PRIMER_SEQUENCE_ID=NM_003828
SEQUENCE=AGCGGGGGGGTAACGCGGGGCCGCCAGGTGACAGCTAATGGCGGCGGCCGCCTGAGGCGGGCGGGCGGTATAGAGCGGGCGGCAGGAGGCAAGCAGCGAAACCTTCCCGGCCGCCGCTCCCGTCCCGACGGCGGCTTCCCCAAGGCGGCAGGACTCGGCGCGCCATGGACAGGCCGGCGGCGGCGGCGGCGGCGGGCTGCGAGGGCGGCGGGGGCCCGAACCCGGGGCCGGCGGGCGGCAGGAGGCCTCCTCGGGCCGCGGGGGGCGCCACCGCCGGCTCCCGGCAGCCCAGCGTGGAGACCCTGGACAGTCCCACAGGATCACATGTTGAATGGTGTAAACAGCTTATAGCTGCTACAATTTCTAGTCAGATTTCAGGTTCAGTGACATCAGAAAATGTGTCCAGAGATTACAAGGCTCTAAGGGATGGAAATAAGCTGGCACAGATGGAAGAGGCTCCACTTTTCCCAGGAGAATCAATTAAAGCCATTGTGAAAGATGTCATGTATATCTGCCCATTTATGGGAGCAGTGAGTGGAACCCTGACAGTGACGGACTTTAAGCTGTACTTCAAAAATGTCGAGAGGGACCCGCATTTTATCCTTGATGTTCCCCTTGGAGTGATCAGCAGAGTGGAGAAGATTGGAGCACAGAGCCATGGAGACAATTCCTGTGGTATAGAGATAGTGTGCAAGGATATGAGGAACTTGCGGCTTGCTTATAAACAGGAAGAACAGAGTAAACTAGGGATATTTGAAAACCTCAACAAACATGCATTTCCTCTTTCTAACGGACAGGCACTATTTGCATTCAGCTATAAAGAAAAATTTCCAATTAATGGCTGGAAAGTTTATGATCCAGTATCTGAATATAAGAGACAGGGCTTGCCAAATGAGAGTTGGAAAATATCCAAAATAAACAGTAATTATGAGTTCTGTGACACCTACCCTGCCATCATTGTTGTGCCAACTAGTGTAAAAGATGATGACCTTTCAAAAGTGGCAGCTTTTCGAGCAAAAGGCAGAGTCCCTGTGTTGTCATGGATTCATCCGGAAAGTCAAGCAACGATTACCCGTTGCAGCCAGCCACTTGTGGGTCCCAATGATAAGCGCTGCAAAGAGGATGAAAAATACTTGCAAACAATAATGGATGCTAACGCACAGTCACACAAGCTTATCATCTTTGATGCTCGACAAAACAGTGTCGCTGATACCAACAAGACAAAGGGTGGAGGATATGAAAGTGAAAGTGCTTACCCAAATGCAGAACTTGTGTTCTTGGAGATCCACAACATTCATGTCATGCGAGAGTCACTACGCAAATTAAAAGAGATTGTGTACCCTTCGATCGATGAGGCGCGGTGGCTCTCCAATGTGGATGGGACGCATTGGCTGGAATATATAAGGATGCTGCTTGCTGGGGCAGTAAGAATTGCTGATAAAATAGAATCTGGGAAAACATCTGTGGTGGTGCATTGCAGCGACGGTTGGGACCGAACAGCCCAGCTCACATCTCTGGCTATGCTAATGTTGGACAGTTACTACAGGACCATTAAAGGATTTGAAACTCTCGTAGAAAAGGAGTGGATAAGCTTTGGACACAGGTTTGCACTGCGAGTGGGCCATGGTAATGACAACCATGCGGATGCTGACCGATCTCCCATATTTCTGCAGTTTGTTGATTGTGTTTGGCAAATGACAAGGCAGTTTCCTTCAGCATTCGAGTTTAATGAGCTATTCTTGATTACAATTTTGGATCACCTTTATAGCTGTCTTTTTGGGACCTTTTTGTGCAACTGTGAACAGCAGCGATTCAAAGAGGATGTATATACAAAGACGATATCTTTATGGTCGTATATCAATAGCCAGCTAGACGAGTTTTCTAATCCCTTCTTTGTGAATTATGAAAACCACGTGTTATATCCTGTTGCTAGTCTGAGTCATTTGGAATTGTGGGTAAATTATTATGTACGATGGAATCCACGGATGAGACCTCAGATGCCCATTCACCAGAATCTCAAGGAGCTGCTGGCCGTCAGGGCGGAGCTGCAGAAGCGTGTGGAGGGCCTACAGCGGGAGGTGGCCACGCGCGCCGTCTCATCCTCATCTGAGCGGGGCTCCTCGCCCTCCCACTCCGCCACCTCCGTCCACACCTCGGTCTGATGGGCGAGAAATATGTAATCCCCTGGCTGACTAGGACTGTTAAACATAGTGTGGACTGGATGATGCCTTCGACAAACCAGAGAAGCCAAGTTGGGGGGAGCTGGTGCCTGGAGTGGGCCCTGTGCACCTCACCTGGCGGAGGCTGGGGGGGGCTCTGTCAGCAGGACCCTAGAGGAGACTCTCATTCGATTTTAAAGAAGCACAACGGGTCATTTTCCTTTGTATGTTCCTAGCGCAGAACTGTTTCTAAAACAACTTGAAGTATAGTTTTGTTATCTAAGCAATTTTTGTTTTAAGTAAGTAAGTGTACTAGAATGCGAAGCCGTTATGGTTCAGGTTTTTAAAAACTGGTACAGTATTGTATTTGTCTCATCTGTTGCACTGTATTTCAATCATCTGTAATTAAAATGATCATATGTTTGCTCCCTGGTCTTTTTTAAGTAAGTAAGTAAGTATCTTAGTAGATTTTTCCTTTGAGGAAAATCGGTAATAAAATAACATGGATTGAATGTTTACTGTGCGTCAAGCACAGTTAATATATGATGATGTAAAGTAACTAACTTTATGTGATTTAATTCATTCAGTAAATTGT
