PRIMER_SEQUENCE_ID=NM_002844
SEQUENCE=CGAGCATGGAGAGCTGCCGCCGCGGCCGGCCGGCGACGCTGGCGACGCTTTCGCCCCTGAGGTAGTTTGGCGACCGCGAAGAAGGAAAAAGGGCGGGCGGGCGGCTGTCCTCTCACCGTCCTCACCCCGCGAGGCCCGGCCCGCTCCTCCGTCGTGGATTTCGCGGCGATCCCCCCGGCAGCTCTTTGCAAAGCTGCTTGAAACTTCTCCCAAACTCGGCATGGATACGACTGCGGCGGCGGCGCTGCCTGCTTTTGTGGCGCTCTTGCTCCTCTCTCCTTGGCCTCTCCTGGGATCGGCCCAAGGCCAGTTCTCCGCAGGTGGCTGTACTTTTGATGATGGTCCAGGGGCCTGTGATTACCACCAGGATCTGTATGATGACTTTGAATGGGTGCATGTTAGTGCTCAAGAGCCTCATTATCTACCACCCGAGATGCCCCAAGGTTCCTATATGATAGTGGACTCTTCAGATCACGACCCTGGAGAAAAAGCCAGACTTCAGCTGCCTACAATGAAGGAGAACGACACTCACTGCATTGATTTCAGTTACCTATTATATAGCCAGAAAGGACTGAATCCTGGCACTTTGAACATATTAGTTAGGGTGAATAAAGGACCTCTTGCCAATCCAATTTGGAATGTGACTGGATTCACGGGTAGAGATTGGCTTCGGGCTGAGCTAGCAGTGAGCACCTTTTGGCCCAATGAATATCAGGTAATATTTGAAGCTGAAGTCTCAGGAGGGAGAAGTGGTTATATTGCCATTGATGACATCCAAGTACTGAGTTATCCTTGTGATAAATCTCCTCATTTCCTCCGTCTAGGGGATGTAGAGGTGAATGCAGGGCAAAACGCTACATTTCAGTGCATTGCCACAGGGAGAGATGCTGTGCATAACAAGTTATGGCTCCAGAGACGAAATGGAGAAGATATACCAGTAGCCCAGACTAAGAACATCAATCATAGAAGGTTTGCCGCTTCCTTCAGATTGCAAGAAGTGACAAAAACTGACCAGGATTTGTATCGCTGTGTAACTCAGTCAGAACGAGGTTCCGGTGTGTCCAATTTTGCTCAACTTATTGTGAGAGAACCGCCAAGACCCATTGCTCCTCCTCAGCTTCTTGGTGTTGGGCCTACATATTTGCTGATCCAACTAAATGCCAACTCGATCATTGGCGATGGTCCTATCATCCTGAAAGAAGTAGAGTACCGAATGACATCAGGATCCTGGACAGAAACCCATGCAGTCAATGCTCCAACTTACAAATTATGGCATTTAGATCCAGATACCGAATATGAGATCCGAGTTCTACTTACAAGACCTGGTGAAGGTGGAACGGGGCTCCCAGGACCTCCACTAATCACCAGAACAAAATGTGCAGAACCTATGAGAACCCCAAAGACATTAAAGATTGCTGAAATACAGGCAAGACGGATTGCTGTGGACTGGGAATCCTTGGGTTACAACATTACGCGTTGCCACACTTTTAATGTCACTATCTGCTACCATTACTTCCGTGGTCACAACGAGAGCAAGGCAGACTGTTTGGACATGGACCCCAAAGCCCCTCAGCATGTTGTGAACCATCTGCCACCTTATACAAATGTCAGCCTCAAGATGATCCTAACCAATCCAGAGGGAAGGAAGGAGAGTGAAGAGACAATTATTCAAACTGATGAAGATGTGCCTGGTCCCGTACCAGTAAAATCTCTTCAAGGAACATCCTTTGAAAATAAGATCTTCTTGAACTGGAAAGAACCTTTGGATCCAAATGGAATCATCACTCAATATGAGATCAGCTATAGCAGTATAAGATCATTTGATCCTGCAGTTCCAGTGGCTGGACCTCCCCAGACTGTATCAAATTTATGGAACAGTACACACCATGTCTTTATGCATCTCCACCCTGGAACCACGTACCAGTTTTTCATAAGAGCCAGCACGGTCAAAGGCTTTGGTCCAGCCACAGCCATCAATGTCACCACCAATATCTCAGCTCCAACTTTACCTGACTATGAAGGAGTTGATGCCTCTCTCAATGAAACTGCCACCACAATAACTGTATTGTTGAGACCAGCACAAGCCAAAGGTGCTCCTATCAGTGCTTATCAGATTGTTGTGGAAGAACTGCACCCACACCGAACCAAGAGAGAAGCCGGAGCCATGGAATGCTACCAGGTTCCTGTCACATACCAAAATGCCATGAGTGGGGGTGCACCGTATTACTTTGCTGCAGAACTCCCCCCGGGAAACCTACCTGAGCCTGCCCCGTTCACTGTGGGTGACAATCGGACCTACCAAGGCTTTTGGAACCCTCCTTTGGCTCCGCGCAAAGGATACAACATCTATTTCCAGGCGATGAGCAGTGTGGAGAAGGAAACTAAAACCCAGTGCGTACGCATTGCTACAAAAGCAGCAGCAACAGAAGAACCAGAAGTGATCCCAGATCCCGCCAAGCAGACAGACAGAGTGGTGAAAATAGCAGGAATTAGTGCTGGAATTTTGGTGTTCATCCTCCTTCTCCTAGTTGTCATATTAATTGTAAAAAAGAGCAAACTTGCTAAAAAACGCAAAGATGCCATGGGGAATACCCGGCAGGAGATGACTCACATGGTGAATGCAATGGATCGAAGTTATGCTGATCAGAGCACTCTGCATGCAGAAGATCCTCTTTCCATCACCTTCATGGACCAACATAACTTTAGTCCAAGATATGAGAACCACAGTGCTACAGCAGAGTCCAGTCGCCTTCTAGACGTACCTCGCTACCTCTGTGAGGGGACGGAATCCCCTTACCAGACAGGACAGCTGCATCCAGCCATCAGGGTAGCTGATTTACTGCAGCACATTAATCTCATGAAGACATCAGACAGCTATGGGTTCAAAGAGGAATATGAGAGCTTTTTTGAAGGACAGTCAGCATCTTGGGATGTAGCTAAAAAAGATCAAAATAGAGCAAAAAACCGATATGGAAACATTATAGCATATGATCACTCCAGAGTGATTTTGCAACCCGTAGAGGATGATCCTTCCTCAGATTATATTAATGCCAACTATATTGATGGCTACCAGAGACCAAGTCATTACATTGCAACCCAAGGTCCCGTTCATGAAACAGTGTATGATTTCTGGAGGATGATTTGGCAAGAACAATCTGCTTGCATTGTGATGGTTACAAATTTAGTTGAGGTTGGCCGGGTTAAATGCTATAAATATTGGCCTGATGATACTGAAGTTTATGGTGACTTCAAAGTAACGTGTGTAGAAATGGAACCACTTGCTGAATATGTAGTTAGGACATTCACCCTGGAAAGGAGGGGGTACAATGAAATCCGTGAAGTTAAACAGTTCCATTTCACGGGCTGGCCTGACCATGGAGTGCCCTACCATGCTACAGGGCTGCTTTCCTTTATCCGGCGAGTCAAGTTATCAAACCCTCCCAGTGCTGGCCCCATCGTTGTACATTGCAGTGCTGGTGCTGGACGAACTGGCTGCTACATTGTGATTGACATCATGCTAGACATGGCTGAAAGAGAGGGTGTTGTTGATATTTACAATTGTGTCAAAGCCTTAAGATCTCGGCGTATTAATATGGTCCAGACAGAGGAACAGTACATTTTTATTCATGATGCCATTTTAGAAGCCTGCTTATGTGGAGAAACTGCCATACCTGTCTGTGAATTTAAAGCTGCATATTTTGATATGATTAGAATAGACTCCCAGACTAACTCTTCACATCTCAAGGATGAATTTCAGACTCTGAATTCAGTCACCCCTCGACTACAAGCTGAAGACTGCAGTATAGCGTGCCTGCCAAGGAACCATGACAAGAACCGTTTCATGGACATGCTGCCACCTGACAGATGTCTGCCTTTTTTAATTACAATTGATGGGGAGAGCAGTAACTACATCAATGCTGCTCTTATGGACAGCTACAGGCAACCAGCTGCTTTCATCGTCACACAATACCCTCTGCCAAACACTGTAAAAGACTTCTGGAGATTAGTGTATGATTATGGCTGTACCTCCATTGTGATGTTAAACGAAGTCGACTTGTCCCAGGGCTGCCCTCAGTACTGGCCAGAGGAAGGGATGCTACGATATGGCCCCATCCAAGTGGAATGTATGTCTTGTTCAATGGACTGTGATGTGATCAACCGGATTTTTAGGATATGCAATCTAACAAGACCACAGGAAGGTTATCTGATGGTGCAACAGTTTCAGTACCTAGGATGGGCTTCTCATCGAGAAGTGCCTGGATCCAAAAGGTCATTCTTGAAACTGATACTTCAGGTGGAAAAGTGGCAGGAGGAATGCGAGGAAGGGGAAGGCCGGACGATTATCCACTGCCTAAATGGTGGCGGGCGAAGTGGCATGTTCTGTGCTATAGGCATCGTTGTTGAAATGGTGAAACGGCAAAATGTTGTCGATGTTTTCCATGCAGTAAAGACACTGAGGAACAGCAAGCCAAACATGGTGGAAGCCCCGGAGCAATACCGTTTCTGCTATGATGTAGCTTTGGAGTACCTGGAATCATCTTAGTTGGGTGAGACTCTTTAAAGTGCATCCATGAAGAAACCTGTCCATCTATTGAGCCAGCAGCTGTTGTACCTGTTACACTTGTGCAGAAAGATTTTAATGTGGGGGGTGGGAGACTTTTACATTTGAGAGGTAAAAGTATTTTTTTTATGAAGTTGTGTATCTTAATAAAAAGGACTGAATTAGTTTTTATTACTATATTAAAGCATCAACATTTCATGCCACATAAATTATATTTAATAAGAACCAGATTGAAATGAGAACGTATTGGTGTTTGTACAGTGAACATGCCACCTTTTTTCTCATGGTTTCAGTAGAGCAGCTACCACATGTTGCATGAGTTCATACTTTCTACGTGGCATTTTTCTCCCTTTCTAAAATGAAAGCTGATGAATCTTAAAAGGAAGAAGAAAAGAAAAGCTGTGCAAATTCATAGTAAAGTTCGTTTTTTATATGTTTCCAGTGTAGCAGATCTCTATATAAATATATAAATATATATAACTGGCTTATTTTCTTTTAATGTGCAATGATGGCTGGATCATTTAAAGTTCTTTTTAGAAAATAACATAAGCCAAAGACTCAAGTGTAAATATGTCTATATGGAGAAAGCACATTATATTTATTGGTTACTTACATTCCTTTTTTGATGGCTAAAATACTACCACCACACAATCATCTTTTTTTCCTGAAGAAAGCTTTTTCTTTAGCTAAAATCAATTGTAAACGATTTTTGTAGATTATTTTTTGTATGTTTTAGTGTAAGTAGAAGATAAACTTTTTATTCATAAACCAGGAAGCAATGTTCTTTATAGTGATTCTCTTGTGTACATGCTTGTGAATTAAATTTGTGTAAAATCCCTTGGCAATTGGGTCTTTTAATATAGGACCAAATTAAAACATTTTGCTGAATATGTATAGTTTTTCACAATTTCATTAGGTAAATAATGGTTTGGTGATCATACATGAGAAATGTACACATTAAAAGGCCTTGCTGACAACTTGCACAATGTTGAACATAGCCTTTAAGCATCATTTAAATTTTAAAGGAATGGAGTTTTTCAGCCTGTGGCCCAGCACTGGTCAAGAAAACAAGATGGCAACATATATGCTTTCAGGGTCAAATTTGAGCAAACTGTAAACTGTCAGGGTGATAAAATGTTTCTCTTGATGTTTACATGCACAAGCTTTGCGTTCTGACTATAAAAAGTGTGAACAAATCAATGCCAGATTCCTGTTTTGCGCATTGTCATGGGATTCTTAAGTGAACCTTTCTAAATGTGGTCTTGTTCACATGCTCCACGTAGCTGTAACTTCACATCATCAGCTTGCAGTTTGTAATTGACTAAAGCATTCCAGTGTCCTCTTTCTAGATTGCCAGCTCATGACATGGTGCTTATAAAGATTTAATTAAAGTAAGAATGAAATAAAGTTTTTATAATTAT
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 68385, GC content failed 2477, low tm 34293, high tm 16291, high any compl 3, high end compl 27, high repeat similarity 38, long poly-x seq 302, ok 14954
PRIMER_RIGHT_EXPLAIN=considered 68417, GC content failed 2618, low tm 34572, high tm 15878, high end compl 8, high repeat similarity 9, long poly-x seq 299, ok 15033
PRIMER_PAIR_EXPLAIN=considered 853, unacceptable product size 787, high end compl 5, ok 61
PRIMER_PAIR_PENALTY=0.0246
PRIMER_LEFT_PENALTY=0.014220
PRIMER_RIGHT_PENALTY=0.010384
PRIMER_LEFT_SEQUENCE=CTGATCCAACTAAATGCCAACTC
PRIMER_RIGHT_SEQUENCE=CAGCAATCTTTAATGTCTTTGGG
PRIMER_LEFT=1144,23
PRIMER_RIGHT=1417,23
PRIMER_LEFT_TM=60.014
PRIMER_RIGHT_TM=60.010
PRIMER_LEFT_GC_PERCENT=43.478
PRIMER_RIGHT_GC_PERCENT=39.130
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_MISPRIMING_SCORE=21.00, L1MC2 3'-end of L1 repeat (subfamily L1MC2) - a consensus sequence
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=10.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=274
PRIMER_PAIR_PENALTY_1=0.0246
PRIMER_LEFT_1_PENALTY=0.010384
PRIMER_RIGHT_1_PENALTY=0.014220
PRIMER_LEFT_1_SEQUENCE=CAGGAATTAGTGCTGGAATTTTG
PRIMER_RIGHT_1_SEQUENCE=AAACTTCAGTATCATCAGGCCAA
PRIMER_LEFT_1=2483,23
PRIMER_RIGHT_1=3238,23
PRIMER_LEFT_1_TM=60.010
PRIMER_RIGHT_1_TM=60.014
PRIMER_LEFT_1_GC_PERCENT=39.130
PRIMER_RIGHT_1_GC_PERCENT=39.130
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_MISPRIMING_SCORE=11.00, MER4C a consensus
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, LTR7 LTR from human endogenous retrovirus RTVL-H2
PRIMER_PAIR_1_MISPRIMING_SCORE=23.00, reverse L1MB7 3'-end of L1 repeat (subfamily L1MB7) - a consensus sequence
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=10.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=756
PRIMER_PAIR_PENALTY_2=0.0385
PRIMER_LEFT_2_PENALTY=0.010384
PRIMER_RIGHT_2_PENALTY=0.028128
PRIMER_LEFT_2_SEQUENCE=CAGGAATTAGTGCTGGAATTTTG
PRIMER_RIGHT_2_SEQUENCE=CAGTAAATCAGCTACCCTGATGG
PRIMER_LEFT_2=2483,23
