PRIMER_SEQUENCE_ID=NM_002841
SEQUENCE=ATGTTACTTCCTGTATGGAGGCATGGCCAGTTTCCAGCGCCGCGCTCTTCGTTCCTTCCCAGCCTGCGCCGGAGCCACAACTTTCAGGAGCATGGACTGAAGGCGCCCTCGCCCCAGCGCCCCTCTGAGATCCTTTGTGTTTTCCTCCGTTTCCTCCGGCCGTTTCTATTTTGGGGGGCTCTCCGCTCCCCCTGCCTCTCCCCTCCCCTTCCCCTCTCGCAAACATGCCTCCTTCCTTCCCGGGGCCCTGGAAGGAGCTGCCTGCCTGAAGCCCGGAGACGCCGCGCCGCGCTCAGCCCCGCCGCCGCCCGCCGGCTCTCGGGCTGTGCTGCGCTGCCGACTCAAGTTGGGGATCCTCGGCTGCTCGCCGCCGCCGCCCGCGGTCCCTGTCTGCCCCAGGCCCGGGGCATCGCCGCCGGCCGCCGACTCCGCGCCCTGCCCGATCGGCTCTCTCCTTTTTAAACGGAAAGCAGCCTTTCTCCGCCGAGAGGATCGTCCCCAGCGTGGCTCTGCGTTCCCGGTCACTTTTTGAGATTTTCCGGGGGGCGCTCGGCGGCTTCCCGGATTCCAAGGGGACTCGGGCCGCCGAGCGCGGGGGGCCCGTGGAGCGGGCGAGCCGGGGAAGCGCCCCGGCTTAGCGGAGGCTCGCACGGAGGCAAGAACTTATTCAACAAGTTTACCTCCCTGCTTTCCTCTTTTCGATGTGCGTTTTCGGACATGCGGAGGTTACTGGAACCGTGTTGGTGGATTTTGTTCCTGAAAATCACCAGTTCCGTGCTCCATTATGTCGTGTGCTTCCCCGCGTTGACAGAAGGCTACGTTGGGGCCCTGCACGAGAATAGACACGGCAGCGCAGTGCAGATCCGCAGGCGCAAGGCTTCAGGCGACCCGTACTGGGCCTACTCTGGTGCCTATGGTCCTGAGCACTGGGTCACGTCTAGTGTCAGCTGTGGGAGCCGTCACCAGTCTCCTATTGACATTTTAGACCAGTATGCGCGTGTTGGGGAAGAATACCAGGAACTGCAACTCGATGGCTTCGACAATGAGTCTTCTAACAAAACCTGGATGAAAAACACAGGGAAAACAGTCGCCATCCTTCTGAAAGACGACTATTTTGTCAGTGGAGCTGGTCTACCTGGCAGATTCAAAGCTGAGAAGGTGGAATTTCACTGGGGCCACAGCAATGGCTCAGCGGGCTCTGAACACAGCATCAATGGCAGGAGGTTTCCTGTTGAGATGCAGATTTTCTTTTACAATCCAGATGACTTTGACAGCTTTCAAACCGCAATTTCTGAGAACAGAATAATCGGAGCCATGGCCATATTTTTTCAAGTCAGTCCGAGGGACAATTCTGCACTGGATCCTATTATCCACGGGTTGAAGGGTGTCGTACATCATGAGAAGGAGACCTTTCTGGATCCTTTCGTCCTCCGGGACCTCCTGCCTGCATCCCTGGGCAGCTATTATCGGTACACAGGTTCCTTGACCACACCACCGTGTAGCGAAATAGTGGAGTGGATAGTCTTCCGGAGACCCGTCCCCATCTCTTACCATCAGCTTGAGGCTTTTTATTCCATCTTCACCACGGAGCAGCAAGACCATGTCAAGTCGGTGGAGTATCTGAGAAATAACTTTCGACCACAGCAGCGTCTGCATGACAGGGTGGTGTCCAAGTCCGCCGTCCGTGACTCCTGGAACCACGACATGACAGACTTCTTAGAAAACCCACTGGGGACAGAAGCCTCTAAAGTTTGCAGCTCTCCACCCATCCACATGAAGGTGCAGCCTCTGAACCAGACGGCACTGCAGGTGTCCTGGAGCCAGCCGGAGACTATCTACCACCCACCCATCATGAACTACATGATCTCCTACAGCTGGACCAAGAATGAGGACGAGAAGGAGAAGACGTTTACAAAGGACAGCGACAAAGACTTGAAAGCCACCATTAGCCATGTCTCACCCGATAGCCTTTACCTGTTCCGAGTCCAGGCCGTGTGTCGGAACGACATGCGCAGCGACTTTAGCCAGACGATGCTGTTTCAAGCTAATACCACTCGAATATTCCAAGGGACCAGAATAGTGAAAACAGGAGTGCCCACAGCGTCTCCTGCCTCTTCAGCCGACATGGCCCCCATCAGCTCGGGGTCTTCTACCTGGACGTCCTCTGGCATCCCATTCTCATTTGTTTCCATGGCAACTGGGATGGGCCCCTCCTCCAGTGGCAGCCAGGCCACAGTGGCCTCGGTGGTCACCAGCACGCTGCTCGCCGGCCTGGGGTTCGGCGGTGGTGGCATCTCCTCTTTCCCCAGCACTGTGTGGCCCACGCGCCTCCCGACGGCCGCCTCAGCCAGCAAGCAGGCGGCTAGGCCAGTCCTAGCCACCACAGAGGCCTTGGCTTCTCCAGGGCCCGATGGTGATTCGTCACCAACCAAGGACGGCGAGGGCACCGAGGAAGGAGAGAAGGATGAGAAAAGCGAGAGTGAGGATGGGGAGCGGGAGCACGAGGAGGATGGAGAGAAGGACTCCGAAAAGAAGGAGAAGAGTGGGGTGACCCACGCTGCCGAGGAGCGGAATCAGACGGAGCCCAGCCCCACACCCTCGTCTCCTAACAGGACTGCCGAGGGAGGGCATCAGACTATACCTGGGCATGAGCAGGATCACACTGCCGTCCCCACAGACCAGACGGGCGGAAGGAGGGATGCCGGCCCAGGCCTGGACCCCGACATGGTCACCTCCACCCAAGTGCCCCCCACCGCCACAGAGGAGCAGTATGCAGGGAGTGATCCCAAGAGGCCCGAAATGCCATCTAAAAAGCCTATGTCCCGCGGGGACCGATTTTCTGAAGACAGCAGATTTATCACTGTTAATCCAGCGGAAAAAAACACCTCTGGAATGATAAGCCGCCCTGCTCCAGGGAGGATGGAGTGGATCATCCCTCTGATTGTGGTATCAGCCTTGACCTTCGTGTGCCTCATCCTTCTCATTGCTGTGCTCGTTTACTGGAGAGGGTGTAACAAAATAAAGTCCAAGGGCTTTCCCAGACGTTTCCGTGAAGTGCCTTCTTCTGGGGAGAGAGGAGAGAAGGGGAGCAGAAAATGTTTTCAGACTGCTCATTTCTATGTGGAAGACAGCAGTTCACCTCGAGTGGTCCCTAATGAAAGTATTCCTATTATTCCTATTCCGGATGACATGGAAGCCATTCCTGTCAAACAGTTTGTCAAACACATCGGTGAGCTCTATTCTAATAACCAGCATGGGTTCTCTGAGGATTTTGAGGAAGTCCAGCGCTGTACTGCTGATATGAACATCACTGCAGAGCATTCCAATCATCCAGAAAACAAGCACAAAAACAGATACATCAACATTTTAGCATATGATCACAGTAGGGTGAAGTTAAGACCTTTACCAGGAAAAGACTCTAAGCACAGCGACTACATTAATGCAAACTATGTTGATGGTTACAACAAAGCAAAAGCCTACATTGCCACCCAAGGACCTTTGAAGTCTACATTTGAAGATTTCTGGAGGATGATTTGGGAACAAAACACTGGAATCATTGTGATGATTACGAACCTTGTGGAAAAAGGAAGACGAAAATGTGATCAGTATTGGCCAACAGAGAACAGTGAGGAATATGGAAACATTATTGTCACGCTGAAGAGCACAAAAATACATGCCTGCTACACTGTTCGTCGTTTTTCAATCAGAAATACAAAAGTGAAAAAGGGTCAGAAGGGAAATCCCAAGGGTCGTCAGAATGAAAGGGTAGTGATCCAGTATCACTATACACAGTGGCCTGACATGGGAGTTCCCGAGTATGCCCTTCCAGTACTGACTTTCGTGAGGAGATCCTCAGCAGCTCGGATGCCAGAAACGGGCCCTGTGTTGGTGCACTGCAGTGCTGGTGTGGGCAGAACAGGCACCTATATTGTAATAGACAGCATGCTGCAACAGATAAAAGACAAAAGCACAGTTAACGTCCTGGGATTCCTGAAGCATATCAGGACACAGCGTAACTACCTCGTCCAGACTGAGGAGCAGTACATTTTCATCCATGATGCCTTGTTGGAAGCCATTCTTGGAAAGGAGACTGAAGTATCTTCAAATCAGCTGCACAGCTATGTTAACAGCATCCTTATACCAGGAGTAGGAGGAAAGACACGACTGGAAAAGCAATTCAAGCTGGTCACACAGTGTAATGCAAAATATGTGGAATGTTTCAGTGCTCAGAAAGAGTGTAACAAAGAAAAGAACAGAAACTCTTCAGTTGTGCCATCTGAGCGTGCTCGAGTGGGTCTTGCACCATTGCCTGGAATGAAAGGAACAGATTACATTAATGCTTCTTATATCATGGGCTATTATAGGAGCAATGAATTTATTATAACTCAGCATCCTCTGCCACATACTACGAAAGATTTCTGGCGAATGATTTGGGATCATAACGCACAGATCATTGTCATGCTGCCAGACAACCAGAGCTTGGCAGAAGATGAGTTTGTGTACTGGCCAAGTCGAGAAGAATCCATGAACTGTGAGGCCTTTACCGTCACCCTTATCAGCAAAGACAGACTGTGCCTCTCTAATGAAGAACAAATTATCATCCATGACTTTATCCTTGAAGCTACACAGGATGACTATGTCTTAGAAGTTCGGCACTTTCAGTGTCCCAAATGGCCTAACCCAGATGCCCCCATAAGTAGTACCTTTGAACTTATCAACGTCATCAAGGAAGAGGCCTTAACAAGGGATGGTCCCACCATTGTTCATGATGAGTATGGAGCAGTTTCAGCAGGAATGTTATGTGCCCTTACCACCCTGTCCCAGCAACTGGAGAATGAAAATGCTGTGGATGTTTTCCAGGTTGCAAAAATGATCAATCTTATGAGGCCTGGAGTATTCACAGACATTGAACAATACCAGTTCATCTATAAAGCAAGGCTTAGCTTGGTCAGCACTAAAGAAAATGGAAATGGTCCCATGACAGTAGACAAAAATGGTGCTGTTCTTATTGCAGATGAATCAGACCCTGCTGAGAGCATGGAGTCCCTAGTGTGACTGGAATCCTGAAAGGGCACTTAATTTGTAAACTTCTGAAGACTGAGAACTTTTTTGAGGCCTTTTTTGCCAGACTCTAGGTTATACAATAACCCAGTTACTTTTTTACACTGATAAAAGTTTTGATATTTATTTTTTGCCATTTTATGTCTTAATGGTATCCTACTGAGCATTTGCACCTCTGTTCATTTCACACAGTGAAACGCAATTTTACCTAGTTTGCACTATATGATCAGTGTTACTGCCTATAATCTTATACAACAGCAAACCCTGATGTGACATTCCATGACGACATACATGCTACTTTTTTTTAGTTCAATACAGTGAAGGTCTTTGTTATGACAGTGAATATTGCTTTTATTATTATTATTGCTGAAGTGGTTGCATTCTACTAGCAGGCAATGCTGTACTTTTCTTCAGTCCTCCTCTCCTTTTTATTTTAGGCACTGTTCAATACTGTATGCCTTCTGTATTTTAATGGAGTGGATAGCATTGTTTTCTTTTACAGACTAGCAGGCTACTGGGACCTAAAAAGGTCTGTTAATGTCATGGCCTTGAAACAGTTCCATTTATGCTGGTTAAGAGATCCCTTAAGAAGTTAGAAGGCTTAAGAACTGCTTCATGTGAACATCCCTTATTAGTTACAAAGTTATATTCACAGTTTTTTAAAAATGTGTCAAAATAAAGGATAACTCTGTATTACAGCTTTCCCAGTAAAAAAAAAAAAAAAAA
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 66752, GC content failed 2728, low tm 27128, high tm 22424, high any compl 6, high end compl 36, high repeat similarity 49, long poly-x seq 193, ok 14188
PRIMER_RIGHT_EXPLAIN=considered 66522, GC content failed 2884, low tm 27453, high tm 21789, high end compl 7, high repeat similarity 18, long poly-x seq 225, ok 14146
PRIMER_PAIR_EXPLAIN=considered 1026, unacceptable product size 972, high any compl 1, high end compl 4, ok 49
PRIMER_PAIR_PENALTY=0.0292
PRIMER_LEFT_PENALTY=0.014945
PRIMER_RIGHT_PENALTY=0.014220
PRIMER_LEFT_SEQUENCE=CTAGCAGGCAATGCTGTACTTTT
PRIMER_RIGHT_SEQUENCE=AGAAAACAATGCTATCCACTCCA
PRIMER_LEFT=5446,23
PRIMER_RIGHT=5555,23
PRIMER_LEFT_TM=59.985
PRIMER_RIGHT_TM=60.014
PRIMER_LEFT_GC_PERCENT=43.478
PRIMER_RIGHT_GC_PERCENT=39.130
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_MISPRIMING_SCORE=12.00, reverse LTR5 LTR from human endogenous retrovirus 5' LTR, clone HERV-K18
PRIMER_RIGHT_MISPRIMING_SCORE=12.00, L1PA2 3'-end of L1 repeat (subfamily L1PA2) - a consensus sequence
PRIMER_PAIR_MISPRIMING_SCORE=20.00, L1PA2 3'-end of L1 repeat (subfamily L1PA2) - a consensus sequence
PRIMER_LEFT_END_STABILITY=7.3000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=110
PRIMER_PAIR_PENALTY_1=0.0348
PRIMER_LEFT_1_PENALTY=0.020535
PRIMER_RIGHT_1_PENALTY=0.014220
PRIMER_LEFT_1_SEQUENCE=CTTATTGCAGATGAATCAGACCC
PRIMER_RIGHT_1_SEQUENCE=AGAAAACAATGCTATCCACTCCA
PRIMER_LEFT_1=5004,23
PRIMER_RIGHT_1=5555,23
PRIMER_LEFT_1_TM=59.979
PRIMER_RIGHT_1_TM=60.014
PRIMER_LEFT_1_GC_PERCENT=43.478
PRIMER_RIGHT_1_GC_PERCENT=39.130
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, L1PA2 3'-end of L1 repeat (subfamily L1PA2) - a consensus sequence
PRIMER_PAIR_1_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_1_END_STABILITY=9.1000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=552
PRIMER_PAIR_PENALTY_2=0.0348
PRIMER_LEFT_2_PENALTY=0.020535
PRIMER_RIGHT_2_PENALTY=0.014220
PRIMER_LEFT_2_SEQUENCE=TTATTGCAGATGAATCAGACCCT
PRIMER_RIGHT_2_SEQUENCE=AGAAAACAATGCTATCCACTCCA
PRIMER_LEFT_2=5005,23
PRIMER_RIGHT_2=5555,23
PRIMER_LEFT_2_TM=59.979
PRIMER_RIGHT_2_TM=60.014
PRIMER_LEFT_2_GC_PERCENT=39.130
PRIMER_RIGHT_2_GC_PERCENT=39.130