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 31391, GC content failed 2478, low tm 14347, high tm 8293, high end compl 34, high repeat similarity 35, long poly-x seq 71, ok 6133
PRIMER_RIGHT_EXPLAIN=considered 31406, GC content failed 2365, low tm 14974, high tm 7760, high end compl 1, high repeat similarity 5, long poly-x seq 65, ok 6236
PRIMER_PAIR_EXPLAIN=considered 319, unacceptable product size 262, high any compl 1, high end compl 5, ok 51
PRIMER_PAIR_PENALTY=0.0714
PRIMER_LEFT_PENALTY=0.048411
PRIMER_RIGHT_PENALTY=0.022975
PRIMER_LEFT_SEQUENCE=GATAAGCTTTGGACACAGGTTTG
PRIMER_RIGHT_SEQUENCE=AGCTGGCTATTGATATACGACCA
PRIMER_LEFT=1585,23
PRIMER_RIGHT=1869,23
PRIMER_LEFT_TM=60.048
PRIMER_RIGHT_TM=60.023
PRIMER_LEFT_GC_PERCENT=43.478
PRIMER_RIGHT_GC_PERCENT=43.478
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_MISPRIMING_SCORE=23.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=285
PRIMER_PAIR_PENALTY_1=0.0800
PRIMER_LEFT_1_PENALTY=0.062402
PRIMER_RIGHT_1_PENALTY=0.017590
PRIMER_LEFT_1_SEQUENCE=AATAATGGATGCTAACGCACAGT
PRIMER_RIGHT_1_SEQUENCE=GTCCAAAGCTTATCCACTCCTTT
PRIMER_LEFT_1=1141,23
PRIMER_RIGHT_1=1598,23
PRIMER_LEFT_1_TM=59.938
PRIMER_RIGHT_1_TM=60.018
PRIMER_LEFT_1_GC_PERCENT=39.130
PRIMER_RIGHT_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, MER34 a consensus
PRIMER_PAIR_1_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_1_END_STABILITY=6.1000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=458
PRIMER_PAIR_PENALTY_2=0.0854
PRIMER_LEFT_2_PENALTY=0.062402
PRIMER_RIGHT_2_PENALTY=0.022975
PRIMER_LEFT_2_SEQUENCE=AATAATGGATGCTAACGCACAGT
PRIMER_RIGHT_2_SEQUENCE=AGCTGGCTATTGATATACGACCA
PRIMER_LEFT_2=1141,23
PRIMER_RIGHT_2=1869,23
PRIMER_LEFT_2_TM=59.938
PRIMER_RIGHT_2_TM=60.023
PRIMER_LEFT_2_GC_PERCENT=39.130
PRIMER_RIGHT_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_2_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_2_MISPRIMING_SCORE=24.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_2_END_STABILITY=6.1000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=729
PRIMER_PAIR_PENALTY_3=0.1108
PRIMER_LEFT_3_PENALTY=0.062402
PRIMER_RIGHT_3_PENALTY=0.048411
PRIMER_LEFT_3_SEQUENCE=AATAATGGATGCTAACGCACAGT
PRIMER_RIGHT_3_SEQUENCE=CAAACCTGTGTCCAAAGCTTATC
PRIMER_LEFT_3=1141,23
PRIMER_RIGHT_3=1607,23
PRIMER_LEFT_3_TM=59.938
PRIMER_RIGHT_3_TM=60.048
PRIMER_LEFT_3_GC_PERCENT=39.130
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_3_MISPRIMING_SCORE=11.00, LTR12 LTR from human ERV9 endogenous retroviral sequence (HRES-1/1)
