PRIMER_SEQUENCE_ID=NM_003800
SEQUENCE=TACCAGTTTGAATACATGGCCCAGCACCTGGTGTTTGCCAACTGCATTCCTTTGATCCTAAAGTTCTTCAATCAAAACATCATGTCCTACATCACTGCCAAGAACAGCATTTCTGTCCTGAATGAAAGTGGCGCGCCGCCCCTGACGTTACCCGGATCGGAGAGGTTGGAATTCAGATTACGGCTGCGATTCGGGTGTCTCGGACCCCGGTGTGCACCGGACCACGGGGAGGCGGCTCCAAAGGCGCGGTGAACGTTGGTGAGGGAGGGCAGCTCTGCGCCCCAAGAGATGGCTCACAACAAGATCCCGCCGCGGTGGCTGAACTGTCCCCGGCGCGGCCAGCCGGTGGCAGGAAGATTCTTACCTCTGAAGACAATATTAGGACCAAGATATGATAGTCAAGTTGCTGAAGAAAATCGGTTCCATCCCAGCATGCTCTCAAATTACCTAAAGAGCCTAAAGGTTAAAATGGGCTTGTTGGTGGACCTGACAAATACTTCAAGGTTCTATGACCGAAATGACATAGAAAAAGAAGGAATCAAATATATAAAACTTCAGTGTAAAGGACATGGTGAGTGCCCTACCACTGAGAATACTGAGACCTTTATTCGTCTGTGTGAGCGGTTTAATGAAAGAAATCCGCCTGAACTTATAGGTGTTCATTGTACTCATGGCTTCAATCGCACTGGTTTCCTCATATGTGCCTTTTTGGTGGAGAAAATGGATTGGAGTATCGAAGCAGCAGTTGCTACTTTTGCCCAAGCCAGACCACCAGGAATCTACAAGGGTGATTATTTGAAGGAACTTTTTCGTCGGTATGGTGACATAGAGGAAGCACCACCCCCACCTCTATTGCCAGATTGGTGTTTTGAGGATGATGAAGACGAAGATGAGGATGAGGATGGAAAGAAGGAATCAGAACCCGGGTCAAGTGCTTCTTTTGGCAAAAGGAGAAAAGAACGGTTAAAACTGGGCGCTATTTTCTTGGAAGGTGTTACTGTTAAAGGTGTAACTCAAGTAACAACACAACCAAAGTTAGGAGAGGTACAGCAGAAGTGTCATCAATTCTGTGGCTGGGAAGGGTCTGGATTCCCTGGAGCACAGCCTGTTTCCATGGACAAGCAAAATATTAAACTTTTAGACCTGAAGCCATACAAAGTAAGCTGGAAAGCAGATGGTACTCGGTACATGATGTTGATTGATGGCACAAATGAAGTTTTTATGATTGATAGAGACAATTCAGTATTTCATGTTTCAAATCTGGAATTTCCATTTCGTAAAGATCTTCGTATGCATTTATCAAATACTCTCTTGGATGGCGAGATGATTATTGACAGAGTAAATGGACAGGCTGTTCCTAGATATTTGATATATGACATAATTAAATTCAATTCACAGCCCGTTGGAGATTGTGATTTTAATGTTCGTCTGCAGTGTATAGAACGAGAAATTATAAGTCCTCGACACGAAAAAATGAAGACTGGGCTCATTGACAAAACACAGGAACCATTTAGCGTCAGAAATAAGCCGTTTTTTGACATCTGTACTTCAAGAAAGCTACTTGAAGGAAATTTTGCCAAAGAAGTGAGCCATGAAATGGATGGACTTATTTTTCAGCCTACTGGAAAATACAAACCTGGTCGATGTGATGATATTTTGAAATGGAAGCCTCCCAGTCTGAATTCTGTGGATTTTCGTCTAAAAATAACCAGAATGGGAGGAGAAGGGTTACTTCCTCCGAATGTTGGCCTCCTGTATGTTGGAGGTTATGAAAGACCCTTTGCACAAATCAAGGTGACAAAAGAGCTGAAACAGTATGACAACAAAATTATAGAATGCAAATTTGAGAACAACAGCTGGGTCTTCATGAGACAGAGAACAGACAAAAGTTTTCCTAATGCCTACAACACTGCCATGGCTGTGTGTAACAGCATCTCAAACCCTGTCACCAAGGAGATGCTGTTTGAGTTCATCGACAGATGTACTGCAGCTTCTCAAGGACAGAAGCGAAAACATCATCTGGACCCTGACACGGAGCTCATGCCACCACCACCTCCCAAAAGACCACGCCCTTTAACCTAAGACCTGCCTGTGACTTGAGGGTTAAGAAGAAAGAGGAATGAGGAAAAAACGCTGTTGCCCATTTTTGTCGCCGAGAAATTGAAAAACGACTGTGGCTTGATGTTGATACACATTTGAAATTTTTTTTAAAAAAAAAGAATTATCGTAGCCAGCCTGTAAATACTTGATGCATTCGTTTCCTCAGTGCTGCAATACATCATGGACTTAAACATCTTATTTATCTGATAAACTCAGCCTTACCTTGTGGAATCTGAAGATTGAAATATCCAAAGGTTTAAACAAGATGGAAATCAATGAAAAAGAGGCCTTGTTTATATATGGGTAGCTTCACATTTAATTTATAAAGTTAACAATCTGGAAACTGTGAGCCCATAAAACAGTATTTTTTAGAAGTTGTGTTACAACTGTAGCAAATTTTCCTTTTAAAAAATCCAGGCAATGGCATTAAACATTCTTGCTATCATTTACCATGGATTTCCTACATTTCTGAGATCCCTGTATTCAAAAACAAATGACAGGGTTGTTAAGTTGTACTATATTAAACAACTTGGTCTGGTTTATATCATTTTAACAAGTTTTTTGGAAATGTAAAAGAAAAAAGTAAAGCTCCATTTTTGTGAATTGCATTGTTTCTAAAGGAAGTATTTTGAGTATTTTCATCTGTTTGTATTGTTTCGACTATGATATCAGATATCCAGTGTGTCCATTCTGGCAGTGGTTTTGAGTTACTTCATGGAACTTGGACATACACCACCAGGTCCTTTATGTGTTGTTCTCCTGGGAGACTTCTTTTTTTAAATGCCAGGCTACTTCTGCTTTACCTCAGTGGTATCAGCACATTGTTAATTACGTTAAAACACACAACTCACTGTGATCTATGGGAGTGATATCAAATACAAACGATGTTGTGTTCCTTCTCTCGAACAAAACAGCAAAGGGAATGCCAGCTAACTTCTTTGATTAGAAGGTAATGTCATGGAATAGAATGCTGTCACCAGAAAATGCTCTCCCACTGCTAGATAAATGCAGAGGCAAAGTGTAGACATCTGTAGGAACTAAATATCAAGGAAATCAAGACATTTATTTTCAAAGCGAGAATTCTATCTACATTCTATTCTGCGGATGTTATTTCTGTTATACATCTGAATTTTAAAATGTTTACTGATAGATAATGGATTTAAAGGGCGGCTAATTGATTGTCTGCCATTGTTGATAAACTGTTCTAATTGGTTTGTGAAAGAACTCTGAGAATTAGCTAAATGGAGGCTAGTGTATTTATTTTTAGAGGTACATGTTTTAAGTAAATGTATTTAAAACAAATTTTCATGGTCTGAATTGTATATGTAAATGCATATCTGTGTAAGTATATGCAAGTGTATATTCTTTCTACTGATTTCAGTCAGACACGAAATCCAACTGAATTACCCGGTAGACTGAATATCTGACGGGATTTCTATATGGACATAGCACAGATGAACCAAAAATGTATTTATATTCACCTGAGTTTTAATGTTTTTAAACAAAGTTTTCTCCTGCAGTGCTTTTCTATATGAAATAGTTGAGTCAAGCTTGGGCTTTTTTCTTCGTTGTTCCCAAGTCATGTAATGTATAGTGAATTTATCCAAAACGCCATTGTAACGTTTTTTACTAGAGTGTTTTTGCACATTTGGGCGCTAAAGGGATTAAAACAACTGATGCCACAATGACTATTGAAGGAGGGAAAATTCTCAAGGTTCCATAGTATCTTTACAAAATGGATTAAAAAAAAATGCCTATGCAACTTCAAATAGTCTGATGCCTGCTTCCCCAGGACGAGTGACTCTTCCACGGTGTGCTGCTTTCCCTCCGAGCCTGTGGAAAGGCAGAGCCCTTTTGCAGATACGAAAGCAAGCTTTTGCCAATGATCTTTAATGGAGTTAAAATGTTTATGGTAATTGTAACCTTTCAAATGAGTTACGTGAAAAGAGCATCTCACTTTTAATACACCCAGATATTTTCTTCAAGTCTTTGCGCATTTTTAGCAGGACTTAATTTTCCTAAATTTATTCTTTCCTTTCAATTATAGTGACATAGAGTAGTTGGCCTTATAAGTAGATCCCATTTAGCCCCTGAACATAATACCATCTGCAGTATTATAAAAGTCATTTAGAAGGTCAGGGGGATAATCTAGAGGCAGGATTTTGGACATTGTGAAGGAAATGTGCTCCTTCTCAGCTCACTTCAATAACTATTTTCTGAGACTGAAGTTTTTTAGACAAGAATAAGAAAACTTTGCTTTCTTCAGTTATCACATGTGAAAGCTTTTGCTTTTTGTTAGGAAAAGGTGTGGATGACTCTACTGCGTGGATGGTTACATTTGCTTTCCGTGAAATGCTCAGAAAATGTCAGGACATTCCTTTTCCAATTGTGTGTCAGTGCGTATTGTAATAAAACTACTGATGGAATTCCGGAAAAAAAAAAAAAA
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 51452, GC content failed 2546, low tm 29043, high tm 8821, high any compl 9, high end compl 30, high repeat similarity 42, long poly-x seq 258, ok 10703
PRIMER_RIGHT_EXPLAIN=considered 51412, GC content failed 2613, low tm 29111, high tm 8658, high any compl 4, high end compl 11, high repeat similarity 12, long poly-x seq 271, ok 10732
PRIMER_PAIR_EXPLAIN=considered 1163, unacceptable product size 1097, high end compl 4, ok 62
PRIMER_PAIR_PENALTY=0.0104
PRIMER_LEFT_PENALTY=0.000796
PRIMER_RIGHT_PENALTY=0.009608
PRIMER_LEFT_SEQUENCE=GAAGGAAATTTTGCCAAAGAAGT
PRIMER_RIGHT_SEQUENCE=TCAATCTTCAGATTCCACAAGGT
PRIMER_LEFT=1563,23
PRIMER_RIGHT=2342,23
PRIMER_LEFT_TM=60.001
PRIMER_RIGHT_TM=59.990
PRIMER_LEFT_GC_PERCENT=34.783
PRIMER_RIGHT_GC_PERCENT=39.130
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=780
