PRIMER_SEQUENCE_ID=NM_004420
SEQUENCE=GGGGAGCCGAGGCGAGCGCGAGCGAGGTCCAGCACCATGTGCTAGGTCACTCCCAGCGCGAGGCCACACCTGGGCCGTCGGAGCAGCCCCTCCTCACTTCAGGGGTCACCCTCCCCAGCACCCATTGCCCCACCATGGCTGGGGACCGGCTCCCGAGGAAGGTGATGGATGCCAAGAAGCTGGCCAGCCTGCTGCGGGGCGGGCCTGGGGGGCCGCTGGTCATCGACAGCCGCTCCTTCGTGGAGTACAACAGCTGGCATGTGCTCAGCTCCGTCAACATCTGCTGCTCCAAGCTGGTGAAGCGGCGGCTGCAGCAGGGCAAGGTGACCATTGCGGAGCTCATCCAGCCGGCTGCACGCAGCCAGGTGGAGGCTACGGAGCCACAGGACGTGGTGGTCTATGACCAGAGCACGCGGGACGCCAGCGTGCTGGCCGCAGACAGCTTCCTCTCCATCCTGCTGAGCAAGCTGGACGGCTGCTTCGACAGCGTGGCCATCCTCACTGGGGGCTTCGCCACCTTCTCCTCCTGCTTCCCCGGCCTCTGCGAGGGCAAGCCTGCTGCCCTGCTACCCATGAGCCTCTCCCAGCCCTGCCTGCCTGTGCCCAGCGTGGGCCTGACCCGCATCCTGCCTCACCTCTACCTGGGCTCGCAGAAGGACGTCCTAAACAAGGATCTGATGACGCAAAATGGAATAAGCTACGTCCTCAACGCCAGCAACTCCTGCCCCAAGCCTGACTTCATCTGCGAGAGCCGCTTCATGCGGGTCCCCATCAACGACAACTACTGTGAAAAACTGCTGCCCTGGCTGGACAAGTCCATCGAGTTCATCGATAAAGCCAAGCTCTCCAGCTGCCAAGTCATCGTCCACTGTCTGGCTGGCATCTCCCGCTCTGCCACCATCGCCATCGCCTACATCATGAAGACCATGGGCATGTCCTCCGACGACGCCTACAGGTTCGTGAAGGACAGGCGCCCGTCCATCTCGCCCAACTTCAACTTCCTGGGCCAGCTGCTGGAGTACGAGCGCACGCTGAAGCTGCTGGCCGCCCTGCAGGGCGACCCGGGCACCCCCTCAGGGACGCCGGAGCCTCCGCCCAGTCCTGCCGCCGGGGCCCCGCTGCCACGGCTGCCACCACCTACCTCAGAGAGCGCTGCCACAGGGAATGCGGCTGCCAGGGAGGGCGGCCTGAGCGCGGGCGGGGAGCCCCCCGCGCCCCCCACGCCCCCGGCGACCAGCGCACTGCAGCAGGGCCTGCGCGGCCTGCACCTCTCCTCGGACCGCCTGCAGGACACTAACCGCCTCAAGCGCTCCTTCTCCCTGGACATCAAGTCTGCCTACGCCCCTAGCAGGCGGCCCGACGGCCCCGGGCCCCCCGACCCCGGCGAGGCCCCGAAGCTCTGCAAGCTGGACAGCCCGTCGGGGGCCGCGCTGGGCCTGTCCTCGCCCAGCCCGGACAGCCCGGACGCCGCGCCTGAGGCGCGCCCACGGCCCCGCCGGCGGCCCCGGCCCCCCGCCGGCTCCCCCGCGCGCTCCCCCGCGCACAGCCTCGGCCTGAACTTCGGCGATGCGGCCCGGCAGACTCCGCGGCACGGCCTCTCGGCCCTGTCGGCGCCCGGGCTGCCCGGCCCTGGCCAGCCGGCCGGCCCCGGGGCCTGGGCACCGCCGCTTGACTCCCCAGGCACGCCGTCGCCCGACGGGCCCTGGTGCTTCAGCCCCGAGGGCGCACAGGGGGCGGGCGGGGTGCTGTTTGCGCCCTTCGGCCGGGCGGGCGCCCCGGGACCAGGCGGCGGCAGCGACCTGCGGCGGCGGGAGGCAGCGAGGGCTGAGCCCCGGGACGCGCGGACCGGCTGGCCCGAGGAGCCGGCCCCGGAGACGCAGTTCAAGCGCCGCAGCTGCCAGATGGAGTTCGAGGAGGGCATGGTGGAGGGGCGCGCGCGCGGCGAGGAGCTGGCCGCCCTGGGCAAGCAGGCGAGCTTCTCGGGCAGCGTGGAGGTCATCGAGGTGTCCTGACCCCTCCGCTGCCCTCGGCCCCGCCGCCCGCAGCCAGGCCCGTTATAAATGTATATTATATATAATGCAAAGAAAGGTAAATGGTTTTACTGGGATTTTTATCGAGAAGTAAATATTTCGATTTTTTATTTATTTAAGCTGTTCATTCTGGCAATGATTTGGCAACAGTGCGGGTGGTCCTCGAGCTCTATTTTTACTGTCTGGTATTTAAACTGAAACATACGTTTCTAAGCAATACGAGGCCACCTTCAGTCGCAAGCTGGGTGCCAGGCCTGGGGCCCTCCCAGTTCCCCCGCCCCAGGAAACACTGCTGACCTTTGCAAAGGCTGCCGAGCTTTCGTGCACTTTTTACATAACAAAAAGGG
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 27261, GC content failed 6893, low tm 3797, high tm 13341, high any compl 12, high end compl 14, high repeat similarity 34, long poly-x seq 18, ok 3152
PRIMER_RIGHT_EXPLAIN=considered 27275, GC content failed 6902, low tm 3917, high tm 13184, high end compl 8, high repeat similarity 6, long poly-x seq 16, ok 3242
PRIMER_PAIR_EXPLAIN=considered 870, unacceptable product size 817, high end compl 6, ok 47
