PRIMER_SEQUENCE_ID=NM_002850
SEQUENCE=ATTCCATCCCATCACCCCATGATCCTTCCCCATTGTCCCATGAACAGCCCCTGCAGGACCCTCCCCTGCTCCATGTGACCCTCCCTCTGCCTCCTCCATGAAGGTCTCCTGGATTTCCCCACTCGCCTCTCACAGCCCTCCATTGTCTCTGCAGACGTTGCCCCATCTGACGCTCGGCTCGAGGCCTCTCTGTGAGGGACCGGGGGGCCATCCCCCTCCAGGGCGGAGATCGGAGGTCGCTGCCAAGCATGGCGCCCACCTGGGGCCCTGGCATGGTGTCTGTGGTTGGTCCCATGGGCCTCCTTGTGGTCCTGCTCGTTGGAGGCTGTGCAGCAGAAGAGCCCCCCAGGTTTATCAAAGAACCCAAGGACCAGATCGGCGTGTCGGGGGGTGTGGCCTCTTTCGTGTGTCAGGCCACGGGTGACCCCAAGCCACGAGTGACCTGGAACAAGAAGGGCAAGAAGGTCAACTCTCAGCGCTTTGAGACGATTGAGTTTGATGAGAGTGCAGGGGCAGTGCTGAGGATCCAGCCGCTGAGGACACCGCGGGATGAAAACGTGTACGAGTGTGTGGCCCAGAACTCGGTTGGGGAGATCACAGTCCATGCCAAGCTTACTGTCCTCCGAGAGGACCAGCTGCCCTCTGGCTTCCCCAACATCGACATGGGCCCACAGTTGAAGGTGGTGGAGCGGACACGGACAGCCACCATGCTCTGTGCAGCCAGCGGCAACCCTGACCCTGAGATCACCTGGTTCAAGGACTTCCTGCCTGTGGATCCTAGTGCCAGCAATGGACGCATCAAACAGCTGCGATCAGAAACCTTTGAAAGCACTCCGATTCGAGGAGCCCTGCAGATTGAAAGCAGTGAGGAAACCGACCAGGGCAAATATGAGTGTGTGGCCACCAACAGCGCCGGCGTGCGCTACTCCTCACCTGCCAACCTCTACGTGCGAGAGCTTCGAGAAGTCCGCCGCGTGGCCCCGCGCTTCTCCATCCTGCCCATGAGCCACGAGATCATGCCAGGGGGCAACGTGAACATCACCTGCGTGGCCGTGGGCTCGCCCATGCCATACGTGAAGTGGATGCAGGGGGCCGAGGACCTGACCCCCGAGGATGACATGCCCGTGGGTCGGAACGTGCTGGAACTCACAGATGTCAAGGACTCGGCCAACTACACCTGCGTGGCCATGTCCAGCCTGGGCGTCATTGAGGCGGTTGCTCAGATCACGGTGAAATCTCTCCCCAAAGCTCCCGGGACTCCCATGGTGACTGAGAACACAGCCACCAGCATCACCATCACGTGGGACTCGGGCAACCCAGATCCTGTGTCCTATTACGTCATCGAATATAAATCCAAGAGCCAAGACGGGCCGTATCAGATTAAAGAGGACATCACCACCACACGTTACAGCATCGGCGGCCTGAGCCCCAACTCGGAGTACGAGATCTGGGTGTCGGCCGTCAACTCCATCGGCCAGGGGCCCCCCAGCGAGTCCGTGGTCACCCGCACAGGCGAGCAGGCCCCGGCCAGCGCGCCGCGGAACGTGCAAGCCCGGATGCTCAGCGCGACCACCATGATTGTGCAGTGGGAGGAGCCGGTGGAGCCCAACGGCCTGATCCGCGGCTACCGCGTCTACTACACCATGGAACCGGAGCACCCCGTGGGCAACTGGCAGAAGCACAACGTGGACGACAGCCTGCTGACCACCGTGGGCAGCCTGCTGGAGGACGAGACCTACACCGTGCGGGTGCTCGCCTTCACCTCCGTCGGCGACGGGCCCCTCTCGGACCCCATCCAGGTCAAGACGCAGCAGGGAGTGCCGGGCCAGCCCATGAACCTGCGGGCCGAGGCCAGGTCGGAGACCAGCATCACGCTGTCCTGGAGCCCCCCGCGGCAGGAGAGTATCATCAAGTACGAGCTCCTCTTCCGGGAAGGCGACCATGGCCGGGAGGTGGGAAGGACCTTCGACCCGACGACTTCCTACGTGGTGGAGGACCTGAAGCCCAACACGGAGTACGCCTTCCGCCTGGCGGCCCGCTCGCCGCAGGGCCTGGGCGCCTTCACCCCCGTGGTGCGGCAGCGCACGCTGCAGTCCAAACCGTCAGCCCCCCCTCAAGACGTTAAATGTGTCAGCGTGCGCTCCACGGCCATTTTGGTAAGTTGGCGCCCGCCGCCGCCGGAAACGCACAACGGGGCCCTGGTGGGCTACAGCGTCCGCTACCGACCGCTGGGCTCAGAGGACCCGGAACCCAAGGAGGTGAACGGCATCCCCCCGACCACCACTCAGATCCTGCTGGAGGCCTTGGAGAAGTGGACCCAGTACCGCATCACGACTGTCGCTCACACAGAGGTGGGACCAGGGCCCGAGAGCTCGCCCGTGGTCGTCCGCACCGACGAGGATGTGCCCAGCGCGCCGCCGCGGAAGGTGGAGGCGGAGGCGCTCAACGCCACGGCCATCCGCGTGCTGTGGCGCTCGCCCGCGCCCGGCCGGCAGCACGGCCAGATCCGCGGCTACCAGGTCCACTACGTGCGCATGGAGGGCGCCGAGGCCCGCGGGCCGCCGCGCATCAAGGACGTCATGCTGGCCGATGCCCAGTGGGAGACGGATGACACGGCCGAATATGAGATGGTCATCACAAACTTGCAGCCTGAGACCGCGTACTCCATCACGGTAGCCGCCTACACCATGAAGGGCGATGGCGCTCGCAGCAAACCCAAGGTGGTTGTGACCAAGGGAGCAGTGCTGGGCCGCCCAACCCTGTCGGTGCAGCAGACCCCCGAGGGCAGCCTGCTGGCACGCTGGGAGCCCCCGGCTGGCACCGCGGAGGACCAGGTGCTGGGCTACCGCCTGCAGTTTGGCCGTGAGGACTCGACGCCCCTGGCCACCCTGGAGTTCCCGCCCTCCGAGGACCGCTACACGGCATCAGGCGTGCACAAGGGGGCCACGTATGTGTTCCGGCTTGCGGCCCGGAGCCGCGGCGGCCTGGGCGAGGAGGCAGCCGAGGTCCTGAGCATCCCGGAGGACACGCCCCGTGGCCACCCGCAGATTCTGGAGGCGGCCGGCAACGCCTCGGCCGGGACCGTCCTTCTCCGCTGGCTGCCACCCGTGCCCGCCGAGCGCAACGGGGCCATCGTCAAATACACGGTGGCCGTGCGGGAGGCCGGTGCCCTGGGCCCTGCCCGAGAGACTGAGCTGCCGGCAGCGGCTGAGCCGGGCGCGGAGAACGCGCTCACGCTGCAGGGCCTGAAGCCCGACACGGCCTATGACCTCCAAGTGCGAGCCCACACGCGCCGGGGCCCTGGCCCCTTCAGCCCCCCCGTCCGCTACCGGACGTTCCTGCGGGACCAAGTCTCGCCCAAGAACTTCAAGGTGAAAATGATCATGAAGACATCAGTTCTGCTCAGCTGGGAGTTCCCTGACAACTACAACTCACCCACACCCTACAAGATCCAGTACAATGGGCTCACACTGGATGTGGATGGCCGTACCACCAAGAAGCTCATCACGCACCTCAAGCCCCACACCTTCTACAACTTTGTGCTGACCAATCGCGGCAGCAGCCTGGGCGGCCTCCAGCAGACGGTCACCGCCTGGACTGCCTTCAACCTGCTCAACGGCAAGCCCAGCGTCGCCCCCAAGCCTGATGCTGACGGCTTCATCATGGTGTATCTTCCTGACGGCCAGAGCCCCGTGCCTGTCCAGAGCTATTTCATTGTGATGGTGCCACTGCGCAAGTCTCGTGGAGGCCAATTCCTGACCCCGCTGGGTAGCCCAGAGGACATGGATCTGGAAGAGCTCATCCAGGACATCTCACGGCTACAGAGGCGCAGCCTGCGGCACTCGCGTCAGCTGGAGGTGCCCCGGCCCTATATTGCAGCTCGCTTCTCTGTGCTGCCACCCACGTTCCATCCCGGCGACCAGAAGCAGTATGGCGGCTTCGATAACCGGGGCCTGGAGCCCGGCCACCGCTATGTCCTCTTCGTGCTTGCCGTGCTTCAGAAGAGCGAGCCTACCTTTGCAGCCAGTCCCTTCTCAGACCCCTTCCAGCTGGATAACCCGGACCCCCAGCCCATCGTGGATGGCGAGGAGGGGCTTATCTGGGTGATCGGGCCTGTGCTGGCCGTGGTCTTCATAATCTGCATTGTCATTGCTATCCTGCTCTACAAGAACAAACCCGACAGTAAACGCAAGGACTCAGAACCCCGCACCAAATGCCTCCTGAACAATGCCGACCTCGCCCCTCACCACCCCAAGGACCCTGTGGAAATGAGACGCATTAACTTCCAGACTCCAGATTCAGGCCTCAGGAGCCCCCTCAGGGAGCCGGGGTTTCACTTTGAAAGCATGCTTAGCCACCCGCCAATTCCCATCGCAGACATGGCGGAGCACACGGAGCGGCTCAAGGCCAACGACAGCCTCAAGCTCTCCCAGGAGTATGAGTCCATCGACCCTGGACAGCAGTTCACATGGGAACATTCCAACCTGGAAGTGAACAAGCCGAAGAACCGCTATGCCAACGTCATCGCCTATGACCACTCCCGTGTCATCCTCCAGCCCATTGAAGGCATCATGGGCAGTGATTACATCAATGCCAACTACGTGGACGGCTACCGGCGTCAGAACGCGTACATTGCCACGCAGGGGCCGCTGCCTGAGACCTTTGGGGACTTCTGGCGTATGGTGTGGGAGCAGCGGTCGGCGACCATCGTCATGATGACGCGGCTGGAGGAGAAGTCACGGATCAAGTGTGATCAGTATTGGCCCAACAGAGGCACGGAGACCTACGGCTTCATCCAGGTCACGTTGCTAGATACCATCGAGCTGGCCACATTCTGCGTCAGGACATTCTCTCTGCACAAGAATGGCTCCAGTGAGAAACGCGAGGTCCGCCAGTTCCAGTTTACGGCGTGGCCGGACCATGGCGTGCCCGAATACCCAACGCCCTTCCTGGCTTTCCTGCGGAGAGTCAAGACCTGCAACCCGCCAGATGCCGGCCCCATCGTGGTTCACTGCAGTGCCGGTGTGGGCCGCACAGGCTGCTTTATCGTCATCGACGCCATGCTTGAGCGGATCAAGCCAGAGAAGACAGTCGATGTCTATGGCCACGTGACGCTCATGAGGTCCCAGCGCAACTACATGGTGCAGACGGAGGACCAGTACAGCTTCATCCACGAGGCCCTGCTGGAGGCCGTGGGCTGTGGCAACACAGAAGTGCCCGCACGCAGCCTCTATGCCTACATCCAGAAGCTGGCCCAGGTGGAGCCTGGCGAACACGTCACTGGCATGGAACTCGAGTTCAAGCGGCTGGCTAACTCCAAGGCCCACACGTCACGCTTCATCAGTGCCAATCTGCCTTGTAACAAGTTCAAGAACCGCCTGGTGAACATCATGCCCTATGAGAGCACACGGGTCTGTCTGCAACCCATCCGGGGTGTGGAGGGCTCTGACTACATCAACGCCAGCTTCATTGATGGCTACAGGCAGCAGAAGGCCTACATCGCGACACAGGGGCCGCTGGCGGAGACCACGGAAGACTTCTGGCGCATGCTGTGGGAGAACAATTCGACGATCGTGGTGATGCTGACCAAGCTGCGGGAGATGGGCCGGGAGAAGTGTCACCAGTACTGGCCGGCCGAGCGCTCTGCCCGCTACCAGTACTTTGTGGTAGATCCGATGGCAGAATACAACATGCCTCAGTATATCCTGCGAGAGTTCAAGGTCACAGATGCCCGGGATGGCCAGTCCCGGACTGTCCGGCAGTTCCAGTTCACAGACTGGCCGGAACAGGGTGTGCCAAAGTCGGGGGAGGGCTTCATCGACTTCATTGGCCAAGTGCATAAGACTAAGGAGCAGTTTGGCCAGGACGGCCCCATCTCTGTCCACTGCAGTGCCGGCGTGGGCAGGACGGGCGTCTTCATCACGCTTAGCATCGTGCTGGAGCGGATGCGGTATGAAGGCGTGGTGGACATCTTTCAGACGGTGAAGATGCTACGAACCCAGCGGCCGGCCATGGTGCAGACAGAGGATGAGTACCAGTTCTGTTACCAGGCGGCACTGGAGTACCTCGGAAGCTTTGACCACTATGCAACCTAAAGCCATGGTCCCCCCCAGGCCCGACACCACTGGCCCCGGATGCCTCTGCCCCTCCCGGGCGGACCTCCTGAGGCCTGGACCCCCAGTGGGCAGGGCAGGAGGTGGCAGCGGCAGCAGCTGTGTTTCTGCACCATTTCCGAGGACGACGCAGCCCCTCGAGCCCCCCCACCGGCCCCGGCCGCCCCAGCGACCTCCCTGGCACGGCCGCCGCCTTCAAATACTTGGCACATTCCTCCTTTCCTTCCAATTCCAAAACCAGATTCCGGGGTGGGGGGTGGGGGGATGGTGAGCAAATAGGAGTGCTCCCCAGAACCAGAGGAGGGTGGGGCACAGACCATAGACGGACCCCTCGTCCTCCCCCAGCGGTGGTAGGGGGACCCGGGGGGCTCCTCCCCGCTCTGCACC
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 75274, GC content failed 4722, low tm 13917, high tm 43873, high any compl 5, high end compl 24, high repeat similarity 30, long poly-x seq 219, ok 12484
PRIMER_RIGHT_EXPLAIN=considered 75198, GC content failed 4844, low tm 13849, high tm 43784, high any compl 4, high end compl 7, high repeat similarity 4, long poly-x seq 236, ok 12470
PRIMER_PAIR_EXPLAIN=considered 1800, unacceptable product size 1734, high end compl 11, ok 55
PRIMER_PAIR_PENALTY=0.0854
PRIMER_LEFT_PENALTY=0.052685
PRIMER_RIGHT_PENALTY=0.032744
PRIMER_LEFT_SEQUENCE=TCGAATATAAATCCAAGAGCCAA
PRIMER_RIGHT_SEQUENCE=GAAGAGGAGCTCGTACTTGATGA
PRIMER_LEFT=1341,23
PRIMER_RIGHT=1927,23
PRIMER_LEFT_TM=59.947
PRIMER_RIGHT_TM=60.033
PRIMER_LEFT_GC_PERCENT=34.783
PRIMER_RIGHT_GC_PERCENT=47.826
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_MISPRIMING_SCORE=11.00, MLT2D Interspersed repeat MLT2D - a consensus
PRIMER_RIGHT_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_END_STABILITY=10.0000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=587
PRIMER_PAIR_PENALTY_1=0.1393
PRIMER_LEFT_1_PENALTY=0.094334
PRIMER_RIGHT_1_PENALTY=0.044969
PRIMER_LEFT_1_SEQUENCE=TACAAGATCCAGTACAATGGGCT
PRIMER_RIGHT_1_SEQUENCE=GTTTACTGTCGGGTTTGTTCTTG
PRIMER_LEFT_1=3443,23
PRIMER_RIGHT_1=4187,23
PRIMER_LEFT_1_TM=59.906
PRIMER_RIGHT_1_TM=59.955
PRIMER_LEFT_1_GC_PERCENT=43.478
PRIMER_RIGHT_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_MISPRIMING_SCORE=11.00, reverse MER31 a consensus
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_1_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_1_END_STABILITY=10.9000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=745
