PRIMER_SEQUENCE_ID=NM_005192
SEQUENCE=CCGGTGAGTCGCCGGCGCTGCAGAGGGAGGCGGCACTGGTCTCGACGTGGGGCGGCCAGCGATGAAGCCGCCCAGTTCAATACAAACAAGTGAGTTTGACTCATCAGATGAAGAGCCTATTGAAGATGAACAGACTCCAATTCATATATCATGGCTATCTTTGTCACGAGTGAATTGTTCTCAGTTTCTCGGTTTATGTGCTCTTCCAGGTTGTAAATTTAAAGATGTTAGAAGAAATGTCCAAAAAGATACAGAAGAACTAAAGAGCTGTGGTATACAAGACATATTTGTTTTCTGCACCAGAGGGGAACTGTCAAAATATAGAGTCCCAAACCTTCTGGATCTCTACCAGCAATGTGGAATTATCACCCATCATCATCCAATCGCAGATGGAGGGACTCCTGACATAGCCAGCTGCTGTGAAATAATGGAAGAGCTTACAACCTGCCTTAAAAATTACCGAAAAACCTTAATACACTGCTATGGAGGACTTGGGAGATCTTGTCTTGTAGCTGCTTGTCTCCTACTATACCTGTCTGACACAATATCACCAGAGCAAGCCATAGACAGCCTGCGAGACCTAAGAGGATCCGGGGCAATACAGACCATCAAGCAATACAATTATCTTCATGAGTTTCGGGACAAATTAGCTGCACATCTATCATCAAGAGATTCACAATCAAGATCTGTATCAAGATAAAGGAATTCAAATAGCATATATATGACCATGTCTGAAATGTCAGTTCTCTAGCATAATTTGTATTGAAATGAAACCACCAGTGTTATCAACTTGAATGTAAATGTACATGTGCAGATATTCCTAAAGTTTTATTGAC
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 8844, GC content failed 157, low tm 5225, high tm 1597, high any compl 36, high end compl 20, high repeat similarity 54, ok 1755
PRIMER_RIGHT_EXPLAIN=considered 8844, GC content failed 148, low tm 5855, high tm 954, high end compl 4, high repeat similarity 7, ok 1876
PRIMER_PAIR_EXPLAIN=considered 1198, unacceptable product size 1119, high end compl 4, ok 75
PRIMER_PAIR_PENALTY=0.2722
PRIMER_LEFT_PENALTY=0.123068
PRIMER_RIGHT_PENALTY=0.149159
PRIMER_LEFT_SEQUENCE=GAGGACTTGGGAGATCTTGTCTT
PRIMER_RIGHT_SEQUENCE=CCCGAAACTCATGAAGATAATTG
PRIMER_LEFT=485,23
PRIMER_RIGHT=640,23
PRIMER_LEFT_TM=60.123
PRIMER_RIGHT_TM=59.851
PRIMER_LEFT_GC_PERCENT=47.826
PRIMER_RIGHT_GC_PERCENT=39.130
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_MISPRIMING_SCORE=12.00, reverse L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_PAIR_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=7.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=156
PRIMER_PAIR_PENALTY_1=0.2911
PRIMER_LEFT_1_PENALTY=0.141958
PRIMER_RIGHT_1_PENALTY=0.149159
PRIMER_LEFT_1_SEQUENCE=AAACCTTCTGGATCTCTACCAGC
PRIMER_RIGHT_1_SEQUENCE=CCCGAAACTCATGAAGATAATTG
PRIMER_LEFT_1=330,23
PRIMER_RIGHT_1=640,23
PRIMER_LEFT_1_TM=60.142
PRIMER_RIGHT_1_TM=59.851
PRIMER_LEFT_1_GC_PERCENT=47.826
PRIMER_RIGHT_1_GC_PERCENT=39.130
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_MISPRIMING_SCORE=12.00, reverse MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, reverse L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_PAIR_1_MISPRIMING_SCORE=21.00, reverse L1MA2 3'-end of L1 repeat (subfamily L1MA2) - a consensus sequence
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=311
PRIMER_PAIR_PENALTY_2=0.2935
PRIMER_LEFT_2_PENALTY=0.144318
PRIMER_RIGHT_2_PENALTY=0.149159
PRIMER_LEFT_2_SEQUENCE=TTCTGGATCTCTACCAGCAATGT
PRIMER_RIGHT_2_SEQUENCE=CCCGAAACTCATGAAGATAATTG
PRIMER_LEFT_2=335,23
PRIMER_RIGHT_2=640,23
PRIMER_LEFT_2_TM=60.144
PRIMER_RIGHT_2_TM=59.851
PRIMER_LEFT_2_GC_PERCENT=43.478
PRIMER_RIGHT_2_GC_PERCENT=39.130
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_2_MISPRIMING_SCORE=12.00, reverse L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_PAIR_2_MISPRIMING_SCORE=23.00, reverse L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=306
