PRIMER_SEQUENCE_ID=NM_004358
SEQUENCE=GCAGCCAGTCGCGGAGGCGGGGAGGCTGCGCGGTCAGAGGCGCCTGGAGCGAGCGAATCCTGGCCCACCGCCTGCCCAACCGCGTGACCTTGATTGAGTTAATGAACTTCACGCCTCAGCGTCCAGGTCTGTAAAATGGGGTGTCTAACGCAGACCGTACAGCCCAGCTGGGTTTAGCAAACTTCCGGGAGCCAGTTGGAGCCTCTCCCCATCCCTAGCGGTGATCCCAGGTGACGACATGCCGCGGGGGGTCCTGCGGAGGCCACCCTAGGGCGTTGCTGCTGCCTTTGGGAGTGTGGAGCTCCAAACCATGTCGCGAGAGGCGGATTTTGGGAGGCCGGGATCCTCGCGCCAGGGGGATGTGCGAGGGTGTGGGATAAATCTTAATTCCTCCGGCCCACCCAAAGCCTGGAAATCCAGCCTCCGCGCCTCTTGCCCTGCGGGCCCCGCCCTCAGTCCCGCCCTCATCTAACCCGCTACCCCATTGGTGGCGTCCGGCGGCGCGGCTGCTGTTATTTTTCGAATATATAAGGAGGTGGAAGTGGCAGCTGCAACTAGAGGCTTCCCTGGCTGGTGCCTGAGCCCGGCGTCCCTCGCCCCCCGCCCTCCCCGCATCCCTCTCCTCCCTCGCGCCTGGCCCTGTGGCTCTTCCTCCCTCCCTCCTTCCCCCCCCCCCCACCCCTCGCCCGCTGCCTCCCTCGGCCCAGCCAGCTGTGCCGGCGTTTGTTGGCTGCCCTGCGCCCGGCCCTCCAGCCAGCCTTCTGCCGGCCCCGCCGCGATGGAGGTGCCCCAGCCGGAGCCCGCGCCAGGCTCGGCTCTCAGTCCAGCAGGCGTGTGCGGTGGCGCCCAGCGTCCGGGCCACCTCCCGGGCCTCCTGCTGGGATCTCATGGCCTCCTGGGGTCCCCGGTGCGGGCGGCCGCTTCCTCGCCGGTCACCACCCTCACCCAGACCATGCACGACCTCGCCGGGCTCGGCAGCCGCAGCCGCCTGACGCACCTATCCCTGTCTCGACGGGCATCCGAATCCTCCCTGTCGTCTGAATCCTCCGAATCTTCTGATGCAGGTCTCTGCATGGATTCCCCCAGCCCTATGGACCCCCACATGGCGGAGCAGACGTTTGAACAGGCCATCCAGGCAGCCAGCCGGATCATTCGAAACGAGCAGTTTGCCATCAGACGCTTCCAGTCTATGCCGGTGAGGCTGCTGGGCCACAGCCCCGTGCTTCGGAACATCACCAACTCCCAGGCGCCCGACGGCCGGAGGAAGAGCGAGGCGGGCAGTGGAGCTGCCAGCAGCTCTGGGGAAGACAAGGAGAATGATGGATTTGTCTTCAAGATGCCATGGAAGCCCACACATCCCAGCTCCACCCATGCTCTGGCAGAGTGGGCCAGCCGCAGGGAAGCCTTTGCCCAGAGACCCAGCTCGGCCCCCGACCTGATGTGTCTCAGTCCTGACCGGAAGATGGAAGTGGAGGAGCTCAGCCCCCTGGCCCTAGGTCGCTTCTCTCTGACCCCTGCAGAGGGGGATACTGAGGAAGATGATGGATTTGTGGACATCCTAGAGAGTGACTTAAAGGATGATGATGCAGTTCCCCCAGGCATGGAGAGTCTCATTAGTGCCCCACTGGTCAAGACCTTGGAAAAGGAAGAGGAAAAGGACCTCGTCATGTACAGCAAGTGCCAGCGGCTCTTCCGCTCTCCGTCCATGCCCTGCAGCGTGATCCGGCCCATCCTCAAGAGGCTGGAGCGGCCCCAGGACAGGGACACGCCCGTGCAGAATAAGCGGAGGCGGAGCGTGACCCCTCCTGAGGAGCAGCAGGAGGCTGAGGAACCTAAAGCCCGCGTCCTCCGCTCAAAATCACTGTGTCACGATGAGATCGAGAACCTCCTGGACAGTGACCACCGAGAGCTGATTGGAGATTACTCTAAGGCCTTCCTCCTACAGACAGTAGACGGAAAGCACCAAGACCTCAAGTACATCTCACCAGAAACGATGGTGGCCCTATTGACGGGCAAGTTCAGCAACATCGTGGATAAGTTTGTGATTGTAGACTGCAGATACCCCTATGAATATGAAGGCGGGCACATCAAGACTGCGGTGAACTTGCCCCTGGAACGCGACGCCGAGAGCTTCCTACTGAAGAGCCCCATCGCGCCCTGTAGCCTGGACAAGAGAGTCATCCTCATTTTCCACTGTGAATTCTCATCTGAGCGTGGGCCCCGCATGTGCCGTTTCATCAGGGAACGAGACCGTGCTGTCAACGACTACCCCAGCCTCTACTACCCTGAGATGTATATCCTGAAAGGCGGCTACAAGGAGTTCTTCCCTCAGCACCCGAACTTCTGTGAACCCCAGGACTACCGGCCCATGAACCACGAGGCCTTCAAGGATGAGCTAAAGACCTTCCGCCTCAAGACTCGCAGCTGGGCTGGGGAGCGGAGCCGGCGGGAGCTCTGTAGCCGGCTGCAGGACCAGTGAGGGGCCTGCGCCAGTCCTGCTACCTCCCTTGCCTTTCGAGGCCTGAAGCCAGCTGCCCTATGGGCCTGCCGGGCTGAGGGCCTGCTGGAGGCCTCAGGTGCTGTCCATGGGAAAGATGGTGTGGGTGTCCTGCCTGTCTGCCCCAGCCCAGATTCCCCTGTGTCATCCCATCATTTTCCATATCCTGGTGCCCCCCACCCCTGGAAGAGCCCAGTCTGTTGAGTTAGTTAAGTTGGGTTAATACCAGCTTAAAGGCAGTATTTTGTGTCCTCCAGGAGCTTCTTGTTTCCTTGTTAGGGTTAACCCTTCATCTTCCTGTGTCCTGAAACGCTCCTTTGTGTGTGTGTCAGCTGAGGCTGGGGGAGAGCCGTGGTCCCTGAGGATGGGTCAGAGCTAAACTCCTTCCTGGCCTGAGAGTCAGCTCTCTGCCCTGTGTACTTCCCGGGCCAGGGCTGCCCCTAATCTCTGTAGGAACCGTGGTATGTCTGCCATGTTGCCCCTTTCTCTTTTCCCCTTTCCTGTCCCACCATACGAGCACCTCCAGCCTGAACAGAAGCTCTTACTCTTTCCTATTTCAGTGTTACCTGTGTGCTTGGTCTGTTTGACTTTACGCCCATCTCAGGACACTTCCGTAGACTGTTTAGGTTCCCCTGTCAAATATCAGTTACCCACTCGGTCCCAGTTTTGTTGCCCCAGAAAGGGATGTTATTATCCTTGGGGGCTCCCAGGGCAAGGGTTAAGGCCTGAATCATGAGCCTGCTGGAAGCCCAGCCCCTACTGCTGTGAACCCTGGGGCCTGACTGCTCAGAACTTGCTGCTGTCTTGTTGCGGATGGATGGAAGGTTGGATGGATGGGTGGATGGCCGTGGATGGCCGTGGATGCGCAGTGCCTTGCATACCCAAACCAGGTGGGAGCGTTTTGTTGAGCATGACAGCCTGCAGCAGGAATATATGTGTGCCTATTTGTGTGGACAAAAATATTTACACTTAGGGTTTGGAGCTATTCAAGAGGAAATGTCACAGAAGCAGCTAAACCAAGGACTGAGCACCCTCTGGATTCTGAATCTCAAGATGGGGGCAGGGCTGTGCTTGAAGGCCCTGCTGAGTCATCTGTTAGGGCCTTGGTTCAATAAAGCACTGAGCAAGTTGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 42366, GC content failed 2845, low tm 10626, high tm 21211, high any compl 4, high end compl 31, high repeat similarity 19, long poly-x seq 53, ok 7577
PRIMER_RIGHT_EXPLAIN=considered 42242, GC content failed 2836, low tm 10879, high tm 20729, high any compl 1, high end compl 1, high repeat similarity 4, long poly-x seq 70, ok 7722
PRIMER_PAIR_EXPLAIN=considered 467, unacceptable product size 401, high end compl 8, ok 58
PRIMER_PAIR_PENALTY=0.1172
PRIMER_LEFT_PENALTY=0.053000
PRIMER_RIGHT_PENALTY=0.064245
PRIMER_LEFT_SEQUENCE=AGCAACATCGTGGATAAGTTTGT
PRIMER_RIGHT_SEQUENCE=TAACTCAACAGACTGGGCTCTTC
PRIMER_LEFT=2020,23
PRIMER_RIGHT=2704,23
PRIMER_LEFT_TM=59.947
PRIMER_RIGHT_TM=59.936
PRIMER_LEFT_GC_PERCENT=39.130
PRIMER_RIGHT_GC_PERCENT=47.826
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_MISPRIMING_SCORE=11.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=685
PRIMER_PAIR_PENALTY_1=0.1172
PRIMER_LEFT_1_PENALTY=0.053000
PRIMER_RIGHT_1_PENALTY=0.064245
PRIMER_LEFT_1_SEQUENCE=AGCAACATCGTGGATAAGTTTGT
PRIMER_RIGHT_1_SEQUENCE=GACATACCACGGTTCCTACAGAG
PRIMER_LEFT_1=2020,23
PRIMER_RIGHT_1=2964,23
PRIMER_LEFT_1_TM=59.947
PRIMER_RIGHT_1_TM=59.936
PRIMER_LEFT_1_GC_PERCENT=39.130
PRIMER_RIGHT_1_GC_PERCENT=52.174
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_MISPRIMING_SCORE=11.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, MER21B a consensus
PRIMER_PAIR_1_MISPRIMING_SCORE=21.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=945
PRIMER_PAIR_PENALTY_2=0.1172