PRIMER_PAIR_PENALTY=0.0870
PRIMER_LEFT_PENALTY=0.005764
PRIMER_RIGHT_PENALTY=0.081257
PRIMER_LEFT_SEQUENCE=ATGACGCAAAATGGAATAAGCTA
PRIMER_RIGHT_SEQUENCE=TTCACAGTAGTTGTCGTTGATGG
PRIMER_LEFT=677,23
PRIMER_RIGHT=790,23
PRIMER_LEFT_TM=60.006
PRIMER_RIGHT_TM=60.081
PRIMER_LEFT_GC_PERCENT=34.783
PRIMER_RIGHT_GC_PERCENT=43.478
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_MISPRIMING_SCORE=12.00, MER6 Nonautonomous DNA transposon
PRIMER_RIGHT_MISPRIMING_SCORE=11.00, MER34 a consensus
PRIMER_PAIR_MISPRIMING_SCORE=23.00, MER34 a consensus
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=114
PRIMER_PAIR_PENALTY_1=0.1315
PRIMER_LEFT_1_PENALTY=0.005764
PRIMER_RIGHT_1_PENALTY=0.125755
PRIMER_LEFT_1_SEQUENCE=ATGACGCAAAATGGAATAAGCTA
PRIMER_RIGHT_1_SEQUENCE=GGCAGCAGTTTTTCACAGTAGTT
PRIMER_LEFT_1=677,23
PRIMER_RIGHT_1=801,23
PRIMER_LEFT_1_TM=60.006
PRIMER_RIGHT_1_TM=59.874
PRIMER_LEFT_1_GC_PERCENT=34.783
PRIMER_RIGHT_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_MISPRIMING_SCORE=12.00, MER6 Nonautonomous DNA transposon
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_PAIR_1_MISPRIMING_SCORE=23.00, L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=5.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=125
PRIMER_PAIR_PENALTY_2=0.2944
PRIMER_LEFT_2_PENALTY=0.141011
PRIMER_RIGHT_2_PENALTY=0.153359
PRIMER_LEFT_2_SEQUENCE=ATTTAAGCTGTTCATTCTGGCAA
PRIMER_RIGHT_2_SEQUENCE=ATGTAAAAAGTGCACGAAAGCTC
PRIMER_LEFT_2=2143,23
PRIMER_RIGHT_2=2365,23
PRIMER_LEFT_2_TM=60.141
PRIMER_RIGHT_2_TM=59.847
PRIMER_LEFT_2_GC_PERCENT=34.783
PRIMER_RIGHT_2_GC_PERCENT=39.130
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_2_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_2_MISPRIMING_SCORE=24.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_2_END_STABILITY=10.0000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=223
PRIMER_PAIR_PENALTY_3=0.2944
PRIMER_LEFT_3_PENALTY=0.141011
PRIMER_RIGHT_3_PENALTY=0.153359
PRIMER_LEFT_3_SEQUENCE=TTTAAGCTGTTCATTCTGGCAAT
PRIMER_RIGHT_3_SEQUENCE=ATGTAAAAAGTGCACGAAAGCTC
PRIMER_LEFT_3=2144,23
PRIMER_RIGHT_3=2365,23
PRIMER_LEFT_3_TM=60.141
PRIMER_RIGHT_3_TM=59.847
PRIMER_LEFT_3_GC_PERCENT=34.783
PRIMER_RIGHT_3_GC_PERCENT=39.130
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, reverse MER4C a consensus
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_3_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=222
PRIMER_PAIR_PENALTY_4=0.3065
PRIMER_LEFT_4_PENALTY=0.141011
PRIMER_RIGHT_4_PENALTY=0.165480
PRIMER_LEFT_4_SEQUENCE=ATTTAAGCTGTTCATTCTGGCAA
PRIMER_RIGHT_4_SEQUENCE=GCCTCGTATTGCTTAGAAACGTA
PRIMER_LEFT_4=2143,23
PRIMER_RIGHT_4=2254,23
PRIMER_LEFT_4_TM=60.141
PRIMER_RIGHT_4_TM=59.835
PRIMER_LEFT_4_GC_PERCENT=34.783
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_4_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_4_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_4_END_STABILITY=10.0000
PRIMER_RIGHT_4_END_STABILITY=7.1000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=112
PRIMER_PAIR_PENALTY_5=0.3188
PRIMER_LEFT_5_PENALTY=0.165480
PRIMER_RIGHT_5_PENALTY=0.153359
PRIMER_LEFT_5_SEQUENCE=TACGTTTCTAAGCAATACGAGGC
PRIMER_RIGHT_5_SEQUENCE=ATGTAAAAAGTGCACGAAAGCTC
PRIMER_LEFT_5=2232,23
PRIMER_RIGHT_5=2365,23
PRIMER_LEFT_5_TM=59.835
PRIMER_RIGHT_5_TM=59.847
PRIMER_LEFT_5_GC_PERCENT=43.478
PRIMER_RIGHT_5_GC_PERCENT=39.130
PRIMER_LEFT_5_SELF_ANY=5.00
PRIMER_RIGHT_5_SELF_ANY=6.00
PRIMER_LEFT_5_SELF_END=2.00
PRIMER_RIGHT_5_SELF_END=2.00
PRIMER_LEFT_5_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_5_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_5_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_5_END_STABILITY=9.4000
PRIMER_RIGHT_5_END_STABILITY=7.9000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_5=134
PRIMER_PAIR_PENALTY_6=0.8515
PRIMER_LEFT_6_PENALTY=0.698131
PRIMER_RIGHT_6_PENALTY=0.153359
PRIMER_LEFT_6_SEQUENCE=TGGTTTTACTGGGATTTTTATCG
PRIMER_RIGHT_6_SEQUENCE=ATGTAAAAAGTGCACGAAAGCTC
PRIMER_LEFT_6=2093,23
PRIMER_RIGHT_6=2365,23
PRIMER_LEFT_6_TM=59.302
PRIMER_RIGHT_6_TM=59.847
PRIMER_LEFT_6_GC_PERCENT=34.783
PRIMER_RIGHT_6_GC_PERCENT=39.130
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=2.00
PRIMER_RIGHT_6_SELF_END=2.00
PRIMER_LEFT_6_MISPRIMING_SCORE=12.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_6_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_6_MISPRIMING_SCORE=24.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_6_END_STABILITY=7.6000
PRIMER_RIGHT_6_END_STABILITY=7.9000
PRIMER_PAIR_6_COMPL_ANY=6.00
PRIMER_PAIR_6_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_6=273
PRIMER_PAIR_PENALTY_7=0.8636
PRIMER_LEFT_7_PENALTY=0.698131
PRIMER_RIGHT_7_PENALTY=0.165480
PRIMER_LEFT_7_SEQUENCE=TGGTTTTACTGGGATTTTTATCG
PRIMER_RIGHT_7_SEQUENCE=GCCTCGTATTGCTTAGAAACGTA
PRIMER_LEFT_7=2093,23
PRIMER_RIGHT_7=2254,23
PRIMER_LEFT_7_TM=59.302
PRIMER_RIGHT_7_TM=59.835
PRIMER_LEFT_7_GC_PERCENT=34.783
PRIMER_RIGHT_7_GC_PERCENT=43.478
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=5.00
PRIMER_LEFT_7_SELF_END=2.00
PRIMER_RIGHT_7_SELF_END=3.00
PRIMER_LEFT_7_MISPRIMING_SCORE=12.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_7_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_7_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_7_END_STABILITY=7.6000
PRIMER_RIGHT_7_END_STABILITY=7.1000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_7=162
PRIMER_PAIR_PENALTY_8=0.8843
PRIMER_LEFT_8_PENALTY=0.803017
PRIMER_RIGHT_8_PENALTY=0.081257
PRIMER_LEFT_8_SEQUENCE=AAATGGAATAAGCTACGTCCTCA
PRIMER_RIGHT_8_SEQUENCE=TTCACAGTAGTTGTCGTTGATGG
PRIMER_LEFT_8=685,23
PRIMER_RIGHT_8=790,23
PRIMER_LEFT_8_TM=59.197
PRIMER_RIGHT_8_TM=60.081
PRIMER_LEFT_8_GC_PERCENT=39.130
PRIMER_RIGHT_8_GC_PERCENT=43.478
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=2.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_8_MISPRIMING_SCORE=11.00, MER34 a consensus
