PRIMER_SEQUENCE_ID=NM_004685
SEQUENCE=CAGTACCGGCCTCCTCCAGCAGGGGGCGCTGTGGGATCCGCCGCTGTCCGGGACGAACCAGCAAGCCGCTCTCGGTCCACACACGCTCTGTCTGCCTGCCCCGAGTCCCCCGGGAGGCCGCGGGGTTTGGGGAAGTGTTTCTAGGAGACGGCGCTCACCGGCTGCACCTGCGCCGTTGACGCCACCGGGGCCGGCAGACAGACCCGCGGCGCTGGCTGGTGGAGGGAGTTCCCGCTTGCTCTCTGTCGCTGTCACCGCCCTGTTTCTGTAGCCGTATGGTACGCCTGTGAGACCGGCTGCCGGCTGACGTCTCCTTGCGATGGAGCATATCCGGACGACCAAGGTCGAACAAGTAAAATTACTTGACCGATTCAGTACCAGCAACAAGTCATTAACAGGAACACTGTATCTTACGGCTACACATCTATTATTTATCGACTCTCATCAAAAAGAAACCTGGATATTACACCACCATATTGCCTCAGTAGAGAAACTTGCTTTGACTACTTCTGGATGCCCCCTTGTGATACAGTGCAAGAACTTCAGAACTGTGCATTTCATTGTTCCCAGAGAAAGAGATTGCCATGATATTTACAACTCTTTGCTACAACTGTCAAAACAAGCAAAATATGAAGATCTCTATGCATTTTCTTATAATCCCAAACAAAATGATTCAGAACGACTACAAGGCTGGCAGCTCATTGATCTCGCTGAGGAATATAAGAGGATGGGAGTGCCAAACTCACACTGGCAGTTGTCTGATGCCAACCGGGACTACAAGATTTGTGAAACTTACCCCAGAGAACTTTATGTTCCCCGGATAGCAAGCAAACCAATAATTGTTGGTAGTTCCAAGTTCCGGAGCAAGGGAAGATTCCCAGTTCTTTCCTACTATCATCAAGATAAGGAGGCTGCCATTTGTCGATGTAGTCAGCCACTCTCTGGATTCAGTGCCAGGTGCCTGGAGGATGAACATTTGCTTCAAGCCATTAGTAAAGCCAATCCAGTCAATCGCTATATGTACGTCATGGATACCAGGCCAAAACTGAATGCAATGGCCAACAGAGCAGCTGGAAAAGGTTATGAAAATGAAGACAACTATTCCAATATTAGATTTCAGTTTGTTGGAATTGAAAATATTCATGTCATGAGGTCCAGCCTTCAGAAATTATTGGAAGTCAATGGCACTAAAGGGCTTTCTGTCAATGATTTCTACTCCGGTTTGGAGAGCTCGGGATGGCTTCGCCATATCAAAGCTGTTATGGATGCTGCAGTCTTCTTGGCCAAAGCAATAACAGTTGAAAATGCAAGTGTGTTGGTGCATTGTTCCGATGGTTGGGATAGGACTTCCCAGGTTTGTTCCCTGGGTTCTCTTTTATTGGATTCCTACTACAGGACAATCAAAGGATTCATGGTTTTAATAGAAAAGGATTGGATCTCTTTTGGACATAAATTTTCAGAGAGGTGTGGCCAGTTGGATGGTGACCCAAAGGAAGTCTCACCAGTGTTTACTCAGTTCTTGGAATGTGTGTGGCATTTGACCGAACAGTTTCCACAAGCCTTTGAATTCAGTGAAGCATTTCTTCTTCAGATCCATGAGCATATTCATTCATGCCAGTTTGGAAACTTCCTTGGAAATTGTCAGAAGGAAAGAGAAGAGCTCAAGTTGAAGGAGAAGACTTATTCCCTGTGGCCATTTCTTTTGGAAGACCAAAAGAAGTACTTAAATCCTCTCTACAGTTCCGAATCTCACAGATTTACAGTTTTGGAGCCAAATACAGTATCTTTCAATTTTAAGTTTTGGAGGAACATGTACCATCAATTTGATCGAACACTGCATCCTAGGCAGTCTGTATTTAATATAATTATGAATATGAATGAGCAAAATAAACAATTAGAGAAAGATATTAAAGACCTAGAATCTAAAATTAAACAACGCAAAAATAAGCAAACAGATGGCATCCTCACCAAGGAATTGTTACATTCAGTTCATCCTGAATCACCTAACCTCAAAACTTCCCTGTGTTTTAAAGAGCAGACTCTGCTACCCGTAAATGATGCTCTTCGAACTATAGAGGGCAGCAGCCCGGCAGATAATCGTTATAGTGAATATGCAGAAGAGTTTTCTAAATCAGAACCTGCTGTGGTCAGCTTAGAGTATGGTGTGGCAAGAATGACTTGTTAGACTCATAGAGTTTTTTCTGCAATGATTGCAGTACAAGAAAAGGATTATTGTGAGGATGGTCTGTAAGCATAACCAAAAGGAATTTGTCTAATAACAATTTTAGGGTTTAACAGTAGGCTAATAGTTGAAGGAAGGATAATAACTACCCTTGTGAGAGAAATAAGTCATTTTAATTGCATTTCCAGCAAGGAATGACATTCAGTTCTGTAAGAAATGAGTGGTATTTGATGTATTTACTCAAAACACAATTTGCACTGTACACTAGTGAATTGACGTTTATGATTTATGTTAATTCAGCCAAACATAAATAACCTTCCTTAAGTACAATTTAACTTCAAGAAAACAAAATTTGACAACATAGTTTCTTAATAAATGATATGGCATGTACTTTCAATTATGTAGCTTTGTAACTATGAATATTTACATATTTTGCCTTTTAGTGATATTTAATGTTAAAGTGCCATGAAAAATATTTCTAAGAAAGCCTTAAATTCCCAGTGGATTCTTTACCCTTAAGTTTTACAGCCTACAACAAGATTTTTTGTTTTGTTTTTCTTCTGGTCAGCCTTGTTTTGTTTGTAAAGAATTGTGCTCTCATTACTGCTGGGGGTGCATGCTACAATACTTCTATATAAACACTTGTAGAAGTACACTGTTCACGTTTAGCCTGCCCCACTTTTGTATTCAAAATTAATGAAACTGAAGGTTTATTCTGATCATAATTTGTTTAGTGCTACATTTGATAATTTATTATTTACAGCTTAGAATATTGATTTCTTGAATACGTATAAGCACATTTGACTGTCTTTTATATATGGATTACTGCATTCCATTGATTCTTATTTTGTGGTTGGCTTTATTTCTTTCACAACTGTGTAAGTTTAAAGAGCTAAAGCTCTAAAACTGTTCTGAGAAACAATGAATAGTACATGTATATGTATATTTTTAAACTGCCTTATTGCTCAATGAGTTGCTGTTCCTGGAGTATCTAACTTTCAGTTTACTCAAAAACTTTTGGGTAAATAAAAAAGGGAAGTACAGATAGTTTAGGTTGTGCATGGTTGCATGAATTTTGAAGTCATTTCTATGTGGAGCATTATTTTTCTTCTTGTTAAATATAGAAAAAAAAAGAGATTCTAGTACAAAGTTACTGTTTAACAAAAGCAACATAAACTCTGGGAAAGATTTCATTTTGCCATGTTATATTTACTGTTTATTCTGTGTACTAGTACATATCTTTAAATTACCAAAAAACAAGAAACAAAACATAAAAACCCCAAAACTATCACTTGGAATTAGCAATATCACCCAACTGGCTTTAAAATTGAAAATTTAAATAACATGGTGGCATGAAGTACAATTCGAGTATTAGGCAATTTGCATAGTGTTCCTCATGCTACTTTCTGTTACACCTCTATTATATTAGTTTTGAATATAAACATCTTTTTCAGACCAAAAAAAACTTTATTGTATGAGAGCTTATCTTATCCTGTTTATTTTTCCAATGCTTTTCTGTAATACATTATGTAATTTAAAAAATATTCCTTTTTAAACAGCAACAGAAATGCACTATAAAATATAGTATGTGATTAACCAATCCTGCTTCCATATTTAAGCACTGGGAATGGAAACTTAATCTCTGTGACTACAAAGGGAAGTTTTTGTGCCTTGGTGTTCCAGTCACTGATTGTGGTTTTAGAATCTTCTGTGGCTGACTTCTTGTATTCAACTCTGGTTTATTATCTTAATCTTCAACTTCAGACATATATTTGTGTGTTTATATGCTCACAGGTGGACTGAGAAATCAGTTACATCTTAAGTGACCTACAGGGTATATGTTGGCAAAAGCAGACTGTGTATATGTCTTATAAAGTTGAATTTATGTTCAGTGTGTTTGGAAGTGTATAGCATGTAAATTATTTCATATATGATTTAAAGGTAATTAAATGTTCACATTTTACTTTGAATGTTTTTCTTCTGGATAAAACAAATGGAATCAAAAAAAAAAAAAAAAAA
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 48352, GC content failed 3432, low tm 28517, high tm 7391, high any compl 6, high end compl 24, high repeat similarity 48, long poly-x seq 123, ok 8811
PRIMER_RIGHT_EXPLAIN=considered 48316, GC content failed 3754, low tm 28928, high tm 6663, high any compl 1, high end compl 8, high repeat similarity 14, long poly-x seq 126, ok 8822
PRIMER_PAIR_EXPLAIN=considered 1325, unacceptable product size 1256, high end compl 1, ok 68
PRIMER_PAIR_PENALTY=0.0078
PRIMER_LEFT_PENALTY=0.002035
PRIMER_RIGHT_PENALTY=0.005764
PRIMER_LEFT_SEQUENCE=ACCCCAAAACTATCACTTGGAAT
PRIMER_RIGHT_SEQUENCE=ATGCAAATTGCCTAATACTCGAA
PRIMER_LEFT=3458,23
