PRIMER_SEQUENCE_ID=NM_002839
SEQUENCE=GCTAACTCAAGGGAGACGTCTGGTGAACACCCGTGGGATCTAAAGAACAAGCTCTGAAAGTGTTCCAGCTGAAATTTCAGATCGGACAGACTCGCTGCGGCTCCGGAGGCAGTGATTCCAAGCTGCTCGCGCACGCTGCTGCCAAGCTGCAGGATGGTGCACGTAGCCAGGCTGCTGCTGCTGCTCCTCACTTTCTTCCTCCGCACGGATGCTGAGACACCTCCAAGGTTTACACGAACACCCGTTGATCAGACAGGGGTCTCTGGCGGAGTTGCCTCTTTCATCTGCCAAGCTACGGGAGACCCAAGACCTAAAATTGTCTGGAACAAAAAAGGAAAGAAAGTCAGCAATCAGAGATTTGAGGTAATAGAGTTTGACGATGGGTCTGGATCAGTTCTCAGAATACAACCCTTACGGACTCCGAGGGATGAGGCCATTTATGAATGTGTGGCCTCAAATAATGTGGGAGAAATAAGTGTATCCACCAGACTCACAGTTTTGCGGGAAGATCAAATTCCCAGGGGCTTCCCTACCATTGACATGGGCCCACAGTTGAAGGTGGTTGAGCGTACTCGCACGGCCACCATGCTTTGTGCAGCCAGTGGTAATCCGGATCCAGAAATCACTTGGTTTAAAGATTTCTTACCTGTGGACACAAGCAACAACAATGGTCGTATTAAGCAGTTACGATCAGAATCTATTGGTGGTACACCAATAAGAGGAGCCCTTCAGATTGAGCAGAGTGAAGAGTCTGACCAAGGAAAATATGAGTGTGTTGCCACCAACAGCGCGGGCACTCGCTATTCCGCTCCTGCCAATTTATATGTCAGAGAGCTGCGAGAAGTTCGCCGTGTCCCACCAAGATTCTCTATCCCACCCACTAATCATGAAATCATGCCAGGCGGAAGCGTTAATATCACCTGTGTGGCCGTGGGGTCACCAATGCCTTATGTAAAGTGGATGTTGGGGGCAGAAGATCTGACACCTGAAGATGATATGCCAATAGGAAGAAATGTGCTAGAACTGAATGATGTAAGACAGTCAGCAAATTACACCTGTGTTGCTATGTCAACACTGGGTGTCATTGAAGCAATAGCACAGATCACTGTCAAAGCCTTACCCAAACCTCCAGGAACTCCTGTAGTGACCGAGAGCACAGCTACAAGCATCACACTGACGTGGGACTCTGGGAACCCTGAGCCTGTTTCTTATTACATAATTCAGCATAAACCTAAAAACTCTGAGGAACTTTACAAAGAAATTGATGGGGTGGCGACCACACGCTACAGTGTCGCTGGACTAAGTCCCTACTCGGATTATGAATTCAGGGTTGTTGCTGTCAATAACATTGGGCGGGGGCCTCCCAGCGAACCTGTGCTAACACAAACCTCAGAGCAAGCACCATCCAGTGCCCCGAGGGATGTCCAGGCACGAATGTTGAGTTCGACCACCATTTTGGTACAGTGGAAGGAACCTGAAGAGCCAAATGGACAGATCCAAGGATATAGAGTTTATTATACAATGGATCCCACTCAACATGTCAACAACTGGATGAAACACAATGTAGCTGACAGCCAAATCACTACTATTGGCAACTTAGTGCCCCAGAAAACATATTCTGTCAAAGTCCTGGCTTTTACCTCAATTGGAGATGGTCCCCTTTCAAGTGACATACAAGTCATCACTCAGACAGGAGTACCAGGGCAGCCACTAAACTTCAAAGCAGAACCTGAGTCTGAAACAAGTATTTTGCTCTCTTGGACACCTCCACGTTCAGATACCATTGCCAACTATGAACTGGTCTACAAAGATGGGGAGCATGGAGAGGAGCAACGAATTACCATTGAGCCAGGGACATCATATAGGCTGCAAGGACTGAAACCAAACAGCTTATACTATTTCCGTCTGGCTGCACGCTCCCCTCAAGGCCTGGGTGCTTCTACTGCAGAAATATCAGCTAGAACCATGCAGTCAAAGCCGTCAGCTCCTCCTCAAGACATTAGTTGCACCAGCCCAAGTTCCACTAGTATTTTGGTAAGTTGGCAACCTCCACCAGTGGAAAAACAGAATGGCATTATCACTGAATACTCCATCAAGTACACTGCAGTGGATGGGGAAGATGACAAGCCTCACGAGATTTTGGGAATTCCTTCGGACACTACCAAATACCTTTTGGAACAGCTGGAAAAATGGACTGAATACCGGATCACTGTGACAGCCCATACAGATGTCGGCCCTGGCCCTGAGAGCTTGTCCGTGTTGATTCGAACCAATGAAGATGTTCCTAGTGGTCCTCCTCGCAAAGTCGAGGTAGAGGCTGTCAACTCAACATCTGTTAAAGTCTCATGGCGCTCACCCGTGCCCAATAAACAGCATGGCCAGATAAGAGGATATCAGGTGCATTATGTGAGGATGGAAAATGGTGAGCCCAAGGGCCAGCCCATGCTGAAAGATGTCATGCTGGCTGATGCACAGTGGGAATTTGATGATACTACTGAACATGACATGATCATTTCTGGGCTCCAGCCTGAAACTTCCTACTCCCTCACCGTCACAGCCTACACAACCAAAGGAGATGGTGCTCGCAGCAAGCCCAAACTGGTGTCCACCACTGGGGCAGTTCCAGGGAAACCTCGGCTTGTGATTAACCACACTCAGATGAATACTGCTCTTATTCAGTGGCACCCTCCGGTGGACACATTTGGACCTCTTCAGGGCTACCGTCTAAAATTTGGCCGCAAGGATATGGAGCCACTTACTACTCTTGAGTTCTCTGAAAAAGAAGATCACTTTACAGCTACAGACATCCACAAGGGAGCATCATACGTCTTCAGGCTCTCAGCCAGAAACAAAGTGGGCTTTGGGGAGGAGATGGTGAAGGAGATTTCCATTCCAGAAGAAGTACCAACTGGATTCCCTCAAAACCTTCACTCAGAAGGCACCACTTCAACCTCCGTCCAGTTATCTTGGCAACCACCTGTCCTGGCAGAGAGAAATGGCATTATCACCAAGTATACCCTTCTTTATAGGGATATCAACATCCCCCTTCTCCCGATGGAGCAGCTTATTGTTCCAGCTGACACCACTATGACACTCACTGGCTTAAAACCAGATACCACATACGATGTAAAAGTACGTGCTCATACGAGCAAAGGGCCCGGGCCATATAGTCCCAGTGTCCAGTTCAGGACACTGCCTGTGGATCAAGTGTTTGCAAAAAATTTTCATGTCAAAGCAGTAATGAAGACTTCCGTGTTGCTGTCTTGGGAGATTCCAGAGAATTATAACTCCGCCATGCCTTTCAAAATTCTTTATGATGATGGGAAAATGGTAGAAGAAGTGGATGGCCGAGCCACACAGAAGTTAATTGTCAACCTGAAGCCTGAGAAATCATATTCATTTGTGCTGACAAATCGTGGAAACAGTGCTGGTGGGCTGCAGCACAGGGTCACGGCAAAGACTGCACCAGATGTATTACGTACCAAGCCTGCCTTCATTGGGAAGACCAACTTGGATGGCATGATTACTGTGCAACTGCCTGAAGTACCTGCAAATGAGAATATAAAAGGTTACTACATAATAATTGTGCCTTTGAAGAAATCTCGCGGGAAATTTATCAAGCCATGGGAGAGTCCAGATGAAATGGAATTAGATGAGCTGCTTAAGGAGATATCTAGGAAGCGCAGAAGCATCCGTTATGGGAGAGAAGTTGAATTAAAGCCATATATTGCCGCTCACTTTGATGTCCTTCCCACTGAGTTCACCCTGGGGGATGACAAGCATTATGGTGGATTTACAAACAAGCAACTCCAAAGTGGTCAAGAATATGTCTTCTTTGTGTTAGCAGTAATGGAACATGCAGAGTCTAAGATGTATGCAACCAGCCCTTACTCCGACCCCGTGGTGTCAATGGATCTGGATCCGCAGCCAATCACGGATGAAGAAGAAGGCTTGATCTGGGTTGTAGGTCCTGTCCTTGCAGTGGTCTTTATCATCTGCATTGTCATTGCTATTCTTCTTTATAAAAGGAAGAGGGCAGAGTCCGACTCTAGAAAAAGCAGCATACCGAACAATAAGGAGATCCCTTCACACCACCCAACAGACCCTGTAGAACTGAGGCGCCTTAACTTTCAAACACCGGGTATGGCTAGCCATCCTCCAATACCCATCTTGGAACTTGCAGACCACATTGAAAGATTGAAAGCAAATGACAACTTGAAGTTTTCCCAGGAATATGAGTCAATTGACCCTGGCCAGCAGTTCACTTGGGAACATTCAAACTTGGAAGTAAACAAACCAAAGAATAGATACGCGAATGTAATCGCATATGATCATTCCCGGGTTCTCCTATCAGCTATAGAAGGGATCCCAGGAAGTGACTATGTGAATGCCAACTACATAGATGGGTATAGGAAGCAAAATGCCTATATTGCAACACAGGGATCTCTCCCCGAAACATTTGGGGACTTTTGGAGAATGATATGGGAACAACGGAGTGCCACAGTTGTCATGATGACAAAACTAGAAGAAAGATCAAGGGTGAAGTGTGACCAGTATTGGCCTAGCAGAGGCACAGAAACCCACGGACTCGTTCAAGTAACGCTGCTTGATACTGTGGAGCTGGCCACATATTGTGTTCGAACATTTGCACTTTACAAGAATGGTTCAAGTGAGAAGAGAGAAGTGAGACAATTCCAGTTCACCGCCTGGCCTGATCATGGTGTTCCAGAACACCCTACACCTTTTCTAGCTTTCTTACGTAGAGTCAAAACCTGTAACCCTCCCGATGCTGGTCCGATGGTTGTGCACTGCAGTGCGGGAGTTGGCCGGACTGGTTGCTTCATCGTCATAGATGCCATGTTAGAAAGAATAAAGCATGAAAAAACTGTAGATATTTATGGCCATGTAACTTTAATGAGAGCCCAGAGGAACTATATGGTTCAAACAGAAGACCAATACATCTTTATCCATGATGCACTGTTAGAAGCAGTGACTTGTGGAAATACCGAAGTGCCAGCTAGAAACTTGTATGCCTACATTCAGAAGCTGACACAAATAGAAACGGGAGAGAATGTCACAGGAATGGAGCTCGAATTTAAGCGTCTAGCCAGCTCAAAAGCTCACACCTCAAGGTTTATCAGTGCCAATCTTCCATGTAATAAATTCAAAAATCGCCTTGTTAATATTATGCCATATGAATCCACAAGGGTATGCCTGCAGCCTATCCGTGGAGTAGAAGGATCTGATTACATCAATGCCAGTTTTATTGATGGATACAGACAACAGAAAGCCTACATCGCTACCCAGGGGCCCTTGGCAGAGACCACTGAAGACTTCTGGCGGATGCTCTGGGAACACAATTCCACCATAGTTGTGATGCTCACCAAGCTGCGTGAAATGGGCAGAGAGAAATGTCACCAATACTGGCCAGCAGAACGGTCTGCAAGATACCAGTACTTTGTTGTAGATCCCATGGCTGAGTACAACATGCCACAGTATATCCTAAGGGAATTCAAGGTCACAGATGCCAGGGACGGCCAGTCCCGAACAGTAAGGCAGTTCCAGTTCACTGACTGGCCAGAGCAAGGAGTGCCAAAGTCCGGAGAAGGATTTATTGACTTCATCGGCCAAGTCCATAAAACAAAAGAACAGTTTGGCCAAGATGGACCCATTTCAGTCCATTGCAGCGCGGGCGTTGGAAGAACTGGAGTCTTCATAACGCTAAGCATTGTTTTGGAAAGAATGAGATATGAAGGAGTTGTAGATATCTTCCAGACTGTCAAAATGTTAAGAACACAACGACCAGCTATGGTACAGACAGAGGATCAATATCAGTTTTCCTATCGTGCCGCACTAGAGTACCTGGGCAGCTTTGACCACTATGCAACGTAGAAACCCCTGACCCATTCTGGATTTTTACTACAGGCCCTTCAATATCCATGGAGTCTCTTCTGAGCCATACAGGGCACTTGAGAAGTCCTTCTTAACTTCTAGCTAACAACTACTTAGTGGGACTATTACACACAAAACAAATTAAAAACAAATTATTCCAGGTGGACCAAGAATTCTTTGACATCGCCCCTTCCCACCATACTGCTCATAATAACATTTTAGGGGCCAAGGGGAGGGAATGTTTAAAAAGAAAGTCCTTGATTTAGTTTTTTAGTATTGTAAAGATACTGCTGACCTGTGCTTCATTTCTAACTGTGTAAACTTTTTTTTAACAAAATGTATCATTCGATAAAGTGAATTTTAAAAAAGTT
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 73399, GC content failed 592, low tm 34284, high tm 21114, high any compl 8, high end compl 26, high repeat similarity 19, long poly-x seq 80, ok 17276
PRIMER_RIGHT_EXPLAIN=considered 73311, GC content failed 807, low tm 34360, high tm 20864, high any compl 3, high end compl 7, high repeat similarity 7, long poly-x seq 97, ok 17166
PRIMER_PAIR_EXPLAIN=considered 805, unacceptable product size 758, high end compl 2, ok 45
PRIMER_PAIR_PENALTY=0.0108
PRIMER_LEFT_PENALTY=0.007752
PRIMER_RIGHT_PENALTY=0.003045
PRIMER_LEFT_SEQUENCE=TCTCAGAATACAACCCTTACGGA
PRIMER_RIGHT_SEQUENCE=CTGACATATAAATTGGCAGGAGC
PRIMER_LEFT=395,23
PRIMER_RIGHT=829,23
PRIMER_LEFT_TM=60.008
PRIMER_RIGHT_TM=60.003
PRIMER_LEFT_GC_PERCENT=43.478
PRIMER_RIGHT_GC_PERCENT=43.478
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_MISPRIMING_SCORE=11.00, L1MA2 3'-end of L1 repeat (subfamily L1MA2) - a consensus sequence
PRIMER_RIGHT_MISPRIMING_SCORE=11.00, reverse L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_PAIR_MISPRIMING_SCORE=20.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_END_STABILITY=9.6000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=435
PRIMER_PAIR_PENALTY_1=0.0144
PRIMER_LEFT_1_PENALTY=0.000148
PRIMER_RIGHT_1_PENALTY=0.014220
PRIMER_LEFT_1_SEQUENCE=GACCACATTGAAAGATTGAAAGC
PRIMER_RIGHT_1_SEQUENCE=AGATTGGCACTGATAAACCTTGA
PRIMER_LEFT_1=4182,23
PRIMER_RIGHT_1=5149,23
PRIMER_LEFT_1_TM=60.000
PRIMER_RIGHT_1_TM=60.014
PRIMER_LEFT_1_GC_PERCENT=39.130
PRIMER_RIGHT_1_GC_PERCENT=39.130
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_MISPRIMING_SCORE=11.00, MER4C a consensus