PRIMER_PAIR_8_MISPRIMING_SCORE=23.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_8_END_STABILITY=8.2000
PRIMER_RIGHT_8_END_STABILITY=8.1000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_8=106
PRIMER_PAIR_PENALTY_9=0.9288
PRIMER_LEFT_9_PENALTY=0.803017
PRIMER_RIGHT_9_PENALTY=0.125755
PRIMER_LEFT_9_SEQUENCE=AAATGGAATAAGCTACGTCCTCA
PRIMER_RIGHT_9_SEQUENCE=GGCAGCAGTTTTTCACAGTAGTT
PRIMER_LEFT_9=685,23
PRIMER_RIGHT_9=801,23
PRIMER_LEFT_9_TM=59.197
PRIMER_RIGHT_9_TM=59.874
PRIMER_LEFT_9_GC_PERCENT=39.130
PRIMER_RIGHT_9_GC_PERCENT=43.478
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=2.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_PAIR_9_MISPRIMING_SCORE=23.00, L1MB7 3'-end of L1 repeat (subfamily L1MB7) - a consensus sequence
PRIMER_LEFT_9_END_STABILITY=8.2000
PRIMER_RIGHT_9_END_STABILITY=5.7000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_9=117
PRIMER_PAIR_PENALTY_10=0.9974
PRIMER_LEFT_10_PENALTY=0.005764
PRIMER_RIGHT_10_PENALTY=0.991617
PRIMER_LEFT_10_SEQUENCE=ATGACGCAAAATGGAATAAGCTA
PRIMER_RIGHT_10_SEQUENCE=GCAGTTTTTCACAGTAGTTGTCG
PRIMER_LEFT_10=677,23
PRIMER_RIGHT_10=797,23
PRIMER_LEFT_10_TM=60.006
PRIMER_RIGHT_10_TM=59.008
PRIMER_LEFT_10_GC_PERCENT=34.783
PRIMER_RIGHT_10_GC_PERCENT=43.478
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=2.00
PRIMER_RIGHT_10_SELF_END=2.00
PRIMER_LEFT_10_MISPRIMING_SCORE=12.00, MER6 Nonautonomous DNA transposon
PRIMER_RIGHT_10_MISPRIMING_SCORE=12.00, L1MA2 3'-end of L1 repeat (subfamily L1MA2) - a consensus sequence
PRIMER_PAIR_10_MISPRIMING_SCORE=23.00, L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_LEFT_10_END_STABILITY=7.2000
PRIMER_RIGHT_10_END_STABILITY=8.4000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_10=121
PRIMER_PAIR_PENALTY_11=1.0346
PRIMER_LEFT_11_PENALTY=0.881284
PRIMER_RIGHT_11_PENALTY=0.153359
PRIMER_LEFT_11_SEQUENCE=TGCAAAGAAAGGTAAATGGTTTT
PRIMER_RIGHT_11_SEQUENCE=ATGTAAAAAGTGCACGAAAGCTC
PRIMER_LEFT_11=2077,23
PRIMER_RIGHT_11=2365,23
PRIMER_LEFT_11_TM=59.119
PRIMER_RIGHT_11_TM=59.847
PRIMER_LEFT_11_GC_PERCENT=30.435
PRIMER_RIGHT_11_GC_PERCENT=39.130
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=6.00
PRIMER_LEFT_11_SELF_END=2.00
PRIMER_RIGHT_11_SELF_END=2.00
PRIMER_LEFT_11_MISPRIMING_SCORE=12.00, L1MA5 3'-end of L1 repeat (subfamily L1MA5) - a consensus sequence
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_11_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_11_END_STABILITY=7.0000
PRIMER_RIGHT_11_END_STABILITY=7.9000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_11=289
PRIMER_PAIR_PENALTY_12=1.0468
PRIMER_LEFT_12_PENALTY=0.881284
PRIMER_RIGHT_12_PENALTY=0.165480
PRIMER_LEFT_12_SEQUENCE=TGCAAAGAAAGGTAAATGGTTTT
PRIMER_RIGHT_12_SEQUENCE=GCCTCGTATTGCTTAGAAACGTA
PRIMER_LEFT_12=2077,23
PRIMER_RIGHT_12=2254,23
PRIMER_LEFT_12_TM=59.119