PRIMER_RIGHT=3567,23
PRIMER_LEFT_TM=59.998
PRIMER_RIGHT_TM=60.006
PRIMER_LEFT_GC_PERCENT=39.130
PRIMER_RIGHT_GC_PERCENT=34.783
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_MISPRIMING_SCORE=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=23.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=8.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=110
PRIMER_PAIR_PENALTY_1=0.0158
PRIMER_LEFT_1_PENALTY=0.013803
PRIMER_RIGHT_1_PENALTY=0.002035
PRIMER_LEFT_1_SEQUENCE=AAGAAAGCCTTAAATTCCCAGTG
PRIMER_RIGHT_1_SEQUENCE=ATTCCAAGTGATAGTTTTGGGGT
PRIMER_LEFT_1=2662,23
PRIMER_RIGHT_1=3480,23
PRIMER_LEFT_1_TM=60.014
PRIMER_RIGHT_1_TM=59.998
PRIMER_LEFT_1_GC_PERCENT=39.130
PRIMER_RIGHT_1_GC_PERCENT=39.130
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_MISPRIMING_SCORE=11.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_1_MISPRIMING_SCORE=23.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=10.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=819
PRIMER_PAIR_PENALTY_2=0.0196
PRIMER_LEFT_2_PENALTY=0.013803
PRIMER_RIGHT_2_PENALTY=0.005764
PRIMER_LEFT_2_SEQUENCE=AAGAAAGCCTTAAATTCCCAGTG
PRIMER_RIGHT_2_SEQUENCE=ATGCAAATTGCCTAATACTCGAA
PRIMER_LEFT_2=2662,23
PRIMER_RIGHT_2=3567,23
PRIMER_LEFT_2_TM=60.014
PRIMER_RIGHT_2_TM=60.006
PRIMER_LEFT_2_GC_PERCENT=39.130
PRIMER_RIGHT_2_GC_PERCENT=34.783
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_MISPRIMING_SCORE=11.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_2_MISPRIMING_SCORE=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_2_MISPRIMING_SCORE=22.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=906
PRIMER_PAIR_PENALTY_3=0.0219
PRIMER_LEFT_3_PENALTY=0.006308
PRIMER_RIGHT_3_PENALTY=0.015613
PRIMER_LEFT_3_SEQUENCE=GAAACCTGGATATTACACCACCA
PRIMER_RIGHT_3_SEQUENCE=ATAATTTCTGAAGGCTGGACCTC
PRIMER_LEFT_3=451,23
PRIMER_RIGHT_3=1171,23
PRIMER_LEFT_3_TM=59.994
PRIMER_RIGHT_3_TM=59.984
PRIMER_LEFT_3_GC_PERCENT=43.478
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, MLT1b (MLT1b subfamily) - consensus sequence
PRIMER_PAIR_3_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=721
PRIMER_PAIR_PENALTY_4=0.0435
PRIMER_LEFT_4_PENALTY=0.008825
PRIMER_RIGHT_4_PENALTY=0.034654
PRIMER_LEFT_4_SEQUENCE=AGGAGAAGACTTATTCCCTGTGG
PRIMER_RIGHT_4_SEQUENCE=CATCATTTACGGGTAGCAGAGTC
PRIMER_LEFT_4=1670,23
PRIMER_RIGHT_4=2059,23
PRIMER_LEFT_4_TM=60.009
PRIMER_RIGHT_4_TM=60.035
PRIMER_LEFT_4_GC_PERCENT=47.826
PRIMER_RIGHT_4_GC_PERCENT=47.826
