PRIMER_SEQUENCE_ID=NM_003625
SEQUENCE=GAGGCAAGTGAGGAGAGAAGATGCTGTAGCGTCCTCACCGGCTGCCAGCAGGGAAATGGTCCAGGAGTGCTGGGTGTGAGCCTCCCTTCTCCTCAAGCCGGAGACTGCGGTTGTCATTGATCAATTGAAGAAGCAAGGACCCGAAATCACAGACATTAGCAATGATGTGTGAAGTGATGCCCACGATTAATGAGGACACCCCAATGAGCCAAAGGGGGTCCCAAAGCAGTGGCTCGGACTCAGACTCCCATTTTGAGCAGCTGATGGTGAATATGCTAGATGAAAGGGATCGTCTTCTAGACACCCTTCGGGAGACCCAGGAAAGCCTCTCACTTGCCCAGCAAAGACTTCAGGATGTCATCTATGACCGAGACTCACTCCAGAGACAGCTCAATTCAGCCCTGCCACAGGATATCGAATCCCTAACAGGAGGGCTGGCTGGTTCTAAGGGGGCTGATCCACCGGAATTTGCTGCACTGACAAAAGAATTAAATGCCTGCAGGGAACAACTTCTAGAAAAGGAAGAAGAAATCTCTGAACTTAAAGCTGAAAGAAACAACACAAGACTATTACTGGAGCATTTGGAGTGCCTTGTGTCACGACATGAAAGATCACTAAGAATGACGGTGGTAAAACGGCAAGCCCAGTCTCCCTCAGGAGTATCCAGTGAAGTTGAAGTTCTCAAGGCACTGAAATCTTTGTTTGAGCACCACAAGGCCTTGGATGAAAAGGTAAGGGAGCGACTGAGGGTTTCTTTAGAAAGAGTCTCTGCACTGGAAGAAGAACTAGCTGCTGCTAATCAGGAGATTGTTGCCTTGCGTGAACAAAATGTTCATATACAAAGAAAAATGGCATCAAGCGAGGGATCCACAGAGTCAGAACATCTTGAAGGGATGGAACCTGGACAGAAAGTCCATGAGAAGCGTTTGTCCAATGGTTCTATAGACTCAACCGATGAAACTAGTCAAATAGTTGAACTACAAGAATTGCTTGAAAAGCAAAACTATGAAATGGCCCAGATGAAAGAACGTTTAGCAGCCCTTTCTTCCCGAGTGGGAGAGGTGGAACAGGAAGCAGAGACAGCAAGAAAGGATCTCATTAAAACAGAAGAAATGAACACCAAGTATCAAAGGGACATTAGGGAGGCCATGGCACAAAAGGAAGATATGGAAGAAAGAATTACAACCCTTGAAAAGCGTTACCTCAGTGCTCAGAGAGAATCTACCTCCATACATGACATGAATGATAAACTAGAAAATGAGTTAGCAAATAAAGAAGCTATCCTGCGGCAGATGGAAGAGAAAAACAGACAGTTACAAGAACGTCTTGAGCTAGCTGAACAAAAGTTGCAGCAGACCATGAGAAAGGCTGAAACCTTGCCTGAAGTAGAGGCTGAACTGGCTCAGAGAATTGCAGCCCTAACCAAGGCTGAAGAGAGACATGGAAATATTGAAGAACGTATGAGACATTTAGAGGGTCAACTTGAAGAGAAGAATCAAGAACTTCAAAGAGCTAGGCAAAGAGAGAAAATGAATGAGGAGCATAACAAGAGATTATCGGATACGGTTGATAGACTTCTGACTGAATCCAATGAACGCCTACAACTACACTTAAAGGAAAGAATGGCTGCTCTAGAAGAAAAGAATGTTTTAATTCAAGAATCAGAAACTTTCAGAAAGAATCTTGAAGAATCTTTACATGATAAGGAAAGATTAGCAGAAGAAATTGAAAAGCTGAGATCTGAACTTGACCAATTGAAAATGAGAACTGGCTCTTTAATTGAACCCACAATACCAAGAACTCATCTAGACACCTCAGCTGAGTTGCGGTACTCAGTGGGATCCCTAGTGGACAGCCAGTCTGATTACAGAACAACTAAAGTAATAAGAAGACCAAGGAGAGGCCGCATGGGTGTGCGAAGAGATGAGCCAAAGGTGAAATCTCTTGGGGATCACGAGTGGAATAGAACTCAACAGATTGGAGTACTAAGCAGCCACCCTTTTGAAAGTGACACTGAAATGTCTGATATTGATGATGATGACAGAGAAACAATTTTTAGCTCAATGGATCTTCTCTCTCCAAGTGGTCATTCCGATGCCCAGACGCTAGCCATGATGCTTCAGGAACAATTGGATGCCATCAACAAAGAAATCAGGCTAATTCAGGAAGAAAAAGAATCTACAGAGTTGCGTGCTGAAGAAATTGAAAATAGAGTGGCTAGTGTGAGCCTCGAAGGCCTGAATTTGGCAAGGGTCCACCCAGGTACCTCCATTACTGCCTCTGTTACAGCTTCATCGCTGGCCAGTTCATCTCCCCCCAGTGGACACTCAACTCCAAAGCTCACCCCTCGAAGCCCTGCCAGGGAAATGGATCGGATGGGAGTCATGACACTGCCAAGTGATCTGAGGAAACATCGGAGAAAGATTGCAGTTGTGGAAGAAGATGGTCGAGAGGACAAAGCAACAATTAAATGTGAAACTTCTCCTCCTCCTACCCCTAGAGCCCTCAGAATGACTCACACTCTCCCTTCTTCCTACCACAATGATGCTCGAAGTAGTTTATCTGTCTCTCTTGAGCCAGAAAGCCTCGGGCTTGGTAGTGCCAACAGCAGCCAAGACTCTCTTCACAAAGCCCCCAAGAAGAAAGGAATCAAGTCTTCAATAGGACGTTTGTTTGGTAAAAAAGAAAAAGCTCGACTTGGGCAGCTCCGAGGCTTTATGGAGACTGAAGCTGCAGCTCAGGAGTCCCTGGGGTTAGGCAAACTCGGAACTCAAGCTGAGAAGGATCGAAGACTAAAGAAAAAGCATGAACTTCTTGAAGAAGCTCGGAGAAAGGGATTACCTTTTGCCCAGTGGGATGGGCCAACTGTGGTCGCATGGCTAGAGCTTTGGTTGGGAATGCCTGCGTGGTACGTGGCAGCCTGCCGAGCCAACGTGAAGAGTGGTGCCATCATGTCTGCTTTATCTGACACTGAGATCCAGAGAGAAATTGGAATCAGCAATCCACTGCATCGCTTAAAACTTCGATTAGCAATCCAGGAGATGGTTTCCCTAACAAGTCCTTCAGCTCCTCCAACATCTCGAACTCCTTCAGGCAACGTTTGGGTGACTCATGAAGAAATGGAAAATCTTGCAGCTCCAGCAAAAACGAAAGAATCTGAGGAAGGAAGCTGGGCCCAGTGTCCGGTTTTTCTACAGACCCTGGCTTATGGAGATATGAATCATGAGTGGATTGGAAATGAATGGCTTCCCAGCTTGGGGTTACCTCAGTACAGAAGTTACTTTATGGAATGCTTGGTAGATGCAAGAATGTTAGATCACCTAACAAAAAAAGATCTCCGTGTCCATTTAAAAATGGTGGATAGTTTCCATCGAACAAGTTTACAATATGGAATTATGTGCTTAAAGAGGTTGAATTATGACAGAAAAGAACTAGAAAGAAGACGGGAAGCAAGCCAACATGAAATAAAAGACGTGTTGGTGTGGAGCAATGACCGAGTTATTCGCTGGATACAAGCAATTGGACTTCGAGAATATGCAAATAATATACTTGAGAGCGGTGTGCATGGCTCACTTATAGCCCTGGATGAAAACTTTGACTACAGCAGCTTAGCTTTATTATTACAGATTCCAACACAGAACACCCAGGCAAGGCAGATTCTTGAAAGAGAATACAATAACCTCTTGGCCCTGGGAACTGAAAGGCGACTGGATGAAAGTGATGACAAGAACTTCAGACGTGGATCAACCTGGAGAAGGCAGTTTCCTCCTCGTGAAGTACATGGAATCAGCATGATGCCTGGGTCCTCAGAAACATTACCAGCTGGATTTAGGTTAACCACAACCTCTGGGCAGTCAAGAAAAATGACAACAGATGTTGCTTCATCAAGACTGCAGAGGTTAGACAACTCCACTGTTCGCACATACTCATGTTGACCAGCCACTCAAAGGAGGCAGCACTGACCTGCTATGGCGTCTTTTCAGTCTACTCTACCTAAAGTGCACTACCATCTAAGAAGACGAGCAGTGAAAACCTTTGTGAAAACTGAATTC
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 46930, GC content failed 217, low tm 23366, high tm 12128, high any compl 4, high end compl 27, high repeat similarity 42, long poly-x seq 64, ok 11082
PRIMER_RIGHT_EXPLAIN=considered 46931, GC content failed 217, low tm 23761, high tm 11689, high any compl 1, high end compl 14, high repeat similarity 11, long poly-x seq 64, ok 11174
PRIMER_PAIR_EXPLAIN=considered 926, unacceptable product size 854, high any compl 1, high end compl 8, ok 63
PRIMER_PAIR_PENALTY=0.0284
PRIMER_LEFT_PENALTY=0.014220
PRIMER_RIGHT_PENALTY=0.014220
PRIMER_LEFT_SEQUENCE=CTGGATACAAGCAATTGGACTTC
PRIMER_RIGHT_SEQUENCE=AATCCAGCTGGTAATGTTTCTGA
PRIMER_LEFT=3505,23
PRIMER_RIGHT=3828,23
PRIMER_LEFT_TM=60.014
PRIMER_RIGHT_TM=60.014
PRIMER_LEFT_GC_PERCENT=43.478
PRIMER_RIGHT_GC_PERCENT=39.130
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=324
PRIMER_PAIR_PENALTY_1=0.0332
PRIMER_LEFT_1_PENALTY=0.018946
PRIMER_RIGHT_1_PENALTY=0.014220
PRIMER_LEFT_1_SEQUENCE=AAGCAAGCCAACATGAAATAAAA
PRIMER_RIGHT_1_SEQUENCE=AATCCAGCTGGTAATGTTTCTGA
PRIMER_LEFT_1=3447,23
PRIMER_RIGHT_1=3828,23
PRIMER_LEFT_1_TM=60.019
PRIMER_RIGHT_1_TM=60.014
PRIMER_LEFT_1_GC_PERCENT=30.435
PRIMER_RIGHT_1_GC_PERCENT=39.130