PRIMER_RIGHT_12_TM=59.835
PRIMER_LEFT_12_GC_PERCENT=30.435
PRIMER_RIGHT_12_GC_PERCENT=43.478
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=5.00
PRIMER_LEFT_12_SELF_END=2.00
PRIMER_RIGHT_12_SELF_END=3.00
PRIMER_LEFT_12_MISPRIMING_SCORE=12.00, L1MA5 3'-end of L1 repeat (subfamily L1MA5) - a consensus sequence
PRIMER_RIGHT_12_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_12_MISPRIMING_SCORE=20.00, MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_LEFT_12_END_STABILITY=7.0000
PRIMER_RIGHT_12_END_STABILITY=7.1000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_12=178
PRIMER_PAIR_PENALTY_13=1.0836
PRIMER_LEFT_13_PENALTY=1.002310
PRIMER_RIGHT_13_PENALTY=0.081257
PRIMER_LEFT_13_SEQUENCE=GGACGTCCTAAACAAGGATCTG
PRIMER_RIGHT_13_SEQUENCE=TTCACAGTAGTTGTCGTTGATGG
PRIMER_LEFT_13=655,22
PRIMER_RIGHT_13=790,23
PRIMER_LEFT_13_TM=59.998
PRIMER_RIGHT_13_TM=60.081
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=43.478
PRIMER_LEFT_13_SELF_ANY=8.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_13_MISPRIMING_SCORE=11.00, MER34 a consensus
PRIMER_PAIR_13_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_13_END_STABILITY=6.6000
PRIMER_RIGHT_13_END_STABILITY=8.1000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_13=136
PRIMER_PAIR_PENALTY_14=1.1281
PRIMER_LEFT_14_PENALTY=1.002310
PRIMER_RIGHT_14_PENALTY=0.125755
PRIMER_LEFT_14_SEQUENCE=GGACGTCCTAAACAAGGATCTG
PRIMER_RIGHT_14_SEQUENCE=GGCAGCAGTTTTTCACAGTAGTT
PRIMER_LEFT_14=655,22
PRIMER_RIGHT_14=801,23
PRIMER_LEFT_14_TM=59.998
PRIMER_RIGHT_14_TM=59.874
PRIMER_LEFT_14_GC_PERCENT=50.000
PRIMER_RIGHT_14_GC_PERCENT=43.478
PRIMER_LEFT_14_SELF_ANY=8.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_14_MISPRIMING_SCORE=12.00, L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_PAIR_14_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_14_END_STABILITY=6.6000
PRIMER_RIGHT_14_END_STABILITY=5.7000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_14=147
PRIMER_PAIR_PENALTY_15=1.1941
PRIMER_LEFT_15_PENALTY=1.068375
PRIMER_RIGHT_15_PENALTY=0.125755
PRIMER_LEFT_15_SEQUENCE=AATGGAATAAGCTACGTCCTCAAC
PRIMER_RIGHT_15_SEQUENCE=GGCAGCAGTTTTTCACAGTAGTT
PRIMER_LEFT_15=686,24
PRIMER_RIGHT_15=801,23
PRIMER_LEFT_15_TM=59.932
PRIMER_RIGHT_15_TM=59.874
PRIMER_LEFT_15_GC_PERCENT=41.667
PRIMER_RIGHT_15_GC_PERCENT=43.478
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_15_MISPRIMING_SCORE=12.00, L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_PAIR_15_MISPRIMING_SCORE=22.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_15_END_STABILITY=6.7000
PRIMER_RIGHT_15_END_STABILITY=5.7000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_15=116
PRIMER_PAIR_PENALTY_16=1.2108
PRIMER_LEFT_16_PENALTY=0.005764
PRIMER_RIGHT_16_PENALTY=1.205063
PRIMER_LEFT_16_SEQUENCE=ATGACGCAAAATGGAATAAGCTA
PRIMER_RIGHT_16_SEQUENCE=GCAGTTTTTCACAGTAGTTGTCGT
PRIMER_LEFT_16=677,23
PRIMER_RIGHT_16=797,24