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_MISPRIMING_SCORE=11.00, reverse MER4A a consensus
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_4_MISPRIMING_SCORE=21.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=390
PRIMER_PAIR_PENALTY_5=0.0503
PRIMER_LEFT_5_PENALTY=0.015613
PRIMER_RIGHT_5_PENALTY=0.034654
PRIMER_LEFT_5_SEQUENCE=GAGGTCCAGCCTTCAGAAATTAT
PRIMER_RIGHT_5_SEQUENCE=CATCATTTACGGGTAGCAGAGTC
PRIMER_LEFT_5=1149,23
PRIMER_RIGHT_5=2059,23
PRIMER_LEFT_5_TM=59.984
PRIMER_RIGHT_5_TM=60.035
PRIMER_LEFT_5_GC_PERCENT=43.478
PRIMER_RIGHT_5_GC_PERCENT=47.826
PRIMER_LEFT_5_SELF_ANY=5.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=3.00
PRIMER_RIGHT_5_SELF_END=3.00
PRIMER_LEFT_5_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_5_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_5_MISPRIMING_SCORE=22.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_5_END_STABILITY=5.8000
PRIMER_RIGHT_5_END_STABILITY=6.1000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_5=911
PRIMER_PAIR_PENALTY_6=0.0567
PRIMER_LEFT_6_PENALTY=0.041057
PRIMER_RIGHT_6_PENALTY=0.015613
PRIMER_LEFT_6_SEQUENCE=GTCGATGTAGTCAGCCACTCTCT
PRIMER_RIGHT_6_SEQUENCE=ATAATTTCTGAAGGCTGGACCTC
PRIMER_LEFT_6=920,23
PRIMER_RIGHT_6=1171,23
PRIMER_LEFT_6_TM=59.959
PRIMER_RIGHT_6_TM=59.984
PRIMER_LEFT_6_GC_PERCENT=52.174
PRIMER_RIGHT_6_GC_PERCENT=43.478
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=5.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=2.00
PRIMER_LEFT_6_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_6_MISPRIMING_SCORE=12.00, MLT1b (MLT1b subfamily) - consensus sequence
PRIMER_PAIR_6_MISPRIMING_SCORE=22.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_6_END_STABILITY=6.4000
PRIMER_RIGHT_6_END_STABILITY=7.6000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_6=252
PRIMER_PAIR_PENALTY_7=0.0667
PRIMER_LEFT_7_PENALTY=0.008825
PRIMER_RIGHT_7_PENALTY=0.057845
PRIMER_LEFT_7_SEQUENCE=AGGAGAAGACTTATTCCCTGTGG
PRIMER_RIGHT_7_SEQUENCE=AGTCATTCTTGCCACACCATACT
PRIMER_LEFT_7=1670,23
PRIMER_RIGHT_7=2178,23
PRIMER_LEFT_7_TM=60.009
PRIMER_RIGHT_7_TM=59.942
PRIMER_LEFT_7_GC_PERCENT=47.826
PRIMER_RIGHT_7_GC_PERCENT=43.478
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=3.00
PRIMER_LEFT_7_MISPRIMING_SCORE=11.00, reverse MER4A a consensus
PRIMER_RIGHT_7_MISPRIMING_SCORE=12.00, MER42a MER42 repetitive sequence: subfamily MER42a - a consensus
PRIMER_PAIR_7_MISPRIMING_SCORE=22.00, MER42a MER42 repetitive sequence: subfamily MER42a - a consensus