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_MISPRIMING_SCORE=12.00, L1MA2 3'-end of L1 repeat (subfamily L1MA2) - a consensus sequence
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_1_MISPRIMING_SCORE=23.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=382
PRIMER_PAIR_PENALTY_2=0.0547
PRIMER_LEFT_2_PENALTY=0.011619
PRIMER_RIGHT_2_PENALTY=0.043085
PRIMER_LEFT_2_SEQUENCE=CTCCTCGTGAAGTACATGGAATC
PRIMER_RIGHT_2_SEQUENCE=CACTGCTCGTCTTCTTAGATGGT
PRIMER_LEFT_2=3765,23
PRIMER_RIGHT_2=4027,23
PRIMER_LEFT_2_TM=60.012
PRIMER_RIGHT_2_TM=59.957
PRIMER_LEFT_2_GC_PERCENT=47.826
PRIMER_RIGHT_2_GC_PERCENT=47.826
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_2_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_2_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=7.8000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=263
PRIMER_PAIR_PENALTY_3=0.0554
PRIMER_LEFT_3_PENALTY=0.041227
PRIMER_RIGHT_3_PENALTY=0.014220
PRIMER_LEFT_3_SEQUENCE=ATGCTTGGTAGATGCAAGAATGT
PRIMER_RIGHT_3_SEQUENCE=AATCCAGCTGGTAATGTTTCTGA
PRIMER_LEFT_3=3289,23
PRIMER_RIGHT_3=3828,23
PRIMER_LEFT_3_TM=60.041
PRIMER_RIGHT_3_TM=60.014
PRIMER_LEFT_3_GC_PERCENT=39.130
PRIMER_RIGHT_3_GC_PERCENT=39.130
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_3_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=540
PRIMER_PAIR_PENALTY_4=0.0573
PRIMER_LEFT_4_PENALTY=0.014220
PRIMER_RIGHT_4_PENALTY=0.043085
PRIMER_LEFT_4_SEQUENCE=CTGGATACAAGCAATTGGACTTC
PRIMER_RIGHT_4_SEQUENCE=CACTGCTCGTCTTCTTAGATGGT
PRIMER_LEFT_4=3505,23
PRIMER_RIGHT_4=4027,23
PRIMER_LEFT_4_TM=60.014
PRIMER_RIGHT_4_TM=59.957
PRIMER_LEFT_4_GC_PERCENT=43.478
PRIMER_RIGHT_4_GC_PERCENT=47.826
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_4_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=7.8000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=523
PRIMER_PAIR_PENALTY_5=0.0612
PRIMER_LEFT_5_PENALTY=0.014220
PRIMER_RIGHT_5_PENALTY=0.046971
PRIMER_LEFT_5_SEQUENCE=CTGGATACAAGCAATTGGACTTC
PRIMER_RIGHT_5_SEQUENCE=CATGTACTTCACGAGGAGGAAAC
PRIMER_LEFT_5=3505,23
PRIMER_RIGHT_5=3782,23
PRIMER_LEFT_5_TM=60.014
PRIMER_RIGHT_5_TM=60.047
PRIMER_LEFT_5_GC_PERCENT=43.478
PRIMER_RIGHT_5_GC_PERCENT=47.826
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=2.00
PRIMER_RIGHT_5_SELF_END=2.00
PRIMER_LEFT_5_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_5_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_5_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_5_END_STABILITY=6.4000
PRIMER_RIGHT_5_END_STABILITY=6.7000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_5=278
PRIMER_PAIR_PENALTY_6=0.0659
PRIMER_LEFT_6_PENALTY=0.018946
PRIMER_RIGHT_6_PENALTY=0.046971
PRIMER_LEFT_6_SEQUENCE=AAGCAAGCCAACATGAAATAAAA