PRIMER_PAIR_3_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_3_END_STABILITY=6.1000
PRIMER_RIGHT_3_END_STABILITY=5.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=467
PRIMER_PAIR_PENALTY_4=0.1203
PRIMER_LEFT_4_PENALTY=0.102704
PRIMER_RIGHT_4_PENALTY=0.017590
PRIMER_LEFT_4_SEQUENCE=AAGCTTATCATCTTTGATGCTCG
PRIMER_RIGHT_4_SEQUENCE=GTCCAAAGCTTATCCACTCCTTT
PRIMER_LEFT_4=1169,23
PRIMER_RIGHT_4=1598,23
PRIMER_LEFT_4_TM=59.897
PRIMER_RIGHT_4_TM=60.018
PRIMER_LEFT_4_GC_PERCENT=39.130
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, reverse MER42a MER42 repetitive sequence: subfamily MER42a - a consensus
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, MER34 a consensus
PRIMER_PAIR_4_MISPRIMING_SCORE=20.00, PAB Human pseudoautosomal boundary-like sequence.
PRIMER_LEFT_4_END_STABILITY=9.9000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=430
PRIMER_PAIR_PENALTY_5=0.1308
PRIMER_LEFT_5_PENALTY=0.048411
PRIMER_RIGHT_5_PENALTY=0.082348
PRIMER_LEFT_5_SEQUENCE=GATAAGCTTTGGACACAGGTTTG
PRIMER_RIGHT_5_SEQUENCE=TTTAGAAACAGTTCTGCGCTAGG
PRIMER_LEFT_5=1585,23
PRIMER_RIGHT_5=2412,23
PRIMER_LEFT_5_TM=60.048
PRIMER_RIGHT_5_TM=60.082
PRIMER_LEFT_5_GC_PERCENT=43.478
PRIMER_RIGHT_5_GC_PERCENT=43.478
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=5.00
PRIMER_LEFT_5_SELF_END=2.00
PRIMER_RIGHT_5_SELF_END=2.00
PRIMER_LEFT_5_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_5_MISPRIMING_SCORE=11.00, MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_PAIR_5_MISPRIMING_SCORE=20.00, MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_LEFT_5_END_STABILITY=7.0000
PRIMER_RIGHT_5_END_STABILITY=7.2000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_5=828
PRIMER_PAIR_PENALTY_6=0.1411
PRIMER_LEFT_6_PENALTY=0.123491
PRIMER_RIGHT_6_PENALTY=0.017590
PRIMER_LEFT_6_SEQUENCE=CATGGAGACAATTCCTGTGGTAT
PRIMER_RIGHT_6_SEQUENCE=GTCCAAAGCTTATCCACTCCTTT
PRIMER_LEFT_6=656,23
PRIMER_RIGHT_6=1598,23
PRIMER_LEFT_6_TM=60.123
PRIMER_RIGHT_6_TM=60.018
PRIMER_LEFT_6_GC_PERCENT=43.478
PRIMER_RIGHT_6_GC_PERCENT=43.478
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=3.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_MISPRIMING_SCORE=12.00, reverse MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_RIGHT_6_MISPRIMING_SCORE=12.00, MER34 a consensus
PRIMER_PAIR_6_MISPRIMING_SCORE=20.00, reverse MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_LEFT_6_END_STABILITY=6.8000
PRIMER_RIGHT_6_END_STABILITY=8.5000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_6=943
PRIMER_PAIR_PENALTY_7=0.1506
PRIMER_LEFT_7_PENALTY=0.048411
PRIMER_RIGHT_7_PENALTY=0.102207