PRIMER_PAIR_PENALTY_3=0.2998
PRIMER_LEFT_3_PENALTY=0.157853
PRIMER_RIGHT_3_PENALTY=0.141958
PRIMER_LEFT_3_SEQUENCE=TGAATTGTTCTCAGTTTCTCGGT
PRIMER_RIGHT_3_SEQUENCE=GCTGGTAGAGATCCAGAAGGTTT
PRIMER_LEFT_3=170,23
PRIMER_RIGHT_3=352,23
PRIMER_LEFT_3_TM=60.158
PRIMER_RIGHT_3_TM=60.142
PRIMER_LEFT_3_GC_PERCENT=39.130
PRIMER_RIGHT_3_GC_PERCENT=47.826
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, reverse MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, Tigger1 Autonomous DNA transposon
PRIMER_PAIR_3_MISPRIMING_SCORE=22.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_3_END_STABILITY=9.6000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=183
PRIMER_PAIR_PENALTY_4=0.2998
PRIMER_LEFT_4_PENALTY=0.157853
PRIMER_RIGHT_4_PENALTY=0.141958
PRIMER_LEFT_4_SEQUENCE=GTGAATTGTTCTCAGTTTCTCGG
PRIMER_RIGHT_4_SEQUENCE=GCTGGTAGAGATCCAGAAGGTTT
PRIMER_LEFT_4=169,23
PRIMER_RIGHT_4=352,23
PRIMER_LEFT_4_TM=60.158
PRIMER_RIGHT_4_TM=60.142
PRIMER_LEFT_4_GC_PERCENT=43.478
PRIMER_RIGHT_4_GC_PERCENT=47.826
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, reverse MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, Tigger1 Autonomous DNA transposon
PRIMER_PAIR_4_MISPRIMING_SCORE=23.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_4_END_STABILITY=9.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=184
PRIMER_PAIR_PENALTY_5=0.3700
PRIMER_LEFT_5_PENALTY=0.141958
PRIMER_RIGHT_5_PENALTY=0.228032
PRIMER_LEFT_5_SEQUENCE=AAACCTTCTGGATCTCTACCAGC
PRIMER_RIGHT_5_SEQUENCE=TTCGGTAATTTTTAAGGCAGGTT
PRIMER_LEFT_5=330,23
PRIMER_RIGHT_5=463,23
PRIMER_LEFT_5_TM=60.142
PRIMER_RIGHT_5_TM=60.228
PRIMER_LEFT_5_GC_PERCENT=47.826
PRIMER_RIGHT_5_GC_PERCENT=34.783
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=2.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_MISPRIMING_SCORE=12.00, reverse MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_5_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_5_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_5_END_STABILITY=9.7000
PRIMER_RIGHT_5_END_STABILITY=7.9000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_5=134
PRIMER_PAIR_PENALTY_6=0.3724
PRIMER_LEFT_6_PENALTY=0.144318
PRIMER_RIGHT_6_PENALTY=0.228032
PRIMER_LEFT_6_SEQUENCE=TTCTGGATCTCTACCAGCAATGT
PRIMER_RIGHT_6_SEQUENCE=TTCGGTAATTTTTAAGGCAGGTT
PRIMER_LEFT_6=335,23
PRIMER_RIGHT_6=463,23
PRIMER_LEFT_6_TM=60.144
PRIMER_RIGHT_6_TM=60.228
PRIMER_LEFT_6_GC_PERCENT=43.478
PRIMER_RIGHT_6_GC_PERCENT=34.783
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_6_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_6_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_6_END_STABILITY=6.6000
PRIMER_RIGHT_6_END_STABILITY=7.9000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_6=129
PRIMER_PAIR_PENALTY_7=0.3772
PRIMER_LEFT_7_PENALTY=0.228032
PRIMER_RIGHT_7_PENALTY=0.149159
PRIMER_LEFT_7_SEQUENCE=AACCTGCCTTAAAAATTACCGAA
PRIMER_RIGHT_7_SEQUENCE=CCCGAAACTCATGAAGATAATTG
PRIMER_LEFT_7=441,23
PRIMER_RIGHT_7=640,23
PRIMER_LEFT_7_TM=60.228
PRIMER_RIGHT_7_TM=59.851
PRIMER_LEFT_7_GC_PERCENT=34.783
PRIMER_RIGHT_7_GC_PERCENT=39.130