PRIMER_RIGHT_6_SEQUENCE=CATGTACTTCACGAGGAGGAAAC
PRIMER_LEFT_6=3447,23
PRIMER_RIGHT_6=3782,23
PRIMER_LEFT_6_TM=60.019
PRIMER_RIGHT_6_TM=60.047
PRIMER_LEFT_6_GC_PERCENT=30.435
PRIMER_RIGHT_6_GC_PERCENT=47.826
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=2.00
PRIMER_LEFT_6_MISPRIMING_SCORE=12.00, L1MA2 3'-end of L1 repeat (subfamily L1MA2) - a consensus sequence
PRIMER_RIGHT_6_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_6_MISPRIMING_SCORE=22.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_6_END_STABILITY=6.6000
PRIMER_RIGHT_6_END_STABILITY=6.7000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_6=336
PRIMER_PAIR_PENALTY_7=0.0708
PRIMER_LEFT_7_PENALTY=0.011619
PRIMER_RIGHT_7_PENALTY=0.059210
PRIMER_LEFT_7_SEQUENCE=CTCCTCGTGAAGTACATGGAATC
PRIMER_RIGHT_7_SEQUENCE=GGTTTTCACTGCTCGTCTTCTTA
PRIMER_LEFT_7=3765,23
PRIMER_RIGHT_7=4033,23
PRIMER_LEFT_7_TM=60.012
PRIMER_RIGHT_7_TM=59.941
PRIMER_LEFT_7_GC_PERCENT=47.826
PRIMER_RIGHT_7_GC_PERCENT=43.478
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=3.00
PRIMER_RIGHT_7_SELF_END=2.00
PRIMER_LEFT_7_MISPRIMING_SCORE=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_7_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_7_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_7_END_STABILITY=6.6000
PRIMER_RIGHT_7_END_STABILITY=6.0000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_7=269
PRIMER_PAIR_PENALTY_8=0.0734
PRIMER_LEFT_8_PENALTY=0.014220
PRIMER_RIGHT_8_PENALTY=0.059210
PRIMER_LEFT_8_SEQUENCE=CTGGATACAAGCAATTGGACTTC
PRIMER_RIGHT_8_SEQUENCE=GGTTTTCACTGCTCGTCTTCTTA
PRIMER_LEFT_8=3505,23
PRIMER_RIGHT_8=4033,23
PRIMER_LEFT_8_TM=60.014
PRIMER_RIGHT_8_TM=59.941
PRIMER_LEFT_8_GC_PERCENT=43.478
PRIMER_RIGHT_8_GC_PERCENT=43.478
PRIMER_LEFT_8_SELF_ANY=6.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=2.00
PRIMER_RIGHT_8_SELF_END=2.00
PRIMER_LEFT_8_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_8_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_8_END_STABILITY=6.4000
PRIMER_RIGHT_8_END_STABILITY=6.0000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_8=529
PRIMER_PAIR_PENALTY_9=0.0757
PRIMER_LEFT_9_PENALTY=0.028128
PRIMER_RIGHT_9_PENALTY=0.047559
PRIMER_LEFT_9_SEQUENCE=CGGATACGGTTGATAGACTTCTG
PRIMER_RIGHT_9_SEQUENCE=GTGGTAGGAAGAAGGGAGAGTGT
PRIMER_LEFT_9=1557,23
PRIMER_RIGHT_9=2539,23
PRIMER_LEFT_9_TM=60.028
PRIMER_RIGHT_9_TM=60.048
PRIMER_LEFT_9_GC_PERCENT=47.826
PRIMER_RIGHT_9_GC_PERCENT=52.174
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=2.00
PRIMER_LEFT_9_SELF_END=2.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_MISPRIMING_SCORE=12.00, reverse MER21 a consensus
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, reverse L1PA15 3'-end of L1 repeat (subfamily L1PA15) - a consensus sequence