PRIMER_PAIR_PENALTY_1=0.0189
PRIMER_LEFT_1_PENALTY=0.009302
PRIMER_RIGHT_1_PENALTY=0.009608
PRIMER_LEFT_1_SEQUENCE=AAAAAGAATTATCGTAGCCAGCC
PRIMER_RIGHT_1_SEQUENCE=TCAATCTTCAGATTCCACAAGGT
PRIMER_LEFT_1=2213,23
PRIMER_RIGHT_1=2342,23
PRIMER_LEFT_1_TM=60.009
PRIMER_RIGHT_1_TM=59.990
PRIMER_LEFT_1_GC_PERCENT=39.130
PRIMER_RIGHT_1_GC_PERCENT=39.130
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_1_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_1_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=130
PRIMER_PAIR_PENALTY_2=0.0261
PRIMER_LEFT_2_PENALTY=0.010529
PRIMER_RIGHT_2_PENALTY=0.015613
PRIMER_LEFT_2_SEQUENCE=CTGATGCCACAATGACTATTGAA
PRIMER_RIGHT_2_SEQUENCE=GTCCAAAATCCTGCCTCTAGATT
PRIMER_LEFT_2=3773,23
PRIMER_RIGHT_2=4267,23
PRIMER_LEFT_2_TM=60.011
PRIMER_RIGHT_2_TM=59.984
PRIMER_LEFT_2_GC_PERCENT=39.130
PRIMER_RIGHT_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_2_MISPRIMING_SCORE=12.00, reverse MLT2C2 Interspersed repeat MLT2C2 - a consensus
PRIMER_PAIR_2_MISPRIMING_SCORE=20.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=495
PRIMER_PAIR_PENALTY_3=0.0374
PRIMER_LEFT_3_PENALTY=0.000796
PRIMER_RIGHT_3_PENALTY=0.036623
PRIMER_LEFT_3_SEQUENCE=GAAGGAAATTTTGCCAAAGAAGT
PRIMER_RIGHT_3_SEQUENCE=GTCCTTGAGAAGCTGCAGTACAT
PRIMER_LEFT_3=1563,23
PRIMER_RIGHT_3=2001,23
PRIMER_LEFT_3_TM=60.001
PRIMER_RIGHT_3_TM=59.963
PRIMER_LEFT_3_GC_PERCENT=34.783
PRIMER_RIGHT_3_GC_PERCENT=47.826
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_3_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=5.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=439
PRIMER_PAIR_PENALTY_4=0.0459
PRIMER_LEFT_4_PENALTY=0.009302
PRIMER_RIGHT_4_PENALTY=0.036623
PRIMER_LEFT_4_SEQUENCE=AAAAAGAATTATCGTAGCCAGCC
PRIMER_RIGHT_4_SEQUENCE=AGATGTCTACACTTTGCCTCTGC
PRIMER_LEFT_4=2213,23
PRIMER_RIGHT_4=3129,23
PRIMER_LEFT_4_TM=60.009
PRIMER_RIGHT_4_TM=59.963
PRIMER_LEFT_4_GC_PERCENT=39.130
PRIMER_RIGHT_4_GC_PERCENT=47.826
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, reverse MSTAR MSTa-Mammalian LTR internal retrotransposon sequence - a consensus
PRIMER_PAIR_4_MISPRIMING_SCORE=21.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=917
PRIMER_PAIR_PENALTY_5=0.0536
PRIMER_LEFT_5_PENALTY=0.043085
PRIMER_RIGHT_5_PENALTY=0.010529
PRIMER_LEFT_5_SEQUENCE=ACAAACGATGTTGTGTTCCTTCT
PRIMER_RIGHT_5_SEQUENCE=TTCAATAGTCATTGTGGCATCAG
PRIMER_LEFT_5=2977,23
PRIMER_RIGHT_5=3795,23
PRIMER_LEFT_5_TM=59.957
PRIMER_RIGHT_5_TM=60.011
PRIMER_LEFT_5_GC_PERCENT=39.130
PRIMER_RIGHT_5_GC_PERCENT=39.130
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=5.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_5_MISPRIMING_SCORE=12.00, MER21B a consensus
PRIMER_PAIR_5_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_5_END_STABILITY=6.7000