PRIMER_LEFT_16_TM=60.006
PRIMER_RIGHT_16_TM=59.795
PRIMER_LEFT_16_GC_PERCENT=34.783
PRIMER_RIGHT_16_GC_PERCENT=41.667
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=3.00
PRIMER_LEFT_16_SELF_END=2.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_MISPRIMING_SCORE=12.00, MER6 Nonautonomous DNA transposon
PRIMER_RIGHT_16_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_16_MISPRIMING_SCORE=23.00, MER34 a consensus
PRIMER_LEFT_16_END_STABILITY=7.2000
PRIMER_RIGHT_16_END_STABILITY=7.8000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_16=121
PRIMER_PAIR_PENALTY_17=1.2241
PRIMER_LEFT_17_PENALTY=1.070750
PRIMER_RIGHT_17_PENALTY=0.153359
PRIMER_LEFT_17_SEQUENCE=ATACGTTTCTAAGCAATACGAGGC
PRIMER_RIGHT_17_SEQUENCE=ATGTAAAAAGTGCACGAAAGCTC
PRIMER_LEFT_17=2231,24
PRIMER_RIGHT_17=2365,23
PRIMER_LEFT_17_TM=60.071
PRIMER_RIGHT_17_TM=59.847
PRIMER_LEFT_17_GC_PERCENT=41.667
PRIMER_RIGHT_17_GC_PERCENT=39.130
PRIMER_LEFT_17_SELF_ANY=5.00
PRIMER_RIGHT_17_SELF_ANY=6.00
PRIMER_LEFT_17_SELF_END=2.00
PRIMER_RIGHT_17_SELF_END=2.00
PRIMER_LEFT_17_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_17_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_17_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_17_END_STABILITY=9.4000
PRIMER_RIGHT_17_END_STABILITY=7.9000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_17=135
PRIMER_PAIR_PENALTY_18=1.2638
PRIMER_LEFT_18_PENALTY=1.110393
PRIMER_RIGHT_18_PENALTY=0.153359
PRIMER_LEFT_18_SEQUENCE=TTTAAGCTGTTCATTCTGGCAA
PRIMER_RIGHT_18_SEQUENCE=ATGTAAAAAGTGCACGAAAGCTC
PRIMER_LEFT_18=2144,22
PRIMER_RIGHT_18=2365,23
PRIMER_LEFT_18_TM=59.890
PRIMER_RIGHT_18_TM=59.847
PRIMER_LEFT_18_GC_PERCENT=36.364
PRIMER_RIGHT_18_GC_PERCENT=39.130
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=6.00
PRIMER_LEFT_18_SELF_END=2.00
PRIMER_RIGHT_18_SELF_END=2.00
PRIMER_LEFT_18_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_18_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_18_MISPRIMING_SCORE=24.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_18_END_STABILITY=10.0000
PRIMER_RIGHT_18_END_STABILITY=7.9000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_18=222
PRIMER_PAIR_PENALTY_19=1.2749
PRIMER_LEFT_19_PENALTY=1.149184
PRIMER_RIGHT_19_PENALTY=0.125755
PRIMER_LEFT_19_SEQUENCE=GGAATAAGCTACGTCCTCAACG
PRIMER_RIGHT_19_SEQUENCE=GGCAGCAGTTTTTCACAGTAGTT
PRIMER_LEFT_19=689,22
PRIMER_RIGHT_19=801,23
PRIMER_LEFT_19_TM=60.149
PRIMER_RIGHT_19_TM=59.874
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=43.478
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=2.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_19_MISPRIMING_SCORE=12.00, L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_PAIR_19_MISPRIMING_SCORE=22.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_19_END_STABILITY=8.7000
PRIMER_RIGHT_19_END_STABILITY=5.7000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_19=113
=