PRIMER_PAIR_9_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_9_END_STABILITY=7.0000
PRIMER_RIGHT_9_END_STABILITY=6.1000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_9=983
PRIMER_PAIR_PENALTY_10=0.0757
PRIMER_LEFT_10_PENALTY=0.015214
PRIMER_RIGHT_10_PENALTY=0.060481
PRIMER_LEFT_10_SEQUENCE=AAAGGGATCGTCTTCTAGACACC
PRIMER_RIGHT_10_SEQUENCE=CGTTTTACCACCGTCATTCTTAG
PRIMER_LEFT_10=282,23
PRIMER_RIGHT_10=636,23
PRIMER_LEFT_10_TM=60.015
PRIMER_RIGHT_10_TM=59.940
PRIMER_LEFT_10_GC_PERCENT=47.826
PRIMER_RIGHT_10_GC_PERCENT=43.478
PRIMER_LEFT_10_SELF_ANY=6.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=3.00
PRIMER_RIGHT_10_SELF_END=3.00
PRIMER_LEFT_10_MISPRIMING_SCORE=12.00, reverse LTR13 LTR from a  putative human endogenous retrovirus
PRIMER_RIGHT_10_MISPRIMING_SCORE=11.00, MER21 a consensus
PRIMER_PAIR_10_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_10_END_STABILITY=7.6000
PRIMER_RIGHT_10_END_STABILITY=6.0000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_10=355
PRIMER_PAIR_PENALTY_11=0.0758
PRIMER_LEFT_11_PENALTY=0.061624
PRIMER_RIGHT_11_PENALTY=0.014220
PRIMER_LEFT_11_SEQUENCE=ATATACTTGAGAGCGGTGTGCAT
PRIMER_RIGHT_11_SEQUENCE=AATCCAGCTGGTAATGTTTCTGA
PRIMER_LEFT_11=3543,23
PRIMER_RIGHT_11=3828,23
PRIMER_LEFT_11_TM=60.062
PRIMER_RIGHT_11_TM=60.014
PRIMER_LEFT_11_GC_PERCENT=43.478
PRIMER_RIGHT_11_GC_PERCENT=39.130
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=8.00
PRIMER_LEFT_11_SELF_END=2.00
PRIMER_RIGHT_11_SELF_END=3.00
PRIMER_LEFT_11_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_11_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_11_END_STABILITY=8.4000
PRIMER_RIGHT_11_END_STABILITY=6.7000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_11=286
PRIMER_PAIR_PENALTY_12=0.0782
PRIMER_LEFT_12_PENALTY=0.018946
PRIMER_RIGHT_12_PENALTY=0.059210
PRIMER_LEFT_12_SEQUENCE=AAGCAAGCCAACATGAAATAAAA
PRIMER_RIGHT_12_SEQUENCE=GGTTTTCACTGCTCGTCTTCTTA
PRIMER_LEFT_12=3447,23
PRIMER_RIGHT_12=4033,23
PRIMER_LEFT_12_TM=60.019
PRIMER_RIGHT_12_TM=59.941
PRIMER_LEFT_12_GC_PERCENT=30.435
PRIMER_RIGHT_12_GC_PERCENT=43.478
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=2.00
PRIMER_LEFT_12_MISPRIMING_SCORE=12.00, L1MA2 3'-end of L1 repeat (subfamily L1MA2) - a consensus sequence
PRIMER_RIGHT_12_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_12_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_12_END_STABILITY=6.6000
PRIMER_RIGHT_12_END_STABILITY=6.0000
PRIMER_PAIR_12_COMPL_ANY=7.00
PRIMER_PAIR_12_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_12=587
PRIMER_PAIR_PENALTY_13=0.0802
PRIMER_LEFT_13_PENALTY=0.011619
PRIMER_RIGHT_13_PENALTY=0.068602