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, PTR5 Human PTR5 mRNA for repetitive sequence
PRIMER_PAIR_1_MISPRIMING_SCORE=21.00, Tigger2 Autonomous DNA transposon
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=968
PRIMER_PAIR_PENALTY_2=0.0144
PRIMER_LEFT_2_PENALTY=0.006651
PRIMER_RIGHT_2_PENALTY=0.007752
PRIMER_LEFT_2_SEQUENCE=GACATCATATAGGCTGCAAGGAC
PRIMER_RIGHT_2_SEQUENCE=CTAGGAACATCTTCATTGGTTCG
PRIMER_LEFT_2=1853,23
PRIMER_RIGHT_2=2287,23
PRIMER_LEFT_2_TM=60.007
PRIMER_RIGHT_2_TM=60.008
PRIMER_LEFT_2_GC_PERCENT=47.826
PRIMER_RIGHT_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_2_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_2_MISPRIMING_SCORE=24.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=435
PRIMER_PAIR_PENALTY_3=0.0193
PRIMER_LEFT_3_PENALTY=0.006651
PRIMER_RIGHT_3_PENALTY=0.012680
PRIMER_LEFT_3_SEQUENCE=GACATCATATAGGCTGCAAGGAC
PRIMER_RIGHT_3_SEQUENCE=GAGGAGGACCACTAGGAACATCT
PRIMER_LEFT_3=1853,23
PRIMER_RIGHT_3=2298,23
PRIMER_LEFT_3_TM=60.007
PRIMER_RIGHT_3_TM=60.013
PRIMER_LEFT_3_GC_PERCENT=47.826
PRIMER_RIGHT_3_GC_PERCENT=52.174
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_3_MISPRIMING_SCORE=24.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_3_END_STABILITY=7.6000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=446
PRIMER_PAIR_PENALTY_4=0.0291
PRIMER_LEFT_4_PENALTY=0.026007
PRIMER_RIGHT_4_PENALTY=0.003045
PRIMER_LEFT_4_SEQUENCE=TCAGCTCCTCCTCAAGACATTAG
PRIMER_RIGHT_4_SEQUENCE=TCTTATCTGGCCATGCTGTTTAT
PRIMER_LEFT_4=1980,23
PRIMER_RIGHT_4=2387,23
PRIMER_LEFT_4_TM=60.026
PRIMER_RIGHT_4_TM=60.003
PRIMER_LEFT_4_GC_PERCENT=47.826
PRIMER_RIGHT_4_GC_PERCENT=39.130
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_MISPRIMING_SCORE=11.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_PAIR_4_MISPRIMING_SCORE=21.00, L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_LEFT_4_END_STABILITY=5.9000
PRIMER_RIGHT_4_END_STABILITY=6.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=408
PRIMER_PAIR_PENALTY_5=0.0327
PRIMER_LEFT_5_PENALTY=0.006651
PRIMER_RIGHT_5_PENALTY=0.026007
PRIMER_LEFT_5_SEQUENCE=GACATCATATAGGCTGCAAGGAC
PRIMER_RIGHT_5_SEQUENCE=CTAATGTCTTGAGGAGGAGCTGA
PRIMER_LEFT_5=1853,23
PRIMER_RIGHT_5=2002,23
PRIMER_LEFT_5_TM=60.007
PRIMER_RIGHT_5_TM=60.026
PRIMER_LEFT_5_GC_PERCENT=47.826
PRIMER_RIGHT_5_GC_PERCENT=47.826
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_5_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_5_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_5_END_STABILITY=7.6000
PRIMER_RIGHT_5_END_STABILITY=8.2000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_5=150
PRIMER_PAIR_PENALTY_6=0.0338
PRIMER_LEFT_6_PENALTY=0.026007
