PRIMER_SEQUENCE_ID=NM_005401
SEQUENCE=CCTTCGCCCCTCCTCCGCCAGTCACTGGAATCAATTCCGTGGGGAATCGGCTCCGCCGCCGCGAAGGACAGCCTTTCCGCGCGGGACTCCGGGGCGCCACGGGGGCCATGTAAGCAGCTATCTTCCAGAGGGCCACACTGGGCATGGACACCCTTTTCCCTGCCTGGAGGAGCACAGGTGATAGTGTAATTTTCCAGTCACGAAACTGCTAAGGCCATCTCAGGGGCGTGTGCGCCAGGATAGGCAGGCGGCGTCCGAGGACCACATAGCCATGCCTTTTGGTCTGAAGCTCCGCCGGACACGGCGCTACAACGTCCTGAGCAAGAACTGCTTTGTCACACGGATTCGCCTGCTGGACAGCAATGTTATCGAGTGCACGCTGTCGGTGGAAAGCACAGGGCAAGAATGCCTGGAGGCTGTGGCCCAGAGGCTGGAGCTGCGAGAGACGCACTACTTTGGCCTTTGGTTTCTCAGCAAGAGCCAGCAAGCACGATGGGTGGAGCTGGAGAAACCTCTGAAGAAACATCTGGACAAATTCGCTAATGAGCCTTTGCTTTTCTTTGGAGTCATGTTCTATGTGCCAAATGTGTCATGGCTTCAGCAAGAGGCCACAAGATATCAGTATTACCTGCAAGTCAAAAAAGATGTGCTTGAAGGGCGATTACGATGTACATTGGACCAGGTGATTCGGCTAGCCGGCCTAGCTGTGCAAGCTGATTTTGGAGACTATAATCAGTTTGATTCTCAAGATTTCCTCAGAGAGTATGTGCTATTTCCTATGGATTTGGCCCTGGAAGAGGCTGTTCTGGAGGAGCTGACCCAGAAGGTAGCCCAAGAACACAAAGCCCACAGTGGAATCCTGCCAGCAGAAGCTGAACTGATGTACATCAATGAAGTTGAACGTTTGGATGGATTTGGACAGGAAATCTTCCCTGTAAAGGACAATCATGGAAACTGTGTACACCTTGGCATTTTCTTTATGGGGATTTTCGTGAGGAACAGAATTGGAAGACAAGCGGTAATATACAGGTGGAATGACATGGGGAATATCACTCATAACAAGTCGACCATTCTAGTGGAGCTCATCAACAAAGAAGAGACTGCCCTCTTTCACACGGATGATATCGAAAATGCCAAGTATATTTCTCGGTTGTTTGCCACACGACACAAGTTTTACAAACAAAACAAAATCTGCACTGAACAGTCAAATTCTCCACCCCCCATCAGACGCCAGCCCACCTGGAGCCGATCCTCTCTGCCCAGGCAGCAGCCGTACATCCTGCCTCCCGTTCACGTCCAGTGTGGTGAGCACTACTCGGAAACGCACACCTCGCAAGACAGCATTTTTCATGGGAATGAAGAAGCCTTGTATTGCAACTCTCACAACAGCCTGGACTTAAATTATTTAAATGGCACTGTCACCAATGGCAGCGTGTGTAGCGTTCACAGCGTCAACTCCCTCAACTGCTCGCAAAGTTTCATCCAGGCCTCCCCTGTATCCTCCAACCTCAGTATCCCTGGGAGTGACATCATGCGGGCCGACTACATCCCGAGCCACCGGCACAGCGCGATCATCGTGCCCTCGTACAGGCCAACCCCCGATTATGAGACAGTCATGCGCCAGATGAAGAGGGGGATCCTGCATACAGACAGCCAGAGCCAGTCTCTGAGAAACCTCAACATTATCAACACCCATGCCTACAACCAGCCAGAGGATCTGGTGTACAGCCAACCGGAGATGCGGGAGAGGCACCCCTACACTGTCCCTTATGGGCCACAGGGGGTCTACAGCAACAAACTTGTCAGTCCATCTGACCAGAGGAACCCAAAGAATAATGTGGTACCAAGCAAGCCGGGGGCAAGCGCCATCTCGCACACGGTGAGCACCCCAGAGCTGGCCAACATGCAGCTGCAGGGCAGCCATAACTACAGCACGGCCCACATGCTTAAGAACTATCTCTTCAGGCCACCGCCCCCCTACCCACGGCCACGACCTGCCACCAGCACCCCAGACCTGGCCAGCCACCGCCACAAGTACGTCAGCGGCAGCAGCCCGGACCTGGTGACCCGGAAGGTGCAGCTCTCGGTGAAGACCTTCCAAGAGGACAGCTCTCCGGTGGTTCATCAGTCTCTCCAGGAGGTGAGCGAGCCCCTCACGGCCACCAAGCACCACGGCACTGTGAACAAGCGCCACAGCCTGGAGGTGATGAACAGCATGGTGCGGGGCATGGAGGCCATGACGCTCAAGTCGCTCCACCTCCCCATGGCTCGCCGCAACACGCTCCGGGAGCAGGGACCGCCCGAGGAGGGGTCAGGCAGCCACGAGGTCCCCCAGCTCCCTCAGTATCACCACAAGAAGACCTTCTCTGATGCCACTATGCTAATCCACAGCAGCGAGAGTGAGGAGGAGGAGGAGGAGGCTCCAGAATCGGTGCCCCAGATCCCCATGCTCCGGGAGAAGATGGAGTACAGTGCCCAGCTGCAGGCGGCCCTGGCCCGCATCCCCAACAAGCCCCCGCCTGAGTACCCCGGTCCAAGGAAGAGTGTGAGCAATGGGGCTCTGAGGCAGGACCAAGCCAGCCTTCCTCCCGCCATGGCCAGAGCCAGGGTGCTGAGGCATGGGCCAGCCAAGGCCATCAGCATGTCTCGGACTGACCCGCCGGCTGTCAACGGGGCCTCTCTCGGCCCATCCATCTCGGAACCCGACCTGACTAGTGTGAAGGAGCGGGTCAAAAAAGAGCCTGTGAAGGAGAGACCTGTGTCTGAAATGTTTTCCCTGGAAGACAGCATTATAGAGAGAGAGATGATGATCAGGAATCTAGAGAAGCAGAAGATGGCAGGCCTGGAGGCACAGAAGAGGCCGCTGATGTTGGCAGCATTGAATGGGCTCTCGGTGGCTCGAGTCTCAGGGCGGGAAGAGAATCGAGTTGATGCCACCCGGGTTCCCATGGACGAGAGGTTCAGAACCCTGAAGAAGAAACTAGAAGAGGGAATGGTGTTCACAGAATATGAGCAAATTCCAAAGAAAAAGGCGAATGGCATTTTCAGCACAGCAGCTCTGCCAGAAAACGCCGAGCGCAGCCGAATCCGTGAAGTTGTCCCCTATGAGGAGAATCGAGTAGAGCTGATACCAACCAAAGAAAATAACACAGGATACATTAATGCCTCCCACATCAAGGTGGTGGTTGGCGGGGCAGAATGGCACTACATAGCCACCCAGGGGCCCCTGCCACACACGTGCCACGACTTCTGGCAGATGGTGTGGGAGCAGGGAGTGAATGTGATTGCCATGGTCACTGCAGAGGAGGAGGGTGGACGAACCAAAAGCCACCGATACTGGCCCAAACTAGGTTCAAAGCACAGCTCAGCCACCTATGGCAAGTTCAAGGTCACCACGAAGTTTCGAACGGATTCTGTTTGCTATGCAACCACGGGCTTGAAGGTCAAGCACCTTTTGTCTGGGCAAGAAAGGACGGTGTGGCATTTACAATATACTGACTGGCCAGATCACGGCTGTCCAGAAGATGTCCAAGGATTTTTATCCTACTTGGAGGAGATCCAGTCGGTCCGTCGCCATACCAACAGCATGCTGGAAGGCACCAAGAACCGGCACCCGCCCATCGTGGTCCACTGTAGTGCTGGGGTGGGAAGGACCGGCGTGCTCATTCTTTCTGAGCTGATGATCTACTGCTTGGAACATAACGAAAAGGTGGAAGTGCCCATGATGCTGAGGCTCCTCAGGGAGCAGAGGATGTTCATGATCCAGACTATCGCTCAGTACAAGTTTGTCTACCAAGTCCTCATCCAGTTCCTCCAAAACTCCAGACTCATTTAATCACCCCAATCCAGCTCCTGGAGGAGGGACCCAGCTCCATCGCGCTGGAGGAGAGTCACCTCCAGACAACATCTGCTCCCCCCACAGGGGTGCAGGTGGCTGGCAGCAAACAGGCTCTCTGAAGACAGTAGCCAAGATTATTCACACATACCATGTATTATTTTATATGAGATAATTTATTTTTTTCCCCTTTGGAATAACTTTTGTGAATTATTATAATGCAGTTTCCCTAGTAATATAGTACTTTTCATTTG
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 47075, GC content failed 713, low tm 14916, high tm 21598, high any compl 2, high end compl 42, high repeat similarity 45, long poly-x seq 104, ok 9655
PRIMER_RIGHT_EXPLAIN=considered 46935, GC content failed 891, low tm 15292, high tm 20954, high any compl 1, high end compl 6, high repeat similarity 12, long poly-x seq 122, ok 9657
PRIMER_PAIR_EXPLAIN=considered 869, unacceptable product size 796, high end compl 11, ok 62
PRIMER_PAIR_PENALTY=0.0218
PRIMER_LEFT_PENALTY=0.006308
PRIMER_RIGHT_PENALTY=0.015502