PRIMER_LEFT_7_END_STABILITY=8.2000
PRIMER_RIGHT_7_END_STABILITY=5.3000
PRIMER_PAIR_7_COMPL_ANY=6.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_7=509
PRIMER_PAIR_PENALTY_8=0.0706
PRIMER_LEFT_8_PENALTY=0.012719
PRIMER_RIGHT_8_PENALTY=0.057845
PRIMER_LEFT_8_SEQUENCE=TCCTGAATCACCTAACCTCAAAA
PRIMER_RIGHT_8_SEQUENCE=AGTCATTCTTGCCACACCATACT
PRIMER_LEFT_8=1992,23
PRIMER_RIGHT_8=2178,23
PRIMER_LEFT_8_TM=59.987
PRIMER_RIGHT_8_TM=59.942
PRIMER_LEFT_8_GC_PERCENT=39.130
PRIMER_RIGHT_8_GC_PERCENT=43.478
PRIMER_LEFT_8_SELF_ANY=5.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=3.00
PRIMER_LEFT_8_MISPRIMING_SCORE=12.00, LTR7 LTR from human endogenous retrovirus RTVL-H2
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, MER42a MER42 repetitive sequence: subfamily MER42a - a consensus
PRIMER_PAIR_8_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_8_END_STABILITY=7.6000
PRIMER_RIGHT_8_END_STABILITY=5.3000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_8=187
PRIMER_PAIR_PENALTY_9=0.0767
PRIMER_LEFT_9_PENALTY=0.008825
PRIMER_RIGHT_9_PENALTY=0.067837
PRIMER_LEFT_9_SEQUENCE=AGGAGAAGACTTATTCCCTGTGG
PRIMER_RIGHT_9_SEQUENCE=CCACACCATACTCTAAGCTGACC
PRIMER_LEFT_9=1670,23
PRIMER_RIGHT_9=2167,23
PRIMER_LEFT_9_TM=60.009
PRIMER_RIGHT_9_TM=60.068
PRIMER_LEFT_9_GC_PERCENT=47.826
PRIMER_RIGHT_9_GC_PERCENT=52.174
PRIMER_LEFT_9_SELF_ANY=6.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_MISPRIMING_SCORE=11.00, reverse MER4A a consensus
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_9_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_9_END_STABILITY=8.2000
PRIMER_RIGHT_9_END_STABILITY=7.9000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_9=498
PRIMER_PAIR_PENALTY_10=0.0792
PRIMER_LEFT_10_PENALTY=0.063537
PRIMER_RIGHT_10_PENALTY=0.015613
PRIMER_LEFT_10_SEQUENCE=TTGCTTCAAGCCATTAGTAAAGC
PRIMER_RIGHT_10_SEQUENCE=ATAATTTCTGAAGGCTGGACCTC
PRIMER_LEFT_10=976,23
PRIMER_RIGHT_10=1171,23
PRIMER_LEFT_10_TM=59.936
PRIMER_RIGHT_10_TM=59.984
PRIMER_LEFT_10_GC_PERCENT=39.130
PRIMER_RIGHT_10_GC_PERCENT=43.478
PRIMER_LEFT_10_SELF_ANY=7.00
PRIMER_RIGHT_10_SELF_ANY=5.00
PRIMER_LEFT_10_SELF_END=3.00
PRIMER_RIGHT_10_SELF_END=2.00
PRIMER_LEFT_10_MISPRIMING_SCORE=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_10_MISPRIMING_SCORE=12.00, MLT1b (MLT1b subfamily) - consensus sequence
PRIMER_PAIR_10_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_10_END_STABILITY=8.5000
PRIMER_RIGHT_10_END_STABILITY=7.6000