PRIMER_RIGHT_6_PENALTY=0.007752
PRIMER_LEFT_6_SEQUENCE=TCAGCTCCTCCTCAAGACATTAG
PRIMER_RIGHT_6_SEQUENCE=CTAGGAACATCTTCATTGGTTCG
PRIMER_LEFT_6=1980,23
PRIMER_RIGHT_6=2287,23
PRIMER_LEFT_6_TM=60.026
PRIMER_RIGHT_6_TM=60.008
PRIMER_LEFT_6_GC_PERCENT=47.826
PRIMER_RIGHT_6_GC_PERCENT=43.478
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=3.00
PRIMER_RIGHT_6_SELF_END=2.00
PRIMER_LEFT_6_MISPRIMING_SCORE=11.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_6_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_6_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_6_END_STABILITY=5.9000
PRIMER_RIGHT_6_END_STABILITY=8.4000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_6=308
PRIMER_PAIR_PENALTY_7=0.0345
PRIMER_LEFT_7_PENALTY=0.000148
PRIMER_RIGHT_7_PENALTY=0.034339
PRIMER_LEFT_7_SEQUENCE=GACCACATTGAAAGATTGAAAGC
PRIMER_RIGHT_7_SEQUENCE=TCCACAAGTCACTGCTTCTAACA
PRIMER_LEFT_7=4182,23
PRIMER_RIGHT_7=5000,23
PRIMER_LEFT_7_TM=60.000
PRIMER_RIGHT_7_TM=59.966
PRIMER_LEFT_7_GC_PERCENT=39.130
PRIMER_RIGHT_7_GC_PERCENT=43.478
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=5.00
PRIMER_LEFT_7_SELF_END=2.00
PRIMER_RIGHT_7_SELF_END=2.00
PRIMER_LEFT_7_MISPRIMING_SCORE=11.00, MER4C a consensus
PRIMER_RIGHT_7_MISPRIMING_SCORE=12.00, L1ME3a 3'-end of L1 repeat (subfamily L1ME3a) - a consensus sequence
PRIMER_PAIR_7_MISPRIMING_SCORE=22.00, MER4C a consensus
PRIMER_LEFT_7_END_STABILITY=8.5000
PRIMER_RIGHT_7_END_STABILITY=6.0000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_7=819
PRIMER_PAIR_PENALTY_8=0.0383
PRIMER_LEFT_8_PENALTY=0.038125
PRIMER_RIGHT_8_PENALTY=0.000148
PRIMER_LEFT_8_SEQUENCE=AAAAGCAGCATACCGAACAATAA
PRIMER_RIGHT_8_SEQUENCE=GCTTTCAATCTTTCAATGTGGTC
PRIMER_LEFT_8=4053,23
PRIMER_RIGHT_8=4204,23
PRIMER_LEFT_8_TM=60.038
PRIMER_RIGHT_8_TM=60.000
PRIMER_LEFT_8_GC_PERCENT=34.783
PRIMER_RIGHT_8_GC_PERCENT=39.130
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_8_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_8_END_STABILITY=6.2000
PRIMER_RIGHT_8_END_STABILITY=7.9000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_8=152
PRIMER_PAIR_PENALTY_9=0.0383
PRIMER_LEFT_9_PENALTY=0.038125
PRIMER_RIGHT_9_PENALTY=0.000148
PRIMER_LEFT_9_SEQUENCE=AAAAAGCAGCATACCGAACAATA
PRIMER_RIGHT_9_SEQUENCE=GCTTTCAATCTTTCAATGTGGTC
PRIMER_LEFT_9=4052,23
PRIMER_RIGHT_9=4204,23
PRIMER_LEFT_9_TM=60.038
PRIMER_RIGHT_9_TM=60.000
PRIMER_LEFT_9_GC_PERCENT=34.783
PRIMER_RIGHT_9_GC_PERCENT=39.130
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=2.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_9_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_9_END_STABILITY=6.2000