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, LTR2 LTR from human endogenous retrovirus (4-14), 3' long terminal repeat
PRIMER_RIGHT_2_MISPRIMING_SCORE=12.00, L1PA2 3'-end of L1 repeat (subfamily L1PA2) - a consensus sequence
PRIMER_PAIR_2_MISPRIMING_SCORE=21.00, L1PA2 3'-end of L1 repeat (subfamily L1PA2) - a consensus sequence
PRIMER_LEFT_2_END_STABILITY=9.1000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=551
PRIMER_PAIR_PENALTY_3=0.0452
PRIMER_LEFT_3_PENALTY=0.020535
PRIMER_RIGHT_3_PENALTY=0.024653
PRIMER_LEFT_3_SEQUENCE=CTTATTGCAGATGAATCAGACCC
PRIMER_RIGHT_3_SEQUENCE=CCCAGTAGCCTGCTAGTCTGTAA
PRIMER_LEFT_3=5004,23
PRIMER_RIGHT_3=5579,23
PRIMER_LEFT_3_TM=59.979
PRIMER_RIGHT_3_TM=59.975
PRIMER_LEFT_3_GC_PERCENT=43.478
PRIMER_RIGHT_3_GC_PERCENT=52.174
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_3_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_3_END_STABILITY=9.1000
PRIMER_RIGHT_3_END_STABILITY=6.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=576
PRIMER_PAIR_PENALTY_4=0.0452
PRIMER_LEFT_4_PENALTY=0.020535
PRIMER_RIGHT_4_PENALTY=0.024653
PRIMER_LEFT_4_SEQUENCE=TTATTGCAGATGAATCAGACCCT
PRIMER_RIGHT_4_SEQUENCE=CCCAGTAGCCTGCTAGTCTGTAA
PRIMER_LEFT_4=5005,23
PRIMER_RIGHT_4=5579,23
PRIMER_LEFT_4_TM=59.979
PRIMER_RIGHT_4_TM=59.975
PRIMER_LEFT_4_GC_PERCENT=39.130
PRIMER_RIGHT_4_GC_PERCENT=52.174
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, LTR2 LTR from human endogenous retrovirus (4-14), 3' long terminal repeat
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_4_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_4_END_STABILITY=9.1000
PRIMER_RIGHT_4_END_STABILITY=6.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=575
PRIMER_PAIR_PENALTY_5=0.0554
PRIMER_LEFT_5_PENALTY=0.041227
PRIMER_RIGHT_5_PENALTY=0.014220
PRIMER_LEFT_5_SEQUENCE=GTATCCTACTGAGCATTTGCACC
PRIMER_RIGHT_5_SEQUENCE=AGAAAACAATGCTATCCACTCCA
PRIMER_LEFT_5=5212,23
PRIMER_RIGHT_5=5555,23
PRIMER_LEFT_5_TM=60.041
PRIMER_RIGHT_5_TM=60.014
PRIMER_LEFT_5_GC_PERCENT=47.826
PRIMER_RIGHT_5_GC_PERCENT=39.130
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=5.00
PRIMER_LEFT_5_SELF_END=2.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_5_MISPRIMING_SCORE=12.00, L1PA2 3'-end of L1 repeat (subfamily L1PA2) - a consensus sequence
PRIMER_PAIR_5_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_5_END_STABILITY=9.4000
PRIMER_RIGHT_5_END_STABILITY=8.2000
PRIMER_PAIR_5_COMPL_ANY=6.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_5=344
PRIMER_PAIR_PENALTY_6=0.0562
PRIMER_LEFT_6_PENALTY=0.053000
PRIMER_RIGHT_6_PENALTY=0.003218
PRIMER_LEFT_6_SEQUENCE=AGCAGAAAATGTTTTCAGACTGC
PRIMER_RIGHT_6_SEQUENCE=ATTTCTGATTGAAAAACGACGAA
PRIMER_LEFT_6=3087,23
PRIMER_RIGHT_6=3710,23
PRIMER_LEFT_6_TM=59.947