PRIMER_RIGHT_5_END_STABILITY=6.6000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_5=819
PRIMER_PAIR_PENALTY_6=0.0536
PRIMER_LEFT_6_PENALTY=0.043085
PRIMER_RIGHT_6_PENALTY=0.010529
PRIMER_LEFT_6_SEQUENCE=ACAAACGATGTTGTGTTCCTTCT
PRIMER_RIGHT_6_SEQUENCE=CTTCAATAGTCATTGTGGCATCA
PRIMER_LEFT_6=2977,23
PRIMER_RIGHT_6=3796,23
PRIMER_LEFT_6_TM=59.957
PRIMER_RIGHT_6_TM=60.011
PRIMER_LEFT_6_GC_PERCENT=39.130
PRIMER_RIGHT_6_GC_PERCENT=39.130
PRIMER_LEFT_6_SELF_ANY=6.00
PRIMER_RIGHT_6_SELF_ANY=5.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=3.00
PRIMER_LEFT_6_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_6_MISPRIMING_SCORE=11.00, MER21B a consensus
PRIMER_PAIR_6_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_6_END_STABILITY=6.7000
PRIMER_RIGHT_6_END_STABILITY=6.9000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_6=820
PRIMER_PAIR_PENALTY_7=0.0542
PRIMER_LEFT_7_PENALTY=0.044904
PRIMER_RIGHT_7_PENALTY=0.009302
PRIMER_LEFT_7_SEQUENCE=TTCGTCTGCAGTGTATAGAACGA
PRIMER_RIGHT_7_SEQUENCE=GGCTGGCTACGATAATTCTTTTT
PRIMER_LEFT_7=1423,23
PRIMER_RIGHT_7=2235,23
PRIMER_LEFT_7_TM=59.955
PRIMER_RIGHT_7_TM=60.009
PRIMER_LEFT_7_GC_PERCENT=43.478
PRIMER_RIGHT_7_GC_PERCENT=39.130
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=3.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_7_MISPRIMING_SCORE=12.00, MER7B Nonautonomous DNA transposon
PRIMER_PAIR_7_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_7_END_STABILITY=8.4000
PRIMER_RIGHT_7_END_STABILITY=7.6000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_7=813
PRIMER_PAIR_PENALTY_8=0.0545
PRIMER_LEFT_8_PENALTY=0.044904
PRIMER_RIGHT_8_PENALTY=0.009608
PRIMER_LEFT_8_SEQUENCE=TTCGTCTGCAGTGTATAGAACGA
PRIMER_RIGHT_8_SEQUENCE=TCAATCTTCAGATTCCACAAGGT
PRIMER_LEFT_8=1423,23
PRIMER_RIGHT_8=2342,23
PRIMER_LEFT_8_TM=59.955
PRIMER_RIGHT_8_TM=59.990
PRIMER_LEFT_8_GC_PERCENT=43.478
PRIMER_RIGHT_8_GC_PERCENT=39.130
PRIMER_LEFT_8_SELF_ANY=6.00
PRIMER_RIGHT_8_SELF_ANY=8.00
PRIMER_LEFT_8_SELF_END=3.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_8_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_8_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_8_END_STABILITY=8.4000
PRIMER_RIGHT_8_END_STABILITY=7.9000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_8=920
PRIMER_PAIR_PENALTY_9=0.0577
PRIMER_LEFT_9_PENALTY=0.009302
PRIMER_RIGHT_9_PENALTY=0.048411
PRIMER_LEFT_9_SEQUENCE=AAAAAGAATTATCGTAGCCAGCC
PRIMER_RIGHT_9_SEQUENCE=TAACTCAAAACCACTGCCAGAAT
PRIMER_LEFT_9=2213,23
PRIMER_RIGHT_9=2809,23
PRIMER_LEFT_9_TM=60.009
PRIMER_RIGHT_9_TM=60.048
PRIMER_LEFT_9_GC_PERCENT=39.130
PRIMER_RIGHT_9_GC_PERCENT=39.130
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=5.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=2.00