PRIMER_RIGHT_9_END_STABILITY=7.9000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_9=153
PRIMER_PAIR_PENALTY_10=0.0387
PRIMER_LEFT_10_PENALTY=0.026007
PRIMER_RIGHT_10_PENALTY=0.012680
PRIMER_LEFT_10_SEQUENCE=TCAGCTCCTCCTCAAGACATTAG
PRIMER_RIGHT_10_SEQUENCE=GAGGAGGACCACTAGGAACATCT
PRIMER_LEFT_10=1980,23
PRIMER_RIGHT_10=2298,23
PRIMER_LEFT_10_TM=60.026
PRIMER_RIGHT_10_TM=60.013
PRIMER_LEFT_10_GC_PERCENT=47.826
PRIMER_RIGHT_10_GC_PERCENT=52.174
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=3.00
PRIMER_RIGHT_10_SELF_END=2.00
PRIMER_LEFT_10_MISPRIMING_SCORE=11.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_10_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_10_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_10_END_STABILITY=5.9000
PRIMER_RIGHT_10_END_STABILITY=6.6000
PRIMER_PAIR_10_COMPL_ANY=8.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_10=319
PRIMER_PAIR_PENALTY_11=0.0414
PRIMER_LEFT_11_PENALTY=0.041227
PRIMER_RIGHT_11_PENALTY=0.000148
PRIMER_LEFT_11_SEQUENCE=GTCTAAGATGTATGCAACCAGCC
PRIMER_RIGHT_11_SEQUENCE=GCTTTCAATCTTTCAATGTGGTC
PRIMER_LEFT_11=3863,23
PRIMER_RIGHT_11=4204,23
PRIMER_LEFT_11_TM=60.041
PRIMER_RIGHT_11_TM=60.000
PRIMER_LEFT_11_GC_PERCENT=47.826
PRIMER_RIGHT_11_GC_PERCENT=39.130
PRIMER_LEFT_11_SELF_ANY=5.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=2.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_MISPRIMING_SCORE=12.00, reverse MER43 Homo sapiens clone DOE1-35 MER repetitive sequence - a consensus
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_11_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_11_END_STABILITY=9.7000
PRIMER_RIGHT_11_END_STABILITY=7.9000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_11=342
PRIMER_PAIR_PENALTY_12=0.0436
PRIMER_LEFT_12_PENALTY=0.017590
PRIMER_RIGHT_12_PENALTY=0.026007
PRIMER_LEFT_12_SEQUENCE=GAACCCTGAGCCTGTTTCTTATT
PRIMER_RIGHT_12_SEQUENCE=CTAATGTCTTGAGGAGGAGCTGA
PRIMER_LEFT_12=1190,23
PRIMER_RIGHT_12=2002,23
PRIMER_LEFT_12_TM=60.018
PRIMER_RIGHT_12_TM=60.026
PRIMER_LEFT_12_GC_PERCENT=43.478
PRIMER_RIGHT_12_GC_PERCENT=47.826
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_MISPRIMING_SCORE=11.00, MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_RIGHT_12_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_12_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_12_END_STABILITY=6.2000
PRIMER_RIGHT_12_END_STABILITY=8.2000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_12=813
PRIMER_PAIR_PENALTY_13=0.0479
PRIMER_LEFT_13_PENALTY=0.044904
PRIMER_RIGHT_13_PENALTY=0.003045
PRIMER_LEFT_13_SEQUENCE=TGTAGCTGACAGCCAAATCACTA
PRIMER_RIGHT_13_SEQUENCE=TCTTATCTGGCCATGCTGTTTAT
PRIMER_LEFT_13=1562,23
PRIMER_RIGHT_13=2387,23