PRIMER_RIGHT_2=2841,23
PRIMER_LEFT_2_TM=60.010
PRIMER_RIGHT_2_TM=60.028
PRIMER_LEFT_2_GC_PERCENT=39.130
PRIMER_RIGHT_2_GC_PERCENT=47.826
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_MISPRIMING_SCORE=11.00, MER4C a consensus
PRIMER_RIGHT_2_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_2_MISPRIMING_SCORE=20.00, MIR2 Repetitive element - a consensus
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=359
PRIMER_PAIR_PENALTY_3=0.0671
PRIMER_LEFT_3_PENALTY=0.045432
PRIMER_RIGHT_3_PENALTY=0.021648
PRIMER_LEFT_3_SEQUENCE=ATGGTCCAGACAGAGGAACAGTA
PRIMER_RIGHT_3_SEQUENCE=CAGTTTCAAGAATGACCTTTTGG
PRIMER_LEFT_3=3592,23
PRIMER_RIGHT_3=4284,23
PRIMER_LEFT_3_TM=60.045
PRIMER_RIGHT_3_TM=60.022
PRIMER_LEFT_3_GC_PERCENT=47.826
PRIMER_RIGHT_3_GC_PERCENT=39.130
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, MSTc consensus sequence
PRIMER_PAIR_3_MISPRIMING_SCORE=22.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_3_END_STABILITY=5.7000
PRIMER_RIGHT_3_END_STABILITY=8.8000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=693
PRIMER_PAIR_PENALTY_4=0.0671
PRIMER_LEFT_4_PENALTY=0.045432
PRIMER_RIGHT_4_PENALTY=0.021648
PRIMER_LEFT_4_SEQUENCE=TATGGTCCAGACAGAGGAACAGT
PRIMER_RIGHT_4_SEQUENCE=CAGTTTCAAGAATGACCTTTTGG
PRIMER_LEFT_4=3591,23
PRIMER_RIGHT_4=4284,23
PRIMER_LEFT_4_TM=60.045
PRIMER_RIGHT_4_TM=60.022
PRIMER_LEFT_4_GC_PERCENT=47.826
PRIMER_RIGHT_4_GC_PERCENT=39.130
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, MSTc consensus sequence
PRIMER_PAIR_4_MISPRIMING_SCORE=21.00, MER21B a consensus
PRIMER_LEFT_4_END_STABILITY=6.1000
PRIMER_RIGHT_4_END_STABILITY=8.8000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=694
PRIMER_PAIR_PENALTY_5=0.0714
PRIMER_LEFT_5_PENALTY=0.021648
PRIMER_RIGHT_5_PENALTY=0.049761
PRIMER_LEFT_5_SEQUENCE=CCAAAAGGTCATTCTTGAAACTG
PRIMER_RIGHT_5_SEQUENCE=TTTGCACAGCTTTTCTTTTCTTC
PRIMER_LEFT_5=4262,23
PRIMER_RIGHT_5=4968,23
PRIMER_LEFT_5_TM=60.022
PRIMER_RIGHT_5_TM=60.050
PRIMER_LEFT_5_GC_PERCENT=39.130
PRIMER_RIGHT_5_GC_PERCENT=34.783
PRIMER_LEFT_5_SELF_ANY=5.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=3.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_5_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_5_MISPRIMING_SCORE=23.00, Tigger2 Autonomous DNA transposon
PRIMER_LEFT_5_END_STABILITY=6.7000
PRIMER_RIGHT_5_END_STABILITY=6.7000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_5=707
PRIMER_PAIR_PENALTY_6=0.0718
PRIMER_LEFT_6_PENALTY=0.022025
PRIMER_RIGHT_6_PENALTY=0.049761
PRIMER_LEFT_6_SEQUENCE=ATGGTGCAACAGTTTCAGTACCT
PRIMER_RIGHT_6_SEQUENCE=TTTGCACAGCTTTTCTTTTCTTC
PRIMER_LEFT_6=4207,23
PRIMER_RIGHT_6=4968,23
PRIMER_LEFT_6_TM=59.978
PRIMER_RIGHT_6_TM=60.050
PRIMER_LEFT_6_GC_PERCENT=43.478