PRIMER_LEFT_7_SEQUENCE=GATAAGCTTTGGACACAGGTTTG
PRIMER_RIGHT_7_SEQUENCE=TTACCCACAATTCCAAATGACTC
PRIMER_LEFT_7=1585,23
PRIMER_RIGHT_7=1958,23
PRIMER_LEFT_7_TM=60.048
PRIMER_RIGHT_7_TM=60.102
PRIMER_LEFT_7_GC_PERCENT=43.478
PRIMER_RIGHT_7_GC_PERCENT=39.130
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=2.00
PRIMER_RIGHT_7_SELF_END=3.00
PRIMER_LEFT_7_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_7_MISPRIMING_SCORE=11.00, THE1b consensus sequence
PRIMER_PAIR_7_MISPRIMING_SCORE=23.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_7_END_STABILITY=7.0000
PRIMER_RIGHT_7_END_STABILITY=6.1000
PRIMER_PAIR_7_COMPL_ANY=6.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_7=374
PRIMER_PAIR_PENALTY_8=0.1511
PRIMER_LEFT_8_PENALTY=0.102704
PRIMER_RIGHT_8_PENALTY=0.048411
PRIMER_LEFT_8_SEQUENCE=AAGCTTATCATCTTTGATGCTCG
PRIMER_RIGHT_8_SEQUENCE=CAAACCTGTGTCCAAAGCTTATC
PRIMER_LEFT_8=1169,23
PRIMER_RIGHT_8=1607,23
PRIMER_LEFT_8_TM=59.897
PRIMER_RIGHT_8_TM=60.048
PRIMER_LEFT_8_GC_PERCENT=39.130
PRIMER_RIGHT_8_GC_PERCENT=43.478
PRIMER_LEFT_8_SELF_ANY=6.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=2.00
PRIMER_RIGHT_8_SELF_END=2.00
PRIMER_LEFT_8_MISPRIMING_SCORE=12.00, reverse MER42a MER42 repetitive sequence: subfamily MER42a - a consensus
PRIMER_RIGHT_8_MISPRIMING_SCORE=11.00, LTR12 LTR from human ERV9 endogenous retroviral sequence (HRES-1/1)
PRIMER_PAIR_8_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_8_END_STABILITY=9.9000
PRIMER_RIGHT_8_END_STABILITY=5.9000
PRIMER_PAIR_8_COMPL_ANY=6.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_8=439
PRIMER_PAIR_PENALTY_9=0.1552
PRIMER_LEFT_9_PENALTY=0.137562
PRIMER_RIGHT_9_PENALTY=0.017590
PRIMER_LEFT_9_SEQUENCE=GTTCTTGGAGATCCACAACATTC
PRIMER_RIGHT_9_SEQUENCE=GTCCAAAGCTTATCCACTCCTTT
PRIMER_LEFT_9=1273,23
PRIMER_RIGHT_9=1598,23
PRIMER_LEFT_9_TM=59.862
PRIMER_RIGHT_9_TM=60.018
PRIMER_LEFT_9_GC_PERCENT=43.478
PRIMER_RIGHT_9_GC_PERCENT=43.478
PRIMER_LEFT_9_SELF_ANY=6.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=2.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_MISPRIMING_SCORE=12.00, MER39 a consensus
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, MER34 a consensus
PRIMER_PAIR_9_MISPRIMING_SCORE=21.00, MER39 a consensus
PRIMER_LEFT_9_END_STABILITY=6.9000
PRIMER_RIGHT_9_END_STABILITY=8.5000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_9=326
PRIMER_PAIR_PENALTY_10=0.1555
PRIMER_LEFT_10_PENALTY=0.102207
PRIMER_RIGHT_10_PENALTY=0.053256
PRIMER_LEFT_10_SEQUENCE=GAGTCATTTGGAATTGTGGGTAA
PRIMER_RIGHT_10_SEQUENCE=TGACGCACAGTAAACATTCAATC
PRIMER_LEFT_10=1936,23
PRIMER_RIGHT_10=2686,23
PRIMER_LEFT_10_TM=60.102
