PRIMER_SEQUENCE_ID=NM_004418
SEQUENCE=GGAGTCGACCGCTCGGGCAGCGCACCGCCACGAGAGCCCGGGACGCGGGAAAGACCGAAAGGAAGAGGAAGAGGCACCGGTGGCCATGGGGCTGGAGGCGGCGCGCGAGCTGGAGTGCGCGGCGCTGGGCACGCTGCTGCGGGATCCGCGGGAGGCGGAACGCACGCTGCTGCTGGACTGCCGCCCCTTCCTGGCCTTCTGCCGGCGCCACGTGCGCGCCGCGCGGCCAGTGCCTTGGAACGCGCTGCTGCGGCGCCGCGCGCGCGGCCCTCCTGCCGCCGTTCTCGCCTGCCTGCTGCCCGACCGCGCGCTGCGGACGCGCCTGGTCCGCGGGGAGCTGGCGCGGGCCGTGGTGCTGGACGAGGGCAGTGCCTCGGTGGCGGAGCTCCGGCCCGACAGCCCGGCTCATGTGCTGCTGGCCGCGCTGCTGCACGAGACCCGCGCGGGGCCCACTGCCGTGTACTTCCTGCGAGGAGGCTTCGACGGCTTCCAGGGCTGCTGTCCCGATCTGTGCTCTGAGGCCCCCGCCCCTGCGCTGCCGCCAACAGGGGACAAAACCAGCCGCTCCGACTCCAGGGCTCCTGTCTACGACCAGGGTGGCCCTGTGGAGATCTTGCCCTACCTGTTCCTGGGCAGCTGCAGTCACTCGTCAGACCTGCAGGGGCTGCAGGCCTGTGGCATCACAGCCGTCCTCAACGTGTCCGCCAGCTGCCCCAACCACTTTGAGGGCCTTTTCCGCTACAAGAGTATCCCTGTGGAGGACAACCAGATGGTGGAGATCAGTGCCTGGTTCCAGGAGGCCATAGGCTTCATTGACTGGGTGAAGAACAGCGGAGGCCGGGTGCTGGTGCACTGCCAGGCGGGTATCTCGCGCTCTGCCACCATCTGTCTGGCATACCTCATGCAGAGTCGCCGTGTGCGGCTGGACGAGGCCTTTGACTTCGTTAAGCAGCGCCGGGGGGTCATCTCCCCCAACTTCAGTTTCATGGGGCAGCTGCTGCAGTTTGAGACCCAGGTGCTGTGTCACTGAGGTGGTGCCCCTCTGCCTGCCTGCCCCACTGTGCTGGCAGGAGCTGACTGTGGACTGGTGGGCTCCCCTCTGGGCCAGCACAGTCCCCTCACCTCCGGCAGGGCTGCTACCTCCTCAGAGTTTCAGAAGCCCCCACATGGGGGCTCTAGGAATGCCGGCATGCTGGTCTTTCCGACCTGGTGCTCTTCTGCTGGGGGACTGAGGCTGGCCCTCATTCGGGGTCGGGAACCAAGGGTGTGTCTGCTCTTTCCCTCCCCATCCTCTGGCAGAAATCAGCTAGACGCTATACCGTGGACTCTCCCTGGTCCACCACCATGTTGAAGCCCTTGGCAGCCTGAGAGCTCCAAGGAACAAGCTGTGACAACCAGGAGCCCTGTCTGTGGGTTCGTCTGCCCAGGGCCTGGAGCCCAAGCCCTGTGTTCCTGGGGAAGCTGGGGACTTGGGAAGTGATGGGTGTGTCATGTTGCGTGTGTCTGTCTGTGAGCCTTTCACACCTGTGCTGGCGCTGGAAAATTATTTGTGCTCAGCTGACATTTAACACTTCCTCCCCCGCTTCCTCCTAGCCCTGTGGGCAGGGGTTGGAAACTTAGCACTTTATATTTATACAGAACATTCAGGATTTGTCAATAAAATATTGTTATATTTAAAAAAAAAAAAAAAAAAAAAAAAAAA
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 19078, GC content failed 2010, low tm 2904, high tm 11247, high end compl 34, high repeat similarity 51, long poly-x seq 21, ok 2811
PRIMER_RIGHT_EXPLAIN=considered 19080, GC content failed 2385, low tm 3227, high tm 10601, high end compl 10, high repeat similarity 9, long poly-x seq 21, ok 2827
PRIMER_PAIR_EXPLAIN=considered 739, unacceptable product size 677, high end compl 2, ok 60
PRIMER_PAIR_PENALTY=0.1460
PRIMER_LEFT_PENALTY=0.004943
PRIMER_RIGHT_PENALTY=0.141011
PRIMER_LEFT_SEQUENCE=TACAAGAGTATCCCTGTGGAGGA
PRIMER_RIGHT_SEQUENCE=CTGAGCACAAATAATTTTCCAGC
PRIMER_LEFT=739,23
PRIMER_RIGHT=1556,23
PRIMER_LEFT_TM=60.005
PRIMER_RIGHT_TM=60.141
PRIMER_LEFT_GC_PERCENT=47.826
PRIMER_RIGHT_GC_PERCENT=39.130
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_MISPRIMING_SCORE=11.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=818
PRIMER_PAIR_PENALTY_1=0.1460
PRIMER_LEFT_1_PENALTY=0.004943