PRIMER_LEFT_9_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, reverse L1PA2 3'-end of L1 repeat (subfamily L1PA2) - a consensus sequence
PRIMER_PAIR_9_MISPRIMING_SCORE=24.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_9_END_STABILITY=9.7000
PRIMER_RIGHT_9_END_STABILITY=6.6000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_9=597
PRIMER_PAIR_PENALTY_10=0.0641
PRIMER_LEFT_10_PENALTY=0.000796
PRIMER_RIGHT_10_PENALTY=0.063281
PRIMER_LEFT_10_SEQUENCE=GAAGGAAATTTTGCCAAAGAAGT
PRIMER_RIGHT_10_SEQUENCE=ACAGGCAGGTCTTAGGTTAAAGG
PRIMER_LEFT_10=1563,23
PRIMER_RIGHT_10=2092,23
PRIMER_LEFT_10_TM=60.001
PRIMER_RIGHT_10_TM=60.063
PRIMER_LEFT_10_GC_PERCENT=34.783
PRIMER_RIGHT_10_GC_PERCENT=47.826
PRIMER_LEFT_10_SELF_ANY=7.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=3.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_10_MISPRIMING_SCORE=10.00, L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence
PRIMER_PAIR_10_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_10_END_STABILITY=6.4000
PRIMER_RIGHT_10_END_STABILITY=8.5000
PRIMER_PAIR_10_COMPL_ANY=6.00
PRIMER_PAIR_10_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_10=530
PRIMER_PAIR_PENALTY_11=0.0726
PRIMER_LEFT_11_PENALTY=0.063281
PRIMER_RIGHT_11_PENALTY=0.009302
PRIMER_LEFT_11_SEQUENCE=CCTTTAACCTAAGACCTGCCTGT
PRIMER_RIGHT_11_SEQUENCE=GGCTGGCTACGATAATTCTTTTT
PRIMER_LEFT_11=2070,23
PRIMER_RIGHT_11=2235,23
PRIMER_LEFT_11_TM=60.063
PRIMER_RIGHT_11_TM=60.009
PRIMER_LEFT_11_GC_PERCENT=47.826
PRIMER_RIGHT_11_GC_PERCENT=39.130
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=2.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_MISPRIMING_SCORE=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, MER7B Nonautonomous DNA transposon
PRIMER_PAIR_11_MISPRIMING_SCORE=22.00, MER7B Nonautonomous DNA transposon
PRIMER_LEFT_11_END_STABILITY=7.9000
PRIMER_RIGHT_11_END_STABILITY=7.6000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_11=166
PRIMER_PAIR_PENALTY_12=0.0729
PRIMER_LEFT_12_PENALTY=0.063281
PRIMER_RIGHT_12_PENALTY=0.009608
PRIMER_LEFT_12_SEQUENCE=CCTTTAACCTAAGACCTGCCTGT
PRIMER_RIGHT_12_SEQUENCE=TCAATCTTCAGATTCCACAAGGT
PRIMER_LEFT_12=2070,23
PRIMER_RIGHT_12=2342,23
PRIMER_LEFT_12_TM=60.063
PRIMER_RIGHT_12_TM=59.990
PRIMER_LEFT_12_GC_PERCENT=47.826
PRIMER_RIGHT_12_GC_PERCENT=39.130
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=8.00
PRIMER_LEFT_12_SELF_END=2.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_MISPRIMING_SCORE=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_12_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_12_MISPRIMING_SCORE=22.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_12_END_STABILITY=7.9000