PRIMER_RIGHT_6_TM=59.997
PRIMER_LEFT_6_GC_PERCENT=39.130
PRIMER_RIGHT_6_GC_PERCENT=30.435
PRIMER_LEFT_6_SELF_ANY=8.00
PRIMER_RIGHT_6_SELF_ANY=5.00
PRIMER_LEFT_6_SELF_END=3.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_MISPRIMING_SCORE=12.00, MARINER1 Human mariner
PRIMER_RIGHT_6_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_6_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_6_END_STABILITY=7.9000
PRIMER_RIGHT_6_END_STABILITY=8.4000
PRIMER_PAIR_6_COMPL_ANY=6.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_6=624
PRIMER_PAIR_PENALTY_7=0.0594
PRIMER_LEFT_7_PENALTY=0.056142
PRIMER_RIGHT_7_PENALTY=0.003218
PRIMER_LEFT_7_SEQUENCE=GATGGTTACAACAAAGCAAAAGC
PRIMER_RIGHT_7_SEQUENCE=ATTTCTGATTGAAAAACGACGAA
PRIMER_LEFT_7=3453,23
PRIMER_RIGHT_7=3710,23
PRIMER_LEFT_7_TM=60.056
PRIMER_RIGHT_7_TM=59.997
PRIMER_LEFT_7_GC_PERCENT=39.130
PRIMER_RIGHT_7_GC_PERCENT=30.435
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=5.00
PRIMER_LEFT_7_SELF_END=2.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_7_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_7_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_7_END_STABILITY=8.5000
PRIMER_RIGHT_7_END_STABILITY=8.4000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_7=258
PRIMER_PAIR_PENALTY_8=0.0624
PRIMER_LEFT_8_PENALTY=0.003218
PRIMER_RIGHT_8_PENALTY=0.059210
PRIMER_LEFT_8_SEQUENCE=TTCGTCGTTTTTCAATCAGAAAT
PRIMER_RIGHT_8_SEQUENCE=TGAAAGTGCCGAACTTCTAAGAC
PRIMER_LEFT_8=3688,23
PRIMER_RIGHT_8=4660,23
PRIMER_LEFT_8_TM=59.997
PRIMER_RIGHT_8_TM=59.941
PRIMER_LEFT_8_GC_PERCENT=30.435
PRIMER_RIGHT_8_GC_PERCENT=43.478
PRIMER_LEFT_8_SELF_ANY=5.00
PRIMER_RIGHT_8_SELF_ANY=7.00
PRIMER_LEFT_8_SELF_END=3.00
PRIMER_RIGHT_8_SELF_END=3.00
PRIMER_LEFT_8_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, reverse THE1BR THE-1B Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_PAIR_8_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_8_END_STABILITY=6.9000
PRIMER_RIGHT_8_END_STABILITY=6.4000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_8=973
PRIMER_PAIR_PENALTY_9=0.0659
PRIMER_LEFT_9_PENALTY=0.041227
PRIMER_RIGHT_9_PENALTY=0.024653
PRIMER_LEFT_9_SEQUENCE=GTATCCTACTGAGCATTTGCACC
PRIMER_RIGHT_9_SEQUENCE=CCCAGTAGCCTGCTAGTCTGTAA
PRIMER_LEFT_9=5212,23
PRIMER_RIGHT_9=5579,23
PRIMER_LEFT_9_TM=60.041
PRIMER_RIGHT_9_TM=59.975
PRIMER_LEFT_9_GC_PERCENT=47.826
PRIMER_RIGHT_9_GC_PERCENT=52.174
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=2.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_9_MISPRIMING_SCORE=24.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_9_END_STABILITY=9.4000
PRIMER_RIGHT_9_END_STABILITY=6.0000
PRIMER_PAIR_9_COMPL_ANY=6.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_9=368