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=2.00
PRIMER_LEFT_7_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_7_MISPRIMING_SCORE=12.00, reverse L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_PAIR_7_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_7_END_STABILITY=10.2000
PRIMER_RIGHT_7_END_STABILITY=7.2000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_7=200
PRIMER_PAIR_PENALTY_8=0.3859
PRIMER_LEFT_8_PENALTY=0.157853
PRIMER_RIGHT_8_PENALTY=0.228032
PRIMER_LEFT_8_SEQUENCE=TGAATTGTTCTCAGTTTCTCGGT
PRIMER_RIGHT_8_SEQUENCE=TTCGGTAATTTTTAAGGCAGGTT
PRIMER_LEFT_8=170,23
PRIMER_RIGHT_8=463,23
PRIMER_LEFT_8_TM=60.158
PRIMER_RIGHT_8_TM=60.228
PRIMER_LEFT_8_GC_PERCENT=39.130
PRIMER_RIGHT_8_GC_PERCENT=34.783
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_MISPRIMING_SCORE=12.00, reverse MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_8_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_8_MISPRIMING_SCORE=20.00, reverse MER34 a consensus
PRIMER_LEFT_8_END_STABILITY=9.6000
PRIMER_RIGHT_8_END_STABILITY=7.9000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_8=294
PRIMER_PAIR_PENALTY_9=0.3859
PRIMER_LEFT_9_PENALTY=0.157853
PRIMER_RIGHT_9_PENALTY=0.228032
PRIMER_LEFT_9_SEQUENCE=GTGAATTGTTCTCAGTTTCTCGG
PRIMER_RIGHT_9_SEQUENCE=TTCGGTAATTTTTAAGGCAGGTT
PRIMER_LEFT_9=169,23
PRIMER_RIGHT_9=463,23
PRIMER_LEFT_9_TM=60.158
PRIMER_RIGHT_9_TM=60.228
PRIMER_LEFT_9_GC_PERCENT=43.478
PRIMER_RIGHT_9_GC_PERCENT=34.783
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_MISPRIMING_SCORE=12.00, reverse MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_9_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_9_MISPRIMING_SCORE=21.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_9_END_STABILITY=9.9000
PRIMER_RIGHT_9_END_STABILITY=7.9000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_9=295
PRIMER_PAIR_PENALTY_10=0.4120
PRIMER_LEFT_10_PENALTY=0.141958
PRIMER_RIGHT_10_PENALTY=0.270039
PRIMER_LEFT_10_SEQUENCE=AAACCTTCTGGATCTCTACCAGC
PRIMER_RIGHT_10_SEQUENCE=AGCTCTTCCATTATTTCACAGCA
PRIMER_LEFT_10=330,23
PRIMER_RIGHT_10=437,23
PRIMER_LEFT_10_TM=60.142
PRIMER_RIGHT_10_TM=60.270
PRIMER_LEFT_10_GC_PERCENT=47.826
PRIMER_RIGHT_10_GC_PERCENT=39.130
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=2.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_MISPRIMING_SCORE=12.00, reverse MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_10_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_10_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_10_END_STABILITY=9.7000
PRIMER_RIGHT_10_END_STABILITY=8.5000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_10=108
PRIMER_PAIR_PENALTY_11=0.4120
PRIMER_LEFT_11_PENALTY=0.141958
PRIMER_RIGHT_11_PENALTY=0.270039
PRIMER_LEFT_11_SEQUENCE=AAACCTTCTGGATCTCTACCAGC
PRIMER_RIGHT_11_SEQUENCE=GCTCTTCCATTATTTCACAGCAG
PRIMER_LEFT_11=330,23
PRIMER_RIGHT_11=436,23
PRIMER_LEFT_11_TM=60.142
PRIMER_RIGHT_11_TM=60.270
PRIMER_LEFT_11_GC_PERCENT=47.826
PRIMER_RIGHT_11_GC_PERCENT=43.478
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=2.00
PRIMER_RIGHT_11_SELF_END=2.00