PRIMER_RIGHT_1_PENALTY=0.141011
PRIMER_LEFT_1_SEQUENCE=TACAAGAGTATCCCTGTGGAGGA
PRIMER_RIGHT_1_SEQUENCE=AGCTGAGCACAAATAATTTTCCA
PRIMER_LEFT_1=739,23
PRIMER_RIGHT_1=1558,23
PRIMER_LEFT_1_TM=60.005
PRIMER_RIGHT_1_TM=60.141
PRIMER_LEFT_1_GC_PERCENT=47.826
PRIMER_RIGHT_1_GC_PERCENT=34.783
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, MER7B Nonautonomous DNA transposon
PRIMER_PAIR_1_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=820
PRIMER_PAIR_PENALTY_2=0.1583
PRIMER_LEFT_2_PENALTY=0.004943
PRIMER_RIGHT_2_PENALTY=0.153359
PRIMER_LEFT_2_SEQUENCE=TACAAGAGTATCCCTGTGGAGGA
PRIMER_RIGHT_2_SEQUENCE=CACGGTATAGCGTCTAGCTGATT
PRIMER_LEFT_2=739,23
PRIMER_RIGHT_2=1322,23
PRIMER_LEFT_2_TM=60.005
PRIMER_RIGHT_2_TM=59.847
PRIMER_LEFT_2_GC_PERCENT=47.826
PRIMER_RIGHT_2_GC_PERCENT=47.826
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_2_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_2_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=584
PRIMER_PAIR_PENALTY_3=0.5352
PRIMER_LEFT_3_PENALTY=0.004943
PRIMER_RIGHT_3_PENALTY=0.530233
PRIMER_LEFT_3_SEQUENCE=TACAAGAGTATCCCTGTGGAGGA
PRIMER_RIGHT_3_SEQUENCE=CGGTATAGCGTCTAGCTGATTTC
PRIMER_LEFT_3=739,23
PRIMER_RIGHT_3=1320,23
PRIMER_LEFT_3_TM=60.005
PRIMER_RIGHT_3_TM=59.470
PRIMER_LEFT_3_GC_PERCENT=47.826
PRIMER_RIGHT_3_GC_PERCENT=47.826
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_3_MISPRIMING_SCORE=24.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=582
PRIMER_PAIR_PENALTY_4=0.6186
PRIMER_LEFT_4_PENALTY=0.477595
PRIMER_RIGHT_4_PENALTY=0.141011
PRIMER_LEFT_4_SEQUENCE=CTTTTCCGCTACAAGAGTATCCC
PRIMER_RIGHT_4_SEQUENCE=AGCTGAGCACAAATAATTTTCCA
PRIMER_LEFT_4=730,23
PRIMER_RIGHT_4=1558,23
PRIMER_LEFT_4_TM=60.478
PRIMER_RIGHT_4_TM=60.141
PRIMER_LEFT_4_GC_PERCENT=47.826
PRIMER_RIGHT_4_GC_PERCENT=34.783
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, MER7B Nonautonomous DNA transposon
PRIMER_PAIR_4_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_4_END_STABILITY=9.3000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=829
PRIMER_PAIR_PENALTY_5=0.6186
PRIMER_LEFT_5_PENALTY=0.477595
PRIMER_RIGHT_5_PENALTY=0.141011
PRIMER_LEFT_5_SEQUENCE=CTTTTCCGCTACAAGAGTATCCC
PRIMER_RIGHT_5_SEQUENCE=CTGAGCACAAATAATTTTCCAGC
PRIMER_LEFT_5=730,23
PRIMER_RIGHT_5=1556,23
PRIMER_LEFT_5_TM=60.478
PRIMER_RIGHT_5_TM=60.141
PRIMER_LEFT_5_GC_PERCENT=47.826
PRIMER_RIGHT_5_GC_PERCENT=39.130
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=7.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=3.00
PRIMER_LEFT_5_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_5_MISPRIMING_SCORE=11.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_5_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_5_END_STABILITY=9.3000