PRIMER_PAIR_PENALTY_10=0.0718
PRIMER_LEFT_10_PENALTY=0.034654
PRIMER_RIGHT_10_PENALTY=0.037129
PRIMER_LEFT_10_SEQUENCE=TGTAGCGAAATAGTGGAGTGGAT
PRIMER_RIGHT_10_SEQUENCE=ACTCGGAACAGGTAAAGGCTATC
PRIMER_LEFT_10=1497,23
PRIMER_RIGHT_10=1984,23
PRIMER_LEFT_10_TM=60.035
PRIMER_RIGHT_10_TM=60.037
PRIMER_LEFT_10_GC_PERCENT=43.478
PRIMER_RIGHT_10_GC_PERCENT=47.826
PRIMER_LEFT_10_SELF_ANY=2.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=2.00
PRIMER_RIGHT_10_SELF_END=2.00
PRIMER_LEFT_10_MISPRIMING_SCORE=12.00, reverse MER4A a consensus
PRIMER_RIGHT_10_MISPRIMING_SCORE=10.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_10_MISPRIMING_SCORE=21.00, reverse MER4A a consensus
PRIMER_LEFT_10_END_STABILITY=8.1000
PRIMER_RIGHT_10_END_STABILITY=5.6000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_10=488
PRIMER_PAIR_PENALTY_11=0.0718
PRIMER_LEFT_11_PENALTY=0.034654
PRIMER_RIGHT_11_PENALTY=0.037129
PRIMER_LEFT_11_SEQUENCE=TGTAGCGAAATAGTGGAGTGGAT
PRIMER_RIGHT_11_SEQUENCE=GACTCGGAACAGGTAAAGGCTAT
PRIMER_LEFT_11=1497,23
PRIMER_RIGHT_11=1985,23
PRIMER_LEFT_11_TM=60.035
PRIMER_RIGHT_11_TM=60.037
PRIMER_LEFT_11_GC_PERCENT=43.478
PRIMER_RIGHT_11_GC_PERCENT=47.826
PRIMER_LEFT_11_SELF_ANY=2.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=2.00
PRIMER_RIGHT_11_SELF_END=3.00
PRIMER_LEFT_11_MISPRIMING_SCORE=12.00, reverse MER4A a consensus
PRIMER_RIGHT_11_MISPRIMING_SCORE=11.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_11_MISPRIMING_SCORE=20.00, MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_LEFT_11_END_STABILITY=8.1000
PRIMER_RIGHT_11_END_STABILITY=7.1000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_11=489
PRIMER_PAIR_PENALTY_12=0.0778
PRIMER_LEFT_12_PENALTY=0.063624
PRIMER_RIGHT_12_PENALTY=0.014220
PRIMER_LEFT_12_SEQUENCE=CTAAAGAAAATGGAAATGGTCCC
PRIMER_RIGHT_12_SEQUENCE=AGAAAACAATGCTATCCACTCCA
PRIMER_LEFT_12=4954,23
PRIMER_RIGHT_12=5555,23
PRIMER_LEFT_12_TM=60.064
PRIMER_RIGHT_12_TM=60.014
PRIMER_LEFT_12_GC_PERCENT=39.130
PRIMER_RIGHT_12_GC_PERCENT=39.130
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=5.00
PRIMER_LEFT_12_SELF_END=2.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_12_MISPRIMING_SCORE=12.00, L1PA2 3'-end of L1 repeat (subfamily L1PA2) - a consensus sequence
PRIMER_PAIR_12_MISPRIMING_SCORE=21.00, L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence
PRIMER_LEFT_12_END_STABILITY=9.1000
PRIMER_RIGHT_12_END_STABILITY=8.2000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_12=602
PRIMER_PAIR_PENALTY_13=0.0808
PRIMER_LEFT_13_PENALTY=0.077579
PRIMER_RIGHT_13_PENALTY=0.003218
PRIMER_LEFT_13_SEQUENCE=ACCCAAGGACCTTTGAAGTCTAC
PRIMER_RIGHT_13_SEQUENCE=ATTTCTGATTGAAAAACGACGAA
PRIMER_LEFT_13=3486,23