PRIMER_LEFT_2_PENALTY=0.053000
PRIMER_RIGHT_2_PENALTY=0.064245
PRIMER_LEFT_2_SEQUENCE=AGCAACATCGTGGATAAGTTTGT
PRIMER_RIGHT_2_SEQUENCE=AGACATACCACGGTTCCTACAGA
PRIMER_LEFT_2=2020,23
PRIMER_RIGHT_2=2965,23
PRIMER_LEFT_2_TM=59.947
PRIMER_RIGHT_2_TM=59.936
PRIMER_LEFT_2_GC_PERCENT=39.130
PRIMER_RIGHT_2_GC_PERCENT=47.826
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_MISPRIMING_SCORE=11.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_2_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_2_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=946
PRIMER_PAIR_PENALTY_3=0.1273
PRIMER_LEFT_3_PENALTY=0.074349
PRIMER_RIGHT_3_PENALTY=0.053000
PRIMER_LEFT_3_SEQUENCE=GAAGATGATGGATTTGTGGACAT
PRIMER_RIGHT_3_SEQUENCE=ACAAACTTATCCACGATGTTGCT
PRIMER_LEFT_3=1534,23
PRIMER_RIGHT_3=2042,23
PRIMER_LEFT_3_TM=60.074
PRIMER_RIGHT_3_TM=59.947
PRIMER_LEFT_3_GC_PERCENT=39.130
PRIMER_RIGHT_3_GC_PERCENT=39.130
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_3_MISPRIMING_SCORE=22.00, reverse Tigger2 Autonomous DNA transposon
PRIMER_LEFT_3_END_STABILITY=6.3000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=509
PRIMER_PAIR_PENALTY_4=0.1273
PRIMER_LEFT_4_PENALTY=0.074349
PRIMER_RIGHT_4_PENALTY=0.053000
PRIMER_LEFT_4_SEQUENCE=AAGATGATGGATTTGTGGACATC
PRIMER_RIGHT_4_SEQUENCE=ACAAACTTATCCACGATGTTGCT
PRIMER_LEFT_4=1535,23
PRIMER_RIGHT_4=2042,23
PRIMER_LEFT_4_TM=60.074
PRIMER_RIGHT_4_TM=59.947
PRIMER_LEFT_4_GC_PERCENT=39.130
PRIMER_RIGHT_4_GC_PERCENT=39.130
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_4_MISPRIMING_SCORE=21.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_4_END_STABILITY=6.3000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=508
PRIMER_PAIR_PENALTY_5=0.1346
PRIMER_LEFT_5_PENALTY=0.064245
PRIMER_RIGHT_5_PENALTY=0.070333
PRIMER_LEFT_5_SEQUENCE=TCTGTAGGAACCGTGGTATGTCT
PRIMER_RIGHT_5_SEQUENCE=GATAATAACATCCCTTTCTGGGG
PRIMER_LEFT_5=2943,23
PRIMER_RIGHT_5=3191,23
PRIMER_LEFT_5_TM=59.936
PRIMER_RIGHT_5_TM=59.930
PRIMER_LEFT_5_GC_PERCENT=47.826
PRIMER_RIGHT_5_GC_PERCENT=43.478
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_MISPRIMING_SCORE=11.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_5_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_5_MISPRIMING_SCORE=20.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_5_END_STABILITY=6.4000
PRIMER_RIGHT_5_END_STABILITY=11.2000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_5=249
PRIMER_PAIR_PENALTY_6=0.1346
PRIMER_LEFT_6_PENALTY=0.064245
PRIMER_RIGHT_6_PENALTY=0.070333
PRIMER_LEFT_6_SEQUENCE=TCTCTGTAGGAACCGTGGTATGT
PRIMER_RIGHT_6_SEQUENCE=GATAATAACATCCCTTTCTGGGG