PRIMER_PAIR_PENALTY_2=0.1393
PRIMER_LEFT_2_PENALTY=0.094334
PRIMER_RIGHT_2_PENALTY=0.044969
PRIMER_LEFT_2_SEQUENCE=CTACAAGATCCAGTACAATGGGC
PRIMER_RIGHT_2_SEQUENCE=GTTTACTGTCGGGTTTGTTCTTG
PRIMER_LEFT_2=3442,23
PRIMER_RIGHT_2=4187,23
PRIMER_LEFT_2_TM=59.906
PRIMER_RIGHT_2_TM=59.955
PRIMER_LEFT_2_GC_PERCENT=47.826
PRIMER_RIGHT_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_2_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_2_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_2_END_STABILITY=11.2000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=746
PRIMER_PAIR_PENALTY_3=0.1393
PRIMER_LEFT_3_PENALTY=0.094334
PRIMER_RIGHT_3_PENALTY=0.044969
PRIMER_LEFT_3_SEQUENCE=TACAAGATCCAGTACAATGGGCT
PRIMER_RIGHT_3_SEQUENCE=TTTACTGTCGGGTTTGTTCTTGT
PRIMER_LEFT_3=3443,23
PRIMER_RIGHT_3=4186,23
PRIMER_LEFT_3_TM=59.906
PRIMER_RIGHT_3_TM=59.955
PRIMER_LEFT_3_GC_PERCENT=43.478
PRIMER_RIGHT_3_GC_PERCENT=39.130
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_MISPRIMING_SCORE=11.00, reverse MER31 a consensus
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_3_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_3_END_STABILITY=10.9000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=744
PRIMER_PAIR_PENALTY_4=0.1393
PRIMER_LEFT_4_PENALTY=0.094334
PRIMER_RIGHT_4_PENALTY=0.044969
PRIMER_LEFT_4_SEQUENCE=CTACAAGATCCAGTACAATGGGC
PRIMER_RIGHT_4_SEQUENCE=TTTACTGTCGGGTTTGTTCTTGT
PRIMER_LEFT_4=3442,23
PRIMER_RIGHT_4=4186,23
PRIMER_LEFT_4_TM=59.906
PRIMER_RIGHT_4_TM=59.955
PRIMER_LEFT_4_GC_PERCENT=47.826
PRIMER_RIGHT_4_GC_PERCENT=39.130
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_4_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_4_END_STABILITY=11.2000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=745
PRIMER_PAIR_PENALTY_5=0.1758
PRIMER_LEFT_5_PENALTY=0.094334
PRIMER_RIGHT_5_PENALTY=0.081493
PRIMER_LEFT_5_SEQUENCE=CTACAAGATCCAGTACAATGGGC
PRIMER_RIGHT_5_SEQUENCE=TGTAGAGCAGGATAGCAATGACA
PRIMER_LEFT_5=3442,23
PRIMER_RIGHT_5=4166,23
PRIMER_LEFT_5_TM=59.906
PRIMER_RIGHT_5_TM=59.919
PRIMER_LEFT_5_GC_PERCENT=47.826
PRIMER_RIGHT_5_GC_PERCENT=43.478
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=2.00
PRIMER_RIGHT_5_SELF_END=3.00
PRIMER_LEFT_5_MISPRIMING_SCORE=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_5_MISPRIMING_SCORE=11.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_5_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_5_END_STABILITY=11.2000
PRIMER_RIGHT_5_END_STABILITY=6.7000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_5=725
PRIMER_PAIR_PENALTY_6=0.1758
PRIMER_LEFT_6_PENALTY=0.094334
PRIMER_RIGHT_6_PENALTY=0.081493
PRIMER_LEFT_6_SEQUENCE=TACAAGATCCAGTACAATGGGCT
