PRIMER_SEQUENCE_ID=NM_002846
SEQUENCE=GCGGCAAGCCCCGCCTCAGCCCCTCTGGCAGGCTCCCGCCAGCGTCGCTGCGGCTCCGGCCCGGGAGCGAGCGCCCGGAGCTCGGAAAGATGCGGCGCCCGCGGCGGCCTGGGGGTCTCGGGGGATCCGGGGGTCTCCGGCTGCTCCTCTGCCTCCTGCTGCTGAGCAGCCGCCCGGGGGGCTGCAGCGCCGTTAGTGCCCACGGCTGTCTATTTGACCGCAGGCTCTGCTCTCACCTGGAAGTCTGTATTCAGGATGGCTTGTTTGGGCAGTGCCAGGTGGGAGTGGGGCAGGCCCGGCCCCTTTTGCAAGTCACCTCCCCAGTTCTCCAACGCTTACAAGGTGTGCTCCGACAACTCATGTCCCAAGGATTGTCCTGGCACGATGACCTCACCCAGTATGTGATCTCTCAGGAGATGGAGCGCATCCCCAGGCTTCGCCCCCCAGAGCCCCGTCCAAGGGACAGGTCTGGCTTGGCACCCAAGAGACCTGGTCCTGCTGGAGAGCTGCTTTTACAGGACATCCCCACTGGCTCCGCCCCTGCTGCCCAGCATCGGCTTCCACAACCACCAGTGGGCAAAGGTGGAGCTGGGGCCAGCTCCTCTCTGTCCCCTCTGCAGGCTGAGCTGCTCCCGCCTCTCTTGGAGCACCTGCTGCTGCCCCCACAGCCTCCCCACCCTTCACTGAGTTACGAACCTGCCTTGCTGCAGCCCTACCTGTTCCACCAGTTTGGCTCCCGTGATGGCTCCAGGGTCTCAGAGGGCTCCCCAGGGATGGTCAGTGTCGGCCCCCTGCCCAAGGCTGAAGCCCCTGCCCTCTTCAGCAGAACTGCCTCCAAGGGCATATTTGGGGACCACCCTGGCCACTCCTACGGGGACCTTCCAGGGCCTTCACCTGCCCAGCTTTTTCAAGACTCTGGGCTGCTCTATCTGGCCCAGGAGTTGCCAGCACCCAGCAGGGCCAGGGTGCCAAGGCTGCCAGAGCAAGGGAGCAGCAGCCGGGCAGAGGACTCCCCAGAGGGCTATGAGAAGGAAGGACTAGGGGATCGTGGAGAGAAGCCTGCTTCCCCAGCTGTGCAGCCAGATGCGGCTCTGCAGAGGCTGGCCGCTGTGCTGGCGGGCTATGGGGTAGAGCTGCGTCAGCTGACCCCTGAGCAGCTCTCCACACTCCTGACCCTGCTGCAGCTACTGCCCAAGGGTGCAGGAAGAAATCCGGGAGGGGTTGTAAATGTTGGAGCTGATATCAAGAAAACAATGGAGGGGCCGGTGGAGGGCAGAGACACAGCAGAGCTTCCAGCCCGCACATCCCCCATGCCTGGACACCCCACTGCCAGCCCTACCTCCAGTGAAGTCCAGCAGGTGCCAAGCCCTGTCTCCTCTGAGCCTCCCAAAGCTGCCAGACCCCCTGTGACACCTGTCCTGCTAGAGAAGAAAAGCCCACTGGGCCAGAGCCAGCCCACGGTGGCAGGACAGCCCTCAGCCCGCCCAGCAGCAGAGGAATATGGCTACATCGTCACTGATCAGAAGCCCCTGAGCCTGGCTGCAGGAGTGAAGCTGCTGGAGATCCTGGCTGAGCATGTGCACATGTCCTCAGGCAGCTTCATCAACATCAGTGTGGTGGGACCAGCCCTCACCTTCCGCATCCGGCACAATGAGCAGAACCTGTCTTTGGCTGATGTGACCCAACAAGCAGGGCTGGTGAAGTCTGAACTGGAAGCACAGACAGGGCTCCAAATCTTGCAGACAGGAGTGGGACAGAGGGAGGAGGCAGCTGCAGTCCTTCCCCAAACTGCGCACAGCACCTCACCCATGCGCTCAGTGCTGCTCACTCTGGTGGCCCTGGCAGGTGTGGCTGGGCTGCTGGTGGCTCTGGCTGTGGCTCTGTGTGTGCGGCAGCATGCGCGGCAGCAAGACAAGGAGCGCCTGGCAGCCCTGGGGCCTGAGGGGGCCCATGGTGACACTACCTTTGAGTACCAGGACCTGTGCCGCCAGCACATGGCCACGAAGTCCTTGTTCAACCGGGCAGAGGGTCCACCGGAGCCTTCACGGGTGAGCAGTGTGTCCTCCCAGTTCAGCGACGCAGCCCAGGCCAGCCCCAGCTCCCACAGCAGCACCCCGTCCTGGTGCGAGGAGCCGGCCCAAGCCAACATGGACATCTCCACGGGACACATGATTCTGGCATACATGGAGGATCACCTGCGGAACCGGGACCGCCTTGCCAAGGAGTGGCAGGCCCTCTGTGCCTACCAAGCAGAGCCAAACACCTGTGCCACCGCGCAGGGGGAGGGCAACATCAAAAAGAACCGGCATCCTGACTTCCTGCCCTATGACCATGCCCGCATAAAACTGAAGGTGGAGAGCAGCCCTTCTCGGAGCGATTACATCAACGCCAGCCCCATTATTGAGCATGACCCTCGGATGCCAGCCTACATAGCCACGCAGGGCCCGCTGTCCCATACCATCGCAGACTTCTGGCAGATGGTGTGGGAGAGCGGCTGCACCGTCATCGTCATGCTGACCCCGCTGGTGGAGGATGGTGTCAAGCAGTGTGACCGCTACTGGCCAGATGAGGGTGCCTCCCTCTACCACGTATATGAGGTGAACCTGGTGTCGGAGCACATCTGGTGCGAGGACTTTCTGGTGCGGAGCTTCTACCTGAAGAACGTGCAGACCCAGGAGACGCGCACGCTCACGCAGTTCCACTTCCTCAGCTGGCCGGCAGAGGGCACACCGGCCTCCACGCGGCCCCTGCTGGACTTCCGCAGGAAGGTGAACAAGTGCTACCGGGGCCGCTCCTGCCCCATCATCGTGCACTGCAGTGATGGTGCGGGGAGGACCGGCACCTACATCCTCATCGACATGGTCCTGAACCGCATGGCAAAAGGAGTGAAGGAGATTGACATCGCTGCCACCCTGGAGCATGTCCGTGACCAGCGGCCTGGCCTTGTCCGCTCTAAGGACCAGTTTGAATTTGCCCTGACAGCCGTGGCGGAGGAAGTGAATGCCATCCTCAAGGCCCTGCCCCAGTGAGACCCTGGGGCCCCTTGGCGGGCAGCCCAGCCTCTGTCCCTCTTTGCCTGTGTGAGCATCTCTGTGTACCCACTCCTCACTGCCCCACCAGCCACCTCTTGGGCATGCTCAGCCCTTCCTAGAAGAGTCAGGAAGGGAAAGCCAGAAGGGGCACGCCTGCCCAGCCTCGCATGCCAGAGCCTGGGGCATCCCAGAGCCCAGGGCATCCCATGGGGGTGCTGCAGCCAGGAGGAGAGGAAAGGACATGGGTAGCAATTCTACCCAGAGCCTTCTCCTGCCTACATTCCCTGGCCTGGCTCTCCTGTAGCTCTCCTGGGGTTCTGGGAGTTCCCTGAACATCTGTGTGTGTCCCCCTATGCTCCAGTATGGAAGAATGGGGTGGAGGGTCGCCACACCCGGCTCCCCCTGCTTCTCAGCCCCGGGCCTGCCTCTGACTCACACTTGGGCGCTCTGCCCTCCCTGGCCTCACGCCCAGCCTCCTCCCACCACCCTCCCACCATGCGCTGCTCAACCTCTCTCCTTCTGGCGCAAGAGAACATTTCTAGAAAAAACTACTTTTGTACCAGTGTGAATAAAGTTAGTGTGTTGTCTGTGCAGCTGCAAAAAAAAAAAAAAAAAAA
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 42419, GC content failed 1598, low tm 7853, high tm 25562, high any compl 1, high end compl 31, high repeat similarity 26, long poly-x seq 34, ok 7314
PRIMER_RIGHT_EXPLAIN=considered 42146, GC content failed 1250, low tm 8302, high tm 25121, high any compl 2, high end compl 14, high repeat similarity 8, long poly-x seq 70, ok 7379
PRIMER_PAIR_EXPLAIN=considered 1500, unacceptable product size 1417, high any compl 2, high end compl 15, ok 66
PRIMER_PAIR_PENALTY=0.0612
PRIMER_LEFT_PENALTY=0.061028
PRIMER_RIGHT_PENALTY=0.000148
PRIMER_LEFT_SEQUENCE=CACCTGTCCTGCTAGAGAAGAAA
PRIMER_RIGHT_SEQUENCE=GACGATGTAGCCATATTCCTCTG
PRIMER_LEFT=1410,23
PRIMER_RIGHT=1513,23
PRIMER_LEFT_TM=60.061
PRIMER_RIGHT_TM=60.000
PRIMER_LEFT_GC_PERCENT=47.826
PRIMER_RIGHT_GC_PERCENT=47.826
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_MISPRIMING_SCORE=12.00, reverse LTR4 LTR from human endogenous retrovirus ERV3, POL-ENV-3'LTR region
PRIMER_RIGHT_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=104
PRIMER_PAIR_PENALTY_1=0.1904
PRIMER_LEFT_1_PENALTY=0.061028
PRIMER_RIGHT_1_PENALTY=0.129348
PRIMER_LEFT_1_SEQUENCE=CACCTGTCCTGCTAGAGAAGAAA
PRIMER_RIGHT_1_SEQUENCE=GATGTAGCCATATTCCTCTGCTG
PRIMER_LEFT_1=1410,23
PRIMER_RIGHT_1=1510,23
PRIMER_LEFT_1_TM=60.061
PRIMER_RIGHT_1_TM=60.129
PRIMER_LEFT_1_GC_PERCENT=47.826
PRIMER_RIGHT_1_GC_PERCENT=47.826
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_MISPRIMING_SCORE=12.00, reverse LTR4 LTR from human endogenous retrovirus ERV3, POL-ENV-3'LTR region