PRIMER_LEFT_SEQUENCE=GATTTTCGTGAGGAACAGAATTG
PRIMER_RIGHT_SEQUENCE=TTTGTAAAACTTGTGTCGTGTGG
PRIMER_LEFT=984,23
PRIMER_RIGHT=1179,23
PRIMER_LEFT_TM=59.994
PRIMER_RIGHT_TM=59.984
PRIMER_LEFT_GC_PERCENT=39.130
PRIMER_RIGHT_GC_PERCENT=39.130
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_MISPRIMING_SCORE=11.00, MER34 a consensus
PRIMER_PAIR_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=196
PRIMER_PAIR_PENALTY_1=0.0297
PRIMER_LEFT_1_PENALTY=0.014220
PRIMER_RIGHT_1_PENALTY=0.015502
PRIMER_LEFT_1_SEQUENCE=ATTACGATGTACATTGGACCAGG
PRIMER_RIGHT_1_SEQUENCE=TTTGTAAAACTTGTGTCGTGTGG
PRIMER_LEFT_1=660,23
PRIMER_RIGHT_1=1179,23
PRIMER_LEFT_1_TM=60.014
PRIMER_RIGHT_1_TM=59.984
PRIMER_LEFT_1_GC_PERCENT=43.478
PRIMER_RIGHT_1_GC_PERCENT=39.130
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_MISPRIMING_SCORE=12.00, MLT1f (MLT1f subfamily) - consensus sequence
PRIMER_RIGHT_1_MISPRIMING_SCORE=11.00, MER34 a consensus
PRIMER_PAIR_1_MISPRIMING_SCORE=21.00, MER34 a consensus
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=520
PRIMER_PAIR_PENALTY_2=0.0547
PRIMER_LEFT_2_PENALTY=0.006308
PRIMER_RIGHT_2_PENALTY=0.048411
PRIMER_LEFT_2_SEQUENCE=GATTTTCGTGAGGAACAGAATTG
PRIMER_RIGHT_2_SEQUENCE=AATTTAAGTCCAGGCTGTTGTGA
PRIMER_LEFT_2=984,23
PRIMER_RIGHT_2=1402,23
PRIMER_LEFT_2_TM=59.994
PRIMER_RIGHT_2_TM=60.048
PRIMER_LEFT_2_GC_PERCENT=39.130
PRIMER_RIGHT_2_GC_PERCENT=39.130
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_2_MISPRIMING_SCORE=12.00, CER Human D22Z3 repetitive DNA (centromeric DNA)
PRIMER_PAIR_2_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=419
PRIMER_PAIR_PENALTY_3=0.0612
PRIMER_LEFT_3_PENALTY=0.014220
PRIMER_RIGHT_3_PENALTY=0.046971
PRIMER_LEFT_3_SEQUENCE=ATTACGATGTACATTGGACCAGG
PRIMER_RIGHT_3_SEQUENCE=TCCACTAGAATGGTCGACTTGTT
PRIMER_LEFT_3=660,23
PRIMER_RIGHT_3=1079,23
PRIMER_LEFT_3_TM=60.014
PRIMER_RIGHT_3_TM=60.047
PRIMER_LEFT_3_GC_PERCENT=43.478
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, MLT1f (MLT1f subfamily) - consensus sequence
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_3_MISPRIMING_SCORE=24.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=420
PRIMER_PAIR_PENALTY_4=0.0625
PRIMER_LEFT_4_PENALTY=0.046971
PRIMER_RIGHT_4_PENALTY=0.015502
PRIMER_LEFT_4_SEQUENCE=AACAAGTCGACCATTCTAGTGGA
PRIMER_RIGHT_4_SEQUENCE=TTTGTAAAACTTGTGTCGTGTGG
PRIMER_LEFT_4=1057,23
PRIMER_RIGHT_4=1179,23
