PRIMER_SEQUENCE_ID=NM_002835
SEQUENCE=AGCGACCGCAGCCGGGGGGACGCGGGAGGATGGAGCAAGTGGAGATCCTGAGGAAATTCATCCAGAGGGTCCAGGCCATGAAGAGTCCTGACCACAATGGGGAGGACAACTTCGCCCGGGACTTCATGCGGTTAAGAAGATTGTCTACCAAATATAGAACAGAAAAGATATATCCCACAGCCACTGGAGAAAAAGAAGAAAATGTTAAAAAGAACAGATACAAGGACATACTGCCATTTGATCACAGCCGAGTTAAATTGACATTAAAGACTCCTTCACAAGATTCAGACTATATCAATGCAAATTTTATAAAGGGCGTCTATGGGCCAAAAGCATATGTAGCAACTCAAGGACCTTTAGCAAATACAGTAATAGATTTTTGGAGGATGATATGGGAGTATAATGTTGTGATCATTGTAATGGCCTGCCGAGAATTTGAGATGGGAAGGAAAAAATGTGAGCGCTATTGGCCTTTGTATGGAGAAGACCCCATAACGTTTGCACCATTTAAAATTTCTTGTGAGGATGAACAAGCAAGAACAGACTACTTCATCAGGACACTCTTACTTGAATTTCAAAATGAATCTCGTAGGCTGTATCAGTTTCATTATGTGAACTGGCCAGACCATGATGTTCCTTCATCATTTGATTCTATTCTGGACATGATAAGCTTAATGAGGAAATATCAAGAACATGAAGATGTTCCTATTTGTATTCATTGCAGTGCAGGCTGTGGAAGAACAGGTGCCATTTGTGCCATAGATTATACGTGGAATTTACTAAAAGCTGGGAAAATACCAGAGGAATTTAATGTATTTAATTTAATACAAGAAATGAGAACACAAAGGCATTCTGCAGTACAAACAAAGGAGCAATATGAACTTGTTCATAGAGCTATTGCCCAACTGTTTGAAAAACAGCTACAACTATATGAAATTCATGGAGCTCAGAAAATTGCTGATGGAGTGAATGAAATTAACACTGAAAACATGATCAGCTCCATAGAGCCTGAAAAACAAGATTCTCCTCCTCCAAAACCACCAAGGACCCGCAGTTGCCTTGTTGAAGGGGATGCTAAAGAAGAAATACTGCAGCCACCGGAACCTCATCCAGTGCCACCCATCTTGACACCTTCTCCCCCTTCAGCTTTTCCAACAGTCACTACTGTGTGGCAGGACAATGATAGATACCATCCAAAGCCAGTGTTGCATATGGTTTCATCAGAACAACATTCAGCAGACCTCAACAGAAACTATAGTAAATCAACAGAACTTCCAGGGAAAAATGAATCAACAATTGAACAGATAGATAAAAAATTGGAACGAAATTTAAGTTTTGAGATTAAGAAGGTCCCTCTCCAAGAGGGACCAAAAAGTTTTGATGGGAACACACTTTTGAATAGGGGACATGCAATTAAAATTAAATCTGCTTCACCTTGTATAGCTGATAAAATCTCTAAGCCACAGGAATTAAGTTCAGATCTAAATGTCGGTGATACTTCCCAGAATTCTTGTGTGGACTGCAGTGTAACACAATCAAACAAAGTTTCAGTTACTCCACCAGAAGAATCCCAGAATTCAGACACACCTCCAAGGCCAGACCGCTTGCCTCTTGATGAGAAAGGACATGTAACGTGGTCATTTCATGGACCTGAAAATGCCATACCCATACCTGATTTATCTGAAGGCAATTCCTCAGATATCAACTATCAAACTAGGAAAACTGTGAGTTTAACACCAAGTCCTACAACACAAGTTGAAACACCTGATCTTGTGGATCATGATAACACTTCACCACTCTTCAGAACACCCCTCAGTTTTACTAATCCACTTCACTCTGATGACTCAGACTCAGATGAAAGAAACTCTGATGGTGCTGTGACCCAGAATAAAACTAATATTTCAACAGCAAGTGCCACAGTTTCTGCTGCCACTAGTACTGAAAGCATTTCTACTAGGAAAGTATTGCCAATGTCCATTGCTAGACATAATATAGCAGGAACAACACATTCAGGTGCTGAAAAAGATGTTGATGTTAGTGAAGATTCACCTCCTCCCCTACCTGAAAGAACTCCTGAATCGTTTGTGTTAGCAAGTGAACATAATACACCTGTAAGATCGGAATGGAGTGAACTTCAAAGTCAGGAACGATCTGAACAAAAAAAGTCTGAAGGCTTGATAACCTCTGAAAATGAGAAATGTGATCATCCAGCGGGAGGTATTCACTATGAAATGTGCATAGAATGTCCACCTACTTTCAGTGACAAGAGAGAACAAATATCAGAAAATCCAACAGAAGCCACAGATATTGGTTTTGGTAATCGATGTGGAAAACCCAAAGGACCAAGAGATCCACCTTCAGAATGGACATGATTCAGGGAGCTAGAAGACACTTTAAGTTATACTGGAAAATTCAGGTGCCACTGAAAGCCAGATTTATAGTATTCCATCTTTAATATGTGGGACTAACAGCAGTGTAGATTGTTACCTTAATATTTTTTGCTGGGACCATCTACCTGCCTTATACTACACTTAGGAAAAAGTATTACATATGGTTTATTTTGAAACTTCAAGTATTATTGCCTTAATGTCTCTTAACCCTGTTACACGCTGCTTGTAGACATGTTAATATAGTAATACCTTTATGATATATTGAGTTTAAGGACTACTCTTTTTCTGTTTTATCATGTATGCATTATTTTGTATATGTACAGGGCAAGTAGGTATATAATTTGATAAAGTTGCAATTGAAATATTATTAACAGAAGATGTAAGAAATTTCTGCATGGTCTAAATCTTTGTGTACTTTATTTGTAAATTATTTGCCCTGGAGTTTTAGAAAATAGTTTCTGAATTTTAAACTTGCTGGATTCATGCAGCCAGCTTTGCAGGTTATCAGAGATCAAAGATTGTAATAATAATTTTGTAAATTGTAAGCAAAAAGTTATTTTTATATTATATACAGTCTAATTGTTCATCCTAATTGTTCCTGTTTTCATCTAGTCAGAGATTCAGTAAGTGCCTTGGAACAATATTGAATTCTCTTAGCTTGTGTGTGTTTCTTTAATATTTGAACTCAAGTGGGATTAGAAGACTATCAAAATACATGTATGTTTCAGGATATTTGACCTGTCATTAAAAAAAACAAACAGTTTTACAGTG
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 36067, GC content failed 2099, low tm 21823, high tm 4730, high any compl 10, high end compl 59, high repeat similarity 58, long poly-x seq 94, ok 7194
PRIMER_RIGHT_EXPLAIN=considered 35974, GC content failed 2042, low tm 22231, high tm 4375, high any compl 5, high end compl 11, high repeat similarity 12, long poly-x seq 106, ok 7192
PRIMER_PAIR_EXPLAIN=considered 1002, unacceptable product size 937, high end compl 6, ok 59
PRIMER_PAIR_PENALTY=0.0540
PRIMER_LEFT_PENALTY=0.005048
PRIMER_RIGHT_PENALTY=0.048962
PRIMER_LEFT_SEQUENCE=ACAAGATTCTCCTCCTCCAAAAC
PRIMER_RIGHT_SEQUENCE=TCAATTGTTGATTCATTTTTCCC
PRIMER_LEFT=1015,23
PRIMER_RIGHT=1299,23
PRIMER_LEFT_TM=59.995
PRIMER_RIGHT_TM=60.049
PRIMER_LEFT_GC_PERCENT=43.478
PRIMER_RIGHT_GC_PERCENT=30.435
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_MISPRIMING_SCORE=12.00, MSTc consensus sequence
PRIMER_RIGHT_MISPRIMING_SCORE=11.00, MER6 Nonautonomous DNA transposon
PRIMER_PAIR_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=285
PRIMER_PAIR_PENALTY_1=0.0626
PRIMER_LEFT_1_PENALTY=0.014220
PRIMER_RIGHT_1_PENALTY=0.048411
PRIMER_LEFT_1_SEQUENCE=AAATCCAACAGAAGCCACAGATA
PRIMER_RIGHT_1_SEQUENCE=ATTGTTCCAAGGCACTTACTGAA
PRIMER_LEFT_1=2284,23
PRIMER_RIGHT_1=3031,23
PRIMER_LEFT_1_TM=60.014
PRIMER_RIGHT_1_TM=60.048
PRIMER_LEFT_1_GC_PERCENT=39.130
PRIMER_RIGHT_1_GC_PERCENT=39.130
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, MER6 Nonautonomous DNA transposon
PRIMER_PAIR_1_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=748
PRIMER_PAIR_PENALTY_2=0.0626
PRIMER_LEFT_2_PENALTY=0.014220
PRIMER_RIGHT_2_PENALTY=0.048411
PRIMER_LEFT_2_SEQUENCE=AAATCCAACAGAAGCCACAGATA
PRIMER_RIGHT_2_SEQUENCE=TTGTTCCAAGGCACTTACTGAAT
PRIMER_LEFT_2=2284,23
PRIMER_RIGHT_2=3030,23
PRIMER_LEFT_2_TM=60.014
PRIMER_RIGHT_2_TM=60.048
PRIMER_LEFT_2_GC_PERCENT=39.130
PRIMER_RIGHT_2_GC_PERCENT=39.130
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_2_MISPRIMING_SCORE=12.00, MER6 Nonautonomous DNA transposon
PRIMER_PAIR_2_MISPRIMING_SCORE=22.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_2_END_STABILITY=5.6000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=747