PRIMER_PAIR_10_COMPL_ANY=7.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_10=196
PRIMER_PAIR_PENALTY_11=0.0806
PRIMER_LEFT_11_PENALTY=0.012719
PRIMER_RIGHT_11_PENALTY=0.067837
PRIMER_LEFT_11_SEQUENCE=TCCTGAATCACCTAACCTCAAAA
PRIMER_RIGHT_11_SEQUENCE=CCACACCATACTCTAAGCTGACC
PRIMER_LEFT_11=1992,23
PRIMER_RIGHT_11=2167,23
PRIMER_LEFT_11_TM=59.987
PRIMER_RIGHT_11_TM=60.068
PRIMER_LEFT_11_GC_PERCENT=39.130
PRIMER_RIGHT_11_GC_PERCENT=52.174
PRIMER_LEFT_11_SELF_ANY=5.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_MISPRIMING_SCORE=12.00, LTR7 LTR from human endogenous retrovirus RTVL-H2
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_11_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_11_END_STABILITY=7.6000
PRIMER_RIGHT_11_END_STABILITY=7.9000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_11=176
PRIMER_PAIR_PENALTY_12=0.0831
PRIMER_LEFT_12_PENALTY=0.048411
PRIMER_RIGHT_12_PENALTY=0.034654
PRIMER_LEFT_12_SEQUENCE=CACAGATTTACAGTTTTGGAGCC
PRIMER_RIGHT_12_SEQUENCE=CATCATTTACGGGTAGCAGAGTC
PRIMER_LEFT_12=1750,23
PRIMER_RIGHT_12=2059,23
PRIMER_LEFT_12_TM=60.048
PRIMER_RIGHT_12_TM=60.035
PRIMER_LEFT_12_GC_PERCENT=43.478
PRIMER_RIGHT_12_GC_PERCENT=47.826
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=2.00
PRIMER_RIGHT_12_SELF_END=3.00
PRIMER_LEFT_12_MISPRIMING_SCORE=12.00, reverse MER7A Nonautonomous DNA transposon
PRIMER_RIGHT_12_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_12_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_12_END_STABILITY=9.4000
PRIMER_RIGHT_12_END_STABILITY=6.1000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_12=310
PRIMER_PAIR_PENALTY_13=0.0861
PRIMER_LEFT_13_PENALTY=0.006308
PRIMER_RIGHT_13_PENALTY=0.079770
PRIMER_LEFT_13_SEQUENCE=GAAACCTGGATATTACACCACCA
PRIMER_RIGHT_13_SEQUENCE=GTTTCACAAATCTTGTAGTCCCG
PRIMER_LEFT_13=451,23
PRIMER_RIGHT_13=791,23
PRIMER_LEFT_13_TM=59.994
PRIMER_RIGHT_13_TM=59.920
PRIMER_LEFT_13_GC_PERCENT=43.478
PRIMER_RIGHT_13_GC_PERCENT=43.478
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=6.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=2.00
PRIMER_LEFT_13_MISPRIMING_SCORE=12.00, MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_RIGHT_13_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_13_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_13_END_STABILITY=8.2000
PRIMER_RIGHT_13_END_STABILITY=11.4000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_13=341
PRIMER_PAIR_PENALTY_14=0.0954