PRIMER_LEFT_6=2941,23
PRIMER_RIGHT_6=3191,23
PRIMER_LEFT_6_TM=59.936
PRIMER_RIGHT_6_TM=59.930
PRIMER_LEFT_6_GC_PERCENT=47.826
PRIMER_RIGHT_6_GC_PERCENT=43.478
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_MISPRIMING_SCORE=10.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_6_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_6_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_6_END_STABILITY=5.6000
PRIMER_RIGHT_6_END_STABILITY=11.2000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_6=251
PRIMER_PAIR_PENALTY_7=0.1346
PRIMER_LEFT_7_PENALTY=0.064245
PRIMER_RIGHT_7_PENALTY=0.070333
PRIMER_LEFT_7_SEQUENCE=CTCTGTAGGAACCGTGGTATGTC
PRIMER_RIGHT_7_SEQUENCE=GATAATAACATCCCTTTCTGGGG
PRIMER_LEFT_7=2942,23
PRIMER_RIGHT_7=3191,23
PRIMER_LEFT_7_TM=59.936
PRIMER_RIGHT_7_TM=59.930
PRIMER_LEFT_7_GC_PERCENT=52.174
PRIMER_RIGHT_7_GC_PERCENT=43.478
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_MISPRIMING_SCORE=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_7_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_7_MISPRIMING_SCORE=21.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_7_END_STABILITY=6.3000
PRIMER_RIGHT_7_END_STABILITY=11.2000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_7=250
PRIMER_PAIR_PENALTY_8=0.1346
PRIMER_LEFT_8_PENALTY=0.064245
PRIMER_RIGHT_8_PENALTY=0.070333
PRIMER_LEFT_8_SEQUENCE=TCTGTAGGAACCGTGGTATGTCT
PRIMER_RIGHT_8_SEQUENCE=GGATAATAACATCCCTTTCTGGG
PRIMER_LEFT_8=2943,23
PRIMER_RIGHT_8=3192,23
PRIMER_LEFT_8_TM=59.936
PRIMER_RIGHT_8_TM=59.930
PRIMER_LEFT_8_GC_PERCENT=47.826
PRIMER_RIGHT_8_GC_PERCENT=43.478
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_MISPRIMING_SCORE=11.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, MER11B a consensus
PRIMER_PAIR_8_MISPRIMING_SCORE=19.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_8_END_STABILITY=6.4000
PRIMER_RIGHT_8_END_STABILITY=9.7000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_8=250
PRIMER_PAIR_PENALTY_9=0.1346
PRIMER_LEFT_9_PENALTY=0.064245
PRIMER_RIGHT_9_PENALTY=0.070333
PRIMER_LEFT_9_SEQUENCE=TCTCTGTAGGAACCGTGGTATGT
PRIMER_RIGHT_9_SEQUENCE=GGATAATAACATCCCTTTCTGGG
PRIMER_LEFT_9=2941,23
PRIMER_RIGHT_9=3192,23
PRIMER_LEFT_9_TM=59.936
PRIMER_RIGHT_9_TM=59.930
PRIMER_LEFT_9_GC_PERCENT=47.826
PRIMER_RIGHT_9_GC_PERCENT=43.478
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_MISPRIMING_SCORE=10.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, MER11B a consensus
PRIMER_PAIR_9_MISPRIMING_SCORE=20.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_9_END_STABILITY=5.6000
PRIMER_RIGHT_9_END_STABILITY=9.7000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_9=252