PRIMER_RIGHT_5_END_STABILITY=9.7000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_5=827
PRIMER_PAIR_PENALTY_6=0.6186
PRIMER_LEFT_6_PENALTY=0.477595
PRIMER_RIGHT_6_PENALTY=0.141011
PRIMER_LEFT_6_SEQUENCE=CCTTTTCCGCTACAAGAGTATCC
PRIMER_RIGHT_6_SEQUENCE=AGCTGAGCACAAATAATTTTCCA
PRIMER_LEFT_6=729,23
PRIMER_RIGHT_6=1558,23
PRIMER_LEFT_6_TM=60.478
PRIMER_RIGHT_6_TM=60.141
PRIMER_LEFT_6_GC_PERCENT=47.826
PRIMER_RIGHT_6_GC_PERCENT=34.783
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=7.00
PRIMER_LEFT_6_SELF_END=2.00
PRIMER_RIGHT_6_SELF_END=3.00
PRIMER_LEFT_6_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_6_MISPRIMING_SCORE=12.00, MER7B Nonautonomous DNA transposon
PRIMER_PAIR_6_MISPRIMING_SCORE=23.00, reverse MER42a MER42 repetitive sequence: subfamily MER42a - a consensus
PRIMER_LEFT_6_END_STABILITY=7.1000
PRIMER_RIGHT_6_END_STABILITY=8.5000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_6=830
PRIMER_PAIR_PENALTY_7=0.6186
PRIMER_LEFT_7_PENALTY=0.477595
PRIMER_RIGHT_7_PENALTY=0.141011
PRIMER_LEFT_7_SEQUENCE=CCTTTTCCGCTACAAGAGTATCC
PRIMER_RIGHT_7_SEQUENCE=CTGAGCACAAATAATTTTCCAGC
PRIMER_LEFT_7=729,23
PRIMER_RIGHT_7=1556,23
PRIMER_LEFT_7_TM=60.478
PRIMER_RIGHT_7_TM=60.141
PRIMER_LEFT_7_GC_PERCENT=47.826
PRIMER_RIGHT_7_GC_PERCENT=39.130
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=7.00
PRIMER_LEFT_7_SELF_END=2.00
PRIMER_RIGHT_7_SELF_END=3.00
PRIMER_LEFT_7_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_7_MISPRIMING_SCORE=11.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_7_MISPRIMING_SCORE=23.00, reverse MER42a MER42 repetitive sequence: subfamily MER42a - a consensus
PRIMER_LEFT_7_END_STABILITY=7.1000
PRIMER_RIGHT_7_END_STABILITY=9.7000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_7=828
PRIMER_PAIR_PENALTY_8=0.6310
PRIMER_LEFT_8_PENALTY=0.477595
PRIMER_RIGHT_8_PENALTY=0.153359
PRIMER_LEFT_8_SEQUENCE=CCTTTTCCGCTACAAGAGTATCC
PRIMER_RIGHT_8_SEQUENCE=CACGGTATAGCGTCTAGCTGATT
PRIMER_LEFT_8=729,23
PRIMER_RIGHT_8=1322,23
PRIMER_LEFT_8_TM=60.478
PRIMER_RIGHT_8_TM=59.847
PRIMER_LEFT_8_GC_PERCENT=47.826
PRIMER_RIGHT_8_GC_PERCENT=47.826
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=8.00
PRIMER_LEFT_8_SELF_END=2.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_8_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_8_END_STABILITY=7.1000
PRIMER_RIGHT_8_END_STABILITY=6.9000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_8=594
PRIMER_PAIR_PENALTY_9=0.7460
PRIMER_LEFT_9_PENALTY=0.604956
PRIMER_RIGHT_9_PENALTY=0.141011
PRIMER_LEFT_9_SEQUENCE=GCCTTTTCCGCTACAAGAGTATC
PRIMER_RIGHT_9_SEQUENCE=AGCTGAGCACAAATAATTTTCCA
PRIMER_LEFT_9=728,23
PRIMER_RIGHT_9=1558,23
PRIMER_LEFT_9_TM=60.605
PRIMER_RIGHT_9_TM=60.141