PRIMER_LEFT_13_SEQUENCE=CTCCTCGTGAAGTACATGGAATC
PRIMER_RIGHT_13_SEQUENCE=GTTTTCACAAAGGTTTTCACTGC
PRIMER_LEFT_13=3765,23
PRIMER_RIGHT_13=4044,23
PRIMER_LEFT_13_TM=60.012
PRIMER_RIGHT_13_TM=60.069
PRIMER_LEFT_13_GC_PERCENT=47.826
PRIMER_RIGHT_13_GC_PERCENT=39.130
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=3.00
PRIMER_RIGHT_13_SELF_END=2.00
PRIMER_LEFT_13_MISPRIMING_SCORE=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_13_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_13_MISPRIMING_SCORE=21.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_13_END_STABILITY=6.6000
PRIMER_RIGHT_13_END_STABILITY=7.9000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_13=280
PRIMER_PAIR_PENALTY_14=0.0828
PRIMER_LEFT_14_PENALTY=0.014220
PRIMER_RIGHT_14_PENALTY=0.068602
PRIMER_LEFT_14_SEQUENCE=CTGGATACAAGCAATTGGACTTC
PRIMER_RIGHT_14_SEQUENCE=GTTTTCACAAAGGTTTTCACTGC
PRIMER_LEFT_14=3505,23
PRIMER_RIGHT_14=4044,23
PRIMER_LEFT_14_TM=60.014
PRIMER_RIGHT_14_TM=60.069
PRIMER_LEFT_14_GC_PERCENT=43.478
PRIMER_RIGHT_14_GC_PERCENT=39.130
PRIMER_LEFT_14_SELF_ANY=6.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=2.00
PRIMER_RIGHT_14_SELF_END=2.00
PRIMER_LEFT_14_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_14_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_14_MISPRIMING_SCORE=24.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_14_END_STABILITY=6.4000
PRIMER_RIGHT_14_END_STABILITY=7.9000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_14=540
PRIMER_PAIR_PENALTY_15=0.0843
PRIMER_LEFT_15_PENALTY=0.041227
PRIMER_RIGHT_15_PENALTY=0.043085
PRIMER_LEFT_15_SEQUENCE=ATGCTTGGTAGATGCAAGAATGT
PRIMER_RIGHT_15_SEQUENCE=CACTGCTCGTCTTCTTAGATGGT
PRIMER_LEFT_15=3289,23
PRIMER_RIGHT_15=4027,23
PRIMER_LEFT_15_TM=60.041
PRIMER_RIGHT_15_TM=59.957
PRIMER_LEFT_15_GC_PERCENT=39.130
PRIMER_RIGHT_15_GC_PERCENT=47.826
PRIMER_LEFT_15_SELF_ANY=5.00
PRIMER_RIGHT_15_SELF_ANY=5.00
PRIMER_LEFT_15_SELF_END=2.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_15_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_15_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_15_END_STABILITY=6.6000
PRIMER_RIGHT_15_END_STABILITY=7.8000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_15=739
PRIMER_PAIR_PENALTY_16=0.0875
PRIMER_LEFT_16_PENALTY=0.018946
PRIMER_RIGHT_16_PENALTY=0.068602
PRIMER_LEFT_16_SEQUENCE=AAGCAAGCCAACATGAAATAAAA
PRIMER_RIGHT_16_SEQUENCE=GTTTTCACAAAGGTTTTCACTGC
PRIMER_LEFT_16=3447,23
PRIMER_RIGHT_16=4044,23
PRIMER_LEFT_16_TM=60.019
PRIMER_RIGHT_16_TM=60.069
PRIMER_LEFT_16_GC_PERCENT=30.435
PRIMER_RIGHT_16_GC_PERCENT=39.130
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=2.00