PRIMER_RIGHT_12_END_STABILITY=7.9000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_12=273
PRIMER_PAIR_PENALTY_13=0.0797
PRIMER_LEFT_13_PENALTY=0.043085
PRIMER_RIGHT_13_PENALTY=0.036623
PRIMER_LEFT_13_SEQUENCE=ACAAACGATGTTGTGTTCCTTCT
PRIMER_RIGHT_13_SEQUENCE=AGATGTCTACACTTTGCCTCTGC
PRIMER_LEFT_13=2977,23
PRIMER_RIGHT_13=3129,23
PRIMER_LEFT_13_TM=59.957
PRIMER_RIGHT_13_TM=59.963
PRIMER_LEFT_13_GC_PERCENT=39.130
PRIMER_RIGHT_13_GC_PERCENT=47.826
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=2.00
PRIMER_LEFT_13_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_13_MISPRIMING_SCORE=12.00, reverse MSTAR MSTa-Mammalian LTR internal retrotransposon sequence - a consensus
PRIMER_PAIR_13_MISPRIMING_SCORE=20.00, reverse L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence
PRIMER_LEFT_13_END_STABILITY=6.7000
PRIMER_RIGHT_13_END_STABILITY=8.2000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_13=153
PRIMER_PAIR_PENALTY_14=0.0815
PRIMER_LEFT_14_PENALTY=0.044904
PRIMER_RIGHT_14_PENALTY=0.036623
PRIMER_LEFT_14_SEQUENCE=TTCGTCTGCAGTGTATAGAACGA
PRIMER_RIGHT_14_SEQUENCE=GTCCTTGAGAAGCTGCAGTACAT
PRIMER_LEFT_14=1423,23
PRIMER_RIGHT_14=2001,23
PRIMER_LEFT_14_TM=59.955
PRIMER_RIGHT_14_TM=59.963
PRIMER_LEFT_14_GC_PERCENT=43.478
PRIMER_RIGHT_14_GC_PERCENT=47.826
PRIMER_LEFT_14_SELF_ANY=6.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=3.00
PRIMER_RIGHT_14_SELF_END=2.00
PRIMER_LEFT_14_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_14_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_14_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_14_END_STABILITY=8.4000
PRIMER_RIGHT_14_END_STABILITY=5.6000
PRIMER_PAIR_14_COMPL_ANY=6.00
PRIMER_PAIR_14_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_14=579
PRIMER_PAIR_PENALTY_15=0.0855
PRIMER_LEFT_15_PENALTY=0.012354
PRIMER_RIGHT_15_PENALTY=0.073123
PRIMER_LEFT_15_SEQUENCE=GAAAGAAATCCGCCTGAACTTAT
PRIMER_RIGHT_15_SEQUENCE=CCCAGTTTTAACCGTTCTTTTCT
PRIMER_LEFT_15=630,23
PRIMER_RIGHT_15=973,23
PRIMER_LEFT_15_TM=59.988
PRIMER_RIGHT_15_TM=59.927
PRIMER_LEFT_15_GC_PERCENT=39.130
PRIMER_RIGHT_15_GC_PERCENT=39.130
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=5.00
PRIMER_LEFT_15_SELF_END=2.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_MISPRIMING_SCORE=11.00, Tigger2 Autonomous DNA transposon
PRIMER_RIGHT_15_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_15_MISPRIMING_SCORE=21.00, Tigger2 Autonomous DNA transposon
PRIMER_LEFT_15_END_STABILITY=5.9000
PRIMER_RIGHT_15_END_STABILITY=7.0000
PRIMER_PAIR_15_COMPL_ANY=6.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_15=344
PRIMER_PAIR_PENALTY_16=0.0979
PRIMER_LEFT_16_PENALTY=0.088604
PRIMER_RIGHT_16_PENALTY=0.009302
PRIMER_LEFT_16_SEQUENCE=AGATGTACTGCAGCTTCTCAAGG
PRIMER_RIGHT_16_SEQUENCE=GGCTGGCTACGATAATTCTTTTT