PRIMER_LEFT_14_PENALTY=0.079770
PRIMER_RIGHT_14_PENALTY=0.015613
PRIMER_LEFT_14_SEQUENCE=CGGGACTACAAGATTTGTGAAAC
PRIMER_RIGHT_14_SEQUENCE=ATAATTTCTGAAGGCTGGACCTC
PRIMER_LEFT_14=769,23
PRIMER_RIGHT_14=1171,23
PRIMER_LEFT_14_TM=59.920
PRIMER_RIGHT_14_TM=59.984
PRIMER_LEFT_14_GC_PERCENT=43.478
PRIMER_RIGHT_14_GC_PERCENT=43.478
PRIMER_LEFT_14_SELF_ANY=6.00
PRIMER_RIGHT_14_SELF_ANY=5.00
PRIMER_LEFT_14_SELF_END=2.00
PRIMER_RIGHT_14_SELF_END=2.00
PRIMER_LEFT_14_MISPRIMING_SCORE=12.00, LTR2 LTR from human endogenous retrovirus (4-14), 3' long terminal repeat
PRIMER_RIGHT_14_MISPRIMING_SCORE=12.00, MLT1b (MLT1b subfamily) - consensus sequence
PRIMER_PAIR_14_MISPRIMING_SCORE=21.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_14_END_STABILITY=6.7000
PRIMER_RIGHT_14_END_STABILITY=7.6000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_14=403
PRIMER_PAIR_PENALTY_15=0.1032
PRIMER_LEFT_15_PENALTY=0.008825
PRIMER_RIGHT_15_PENALTY=0.094334
PRIMER_LEFT_15_SEQUENCE=AGGAGAAGACTTATTCCCTGTGG
PRIMER_RIGHT_15_SEQUENCE=TGGTTATGCTTACAGACCATCCT
PRIMER_LEFT_15=1670,23
PRIMER_RIGHT_15=2259,23
PRIMER_LEFT_15_TM=60.009
PRIMER_RIGHT_15_TM=59.906
PRIMER_LEFT_15_GC_PERCENT=47.826
PRIMER_RIGHT_15_GC_PERCENT=43.478
PRIMER_LEFT_15_SELF_ANY=6.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_MISPRIMING_SCORE=11.00, reverse MER4A a consensus
PRIMER_RIGHT_15_MISPRIMING_SCORE=12.00, L1PA11 3'-end of L1 repeat (subfamily L1PA11) - a consensus sequence
PRIMER_PAIR_15_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_15_END_STABILITY=8.2000
PRIMER_RIGHT_15_END_STABILITY=7.8000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_15=590
PRIMER_PAIR_PENALTY_16=0.1063
PRIMER_LEFT_16_PENALTY=0.048411
PRIMER_RIGHT_16_PENALTY=0.057845
PRIMER_LEFT_16_SEQUENCE=CACAGATTTACAGTTTTGGAGCC
PRIMER_RIGHT_16_SEQUENCE=AGTCATTCTTGCCACACCATACT
PRIMER_LEFT_16=1750,23
PRIMER_RIGHT_16=2178,23
PRIMER_LEFT_16_TM=60.048
PRIMER_RIGHT_16_TM=59.942
PRIMER_LEFT_16_GC_PERCENT=43.478
PRIMER_RIGHT_16_GC_PERCENT=43.478
PRIMER_LEFT_16_SELF_ANY=3.00
PRIMER_RIGHT_16_SELF_ANY=3.00
PRIMER_LEFT_16_SELF_END=2.00
PRIMER_RIGHT_16_SELF_END=3.00
PRIMER_LEFT_16_MISPRIMING_SCORE=12.00, reverse MER7A Nonautonomous DNA transposon
PRIMER_RIGHT_16_MISPRIMING_SCORE=12.00, MER42a MER42 repetitive sequence: subfamily MER42a - a consensus
PRIMER_PAIR_16_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_16_END_STABILITY=9.4000
PRIMER_RIGHT_16_END_STABILITY=5.3000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_16=429