PRIMER_RIGHT_6_SEQUENCE=TGTAGAGCAGGATAGCAATGACA
PRIMER_LEFT_6=3443,23
PRIMER_RIGHT_6=4166,23
PRIMER_LEFT_6_TM=59.906
PRIMER_RIGHT_6_TM=59.919
PRIMER_LEFT_6_GC_PERCENT=43.478
PRIMER_RIGHT_6_GC_PERCENT=43.478
PRIMER_LEFT_6_SELF_ANY=6.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=3.00
PRIMER_RIGHT_6_SELF_END=3.00
PRIMER_LEFT_6_MISPRIMING_SCORE=11.00, reverse MER31 a consensus
PRIMER_RIGHT_6_MISPRIMING_SCORE=11.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_6_MISPRIMING_SCORE=20.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_6_END_STABILITY=10.9000
PRIMER_RIGHT_6_END_STABILITY=6.7000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_6=724
PRIMER_PAIR_PENALTY_7=0.1761
PRIMER_LEFT_7_PENALTY=0.094334
PRIMER_RIGHT_7_PENALTY=0.081736
PRIMER_LEFT_7_SEQUENCE=TACAAGATCCAGTACAATGGGCT
PRIMER_RIGHT_7_SEQUENCE=ACTGTCGGGTTTGTTCTTGTAGA
PRIMER_LEFT_7=3443,23
PRIMER_RIGHT_7=4183,23
PRIMER_LEFT_7_TM=59.906
PRIMER_RIGHT_7_TM=60.082
PRIMER_LEFT_7_GC_PERCENT=43.478
PRIMER_RIGHT_7_GC_PERCENT=43.478
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=3.00
PRIMER_RIGHT_7_SELF_END=3.00
PRIMER_LEFT_7_MISPRIMING_SCORE=11.00, reverse MER31 a consensus
PRIMER_RIGHT_7_MISPRIMING_SCORE=12.00, MER34 a consensus
PRIMER_PAIR_7_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_7_END_STABILITY=10.9000
PRIMER_RIGHT_7_END_STABILITY=5.4000
PRIMER_PAIR_7_COMPL_ANY=7.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_7=741
PRIMER_PAIR_PENALTY_8=0.1761
PRIMER_LEFT_8_PENALTY=0.094334
PRIMER_RIGHT_8_PENALTY=0.081736
PRIMER_LEFT_8_SEQUENCE=CTACAAGATCCAGTACAATGGGC
PRIMER_RIGHT_8_SEQUENCE=ACTGTCGGGTTTGTTCTTGTAGA
PRIMER_LEFT_8=3442,23
PRIMER_RIGHT_8=4183,23
PRIMER_LEFT_8_TM=59.906
PRIMER_RIGHT_8_TM=60.082
PRIMER_LEFT_8_GC_PERCENT=47.826
PRIMER_RIGHT_8_GC_PERCENT=43.478
PRIMER_LEFT_8_SELF_ANY=6.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=2.00
PRIMER_RIGHT_8_SELF_END=3.00
PRIMER_LEFT_8_MISPRIMING_SCORE=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, MER34 a consensus
PRIMER_PAIR_8_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_8_END_STABILITY=11.2000
PRIMER_RIGHT_8_END_STABILITY=5.4000
PRIMER_PAIR_8_COMPL_ANY=8.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_8=742
PRIMER_PAIR_PENALTY_9=0.2345
PRIMER_LEFT_9_PENALTY=0.101745
PRIMER_RIGHT_9_PENALTY=0.132718
PRIMER_LEFT_9_SEQUENCE=GGTTTATCAAAGAACCCAAGGAC
PRIMER_RIGHT_9_SEQUENCE=TCTCATCAAACTCAATCGTCTCA
PRIMER_LEFT_9=348,23
PRIMER_RIGHT_9=503,23
PRIMER_LEFT_9_TM=60.102
PRIMER_RIGHT_9_TM=59.867
PRIMER_LEFT_9_GC_PERCENT=43.478
PRIMER_RIGHT_9_GC_PERCENT=39.130
PRIMER_LEFT_9_SELF_ANY=5.00
PRIMER_RIGHT_9_SELF_ANY=2.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_MISPRIMING_SCORE=12.00, MLT1c (MLT1c subfamily) - consensus sequence
