAGCAGAGTTCGAGAACTAAGCAAATTAGCTGAGACCACAGATAAAGTCAAACAAGGCTTTTGGGAAGAATTTGAGACACTACAACAACAGGAGTGCAAACTTCTCTACAGCCGAAAAGAGGGTCAAAGGCAAGAAAACAAAAACAAAAATAGATATAAAAACATCCTGCCCTTTGATCATACCAGGGTTGTCCTACACGATGGTGATCCCAATGAGCCTGTTTCAGATTACATCAATGCAAATATCATCATGCCTGAATTTGAAACCAAGTGCAACAATTCAAAGCCCAAAAAGAGTTACATTGCCACACAAGGCTGCCTGCAAAACACGGTGAATGACTTTTGGCGGATGGTGTTCCAAGAAAACTCCCGAGTGATTGTCATGACAACGAAAGAAGTGGAGAGAGGAAAGAGTAAATGTGTCAAATACTGGCCTGATGAGTATGCTCTAAAAGAATATGGCGTCATGCGTGTTAGGAACGTCAAAGAAAGCGCCGCTCATGACTATACGCTAAGAGAACTTAAACTTTCAAAGGTTGGACAAGGGAATACGGAGAGAACGGTCTGGCAATACCACTTTCGGACCTGGCCGGACCACGGCGTGCCCAGCGACCCTGGGGGCGTGCTGGACTTCCTGGAGGAGGTGCACCATAAGCAGGAGAGCATCATGGATGCAGGGCCGGTCGTGGTGCACTGCAGTGCTGGAATTGGCCGGACAGGGACGTTCATTGTGATTGATATTCTTATTGACATCATCAGAGAGAAAGGTGTTGACTGCGATATTGACGTTCCCAAAACCATCCAGATGGTGCGGTCTCAGAGGTCAGGGATGGTCCAGACAGAAGCACAGTACCGATTTATCTATATGGCGGTCCAGCATTATATTGAAACACTACAGCGCAGGATTGAAGAAGAGCAGAAAAGCAAGAGGAAAGGGCACGAATATACAAATATTAAGTATTCTCTAGCGGACCAGACGAGTGGAGATCAGAGCCCTCTCCCGCCTTGTACTCCAACGCCACCCTGTGCAGAAATGAGAGAAGACAGTGCTAGAGTCTATGAAAACGTGGGCCTGATGCAACAGCAGAAAAGTTTCAGATGAGAAAACCTGCCAAAACTTCAGCACAGAAATAGATGTGGACTTTCACCCTCTCCCTAAAAAGATCAAGAACAGACGCAAGAAAGTTTATGTGAAGACAGAATTTGGATTTGGAAGGCTTGCAATGTGGTTGACTACCTTTTGATAAGCAAAATTTGAAACCATTTAAAGACCACTGTATTTTAACTCAACAATACCTGCTTCCCAATTACTCATTTCCTCAGATAAGAAGAAATCATCTCTACAATGTAGACAACATTATATTTTATAGAATTTGTTTGAAATTGAGGAAGCAGTTAAATTGTGCGCTGTATTTTGCAGATTATGGGGATTCAAATTCTAGTAATAGGCTTTTTTATTTTTATTTTTATACCCTTAACCAGTTTAATTTTTTTTTTCCTCATTGTTGGGGATGATGAGAAGAAATGATTTGGGAAAATTAAGTAACAACGACCTAGAAAAGTGAGAACAATCTCATTTACCATCATGTATCCAGTAGTGGATAATTCATTTTGATGGCTTCTATTTTTGGCCAAATGAGAATTAAGCCAGTGCCTGAGACTGTCAGAAGTTGACCTTTGCACTGGCATTAAAGAGTCATAGAAAAAGAATCATGGATATTTATGAATTAAGGTAAGAGGTGTGGCTTTTTTTTTTTTCTTTTTTCCAGCCGTTGACCAATTATAGTTCGGCTGTTGACTGAGAAGTTTGTGGTGGGAAAACGTTTGCCATATTTTCTTTGCATTTGAATAATTGTCTTGTACTTAGAAAAAAGGCGTCTATGAATGACCAGTGTTTTTGGTCGCCAAATGTTGCTGACAAACTTATCCCAAAACTTTAGTGGCTTAAAAAAACCTGCCCCCAACTGTTAGTCAATCTGAGCTGGGCTCAGCTGGGCTGTTCTTCTGCCAGCCTGCAGGTGGCCACTCATGTGGTCAGCAGGTCGGCGGAGAGACTGGGATGGCTGGGCTTCTCTCTCTGCCTGCAGTCCTGAGTCTCTCCTTCTTCGTGTAGTCTCTTTCAGTGGCCTGGCTGGCAGGGTAGCTAGACCTCTCACATGCAGCTCAGAGCTCCCAAGAGCTCAAAAGCAGAAATGGCCAGGCCTTCTGAAAACTTAAGTCCAGAATTGTCACAGTGTCCCTTCTACTTCCCTCTATTGATGATGATGATGATGATGATGATGATGATGATGATGATGATGATGGTTTTTTCTAATCAGAAGAAAGCTGGGGTATGCCCTCTACTTACTAAACAAGTCACAAGCCCAGCTCAGATTCAAGAAAAGGGTGTGAAGTAGAGGTGCAGTTAAGTGGGGGGCCACTAGTCTAACAGACGGTCACAACCAGTGCCATGGAAAACCAAGGATATTAGCAAAAGCAGAAGTTGCTAGTGACCTTGGGAAGCCGAAGCTGCTTACAGTAGCTGGGACAAGCTGAAAGTCAGACTAAGAAATAAAGAGAGGGCCTTCAAGAAGCTTCCTGAATGATTTCTGCTAGCCCTGAGCCTATTTTTGGAACCAGCACTTGGGGAAACTGATCTTGTGAGGATGGATGTGTTTAGGGACACAGGGCTTTTGAGAGCAGCACCACCCCACTGGGGCATCCCCAGACTTGGGAAACGTGACTCTTTCTTAATGCCACTGGGTTTTAGTCAGGCCACAGTGAGAAGGAACAGCCCTAACAGGCCTCCAGCCAGGTTGAATGAGCTCATTTTTGTTGTAGCCAACCAGTAAGATTTGCTAATGTTCTACATTAAGTGCCTTCTCCAAAGACATCCCTCTTTGCCTCATATGTTGAATCATCCAGTGCGGATATTTCAATGAAAATATCATTGGTTGACTTTTGTGATGGTAATAATGCTATGGCATCTTTGCCATGAAGTTGTGGCCTCCTTGGATTCTTCTGACTTTGGCTTCTGAAAGGAAGGCCTAGATCCAGCCCTGGTGGTAGTTCCTTTCTGAGGTCTCTCAGTCCCTTGAGACTTTGGGGTAGTTTGGCTGCCATTCTCACTGACAAAATGTATATCAGCCCCCACCTCCACCCCCCAATATTCCTTGAACTTTGAATTGCTTCAGAACACAGGTGTGGCCTGAAGGTATTCCCTTATTAGGGAAGTGTCACTGCTGTCTTCTAGTCAAACTTGTAAAGAAAAAGATTCCAGTTCAGTATTTGCAGCAAGAAGCTTGAATGCTGTTCTTTTTATCGCATTGTTACATCGACTCATTCTCCATTTTGCTTTGGTTTTGTCTTGACTTGACTTGACTTTGGGGGTAAAGTCTTTCACCAGCACACAAGAGTTTGATTGTACAAATATATCTTCTGCATTAACATCTCTGCCTGTTGCTTAAGATCAGTTGCTTTTATACTCAGAATGGAAATACCTGATCTTGGCTAGTTTTGTTATAAGATATTGATTTCATTTAGATTTCCCTCCACGAGGTCAGCAAACTATCATGTTCTTATGTAAACTTAGGCCAAGGCCAGAGTTATCATAGTCCCTAGGTTGCTACGGCTTATCATGTGCTTGGTAAAAGGTGATCGCAGGTTCTCAGACGAGTTTACTTTACATGAGATGGAATCAGGCAGAGAGGCTGGGATGATGGAGAAAGCTCGAGGTGAAGTTTTAAAAAAAAAGTTGTGGAAAGGAAAGTTCCAAAGAGGTGGTTTCTGAGGAAGTCAGAGCGCCCAGGGCCAGAGCAGTCAGTAATGGGTGAATGAGGTTGTTTGGAAAGTCGGTGTGACAGACACATGGATGCCATCTACTTCTAGGTTGCTGGTGGGTATTAAATATGCACAATATTCCATAGCTCACTGAGGATTTTAAAATTATAAGCATAGGATTTTATATTTTGGGGTGAAAGAATTATCTGGCACATTAGGTATTGGAGTTTAAAAAAAAAGCCAAATTTCACAGTCTTAATAACTTTTTTTAAAAAAAACTAAAAGGCGCTTCATGTCCAGTGTGTGGCCCTTCTGAAACTTATGGTCATCTCTCCCACTGAAACCAAGGTCTTTTCAAATGTGGCTAAATGGGGATGAGGAGACACGGGTAGGACTTTCTTGGTGTGTGTGCATTCTTTAAAGAGCCAAGTTGCTTCGGGGAAACAGCCAGGAAAATGGTCAAGATTATTTTTAGAGGTTATTTTATTGGGGATTTTAAGAACTAATAACATCTTGAGTTATTTTTAATTCAGGGGGATGTGGAAAGGTTTGCAATTGTCAAGTGTTTTGTTGTAGCTTAGTATCCATAAGGGAAACTTAGACTATAGACATAACTACAAAGCCAGTGCAGCTTTTGTTTTCTGTATGTTGTTGGGGGATCAACTTTCACACATAGCAAGCACATGGCCTCCCTGATGTCAGGATGCCTTTGTTAGGATCTGTATTTGCCCTTAATTTTGTTGAAATCTTTTTTCCTTCTTCCTCTTGAAAAGTTCCAAAATATAGTTTATTGTATCTTTCATCACTAAAAATTTGTTCCTTTTTCACTATGGGCAGTTCACACAAGGCAAAAACTATTGAACAGTTGGTTTTAGTGTGTTGTATAACTTTGCTGTATATCAAACTAATTTTGACAAGTTTTCATCCTAAGCCTCAAATCATGTAATTAATAATTTGCCTGTTTATTTATGACCTAATTGTGATTCTTTTATTAATAAAAGCTAATGGGAAAAGGATCCCTGATTAAGCTGATGACTAGACCTACAATTAATTTTCCTGCAGTATATGAAGTATTGTACCAGAGTATTAAAAGATATGTAATATTTTATTGATAAATCTATCCTTTAAAAGGAATACGTTTTAGGATGTCATCATTTTGATGTGAATCATGTAAATGTTGATAATATGCTGTTTATTATACATTTAGTGTTTCAAGAGATTCACTTAATTGCCTTTTTGCCCACGTATATTATGTAGTCTATTTGCAACTGTTCTTAAAAAAATGACATTAAAAGAATAGTTTATGTAGAGAAACATTAGTGGATGTTAATTGTCTCCCCACCTATATTTATGGGTGTTAGCGCAACTGCTTTGCTAGTTGCAAAGCTGTATTATCAGAGTAAAAGTGTATTTGTAAACTGTATGGGAACTAAAAATTAGGAATAAAACCATTTTCTTATATGAAAAAAAAAAAAAAAAAA
