PRIMER_SEQUENCE_ID=NM_003463
SEQUENCE=CCGGCTCGGTACGCGCTCTGCTCCGAGCCGCTCACTGCGTGGTAGAGTCTGGTGCCCCCGCCGTCGCCTGCATCGCCGCCACCGCCGCTCCGCCACGACCACCGCCGCCTCCTGCCCTGCAGCCACCGCCACCGCCTGTGTCGCCGCCGCCTCGGGACCGGCTGTATGATTAGGCCACAATCTTCAATGAGTAAACATATTCCTCAATTCTGTGGTGTTCTTGGTCACACATTTATGGAGTTTCTGAAGGGCAGTGGAGATTACTGCCAGGCACAGCACGACCTCTATGCAGACAAGTGAACTGTAGAAACTGATTACTGCTCCACCAAGAAGCCCCCATAAGAGTGGTTATCCTGGACACAGAAGTGTTGAATTGAAATCCACAGAGCATTTTACAAGAGTTCTGACCTGGATGGGGTAAACCTCAGTGCACTTCTTTTCTGTTGGCCTCAGTATTACTGGATTGAAGAATTGCTGCTTCTTGTTAGGAGGTTCATTTCACTTATCATTACTTACAACTTCATACTCAAAGCACTGAGAATTTCAAGTGGAGTATATTGAAGTAGACTTCAGTTTCTTTGCATCATTTCTGTATTCAATTTTTTTAATTATTTCATAACCCTATTGAGTGTTTTTTAACTAAATTAACATGGCTCGAATGAACCGCCCAGCTCCTGTGGAAGTCACATACAAGAACATGAGATTTCTTATTACACACAATCCAACCAATGCGACCTTAAACAAATTTATAGAGGAACTTAAGAAGTATGGAGTTACCACAATAGTAAGAGTATGTGAAGCAACTTATGACACTACTCTTGTGGAGAAAGAAGGTATCCATGTTCTTGATTGGCCTTTTGATGATGGTGCACCACCATCCAACCAGATTGTTGATGACTGGTTAAGTCTTGTGAAAATTAAGTTTCGTGAAGAACCTGGTTGTTGTATTGCTGTTCATTGCGTTGCAGGCCTTGGGAGAGCTCCAGTACTTGTTGCCCTAGCATTAATTGAAGGTGGAATGAAATACGAAGATGCAGTACAATTCATAAGACAAAAGCGGCGTGGAGCTTTTAACAGCAAGCAACTTCTGTATTTGGAGAAGTATCGTCCTAAAATGCGGCTGCGTTTCAAAGATTCCAACGGTCATAGAAACAACTGTTGCATTCAATAAAATTGGGGTGCCTAATGCTACTGGAAGTGGAACTTGAGATAGGGCCTAATTTGTTATACATATTAGCCAACATGTTGGCTTAGTAAGTCTAATGAAGCTTCCATAGGAGTATTGAAAGGCAGTTTTACCAGGCCTCAAGCTAGACAGATTTGGCAACCTCTGTATTTGGGTTACAGTCAACCTATTTGGATACTTGGCAAAAGATTCTTGCTGTCAGCATATAAAATGTGCTTGTCATTTGTATCAATTGACCTTTCCCCAAATCATGCAGTATTGAGTTATGACTTGTTAAATCTATTCCCATGCCAGAATCTTATCAATACATAAGAAATTTAGGAAGATTAGGTGCCAAAATACCCAGCACAATACTTGTATATTTTTAGTACCATACAGAAGTAAAATCCCAGGAACTATGAACACTAGACCTTATGTGGTTTATTCCTTCAATCATTTCAAACATTGAAAGTAGGGCCTACATGGTTATTTGCCTGCTCACTTTATGTTTACATCTCCCACATTCATACCAATATACGTCAGGTTTGCTTAACCATTGATTTTTTTTTTTTTTTACCAAGTCTTACAGTGATTATTTTACGTGTTTCCATGTATCTCACTTTGTGCTGTATTAAAAAAACCTCCATTTTGAAAATCTACGTTGTACAGAAGCACATGTCTTTAATGTCTTCAGACAAAAAAGCCTTACATTAATTTAATGTTTGCACTCTGAGGTGCAACTTAACAGGGAGGGCCTGAGAAAAGAATGGGAGGGGGCTATTAATTATTTTTTAGCAAAATGTTGCCTTTGTCTTGTGCAAACATGTAGAATATGCTCTTTAATTTAGTAAAATATTTTTTTAAAAGGTAGAGATGCTTTGTTATTGTAATCATAAACTTCCTGAAATTCTTGTAATTTTTTTCCCATACTTATCAGAAGTGTGTTTACCAACTTATTTTTGTTTGAAAGTGTGATTTTTTTTTTCCTTCCCAACCTCTCTTGCAAAAAAAGAAATGGGTTTCTGCTAATGAATTGAGCACATCTAATATTTTATATGCCTTTTGGAGCTGTGTAAGTTAATATTTGATACTTGACAATTTGTTTTATTATGTAATTGATAAAATGGTGATGTGTATTAATGTTAGTTCAACCATATATTTATACTGTCTGGGGATGTGTGGTTATAGTTCTGTGGGAGAAATAATTTTGTCAGTGTTCACCAGCTTGTAAAAACTTAGTGCGAGAGCTGAAACATCTAAATAAATAATGACATGCATTTATCATCATTGAGATTGGTTTGCTTTAAATTAACTTATTTTGTAGAAGACAAAATGAATTGCACTTCACTTAATGTGTGTCCTCATCTTTTTACAAATAAATGAAGGATTATAAATGATGTCAGCATTTTAGTAAACTTTTAGACAAAATTTGTTAGGGTCATTCATGAAAACTTTAATACTAAAAGCACTTTCCATTATATACTTTTTAAAGGTCTAGATAATTTTGAACCAATTTATTATTGTGTACTGAGGAGAAATAATGTATAGTAGAGGACAGCCTTGGTTTGTAAAGCTCAGTTCCACTAGTTCATGGTTTTGTGCAACTTCTGAGCCTCAGTTTTCTCCTTTGCAAATTAATAATTACATACCTTTATAGATTTTGAAATTAATTTAAATATTAGTATTTGGTACATGAAGGCTTAATGTTAAGTTTCCTTTAATGATCCACAATAATCCCTTTGATCACGTTAATCTAAATCTAGATGTCTTTGTCTAATTTTTTTTGAATAGCAGTTATAAATGTAAAGGACTCAAAGTTTAAGTAAAAAGTGATACTCCACCTTGTGTTTCAAAGAATTTAGTTCCACCTCTTCATACCAGTTTAACACTTAATATATTTCATTGGATTTTAGACAGGGCAAAAGGAAGAACAGGGGCCTCTGGAGGCCCTTGGTTATTTAAATCTTGGATTATTTGTGATAGTAATCACAAATTTTTGGCTAATTTTTAACCTGAGGTTTTGTTTTTTTTTTAAAGGAAATGCAGCCTAGTCTTGAGAACATAATTTTATATAATCAATTACTAAATGTTAAACTATTACCACACAGCCCATAAAACAGCATTTGCGTTTATTGAGAGAGAGGATGTGCCATCATGATTAATGAAAACTATCTTTTGAGTTTGAAAAGAAATTAATTTGCAGTGTTTGGATTGTATATATGGTGCTAAAAATAAATTAATTTACTTTATAAACCTTATCTGTACATTATACGATGTGATGAAATTTGCTTTTTATCCAAATATTTTGTATCTTGTAAATATGGCTAATTATAGGAATGCCTATAATACATCTTAGATTCCTTATATCTAATAAGAGTTCAAAGAGTTATGAGTTGAAGTCTTGAATGCAGGAAACTATCTGATAGTGTTCTAAAATTTGGTTACTTGGGTTTGGATACCCTTAGTGGGATGATGTAAATAGAGGCTAGCTACCTAGGCTTGTCTATAGCAACCATAATGTTGATGTAAGTAATGCGGTTACTGAATCATAAGAAAATGCCATCTCTTTTTAGTTGAAGGAAAACTCTGGAAGTAGGTGCCATTGGTCATTCTGCAGTGCACTGCAACCATTGTTTCCCCTAGTGCCCTCTTTTCCCTAGGGCATTGCTCTCCTATTCCCACGCCTTAACACAGCTCTATACCTAGAAGCAGCCAGCCCAGGCATGCAGTCACATTTAATCACATCCCCCTTCTAGAGTGCTTCAAAATGATGTAGTCCCTCAACTTGGCTAAAGAATCTCAATCTCTTGAAATTTATTTTTTTAATGTCATATTCATCTGGTAAATATCTACTGTTTGCCAGGCATTTAAGAATATGGCAAAGAACATAAAAGATGGTGTCACCAGATTTTGGTCACCAATGAGTACCCGACCCGTTGCCATGATTAAGAGAGAATGCTTTCTATTGGAGTTTCAGGAAATATAATTTGAGAATACTTTAAAGGGAAGTGGAAGTATAAGTGAATGATATTTTTCTTTTACATGTAAACAATGAAGTTATTTCAAAGTTAAGTTTTAAACAAAATACATGAAGTAGTGTCTGCCATACATGTTAATATTCTACATTCTTGCTTCCTTAAATTAATATGTTTGTGTGTATATATGTGCCTCACACCTGAATTGAAAATTAAAGACTGGTTTAAAAGTGGTTAAAAAAAAAAAAAAAAAA
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 50016, GC content failed 5225, low tm 30357, high tm 5602, high any compl 5, high end compl 35, high repeat similarity 51, long poly-x seq 231, ok 8510
PRIMER_RIGHT_EXPLAIN=considered 49941, GC content failed 5190, low tm 30845, high tm 5048, high any compl 3, high end compl 12, high repeat similarity 11, long poly-x seq 240, ok 8592
PRIMER_PAIR_EXPLAIN=considered 2453, unacceptable product size 2381, high end compl 10, ok 62
PRIMER_PAIR_PENALTY=0.0427
PRIMER_LEFT_PENALTY=0.014220
PRIMER_RIGHT_PENALTY=0.028502
PRIMER_LEFT_SEQUENCE=TTAGGCCACAATCTTCAATGAGT
PRIMER_RIGHT_SEQUENCE=AACAGAAAAGAAGTGCACTGAGG
PRIMER_LEFT=169,23
PRIMER_RIGHT=444,23
PRIMER_LEFT_TM=60.014
PRIMER_RIGHT_TM=59.971
PRIMER_LEFT_GC_PERCENT=39.130
PRIMER_RIGHT_GC_PERCENT=43.478
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=276
PRIMER_PAIR_PENALTY_1=0.0507
PRIMER_LEFT_1_PENALTY=0.014220
PRIMER_RIGHT_1_PENALTY=0.036444
PRIMER_LEFT_1_SEQUENCE=TGCTTCAAAATGATGTAGTCCCT
PRIMER_RIGHT_1_SEQUENCE=AAATGCCTGGCAAACAGTAGATA
PRIMER_LEFT_1=3923,23
PRIMER_RIGHT_1=4033,23
PRIMER_LEFT_1_TM=60.014
PRIMER_RIGHT_1_TM=60.036
PRIMER_LEFT_1_GC_PERCENT=39.130
PRIMER_RIGHT_1_GC_PERCENT=39.130
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_MISPRIMING_SCORE=11.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_1_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_1_MISPRIMING_SCORE=21.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=5.6000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=111
PRIMER_PAIR_PENALTY_2=0.0507
PRIMER_LEFT_2_PENALTY=0.014220
PRIMER_RIGHT_2_PENALTY=0.036444
PRIMER_LEFT_2_SEQUENCE=TGCTTCAAAATGATGTAGTCCCT
PRIMER_RIGHT_2_SEQUENCE=TAAATGCCTGGCAAACAGTAGAT
PRIMER_LEFT_2=3923,23
PRIMER_RIGHT_2=4034,23
PRIMER_LEFT_2_TM=60.014
PRIMER_RIGHT_2_TM=60.036
PRIMER_LEFT_2_GC_PERCENT=39.130
PRIMER_RIGHT_2_GC_PERCENT=39.130
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_MISPRIMING_SCORE=11.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_2_MISPRIMING_SCORE=11.00, reverse MIR2 Repetitive element - a consensus
PRIMER_PAIR_2_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=5.6000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=112
PRIMER_PAIR_PENALTY_3=0.0909
PRIMER_LEFT_3_PENALTY=0.054491
PRIMER_RIGHT_3_PENALTY=0.036444
PRIMER_LEFT_3_SEQUENCE=TACGTCAGGTTTGCTTAACCATT
PRIMER_RIGHT_3_SEQUENCE=ACACAGCTCCAAAAGGCATATAA
PRIMER_LEFT_3=1700,23
PRIMER_RIGHT_3=2238,23
PRIMER_LEFT_3_TM=59.946
PRIMER_RIGHT_3_TM=60.036
PRIMER_LEFT_3_GC_PERCENT=39.130
PRIMER_RIGHT_3_GC_PERCENT=39.130
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_3_MISPRIMING_SCORE=11.00, MSTa consensus sequence
PRIMER_PAIR_3_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=5.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=539
PRIMER_PAIR_PENALTY_4=0.0927
PRIMER_LEFT_4_PENALTY=0.028502
PRIMER_RIGHT_4_PENALTY=0.064245
PRIMER_LEFT_4_SEQUENCE=AACCTCAGTGCACTTCTTTTCTG
PRIMER_RIGHT_4_SEQUENCE=TCTCAAGTTCCACTTCCAGTAGC
PRIMER_LEFT_4=420,23
PRIMER_RIGHT_4=1209,23
PRIMER_LEFT_4_TM=59.971
PRIMER_RIGHT_4_TM=59.936