PRIMER_PAIR_PENALTY_3=0.0673
PRIMER_LEFT_3_PENALTY=0.018344
PRIMER_RIGHT_3_PENALTY=0.048962
PRIMER_LEFT_3_SEQUENCE=ACTGAAAACATGATCAGCTCCAT
PRIMER_RIGHT_3_SEQUENCE=TCAATTGTTGATTCATTTTTCCC
PRIMER_LEFT_3=980,23
PRIMER_RIGHT_3=1299,23
PRIMER_LEFT_3_TM=60.018
PRIMER_RIGHT_3_TM=60.049
PRIMER_LEFT_3_GC_PERCENT=39.130
PRIMER_RIGHT_3_GC_PERCENT=30.435
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_MISPRIMING_SCORE=11.00, L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_RIGHT_3_MISPRIMING_SCORE=11.00, MER6 Nonautonomous DNA transposon
PRIMER_PAIR_3_MISPRIMING_SCORE=22.00, reverse MER7B Nonautonomous DNA transposon
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=8.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=320
PRIMER_PAIR_PENALTY_4=0.0842
PRIMER_LEFT_4_PENALTY=0.005048
PRIMER_RIGHT_4_PENALTY=0.079168
PRIMER_LEFT_4_SEQUENCE=ACAAGATTCTCCTCCTCCAAAAC
PRIMER_RIGHT_4_SEQUENCE=GACCACGTTACATGTCCTTTCTC
PRIMER_LEFT_4=1015,23
PRIMER_RIGHT_4=1638,23
PRIMER_LEFT_4_TM=59.995
PRIMER_RIGHT_4_TM=59.921
PRIMER_LEFT_4_GC_PERCENT=43.478
PRIMER_RIGHT_4_GC_PERCENT=47.826
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, MSTc consensus sequence
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_4_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=624
PRIMER_PAIR_PENALTY_5=0.0870
PRIMER_LEFT_5_PENALTY=0.005048
PRIMER_RIGHT_5_PENALTY=0.081990
PRIMER_LEFT_5_SEQUENCE=ACAAGATTCTCCTCCTCCAAAAC
PRIMER_RIGHT_5_SEQUENCE=GGAGAGGGACCTTCTTAATCTCA
PRIMER_LEFT_5=1015,23
PRIMER_RIGHT_5=1358,23
PRIMER_LEFT_5_TM=59.995
PRIMER_RIGHT_5_TM=60.082
PRIMER_LEFT_5_GC_PERCENT=43.478
PRIMER_RIGHT_5_GC_PERCENT=47.826
PRIMER_LEFT_5_SELF_ANY=5.00
PRIMER_RIGHT_5_SELF_ANY=7.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_MISPRIMING_SCORE=12.00, MSTc consensus sequence
PRIMER_RIGHT_5_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_5_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_5_END_STABILITY=7.0000
PRIMER_RIGHT_5_END_STABILITY=6.7000
PRIMER_PAIR_5_COMPL_ANY=6.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_5=344
PRIMER_PAIR_PENALTY_6=0.0870
PRIMER_LEFT_6_PENALTY=0.005048
PRIMER_RIGHT_6_PENALTY=0.081990
PRIMER_LEFT_6_SEQUENCE=ACAAGATTCTCCTCCTCCAAAAC
PRIMER_RIGHT_6_SEQUENCE=TGGAGAGGGACCTTCTTAATCTC
PRIMER_LEFT_6=1015,23
PRIMER_RIGHT_6=1359,23
PRIMER_LEFT_6_TM=59.995
PRIMER_RIGHT_6_TM=60.082
PRIMER_LEFT_6_GC_PERCENT=43.478
PRIMER_RIGHT_6_GC_PERCENT=47.826
PRIMER_LEFT_6_SELF_ANY=5.00
PRIMER_RIGHT_6_SELF_ANY=7.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_MISPRIMING_SCORE=12.00, MSTc consensus sequence
PRIMER_RIGHT_6_MISPRIMING_SCORE=12.00, MER22 Human SSTI moderately repetitive DNA sequence family