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 71997, GC content failed 4553, low tm 36004, high tm 15874, high any compl 12, high end compl 59, high repeat similarity 81, long poly-x seq 325, ok 15089
PRIMER_RIGHT_EXPLAIN=considered 71995, GC content failed 4599, low tm 36555, high tm 15346, high any compl 5, high end compl 20, high repeat similarity 25, long poly-x seq 335, ok 15110
PRIMER_PAIR_EXPLAIN=considered 8606, unacceptable product size 8521, high any compl 2, high end compl 8, ok 75
PRIMER_PAIR_PENALTY=0.0434
PRIMER_LEFT_PENALTY=0.003218
PRIMER_RIGHT_PENALTY=0.040223
PRIMER_LEFT_SEQUENCE=ATTTGTTTGAAATTGAGGAAGCA
PRIMER_RIGHT_SEQUENCE=CTCAGATTGACTAACAGTTGGGG
PRIMER_LEFT=2431,23
PRIMER_RIGHT=3040,23
PRIMER_LEFT_TM=59.997
PRIMER_RIGHT_TM=60.040
PRIMER_LEFT_GC_PERCENT=30.435
PRIMER_RIGHT_GC_PERCENT=47.826
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_MISPRIMING_SCORE=12.00, L1ME2 3'-end of L1 repeat (subfamily L1ME2) - a consensus sequence
PRIMER_RIGHT_MISPRIMING_SCORE=11.00, reverse MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_PAIR_MISPRIMING_SCORE=20.00, L1ME2 3'-end of L1 repeat (subfamily L1ME2) - a consensus sequence
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=11.2000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=610
PRIMER_PAIR_PENALTY_1=0.0625
PRIMER_LEFT_1_PENALTY=0.006308
PRIMER_RIGHT_1_PENALTY=0.056142
PRIMER_LEFT_1_SEQUENCE=ATGGTTTCACCCAAATATCACTG
PRIMER_RIGHT_1_SEQUENCE=CTGCTTTCTATTTCAGCAGCATT
PRIMER_LEFT_1=394,23
PRIMER_RIGHT_1=1065,23
PRIMER_LEFT_1_TM=59.994
PRIMER_RIGHT_1_TM=60.056
PRIMER_LEFT_1_GC_PERCENT=39.130
PRIMER_RIGHT_1_GC_PERCENT=39.130
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_MISPRIMING_SCORE=12.00, MER4C a consensus
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_1_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=672
PRIMER_PAIR_PENALTY_2=0.0704
PRIMER_LEFT_2_PENALTY=0.014220
PRIMER_RIGHT_2_PENALTY=0.056142
PRIMER_LEFT_2_SEQUENCE=AGTATTACATGGAACATCACGGG
PRIMER_RIGHT_2_SEQUENCE=CTGCTTTCTATTTCAGCAGCATT
PRIMER_LEFT_2=615,23
PRIMER_RIGHT_2=1065,23
PRIMER_LEFT_2_TM=60.014
PRIMER_RIGHT_2_TM=60.056
PRIMER_LEFT_2_GC_PERCENT=43.478
PRIMER_RIGHT_2_GC_PERCENT=39.130
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_MISPRIMING_SCORE=11.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_2_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_2_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_2_END_STABILITY=11.1000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=451