PRIMER_RIGHT_1_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_1_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=101
PRIMER_PAIR_PENALTY_2=0.2309
PRIMER_LEFT_2_PENALTY=0.230707
PRIMER_RIGHT_2_PENALTY=0.000148
PRIMER_LEFT_2_SEQUENCE=AGGGCTATGAGAAGGAAGGACTA
PRIMER_RIGHT_2_SEQUENCE=GACGATGTAGCCATATTCCTCTG
PRIMER_LEFT_2=1017,23
PRIMER_RIGHT_2=1513,23
PRIMER_LEFT_2_TM=59.769
PRIMER_RIGHT_2_TM=60.000
PRIMER_LEFT_2_GC_PERCENT=47.826
PRIMER_RIGHT_2_GC_PERCENT=47.826
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_2_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_2_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_2_END_STABILITY=5.4000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=497
PRIMER_PAIR_PENALTY_3=0.2309
PRIMER_LEFT_3_PENALTY=0.230707
PRIMER_RIGHT_3_PENALTY=0.000148
PRIMER_LEFT_3_SEQUENCE=GGCTATGAGAAGGAAGGACTAGG
PRIMER_RIGHT_3_SEQUENCE=GACGATGTAGCCATATTCCTCTG
PRIMER_LEFT_3=1019,23
PRIMER_RIGHT_3=1513,23
PRIMER_LEFT_3_TM=59.769
PRIMER_RIGHT_3_TM=60.000
PRIMER_LEFT_3_GC_PERCENT=52.174
PRIMER_RIGHT_3_GC_PERCENT=47.826
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_3_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_3_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=495
PRIMER_PAIR_PENALTY_4=0.2357
PRIMER_LEFT_4_PENALTY=0.004943
PRIMER_RIGHT_4_PENALTY=0.230707
PRIMER_LEFT_4_SEQUENCE=CTGGAAGTCTGTATTCAGGATGG
PRIMER_RIGHT_4_SEQUENCE=CTAGTCCTTCCTTCTCATAGCCC
PRIMER_LEFT_4=236,23
PRIMER_RIGHT_4=1040,23
PRIMER_LEFT_4_TM=60.005
PRIMER_RIGHT_4_TM=59.769
PRIMER_LEFT_4_GC_PERCENT=47.826
PRIMER_RIGHT_4_GC_PERCENT=52.174
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_MISPRIMING_SCORE=11.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_4_MISPRIMING_SCORE=11.00, L1PA7 3'-end of L1 repeat (subfamily L1PA7) - a consensus sequence
PRIMER_PAIR_4_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=10.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=805
PRIMER_PAIR_PENALTY_5=0.2357
PRIMER_LEFT_5_PENALTY=0.004943
PRIMER_RIGHT_5_PENALTY=0.230707
PRIMER_LEFT_5_SEQUENCE=CCTGGAAGTCTGTATTCAGGATG
PRIMER_RIGHT_5_SEQUENCE=CTAGTCCTTCCTTCTCATAGCCC
PRIMER_LEFT_5=235,23
PRIMER_RIGHT_5=1040,23
PRIMER_LEFT_5_TM=60.005
PRIMER_RIGHT_5_TM=59.769
PRIMER_LEFT_5_GC_PERCENT=47.826
PRIMER_RIGHT_5_GC_PERCENT=52.174
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=2.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_5_MISPRIMING_SCORE=11.00, L1PA7 3'-end of L1 repeat (subfamily L1PA7) - a consensus sequence
PRIMER_PAIR_5_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_5_END_STABILITY=8.1000
PRIMER_RIGHT_5_END_STABILITY=10.9000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_5=806
PRIMER_PAIR_PENALTY_6=0.2357
PRIMER_LEFT_6_PENALTY=0.004943
PRIMER_RIGHT_6_PENALTY=0.230707
PRIMER_LEFT_6_SEQUENCE=CTGGAAGTCTGTATTCAGGATGG
PRIMER_RIGHT_6_SEQUENCE=TAGTCCTTCCTTCTCATAGCCCT
PRIMER_LEFT_6=236,23
PRIMER_RIGHT_6=1039,23
PRIMER_LEFT_6_TM=60.005
PRIMER_RIGHT_6_TM=59.769
PRIMER_LEFT_6_GC_PERCENT=47.826
PRIMER_RIGHT_6_GC_PERCENT=47.826
PRIMER_LEFT_6_SELF_ANY=6.00
PRIMER_RIGHT_6_SELF_ANY=2.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=2.00
PRIMER_LEFT_6_MISPRIMING_SCORE=11.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_6_MISPRIMING_SCORE=12.00, L1MC2 3'-end of L1 repeat (subfamily L1MC2) - a consensus sequence
PRIMER_PAIR_6_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_6_END_STABILITY=8.1000
PRIMER_RIGHT_6_END_STABILITY=10.9000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_6=804
PRIMER_PAIR_PENALTY_7=0.2357
PRIMER_LEFT_7_PENALTY=0.004943
PRIMER_RIGHT_7_PENALTY=0.230707
PRIMER_LEFT_7_SEQUENCE=CTGGAAGTCTGTATTCAGGATGG
PRIMER_RIGHT_7_SEQUENCE=CCTAGTCCTTCCTTCTCATAGCC
PRIMER_LEFT_7=236,23
PRIMER_RIGHT_7=1041,23
PRIMER_LEFT_7_TM=60.005
PRIMER_RIGHT_7_TM=59.769
PRIMER_LEFT_7_GC_PERCENT=47.826
PRIMER_RIGHT_7_GC_PERCENT=52.174
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_MISPRIMING_SCORE=11.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_7_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_7_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_7_END_STABILITY=8.1000
PRIMER_RIGHT_7_END_STABILITY=8.7000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_7=806
PRIMER_PAIR_PENALTY_8=0.2357
PRIMER_LEFT_8_PENALTY=0.004943
PRIMER_RIGHT_8_PENALTY=0.230707
PRIMER_LEFT_8_SEQUENCE=CTGGAAGTCTGTATTCAGGATGG
PRIMER_RIGHT_8_SEQUENCE=CCCTAGTCCTTCCTTCTCATAGC
PRIMER_LEFT_8=236,23
PRIMER_RIGHT_8=1042,23
PRIMER_LEFT_8_TM=60.005
PRIMER_RIGHT_8_TM=59.769
PRIMER_LEFT_8_GC_PERCENT=47.826
PRIMER_RIGHT_8_GC_PERCENT=52.174
PRIMER_LEFT_8_SELF_ANY=6.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=2.00
PRIMER_LEFT_8_MISPRIMING_SCORE=11.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_8_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_8_END_STABILITY=8.1000
PRIMER_RIGHT_8_END_STABILITY=7.1000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_8=807
PRIMER_PAIR_PENALTY_9=0.2357
PRIMER_LEFT_9_PENALTY=0.004943
PRIMER_RIGHT_9_PENALTY=0.230707
PRIMER_LEFT_9_SEQUENCE=CCTGGAAGTCTGTATTCAGGATG
PRIMER_RIGHT_9_SEQUENCE=TAGTCCTTCCTTCTCATAGCCCT
PRIMER_LEFT_9=235,23
PRIMER_RIGHT_9=1039,23
PRIMER_LEFT_9_TM=60.005
PRIMER_RIGHT_9_TM=59.769
PRIMER_LEFT_9_GC_PERCENT=47.826
PRIMER_RIGHT_9_GC_PERCENT=47.826
PRIMER_LEFT_9_SELF_ANY=6.00
PRIMER_RIGHT_9_SELF_ANY=2.00
PRIMER_LEFT_9_SELF_END=2.00
PRIMER_RIGHT_9_SELF_END=2.00
PRIMER_LEFT_9_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, L1MC2 3'-end of L1 repeat (subfamily L1MC2) - a consensus sequence
PRIMER_PAIR_9_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_9_END_STABILITY=8.1000
PRIMER_RIGHT_9_END_STABILITY=10.9000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_9=805
PRIMER_PAIR_PENALTY_10=0.2357
PRIMER_LEFT_10_PENALTY=0.004943
PRIMER_RIGHT_10_PENALTY=0.230707
PRIMER_LEFT_10_SEQUENCE=CCTGGAAGTCTGTATTCAGGATG
PRIMER_RIGHT_10_SEQUENCE=CCTAGTCCTTCCTTCTCATAGCC
PRIMER_LEFT_10=235,23
PRIMER_RIGHT_10=1041,23
PRIMER_LEFT_10_TM=60.005
PRIMER_RIGHT_10_TM=59.769
PRIMER_LEFT_10_GC_PERCENT=47.826
PRIMER_RIGHT_10_GC_PERCENT=52.174
PRIMER_LEFT_10_SELF_ANY=6.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=2.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_10_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_10_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_10_END_STABILITY=8.1000
PRIMER_RIGHT_10_END_STABILITY=8.7000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_10=807
PRIMER_PAIR_PENALTY_11=0.2357
PRIMER_LEFT_11_PENALTY=0.004943
PRIMER_RIGHT_11_PENALTY=0.230707
PRIMER_LEFT_11_SEQUENCE=CCTGGAAGTCTGTATTCAGGATG
PRIMER_RIGHT_11_SEQUENCE=CCCTAGTCCTTCCTTCTCATAGC
PRIMER_LEFT_11=235,23
PRIMER_RIGHT_11=1042,23
PRIMER_LEFT_11_TM=60.005
PRIMER_RIGHT_11_TM=59.769
PRIMER_LEFT_11_GC_PERCENT=47.826
PRIMER_RIGHT_11_GC_PERCENT=52.174
PRIMER_LEFT_11_SELF_ANY=6.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=2.00
PRIMER_RIGHT_11_SELF_END=2.00
PRIMER_LEFT_11_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_11_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_11_END_STABILITY=8.1000
PRIMER_RIGHT_11_END_STABILITY=7.1000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_11=808
PRIMER_PAIR_PENALTY_12=0.2449
PRIMER_LEFT_12_PENALTY=0.014220
PRIMER_RIGHT_12_PENALTY=0.230707
PRIMER_LEFT_12_SEQUENCE=TCTGTATTCAGGATGGCTTGTTT
PRIMER_RIGHT_12_SEQUENCE=CTAGTCCTTCCTTCTCATAGCCC
PRIMER_LEFT_12=243,23
PRIMER_RIGHT_12=1040,23
PRIMER_LEFT_12_TM=60.014
PRIMER_RIGHT_12_TM=59.769
PRIMER_LEFT_12_GC_PERCENT=39.130
PRIMER_RIGHT_12_GC_PERCENT=52.174
PRIMER_LEFT_12_SELF_ANY=6.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_MISPRIMING_SCORE=11.00, MER8 Nonautonomous DNA transposon
PRIMER_RIGHT_12_MISPRIMING_SCORE=11.00, L1PA7 3'-end of L1 repeat (subfamily L1PA7) - a consensus sequence
PRIMER_PAIR_12_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_12_END_STABILITY=7.0000
PRIMER_RIGHT_12_END_STABILITY=10.9000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_12=798
PRIMER_PAIR_PENALTY_13=0.2449
PRIMER_LEFT_13_PENALTY=0.014220
