PRIMER_SEQUENCE_ID=NM_004419
SEQUENCE=AATCGCGAAACCCGGCGAGCGGCGCGCTGGCTATCGAGCGAGCGGGGCGGAACCGGGAGTTGCGCCGCCGCTCGGGCGCCGGGCTCCGTCGCGGCCGCAGCCCCGCGGGTCGCCCTCCCGTGCCTCGCCCGCGGACACCCTGGCCGTGGACACCCTGGCCGTGGGCACCCGCGGGGCGCGGCGCGGGCGCTGCGCGGCGGCGGCGGCGGCATGAAGGTCACGTCGCTCGACGGGCGCCAGCTGCGCAAGATGCTCCGCAAGGAGGCGGCGGCGCGCTGCGTGGTGCTCGACTGCCGGCCCTATCTGGCCTTCGCTGCCTCGAACGTGCGCGGCTCGCTCAACGTCAACCTCAACTCGGTGGTGCTGCGGCGGGCCCGGGGCGGCGCGGTGTCGGCGCGCTACGTGCTGCCCGACGAGGCGGCGCGCGCGCGGCTCCTGCAGGAGGGCGGCGGCGGCGTCGCGGCCGTGGTGGTGCTGGACCAGGGCAGCCGCCACTGGCAGAAGCTGCGAGAGGAGAGCGCCGCGCGTGTCGTCCTCACCTCGCTACTCGCTTGCCTACCCGCCGGCCCGCGGGTCTACTTCCTCAAAGGGGGATATGAGACTTTCTACTCGGAATATCCTGAGTGTTGCGTGGATGTAAAACCCATTTCACAAGAGAAGATTGAGAGTGAGAGAGCCCTCATCAGCCAGTGTGGAAAACCAGTGGTAAATGTCAGCTACAGGCCAGCTTATGACCAGGGTGGCCCAGTTGAAATCCTTCCCTTCCTCTACCTTGGAAGTGCCTACCATGCATCCAAGTGCGAGTTCCTCGCCAACTTGCACATCACAGCCCTGCTGAATGTCTCCCGACGGACCTCCGAGGCCTGCATGACCCACCTACACTACAAATGGATCCCTGTGGAAGACAGCCACACGGCTGACATTAGCTCCCACTTTCAAGAAGCAATAGACTTCATTGACTGTGTCAGGGAAAAGGGAGGCAAGGTCCTGGTCCACTGTGAGGCTGGGATCTCCCGTTCACCCACCATCTGCATGGCTTACCTTATGAAGACCAAGCAGTTCCGCCTGAAGGAGGCCTTCGATTACATCAAGCAGAGGAGGAGCATGGTCTCGCCCAACTTTGGCTTCATGGGCCAGCTCCTGCAGTACGAATCTGAGATCCTGCCCTCCACGCCCAACCCCCAGCCTCCCTCCTGCCAAGGGGAGGCAGCAGGCTCTTCACTGATAGGCCATTTGCAGACACTGAGCCCTGACATGCAGGGTGCCTACTGCACATTCCCTGCCTCGGTGCTGGCACCGGTGCCTACCCACTCAACAGTCTCAGAGCTCAGCAGAAGCCCTGTGGCAACGGCCACATCCTGCTAAAACTGGGATGGAGGAATCGGCCCAGCCCCAAGAGCAACTGTGATTTTTGTTTTTAAGACTCATGGACATTTCATACCTGTGCAATACTGAAGACCTCATTCTGTCATGCTGCCCCAGTGAGATAGTGAGTGGTCACCAGGCTTGCAAATGAACTTCAGACGGACCTCAGGGTAGGTTCTCGGGACTGAAGGAAGGCCAAGCCATTACGGGAGCACAGCATGTGCTGACTACTGTACTTCCAGACCCCTGCCCTCTTGGGACTGCCCAGTCCTTGCACCTCAGAGTTCGCCTTTTCATTTCAAGCATAAGCCAATAAATACCTGCAGCAACGTGGGAGAAAGAAGTTGCTGGACCAGGAGAAAAGGCAGTTATGAAGCCAATTCATTTTGAAGGAAGCACAATTTCCACCTTATTTTTTGAACTTTGGCAGTTTCAATGTCTGTCTCTGTTGCTTCGGGGCATAAGCTGATCACCGTCTAGTTGGGAAAGTCACCCTACAGGGTTTGTAGGGACATGATCAGCATCCTGATTTGAACCCTGAAATGTTGTGTAGACACCCTCTTGGGTCCAATGAGGTAGTTGGTTGAAGTAGCAAGATGTTGGCTTTTCTGGATTTTTTTTGCCATGGGTTCTTCACTGACCTTGGACTTTGGCATGATTCTTAGTCATACTTGAACTTGTCTCATTCCACCTCTTCTCAGAGCAACTCTTCCTTTGGGAAAAGAGTTCTTCAGATCATAGACCAAAAAAGTCATACCTTCGAGGTGGTAGCAGTAGATTCCAGGAGGAGAAGGGTACTTGCTAGGTATCCTGGGTCAGTGGCGGTGCAAACTGGTTTCCTCAGCTGCCTGTCCTTCTGTGTGCTTATGTCTCTTGTGACAATTGTTTTCCTCCCTGCCCCTGGAGGTTGTCTTCAACTGTGGACTTCTGGGATTTGCAGATTTTGCAACGTGGTACTACTTTTTTTTCTTTTTGTCTGTTAGTTATTTCTCCAGGGGAAAAGGCAATAATTTTCTAAGACCCGTGTGAATGTGAAGAAAAGCAGTATGTTACTGGTTGTTGTTGTTGTTCTTGTTTTTTATATGTAAAATAAAAATAGTGAAAGGAG
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 27817, GC content failed 2739, low tm 8530, high tm 11020, high any compl 1, high end compl 18, high repeat similarity 9, long poly-x seq 87, ok 5413