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_9_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_9_END_STABILITY=7.6000
PRIMER_RIGHT_9_END_STABILITY=6.7000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_9=156
PRIMER_PAIR_PENALTY_10=0.2345
PRIMER_LEFT_10_PENALTY=0.101745
PRIMER_RIGHT_10_PENALTY=0.132718
PRIMER_LEFT_10_SEQUENCE=GTTTATCAAAGAACCCAAGGACC
PRIMER_RIGHT_10_SEQUENCE=TCTCATCAAACTCAATCGTCTCA
PRIMER_LEFT_10=349,23
PRIMER_RIGHT_10=503,23
PRIMER_LEFT_10_TM=60.102
PRIMER_RIGHT_10_TM=59.867
PRIMER_LEFT_10_GC_PERCENT=43.478
PRIMER_RIGHT_10_GC_PERCENT=39.130
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=2.00
PRIMER_LEFT_10_SELF_END=3.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_MISPRIMING_SCORE=11.00, MSTc consensus sequence
PRIMER_RIGHT_10_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_10_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_10_END_STABILITY=9.1000
PRIMER_RIGHT_10_END_STABILITY=6.7000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_10=155
PRIMER_PAIR_PENALTY_11=0.2419
PRIMER_LEFT_11_PENALTY=0.160360
PRIMER_RIGHT_11_PENALTY=0.081493
PRIMER_LEFT_11_SEQUENCE=AACTACAACTCACCCACACCCTA
PRIMER_RIGHT_11_SEQUENCE=TGTAGAGCAGGATAGCAATGACA
PRIMER_LEFT_11=3422,23
PRIMER_RIGHT_11=4166,23
PRIMER_LEFT_11_TM=59.840
PRIMER_RIGHT_11_TM=59.919
PRIMER_LEFT_11_GC_PERCENT=47.826
PRIMER_RIGHT_11_GC_PERCENT=43.478
PRIMER_LEFT_11_SELF_ANY=2.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=2.00
PRIMER_RIGHT_11_SELF_END=3.00
PRIMER_LEFT_11_MISPRIMING_SCORE=12.00, reverse MER4C a consensus
PRIMER_RIGHT_11_MISPRIMING_SCORE=11.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_11_MISPRIMING_SCORE=23.00, reverse MER4C a consensus
PRIMER_LEFT_11_END_STABILITY=8.7000
PRIMER_RIGHT_11_END_STABILITY=6.7000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_11=745
PRIMER_PAIR_PENALTY_12=0.2421
PRIMER_LEFT_12_PENALTY=0.160360
PRIMER_RIGHT_12_PENALTY=0.081736
PRIMER_LEFT_12_SEQUENCE=AACTACAACTCACCCACACCCTA
PRIMER_RIGHT_12_SEQUENCE=ACTGTCGGGTTTGTTCTTGTAGA
PRIMER_LEFT_12=3422,23
PRIMER_RIGHT_12=4183,23
PRIMER_LEFT_12_TM=59.840
PRIMER_RIGHT_12_TM=60.082
PRIMER_LEFT_12_GC_PERCENT=47.826
PRIMER_RIGHT_12_GC_PERCENT=43.478
PRIMER_LEFT_12_SELF_ANY=2.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=2.00
PRIMER_RIGHT_12_SELF_END=3.00
PRIMER_LEFT_12_MISPRIMING_SCORE=12.00, reverse MER4C a consensus
PRIMER_RIGHT_12_MISPRIMING_SCORE=12.00, MER34 a consensus
PRIMER_PAIR_12_MISPRIMING_SCORE=22.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_12_END_STABILITY=8.7000
PRIMER_RIGHT_12_END_STABILITY=5.4000
PRIMER_PAIR_12_COMPL_ANY=7.00
PRIMER_PAIR_12_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_12=762
PRIMER_PAIR_PENALTY_13=0.2569