PRIMER_LEFT_4_GC_PERCENT=43.478
PRIMER_RIGHT_4_GC_PERCENT=47.826
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, SVA Composite retroposon
PRIMER_RIGHT_4_MISPRIMING_SCORE=11.00, L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence
PRIMER_PAIR_4_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=790
PRIMER_PAIR_PENALTY_5=0.0940
PRIMER_LEFT_5_PENALTY=0.014220
PRIMER_RIGHT_5_PENALTY=0.079770
PRIMER_LEFT_5_SEQUENCE=TTAGGCCACAATCTTCAATGAGT
PRIMER_RIGHT_5_SEQUENCE=AGTTGTTTCTATGACCGTTGGAA
PRIMER_LEFT_5=169,23
PRIMER_RIGHT_5=1156,23
PRIMER_LEFT_5_TM=60.014
PRIMER_RIGHT_5_TM=59.920
PRIMER_LEFT_5_GC_PERCENT=39.130
PRIMER_RIGHT_5_GC_PERCENT=39.130
PRIMER_LEFT_5_SELF_ANY=5.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=3.00
PRIMER_RIGHT_5_SELF_END=2.00
PRIMER_LEFT_5_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_5_MISPRIMING_SCORE=11.00, reverse MER21B a consensus
PRIMER_PAIR_5_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_5_END_STABILITY=6.4000
PRIMER_RIGHT_5_END_STABILITY=8.5000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_5=988
PRIMER_PAIR_PENALTY_6=0.0957
PRIMER_LEFT_6_PENALTY=0.061028
PRIMER_RIGHT_6_PENALTY=0.034654
PRIMER_LEFT_6_SEQUENCE=ACTCCACCTTGTGTTTCAAAGAA
PRIMER_RIGHT_6_SEQUENCE=TTCTCAAGACTAGGCTGCATTTC
PRIMER_LEFT_6=2999,23
PRIMER_RIGHT_6=3224,23
PRIMER_LEFT_6_TM=60.061
PRIMER_RIGHT_6_TM=60.035
PRIMER_LEFT_6_GC_PERCENT=39.130
PRIMER_RIGHT_6_GC_PERCENT=43.478
PRIMER_LEFT_6_SELF_ANY=5.00
PRIMER_RIGHT_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=3.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_MISPRIMING_SCORE=12.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_6_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_6_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_6_END_STABILITY=7.0000
PRIMER_RIGHT_6_END_STABILITY=6.9000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_6=226
PRIMER_PAIR_PENALTY_7=0.0971
PRIMER_LEFT_7_PENALTY=0.082838
PRIMER_RIGHT_7_PENALTY=0.014220
PRIMER_LEFT_7_SEQUENCE=GCCCTTGGTTATTTAAATCTTGG
PRIMER_RIGHT_7_SEQUENCE=AGGGACTACATCATTTTGAAGCA
PRIMER_LEFT_7=3111,23
PRIMER_RIGHT_7=3945,23
PRIMER_LEFT_7_TM=60.083
PRIMER_RIGHT_7_TM=60.014
PRIMER_LEFT_7_GC_PERCENT=39.130
PRIMER_RIGHT_7_GC_PERCENT=39.130
PRIMER_LEFT_7_SELF_ANY=8.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=3.00
PRIMER_LEFT_7_MISPRIMING_SCORE=12.00, reverse L1MA2 3'-end of L1 repeat (subfamily L1MA2) - a consensus sequence
PRIMER_RIGHT_7_MISPRIMING_SCORE=11.00, L1MC2 3'-end of L1 repeat (subfamily L1MC2) - a consensus sequence