PRIMER_PAIR_PENALTY_3=0.0756
PRIMER_LEFT_3_PENALTY=0.003218
PRIMER_RIGHT_3_PENALTY=0.072339
PRIMER_LEFT_3_SEQUENCE=ATTTGTTTGAAATTGAGGAAGCA
PRIMER_RIGHT_3_SEQUENCE=AGTAAGTAGAGGGCATACCCCAG
PRIMER_LEFT_3=2431,23
PRIMER_RIGHT_3=3407,23
PRIMER_LEFT_3_TM=59.997
PRIMER_RIGHT_3_TM=59.928
PRIMER_LEFT_3_GC_PERCENT=30.435
PRIMER_RIGHT_3_GC_PERCENT=52.174
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, L1ME2 3'-end of L1 repeat (subfamily L1ME2) - a consensus sequence
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_3_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=977
PRIMER_PAIR_PENALTY_4=0.0879
PRIMER_LEFT_4_PENALTY=0.006308
PRIMER_RIGHT_4_PENALTY=0.081604
PRIMER_LEFT_4_SEQUENCE=CATGGATGCCATCTACTTCTAGG
PRIMER_RIGHT_4_SEQUENCE=TTAAAGAATGCACACACACCAAG
PRIMER_LEFT_4=4905,23
PRIMER_RIGHT_4=5239,23
PRIMER_LEFT_4_TM=59.994
PRIMER_RIGHT_4_TM=60.082
PRIMER_LEFT_4_GC_PERCENT=47.826
PRIMER_RIGHT_4_GC_PERCENT=39.130
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_MISPRIMING_SCORE=10.00, L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_4_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=335
PRIMER_PAIR_PENALTY_5=0.0879
PRIMER_LEFT_5_PENALTY=0.006308
PRIMER_RIGHT_5_PENALTY=0.081604
PRIMER_LEFT_5_SEQUENCE=CATGGATGCCATCTACTTCTAGG
PRIMER_RIGHT_5_SEQUENCE=CTTTAAAGAATGCACACACACCA
PRIMER_LEFT_5=4905,23
PRIMER_RIGHT_5=5241,23
PRIMER_LEFT_5_TM=59.994
PRIMER_RIGHT_5_TM=60.082
PRIMER_LEFT_5_GC_PERCENT=47.826
PRIMER_RIGHT_5_GC_PERCENT=39.130
PRIMER_LEFT_5_SELF_ANY=7.00
PRIMER_RIGHT_5_SELF_ANY=8.00
PRIMER_LEFT_5_SELF_END=2.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_MISPRIMING_SCORE=10.00, L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_RIGHT_5_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_5_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_5_END_STABILITY=7.2000
PRIMER_RIGHT_5_END_STABILITY=8.2000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_5=337
PRIMER_PAIR_PENALTY_6=0.1006
PRIMER_LEFT_6_PENALTY=0.006308
PRIMER_RIGHT_6_PENALTY=0.094334
PRIMER_LEFT_6_SEQUENCE=CATGGATGCCATCTACTTCTAGG
PRIMER_RIGHT_6_SEQUENCE=ACTCCAATACCTAATGTGCCAGA
PRIMER_LEFT_6=4905,23
PRIMER_RIGHT_6=5046,23
PRIMER_LEFT_6_TM=59.994
PRIMER_RIGHT_6_TM=59.906
PRIMER_LEFT_6_GC_PERCENT=47.826
PRIMER_RIGHT_6_GC_PERCENT=43.478
PRIMER_LEFT_6_SELF_ANY=7.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=2.00
PRIMER_RIGHT_6_SELF_END=3.00
