PRIMER_SEQUENCE_ID=NM_002842
SEQUENCE=CTAGGCCTGGGACTCCTGGGTCCCCGGCAGTGTCTGGAGGCATGGCTGGGGCTGGCGGGGGCCTCGGGGTCTGGGGGAACCTGGTGCTGCTGGGCCTGTGCAGCTGGACAGGGGCCAGGGCGCCTGCCCCCAACCCAGGGAGGAACCTGACAGTGGAGACTCAGACCACCAGCTCCATCTCCCTGAGCTGGGAGGTCCCCGATGGCCTAGACTCACAGAACTCCAACTACTGGGTTCAGTGTACTGGAGACGGCGGCACAACAGAGACTCGAAACACAACAGCCACCAACGTCACCGTGGATGGCCTTGGACCCGGGTCATTGTATACGTGTTCTGTGTGGGTGGAGAAAGACGGAGTAAATAGCTCTGTGGGGACTGTCACTACTGCCACAGCTCCCAACCCAGTGAGGAACCTGAGAGTGGAGGCTCAGACCAACAGCTCCATCGCCCTGACCTGGGAGGTCCCCGACGGCCCAGACCCACAGAACTCCACCTACGGGGTTGAGTACACTGGAGATGGTGGCAGAGCAGGGACTCGAAGCACAGCACACACTAACATCACCGTGGATGGACTTGAACCCGGGTGTTTGTATGCGTTTTCCATGTGGGTGGGAAAGAATGGAATCAACAGCTCCCGGGAGACTCGAAATGCCACCACAGCTCACAACCCAGTGAGGAAACCTGAGAGTGGAGGCTCAGACCACCAGCTCCATCTCCCTGAGCTGGGAGGTCCCCGATGGCACAGACCCACAGAACTCGACCTACTGCGTACGAGTGCACTGGAGATGGTGGCAGAACAGAGACTCGAAACACAACAGACACCAGAGTCACCAGTGGATGGCCTTGGACCCGGGTCATTGTATACGTGTTCTGTGTGGGTGGAGAAAGACGGAGTAAATAGCTCCTCGTGGAGATTGGTAACTAGTACCACAGCTCCCAACCCAGTGAGAAACCTGACAGTGGAGGCTCAGACCAACAGCTCCATCGCCCTGACCTGGGAGGTCCCCGATGGCCCAGACCCACAGAACTCCACCTACGGGGTTGAGTACACTGGAGATGGTGGCAGAGCAGGGACTCGAAGCACAGCACACACCAACATCACCGTGGATAGACTTGAACCCGGGTGTTTGTATGTGTTTTCCGTGTGGGTGGGGAAGAATGGAATCAACAGCTCCCGGGAGACTCGAAATGCCACCACAGCCCCCAACCCAGTGAGAAACCTCCATATGGAGACTCAGACCAACAGCTCCATCGCCCTATGCTGGGAAGTCCCCGATGGCCCATACCCTCAGGACTACACCTACTGGGTAGGGTACACTGGAGACGGTGGTGGCACAGAGACCCGAAACACAACAAATACCAGTGTGACAGCTGAGAGACTTGAGCCCGGAACCTTGTACACATTCTCTGTATGGGCAGAAAAAAATGGAGCACGTGGCTCCAGGCAGAATGTCAGCATCTCCACAGTCCCCAACGCAGTGACAAGCCTCAGCAAGCAGGACTGGACCAACAGCACCATTGCTTTGCGCTGGACAGCTCCCCAGGGCCCAGGCCAGTCTTCCTACAGCTACTGGGTCTCATGGGTCAGGGAAGGCATGACTGACCCCAGGACCCAAAGCACCTCAGGTACTGACATCACCCTAAAGGAACTGGAAGCTGGCAGCCTGTACCACCTCACCGTCTGGGCCGAGAGGAATGAGGTCAGAGGCTATAACAGCACCCTCACTGCAGCCACTGCTCCCAATGAGGTCACAGATCTCCAGAATGAAACTCAGACTAAGAACTCAGTCATGCTGTGGTGGAAGGCCCCTGGAGACCCCCACTCTCAGTTGTACGTATACTGGGTCCAGTGGGCCAGCAAGGGACATCCCCGGAGGGGGCAAGATCCCCAAGCGAATTGGGTCAACCAGACCAGCAGGACCAATGAGACGTGGTACAAAGTGGAGGCCCTGGAACCCGGGACGTTGTACAATTTCACCGTGTGGGCAGAGAGGAATGACGTAGCCAGTTCCACGCAGAGCCTCTGTGCGTCCACATACCCAGACACAGTCACCATCACTTCCTGTGTCAGCACCTCAGCGGGCTATGGAGTCAACTTGATCTGGTCCTGCCCCCAGGGAGGCTACGAGGCCTTTGAGTTGGAGGTGGGAGGACAGCGGGGCTCCCAGGACAGATCTTCATGTGGGGAGGCTGTGTCTGTGTTGGGTCTCGGGCCGGCTCGGTCCTACCCAGCCACCATCACGACCATCTGGGACGGAATGAAGGTCGTGTCTCACTCTGTGGTCTGCCACACCGAGAGTGCAGGGGTCATTGCCGGAGCCTTTGTGGGCATCCTCCTGTTTCTCATCCTCGTGGGCCTGCTGATTTTCTTCCTGAAGAGGAGGAATAAGAAGAAGCAGCAGAAACCAGAACTCAGGGATCTGGTCTTTAGCTCCCCAGGGGACATCCCAGCTGAAGACTTCGCTGACCACGTCAGGAAGAATGAGAGGGACAGCAACTGTGGTTTTGCAGACGAGTACCAGCAACTCTCCCTGGTGGGCCACAGCCAGTCTCAGATGGTGGCTTCGGCTTCAGAGAACAACGCCAAGAACCGCTACAGAAATGTGCTGCCCTATGACTGGTCCCGGGTGCCCCTGAAGCCCATCCATGAGGAGCCAGGCTCTGACTACATCAATGCCAGCTTCATGCCCGGTCTCTGGAGCCCCCAGGAGTTCATTGCAACCCAGGGTCCCCTGCCACAGACAGTGGGTGACTTCTGGCGCCTGGTGTGGGAACAGCAGAGCCACACCCTGGTCATGCTGACCAACTGCATGGAGGCCGGCCGGGTGAAGTGTGAGCATTACTGGCCTCTGGACTCGCAGCCCTGCACCCATGGGCACCTGCGGGTAACCCTGGTAGGTGAGGAAGTGATGGAGAACTGGACGGTGCGGGAACTGCTGCTCCTCCAGGTGGAGGAGCAGAAGACACTGTCTGTGCGCCAATTCCACTACCAGGCCTGGCCGGATCACGGCGTTCCCTCCTCCCCAGACACCTTGCTGGCTTTCTGGAGGATGCTTCGGCAGTGGCTGGATCAGACCATGGAGGGAGGCCCACCCATTGTGCACTGCAGTGCTGGCGTGGGTCGCACAGGAACCCTCATTGCCCTGGACGTCCTGCTCCGGCAGCTGCAGTCCGAGGGTCTCCTTGGGCCCTTCAGCTTTGTAAGGAAGATGAGAGAGAGTCGGCCGTTGATGGTGCAGACTGAGGCTCAGTACGTATTCCTGCATCAGTGCATCTGCGGTTCCTCCAACAGTCAGCCCAGGCCCCAGCCGAGAAGGAAGTCCCGTATGAGGATGTCGAAAACCTCATCTACGAGAACGTGGCCGCCATCCAGGCCCACAAGTTGGAGGTCTAAGTGACGAGGGGGCTGGGTCGGCAGCCCAGGCATCCTCAAGCTCTGGACACCCACTTGAGCCCAGATTCCTGGAAGAGCAGAGGGCTGGGCTCCCAGACTCCTGGGTGCTGTGGGAGGAGGGGGCTGGTATCCCAAACTCTGGTTTCCCCAGGAGAGAGTGGTCTGGTGGGCTTCAGATGAGTCCTATGGGAGCTGGGGATCTGGATTCCTGGTTCCCTGAAGGAGGAGAGGGATGATAGCTTGGATTCCCTAGGTCTTTCCAGGATGCAGAAAGAAACAGGCTGGGGCCTGGATTCTGAGGCAGGAAGGAATTTGGGTCTGGAGTTCTGGCTACTTGAGGACCAAAGGCAGGAAGGATCCTGCCTTGATTTTACTTCAGAAACCAAATCAGTCTTCTATAATCTGGGGTCGGAGGGAGTCCCTGTGCCCAAGGTCTCTCTGCACCCCACCATCCACATGTATTTTTCCTTCTATCCCATAATTTATTAAATCACTGTTCTCCCCAG
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 45436, GC content failed 507, low tm 12566, high tm 22937, high any compl 22, high end compl 45, high repeat similarity 57, long poly-x seq 22, ok 9280
PRIMER_RIGHT_EXPLAIN=considered 45436, GC content failed 361, low tm 12971, high tm 22648, high any compl 4, high end compl 3, high repeat similarity 11, long poly-x seq 22, ok 9416
PRIMER_PAIR_EXPLAIN=considered 920, unacceptable product size 840, high any compl 5, high end compl 4, ok 71
PRIMER_PAIR_PENALTY=0.1589
PRIMER_LEFT_PENALTY=0.016975
PRIMER_RIGHT_PENALTY=0.141958
PRIMER_LEFT_SEQUENCE=AGGCCAGTCTTCCTACAGCTACT
PRIMER_RIGHT_SEQUENCE=GCTAAAGACCAGATCCCTGAGTT
PRIMER_LEFT=1549,23
PRIMER_RIGHT=2431,23
PRIMER_LEFT_TM=59.983
PRIMER_RIGHT_TM=60.142
PRIMER_LEFT_GC_PERCENT=52.174
PRIMER_RIGHT_GC_PERCENT=47.826
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_MISPRIMING_SCORE=12.00, reverse L1MD1 3'-end of L1 repeat (subfamily L1MD1) - a consensus sequence