PRIMER_PAIR_7_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_7_END_STABILITY=8.5000
PRIMER_RIGHT_7_END_STABILITY=8.5000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_7=835
PRIMER_PAIR_PENALTY_8=0.0975
PRIMER_LEFT_8_PENALTY=0.036444
PRIMER_RIGHT_8_PENALTY=0.061028
PRIMER_LEFT_8_SEQUENCE=TTATATGCCTTTTGGAGCTGTGT
PRIMER_RIGHT_8_SEQUENCE=TTCTTTGAAACACAAGGTGGAGT
PRIMER_LEFT_8=2216,23
PRIMER_RIGHT_8=3021,23
PRIMER_LEFT_8_TM=60.036
PRIMER_RIGHT_8_TM=60.061
PRIMER_LEFT_8_GC_PERCENT=39.130
PRIMER_RIGHT_8_GC_PERCENT=39.130
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=5.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=2.00
PRIMER_LEFT_8_MISPRIMING_SCORE=12.00, THE1BR THE-1B Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_8_MISPRIMING_SCORE=22.00, MSTAR MSTa-Mammalian LTR internal retrotransposon sequence - a consensus
PRIMER_LEFT_8_END_STABILITY=6.4000
PRIMER_RIGHT_8_END_STABILITY=7.6000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_8=806
PRIMER_PAIR_PENALTY_9=0.0975
PRIMER_LEFT_9_PENALTY=0.036444
PRIMER_RIGHT_9_PENALTY=0.061028
PRIMER_LEFT_9_SEQUENCE=ATATGCCTTTTGGAGCTGTGTAA
PRIMER_RIGHT_9_SEQUENCE=TTCTTTGAAACACAAGGTGGAGT
PRIMER_LEFT_9=2218,23
PRIMER_RIGHT_9=3021,23
PRIMER_LEFT_9_TM=60.036
PRIMER_RIGHT_9_TM=60.061
PRIMER_LEFT_9_GC_PERCENT=39.130
PRIMER_RIGHT_9_GC_PERCENT=39.130
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=5.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=2.00
PRIMER_LEFT_9_MISPRIMING_SCORE=12.00, THE1BR THE-1B Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_9_MISPRIMING_SCORE=22.00, MSTAR MSTa-Mammalian LTR internal retrotransposon sequence - a consensus
PRIMER_LEFT_9_END_STABILITY=6.0000
PRIMER_RIGHT_9_END_STABILITY=7.6000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_9=804
PRIMER_PAIR_PENALTY_10=0.1058
PRIMER_LEFT_10_PENALTY=0.026007
PRIMER_RIGHT_10_PENALTY=0.079770
PRIMER_LEFT_10_SEQUENCE=TTCCTCAATTCTGTGGTGTTCTT
PRIMER_RIGHT_10_SEQUENCE=AGTTGTTTCTATGACCGTTGGAA
PRIMER_LEFT_10=199,23
PRIMER_RIGHT_10=1156,23
PRIMER_LEFT_10_TM=60.026
PRIMER_RIGHT_10_TM=59.920
PRIMER_LEFT_10_GC_PERCENT=39.130
PRIMER_RIGHT_10_GC_PERCENT=39.130
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=2.00
PRIMER_LEFT_10_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_10_MISPRIMING_SCORE=11.00, reverse MER21B a consensus
PRIMER_PAIR_10_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_10_END_STABILITY=7.0000
PRIMER_RIGHT_10_END_STABILITY=8.5000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_10=958