PRIMER_LEFT_13_TM=59.955
PRIMER_RIGHT_13_TM=60.003
PRIMER_LEFT_13_GC_PERCENT=43.478
PRIMER_RIGHT_13_GC_PERCENT=39.130
PRIMER_LEFT_13_SELF_ANY=7.00
PRIMER_RIGHT_13_SELF_ANY=6.00
PRIMER_LEFT_13_SELF_END=2.00
PRIMER_RIGHT_13_SELF_END=2.00
PRIMER_LEFT_13_MISPRIMING_SCORE=12.00, MER4A a consensus
PRIMER_RIGHT_13_MISPRIMING_SCORE=12.00, L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_PAIR_13_MISPRIMING_SCORE=23.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_13_END_STABILITY=5.7000
PRIMER_RIGHT_13_END_STABILITY=6.2000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_13=826
PRIMER_PAIR_PENALTY_14=0.0486
PRIMER_LEFT_14_PENALTY=0.034339
PRIMER_RIGHT_14_PENALTY=0.014220
PRIMER_LEFT_14_SEQUENCE=TGTTAGAAGCAGTGACTTGTGGA
PRIMER_RIGHT_14_SEQUENCE=AGATTGGCACTGATAAACCTTGA
PRIMER_LEFT_14=4978,23
PRIMER_RIGHT_14=5149,23
PRIMER_LEFT_14_TM=59.966
PRIMER_RIGHT_14_TM=60.014
PRIMER_LEFT_14_GC_PERCENT=43.478
PRIMER_RIGHT_14_GC_PERCENT=39.130
PRIMER_LEFT_14_SELF_ANY=5.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=2.00
PRIMER_LEFT_14_MISPRIMING_SCORE=12.00, MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_RIGHT_14_MISPRIMING_SCORE=12.00, PTR5 Human PTR5 mRNA for repetitive sequence
PRIMER_PAIR_14_MISPRIMING_SCORE=21.00, MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_LEFT_14_END_STABILITY=7.9000
PRIMER_RIGHT_14_END_STABILITY=7.0000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_14=172
PRIMER_PAIR_PENALTY_15=0.0486
PRIMER_LEFT_15_PENALTY=0.048411
PRIMER_RIGHT_15_PENALTY=0.000148
PRIMER_LEFT_15_SEQUENCE=GTTAATTGTCAACCTGAAGCCTG
PRIMER_RIGHT_15_SEQUENCE=GCTTTCAATCTTTCAATGTGGTC
PRIMER_LEFT_15=3362,23
PRIMER_RIGHT_15=4204,23
PRIMER_LEFT_15_TM=60.048
PRIMER_RIGHT_15_TM=60.000
PRIMER_LEFT_15_GC_PERCENT=43.478
PRIMER_RIGHT_15_GC_PERCENT=39.130
PRIMER_LEFT_15_SELF_ANY=5.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=2.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_MISPRIMING_SCORE=12.00, MER21B a consensus
PRIMER_RIGHT_15_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_15_MISPRIMING_SCORE=22.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_15_END_STABILITY=9.7000
PRIMER_RIGHT_15_END_STABILITY=7.9000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_15=843
PRIMER_PAIR_PENALTY_16=0.0491
PRIMER_LEFT_16_PENALTY=0.007752
PRIMER_RIGHT_16_PENALTY=0.041315
PRIMER_LEFT_16_SEQUENCE=TCTCAGAATACAACCCTTACGGA
PRIMER_RIGHT_16_SEQUENCE=ATGATTTCATGATTAGTGGGTGG
PRIMER_LEFT_16=395,23
PRIMER_RIGHT_16=895,23
PRIMER_LEFT_16_TM=60.008
PRIMER_RIGHT_16_TM=59.959
PRIMER_LEFT_16_GC_PERCENT=43.478
PRIMER_RIGHT_16_GC_PERCENT=39.130
PRIMER_LEFT_16_SELF_ANY=5.00
PRIMER_RIGHT_16_SELF_ANY=6.00