PRIMER_PAIR_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_END_STABILITY=5.4000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=883
PRIMER_PAIR_PENALTY_1=0.1613
PRIMER_LEFT_1_PENALTY=0.016975
PRIMER_RIGHT_1_PENALTY=0.144318
PRIMER_LEFT_1_SEQUENCE=AGGCCAGTCTTCCTACAGCTACT
PRIMER_RIGHT_1_SEQUENCE=CCAGATCAAGTTGACTCCATAGC
PRIMER_LEFT_1=1549,23
PRIMER_RIGHT_1=2104,23
PRIMER_LEFT_1_TM=59.983
PRIMER_RIGHT_1_TM=60.144
PRIMER_LEFT_1_GC_PERCENT=52.174
PRIMER_RIGHT_1_GC_PERCENT=47.826
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_1_MISPRIMING_SCORE=24.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_1_END_STABILITY=5.4000
PRIMER_RIGHT_1_END_STABILITY=7.1000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=556
PRIMER_PAIR_PENALTY_2=0.1915
PRIMER_LEFT_2_PENALTY=0.049500
PRIMER_RIGHT_2_PENALTY=0.141958
PRIMER_LEFT_2_SEQUENCE=CTTCCTACAGCTACTGGGTCTCA
PRIMER_RIGHT_2_SEQUENCE=GCTAAAGACCAGATCCCTGAGTT
PRIMER_LEFT_2=1557,23
PRIMER_RIGHT_2=2431,23
PRIMER_LEFT_2_TM=59.950
PRIMER_RIGHT_2_TM=60.142
PRIMER_LEFT_2_GC_PERCENT=52.174
PRIMER_RIGHT_2_GC_PERCENT=47.826
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, MER43 Homo sapiens clone DOE1-35 MER repetitive sequence - a consensus
PRIMER_RIGHT_2_MISPRIMING_SCORE=12.00, reverse L1MD1 3'-end of L1 repeat (subfamily L1MD1) - a consensus sequence
PRIMER_PAIR_2_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=875
PRIMER_PAIR_PENALTY_3=0.2060
PRIMER_LEFT_3_PENALTY=0.016975
PRIMER_RIGHT_3_PENALTY=0.189072
PRIMER_LEFT_3_SEQUENCE=AGGCCAGTCTTCCTACAGCTACT
PRIMER_RIGHT_3_SEQUENCE=TCCTCCTCTTCAGGAAGAAAATC
PRIMER_LEFT_3=1549,23
PRIMER_RIGHT_3=2384,23
PRIMER_LEFT_3_TM=59.983
PRIMER_RIGHT_3_TM=60.189
PRIMER_LEFT_3_GC_PERCENT=52.174
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_3_MISPRIMING_SCORE=24.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_3_END_STABILITY=5.4000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=836
PRIMER_PAIR_PENALTY_4=0.2082
PRIMER_LEFT_4_PENALTY=0.137575
PRIMER_RIGHT_4_PENALTY=0.070598
PRIMER_LEFT_4_SEQUENCE=GAAGCACAGCACACACTAACATC
PRIMER_RIGHT_4_SEQUENCE=TCTCCAGTGTACCCTACCCAGTA
PRIMER_LEFT_4=537,23
PRIMER_RIGHT_4=1323,23
PRIMER_LEFT_4_TM=59.862
PRIMER_RIGHT_4_TM=59.929
PRIMER_LEFT_4_GC_PERCENT=47.826
PRIMER_RIGHT_4_GC_PERCENT=52.174
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, reverse MER4C a consensus