PRIMER_PAIR_PENALTY_11=0.1083
PRIMER_LEFT_11_PENALTY=0.028502
PRIMER_RIGHT_11_PENALTY=0.079770
PRIMER_LEFT_11_SEQUENCE=AACCTCAGTGCACTTCTTTTCTG
PRIMER_RIGHT_11_SEQUENCE=AGTTGTTTCTATGACCGTTGGAA
PRIMER_LEFT_11=420,23
PRIMER_RIGHT_11=1156,23
PRIMER_LEFT_11_TM=59.971
PRIMER_RIGHT_11_TM=59.920
PRIMER_LEFT_11_GC_PERCENT=43.478
PRIMER_RIGHT_11_GC_PERCENT=39.130
PRIMER_LEFT_11_SELF_ANY=8.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=2.00
PRIMER_LEFT_11_MISPRIMING_SCORE=12.00, SVA Composite retroposon
PRIMER_RIGHT_11_MISPRIMING_SCORE=11.00, reverse MER21B a consensus
PRIMER_PAIR_11_MISPRIMING_SCORE=21.00, reverse MER21B a consensus
PRIMER_LEFT_11_END_STABILITY=7.0000
PRIMER_RIGHT_11_END_STABILITY=8.5000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_11=737
PRIMER_PAIR_PENALTY_12=0.1169
PRIMER_LEFT_12_PENALTY=0.102704
PRIMER_RIGHT_12_PENALTY=0.014220
PRIMER_LEFT_12_SEQUENCE=ACGATGTGATGAAATTTGCTTTT
PRIMER_RIGHT_12_SEQUENCE=AGGGACTACATCATTTTGAAGCA
PRIMER_LEFT_12=3435,23
PRIMER_RIGHT_12=3945,23
PRIMER_LEFT_12_TM=59.897
PRIMER_RIGHT_12_TM=60.014
PRIMER_LEFT_12_GC_PERCENT=30.435
PRIMER_RIGHT_12_GC_PERCENT=39.130
PRIMER_LEFT_12_SELF_ANY=6.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=3.00
PRIMER_LEFT_12_MISPRIMING_SCORE=12.00, MER7B Nonautonomous DNA transposon
PRIMER_RIGHT_12_MISPRIMING_SCORE=11.00, L1MC2 3'-end of L1 repeat (subfamily L1MC2) - a consensus sequence
PRIMER_PAIR_12_MISPRIMING_SCORE=20.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_12_END_STABILITY=7.3000
PRIMER_RIGHT_12_END_STABILITY=8.5000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_12=511
PRIMER_PAIR_PENALTY_13=0.1175
PRIMER_LEFT_13_PENALTY=0.082838
PRIMER_RIGHT_13_PENALTY=0.034654
PRIMER_LEFT_13_SEQUENCE=GCCCTTGGTTATTTAAATCTTGG
PRIMER_RIGHT_13_SEQUENCE=TTCTCAAGACTAGGCTGCATTTC
PRIMER_LEFT_13=3111,23
PRIMER_RIGHT_13=3224,23
PRIMER_LEFT_13_TM=60.083
PRIMER_RIGHT_13_TM=60.035
PRIMER_LEFT_13_GC_PERCENT=39.130
PRIMER_RIGHT_13_GC_PERCENT=43.478
PRIMER_LEFT_13_SELF_ANY=8.00
PRIMER_RIGHT_13_SELF_ANY=6.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_MISPRIMING_SCORE=12.00, reverse L1MA2 3'-end of L1 repeat (subfamily L1MA2) - a consensus sequence
PRIMER_RIGHT_13_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_13_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_13_END_STABILITY=8.5000
PRIMER_RIGHT_13_END_STABILITY=6.9000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_13=114
PRIMER_PAIR_PENALTY_14=0.1193
PRIMER_LEFT_14_PENALTY=0.036444