PRIMER_LEFT_11_MISPRIMING_SCORE=12.00, reverse MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_11_MISPRIMING_SCORE=23.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_11_END_STABILITY=9.7000
PRIMER_RIGHT_11_END_STABILITY=8.2000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_11=107
PRIMER_PAIR_PENALTY_12=0.4120
PRIMER_LEFT_12_PENALTY=0.141958
PRIMER_RIGHT_12_PENALTY=0.270039
PRIMER_LEFT_12_SEQUENCE=AAACCTTCTGGATCTCTACCAGC
PRIMER_RIGHT_12_SEQUENCE=CTCTTCCATTATTTCACAGCAGC
PRIMER_LEFT_12=330,23
PRIMER_RIGHT_12=435,23
PRIMER_LEFT_12_TM=60.142
PRIMER_RIGHT_12_TM=60.270
PRIMER_LEFT_12_GC_PERCENT=47.826
PRIMER_RIGHT_12_GC_PERCENT=43.478
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=2.00
PRIMER_RIGHT_12_SELF_END=3.00
PRIMER_LEFT_12_MISPRIMING_SCORE=12.00, reverse MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_12_MISPRIMING_SCORE=12.00, MER4C a consensus
PRIMER_PAIR_12_MISPRIMING_SCORE=23.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_12_END_STABILITY=9.7000
PRIMER_RIGHT_12_END_STABILITY=9.7000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_12=106
PRIMER_PAIR_PENALTY_13=0.4144
PRIMER_LEFT_13_PENALTY=0.144318
PRIMER_RIGHT_13_PENALTY=0.270039
PRIMER_LEFT_13_SEQUENCE=TTCTGGATCTCTACCAGCAATGT
PRIMER_RIGHT_13_SEQUENCE=AGCTCTTCCATTATTTCACAGCA
PRIMER_LEFT_13=335,23
PRIMER_RIGHT_13=437,23
PRIMER_LEFT_13_TM=60.144
PRIMER_RIGHT_13_TM=60.270
PRIMER_LEFT_13_GC_PERCENT=43.478
PRIMER_RIGHT_13_GC_PERCENT=39.130
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_13_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_13_MISPRIMING_SCORE=24.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_13_END_STABILITY=6.6000
PRIMER_RIGHT_13_END_STABILITY=8.5000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_13=103
PRIMER_PAIR_PENALTY_14=0.4144
PRIMER_LEFT_14_PENALTY=0.144318
PRIMER_RIGHT_14_PENALTY=0.270039
PRIMER_LEFT_14_SEQUENCE=TTCTGGATCTCTACCAGCAATGT
PRIMER_RIGHT_14_SEQUENCE=GCTCTTCCATTATTTCACAGCAG
PRIMER_LEFT_14=335,23
PRIMER_RIGHT_14=436,23
PRIMER_LEFT_14_TM=60.144
PRIMER_RIGHT_14_TM=60.270
PRIMER_LEFT_14_GC_PERCENT=43.478
PRIMER_RIGHT_14_GC_PERCENT=43.478
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=2.00
PRIMER_LEFT_14_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_14_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_14_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_14_END_STABILITY=6.6000
PRIMER_RIGHT_14_END_STABILITY=8.2000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_14=102
PRIMER_PAIR_PENALTY_15=0.4144
PRIMER_LEFT_15_PENALTY=0.144318
PRIMER_RIGHT_15_PENALTY=0.270039
PRIMER_LEFT_15_SEQUENCE=TTCTGGATCTCTACCAGCAATGT
PRIMER_RIGHT_15_SEQUENCE=CTCTTCCATTATTTCACAGCAGC
PRIMER_LEFT_15=335,23
PRIMER_RIGHT_15=435,23
PRIMER_LEFT_15_TM=60.144
PRIMER_RIGHT_15_TM=60.270
PRIMER_LEFT_15_GC_PERCENT=43.478
PRIMER_RIGHT_15_GC_PERCENT=43.478
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=3.00
PRIMER_LEFT_15_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_15_MISPRIMING_SCORE=12.00, MER4C a consensus
PRIMER_PAIR_15_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_15_END_STABILITY=6.6000
PRIMER_RIGHT_15_END_STABILITY=9.7000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_15=101