PRIMER_RIGHT_4_MISPRIMING_SCORE=11.00, L1MD1 3'-end of L1 repeat (subfamily L1MD1) - a consensus sequence
PRIMER_PAIR_4_MISPRIMING_SCORE=22.00, reverse MER4C a consensus
PRIMER_LEFT_4_END_STABILITY=6.3000
PRIMER_RIGHT_4_END_STABILITY=5.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=787
PRIMER_PAIR_PENALTY_5=0.2164
PRIMER_LEFT_5_PENALTY=0.016975
PRIMER_RIGHT_5_PENALTY=0.199411
PRIMER_LEFT_5_SEQUENCE=AGGCCAGTCTTCCTACAGCTACT
PRIMER_RIGHT_5_SEQUENCE=AGCTAAAGACCAGATCCCTGAGT
PRIMER_LEFT_5=1549,23
PRIMER_RIGHT_5=2432,23
PRIMER_LEFT_5_TM=59.983
PRIMER_RIGHT_5_TM=59.801
PRIMER_LEFT_5_GC_PERCENT=52.174
PRIMER_RIGHT_5_GC_PERCENT=47.826
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=2.00
PRIMER_RIGHT_5_SELF_END=2.00
PRIMER_LEFT_5_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_5_MISPRIMING_SCORE=12.00, MSTa consensus sequence
PRIMER_PAIR_5_MISPRIMING_SCORE=24.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_5_END_STABILITY=5.4000
PRIMER_RIGHT_5_END_STABILITY=6.4000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_5=884
PRIMER_PAIR_PENALTY_6=0.2182
PRIMER_LEFT_6_PENALTY=0.016975
PRIMER_RIGHT_6_PENALTY=0.201271
PRIMER_LEFT_6_SEQUENCE=AGGCCAGTCTTCCTACAGCTACT
PRIMER_RIGHT_6_SEQUENCE=AGAGTGAGACACGACCTTCATTC
PRIMER_LEFT_6=1549,23
PRIMER_RIGHT_6=2278,23
PRIMER_LEFT_6_TM=59.983
PRIMER_RIGHT_6_TM=59.799
PRIMER_LEFT_6_GC_PERCENT=52.174
PRIMER_RIGHT_6_GC_PERCENT=47.826
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=2.00
PRIMER_RIGHT_6_SELF_END=2.00
PRIMER_LEFT_6_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_6_MISPRIMING_SCORE=11.00, LTR3 LTR from human DNA related to mouse mammary tumor virus (MMTV) 3' LTR
PRIMER_PAIR_6_MISPRIMING_SCORE=20.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_6_END_STABILITY=5.4000
PRIMER_RIGHT_6_END_STABILITY=6.9000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_6=730
PRIMER_PAIR_PENALTY_7=0.2202
PRIMER_LEFT_7_PENALTY=0.203207
PRIMER_RIGHT_7_PENALTY=0.016975
PRIMER_LEFT_7_SEQUENCE=GAGACCCGAAACACAACAAATAC
PRIMER_RIGHT_7_SEQUENCE=AGTAGCTGTAGGAAGACTGGCCT
PRIMER_LEFT_7=1337,23
PRIMER_RIGHT_7=1571,23
PRIMER_LEFT_7_TM=59.797
PRIMER_RIGHT_7_TM=59.983
PRIMER_LEFT_7_GC_PERCENT=43.478
PRIMER_RIGHT_7_GC_PERCENT=52.174
PRIMER_LEFT_7_SELF_ANY=2.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=3.00
PRIMER_LEFT_7_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_7_MISPRIMING_SCORE=12.00, reverse PTR5 Human PTR5 mRNA for repetitive sequence