PRIMER_LEFT_6_MISPRIMING_SCORE=10.00, L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_RIGHT_6_MISPRIMING_SCORE=12.00, reverse MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_PAIR_6_MISPRIMING_SCORE=21.00, reverse MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_LEFT_6_END_STABILITY=7.2000
PRIMER_RIGHT_6_END_STABILITY=8.2000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_6=142
PRIMER_PAIR_PENALTY_7=0.1019
PRIMER_LEFT_7_PENALTY=0.006308
PRIMER_RIGHT_7_PENALTY=0.095601
PRIMER_LEFT_7_SEQUENCE=ATGGTTTCACCCAAATATCACTG
PRIMER_RIGHT_7_SEQUENCE=GCTCAATGACATCTCCATTCTTC
PRIMER_LEFT_7=394,23
PRIMER_RIGHT_7=671,23
PRIMER_LEFT_7_TM=59.994
PRIMER_RIGHT_7_TM=60.096
PRIMER_LEFT_7_GC_PERCENT=39.130
PRIMER_RIGHT_7_GC_PERCENT=43.478
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=3.00
PRIMER_RIGHT_7_SELF_END=2.00
PRIMER_LEFT_7_MISPRIMING_SCORE=12.00, MER4C a consensus
PRIMER_RIGHT_7_MISPRIMING_SCORE=12.00, MLT1d (MLT1d subfamily) - consensus sequence
PRIMER_PAIR_7_MISPRIMING_SCORE=21.00, MER4A a consensus
PRIMER_LEFT_7_END_STABILITY=6.7000
PRIMER_RIGHT_7_END_STABILITY=6.7000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_7=278
PRIMER_PAIR_PENALTY_8=0.1021
PRIMER_LEFT_8_PENALTY=0.020535
PRIMER_RIGHT_8_PENALTY=0.081604
PRIMER_LEFT_8_SEQUENCE=CTTTACATGAGATGGAATCAGGC
PRIMER_RIGHT_8_SEQUENCE=TTAAAGAATGCACACACACCAAG
PRIMER_LEFT_8=4719,23
PRIMER_RIGHT_8=5239,23
PRIMER_LEFT_8_TM=59.979
PRIMER_RIGHT_8_TM=60.082
PRIMER_LEFT_8_GC_PERCENT=43.478
PRIMER_RIGHT_8_GC_PERCENT=39.130
PRIMER_LEFT_8_SELF_ANY=5.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=2.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_8_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_8_END_STABILITY=9.7000
PRIMER_RIGHT_8_END_STABILITY=8.5000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_8=521
PRIMER_PAIR_PENALTY_9=0.1021
PRIMER_LEFT_9_PENALTY=0.020535
PRIMER_RIGHT_9_PENALTY=0.081604
PRIMER_LEFT_9_SEQUENCE=CTTTACATGAGATGGAATCAGGC
PRIMER_RIGHT_9_SEQUENCE=CTTTAAAGAATGCACACACACCA
PRIMER_LEFT_9=4719,23
PRIMER_RIGHT_9=5241,23
PRIMER_LEFT_9_TM=59.979
PRIMER_RIGHT_9_TM=60.082
PRIMER_LEFT_9_GC_PERCENT=43.478
PRIMER_RIGHT_9_GC_PERCENT=39.130
PRIMER_LEFT_9_SELF_ANY=5.00
PRIMER_RIGHT_9_SELF_ANY=8.00
PRIMER_LEFT_9_SELF_END=2.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_9_MISPRIMING_SCORE=22.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_9_END_STABILITY=9.7000
PRIMER_RIGHT_9_END_STABILITY=8.2000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_9=523
PRIMER_PAIR_PENALTY_10=0.1031
PRIMER_LEFT_10_PENALTY=0.014220
PRIMER_RIGHT_10_PENALTY=0.088859