PRIMER_PAIR_PENALTY_10=0.1346
PRIMER_LEFT_10_PENALTY=0.064245
PRIMER_RIGHT_10_PENALTY=0.070333
PRIMER_LEFT_10_SEQUENCE=CTCTGTAGGAACCGTGGTATGTC
PRIMER_RIGHT_10_SEQUENCE=GGATAATAACATCCCTTTCTGGG
PRIMER_LEFT_10=2942,23
PRIMER_RIGHT_10=3192,23
PRIMER_LEFT_10_TM=59.936
PRIMER_RIGHT_10_TM=59.930
PRIMER_LEFT_10_GC_PERCENT=52.174
PRIMER_RIGHT_10_GC_PERCENT=43.478
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_MISPRIMING_SCORE=10.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_10_MISPRIMING_SCORE=12.00, MER11B a consensus
PRIMER_PAIR_10_MISPRIMING_SCORE=20.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_10_END_STABILITY=6.3000
PRIMER_RIGHT_10_END_STABILITY=9.7000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_10=251
PRIMER_PAIR_PENALTY_11=0.1417
PRIMER_LEFT_11_PENALTY=0.053000
PRIMER_RIGHT_11_PENALTY=0.088737
PRIMER_LEFT_11_SEQUENCE=AGCAACATCGTGGATAAGTTTGT
PRIMER_RIGHT_11_SEQUENCE=GTAGTAGAGGCTGGGGTAGTCGT
PRIMER_LEFT_11=2020,23
PRIMER_RIGHT_11=2283,23
PRIMER_LEFT_11_TM=59.947
PRIMER_RIGHT_11_TM=60.089
PRIMER_LEFT_11_GC_PERCENT=39.130
PRIMER_RIGHT_11_GC_PERCENT=56.522
PRIMER_LEFT_11_SELF_ANY=3.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=2.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_MISPRIMING_SCORE=11.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_11_MISPRIMING_SCORE=22.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_11_END_STABILITY=7.0000
PRIMER_RIGHT_11_END_STABILITY=7.8000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_11=264
PRIMER_PAIR_PENALTY_12=0.1522
PRIMER_LEFT_12_PENALTY=0.087907
PRIMER_RIGHT_12_PENALTY=0.064245
PRIMER_LEFT_12_SEQUENCE=CAACATCGTGGATAAGTTTGTGA
PRIMER_RIGHT_12_SEQUENCE=GACATACCACGGTTCCTACAGAG
PRIMER_LEFT_12=2022,23
PRIMER_RIGHT_12=2964,23
PRIMER_LEFT_12_TM=59.912
PRIMER_RIGHT_12_TM=59.936
PRIMER_LEFT_12_GC_PERCENT=39.130
PRIMER_RIGHT_12_GC_PERCENT=52.174
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_MISPRIMING_SCORE=12.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_12_MISPRIMING_SCORE=12.00, MER21B a consensus
PRIMER_PAIR_12_MISPRIMING_SCORE=22.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_12_END_STABILITY=6.7000
PRIMER_RIGHT_12_END_STABILITY=6.7000
PRIMER_PAIR_12_COMPL_ANY=6.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_12=943
PRIMER_PAIR_PENALTY_13=0.1522
PRIMER_LEFT_13_PENALTY=0.087907
PRIMER_RIGHT_13_PENALTY=0.064245
PRIMER_LEFT_13_SEQUENCE=CAACATCGTGGATAAGTTTGTGA
PRIMER_RIGHT_13_SEQUENCE=AGACATACCACGGTTCCTACAGA
PRIMER_LEFT_13=2022,23
PRIMER_RIGHT_13=2965,23
PRIMER_LEFT_13_TM=59.912
PRIMER_RIGHT_13_TM=59.936