PRIMER_PAIR_7_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_7_END_STABILITY=5.6000
PRIMER_RIGHT_7_END_STABILITY=10.9000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_7=235
PRIMER_PAIR_PENALTY_8=0.2303
PRIMER_LEFT_8_PENALTY=0.088390
PRIMER_RIGHT_8_PENALTY=0.141958
PRIMER_LEFT_8_SEQUENCE=AGACACAGTCACCATCACTTCCT
PRIMER_RIGHT_8_SEQUENCE=GCTAAAGACCAGATCCCTGAGTT
PRIMER_LEFT_8=2041,23
PRIMER_RIGHT_8=2431,23
PRIMER_LEFT_8_TM=60.088
PRIMER_RIGHT_8_TM=60.142
PRIMER_LEFT_8_GC_PERCENT=47.826
PRIMER_RIGHT_8_GC_PERCENT=47.826
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_MISPRIMING_SCORE=12.00, reverse MER4C a consensus
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, reverse L1MD1 3'-end of L1 repeat (subfamily L1MD1) - a consensus sequence
PRIMER_PAIR_8_MISPRIMING_SCORE=21.00, reverse L1MD1 3'-end of L1 repeat (subfamily L1MD1) - a consensus sequence
PRIMER_LEFT_8_END_STABILITY=8.2000
PRIMER_RIGHT_8_END_STABILITY=6.4000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_8=391
PRIMER_PAIR_PENALTY_9=0.2386
PRIMER_LEFT_9_PENALTY=0.049500
PRIMER_RIGHT_9_PENALTY=0.189072
PRIMER_LEFT_9_SEQUENCE=CTTCCTACAGCTACTGGGTCTCA
PRIMER_RIGHT_9_SEQUENCE=TCCTCCTCTTCAGGAAGAAAATC
PRIMER_LEFT_9=1557,23
PRIMER_RIGHT_9=2384,23
PRIMER_LEFT_9_TM=59.950
PRIMER_RIGHT_9_TM=60.189
PRIMER_LEFT_9_GC_PERCENT=52.174
PRIMER_RIGHT_9_GC_PERCENT=43.478
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=8.00
PRIMER_LEFT_9_SELF_END=3.00
PRIMER_RIGHT_9_SELF_END=2.00
PRIMER_LEFT_9_MISPRIMING_SCORE=12.00, MER43 Homo sapiens clone DOE1-35 MER repetitive sequence - a consensus
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_9_MISPRIMING_SCORE=24.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_9_END_STABILITY=6.7000
PRIMER_RIGHT_9_END_STABILITY=6.9000
PRIMER_PAIR_9_COMPL_ANY=6.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_9=828
PRIMER_PAIR_PENALTY_10=0.2453
PRIMER_LEFT_10_PENALTY=0.016975
PRIMER_RIGHT_10_PENALTY=0.228317
PRIMER_LEFT_10_SEQUENCE=AGGCCAGTCTTCCTACAGCTACT
PRIMER_RIGHT_10_SEQUENCE=ACAGAGTGAGACACGACCTTCAT
PRIMER_LEFT_10=1549,23
PRIMER_RIGHT_10=2280,23
PRIMER_LEFT_10_TM=59.983
PRIMER_RIGHT_10_TM=60.228
PRIMER_LEFT_10_GC_PERCENT=52.174
PRIMER_RIGHT_10_GC_PERCENT=47.826
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=2.00
PRIMER_RIGHT_10_SELF_END=2.00
PRIMER_LEFT_10_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_10_MISPRIMING_SCORE=11.00, MER7B Nonautonomous DNA transposon