PRIMER_LEFT_9_GC_PERCENT=47.826
PRIMER_RIGHT_9_GC_PERCENT=34.783
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=7.00
PRIMER_LEFT_9_SELF_END=2.00
PRIMER_RIGHT_9_SELF_END=3.00
PRIMER_LEFT_9_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, MER7B Nonautonomous DNA transposon
PRIMER_PAIR_9_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_9_END_STABILITY=5.3000
PRIMER_RIGHT_9_END_STABILITY=8.5000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_9=831
PRIMER_PAIR_PENALTY_10=0.7583
PRIMER_LEFT_10_PENALTY=0.604956
PRIMER_RIGHT_10_PENALTY=0.153359
PRIMER_LEFT_10_SEQUENCE=GCCTTTTCCGCTACAAGAGTATC
PRIMER_RIGHT_10_SEQUENCE=CACGGTATAGCGTCTAGCTGATT
PRIMER_LEFT_10=728,23
PRIMER_RIGHT_10=1322,23
PRIMER_LEFT_10_TM=60.605
PRIMER_RIGHT_10_TM=59.847
PRIMER_LEFT_10_GC_PERCENT=47.826
PRIMER_RIGHT_10_GC_PERCENT=47.826
PRIMER_LEFT_10_SELF_ANY=3.00
PRIMER_RIGHT_10_SELF_ANY=8.00
PRIMER_LEFT_10_SELF_END=2.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_10_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_10_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_10_END_STABILITY=5.3000
PRIMER_RIGHT_10_END_STABILITY=6.9000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_10=595
PRIMER_PAIR_PENALTY_11=0.9190
PRIMER_LEFT_11_PENALTY=0.777944
PRIMER_RIGHT_11_PENALTY=0.141011
PRIMER_LEFT_11_SEQUENCE=AGAGTATCCCTGTGGAGGACAAC
PRIMER_RIGHT_11_SEQUENCE=CTGAGCACAAATAATTTTCCAGC
PRIMER_LEFT_11=743,23
PRIMER_RIGHT_11=1556,23
PRIMER_LEFT_11_TM=60.778
PRIMER_RIGHT_11_TM=60.141
PRIMER_LEFT_11_GC_PERCENT=52.174
PRIMER_RIGHT_11_GC_PERCENT=39.130
PRIMER_LEFT_11_SELF_ANY=3.00
PRIMER_RIGHT_11_SELF_ANY=7.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=3.00
PRIMER_LEFT_11_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_11_MISPRIMING_SCORE=11.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_11_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_11_END_STABILITY=6.4000
PRIMER_RIGHT_11_END_STABILITY=9.7000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_11=814
PRIMER_PAIR_PENALTY_12=0.9190
PRIMER_LEFT_12_PENALTY=0.777944
PRIMER_RIGHT_12_PENALTY=0.141011
PRIMER_LEFT_12_SEQUENCE=AGAGTATCCCTGTGGAGGACAAC
PRIMER_RIGHT_12_SEQUENCE=AGCTGAGCACAAATAATTTTCCA
PRIMER_LEFT_12=743,23
PRIMER_RIGHT_12=1558,23
PRIMER_LEFT_12_TM=60.778
PRIMER_RIGHT_12_TM=60.141
PRIMER_LEFT_12_GC_PERCENT=52.174
PRIMER_RIGHT_12_GC_PERCENT=34.783
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=7.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=3.00
PRIMER_LEFT_12_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_12_MISPRIMING_SCORE=12.00, MER7B Nonautonomous DNA transposon
PRIMER_PAIR_12_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_12_END_STABILITY=6.4000