PRIMER_RIGHT_14_PENALTY=0.082838
PRIMER_LEFT_14_SEQUENCE=TTATATGCCTTTTGGAGCTGTGT
PRIMER_RIGHT_14_SEQUENCE=CCAAGATTTAAATAACCAAGGGC
PRIMER_LEFT_14=2216,23
PRIMER_RIGHT_14=3133,23
PRIMER_LEFT_14_TM=60.036
PRIMER_RIGHT_14_TM=60.083
PRIMER_LEFT_14_GC_PERCENT=39.130
PRIMER_RIGHT_14_GC_PERCENT=39.130
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=8.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=2.00
PRIMER_LEFT_14_MISPRIMING_SCORE=12.00, THE1BR THE-1B Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_RIGHT_14_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_14_MISPRIMING_SCORE=22.00, reverse L1MA2 3'-end of L1 repeat (subfamily L1MA2) - a consensus sequence
PRIMER_LEFT_14_END_STABILITY=6.4000
PRIMER_RIGHT_14_END_STABILITY=10.9000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_14=918
PRIMER_PAIR_PENALTY_15=0.1193
PRIMER_LEFT_15_PENALTY=0.036444
PRIMER_RIGHT_15_PENALTY=0.082838
PRIMER_LEFT_15_SEQUENCE=ATATGCCTTTTGGAGCTGTGTAA
PRIMER_RIGHT_15_SEQUENCE=CCAAGATTTAAATAACCAAGGGC
PRIMER_LEFT_15=2218,23
PRIMER_RIGHT_15=3133,23
PRIMER_LEFT_15_TM=60.036
PRIMER_RIGHT_15_TM=60.083
PRIMER_LEFT_15_GC_PERCENT=39.130
PRIMER_RIGHT_15_GC_PERCENT=39.130
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=8.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=2.00
PRIMER_LEFT_15_MISPRIMING_SCORE=12.00, THE1BR THE-1B Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_RIGHT_15_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_15_MISPRIMING_SCORE=22.00, reverse L1MA2 3'-end of L1 repeat (subfamily L1MA2) - a consensus sequence
PRIMER_LEFT_15_END_STABILITY=6.0000
PRIMER_RIGHT_15_END_STABILITY=10.9000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_15=916
PRIMER_PAIR_PENALTY_16=0.1193
PRIMER_LEFT_16_PENALTY=0.082838
PRIMER_RIGHT_16_PENALTY=0.036444
PRIMER_LEFT_16_SEQUENCE=GCCCTTGGTTATTTAAATCTTGG
PRIMER_RIGHT_16_SEQUENCE=TAAATGCCTGGCAAACAGTAGAT
PRIMER_LEFT_16=3111,23
PRIMER_RIGHT_16=4034,23
PRIMER_LEFT_16_TM=60.083
PRIMER_RIGHT_16_TM=60.036
PRIMER_LEFT_16_GC_PERCENT=39.130
PRIMER_RIGHT_16_GC_PERCENT=39.130
PRIMER_LEFT_16_SELF_ANY=8.00
PRIMER_RIGHT_16_SELF_ANY=7.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=2.00
PRIMER_LEFT_16_MISPRIMING_SCORE=12.00, reverse L1MA2 3'-end of L1 repeat (subfamily L1MA2) - a consensus sequence
PRIMER_RIGHT_16_MISPRIMING_SCORE=11.00, reverse MIR2 Repetitive element - a consensus
PRIMER_PAIR_16_MISPRIMING_SCORE=21.00, reverse L1MA2 3'-end of L1 repeat (subfamily L1MA2) - a consensus sequence
PRIMER_LEFT_16_END_STABILITY=8.5000