PRIMER_RIGHT_EXPLAIN=considered 27709, GC content failed 2518, low tm 8972, high tm 10560, high end compl 13, high repeat similarity 8, long poly-x seq 103, ok 5535
PRIMER_PAIR_EXPLAIN=considered 470, unacceptable product size 416, high any compl 4, high end compl 2, ok 48
PRIMER_PAIR_PENALTY=0.0174
PRIMER_LEFT_PENALTY=0.003218
PRIMER_RIGHT_PENALTY=0.014220
PRIMER_LEFT_SEQUENCE=CATTTTGAAGGAAGCACAATTTC
PRIMER_RIGHT_SEQUENCE=ACTAAGAATCATGCCAAAGTCCA
PRIMER_LEFT=1747,23
PRIMER_RIGHT=2030,23
PRIMER_LEFT_TM=59.997
PRIMER_RIGHT_TM=60.014
PRIMER_LEFT_GC_PERCENT=34.783
PRIMER_RIGHT_GC_PERCENT=39.130
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_MISPRIMING_SCORE=11.00, L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence
PRIMER_RIGHT_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_MISPRIMING_SCORE=23.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=284
PRIMER_PAIR_PENALTY_1=0.0174
PRIMER_LEFT_1_PENALTY=0.003218
PRIMER_RIGHT_1_PENALTY=0.014220
PRIMER_LEFT_1_SEQUENCE=TCATTTTGAAGGAAGCACAATTT
PRIMER_RIGHT_1_SEQUENCE=ACTAAGAATCATGCCAAAGTCCA
PRIMER_LEFT_1=1746,23
PRIMER_RIGHT_1=2030,23
PRIMER_LEFT_1_TM=59.997
PRIMER_RIGHT_1_TM=60.014
PRIMER_LEFT_1_GC_PERCENT=30.435
PRIMER_RIGHT_1_GC_PERCENT=39.130
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_MISPRIMING_SCORE=12.00, L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_1_MISPRIMING_SCORE=23.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=285
PRIMER_PAIR_PENALTY_2=0.0702
PRIMER_LEFT_2_PENALTY=0.003218
PRIMER_RIGHT_2_PENALTY=0.067019
PRIMER_LEFT_2_SEQUENCE=CATTTTGAAGGAAGCACAATTTC
PRIMER_RIGHT_2_SEQUENCE=GAAGTCCACAGTTGAAGACAACC
PRIMER_LEFT_2=1747,23
PRIMER_RIGHT_2=2292,23
PRIMER_LEFT_2_TM=59.997
PRIMER_RIGHT_2_TM=60.067
PRIMER_LEFT_2_GC_PERCENT=34.783
PRIMER_RIGHT_2_GC_PERCENT=47.826
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_MISPRIMING_SCORE=11.00, L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence
PRIMER_RIGHT_2_MISPRIMING_SCORE=11.00, MLT2D Interspersed repeat MLT2D - a consensus
PRIMER_PAIR_2_MISPRIMING_SCORE=21.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=546
PRIMER_PAIR_PENALTY_3=0.0702
PRIMER_LEFT_3_PENALTY=0.003218
PRIMER_RIGHT_3_PENALTY=0.067019
PRIMER_LEFT_3_SEQUENCE=TCATTTTGAAGGAAGCACAATTT
PRIMER_RIGHT_3_SEQUENCE=GAAGTCCACAGTTGAAGACAACC
PRIMER_LEFT_3=1746,23
PRIMER_RIGHT_3=2292,23
PRIMER_LEFT_3_TM=59.997
PRIMER_RIGHT_3_TM=60.067
PRIMER_LEFT_3_GC_PERCENT=30.435
PRIMER_RIGHT_3_GC_PERCENT=47.826
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence
PRIMER_RIGHT_3_MISPRIMING_SCORE=11.00, MLT2D Interspersed repeat MLT2D - a consensus