PRIMER_LEFT_16_MISPRIMING_SCORE=12.00, L1MA2 3'-end of L1 repeat (subfamily L1MA2) - a consensus sequence
PRIMER_RIGHT_16_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_16_MISPRIMING_SCORE=23.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_16_END_STABILITY=6.6000
PRIMER_RIGHT_16_END_STABILITY=7.9000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_16=598
PRIMER_PAIR_PENALTY_17=0.0882
PRIMER_LEFT_17_PENALTY=0.041227
PRIMER_RIGHT_17_PENALTY=0.046971
PRIMER_LEFT_17_SEQUENCE=ATGCTTGGTAGATGCAAGAATGT
PRIMER_RIGHT_17_SEQUENCE=CATGTACTTCACGAGGAGGAAAC
PRIMER_LEFT_17=3289,23
PRIMER_RIGHT_17=3782,23
PRIMER_LEFT_17_TM=60.041
PRIMER_RIGHT_17_TM=60.047
PRIMER_LEFT_17_GC_PERCENT=39.130
PRIMER_RIGHT_17_GC_PERCENT=47.826
PRIMER_LEFT_17_SELF_ANY=5.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=2.00
PRIMER_RIGHT_17_SELF_END=2.00
PRIMER_LEFT_17_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_17_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_17_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_17_END_STABILITY=6.6000
PRIMER_RIGHT_17_END_STABILITY=6.7000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_17=494
PRIMER_PAIR_PENALTY_18=0.0913
PRIMER_LEFT_18_PENALTY=0.005048
PRIMER_RIGHT_18_PENALTY=0.086226
PRIMER_LEFT_18_SEQUENCE=AAATGTGAAACTTCTCCTCCTCC
PRIMER_RIGHT_18_SEQUENCE=GCTTCCCGTCTTCTTTCTAGTTC
PRIMER_LEFT_18=2468,23
PRIMER_RIGHT_18=3450,23
PRIMER_LEFT_18_TM=59.995
PRIMER_RIGHT_18_TM=59.914
PRIMER_LEFT_18_GC_PERCENT=43.478
PRIMER_RIGHT_18_GC_PERCENT=47.826
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_18_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_18_MISPRIMING_SCORE=21.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_18_END_STABILITY=9.4000
PRIMER_RIGHT_18_END_STABILITY=6.4000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_18=983
PRIMER_PAIR_PENALTY_19=0.0975
PRIMER_LEFT_19_PENALTY=0.015214
PRIMER_RIGHT_19_PENALTY=0.082291
PRIMER_LEFT_19_SEQUENCE=AAAGGGATCGTCTTCTAGACACC
PRIMER_RIGHT_19_SEQUENCE=CCTCCCTAATGTCCCTTTGATAC
PRIMER_LEFT_19=282,23
PRIMER_RIGHT_19=1145,23
PRIMER_LEFT_19_TM=60.015
PRIMER_RIGHT_19_TM=60.082
PRIMER_LEFT_19_GC_PERCENT=47.826
PRIMER_RIGHT_19_GC_PERCENT=47.826
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=2.00
PRIMER_LEFT_19_SELF_END=3.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_MISPRIMING_SCORE=12.00, reverse LTR13 LTR from a  putative human endogenous retrovirus
PRIMER_RIGHT_19_MISPRIMING_SCORE=12.00, MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_PAIR_19_MISPRIMING_SCORE=20.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_19_END_STABILITY=7.6000
PRIMER_RIGHT_19_END_STABILITY=5.3000
PRIMER_PAIR_19_COMPL_ANY=7.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_19=864
=