PRIMER_LEFT_4_TM=60.047
PRIMER_RIGHT_4_TM=59.984
PRIMER_LEFT_4_GC_PERCENT=43.478
PRIMER_RIGHT_4_GC_PERCENT=39.130
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, reverse MIR2 Repetitive element - a consensus
PRIMER_RIGHT_4_MISPRIMING_SCORE=11.00, MER34 a consensus
PRIMER_PAIR_4_MISPRIMING_SCORE=22.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=123
PRIMER_PAIR_PENALTY_5=0.0626
PRIMER_LEFT_5_PENALTY=0.014220
PRIMER_RIGHT_5_PENALTY=0.048411
PRIMER_LEFT_5_SEQUENCE=ATTACGATGTACATTGGACCAGG
PRIMER_RIGHT_5_SEQUENCE=AATTTAAGTCCAGGCTGTTGTGA
PRIMER_LEFT_5=660,23
PRIMER_RIGHT_5=1402,23
PRIMER_LEFT_5_TM=60.014
PRIMER_RIGHT_5_TM=60.048
PRIMER_LEFT_5_GC_PERCENT=43.478
PRIMER_RIGHT_5_GC_PERCENT=39.130
PRIMER_LEFT_5_SELF_ANY=8.00
PRIMER_RIGHT_5_SELF_ANY=5.00
PRIMER_LEFT_5_SELF_END=3.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_MISPRIMING_SCORE=12.00, MLT1f (MLT1f subfamily) - consensus sequence
PRIMER_RIGHT_5_MISPRIMING_SCORE=12.00, CER Human D22Z3 repetitive DNA (centromeric DNA)
PRIMER_PAIR_5_MISPRIMING_SCORE=20.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_5_END_STABILITY=9.7000
PRIMER_RIGHT_5_END_STABILITY=6.7000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_5=743
PRIMER_PAIR_PENALTY_6=0.0706
PRIMER_LEFT_6_PENALTY=0.014220
PRIMER_RIGHT_6_PENALTY=0.056374
PRIMER_LEFT_6_SEQUENCE=ATTACGATGTACATTGGACCAGG
PRIMER_RIGHT_6_SEQUENCE=CAAGGTGTACACAGTTTCCATGA
PRIMER_LEFT_6=660,23
PRIMER_RIGHT_6=967,23
PRIMER_LEFT_6_TM=60.014
PRIMER_RIGHT_6_TM=59.944
PRIMER_LEFT_6_GC_PERCENT=43.478
PRIMER_RIGHT_6_GC_PERCENT=43.478
PRIMER_LEFT_6_SELF_ANY=8.00
PRIMER_RIGHT_6_SELF_ANY=8.00
PRIMER_LEFT_6_SELF_END=3.00
PRIMER_RIGHT_6_SELF_END=2.00
PRIMER_LEFT_6_MISPRIMING_SCORE=12.00, MLT1f (MLT1f subfamily) - consensus sequence
PRIMER_RIGHT_6_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_6_MISPRIMING_SCORE=22.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_6_END_STABILITY=9.7000
PRIMER_RIGHT_6_END_STABILITY=6.9000
PRIMER_PAIR_6_COMPL_ANY=6.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_6=308
PRIMER_PAIR_PENALTY_7=0.0888
PRIMER_LEFT_7_PENALTY=0.006308
PRIMER_RIGHT_7_PENALTY=0.082508
PRIMER_LEFT_7_SEQUENCE=GATTTTCGTGAGGAACAGAATTG
PRIMER_RIGHT_7_SEQUENCE=GCTTGCTTGGTACCACATTATTC
PRIMER_LEFT_7=984,23
PRIMER_RIGHT_7=1852,23
PRIMER_LEFT_7_TM=59.994
PRIMER_RIGHT_7_TM=59.917
PRIMER_LEFT_7_GC_PERCENT=39.130