PRIMER_PAIR_3_MISPRIMING_SCORE=21.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=547
PRIMER_PAIR_PENALTY_4=0.0830
PRIMER_LEFT_4_PENALTY=0.003218
PRIMER_RIGHT_4_PENALTY=0.079770
PRIMER_LEFT_4_SEQUENCE=CATTTTGAAGGAAGCACAATTTC
PRIMER_RIGHT_4_SEQUENCE=ACATTCACACGGGTCTTAGAAAA
PRIMER_LEFT_4=1747,23
PRIMER_RIGHT_4=2397,23
PRIMER_LEFT_4_TM=59.997
PRIMER_RIGHT_4_TM=59.920
PRIMER_LEFT_4_GC_PERCENT=34.783
PRIMER_RIGHT_4_GC_PERCENT=39.130
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_MISPRIMING_SCORE=11.00, L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, MER42a MER42 repetitive sequence: subfamily MER42a - a consensus
PRIMER_PAIR_4_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=651
PRIMER_PAIR_PENALTY_5=0.0830
PRIMER_LEFT_5_PENALTY=0.003218
PRIMER_RIGHT_5_PENALTY=0.079770
PRIMER_LEFT_5_SEQUENCE=TCATTTTGAAGGAAGCACAATTT
PRIMER_RIGHT_5_SEQUENCE=ACATTCACACGGGTCTTAGAAAA
PRIMER_LEFT_5=1746,23
PRIMER_RIGHT_5=2397,23
PRIMER_LEFT_5_TM=59.997
PRIMER_RIGHT_5_TM=59.920
PRIMER_LEFT_5_GC_PERCENT=30.435
PRIMER_RIGHT_5_GC_PERCENT=39.130
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=5.00
PRIMER_LEFT_5_SELF_END=3.00
PRIMER_RIGHT_5_SELF_END=2.00
PRIMER_LEFT_5_MISPRIMING_SCORE=12.00, L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence
PRIMER_RIGHT_5_MISPRIMING_SCORE=12.00, MER42a MER42 repetitive sequence: subfamily MER42a - a consensus
PRIMER_PAIR_5_MISPRIMING_SCORE=21.00, MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_LEFT_5_END_STABILITY=7.2000
PRIMER_RIGHT_5_END_STABILITY=7.3000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_5=652
PRIMER_PAIR_PENALTY_6=0.0951
PRIMER_LEFT_6_PENALTY=0.028128
PRIMER_RIGHT_6_PENALTY=0.067019
PRIMER_LEFT_6_SEQUENCE=TACTTGCTAGGTATCCTGGGTCA
PRIMER_RIGHT_6_SEQUENCE=GAAGTCCACAGTTGAAGACAACC
PRIMER_LEFT_6=2160,23
PRIMER_RIGHT_6=2292,23
PRIMER_LEFT_6_TM=60.028
PRIMER_RIGHT_6_TM=60.067
PRIMER_LEFT_6_GC_PERCENT=47.826
PRIMER_RIGHT_6_GC_PERCENT=47.826
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=5.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=2.00
PRIMER_LEFT_6_MISPRIMING_SCORE=11.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_6_MISPRIMING_SCORE=11.00, MLT2D Interspersed repeat MLT2D - a consensus
PRIMER_PAIR_6_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_6_END_STABILITY=7.9000
PRIMER_RIGHT_6_END_STABILITY=8.2000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_6=133
PRIMER_PAIR_PENALTY_7=0.1079
PRIMER_LEFT_7_PENALTY=0.028128
PRIMER_RIGHT_7_PENALTY=0.079770
PRIMER_LEFT_7_SEQUENCE=TACTTGCTAGGTATCCTGGGTCA
PRIMER_RIGHT_7_SEQUENCE=ACATTCACACGGGTCTTAGAAAA
PRIMER_LEFT_7=2160,23
PRIMER_RIGHT_7=2397,23