PRIMER_PAIR_10_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_10_END_STABILITY=5.4000
PRIMER_RIGHT_10_END_STABILITY=6.9000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_10=732
PRIMER_PAIR_PENALTY_11=0.2489
PRIMER_LEFT_11_PENALTY=0.049500
PRIMER_RIGHT_11_PENALTY=0.199411
PRIMER_LEFT_11_SEQUENCE=CTTCCTACAGCTACTGGGTCTCA
PRIMER_RIGHT_11_SEQUENCE=AGCTAAAGACCAGATCCCTGAGT
PRIMER_LEFT_11=1557,23
PRIMER_RIGHT_11=2432,23
PRIMER_LEFT_11_TM=59.950
PRIMER_RIGHT_11_TM=59.801
PRIMER_LEFT_11_GC_PERCENT=52.174
PRIMER_RIGHT_11_GC_PERCENT=47.826
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=3.00
PRIMER_RIGHT_11_SELF_END=2.00
PRIMER_LEFT_11_MISPRIMING_SCORE=12.00, MER43 Homo sapiens clone DOE1-35 MER repetitive sequence - a consensus
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, MSTa consensus sequence
PRIMER_PAIR_11_MISPRIMING_SCORE=24.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_11_END_STABILITY=6.7000
PRIMER_RIGHT_11_END_STABILITY=6.4000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_11=876
PRIMER_PAIR_PENALTY_12=0.2508
PRIMER_LEFT_12_PENALTY=0.049500
PRIMER_RIGHT_12_PENALTY=0.201271
PRIMER_LEFT_12_SEQUENCE=CTTCCTACAGCTACTGGGTCTCA
PRIMER_RIGHT_12_SEQUENCE=AGAGTGAGACACGACCTTCATTC
PRIMER_LEFT_12=1557,23
PRIMER_RIGHT_12=2278,23
PRIMER_LEFT_12_TM=59.950
PRIMER_RIGHT_12_TM=59.799
PRIMER_LEFT_12_GC_PERCENT=52.174
PRIMER_RIGHT_12_GC_PERCENT=47.826
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=3.00
PRIMER_RIGHT_12_SELF_END=2.00
PRIMER_LEFT_12_MISPRIMING_SCORE=12.00, MER43 Homo sapiens clone DOE1-35 MER repetitive sequence - a consensus
PRIMER_RIGHT_12_MISPRIMING_SCORE=11.00, LTR3 LTR from human DNA related to mouse mammary tumor virus (MMTV) 3' LTR
PRIMER_PAIR_12_MISPRIMING_SCORE=21.00, MER43 Homo sapiens clone DOE1-35 MER repetitive sequence - a consensus
PRIMER_LEFT_12_END_STABILITY=6.7000
PRIMER_RIGHT_12_END_STABILITY=6.9000
PRIMER_PAIR_12_COMPL_ANY=6.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_12=722
PRIMER_PAIR_PENALTY_13=0.2525
PRIMER_LEFT_13_PENALTY=0.016975
PRIMER_RIGHT_13_PENALTY=0.235561
PRIMER_LEFT_13_SEQUENCE=AGGCCAGTCTTCCTACAGCTACT
PRIMER_RIGHT_13_SEQUENCE=ATAGCCTCTGACCTCATTCCTCT
PRIMER_LEFT_13=1549,23
PRIMER_RIGHT_13=1711,23
PRIMER_LEFT_13_TM=59.983
PRIMER_RIGHT_13_TM=59.764
PRIMER_LEFT_13_GC_PERCENT=52.174
PRIMER_RIGHT_13_GC_PERCENT=47.826
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=2.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_13_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_13_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_13_END_STABILITY=5.4000