PRIMER_LEFT_13_PENALTY=0.211969
PRIMER_RIGHT_13_PENALTY=0.044969
PRIMER_LEFT_13_SEQUENCE=CCTTCTACAACTTTGTGCTGACC
PRIMER_RIGHT_13_SEQUENCE=TTTACTGTCGGGTTTGTTCTTGT
PRIMER_LEFT_13=3525,23
PRIMER_RIGHT_13=4186,23
PRIMER_LEFT_13_TM=60.212
PRIMER_RIGHT_13_TM=59.955
PRIMER_LEFT_13_GC_PERCENT=47.826
PRIMER_RIGHT_13_GC_PERCENT=39.130
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_MISPRIMING_SCORE=12.00, Tigger2 Autonomous DNA transposon
PRIMER_RIGHT_13_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_13_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_13_END_STABILITY=7.9000
PRIMER_RIGHT_13_END_STABILITY=6.7000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_13=662
PRIMER_PAIR_PENALTY_14=0.2569
PRIMER_LEFT_14_PENALTY=0.211969
PRIMER_RIGHT_14_PENALTY=0.044969
PRIMER_LEFT_14_SEQUENCE=CCTTCTACAACTTTGTGCTGACC
PRIMER_RIGHT_14_SEQUENCE=GTTTACTGTCGGGTTTGTTCTTG
PRIMER_LEFT_14=3525,23
PRIMER_RIGHT_14=4187,23
PRIMER_LEFT_14_TM=60.212
PRIMER_RIGHT_14_TM=59.955
PRIMER_LEFT_14_GC_PERCENT=47.826
PRIMER_RIGHT_14_GC_PERCENT=43.478
PRIMER_LEFT_14_SELF_ANY=6.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_MISPRIMING_SCORE=12.00, Tigger2 Autonomous DNA transposon
PRIMER_RIGHT_14_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_14_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_14_END_STABILITY=7.9000
PRIMER_RIGHT_14_END_STABILITY=7.0000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_14=663
PRIMER_PAIR_PENALTY_15=0.2749
PRIMER_LEFT_15_PENALTY=0.229951
PRIMER_RIGHT_15_PENALTY=0.044969
PRIMER_LEFT_15_SEQUENCE=CCTACAAGATCCAGTACAATGGG
PRIMER_RIGHT_15_SEQUENCE=GTTTACTGTCGGGTTTGTTCTTG
PRIMER_LEFT_15=3441,23
PRIMER_RIGHT_15=4187,23
PRIMER_LEFT_15_TM=59.770
PRIMER_RIGHT_15_TM=59.955
PRIMER_LEFT_15_GC_PERCENT=47.826
PRIMER_RIGHT_15_GC_PERCENT=43.478
PRIMER_LEFT_15_SELF_ANY=6.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=2.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_MISPRIMING_SCORE=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_15_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_15_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_15_END_STABILITY=9.6000
PRIMER_RIGHT_15_END_STABILITY=7.0000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_15=747
PRIMER_PAIR_PENALTY_16=0.2749
PRIMER_LEFT_16_PENALTY=0.229951
PRIMER_RIGHT_16_PENALTY=0.044969
PRIMER_LEFT_16_SEQUENCE=CCTACAAGATCCAGTACAATGGG
PRIMER_RIGHT_16_SEQUENCE=TTTACTGTCGGGTTTGTTCTTGT
PRIMER_LEFT_16=3441,23
PRIMER_RIGHT_16=4186,23
PRIMER_LEFT_16_TM=59.770
PRIMER_RIGHT_16_TM=59.955
PRIMER_LEFT_16_GC_PERCENT=47.826
PRIMER_RIGHT_16_GC_PERCENT=39.130
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=3.00