PRIMER_LEFT_7_TM=60.028
PRIMER_RIGHT_7_TM=59.920
PRIMER_LEFT_7_GC_PERCENT=47.826
PRIMER_RIGHT_7_GC_PERCENT=39.130
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=5.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=2.00
PRIMER_LEFT_7_MISPRIMING_SCORE=11.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_7_MISPRIMING_SCORE=12.00, MER42a MER42 repetitive sequence: subfamily MER42a - a consensus
PRIMER_PAIR_7_MISPRIMING_SCORE=22.00, MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_LEFT_7_END_STABILITY=7.9000
PRIMER_RIGHT_7_END_STABILITY=7.3000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_7=238
PRIMER_PAIR_PENALTY_8=0.1099
PRIMER_LEFT_8_PENALTY=0.030116
PRIMER_RIGHT_8_PENALTY=0.079770
PRIMER_LEFT_8_SEQUENCE=TTTTGAACTTTGGCAGTTTCAAT
PRIMER_RIGHT_8_SEQUENCE=ACATTCACACGGGTCTTAGAAAA
PRIMER_LEFT_8=1779,23
PRIMER_RIGHT_8=2397,23
PRIMER_LEFT_8_TM=60.030
PRIMER_RIGHT_8_TM=59.920
PRIMER_LEFT_8_GC_PERCENT=30.435
PRIMER_RIGHT_8_GC_PERCENT=39.130
PRIMER_LEFT_8_SELF_ANY=5.00
PRIMER_RIGHT_8_SELF_ANY=5.00
PRIMER_LEFT_8_SELF_END=3.00
PRIMER_RIGHT_8_SELF_END=2.00
PRIMER_LEFT_8_MISPRIMING_SCORE=12.00, reverse MER34 a consensus
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, MER42a MER42 repetitive sequence: subfamily MER42a - a consensus
PRIMER_PAIR_8_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_8_END_STABILITY=6.9000
PRIMER_RIGHT_8_END_STABILITY=7.3000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_8=619
PRIMER_PAIR_PENALTY_9=0.1328
PRIMER_LEFT_9_PENALTY=0.003218
PRIMER_RIGHT_9_PENALTY=0.129584
PRIMER_LEFT_9_SEQUENCE=CATTTTGAAGGAAGCACAATTTC
PRIMER_RIGHT_9_SEQUENCE=TCTGAGAAGAGGTGGAATGAGAC
PRIMER_LEFT_9=1747,23
PRIMER_RIGHT_9=2066,23
PRIMER_LEFT_9_TM=59.997
PRIMER_RIGHT_9_TM=59.870
PRIMER_LEFT_9_GC_PERCENT=34.783
PRIMER_RIGHT_9_GC_PERCENT=47.826
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=5.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_MISPRIMING_SCORE=11.00, L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_9_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_9_END_STABILITY=6.9000
PRIMER_RIGHT_9_END_STABILITY=6.1000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_9=320
PRIMER_PAIR_PENALTY_10=0.1328
PRIMER_LEFT_10_PENALTY=0.003218
PRIMER_RIGHT_10_PENALTY=0.129584
PRIMER_LEFT_10_SEQUENCE=TCATTTTGAAGGAAGCACAATTT
PRIMER_RIGHT_10_SEQUENCE=TCTGAGAAGAGGTGGAATGAGAC
PRIMER_LEFT_10=1746,23
PRIMER_RIGHT_10=2066,23
PRIMER_LEFT_10_TM=59.997
PRIMER_RIGHT_10_TM=59.870
PRIMER_LEFT_10_GC_PERCENT=30.435
PRIMER_RIGHT_10_GC_PERCENT=47.826
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=5.00