PRIMER_RIGHT_6_GC_PERCENT=34.783
PRIMER_LEFT_6_SELF_ANY=7.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=3.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_MISPRIMING_SCORE=12.00, MER1B Nonautonomous DNA transposon
PRIMER_RIGHT_6_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_6_MISPRIMING_SCORE=22.00, reverse MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_LEFT_6_END_STABILITY=6.9000
PRIMER_RIGHT_6_END_STABILITY=6.7000
PRIMER_PAIR_6_COMPL_ANY=6.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_6=762
PRIMER_PAIR_PENALTY_7=0.0785
PRIMER_LEFT_7_PENALTY=0.014220
PRIMER_RIGHT_7_PENALTY=0.064245
PRIMER_LEFT_7_SEQUENCE=CTGATCCAACTAAATGCCAACTC
PRIMER_RIGHT_7_SEQUENCE=GTCAGGTAAAGTTGGAGCTGAGA
PRIMER_LEFT_7=1144,23
PRIMER_RIGHT_7=2013,23
PRIMER_LEFT_7_TM=60.014
PRIMER_RIGHT_7_TM=59.936
PRIMER_LEFT_7_GC_PERCENT=43.478
PRIMER_RIGHT_7_GC_PERCENT=47.826
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=5.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_7_MISPRIMING_SCORE=11.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_7_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_7_END_STABILITY=6.4000
PRIMER_RIGHT_7_END_STABILITY=6.7000
PRIMER_PAIR_7_COMPL_ANY=7.00
PRIMER_PAIR_7_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_7=870
PRIMER_PAIR_PENALTY_8=0.0809
PRIMER_LEFT_8_PENALTY=0.059210
PRIMER_RIGHT_8_PENALTY=0.021648
PRIMER_LEFT_8_SEQUENCE=ATACCCTCTGCCAAACACTGTAA
PRIMER_RIGHT_8_SEQUENCE=CAGTTTCAAGAATGACCTTTTGG
PRIMER_LEFT_8=3966,23
PRIMER_RIGHT_8=4284,23
PRIMER_LEFT_8_TM=59.941
PRIMER_RIGHT_8_TM=60.022
PRIMER_LEFT_8_GC_PERCENT=43.478
PRIMER_RIGHT_8_GC_PERCENT=39.130
PRIMER_LEFT_8_SELF_ANY=5.00
PRIMER_RIGHT_8_SELF_ANY=5.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=3.00
PRIMER_LEFT_8_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, MSTc consensus sequence
PRIMER_PAIR_8_MISPRIMING_SCORE=23.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_8_END_STABILITY=6.0000
PRIMER_RIGHT_8_END_STABILITY=8.8000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_8=319
PRIMER_PAIR_PENALTY_9=0.0809
PRIMER_LEFT_9_PENALTY=0.059210
PRIMER_RIGHT_9_PENALTY=0.021648
PRIMER_LEFT_9_SEQUENCE=AATACCCTCTGCCAAACACTGTA
PRIMER_RIGHT_9_SEQUENCE=CAGTTTCAAGAATGACCTTTTGG
PRIMER_LEFT_9=3965,23
PRIMER_RIGHT_9=4284,23
PRIMER_LEFT_9_TM=59.941
PRIMER_RIGHT_9_TM=60.022
PRIMER_LEFT_9_GC_PERCENT=43.478
PRIMER_RIGHT_9_GC_PERCENT=39.130
PRIMER_LEFT_9_SELF_ANY=5.00
PRIMER_RIGHT_9_SELF_ANY=5.00
PRIMER_LEFT_9_SELF_END=3.00
PRIMER_RIGHT_9_SELF_END=3.00
PRIMER_LEFT_9_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, MSTc consensus sequence
PRIMER_PAIR_9_MISPRIMING_SCORE=22.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_9_END_STABILITY=5.7000