PRIMER_PAIR_6_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_6_END_STABILITY=7.0000
PRIMER_RIGHT_6_END_STABILITY=6.3000
PRIMER_PAIR_6_COMPL_ANY=7.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_6=345
PRIMER_PAIR_PENALTY_7=0.0975
PRIMER_LEFT_7_PENALTY=0.018344
PRIMER_RIGHT_7_PENALTY=0.079168
PRIMER_LEFT_7_SEQUENCE=ACTGAAAACATGATCAGCTCCAT
PRIMER_RIGHT_7_SEQUENCE=GACCACGTTACATGTCCTTTCTC
PRIMER_LEFT_7=980,23
PRIMER_RIGHT_7=1638,23
PRIMER_LEFT_7_TM=60.018
PRIMER_RIGHT_7_TM=59.921
PRIMER_LEFT_7_GC_PERCENT=39.130
PRIMER_RIGHT_7_GC_PERCENT=47.826
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=3.00
PRIMER_RIGHT_7_SELF_END=2.00
PRIMER_LEFT_7_MISPRIMING_SCORE=11.00, L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_RIGHT_7_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_7_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_7_END_STABILITY=8.1000
PRIMER_RIGHT_7_END_STABILITY=6.7000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_7=659
PRIMER_PAIR_PENALTY_8=0.1003
PRIMER_LEFT_8_PENALTY=0.018344
PRIMER_RIGHT_8_PENALTY=0.081990
PRIMER_LEFT_8_SEQUENCE=ACTGAAAACATGATCAGCTCCAT
PRIMER_RIGHT_8_SEQUENCE=GGAGAGGGACCTTCTTAATCTCA
PRIMER_LEFT_8=980,23
PRIMER_RIGHT_8=1358,23
PRIMER_LEFT_8_TM=60.018
PRIMER_RIGHT_8_TM=60.082
PRIMER_LEFT_8_GC_PERCENT=39.130
PRIMER_RIGHT_8_GC_PERCENT=47.826
PRIMER_LEFT_8_SELF_ANY=6.00
PRIMER_RIGHT_8_SELF_ANY=7.00
PRIMER_LEFT_8_SELF_END=3.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_MISPRIMING_SCORE=11.00, L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_8_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_8_END_STABILITY=8.1000
PRIMER_RIGHT_8_END_STABILITY=6.7000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_8=379
PRIMER_PAIR_PENALTY_9=0.1003
PRIMER_LEFT_9_PENALTY=0.018344
PRIMER_RIGHT_9_PENALTY=0.081990
PRIMER_LEFT_9_SEQUENCE=ACTGAAAACATGATCAGCTCCAT
PRIMER_RIGHT_9_SEQUENCE=TGGAGAGGGACCTTCTTAATCTC
PRIMER_LEFT_9=980,23
PRIMER_RIGHT_9=1359,23
PRIMER_LEFT_9_TM=60.018
PRIMER_RIGHT_9_TM=60.082
PRIMER_LEFT_9_GC_PERCENT=39.130
PRIMER_RIGHT_9_GC_PERCENT=47.826
PRIMER_LEFT_9_SELF_ANY=6.00
PRIMER_RIGHT_9_SELF_ANY=7.00
PRIMER_LEFT_9_SELF_END=3.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_MISPRIMING_SCORE=11.00, L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, MER22 Human SSTI moderately repetitive DNA sequence family
PRIMER_PAIR_9_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_9_END_STABILITY=8.1000
PRIMER_RIGHT_9_END_STABILITY=6.3000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_9=380
PRIMER_PAIR_PENALTY_10=0.1075
PRIMER_LEFT_10_PENALTY=0.102474
PRIMER_RIGHT_10_PENALTY=0.005048
PRIMER_LEFT_10_SEQUENCE=CTATGGGCCAAAAGCATATGTAG
PRIMER_RIGHT_10_SEQUENCE=GTTTTGGAGGAGGAGAATCTTGT
PRIMER_LEFT_10=319,23