PRIMER_RIGHT_10_TM=60.053
PRIMER_LEFT_10_GC_PERCENT=39.130
PRIMER_RIGHT_10_GC_PERCENT=39.130
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=5.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=3.00
PRIMER_LEFT_10_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_10_MISPRIMING_SCORE=11.00, LTR5 LTR from human endogenous retrovirus 5' LTR, clone HERV-K18
PRIMER_PAIR_10_MISPRIMING_SCORE=21.00, MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_LEFT_10_END_STABILITY=7.2000
PRIMER_RIGHT_10_END_STABILITY=6.9000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_10=751
PRIMER_PAIR_PENALTY_11=0.1567
PRIMER_LEFT_11_PENALTY=0.048411
PRIMER_RIGHT_11_PENALTY=0.108280
PRIMER_LEFT_11_SEQUENCE=GATAAGCTTTGGACACAGGTTTG
PRIMER_RIGHT_11_SEQUENCE=AGTCAGCCAGGGGATTACATATT
PRIMER_LEFT_11=1585,23
PRIMER_RIGHT_11=2193,23
PRIMER_LEFT_11_TM=60.048
PRIMER_RIGHT_11_TM=60.108
PRIMER_LEFT_11_GC_PERCENT=43.478
PRIMER_RIGHT_11_GC_PERCENT=43.478
PRIMER_LEFT_11_SELF_ANY=6.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=2.00
PRIMER_RIGHT_11_SELF_END=2.00
PRIMER_LEFT_11_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, MER11A a consensus
PRIMER_PAIR_11_MISPRIMING_SCORE=22.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_11_END_STABILITY=7.0000
PRIMER_RIGHT_11_END_STABILITY=5.8000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_11=609
PRIMER_PAIR_PENALTY_12=0.1605
PRIMER_LEFT_12_PENALTY=0.137562
PRIMER_RIGHT_12_PENALTY=0.022975
PRIMER_LEFT_12_SEQUENCE=GTTCTTGGAGATCCACAACATTC
PRIMER_RIGHT_12_SEQUENCE=AGCTGGCTATTGATATACGACCA
PRIMER_LEFT_12=1273,23
PRIMER_RIGHT_12=1869,23
PRIMER_LEFT_12_TM=59.862
PRIMER_RIGHT_12_TM=60.023
PRIMER_LEFT_12_GC_PERCENT=43.478
PRIMER_RIGHT_12_GC_PERCENT=43.478
PRIMER_LEFT_12_SELF_ANY=6.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=2.00
PRIMER_RIGHT_12_SELF_END=3.00
PRIMER_LEFT_12_MISPRIMING_SCORE=12.00, MER39 a consensus
PRIMER_RIGHT_12_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_12_MISPRIMING_SCORE=22.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_12_END_STABILITY=6.9000
PRIMER_RIGHT_12_END_STABILITY=7.9000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_12=597
PRIMER_PAIR_PENALTY_13=0.1615
PRIMER_LEFT_13_PENALTY=0.108280
PRIMER_RIGHT_13_PENALTY=0.053256
PRIMER_LEFT_13_SEQUENCE=AATATGTAATCCCCTGGCTGACT
PRIMER_RIGHT_13_SEQUENCE=TGACGCACAGTAAACATTCAATC
PRIMER_LEFT_13=2171,23
PRIMER_RIGHT_13=2686,23
PRIMER_LEFT_13_TM=60.108
PRIMER_RIGHT_13_TM=60.053
PRIMER_LEFT_13_GC_PERCENT=43.478
PRIMER_RIGHT_13_GC_PERCENT=39.130
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=5.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=3.00