PRIMER_RIGHT_13_PENALTY=0.230707
PRIMER_LEFT_13_SEQUENCE=TCTGTATTCAGGATGGCTTGTTT
PRIMER_RIGHT_13_SEQUENCE=TAGTCCTTCCTTCTCATAGCCCT
PRIMER_LEFT_13=243,23
PRIMER_RIGHT_13=1039,23
PRIMER_LEFT_13_TM=60.014
PRIMER_RIGHT_13_TM=59.769
PRIMER_LEFT_13_GC_PERCENT=39.130
PRIMER_RIGHT_13_GC_PERCENT=47.826
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=2.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=2.00
PRIMER_LEFT_13_MISPRIMING_SCORE=11.00, MER8 Nonautonomous DNA transposon
PRIMER_RIGHT_13_MISPRIMING_SCORE=12.00, L1MC2 3'-end of L1 repeat (subfamily L1MC2) - a consensus sequence
PRIMER_PAIR_13_MISPRIMING_SCORE=21.00, MER39 a consensus
PRIMER_LEFT_13_END_STABILITY=7.0000
PRIMER_RIGHT_13_END_STABILITY=10.9000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_13=797
PRIMER_PAIR_PENALTY_14=0.2449
PRIMER_LEFT_14_PENALTY=0.014220
PRIMER_RIGHT_14_PENALTY=0.230707
PRIMER_LEFT_14_SEQUENCE=TCTGTATTCAGGATGGCTTGTTT
PRIMER_RIGHT_14_SEQUENCE=CCTAGTCCTTCCTTCTCATAGCC
PRIMER_LEFT_14=243,23
PRIMER_RIGHT_14=1041,23
PRIMER_LEFT_14_TM=60.014
PRIMER_RIGHT_14_TM=59.769
PRIMER_LEFT_14_GC_PERCENT=39.130
PRIMER_RIGHT_14_GC_PERCENT=52.174
PRIMER_LEFT_14_SELF_ANY=6.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_MISPRIMING_SCORE=11.00, MER8 Nonautonomous DNA transposon
PRIMER_RIGHT_14_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_14_MISPRIMING_SCORE=20.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_14_END_STABILITY=7.0000
PRIMER_RIGHT_14_END_STABILITY=8.7000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_14=799
PRIMER_PAIR_PENALTY_15=0.2449
PRIMER_LEFT_15_PENALTY=0.014220
PRIMER_RIGHT_15_PENALTY=0.230707
PRIMER_LEFT_15_SEQUENCE=TCTGTATTCAGGATGGCTTGTTT
PRIMER_RIGHT_15_SEQUENCE=CCCTAGTCCTTCCTTCTCATAGC
PRIMER_LEFT_15=243,23
PRIMER_RIGHT_15=1042,23
PRIMER_LEFT_15_TM=60.014
PRIMER_RIGHT_15_TM=59.769
PRIMER_LEFT_15_GC_PERCENT=39.130
PRIMER_RIGHT_15_GC_PERCENT=52.174
PRIMER_LEFT_15_SELF_ANY=6.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=2.00
PRIMER_LEFT_15_MISPRIMING_SCORE=11.00, MER8 Nonautonomous DNA transposon
PRIMER_RIGHT_15_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_15_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_15_END_STABILITY=7.0000
PRIMER_RIGHT_15_END_STABILITY=7.1000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_15=800
PRIMER_PAIR_PENALTY_16=0.3613
PRIMER_LEFT_16_PENALTY=0.361198
PRIMER_RIGHT_16_PENALTY=0.000148
PRIMER_LEFT_16_SEQUENCE=CTATGAGAAGGAAGGACTAGGGG
PRIMER_RIGHT_16_SEQUENCE=GACGATGTAGCCATATTCCTCTG
PRIMER_LEFT_16=1021,23
PRIMER_RIGHT_16=1513,23
PRIMER_LEFT_16_TM=59.639
PRIMER_RIGHT_16_TM=60.000
PRIMER_LEFT_16_GC_PERCENT=52.174
PRIMER_RIGHT_16_GC_PERCENT=47.826