PRIMER_RIGHT_9_END_STABILITY=8.8000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_9=320
PRIMER_PAIR_PENALTY_10=0.0833
PRIMER_LEFT_10_PENALTY=0.021648
PRIMER_RIGHT_10_PENALTY=0.061624
PRIMER_LEFT_10_SEQUENCE=CCAAAAGGTCATTCTTGAAACTG
PRIMER_RIGHT_10_SEQUENCE=ACGATGCCTATAGCACAGAACAT
PRIMER_LEFT_10=4262,23
PRIMER_RIGHT_10=4397,23
PRIMER_LEFT_10_TM=60.022
PRIMER_RIGHT_10_TM=60.062
PRIMER_LEFT_10_GC_PERCENT=39.130
PRIMER_RIGHT_10_GC_PERCENT=43.478
PRIMER_LEFT_10_SELF_ANY=5.00
PRIMER_RIGHT_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=3.00
PRIMER_RIGHT_10_SELF_END=2.00
PRIMER_LEFT_10_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_10_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_10_MISPRIMING_SCORE=24.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_10_END_STABILITY=6.7000
PRIMER_RIGHT_10_END_STABILITY=6.6000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_10=136
PRIMER_PAIR_PENALTY_11=0.0836
PRIMER_LEFT_11_PENALTY=0.022025
PRIMER_RIGHT_11_PENALTY=0.061624
PRIMER_LEFT_11_SEQUENCE=ATGGTGCAACAGTTTCAGTACCT
PRIMER_RIGHT_11_SEQUENCE=ACGATGCCTATAGCACAGAACAT
PRIMER_LEFT_11=4207,23
PRIMER_RIGHT_11=4397,23
PRIMER_LEFT_11_TM=59.978
PRIMER_RIGHT_11_TM=60.062
PRIMER_LEFT_11_GC_PERCENT=43.478
PRIMER_RIGHT_11_GC_PERCENT=43.478
PRIMER_LEFT_11_SELF_ANY=7.00
PRIMER_RIGHT_11_SELF_ANY=6.00
PRIMER_LEFT_11_SELF_END=3.00
PRIMER_RIGHT_11_SELF_END=2.00
PRIMER_LEFT_11_MISPRIMING_SCORE=12.00, MER1B Nonautonomous DNA transposon
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_11_MISPRIMING_SCORE=23.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_11_END_STABILITY=6.9000
PRIMER_RIGHT_11_END_STABILITY=6.6000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_11=191
PRIMER_PAIR_PENALTY_12=0.0860
PRIMER_LEFT_12_PENALTY=0.028128
PRIMER_RIGHT_12_PENALTY=0.057845
PRIMER_LEFT_12_SEQUENCE=CCATCAGGGTAGCTGATTTACTG
PRIMER_RIGHT_12_SEQUENCE=ATTCACAGACAGGTATGGCAGTT
PRIMER_LEFT_12=2819,23
PRIMER_RIGHT_12=3682,23
PRIMER_LEFT_12_TM=60.028
PRIMER_RIGHT_12_TM=59.942
PRIMER_LEFT_12_GC_PERCENT=47.826
PRIMER_RIGHT_12_GC_PERCENT=43.478
PRIMER_LEFT_12_SELF_ANY=6.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_12_MISPRIMING_SCORE=12.00, MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_PAIR_12_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_12_END_STABILITY=5.7000
PRIMER_RIGHT_12_END_STABILITY=6.7000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_12=864
PRIMER_PAIR_PENALTY_13=0.0860
PRIMER_LEFT_13_PENALTY=0.057845
PRIMER_RIGHT_13_PENALTY=0.028128
PRIMER_LEFT_13_SEQUENCE=GCTACCAGGTTCCTGTCACATAC
PRIMER_RIGHT_13_SEQUENCE=CAGTAAATCAGCTACCCTGATGG
PRIMER_LEFT_13=2171,23
PRIMER_RIGHT_13=2841,23
PRIMER_LEFT_13_TM=59.942