PRIMER_LEFT_13_GC_PERCENT=39.130
PRIMER_RIGHT_13_GC_PERCENT=47.826
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_MISPRIMING_SCORE=12.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_13_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_13_MISPRIMING_SCORE=23.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_13_END_STABILITY=6.7000
PRIMER_RIGHT_13_END_STABILITY=6.4000
PRIMER_PAIR_13_COMPL_ANY=6.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_13=944
PRIMER_PAIR_PENALTY_14=0.1522
PRIMER_LEFT_14_PENALTY=0.087907
PRIMER_RIGHT_14_PENALTY=0.064245
PRIMER_LEFT_14_SEQUENCE=CAACATCGTGGATAAGTTTGTGA
PRIMER_RIGHT_14_SEQUENCE=TAACTCAACAGACTGGGCTCTTC
PRIMER_LEFT_14=2022,23
PRIMER_RIGHT_14=2704,23
PRIMER_LEFT_14_TM=59.912
PRIMER_RIGHT_14_TM=59.936
PRIMER_LEFT_14_GC_PERCENT=39.130
PRIMER_RIGHT_14_GC_PERCENT=47.826
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=5.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=2.00
PRIMER_LEFT_14_MISPRIMING_SCORE=12.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_14_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_14_MISPRIMING_SCORE=22.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_14_END_STABILITY=6.7000
PRIMER_RIGHT_14_END_STABILITY=6.7000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_14=683
PRIMER_PAIR_PENALTY_15=0.1530
PRIMER_LEFT_15_PENALTY=0.088737
PRIMER_RIGHT_15_PENALTY=0.064245
PRIMER_LEFT_15_SEQUENCE=ACGACTACCCCAGCCTCTACTAC
PRIMER_RIGHT_15_SEQUENCE=AGACATACCACGGTTCCTACAGA
PRIMER_LEFT_15=2261,23
PRIMER_RIGHT_15=2965,23
PRIMER_LEFT_15_TM=60.089
PRIMER_RIGHT_15_TM=59.936
PRIMER_LEFT_15_GC_PERCENT=56.522
PRIMER_RIGHT_15_GC_PERCENT=47.826
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_MISPRIMING_SCORE=12.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_15_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_15_MISPRIMING_SCORE=23.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_15_END_STABILITY=5.1000
PRIMER_RIGHT_15_END_STABILITY=6.4000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_15=705
PRIMER_PAIR_PENALTY_16=0.1530
PRIMER_LEFT_16_PENALTY=0.088737
PRIMER_RIGHT_16_PENALTY=0.064245
PRIMER_LEFT_16_SEQUENCE=ACGACTACCCCAGCCTCTACTAC
PRIMER_RIGHT_16_SEQUENCE=GACATACCACGGTTCCTACAGAG
PRIMER_LEFT_16=2261,23
PRIMER_RIGHT_16=2964,23
PRIMER_LEFT_16_TM=60.089
PRIMER_RIGHT_16_TM=59.936
PRIMER_LEFT_16_GC_PERCENT=56.522
PRIMER_RIGHT_16_GC_PERCENT=52.174
PRIMER_LEFT_16_SELF_ANY=3.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_MISPRIMING_SCORE=12.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_16_MISPRIMING_SCORE=12.00, MER21B a consensus