PRIMER_LEFT_10_SELF_END=3.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_MISPRIMING_SCORE=12.00, L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence
PRIMER_RIGHT_10_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_10_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_10_END_STABILITY=7.2000
PRIMER_RIGHT_10_END_STABILITY=6.1000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_10=321
PRIMER_PAIR_PENALTY_11=0.1468
PRIMER_LEFT_11_PENALTY=0.067019
PRIMER_RIGHT_11_PENALTY=0.079770
PRIMER_LEFT_11_SEQUENCE=GGTTGTCTTCAACTGTGGACTTC
PRIMER_RIGHT_11_SEQUENCE=ACATTCACACGGGTCTTAGAAAA
PRIMER_LEFT_11=2270,23
PRIMER_RIGHT_11=2397,23
PRIMER_LEFT_11_TM=60.067
PRIMER_RIGHT_11_TM=59.920
PRIMER_LEFT_11_GC_PERCENT=47.826
PRIMER_RIGHT_11_GC_PERCENT=39.130
PRIMER_LEFT_11_SELF_ANY=5.00
PRIMER_RIGHT_11_SELF_ANY=5.00
PRIMER_LEFT_11_SELF_END=2.00
PRIMER_RIGHT_11_SELF_END=2.00
PRIMER_LEFT_11_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, MER42a MER42 repetitive sequence: subfamily MER42a - a consensus
PRIMER_PAIR_11_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_11_END_STABILITY=6.4000
PRIMER_RIGHT_11_END_STABILITY=7.3000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_11=128
PRIMER_PAIR_PENALTY_12=0.1556
PRIMER_LEFT_12_PENALTY=0.088604
PRIMER_RIGHT_12_PENALTY=0.067019
PRIMER_LEFT_12_SEQUENCE=ACTTTGGCAGTTTCAATGTCTGT
PRIMER_RIGHT_12_SEQUENCE=GAAGTCCACAGTTGAAGACAACC
PRIMER_LEFT_12=1785,23
PRIMER_RIGHT_12=2292,23
PRIMER_LEFT_12_TM=60.089
PRIMER_RIGHT_12_TM=60.067
PRIMER_LEFT_12_GC_PERCENT=39.130
PRIMER_RIGHT_12_GC_PERCENT=47.826
PRIMER_LEFT_12_SELF_ANY=5.00
PRIMER_RIGHT_12_SELF_ANY=5.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=2.00
PRIMER_LEFT_12_MISPRIMING_SCORE=12.00, CER Human D22Z3 repetitive DNA (centromeric DNA)
PRIMER_RIGHT_12_MISPRIMING_SCORE=11.00, MLT2D Interspersed repeat MLT2D - a consensus
PRIMER_PAIR_12_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_12_END_STABILITY=6.4000
PRIMER_RIGHT_12_END_STABILITY=8.2000
PRIMER_PAIR_12_COMPL_ANY=6.00
PRIMER_PAIR_12_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_12=508
PRIMER_PAIR_PENALTY_13=0.1684
PRIMER_LEFT_13_PENALTY=0.088604
PRIMER_RIGHT_13_PENALTY=0.079770
PRIMER_LEFT_13_SEQUENCE=ACTTTGGCAGTTTCAATGTCTGT
PRIMER_RIGHT_13_SEQUENCE=ACATTCACACGGGTCTTAGAAAA
PRIMER_LEFT_13=1785,23
PRIMER_RIGHT_13=2397,23
PRIMER_LEFT_13_TM=60.089
PRIMER_RIGHT_13_TM=59.920
PRIMER_LEFT_13_GC_PERCENT=39.130
PRIMER_RIGHT_13_GC_PERCENT=39.130
PRIMER_LEFT_13_SELF_ANY=5.00
PRIMER_RIGHT_13_SELF_ANY=5.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=2.00
PRIMER_LEFT_13_MISPRIMING_SCORE=12.00, CER Human D22Z3 repetitive DNA (centromeric DNA)