PRIMER_RIGHT_13=3710,23
PRIMER_LEFT_13_TM=59.922
PRIMER_RIGHT_13_TM=59.997
PRIMER_LEFT_13_GC_PERCENT=47.826
PRIMER_RIGHT_13_GC_PERCENT=30.435
PRIMER_LEFT_13_SELF_ANY=7.00
PRIMER_RIGHT_13_SELF_ANY=5.00
PRIMER_LEFT_13_SELF_END=3.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_MISPRIMING_SCORE=11.00, MSTAR MSTa-Mammalian LTR internal retrotransposon sequence - a consensus
PRIMER_RIGHT_13_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_13_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_13_END_STABILITY=5.4000
PRIMER_RIGHT_13_END_STABILITY=8.4000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_13=225
PRIMER_PAIR_PENALTY_14=0.0883
PRIMER_LEFT_14_PENALTY=0.063624
PRIMER_RIGHT_14_PENALTY=0.024653
PRIMER_LEFT_14_SEQUENCE=CTAAAGAAAATGGAAATGGTCCC
PRIMER_RIGHT_14_SEQUENCE=CCCAGTAGCCTGCTAGTCTGTAA
PRIMER_LEFT_14=4954,23
PRIMER_RIGHT_14=5579,23
PRIMER_LEFT_14_TM=60.064
PRIMER_RIGHT_14_TM=59.975
PRIMER_LEFT_14_GC_PERCENT=39.130
PRIMER_RIGHT_14_GC_PERCENT=52.174
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=2.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_14_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_14_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_14_END_STABILITY=9.1000
PRIMER_RIGHT_14_END_STABILITY=6.0000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_14=626
PRIMER_PAIR_PENALTY_15=0.0905
PRIMER_LEFT_15_PENALTY=0.020535
PRIMER_RIGHT_15_PENALTY=0.069993
PRIMER_LEFT_15_SEQUENCE=TTATTGCAGATGAATCAGACCCT
PRIMER_RIGHT_15_SEQUENCE=TGCAAACTAGGTAAAATTGCGTT
PRIMER_LEFT_15=5005,23
PRIMER_RIGHT_15=5278,23
PRIMER_LEFT_15_TM=59.979
PRIMER_RIGHT_15_TM=60.070
PRIMER_LEFT_15_GC_PERCENT=39.130
PRIMER_RIGHT_15_GC_PERCENT=34.783
PRIMER_LEFT_15_SELF_ANY=5.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_MISPRIMING_SCORE=12.00, LTR2 LTR from human endogenous retrovirus (4-14), 3' long terminal repeat
PRIMER_RIGHT_15_MISPRIMING_SCORE=12.00, MER42a MER42 repetitive sequence: subfamily MER42a - a consensus
PRIMER_PAIR_15_MISPRIMING_SCORE=21.00, MER42a MER42 repetitive sequence: subfamily MER42a - a consensus
PRIMER_LEFT_15_END_STABILITY=9.1000
PRIMER_RIGHT_15_END_STABILITY=9.9000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_15=274
PRIMER_PAIR_PENALTY_16=0.0905
PRIMER_LEFT_16_PENALTY=0.020535
PRIMER_RIGHT_16_PENALTY=0.069993
PRIMER_LEFT_16_SEQUENCE=CTTATTGCAGATGAATCAGACCC
PRIMER_RIGHT_16_SEQUENCE=TGCAAACTAGGTAAAATTGCGTT
PRIMER_LEFT_16=5004,23
PRIMER_RIGHT_16=5278,23
PRIMER_LEFT_16_TM=59.979
PRIMER_RIGHT_16_TM=60.070
PRIMER_LEFT_16_GC_PERCENT=43.478
PRIMER_RIGHT_16_GC_PERCENT=34.783
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_16_MISPRIMING_SCORE=12.00, MER42a MER42 repetitive sequence: subfamily MER42a - a consensus