PRIMER_RIGHT_13_END_STABILITY=7.9000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_13=163
PRIMER_PAIR_PENALTY_14=0.2527
PRIMER_LEFT_14_PENALTY=0.203207
PRIMER_RIGHT_14_PENALTY=0.049500
PRIMER_LEFT_14_SEQUENCE=GAGACCCGAAACACAACAAATAC
PRIMER_RIGHT_14_SEQUENCE=TGAGACCCAGTAGCTGTAGGAAG
PRIMER_LEFT_14=1337,23
PRIMER_RIGHT_14=1579,23
PRIMER_LEFT_14_TM=59.797
PRIMER_RIGHT_14_TM=59.950
PRIMER_LEFT_14_GC_PERCENT=43.478
PRIMER_RIGHT_14_GC_PERCENT=52.174
PRIMER_LEFT_14_SELF_ANY=2.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_14_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_14_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_14_END_STABILITY=5.6000
PRIMER_RIGHT_14_END_STABILITY=8.2000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_14=243
PRIMER_PAIR_PENALTY_15=0.2596
PRIMER_LEFT_15_PENALTY=0.137575
PRIMER_RIGHT_15_PENALTY=0.122060
PRIMER_LEFT_15_SEQUENCE=GAAGCACAGCACACACTAACATC
PRIMER_RIGHT_15_SEQUENCE=ATATGGAGGTTTCTCACTGGGTT
PRIMER_LEFT_15=537,23
PRIMER_RIGHT_15=1227,23
PRIMER_LEFT_15_TM=59.862
PRIMER_RIGHT_15_TM=60.122
PRIMER_LEFT_15_GC_PERCENT=47.826
PRIMER_RIGHT_15_GC_PERCENT=43.478
PRIMER_LEFT_15_SELF_ANY=2.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_MISPRIMING_SCORE=12.00, reverse MER4C a consensus
PRIMER_RIGHT_15_MISPRIMING_SCORE=12.00, L1PA11 3'-end of L1 repeat (subfamily L1PA11) - a consensus sequence
PRIMER_PAIR_15_MISPRIMING_SCORE=22.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_15_END_STABILITY=6.3000
PRIMER_RIGHT_15_END_STABILITY=9.4000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_15=691
PRIMER_PAIR_PENALTY_16=0.2599
PRIMER_LEFT_16_PENALTY=0.133805
PRIMER_RIGHT_16_PENALTY=0.126080
PRIMER_LEFT_16_SEQUENCE=CCCTTCAGCTTTGTAAGGAAGAT
PRIMER_RIGHT_16_SEQUENCE=CCGACCCCAGATTATAGAAGACT
PRIMER_LEFT_16=3188,23
PRIMER_RIGHT_16=3805,23
PRIMER_LEFT_16_TM=60.134
PRIMER_RIGHT_16_TM=59.874
PRIMER_LEFT_16_GC_PERCENT=43.478
PRIMER_RIGHT_16_GC_PERCENT=47.826
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=3.00
PRIMER_RIGHT_16_SELF_END=3.00
PRIMER_LEFT_16_MISPRIMING_SCORE=12.00, MER4C a consensus
PRIMER_RIGHT_16_MISPRIMING_SCORE=11.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_16_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_16_END_STABILITY=6.6000
PRIMER_RIGHT_16_END_STABILITY=6.1000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_16=618