PRIMER_PAIR_PENALTY_16=0.4192
PRIMER_LEFT_16_PENALTY=0.270039
PRIMER_RIGHT_16_PENALTY=0.149159
PRIMER_LEFT_16_SEQUENCE=AGCTGCTGTGAAATAATGGAAGA
PRIMER_RIGHT_16_SEQUENCE=CCCGAAACTCATGAAGATAATTG
PRIMER_LEFT_16=412,23
PRIMER_RIGHT_16=640,23
PRIMER_LEFT_16_TM=60.270
PRIMER_RIGHT_16_TM=59.851
PRIMER_LEFT_16_GC_PERCENT=39.130
PRIMER_RIGHT_16_GC_PERCENT=39.130
PRIMER_LEFT_16_SELF_ANY=5.00
PRIMER_RIGHT_16_SELF_ANY=6.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=2.00
PRIMER_LEFT_16_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_16_MISPRIMING_SCORE=12.00, reverse L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_PAIR_16_MISPRIMING_SCORE=20.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_16_END_STABILITY=6.7000
PRIMER_RIGHT_16_END_STABILITY=7.2000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_16=229
PRIMER_PAIR_PENALTY_17=0.4192
PRIMER_LEFT_17_PENALTY=0.270039
PRIMER_RIGHT_17_PENALTY=0.149159
PRIMER_LEFT_17_SEQUENCE=GCTGCTGTGAAATAATGGAAGAG
PRIMER_RIGHT_17_SEQUENCE=CCCGAAACTCATGAAGATAATTG
PRIMER_LEFT_17=413,23
PRIMER_RIGHT_17=640,23
PRIMER_LEFT_17_TM=60.270
PRIMER_RIGHT_17_TM=59.851
PRIMER_LEFT_17_GC_PERCENT=43.478
PRIMER_RIGHT_17_GC_PERCENT=39.130
PRIMER_LEFT_17_SELF_ANY=3.00
PRIMER_RIGHT_17_SELF_ANY=6.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=2.00
PRIMER_LEFT_17_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_17_MISPRIMING_SCORE=12.00, reverse L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_PAIR_17_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_17_END_STABILITY=6.7000
PRIMER_RIGHT_17_END_STABILITY=7.2000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_17=228
PRIMER_PAIR_PENALTY_18=0.4279
PRIMER_LEFT_18_PENALTY=0.157853
PRIMER_RIGHT_18_PENALTY=0.270039
PRIMER_LEFT_18_SEQUENCE=TGAATTGTTCTCAGTTTCTCGGT
PRIMER_RIGHT_18_SEQUENCE=AGCTCTTCCATTATTTCACAGCA
PRIMER_LEFT_18=170,23
PRIMER_RIGHT_18=437,23
PRIMER_LEFT_18_TM=60.158
PRIMER_RIGHT_18_TM=60.270
PRIMER_LEFT_18_GC_PERCENT=39.130
PRIMER_RIGHT_18_GC_PERCENT=39.130
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_MISPRIMING_SCORE=12.00, reverse MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_18_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_18_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_18_END_STABILITY=9.6000
PRIMER_RIGHT_18_END_STABILITY=8.5000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_18=268
PRIMER_PAIR_PENALTY_19=0.4279
PRIMER_LEFT_19_PENALTY=0.157853
PRIMER_RIGHT_19_PENALTY=0.270039
PRIMER_LEFT_19_SEQUENCE=TGAATTGTTCTCAGTTTCTCGGT
PRIMER_RIGHT_19_SEQUENCE=CTCTTCCATTATTTCACAGCAGC
PRIMER_LEFT_19=170,23
PRIMER_RIGHT_19=435,23
PRIMER_LEFT_19_TM=60.158
PRIMER_RIGHT_19_TM=60.270
PRIMER_LEFT_19_GC_PERCENT=39.130
PRIMER_RIGHT_19_GC_PERCENT=43.478
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=3.00
PRIMER_LEFT_19_MISPRIMING_SCORE=12.00, reverse MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_19_MISPRIMING_SCORE=12.00, MER4C a consensus
PRIMER_PAIR_19_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_19_END_STABILITY=9.6000
PRIMER_RIGHT_19_END_STABILITY=9.7000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_19=266
=