PRIMER_RIGHT_12_END_STABILITY=8.5000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_12=816
PRIMER_PAIR_PENALTY_13=0.9313
PRIMER_LEFT_13_PENALTY=0.777944
PRIMER_RIGHT_13_PENALTY=0.153359
PRIMER_LEFT_13_SEQUENCE=AGAGTATCCCTGTGGAGGACAAC
PRIMER_RIGHT_13_SEQUENCE=CACGGTATAGCGTCTAGCTGATT
PRIMER_LEFT_13=743,23
PRIMER_RIGHT_13=1322,23
PRIMER_LEFT_13_TM=60.778
PRIMER_RIGHT_13_TM=59.847
PRIMER_LEFT_13_GC_PERCENT=52.174
PRIMER_RIGHT_13_GC_PERCENT=47.826
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=8.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_13_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_13_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_13_END_STABILITY=6.4000
PRIMER_RIGHT_13_END_STABILITY=6.9000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_13=580
PRIMER_PAIR_PENALTY_14=0.9649
PRIMER_LEFT_14_PENALTY=0.823905
PRIMER_RIGHT_14_PENALTY=0.141011
PRIMER_LEFT_14_SEQUENCE=CTACAAGAGTATCCCTGTGGAGG
PRIMER_RIGHT_14_SEQUENCE=CTGAGCACAAATAATTTTCCAGC
PRIMER_LEFT_14=738,23
PRIMER_RIGHT_14=1556,23
PRIMER_LEFT_14_TM=59.176
PRIMER_RIGHT_14_TM=60.141
PRIMER_LEFT_14_GC_PERCENT=52.174
PRIMER_RIGHT_14_GC_PERCENT=39.130
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=7.00
PRIMER_LEFT_14_SELF_END=2.00
PRIMER_RIGHT_14_SELF_END=3.00
PRIMER_LEFT_14_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_14_MISPRIMING_SCORE=11.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_14_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_14_END_STABILITY=9.4000
PRIMER_RIGHT_14_END_STABILITY=9.7000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_14=819
PRIMER_PAIR_PENALTY_15=0.9649
PRIMER_LEFT_15_PENALTY=0.823905
PRIMER_RIGHT_15_PENALTY=0.141011
PRIMER_LEFT_15_SEQUENCE=CTACAAGAGTATCCCTGTGGAGG
PRIMER_RIGHT_15_SEQUENCE=AGCTGAGCACAAATAATTTTCCA
PRIMER_LEFT_15=738,23
PRIMER_RIGHT_15=1558,23
PRIMER_LEFT_15_TM=59.176
PRIMER_RIGHT_15_TM=60.141
PRIMER_LEFT_15_GC_PERCENT=52.174
PRIMER_RIGHT_15_GC_PERCENT=34.783
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=7.00
PRIMER_LEFT_15_SELF_END=2.00
PRIMER_RIGHT_15_SELF_END=3.00
PRIMER_LEFT_15_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_15_MISPRIMING_SCORE=12.00, MER7B Nonautonomous DNA transposon
PRIMER_PAIR_15_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_15_END_STABILITY=9.4000
PRIMER_RIGHT_15_END_STABILITY=8.5000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_15=821
PRIMER_PAIR_PENALTY_16=0.9773
PRIMER_LEFT_16_PENALTY=0.823905
PRIMER_RIGHT_16_PENALTY=0.153359
PRIMER_LEFT_16_SEQUENCE=CTACAAGAGTATCCCTGTGGAGG
PRIMER_RIGHT_16_SEQUENCE=CACGGTATAGCGTCTAGCTGATT
PRIMER_LEFT_16=738,23
PRIMER_RIGHT_16=1322,23
PRIMER_LEFT_16_TM=59.176
PRIMER_RIGHT_16_TM=59.847