PRIMER_LEFT_13_MISPRIMING_SCORE=11.00, MER31 a consensus
PRIMER_RIGHT_13_MISPRIMING_SCORE=11.00, LTR5 LTR from human endogenous retrovirus 5' LTR, clone HERV-K18
PRIMER_PAIR_13_MISPRIMING_SCORE=20.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_13_END_STABILITY=6.4000
PRIMER_RIGHT_13_END_STABILITY=6.9000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_13=516
PRIMER_PAIR_PENALTY_14=0.1646
PRIMER_LEFT_14_PENALTY=0.062402
PRIMER_RIGHT_14_PENALTY=0.102207
PRIMER_LEFT_14_SEQUENCE=AATAATGGATGCTAACGCACAGT
PRIMER_RIGHT_14_SEQUENCE=TTACCCACAATTCCAAATGACTC
PRIMER_LEFT_14=1141,23
PRIMER_RIGHT_14=1958,23
PRIMER_LEFT_14_TM=59.938
PRIMER_RIGHT_14_TM=60.102
PRIMER_LEFT_14_GC_PERCENT=39.130
PRIMER_RIGHT_14_GC_PERCENT=39.130
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=3.00
PRIMER_RIGHT_14_SELF_END=3.00
PRIMER_LEFT_14_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_14_MISPRIMING_SCORE=11.00, THE1b consensus sequence
PRIMER_PAIR_14_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_14_END_STABILITY=6.1000
PRIMER_RIGHT_14_END_STABILITY=6.1000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_14=818
PRIMER_PAIR_PENALTY_15=0.1719
PRIMER_LEFT_15_PENALTY=0.123491
PRIMER_RIGHT_15_PENALTY=0.048411
PRIMER_LEFT_15_SEQUENCE=CATGGAGACAATTCCTGTGGTAT
PRIMER_RIGHT_15_SEQUENCE=CAAACCTGTGTCCAAAGCTTATC
PRIMER_LEFT_15=656,23
PRIMER_RIGHT_15=1607,23
PRIMER_LEFT_15_TM=60.123
PRIMER_RIGHT_15_TM=60.048
PRIMER_LEFT_15_GC_PERCENT=43.478
PRIMER_RIGHT_15_GC_PERCENT=43.478
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=3.00
PRIMER_RIGHT_15_SELF_END=2.00
PRIMER_LEFT_15_MISPRIMING_SCORE=12.00, reverse MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_RIGHT_15_MISPRIMING_SCORE=11.00, LTR12 LTR from human ERV9 endogenous retroviral sequence (HRES-1/1)
PRIMER_PAIR_15_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_15_END_STABILITY=6.8000
PRIMER_RIGHT_15_END_STABILITY=5.9000
PRIMER_PAIR_15_COMPL_ANY=6.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_15=952
PRIMER_PAIR_PENALTY_16=0.1846
PRIMER_LEFT_16_PENALTY=0.102207
PRIMER_RIGHT_16_PENALTY=0.082348
PRIMER_LEFT_16_SEQUENCE=GAGTCATTTGGAATTGTGGGTAA
PRIMER_RIGHT_16_SEQUENCE=TTTAGAAACAGTTCTGCGCTAGG
PRIMER_LEFT_16=1936,23
PRIMER_RIGHT_16=2412,23
PRIMER_LEFT_16_TM=60.102
PRIMER_RIGHT_16_TM=60.082
PRIMER_LEFT_16_GC_PERCENT=39.130
PRIMER_RIGHT_16_GC_PERCENT=43.478
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=5.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=2.00
PRIMER_LEFT_16_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_16_MISPRIMING_SCORE=11.00, MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_PAIR_16_MISPRIMING_SCORE=21.00, MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_LEFT_16_END_STABILITY=7.2000