PRIMER_PAIR_16_MISPRIMING_SCORE=22.00, MER42a MER42 repetitive sequence: subfamily MER42a - a consensus
PRIMER_LEFT_16_END_STABILITY=9.1000
PRIMER_RIGHT_16_END_STABILITY=9.9000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_16=275
PRIMER_PAIR_PENALTY_17=0.0911
PRIMER_LEFT_17_PENALTY=0.087907
PRIMER_RIGHT_17_PENALTY=0.003218
PRIMER_LEFT_17_SEQUENCE=TTGTGATGATTACGAACCTTGTG
PRIMER_RIGHT_17_SEQUENCE=ATTTCTGATTGAAAAACGACGAA
PRIMER_LEFT_17=3556,23
PRIMER_RIGHT_17=3710,23
PRIMER_LEFT_17_TM=59.912
PRIMER_RIGHT_17_TM=59.997
PRIMER_LEFT_17_GC_PERCENT=39.130
PRIMER_RIGHT_17_GC_PERCENT=30.435
PRIMER_LEFT_17_SELF_ANY=5.00
PRIMER_RIGHT_17_SELF_ANY=5.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_MISPRIMING_SCORE=12.00, reverse LTR12 LTR from human ERV9 endogenous retroviral sequence (HRES-1/1)
PRIMER_RIGHT_17_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_17_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_17_END_STABILITY=7.0000
PRIMER_RIGHT_17_END_STABILITY=8.4000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_17=155
PRIMER_PAIR_PENALTY_18=0.0923
PRIMER_LEFT_18_PENALTY=0.033117
PRIMER_RIGHT_18_PENALTY=0.059210
PRIMER_LEFT_18_SEQUENCE=TGGGCTATTATAGGAGCAATGAA
PRIMER_RIGHT_18_SEQUENCE=TGAAAGTGCCGAACTTCTAAGAC
PRIMER_LEFT_18=4351,23
PRIMER_RIGHT_18=4660,23
PRIMER_LEFT_18_TM=59.967
PRIMER_RIGHT_18_TM=59.941
PRIMER_LEFT_18_GC_PERCENT=39.130
PRIMER_RIGHT_18_GC_PERCENT=43.478
PRIMER_LEFT_18_SELF_ANY=7.00
PRIMER_RIGHT_18_SELF_ANY=7.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=3.00
PRIMER_LEFT_18_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_18_MISPRIMING_SCORE=12.00, reverse THE1BR THE-1B Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_PAIR_18_MISPRIMING_SCORE=23.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_18_END_STABILITY=6.9000
PRIMER_RIGHT_18_END_STABILITY=6.4000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_18=310
PRIMER_PAIR_PENALTY_19=0.0967
PRIMER_LEFT_19_PENALTY=0.033117
PRIMER_RIGHT_19_PENALTY=0.063624
PRIMER_LEFT_19_SEQUENCE=TGGGCTATTATAGGAGCAATGAA
PRIMER_RIGHT_19_SEQUENCE=GGGACCATTTCCATTTTCTTTAG
PRIMER_LEFT_19=4351,23
PRIMER_RIGHT_19=4976,23
PRIMER_LEFT_19_TM=59.967
PRIMER_RIGHT_19_TM=60.064
PRIMER_LEFT_19_GC_PERCENT=39.130
PRIMER_RIGHT_19_GC_PERCENT=39.130
PRIMER_LEFT_19_SELF_ANY=7.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=2.00
PRIMER_LEFT_19_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_19_MISPRIMING_SCORE=11.00, L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence
PRIMER_PAIR_19_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_19_END_STABILITY=6.9000
PRIMER_RIGHT_19_END_STABILITY=6.3000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_19=626
=