PRIMER_RIGHT_10=1037,23
PRIMER_LEFT_10_TM=59.898
PRIMER_RIGHT_10_TM=59.995
PRIMER_LEFT_10_GC_PERCENT=43.478
PRIMER_RIGHT_10_GC_PERCENT=43.478
PRIMER_LEFT_10_SELF_ANY=6.00
PRIMER_RIGHT_10_SELF_ANY=5.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=3.00
PRIMER_LEFT_10_MISPRIMING_SCORE=12.00, L1PA11 3'-end of L1 repeat (subfamily L1PA11) - a consensus sequence
PRIMER_RIGHT_10_MISPRIMING_SCORE=12.00, reverse MER4C a consensus
PRIMER_PAIR_10_MISPRIMING_SCORE=21.00, reverse MER4C a consensus
PRIMER_LEFT_10_END_STABILITY=5.7000
PRIMER_RIGHT_10_END_STABILITY=6.7000
PRIMER_PAIR_10_COMPL_ANY=6.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_10=719
PRIMER_PAIR_PENALTY_11=0.1119
PRIMER_LEFT_11_PENALTY=0.106852
PRIMER_RIGHT_11_PENALTY=0.005048
PRIMER_LEFT_11_SEQUENCE=AATGCAAATTTTATAAAGGGCGT
PRIMER_RIGHT_11_SEQUENCE=GTTTTGGAGGAGGAGAATCTTGT
PRIMER_LEFT_11=296,23
PRIMER_RIGHT_11=1037,23
PRIMER_LEFT_11_TM=60.107
PRIMER_RIGHT_11_TM=59.995
PRIMER_LEFT_11_GC_PERCENT=30.435
PRIMER_RIGHT_11_GC_PERCENT=43.478
PRIMER_LEFT_11_SELF_ANY=8.00
PRIMER_RIGHT_11_SELF_ANY=5.00
PRIMER_LEFT_11_SELF_END=2.00
PRIMER_RIGHT_11_SELF_END=3.00
PRIMER_LEFT_11_MISPRIMING_SCORE=11.00, L1MD1 3'-end of L1 repeat (subfamily L1MD1) - a consensus sequence
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, reverse MER4C a consensus
PRIMER_PAIR_11_MISPRIMING_SCORE=20.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_11_END_STABILITY=11.1000
PRIMER_RIGHT_11_END_STABILITY=6.7000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_11=742
PRIMER_PAIR_PENALTY_12=0.1203
PRIMER_LEFT_12_PENALTY=0.012045
PRIMER_RIGHT_12_PENALTY=0.108280
PRIMER_LEFT_12_SEQUENCE=GCTGCCACTAGTACTGAAAGCAT
PRIMER_RIGHT_12_SEQUENCE=GTATAAGGCAGGTAGATGGTCCC
PRIMER_LEFT_12=1925,23
PRIMER_RIGHT_12=2525,23
PRIMER_LEFT_12_TM=59.988
PRIMER_RIGHT_12_TM=60.108
PRIMER_LEFT_12_GC_PERCENT=47.826
PRIMER_RIGHT_12_GC_PERCENT=52.174
PRIMER_LEFT_12_SELF_ANY=7.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=2.00
PRIMER_RIGHT_12_SELF_END=2.00
PRIMER_LEFT_12_MISPRIMING_SCORE=12.00, reverse MER7B Nonautonomous DNA transposon
PRIMER_RIGHT_12_MISPRIMING_SCORE=11.00, LTR13 LTR from a  putative human endogenous retrovirus
PRIMER_PAIR_12_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_12_END_STABILITY=8.1000
PRIMER_RIGHT_12_END_STABILITY=9.1000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_12=601
PRIMER_PAIR_PENALTY_13=0.1208
PRIMER_LEFT_13_PENALTY=0.102474
PRIMER_RIGHT_13_PENALTY=0.018344
PRIMER_LEFT_13_SEQUENCE=CTATGGGCCAAAAGCATATGTAG
PRIMER_RIGHT_13_SEQUENCE=ATGGAGCTGATCATGTTTTCAGT
PRIMER_LEFT_13=319,23
PRIMER_RIGHT_13=1002,23
PRIMER_LEFT_13_TM=59.898
PRIMER_RIGHT_13_TM=60.018
PRIMER_LEFT_13_GC_PERCENT=43.478