PRIMER_LEFT_10_SEQUENCE=AGTATTACATGGAACATCACGGG
PRIMER_RIGHT_10_SEQUENCE=ATCAAAGGGCAGGATGTTTTTAT
PRIMER_LEFT_10=615,23
PRIMER_RIGHT_10=1237,23
PRIMER_LEFT_10_TM=60.014
PRIMER_RIGHT_10_TM=60.089
PRIMER_LEFT_10_GC_PERCENT=43.478
PRIMER_RIGHT_10_GC_PERCENT=34.783
PRIMER_LEFT_10_SELF_ANY=6.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=2.00
PRIMER_RIGHT_10_SELF_END=2.00
PRIMER_LEFT_10_MISPRIMING_SCORE=11.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_10_MISPRIMING_SCORE=11.00, MSTc consensus sequence
PRIMER_PAIR_10_MISPRIMING_SCORE=20.00, MSTc consensus sequence
PRIMER_LEFT_10_END_STABILITY=11.1000
PRIMER_RIGHT_10_END_STABILITY=6.2000
PRIMER_PAIR_10_COMPL_ANY=6.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_10=623
PRIMER_PAIR_PENALTY_11=0.1045
PRIMER_LEFT_11_PENALTY=0.040223
PRIMER_RIGHT_11_PENALTY=0.064245
PRIMER_LEFT_11_SEQUENCE=CCCCAACTGTTAGTCAATCTGAG
PRIMER_RIGHT_11_SEQUENCE=TAGTCTGACTTTCAGCTTGTCCC
PRIMER_LEFT_11=3018,23
PRIMER_RIGHT_11=3605,23
PRIMER_LEFT_11_TM=60.040
PRIMER_RIGHT_11_TM=59.936
PRIMER_LEFT_11_GC_PERCENT=47.826
PRIMER_RIGHT_11_GC_PERCENT=47.826
PRIMER_LEFT_11_SELF_ANY=5.00
PRIMER_RIGHT_11_SELF_ANY=7.00
PRIMER_LEFT_11_SELF_END=3.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, MER4A a consensus
PRIMER_PAIR_11_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_11_END_STABILITY=6.7000
PRIMER_RIGHT_11_END_STABILITY=9.1000
PRIMER_PAIR_11_COMPL_ANY=7.00
PRIMER_PAIR_11_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_11=588
PRIMER_PAIR_PENALTY_12=0.1108
PRIMER_LEFT_12_PENALTY=0.072339
PRIMER_RIGHT_12_PENALTY=0.038458
PRIMER_LEFT_12_SEQUENCE=CTGGGGTATGCCCTCTACTTACT
PRIMER_RIGHT_12_SEQUENCE=CAAAGTCTCAAGGGACTGAGAGA
PRIMER_LEFT_12=3385,23
PRIMER_RIGHT_12=4143,23
PRIMER_LEFT_12_TM=59.928
PRIMER_RIGHT_12_TM=60.038
PRIMER_LEFT_12_GC_PERCENT=52.174
PRIMER_RIGHT_12_GC_PERCENT=47.826
PRIMER_LEFT_12_SELF_ANY=6.00
PRIMER_RIGHT_12_SELF_ANY=6.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=3.00
PRIMER_LEFT_12_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_12_MISPRIMING_SCORE=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_PAIR_12_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_12_END_STABILITY=5.7000
PRIMER_RIGHT_12_END_STABILITY=6.4000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_12=759
PRIMER_PAIR_PENALTY_13=0.1118
PRIMER_LEFT_13_PENALTY=0.047559
PRIMER_RIGHT_13_PENALTY=0.064245
PRIMER_LEFT_13_SEQUENCE=ACAGTGTCCCTTCTACTTCCCTC
PRIMER_RIGHT_13_SEQUENCE=TAGTCTGACTTTCAGCTTGTCCC
PRIMER_LEFT_13=3290,23
PRIMER_RIGHT_13=3605,23