PRIMER_RIGHT_7_GC_PERCENT=43.478
PRIMER_LEFT_7_SELF_ANY=5.00
PRIMER_RIGHT_7_SELF_ANY=8.00
PRIMER_LEFT_7_SELF_END=2.00
PRIMER_RIGHT_7_SELF_END=2.00
PRIMER_LEFT_7_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_7_MISPRIMING_SCORE=11.00, L1PA11 3'-end of L1 repeat (subfamily L1PA11) - a consensus sequence
PRIMER_PAIR_7_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_7_END_STABILITY=7.2000
PRIMER_RIGHT_7_END_STABILITY=5.9000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_7=869
PRIMER_PAIR_PENALTY_8=0.0948
PRIMER_LEFT_8_PENALTY=0.006308
PRIMER_RIGHT_8_PENALTY=0.088469
PRIMER_LEFT_8_SEQUENCE=GATTTTCGTGAGGAACAGAATTG
PRIMER_RIGHT_8_SEQUENCE=ACAACCGAGAAATATACTTGGCA
PRIMER_LEFT_8=984,23
PRIMER_RIGHT_8=1153,23
PRIMER_LEFT_8_TM=59.994
PRIMER_RIGHT_8_TM=59.912
PRIMER_LEFT_8_GC_PERCENT=39.130
PRIMER_RIGHT_8_GC_PERCENT=39.130
PRIMER_LEFT_8_SELF_ANY=5.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=2.00
PRIMER_RIGHT_8_SELF_END=3.00
PRIMER_LEFT_8_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_8_MISPRIMING_SCORE=24.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_8_END_STABILITY=7.2000
PRIMER_RIGHT_8_END_STABILITY=10.0000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_8=170
PRIMER_PAIR_PENALTY_9=0.0950
PRIMER_LEFT_9_PENALTY=0.006308
PRIMER_RIGHT_9_PENALTY=0.088682
PRIMER_LEFT_9_SEQUENCE=GATTTTCGTGAGGAACAGAATTG
PRIMER_RIGHT_9_SEQUENCE=GGGATACTGAGGTTGGAGGATAC
PRIMER_LEFT_9=984,23
PRIMER_RIGHT_9=1517,23
PRIMER_LEFT_9_TM=59.994
PRIMER_RIGHT_9_TM=60.089
PRIMER_LEFT_9_GC_PERCENT=39.130
PRIMER_RIGHT_9_GC_PERCENT=52.174
PRIMER_LEFT_9_SELF_ANY=5.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=2.00
PRIMER_RIGHT_9_SELF_END=2.00
PRIMER_LEFT_9_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, MLT1f (MLT1f subfamily) - consensus sequence
PRIMER_PAIR_9_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_9_END_STABILITY=7.2000
PRIMER_RIGHT_9_END_STABILITY=5.3000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_9=534
PRIMER_PAIR_PENALTY_10=0.0954
PRIMER_LEFT_10_PENALTY=0.046971
PRIMER_RIGHT_10_PENALTY=0.048411
PRIMER_LEFT_10_SEQUENCE=AACAAGTCGACCATTCTAGTGGA
PRIMER_RIGHT_10_SEQUENCE=AATTTAAGTCCAGGCTGTTGTGA
PRIMER_LEFT_10=1057,23
PRIMER_RIGHT_10=1402,23
PRIMER_LEFT_10_TM=60.047
PRIMER_RIGHT_10_TM=60.048
PRIMER_LEFT_10_GC_PERCENT=43.478
PRIMER_RIGHT_10_GC_PERCENT=39.130