PRIMER_RIGHT_13_MISPRIMING_SCORE=12.00, MER42a MER42 repetitive sequence: subfamily MER42a - a consensus
PRIMER_PAIR_13_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_13_END_STABILITY=6.4000
PRIMER_RIGHT_13_END_STABILITY=7.3000
PRIMER_PAIR_13_COMPL_ANY=6.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_13=613
PRIMER_PAIR_PENALTY_14=0.1877
PRIMER_LEFT_14_PENALTY=0.146140
PRIMER_RIGHT_14_PENALTY=0.041548
PRIMER_LEFT_14_SEQUENCE=CAAAGGGGGATATGAGACTTTCT
PRIMER_RIGHT_14_SEQUENCE=TAGAGGAAGGGAAGGATTTCAAC
PRIMER_LEFT_14=584,23
PRIMER_RIGHT_14=769,23
PRIMER_LEFT_14_TM=59.854
PRIMER_RIGHT_14_TM=59.958
PRIMER_LEFT_14_GC_PERCENT=43.478
PRIMER_RIGHT_14_GC_PERCENT=43.478
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=3.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_MISPRIMING_SCORE=11.00, L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_RIGHT_14_MISPRIMING_SCORE=11.00, reverse Tigger2 Autonomous DNA transposon
PRIMER_PAIR_14_MISPRIMING_SCORE=20.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_14_END_STABILITY=7.0000
PRIMER_RIGHT_14_END_STABILITY=6.7000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_14=186
PRIMER_PAIR_PENALTY_15=0.1877
PRIMER_LEFT_15_PENALTY=0.146140
PRIMER_RIGHT_15_PENALTY=0.041548
PRIMER_LEFT_15_SEQUENCE=GTCTACTTCCTCAAAGGGGGATA
PRIMER_RIGHT_15_SEQUENCE=TAGAGGAAGGGAAGGATTTCAAC
PRIMER_LEFT_15=573,23
PRIMER_RIGHT_15=769,23
PRIMER_LEFT_15_TM=59.854
PRIMER_RIGHT_15_TM=59.958
PRIMER_LEFT_15_GC_PERCENT=47.826
PRIMER_RIGHT_15_GC_PERCENT=43.478
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=2.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_MISPRIMING_SCORE=11.00, LTR9 LTR from human endogenous retrovirus-like sequence (HUERS-P3)
PRIMER_RIGHT_15_MISPRIMING_SCORE=11.00, reverse Tigger2 Autonomous DNA transposon
PRIMER_PAIR_15_MISPRIMING_SCORE=20.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_15_END_STABILITY=7.1000
PRIMER_RIGHT_15_END_STABILITY=6.7000
PRIMER_PAIR_15_COMPL_ANY=7.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_15=197
PRIMER_PAIR_PENALTY_16=0.1877
PRIMER_LEFT_16_PENALTY=0.146140
PRIMER_RIGHT_16_PENALTY=0.041548
PRIMER_LEFT_16_SEQUENCE=GTCTACTTCCTCAAAGGGGGATA
PRIMER_RIGHT_16_SEQUENCE=GTAGAGGAAGGGAAGGATTTCAA
PRIMER_LEFT_16=573,23
PRIMER_RIGHT_16=770,23
PRIMER_LEFT_16_TM=59.854
PRIMER_RIGHT_16_TM=59.958
PRIMER_LEFT_16_GC_PERCENT=47.826
PRIMER_RIGHT_16_GC_PERCENT=43.478
PRIMER_LEFT_16_SELF_ANY=3.00
PRIMER_RIGHT_16_SELF_ANY=3.00
PRIMER_LEFT_16_SELF_END=2.00
PRIMER_RIGHT_16_SELF_END=3.00
PRIMER_LEFT_16_MISPRIMING_SCORE=11.00, LTR9 LTR from human endogenous retrovirus-like sequence (HUERS-P3)