PRIMER_LEFT_16_SELF_END=2.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_MISPRIMING_SCORE=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_16_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_16_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_16_END_STABILITY=9.6000
PRIMER_RIGHT_16_END_STABILITY=6.7000
PRIMER_PAIR_16_COMPL_ANY=6.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_16=746
PRIMER_PAIR_PENALTY_17=0.2935
PRIMER_LEFT_17_PENALTY=0.211969
PRIMER_RIGHT_17_PENALTY=0.081493
PRIMER_LEFT_17_SEQUENCE=CCTTCTACAACTTTGTGCTGACC
PRIMER_RIGHT_17_SEQUENCE=TGTAGAGCAGGATAGCAATGACA
PRIMER_LEFT_17=3525,23
PRIMER_RIGHT_17=4166,23
PRIMER_LEFT_17_TM=60.212
PRIMER_RIGHT_17_TM=59.919
PRIMER_LEFT_17_GC_PERCENT=47.826
PRIMER_RIGHT_17_GC_PERCENT=43.478
PRIMER_LEFT_17_SELF_ANY=6.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=3.00
PRIMER_LEFT_17_MISPRIMING_SCORE=12.00, Tigger2 Autonomous DNA transposon
PRIMER_RIGHT_17_MISPRIMING_SCORE=11.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_17_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_17_END_STABILITY=7.9000
PRIMER_RIGHT_17_END_STABILITY=6.7000
PRIMER_PAIR_17_COMPL_ANY=6.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_17=642
PRIMER_PAIR_PENALTY_18=0.2937
PRIMER_LEFT_18_PENALTY=0.211969
PRIMER_RIGHT_18_PENALTY=0.081736
PRIMER_LEFT_18_SEQUENCE=CCTTCTACAACTTTGTGCTGACC
PRIMER_RIGHT_18_SEQUENCE=ACTGTCGGGTTTGTTCTTGTAGA
PRIMER_LEFT_18=3525,23
PRIMER_RIGHT_18=4183,23
PRIMER_LEFT_18_TM=60.212
PRIMER_RIGHT_18_TM=60.082
PRIMER_LEFT_18_GC_PERCENT=47.826
PRIMER_RIGHT_18_GC_PERCENT=43.478
PRIMER_LEFT_18_SELF_ANY=6.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=3.00
PRIMER_LEFT_18_MISPRIMING_SCORE=12.00, Tigger2 Autonomous DNA transposon
PRIMER_RIGHT_18_MISPRIMING_SCORE=12.00, MER34 a consensus
PRIMER_PAIR_18_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_18_END_STABILITY=7.9000
PRIMER_RIGHT_18_END_STABILITY=5.4000
PRIMER_PAIR_18_COMPL_ANY=7.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_18=659
PRIMER_PAIR_PENALTY_19=0.2990
PRIMER_LEFT_19_PENALTY=0.094334
PRIMER_RIGHT_19_PENALTY=0.204708
PRIMER_LEFT_19_SEQUENCE=CTACAAGATCCAGTACAATGGGC
PRIMER_RIGHT_19_SEQUENCE=CTGTCGGGTTTGTTCTTGTAGAG
PRIMER_LEFT_19=3442,23
PRIMER_RIGHT_19=4182,23
PRIMER_LEFT_19_TM=59.906
PRIMER_RIGHT_19_TM=60.205
PRIMER_LEFT_19_GC_PERCENT=47.826
PRIMER_RIGHT_19_GC_PERCENT=47.826
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=2.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_MISPRIMING_SCORE=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_19_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_19_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_19_END_STABILITY=11.2000
PRIMER_RIGHT_19_END_STABILITY=5.7000
PRIMER_PAIR_19_COMPL_ANY=8.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_19=741
=