PRIMER_PAIR_PENALTY_17=0.2728
PRIMER_LEFT_17_PENALTY=0.088390
PRIMER_RIGHT_17_PENALTY=0.184405
PRIMER_LEFT_17_SEQUENCE=AGACACAGTCACCATCACTTCCT
PRIMER_RIGHT_17_SEQUENCE=ACTTCCTCACCTACCAGGGTTAC
PRIMER_LEFT_17=2041,23
PRIMER_RIGHT_17=2907,23
PRIMER_LEFT_17_TM=60.088
PRIMER_RIGHT_17_TM=59.816
PRIMER_LEFT_17_GC_PERCENT=47.826
PRIMER_RIGHT_17_GC_PERCENT=52.174
PRIMER_LEFT_17_SELF_ANY=3.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=3.00
PRIMER_LEFT_17_MISPRIMING_SCORE=12.00, reverse MER4C a consensus
PRIMER_RIGHT_17_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_17_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_17_END_STABILITY=8.2000
PRIMER_RIGHT_17_END_STABILITY=5.4000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_17=867
PRIMER_PAIR_PENALTY_18=0.2758
PRIMER_LEFT_18_PENALTY=0.141958
PRIMER_RIGHT_18_PENALTY=0.133805
PRIMER_LEFT_18_SEQUENCE=AACTCAGGGATCTGGTCTTTAGC
PRIMER_RIGHT_18_SEQUENCE=ATCTTCCTTACAAAGCTGAAGGG
PRIMER_LEFT_18=2409,23
PRIMER_RIGHT_18=3210,23
PRIMER_LEFT_18_TM=60.142
PRIMER_RIGHT_18_TM=60.134
PRIMER_LEFT_18_GC_PERCENT=47.826
PRIMER_RIGHT_18_GC_PERCENT=43.478
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=2.00
PRIMER_RIGHT_18_SELF_END=2.00
PRIMER_LEFT_18_MISPRIMING_SCORE=12.00, reverse L1MD1 3'-end of L1 repeat (subfamily L1MD1) - a consensus sequence
PRIMER_RIGHT_18_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_18_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_18_END_STABILITY=7.5000
PRIMER_RIGHT_18_END_STABILITY=9.7000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_18=802
PRIMER_PAIR_PENALTY_19=0.2775
PRIMER_LEFT_19_PENALTY=0.088390
PRIMER_RIGHT_19_PENALTY=0.189072
PRIMER_LEFT_19_SEQUENCE=AGACACAGTCACCATCACTTCCT
PRIMER_RIGHT_19_SEQUENCE=TCCTCCTCTTCAGGAAGAAAATC
PRIMER_LEFT_19=2041,23
PRIMER_RIGHT_19=2384,23
PRIMER_LEFT_19_TM=60.088
PRIMER_RIGHT_19_TM=60.189
PRIMER_LEFT_19_GC_PERCENT=47.826
PRIMER_RIGHT_19_GC_PERCENT=43.478
PRIMER_LEFT_19_SELF_ANY=3.00
PRIMER_RIGHT_19_SELF_ANY=8.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=2.00
PRIMER_LEFT_19_MISPRIMING_SCORE=12.00, reverse MER4C a consensus
PRIMER_RIGHT_19_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_19_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_19_END_STABILITY=8.2000
PRIMER_RIGHT_19_END_STABILITY=6.9000
PRIMER_PAIR_19_COMPL_ANY=6.00
PRIMER_PAIR_19_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_19=344
=