PRIMER_RIGHT_16_MISPRIMING_SCORE=11.00, MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_PAIR_16_MISPRIMING_SCORE=20.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_16_END_STABILITY=7.1000
PRIMER_RIGHT_16_END_STABILITY=7.3000
PRIMER_PAIR_16_COMPL_ANY=7.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_16=198
PRIMER_PAIR_PENALTY_17=0.1877
PRIMER_LEFT_17_PENALTY=0.146140
PRIMER_RIGHT_17_PENALTY=0.041548
PRIMER_LEFT_17_SEQUENCE=CAAAGGGGGATATGAGACTTTCT
PRIMER_RIGHT_17_SEQUENCE=GTAGAGGAAGGGAAGGATTTCAA
PRIMER_LEFT_17=584,23
PRIMER_RIGHT_17=770,23
PRIMER_LEFT_17_TM=59.854
PRIMER_RIGHT_17_TM=59.958
PRIMER_LEFT_17_GC_PERCENT=43.478
PRIMER_RIGHT_17_GC_PERCENT=43.478
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=3.00
PRIMER_RIGHT_17_SELF_END=3.00
PRIMER_LEFT_17_MISPRIMING_SCORE=11.00, L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_RIGHT_17_MISPRIMING_SCORE=11.00, MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_PAIR_17_MISPRIMING_SCORE=20.00, L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_LEFT_17_END_STABILITY=7.0000
PRIMER_RIGHT_17_END_STABILITY=7.3000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_17=187
PRIMER_PAIR_PENALTY_18=0.1906
PRIMER_LEFT_18_PENALTY=0.176396
PRIMER_RIGHT_18_PENALTY=0.014220
PRIMER_LEFT_18_SEQUENCE=GCATAAGCTGATCACCGTCTAGT
PRIMER_RIGHT_18_SEQUENCE=ACTAAGAATCATGCCAAAGTCCA
PRIMER_LEFT_18=1823,23
PRIMER_RIGHT_18=2030,23
PRIMER_LEFT_18_TM=59.824
PRIMER_RIGHT_18_TM=60.014
PRIMER_LEFT_18_GC_PERCENT=47.826
PRIMER_RIGHT_18_GC_PERCENT=39.130
PRIMER_LEFT_18_SELF_ANY=6.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=2.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_18_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_18_MISPRIMING_SCORE=22.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_18_END_STABILITY=5.4000
PRIMER_RIGHT_18_END_STABILITY=7.9000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_18=208
PRIMER_PAIR_PENALTY_19=0.1994
PRIMER_LEFT_19_PENALTY=0.041548
PRIMER_RIGHT_19_PENALTY=0.157853
PRIMER_LEFT_19_SEQUENCE=TTGAAATCCTTCCCTTCCTCTAC
PRIMER_RIGHT_19_SEQUENCE=TCCATTTGTAGTGTAGGTGGGTC
PRIMER_LEFT_19=748,23
PRIMER_RIGHT_19=891,23
PRIMER_LEFT_19_TM=59.958
PRIMER_RIGHT_19_TM=60.158
PRIMER_LEFT_19_GC_PERCENT=43.478
PRIMER_RIGHT_19_GC_PERCENT=47.826
PRIMER_LEFT_19_SELF_ANY=3.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_MISPRIMING_SCORE=12.00, reverse L1MC2 3'-end of L1 repeat (subfamily L1MC2) - a consensus sequence
PRIMER_RIGHT_19_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_19_MISPRIMING_SCORE=21.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_19_END_STABILITY=5.4000
PRIMER_RIGHT_19_END_STABILITY=9.1000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_19=144
=