PRIMER_LEFT_16_GC_PERCENT=52.174
PRIMER_RIGHT_16_GC_PERCENT=47.826
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=8.00
PRIMER_LEFT_16_SELF_END=2.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_16_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_16_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_16_END_STABILITY=9.4000
PRIMER_RIGHT_16_END_STABILITY=6.9000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_16=585
PRIMER_PAIR_PENALTY_17=1.0078
PRIMER_LEFT_17_PENALTY=0.477595
PRIMER_RIGHT_17_PENALTY=0.530233
PRIMER_LEFT_17_SEQUENCE=CTTTTCCGCTACAAGAGTATCCC
PRIMER_RIGHT_17_SEQUENCE=CGGTATAGCGTCTAGCTGATTTC
PRIMER_LEFT_17=730,23
PRIMER_RIGHT_17=1320,23
PRIMER_LEFT_17_TM=60.478
PRIMER_RIGHT_17_TM=59.470
PRIMER_LEFT_17_GC_PERCENT=47.826
PRIMER_RIGHT_17_GC_PERCENT=47.826
PRIMER_LEFT_17_SELF_ANY=3.00
PRIMER_RIGHT_17_SELF_ANY=6.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=2.00
PRIMER_LEFT_17_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_17_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_17_MISPRIMING_SCORE=24.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_17_END_STABILITY=9.3000
PRIMER_RIGHT_17_END_STABILITY=6.9000
PRIMER_PAIR_17_COMPL_ANY=6.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_17=591
PRIMER_PAIR_PENALTY_18=1.0078
PRIMER_LEFT_18_PENALTY=0.477595
PRIMER_RIGHT_18_PENALTY=0.530233
PRIMER_LEFT_18_SEQUENCE=CCTTTTCCGCTACAAGAGTATCC
PRIMER_RIGHT_18_SEQUENCE=CGGTATAGCGTCTAGCTGATTTC
PRIMER_LEFT_18=729,23
PRIMER_RIGHT_18=1320,23
PRIMER_LEFT_18_TM=60.478
PRIMER_RIGHT_18_TM=59.470
PRIMER_LEFT_18_GC_PERCENT=47.826
PRIMER_RIGHT_18_GC_PERCENT=47.826
PRIMER_LEFT_18_SELF_ANY=3.00
PRIMER_RIGHT_18_SELF_ANY=6.00
PRIMER_LEFT_18_SELF_END=2.00
PRIMER_RIGHT_18_SELF_END=2.00
PRIMER_LEFT_18_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_18_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_18_MISPRIMING_SCORE=24.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_18_END_STABILITY=7.1000
PRIMER_RIGHT_18_END_STABILITY=6.9000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_18=592
PRIMER_PAIR_PENALTY_19=1.0874
PRIMER_LEFT_19_PENALTY=0.004943
PRIMER_RIGHT_19_PENALTY=1.082475
PRIMER_LEFT_19_SEQUENCE=TACAAGAGTATCCCTGTGGAGGA
PRIMER_RIGHT_19_SEQUENCE=GGGGAGGAAGTGTTAAATGTCAG
PRIMER_LEFT_19=739,23
PRIMER_RIGHT_19=1579,23
PRIMER_LEFT_19_TM=60.005
PRIMER_RIGHT_19_TM=61.082
PRIMER_LEFT_19_GC_PERCENT=47.826
PRIMER_RIGHT_19_GC_PERCENT=47.826
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_19_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_19_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_19_END_STABILITY=7.9000
PRIMER_RIGHT_19_END_STABILITY=6.4000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_19=841
=