PRIMER_LEFT_13_TM=60.048
PRIMER_RIGHT_13_TM=59.936
PRIMER_LEFT_13_GC_PERCENT=52.174
PRIMER_RIGHT_13_GC_PERCENT=47.826
PRIMER_LEFT_13_SELF_ANY=5.00
PRIMER_RIGHT_13_SELF_ANY=7.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_13_MISPRIMING_SCORE=12.00, MER4A a consensus
PRIMER_PAIR_13_MISPRIMING_SCORE=22.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_LEFT_13_END_STABILITY=9.4000
PRIMER_RIGHT_13_END_STABILITY=9.1000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_13=316
PRIMER_PAIR_PENALTY_14=0.1126
PRIMER_LEFT_14_PENALTY=0.040223
PRIMER_RIGHT_14_PENALTY=0.072339
PRIMER_LEFT_14_SEQUENCE=CCCCAACTGTTAGTCAATCTGAG
PRIMER_RIGHT_14_SEQUENCE=AGTAAGTAGAGGGCATACCCCAG
PRIMER_LEFT_14=3018,23
PRIMER_RIGHT_14=3407,23
PRIMER_LEFT_14_TM=60.040
PRIMER_RIGHT_14_TM=59.928
PRIMER_LEFT_14_GC_PERCENT=47.826
PRIMER_RIGHT_14_GC_PERCENT=52.174
PRIMER_LEFT_14_SELF_ANY=5.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=3.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_14_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_14_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_14_END_STABILITY=6.7000
PRIMER_RIGHT_14_END_STABILITY=9.7000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_14=390
PRIMER_PAIR_PENALTY_15=0.1150
PRIMER_LEFT_15_PENALTY=0.047559
PRIMER_RIGHT_15_PENALTY=0.067430
PRIMER_LEFT_15_SEQUENCE=ACAGTGTCCCTTCTACTTCCCTC
PRIMER_RIGHT_15_SEQUENCE=AGTCTCAAGGGACTGAGAGACCT
PRIMER_LEFT_15=3290,23
PRIMER_RIGHT_15=4140,23
PRIMER_LEFT_15_TM=60.048
PRIMER_RIGHT_15_TM=59.933
PRIMER_LEFT_15_GC_PERCENT=52.174
PRIMER_RIGHT_15_GC_PERCENT=52.174
PRIMER_LEFT_15_SELF_ANY=5.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=3.00
PRIMER_LEFT_15_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_15_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_15_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_15_END_STABILITY=9.4000
PRIMER_RIGHT_15_END_STABILITY=7.6000
PRIMER_PAIR_15_COMPL_ANY=7.00
PRIMER_PAIR_15_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_15=851
PRIMER_PAIR_PENALTY_16=0.1203
PRIMER_LEFT_16_PENALTY=0.038744
PRIMER_RIGHT_16_PENALTY=0.081604
PRIMER_LEFT_16_SEQUENCE=CCTGTTGCTTAAGATCAGTTGCT
PRIMER_RIGHT_16_SEQUENCE=TTAAAGAATGCACACACACCAAG
PRIMER_LEFT_16=4494,23
PRIMER_RIGHT_16=5239,23
PRIMER_LEFT_16_TM=59.961
PRIMER_RIGHT_16_TM=60.082
PRIMER_LEFT_16_GC_PERCENT=43.478
PRIMER_RIGHT_16_GC_PERCENT=39.130
PRIMER_LEFT_16_SELF_ANY=8.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=2.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_MISPRIMING_SCORE=12.00, LTR8 region