PRIMER_RIGHT_16_END_STABILITY=5.6000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_16=924
PRIMER_PAIR_PENALTY_17=0.1193
PRIMER_LEFT_17_PENALTY=0.082838
PRIMER_RIGHT_17_PENALTY=0.036444
PRIMER_LEFT_17_SEQUENCE=GCCCTTGGTTATTTAAATCTTGG
PRIMER_RIGHT_17_SEQUENCE=AAATGCCTGGCAAACAGTAGATA
PRIMER_LEFT_17=3111,23
PRIMER_RIGHT_17=4033,23
PRIMER_LEFT_17_TM=60.083
PRIMER_RIGHT_17_TM=60.036
PRIMER_LEFT_17_GC_PERCENT=39.130
PRIMER_RIGHT_17_GC_PERCENT=39.130
PRIMER_LEFT_17_SELF_ANY=8.00
PRIMER_RIGHT_17_SELF_ANY=7.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=2.00
PRIMER_LEFT_17_MISPRIMING_SCORE=12.00, reverse L1MA2 3'-end of L1 repeat (subfamily L1MA2) - a consensus sequence
PRIMER_RIGHT_17_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_17_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_17_END_STABILITY=8.5000
PRIMER_RIGHT_17_END_STABILITY=5.6000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_17=923
PRIMER_PAIR_PENALTY_18=0.1294
PRIMER_LEFT_18_PENALTY=0.014220
PRIMER_RIGHT_18_PENALTY=0.115144
PRIMER_LEFT_18_SEQUENCE=TTAGGCCACAATCTTCAATGAGT
PRIMER_RIGHT_18_SEQUENCE=TGTTTCTATGACCGTTGGAATCT
PRIMER_LEFT_18=169,23
PRIMER_RIGHT_18=1153,23
PRIMER_LEFT_18_TM=60.014
PRIMER_RIGHT_18_TM=59.885
PRIMER_LEFT_18_GC_PERCENT=39.130
PRIMER_RIGHT_18_GC_PERCENT=39.130
PRIMER_LEFT_18_SELF_ANY=5.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=3.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_18_MISPRIMING_SCORE=12.00, MER21B a consensus
PRIMER_PAIR_18_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_18_END_STABILITY=6.4000
PRIMER_RIGHT_18_END_STABILITY=6.6000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_18=985
PRIMER_PAIR_PENALTY_19=0.1294
PRIMER_LEFT_19_PENALTY=0.061028
PRIMER_RIGHT_19_PENALTY=0.068420
PRIMER_LEFT_19_SEQUENCE=ACTCCACCTTGTGTTTCAAAGAA
PRIMER_RIGHT_19_SEQUENCE=CTCTCTCAATAAACGCAAATGCT
PRIMER_LEFT_19=2999,23
PRIMER_RIGHT_19=3305,23
PRIMER_LEFT_19_TM=60.061
PRIMER_RIGHT_19_TM=59.932
PRIMER_LEFT_19_GC_PERCENT=39.130
PRIMER_RIGHT_19_GC_PERCENT=39.130
PRIMER_LEFT_19_SELF_ANY=5.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=3.00
PRIMER_RIGHT_19_SELF_END=2.00
PRIMER_LEFT_19_MISPRIMING_SCORE=12.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_19_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_19_MISPRIMING_SCORE=21.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_19_END_STABILITY=7.0000
PRIMER_RIGHT_19_END_STABILITY=8.1000
PRIMER_PAIR_19_COMPL_ANY=6.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_19=307
=