PRIMER_RIGHT_13_TM=60.028
PRIMER_LEFT_13_GC_PERCENT=52.174
PRIMER_RIGHT_13_GC_PERCENT=47.826
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=6.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=3.00
PRIMER_LEFT_13_MISPRIMING_SCORE=12.00, MER21B a consensus
PRIMER_RIGHT_13_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_13_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_13_END_STABILITY=5.6000
PRIMER_RIGHT_13_END_STABILITY=8.1000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_13=671
PRIMER_PAIR_PENALTY_14=0.0864
PRIMER_LEFT_14_PENALTY=0.045432
PRIMER_RIGHT_14_PENALTY=0.040967
PRIMER_LEFT_14_SEQUENCE=ATGGTCCAGACAGAGGAACAGTA
PRIMER_RIGHT_14_SEQUENCE=ATTGCATATCCTAAAAATCCGGT
PRIMER_LEFT_14=3592,23
PRIMER_RIGHT_14=4179,23
PRIMER_LEFT_14_TM=60.045
PRIMER_RIGHT_14_TM=59.959
PRIMER_LEFT_14_GC_PERCENT=47.826
PRIMER_RIGHT_14_GC_PERCENT=34.783
PRIMER_LEFT_14_SELF_ANY=5.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=2.00
PRIMER_RIGHT_14_SELF_END=2.00
PRIMER_LEFT_14_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_14_MISPRIMING_SCORE=11.00, MER42a MER42 repetitive sequence: subfamily MER42a - a consensus
PRIMER_PAIR_14_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_14_END_STABILITY=5.7000
PRIMER_RIGHT_14_END_STABILITY=11.1000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_14=588
PRIMER_PAIR_PENALTY_15=0.0864
PRIMER_LEFT_15_PENALTY=0.045432
PRIMER_RIGHT_15_PENALTY=0.040967
PRIMER_LEFT_15_SEQUENCE=TATGGTCCAGACAGAGGAACAGT
PRIMER_RIGHT_15_SEQUENCE=ATTGCATATCCTAAAAATCCGGT
PRIMER_LEFT_15=3591,23
PRIMER_RIGHT_15=4179,23
PRIMER_LEFT_15_TM=60.045
PRIMER_RIGHT_15_TM=59.959
PRIMER_LEFT_15_GC_PERCENT=47.826
PRIMER_RIGHT_15_GC_PERCENT=34.783
PRIMER_LEFT_15_SELF_ANY=5.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=3.00
PRIMER_RIGHT_15_SELF_END=2.00
PRIMER_LEFT_15_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_15_MISPRIMING_SCORE=11.00, MER42a MER42 repetitive sequence: subfamily MER42a - a consensus
PRIMER_PAIR_15_MISPRIMING_SCORE=22.00, MER42a MER42 repetitive sequence: subfamily MER42a - a consensus
PRIMER_LEFT_15_END_STABILITY=6.1000
PRIMER_RIGHT_15_END_STABILITY=11.1000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_15=589
PRIMER_PAIR_PENALTY_16=0.0902
PRIMER_LEFT_16_PENALTY=0.010384
PRIMER_RIGHT_16_PENALTY=0.079770
PRIMER_LEFT_16_SEQUENCE=CAGGAATTAGTGCTGGAATTTTG
PRIMER_RIGHT_16_SEQUENCE=TGGAACTGTTTAACTTCACGGAT
PRIMER_LEFT_16=2483,23
PRIMER_RIGHT_16=3353,23
PRIMER_LEFT_16_TM=60.010
PRIMER_RIGHT_16_TM=59.920
PRIMER_LEFT_16_GC_PERCENT=39.130
PRIMER_RIGHT_16_GC_PERCENT=39.130
PRIMER_LEFT_16_SELF_ANY=5.00
PRIMER_RIGHT_16_SELF_ANY=5.00
PRIMER_LEFT_16_SELF_END=2.00
PRIMER_RIGHT_16_SELF_END=2.00