PRIMER_RIGHT_16_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_16_MISPRIMING_SCORE=22.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_16_END_STABILITY=8.5000
PRIMER_RIGHT_16_END_STABILITY=8.5000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_16=746
PRIMER_PAIR_PENALTY_17=0.1203
PRIMER_LEFT_17_PENALTY=0.038744
PRIMER_RIGHT_17_PENALTY=0.081604
PRIMER_LEFT_17_SEQUENCE=CCTGTTGCTTAAGATCAGTTGCT
PRIMER_RIGHT_17_SEQUENCE=CTTTAAAGAATGCACACACACCA
PRIMER_LEFT_17=4494,23
PRIMER_RIGHT_17=5241,23
PRIMER_LEFT_17_TM=59.961
PRIMER_RIGHT_17_TM=60.082
PRIMER_LEFT_17_GC_PERCENT=43.478
PRIMER_RIGHT_17_GC_PERCENT=39.130
PRIMER_LEFT_17_SELF_ANY=8.00
PRIMER_RIGHT_17_SELF_ANY=8.00
PRIMER_LEFT_17_SELF_END=2.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_MISPRIMING_SCORE=12.00, LTR8 region
PRIMER_RIGHT_17_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_17_MISPRIMING_SCORE=23.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_17_END_STABILITY=8.5000
PRIMER_RIGHT_17_END_STABILITY=8.2000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_17=748
PRIMER_PAIR_PENALTY_18=0.1287
PRIMER_LEFT_18_PENALTY=0.088469
PRIMER_RIGHT_18_PENALTY=0.040223
PRIMER_LEFT_18_SEQUENCE=GACCAATTATAGTTCGGCTGTTG
PRIMER_RIGHT_18_SEQUENCE=CTCAGATTGACTAACAGTTGGGG
PRIMER_LEFT_18=2834,23
PRIMER_RIGHT_18=3040,23
PRIMER_LEFT_18_TM=59.912
PRIMER_RIGHT_18_TM=60.040
PRIMER_LEFT_18_GC_PERCENT=43.478
PRIMER_RIGHT_18_GC_PERCENT=47.826
PRIMER_LEFT_18_SELF_ANY=6.00
PRIMER_RIGHT_18_SELF_ANY=5.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_18_MISPRIMING_SCORE=11.00, reverse MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_PAIR_18_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_18_END_STABILITY=7.0000
PRIMER_RIGHT_18_END_STABILITY=11.2000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_18=207
PRIMER_PAIR_PENALTY_19=0.1287
PRIMER_LEFT_19_PENALTY=0.088469
PRIMER_RIGHT_19_PENALTY=0.040223
PRIMER_LEFT_19_SEQUENCE=TTGACCAATTATAGTTCGGCTGT
PRIMER_RIGHT_19_SEQUENCE=CTCAGATTGACTAACAGTTGGGG
PRIMER_LEFT_19=2832,23
PRIMER_RIGHT_19=3040,23
PRIMER_LEFT_19_TM=59.912
PRIMER_RIGHT_19_TM=60.040
PRIMER_LEFT_19_GC_PERCENT=39.130
PRIMER_RIGHT_19_GC_PERCENT=47.826
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=5.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_19_MISPRIMING_SCORE=11.00, reverse MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_PAIR_19_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_19_END_STABILITY=7.9000
PRIMER_RIGHT_19_END_STABILITY=11.2000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_19=209
=