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_16_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_16_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_16_END_STABILITY=10.9000
PRIMER_RIGHT_16_END_STABILITY=6.7000
PRIMER_PAIR_16_COMPL_ANY=6.00
PRIMER_PAIR_16_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_16=493
PRIMER_PAIR_PENALTY_17=0.3661
PRIMER_LEFT_17_PENALTY=0.004943
PRIMER_RIGHT_17_PENALTY=0.361198
PRIMER_LEFT_17_SEQUENCE=CTGGAAGTCTGTATTCAGGATGG
PRIMER_RIGHT_17_SEQUENCE=CCCCTAGTCCTTCCTTCTCATAG
PRIMER_LEFT_17=236,23
PRIMER_RIGHT_17=1043,23
PRIMER_LEFT_17_TM=60.005
PRIMER_RIGHT_17_TM=59.639
PRIMER_LEFT_17_GC_PERCENT=47.826
PRIMER_RIGHT_17_GC_PERCENT=52.174
PRIMER_LEFT_17_SELF_ANY=6.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=2.00
PRIMER_LEFT_17_MISPRIMING_SCORE=11.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_17_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_17_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_17_END_STABILITY=8.1000
PRIMER_RIGHT_17_END_STABILITY=5.9000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_17=808
PRIMER_PAIR_PENALTY_18=0.3661
PRIMER_LEFT_18_PENALTY=0.004943
PRIMER_RIGHT_18_PENALTY=0.361198
PRIMER_LEFT_18_SEQUENCE=CCTGGAAGTCTGTATTCAGGATG
PRIMER_RIGHT_18_SEQUENCE=CCCCTAGTCCTTCCTTCTCATAG
PRIMER_LEFT_18=235,23
PRIMER_RIGHT_18=1043,23
PRIMER_LEFT_18_TM=60.005
PRIMER_RIGHT_18_TM=59.639
PRIMER_LEFT_18_GC_PERCENT=47.826
PRIMER_RIGHT_18_GC_PERCENT=52.174
PRIMER_LEFT_18_SELF_ANY=6.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=2.00
PRIMER_RIGHT_18_SELF_END=2.00
PRIMER_LEFT_18_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_18_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_18_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_18_END_STABILITY=8.1000
PRIMER_RIGHT_18_END_STABILITY=5.9000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_18=809
PRIMER_PAIR_PENALTY_19=0.3754
PRIMER_LEFT_19_PENALTY=0.014220
PRIMER_RIGHT_19_PENALTY=0.361198
PRIMER_LEFT_19_SEQUENCE=TCTGTATTCAGGATGGCTTGTTT
PRIMER_RIGHT_19_SEQUENCE=CCCCTAGTCCTTCCTTCTCATAG
PRIMER_LEFT_19=243,23
PRIMER_RIGHT_19=1043,23
PRIMER_LEFT_19_TM=60.014
PRIMER_RIGHT_19_TM=59.639
PRIMER_LEFT_19_GC_PERCENT=39.130
PRIMER_RIGHT_19_GC_PERCENT=52.174
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=2.00
PRIMER_LEFT_19_MISPRIMING_SCORE=11.00, MER8 Nonautonomous DNA transposon
PRIMER_RIGHT_19_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_19_MISPRIMING_SCORE=20.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_19_END_STABILITY=7.0000
PRIMER_RIGHT_19_END_STABILITY=5.9000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_19=801
=