PRIMER_PAIR_PENALTY_17=0.1071
PRIMER_LEFT_17_PENALTY=0.012719
PRIMER_RIGHT_17_PENALTY=0.094334
PRIMER_LEFT_17_SEQUENCE=TCCTGAATCACCTAACCTCAAAA
PRIMER_RIGHT_17_SEQUENCE=TGGTTATGCTTACAGACCATCCT
PRIMER_LEFT_17=1992,23
PRIMER_RIGHT_17=2259,23
PRIMER_LEFT_17_TM=59.987
PRIMER_RIGHT_17_TM=59.906
PRIMER_LEFT_17_GC_PERCENT=39.130
PRIMER_RIGHT_17_GC_PERCENT=43.478
PRIMER_LEFT_17_SELF_ANY=5.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_MISPRIMING_SCORE=12.00, LTR7 LTR from human endogenous retrovirus RTVL-H2
PRIMER_RIGHT_17_MISPRIMING_SCORE=12.00, L1PA11 3'-end of L1 repeat (subfamily L1PA11) - a consensus sequence
PRIMER_PAIR_17_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_17_END_STABILITY=7.6000
PRIMER_RIGHT_17_END_STABILITY=7.8000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_17=268
PRIMER_PAIR_PENALTY_18=0.1162
PRIMER_LEFT_18_PENALTY=0.048411
PRIMER_RIGHT_18_PENALTY=0.067837
PRIMER_LEFT_18_SEQUENCE=CACAGATTTACAGTTTTGGAGCC
PRIMER_RIGHT_18_SEQUENCE=CCACACCATACTCTAAGCTGACC
PRIMER_LEFT_18=1750,23
PRIMER_RIGHT_18=2167,23
PRIMER_LEFT_18_TM=60.048
PRIMER_RIGHT_18_TM=60.068
PRIMER_LEFT_18_GC_PERCENT=43.478
PRIMER_RIGHT_18_GC_PERCENT=52.174
PRIMER_LEFT_18_SELF_ANY=3.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=2.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_MISPRIMING_SCORE=12.00, reverse MER7A Nonautonomous DNA transposon
PRIMER_RIGHT_18_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_18_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_18_END_STABILITY=9.4000
PRIMER_RIGHT_18_END_STABILITY=7.9000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_18=418
PRIMER_PAIR_PENALTY_19=0.1215
PRIMER_LEFT_19_PENALTY=0.006308
PRIMER_RIGHT_19_PENALTY=0.115144
PRIMER_LEFT_19_SEQUENCE=GAAACCTGGATATTACACCACCA
PRIMER_RIGHT_19_SEQUENCE=AACCGGAGTAGAAATCATTGACA
PRIMER_LEFT_19=451,23
PRIMER_RIGHT_19=1222,23
PRIMER_LEFT_19_TM=59.994
PRIMER_RIGHT_19_TM=59.885
PRIMER_LEFT_19_GC_PERCENT=43.478
PRIMER_RIGHT_19_GC_PERCENT=39.130
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=5.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=3.00
PRIMER_LEFT_19_MISPRIMING_SCORE=12.00, MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_RIGHT_19_MISPRIMING_SCORE=11.00, MER4C a consensus
PRIMER_PAIR_19_MISPRIMING_SCORE=20.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_19_END_STABILITY=8.2000
PRIMER_RIGHT_19_END_STABILITY=6.7000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_19=772
=