PRIMER_LEFT_10_SELF_ANY=6.00
PRIMER_RIGHT_10_SELF_ANY=5.00
PRIMER_LEFT_10_SELF_END=3.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_MISPRIMING_SCORE=12.00, reverse MIR2 Repetitive element - a consensus
PRIMER_RIGHT_10_MISPRIMING_SCORE=12.00, CER Human D22Z3 repetitive DNA (centromeric DNA)
PRIMER_PAIR_10_MISPRIMING_SCORE=21.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_10_END_STABILITY=7.9000
PRIMER_RIGHT_10_END_STABILITY=6.7000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_10=346
PRIMER_PAIR_PENALTY_11=0.0987
PRIMER_LEFT_11_PENALTY=0.048411
PRIMER_RIGHT_11_PENALTY=0.050278
PRIMER_LEFT_11_SEQUENCE=TGTAAGCAGCTATCTTCCAGAGG
PRIMER_RIGHT_11_SEQUENCE=GAGAAACCAAAGGCCAAAGTAGT
PRIMER_LEFT_11=108,23
PRIMER_RIGHT_11=471,23
PRIMER_LEFT_11_TM=60.048
PRIMER_RIGHT_11_TM=60.050
PRIMER_LEFT_11_GC_PERCENT=47.826
PRIMER_RIGHT_11_GC_PERCENT=43.478
PRIMER_LEFT_11_SELF_ANY=6.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=2.00
PRIMER_RIGHT_11_SELF_END=2.00
PRIMER_LEFT_11_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, LTR11 LTR from human HTLV-I related endogenous retroviral sequence (HRES-1/1)
PRIMER_PAIR_11_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_11_END_STABILITY=7.9000
PRIMER_RIGHT_11_END_STABILITY=5.1000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_11=364
PRIMER_PAIR_PENALTY_12=0.1027
PRIMER_LEFT_12_PENALTY=0.014220
PRIMER_RIGHT_12_PENALTY=0.088469
PRIMER_LEFT_12_SEQUENCE=ATTACGATGTACATTGGACCAGG
PRIMER_RIGHT_12_SEQUENCE=ACAACCGAGAAATATACTTGGCA
PRIMER_LEFT_12=660,23
PRIMER_RIGHT_12=1153,23
PRIMER_LEFT_12_TM=60.014
PRIMER_RIGHT_12_TM=59.912
PRIMER_LEFT_12_GC_PERCENT=43.478
PRIMER_RIGHT_12_GC_PERCENT=39.130
PRIMER_LEFT_12_SELF_ANY=8.00
PRIMER_RIGHT_12_SELF_ANY=6.00
PRIMER_LEFT_12_SELF_END=3.00
PRIMER_RIGHT_12_SELF_END=3.00
PRIMER_LEFT_12_MISPRIMING_SCORE=12.00, MLT1f (MLT1f subfamily) - consensus sequence
PRIMER_RIGHT_12_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_12_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_12_END_STABILITY=9.7000
PRIMER_RIGHT_12_END_STABILITY=10.0000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_12=494
PRIMER_PAIR_PENALTY_13=0.1029
PRIMER_LEFT_13_PENALTY=0.014220
PRIMER_RIGHT_13_PENALTY=0.088682
PRIMER_LEFT_13_SEQUENCE=ATTACGATGTACATTGGACCAGG
PRIMER_RIGHT_13_SEQUENCE=GGGATACTGAGGTTGGAGGATAC
PRIMER_LEFT_13=660,23
PRIMER_RIGHT_13=1517,23