PRIMER_PAIR_16_MISPRIMING_SCORE=22.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_16_END_STABILITY=5.1000
PRIMER_RIGHT_16_END_STABILITY=6.7000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_16=704
PRIMER_PAIR_PENALTY_17=0.1530
PRIMER_LEFT_17_PENALTY=0.088737
PRIMER_RIGHT_17_PENALTY=0.064245
PRIMER_LEFT_17_SEQUENCE=ACGACTACCCCAGCCTCTACTAC
PRIMER_RIGHT_17_SEQUENCE=TAACTCAACAGACTGGGCTCTTC
PRIMER_LEFT_17=2261,23
PRIMER_RIGHT_17=2704,23
PRIMER_LEFT_17_TM=60.089
PRIMER_RIGHT_17_TM=59.936
PRIMER_LEFT_17_GC_PERCENT=56.522
PRIMER_RIGHT_17_GC_PERCENT=47.826
PRIMER_LEFT_17_SELF_ANY=3.00
PRIMER_RIGHT_17_SELF_ANY=5.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=2.00
PRIMER_LEFT_17_MISPRIMING_SCORE=12.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_17_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_17_MISPRIMING_SCORE=22.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_17_END_STABILITY=5.1000
PRIMER_RIGHT_17_END_STABILITY=6.7000
PRIMER_PAIR_17_COMPL_ANY=6.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_17=444
PRIMER_PAIR_PENALTY_18=0.1591
PRIMER_LEFT_18_PENALTY=0.088737
PRIMER_RIGHT_18_PENALTY=0.070333
PRIMER_LEFT_18_SEQUENCE=ACGACTACCCCAGCCTCTACTAC
PRIMER_RIGHT_18_SEQUENCE=GATAATAACATCCCTTTCTGGGG
PRIMER_LEFT_18=2261,23
PRIMER_RIGHT_18=3191,23
PRIMER_LEFT_18_TM=60.089
PRIMER_RIGHT_18_TM=59.930
PRIMER_LEFT_18_GC_PERCENT=56.522
PRIMER_RIGHT_18_GC_PERCENT=43.478
PRIMER_LEFT_18_SELF_ANY=3.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_MISPRIMING_SCORE=12.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_18_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_18_MISPRIMING_SCORE=23.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_18_END_STABILITY=5.1000
PRIMER_RIGHT_18_END_STABILITY=11.2000
PRIMER_PAIR_18_COMPL_ANY=6.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_18=931
PRIMER_PAIR_PENALTY_19=0.1591
PRIMER_LEFT_19_PENALTY=0.088737
PRIMER_RIGHT_19_PENALTY=0.070333
PRIMER_LEFT_19_SEQUENCE=ACGACTACCCCAGCCTCTACTAC
PRIMER_RIGHT_19_SEQUENCE=GGATAATAACATCCCTTTCTGGG
PRIMER_LEFT_19=2261,23
PRIMER_RIGHT_19=3192,23
PRIMER_LEFT_19_TM=60.089
PRIMER_RIGHT_19_TM=59.930
PRIMER_LEFT_19_GC_PERCENT=56.522
PRIMER_RIGHT_19_GC_PERCENT=43.478
PRIMER_LEFT_19_SELF_ANY=3.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_MISPRIMING_SCORE=12.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_19_MISPRIMING_SCORE=12.00, MER11B a consensus
PRIMER_PAIR_19_MISPRIMING_SCORE=22.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_19_END_STABILITY=5.1000
PRIMER_RIGHT_19_END_STABILITY=9.7000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_19=932
=