PRIMER_RIGHT_13_GC_PERCENT=39.130
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=6.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=2.00
PRIMER_LEFT_13_MISPRIMING_SCORE=12.00, L1PA11 3'-end of L1 repeat (subfamily L1PA11) - a consensus sequence
PRIMER_RIGHT_13_MISPRIMING_SCORE=12.00, MER34 a consensus
PRIMER_PAIR_13_MISPRIMING_SCORE=21.00, L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence
PRIMER_LEFT_13_END_STABILITY=5.7000
PRIMER_RIGHT_13_END_STABILITY=6.4000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_13=684
PRIMER_PAIR_PENALTY_14=0.1225
PRIMER_LEFT_14_PENALTY=0.014220
PRIMER_RIGHT_14_PENALTY=0.108280
PRIMER_LEFT_14_SEQUENCE=AAATCCAACAGAAGCCACAGATA
PRIMER_RIGHT_14_SEQUENCE=GTATAAGGCAGGTAGATGGTCCC
PRIMER_LEFT_14=2284,23
PRIMER_RIGHT_14=2525,23
PRIMER_LEFT_14_TM=60.014
PRIMER_RIGHT_14_TM=60.108
PRIMER_LEFT_14_GC_PERCENT=39.130
PRIMER_RIGHT_14_GC_PERCENT=52.174
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=2.00
PRIMER_RIGHT_14_SELF_END=2.00
PRIMER_LEFT_14_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_14_MISPRIMING_SCORE=11.00, LTR13 LTR from a  putative human endogenous retrovirus
PRIMER_PAIR_14_MISPRIMING_SCORE=21.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_14_END_STABILITY=5.6000
PRIMER_RIGHT_14_END_STABILITY=9.1000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_14=242
PRIMER_PAIR_PENALTY_15=0.1252
PRIMER_LEFT_15_PENALTY=0.106852
PRIMER_RIGHT_15_PENALTY=0.018344
PRIMER_LEFT_15_SEQUENCE=AATGCAAATTTTATAAAGGGCGT
PRIMER_RIGHT_15_SEQUENCE=ATGGAGCTGATCATGTTTTCAGT
PRIMER_LEFT_15=296,23
PRIMER_RIGHT_15=1002,23
PRIMER_LEFT_15_TM=60.107
PRIMER_RIGHT_15_TM=60.018
PRIMER_LEFT_15_GC_PERCENT=30.435
PRIMER_RIGHT_15_GC_PERCENT=39.130
PRIMER_LEFT_15_SELF_ANY=8.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=2.00
PRIMER_RIGHT_15_SELF_END=2.00
PRIMER_LEFT_15_MISPRIMING_SCORE=11.00, L1MD1 3'-end of L1 repeat (subfamily L1MD1) - a consensus sequence
PRIMER_RIGHT_15_MISPRIMING_SCORE=12.00, MER34 a consensus
PRIMER_PAIR_15_MISPRIMING_SCORE=22.00, L1MD1 3'-end of L1 repeat (subfamily L1MD1) - a consensus sequence
PRIMER_LEFT_15_END_STABILITY=11.1000
PRIMER_RIGHT_15_END_STABILITY=6.4000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_15=707
PRIMER_PAIR_PENALTY_16=0.1308
PRIMER_LEFT_16_PENALTY=0.012045
PRIMER_RIGHT_16_PENALTY=0.118715
PRIMER_LEFT_16_SEQUENCE=GCTGCCACTAGTACTGAAAGCAT
PRIMER_RIGHT_16_SEQUENCE=GTGGACATTCTATGCACATTTCA
PRIMER_LEFT_16=1925,23
PRIMER_RIGHT_16=2249,23
PRIMER_LEFT_16_TM=59.988
PRIMER_RIGHT_16_TM=59.881
PRIMER_LEFT_16_GC_PERCENT=47.826
PRIMER_RIGHT_16_GC_PERCENT=39.130
PRIMER_LEFT_16_SELF_ANY=7.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=2.00
PRIMER_RIGHT_16_SELF_END=2.00