PRIMER_LEFT_16=1977,23
PRIMER_RIGHT_16=2235,23
PRIMER_LEFT_16_TM=60.089
PRIMER_RIGHT_16_TM=60.009
PRIMER_LEFT_16_GC_PERCENT=47.826
PRIMER_RIGHT_16_GC_PERCENT=39.130
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_16_MISPRIMING_SCORE=12.00, MER7B Nonautonomous DNA transposon
PRIMER_PAIR_16_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_16_END_STABILITY=8.5000
PRIMER_RIGHT_16_END_STABILITY=7.6000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_16=259
PRIMER_PAIR_PENALTY_17=0.0982
PRIMER_LEFT_17_PENALTY=0.088604
PRIMER_RIGHT_17_PENALTY=0.009608
PRIMER_LEFT_17_SEQUENCE=AGATGTACTGCAGCTTCTCAAGG
PRIMER_RIGHT_17_SEQUENCE=TCAATCTTCAGATTCCACAAGGT
PRIMER_LEFT_17=1977,23
PRIMER_RIGHT_17=2342,23
PRIMER_LEFT_17_TM=60.089
PRIMER_RIGHT_17_TM=59.990
PRIMER_LEFT_17_GC_PERCENT=47.826
PRIMER_RIGHT_17_GC_PERCENT=39.130
PRIMER_LEFT_17_SELF_ANY=6.00
PRIMER_RIGHT_17_SELF_ANY=8.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_17_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_17_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_17_END_STABILITY=8.5000
PRIMER_RIGHT_17_END_STABILITY=7.9000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_17=366
PRIMER_PAIR_PENALTY_18=0.1082
PRIMER_LEFT_18_PENALTY=0.044904
PRIMER_RIGHT_18_PENALTY=0.063281
PRIMER_LEFT_18_SEQUENCE=TTCGTCTGCAGTGTATAGAACGA
PRIMER_RIGHT_18_SEQUENCE=ACAGGCAGGTCTTAGGTTAAAGG
PRIMER_LEFT_18=1423,23
PRIMER_RIGHT_18=2092,23
PRIMER_LEFT_18_TM=59.955
PRIMER_RIGHT_18_TM=60.063
PRIMER_LEFT_18_GC_PERCENT=43.478
PRIMER_RIGHT_18_GC_PERCENT=47.826
PRIMER_LEFT_18_SELF_ANY=6.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=3.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_18_MISPRIMING_SCORE=10.00, L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence
PRIMER_PAIR_18_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_18_END_STABILITY=8.4000
PRIMER_RIGHT_18_END_STABILITY=8.5000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_18=670
PRIMER_PAIR_PENALTY_19=0.1085
PRIMER_LEFT_19_PENALTY=0.043085
PRIMER_RIGHT_19_PENALTY=0.065377
PRIMER_LEFT_19_SEQUENCE=ACAAACGATGTTGTGTTCCTTCT
PRIMER_RIGHT_19_SEQUENCE=CAGTCTACCGGGTAATTCAGTTG
PRIMER_LEFT_19=2977,23
PRIMER_RIGHT_19=3517,23
PRIMER_LEFT_19_TM=59.957
PRIMER_RIGHT_19_TM=59.935
PRIMER_LEFT_19_GC_PERCENT=39.130
PRIMER_RIGHT_19_GC_PERCENT=47.826
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=7.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=2.00
PRIMER_LEFT_19_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_19_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_19_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_19_END_STABILITY=6.7000
PRIMER_RIGHT_19_END_STABILITY=6.7000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_19=541
=