PRIMER_RIGHT_16_END_STABILITY=7.2000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_16=477
PRIMER_PAIR_PENALTY_17=0.1859
PRIMER_LEFT_17_PENALTY=0.123491
PRIMER_RIGHT_17_PENALTY=0.062402
PRIMER_LEFT_17_SEQUENCE=CATGGAGACAATTCCTGTGGTAT
PRIMER_RIGHT_17_SEQUENCE=ACTGTGCGTTAGCATCCATTATT
PRIMER_LEFT_17=656,23
PRIMER_RIGHT_17=1163,23
PRIMER_LEFT_17_TM=60.123
PRIMER_RIGHT_17_TM=59.938
PRIMER_LEFT_17_GC_PERCENT=43.478
PRIMER_RIGHT_17_GC_PERCENT=39.130
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=3.00
PRIMER_RIGHT_17_SELF_END=2.00
PRIMER_LEFT_17_MISPRIMING_SCORE=12.00, reverse MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_RIGHT_17_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_17_MISPRIMING_SCORE=21.00, MER42a MER42 repetitive sequence: subfamily MER42a - a consensus
PRIMER_LEFT_17_END_STABILITY=6.8000
PRIMER_RIGHT_17_END_STABILITY=6.2000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_17=508
PRIMER_PAIR_PENALTY_18=0.1860
PRIMER_LEFT_18_PENALTY=0.137562
PRIMER_RIGHT_18_PENALTY=0.048411
PRIMER_LEFT_18_SEQUENCE=GTTCTTGGAGATCCACAACATTC
PRIMER_RIGHT_18_SEQUENCE=CAAACCTGTGTCCAAAGCTTATC
PRIMER_LEFT_18=1273,23
PRIMER_RIGHT_18=1607,23
PRIMER_LEFT_18_TM=59.862
PRIMER_RIGHT_18_TM=60.048
PRIMER_LEFT_18_GC_PERCENT=43.478
PRIMER_RIGHT_18_GC_PERCENT=43.478
PRIMER_LEFT_18_SELF_ANY=6.00
PRIMER_RIGHT_18_SELF_ANY=6.00
PRIMER_LEFT_18_SELF_END=2.00
PRIMER_RIGHT_18_SELF_END=2.00
PRIMER_LEFT_18_MISPRIMING_SCORE=12.00, MER39 a consensus
PRIMER_RIGHT_18_MISPRIMING_SCORE=11.00, LTR12 LTR from human ERV9 endogenous retroviral sequence (HRES-1/1)
PRIMER_PAIR_18_MISPRIMING_SCORE=23.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_18_END_STABILITY=6.9000
PRIMER_RIGHT_18_END_STABILITY=5.9000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_18=335
PRIMER_PAIR_PENALTY_19=0.1889
PRIMER_LEFT_19_PENALTY=0.048411
PRIMER_RIGHT_19_PENALTY=0.140508
PRIMER_LEFT_19_SEQUENCE=GATAAGCTTTGGACACAGGTTTG
PRIMER_RIGHT_19_SEQUENCE=ACAGTTCTGCGCTAGGAACATAC
PRIMER_LEFT_19=1585,23
PRIMER_RIGHT_19=2405,23
PRIMER_LEFT_19_TM=60.048
PRIMER_RIGHT_19_TM=59.859
PRIMER_LEFT_19_GC_PERCENT=43.478
PRIMER_RIGHT_19_GC_PERCENT=47.826
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=2.00
PRIMER_RIGHT_19_SELF_END=2.00
PRIMER_LEFT_19_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_19_MISPRIMING_SCORE=11.00, reverse MER9 a consensus
PRIMER_PAIR_19_MISPRIMING_SCORE=22.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_19_END_STABILITY=7.0000
PRIMER_RIGHT_19_END_STABILITY=5.6000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_19=821
=