PRIMER_LEFT_16_MISPRIMING_SCORE=12.00, reverse MER7B Nonautonomous DNA transposon
PRIMER_RIGHT_16_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_16_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_16_END_STABILITY=8.1000
PRIMER_RIGHT_16_END_STABILITY=7.3000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_16=325
PRIMER_PAIR_PENALTY_17=0.1335
PRIMER_LEFT_17_PENALTY=0.012045
PRIMER_RIGHT_17_PENALTY=0.121486
PRIMER_LEFT_17_SEQUENCE=GCTGCCACTAGTACTGAAAGCAT
PRIMER_RIGHT_17_SEQUENCE=TTACCAAAACCAATATCTGTGGC
PRIMER_LEFT_17=1925,23
PRIMER_RIGHT_17=2319,23
PRIMER_LEFT_17_TM=59.988
PRIMER_RIGHT_17_TM=60.121
PRIMER_LEFT_17_GC_PERCENT=47.826
PRIMER_RIGHT_17_GC_PERCENT=39.130
PRIMER_LEFT_17_SELF_ANY=7.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=2.00
PRIMER_RIGHT_17_SELF_END=2.00
PRIMER_LEFT_17_MISPRIMING_SCORE=12.00, reverse MER7B Nonautonomous DNA transposon
PRIMER_RIGHT_17_MISPRIMING_SCORE=11.00, MER42a MER42 repetitive sequence: subfamily MER42a - a consensus
PRIMER_PAIR_17_MISPRIMING_SCORE=22.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_17_END_STABILITY=8.1000
PRIMER_RIGHT_17_END_STABILITY=9.4000
PRIMER_PAIR_17_COMPL_ANY=6.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_17=395
PRIMER_PAIR_PENALTY_18=0.1488
PRIMER_LEFT_18_PENALTY=0.100425
PRIMER_RIGHT_18_PENALTY=0.048411
PRIMER_LEFT_18_SEQUENCE=CTGAAGGCTTGATAACCTCTGAA
PRIMER_RIGHT_18_SEQUENCE=ATTGTTCCAAGGCACTTACTGAA
PRIMER_LEFT_18=2166,23
PRIMER_RIGHT_18=3031,23
PRIMER_LEFT_18_TM=59.900
PRIMER_RIGHT_18_TM=60.048
PRIMER_LEFT_18_GC_PERCENT=43.478
PRIMER_RIGHT_18_GC_PERCENT=39.130
PRIMER_LEFT_18_SELF_ANY=5.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_18_MISPRIMING_SCORE=12.00, MER6 Nonautonomous DNA transposon
PRIMER_PAIR_18_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_18_END_STABILITY=7.0000
PRIMER_RIGHT_18_END_STABILITY=7.0000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_18=866
PRIMER_PAIR_PENALTY_19=0.1488
PRIMER_LEFT_19_PENALTY=0.100425
PRIMER_RIGHT_19_PENALTY=0.048411
PRIMER_LEFT_19_SEQUENCE=CTGAAGGCTTGATAACCTCTGAA
PRIMER_RIGHT_19_SEQUENCE=TTGTTCCAAGGCACTTACTGAAT
PRIMER_LEFT_19=2166,23
PRIMER_RIGHT_19=3030,23
PRIMER_LEFT_19_TM=59.900
PRIMER_RIGHT_19_TM=60.048
PRIMER_LEFT_19_GC_PERCENT=43.478
PRIMER_RIGHT_19_GC_PERCENT=39.130
PRIMER_LEFT_19_SELF_ANY=5.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=2.00
PRIMER_LEFT_19_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_19_MISPRIMING_SCORE=12.00, MER6 Nonautonomous DNA transposon
PRIMER_PAIR_19_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_19_END_STABILITY=7.0000
PRIMER_RIGHT_19_END_STABILITY=6.9000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_19=865
=