PRIMER_LEFT_16_MISPRIMING_SCORE=11.00, MER4C a consensus
PRIMER_RIGHT_16_MISPRIMING_SCORE=10.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_16_MISPRIMING_SCORE=19.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_16_END_STABILITY=7.6000
PRIMER_RIGHT_16_END_STABILITY=9.8000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_16=871
PRIMER_PAIR_PENALTY_17=0.0902
PRIMER_LEFT_17_PENALTY=0.010384
PRIMER_RIGHT_17_PENALTY=0.079770
PRIMER_LEFT_17_SEQUENCE=CAGGAATTAGTGCTGGAATTTTG
PRIMER_RIGHT_17_SEQUENCE=ATGGAACTGTTTAACTTCACGGA
PRIMER_LEFT_17=2483,23
PRIMER_RIGHT_17=3354,23
PRIMER_LEFT_17_TM=60.010
PRIMER_RIGHT_17_TM=59.920
PRIMER_LEFT_17_GC_PERCENT=39.130
PRIMER_RIGHT_17_GC_PERCENT=39.130
PRIMER_LEFT_17_SELF_ANY=5.00
PRIMER_RIGHT_17_SELF_ANY=5.00
PRIMER_LEFT_17_SELF_END=2.00
PRIMER_RIGHT_17_SELF_END=2.00
PRIMER_LEFT_17_MISPRIMING_SCORE=11.00, MER4C a consensus
PRIMER_RIGHT_17_MISPRIMING_SCORE=11.00, Tigger2 Autonomous DNA transposon
PRIMER_PAIR_17_MISPRIMING_SCORE=20.00, Tigger2 Autonomous DNA transposon
PRIMER_LEFT_17_END_STABILITY=7.6000
PRIMER_RIGHT_17_END_STABILITY=9.6000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_17=872
PRIMER_PAIR_PENALTY_18=0.0924
PRIMER_LEFT_18_PENALTY=0.064245
PRIMER_RIGHT_18_PENALTY=0.028128
PRIMER_LEFT_18_SEQUENCE=TCTCAGCTCCAACTTTACCTGAC
PRIMER_RIGHT_18_SEQUENCE=CAGTAAATCAGCTACCCTGATGG
PRIMER_LEFT_18=1991,23
PRIMER_RIGHT_18=2841,23
PRIMER_LEFT_18_TM=59.936
PRIMER_RIGHT_18_TM=60.028
PRIMER_LEFT_18_GC_PERCENT=47.826
PRIMER_RIGHT_18_GC_PERCENT=47.826
PRIMER_LEFT_18_SELF_ANY=5.00
PRIMER_RIGHT_18_SELF_ANY=6.00
PRIMER_LEFT_18_SELF_END=3.00
PRIMER_RIGHT_18_SELF_END=3.00
PRIMER_LEFT_18_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_18_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_18_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_18_END_STABILITY=6.4000
PRIMER_RIGHT_18_END_STABILITY=8.1000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_18=851
PRIMER_PAIR_PENALTY_19=0.0983
PRIMER_LEFT_19_PENALTY=0.087907
PRIMER_RIGHT_19_PENALTY=0.010384
PRIMER_LEFT_19_SEQUENCE=TCAGATTGCAAGAAGTGACAAAA
PRIMER_RIGHT_19_SEQUENCE=CAGCAATCTTTAATGTCTTTGGG
PRIMER_LEFT_19=983,23
PRIMER_RIGHT_19=1417,23
PRIMER_LEFT_19_TM=59.912
PRIMER_RIGHT_19_TM=60.010
PRIMER_LEFT_19_GC_PERCENT=34.783
PRIMER_RIGHT_19_GC_PERCENT=39.130
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_MISPRIMING_SCORE=12.00, MER34 a consensus
PRIMER_RIGHT_19_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_19_MISPRIMING_SCORE=24.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_19_END_STABILITY=7.6000
PRIMER_RIGHT_19_END_STABILITY=10.0000
PRIMER_PAIR_19_COMPL_ANY=7.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_19=435
=