PRIMER_LEFT_16_SELF_END=2.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_MISPRIMING_SCORE=11.00, L1MA2 3'-end of L1 repeat (subfamily L1MA2) - a consensus sequence
PRIMER_RIGHT_16_MISPRIMING_SCORE=12.00, reverse L1MC2 3'-end of L1 repeat (subfamily L1MC2) - a consensus sequence
PRIMER_PAIR_16_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_16_END_STABILITY=9.6000
PRIMER_RIGHT_16_END_STABILITY=9.4000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_16=501
PRIMER_PAIR_PENALTY_17=0.0499
PRIMER_LEFT_17_PENALTY=0.049761
PRIMER_RIGHT_17_PENALTY=0.000148
PRIMER_LEFT_17_SEQUENCE=CTCTAGAAAAAGCAGCATACCGA
PRIMER_RIGHT_17_SEQUENCE=GCTTTCAATCTTTCAATGTGGTC
PRIMER_LEFT_17=4046,23
PRIMER_RIGHT_17=4204,23
PRIMER_LEFT_17_TM=60.050
PRIMER_RIGHT_17_TM=60.000
PRIMER_LEFT_17_GC_PERCENT=43.478
PRIMER_RIGHT_17_GC_PERCENT=39.130
PRIMER_LEFT_17_SELF_ANY=6.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_17_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_17_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_17_END_STABILITY=9.6000
PRIMER_RIGHT_17_END_STABILITY=7.9000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_17=159
PRIMER_PAIR_PENALTY_18=0.0499
PRIMER_LEFT_18_PENALTY=0.049761
PRIMER_RIGHT_18_PENALTY=0.000148
PRIMER_LEFT_18_SEQUENCE=CCGACTCTAGAAAAAGCAGCATA
PRIMER_RIGHT_18_SEQUENCE=GCTTTCAATCTTTCAATGTGGTC
PRIMER_LEFT_18=4042,23
PRIMER_RIGHT_18=4204,23
PRIMER_LEFT_18_TM=60.050
PRIMER_RIGHT_18_TM=60.000
PRIMER_LEFT_18_GC_PERCENT=43.478
PRIMER_RIGHT_18_GC_PERCENT=39.130
PRIMER_LEFT_18_SELF_ANY=6.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=2.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_18_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_18_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_18_END_STABILITY=7.4000
PRIMER_RIGHT_18_END_STABILITY=7.9000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_18=163
PRIMER_PAIR_PENALTY_19=0.0527
PRIMER_LEFT_19_PENALTY=0.044904
PRIMER_RIGHT_19_PENALTY=0.007752
PRIMER_LEFT_19_SEQUENCE=TGTAGCTGACAGCCAAATCACTA
PRIMER_RIGHT_19_SEQUENCE=CTAGGAACATCTTCATTGGTTCG
PRIMER_LEFT_19=1562,23
PRIMER_RIGHT_19=2287,23
PRIMER_LEFT_19_TM=59.955
PRIMER_RIGHT_19_TM=60.008
PRIMER_LEFT_19_GC_PERCENT=43.478
PRIMER_RIGHT_19_GC_PERCENT=43.478
PRIMER_LEFT_19_SELF_ANY=7.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=2.00
PRIMER_RIGHT_19_SELF_END=2.00
PRIMER_LEFT_19_MISPRIMING_SCORE=12.00, MER4A a consensus
PRIMER_RIGHT_19_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_19_MISPRIMING_SCORE=24.00, MER4A a consensus
PRIMER_LEFT_19_END_STABILITY=5.7000
PRIMER_RIGHT_19_END_STABILITY=8.4000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_19=726
=