PRIMER_LEFT_13_TM=60.014
PRIMER_RIGHT_13_TM=60.089
PRIMER_LEFT_13_GC_PERCENT=43.478
PRIMER_RIGHT_13_GC_PERCENT=52.174
PRIMER_LEFT_13_SELF_ANY=8.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=3.00
PRIMER_RIGHT_13_SELF_END=2.00
PRIMER_LEFT_13_MISPRIMING_SCORE=12.00, MLT1f (MLT1f subfamily) - consensus sequence
PRIMER_RIGHT_13_MISPRIMING_SCORE=12.00, MLT1f (MLT1f subfamily) - consensus sequence
PRIMER_PAIR_13_MISPRIMING_SCORE=24.00, MLT1f (MLT1f subfamily) - consensus sequence
PRIMER_LEFT_13_END_STABILITY=9.7000
PRIMER_RIGHT_13_END_STABILITY=5.3000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_13=858
PRIMER_PAIR_PENALTY_14=0.1042
PRIMER_LEFT_14_PENALTY=0.088722
PRIMER_RIGHT_14_PENALTY=0.015502
PRIMER_LEFT_14_SEQUENCE=TAATGAGCCTTTGCTTTTCTTTG
PRIMER_RIGHT_14_SEQUENCE=TTTGTAAAACTTGTGTCGTGTGG
PRIMER_LEFT_14=540,23
PRIMER_RIGHT_14=1179,23
PRIMER_LEFT_14_TM=59.911
PRIMER_RIGHT_14_TM=59.984
PRIMER_LEFT_14_GC_PERCENT=34.783
PRIMER_RIGHT_14_GC_PERCENT=39.130
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_MISPRIMING_SCORE=11.00, MER4C a consensus
PRIMER_RIGHT_14_MISPRIMING_SCORE=11.00, MER34 a consensus
PRIMER_PAIR_14_MISPRIMING_SCORE=21.00, MER34 a consensus
PRIMER_LEFT_14_END_STABILITY=7.3000
PRIMER_RIGHT_14_END_STABILITY=8.2000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_14=640
PRIMER_PAIR_PENALTY_15=0.1048
PRIMER_LEFT_15_PENALTY=0.048411
PRIMER_RIGHT_15_PENALTY=0.056374
PRIMER_LEFT_15_SEQUENCE=TGTAAGCAGCTATCTTCCAGAGG
PRIMER_RIGHT_15_SEQUENCE=CAAGGTGTACACAGTTTCCATGA
PRIMER_LEFT_15=108,23
PRIMER_RIGHT_15=967,23
PRIMER_LEFT_15_TM=60.048
PRIMER_RIGHT_15_TM=59.944
PRIMER_LEFT_15_GC_PERCENT=47.826
PRIMER_RIGHT_15_GC_PERCENT=43.478
PRIMER_LEFT_15_SELF_ANY=6.00
PRIMER_RIGHT_15_SELF_ANY=8.00
PRIMER_LEFT_15_SELF_END=2.00
PRIMER_RIGHT_15_SELF_END=2.00
PRIMER_LEFT_15_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_15_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_15_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_15_END_STABILITY=7.9000
PRIMER_RIGHT_15_END_STABILITY=6.9000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_15=860
PRIMER_PAIR_PENALTY_16=0.1195
PRIMER_LEFT_16_PENALTY=0.082508
PRIMER_RIGHT_16_PENALTY=0.037017
PRIMER_LEFT_16_SEQUENCE=GAATAATGTGGTACCAAGCAAGC
PRIMER_RIGHT_16_SEQUENCE=AGACACAGGTCTCTCCTTCACAG
PRIMER_LEFT_16=1830,23
PRIMER_RIGHT_16=2763,23
PRIMER_LEFT_16_TM=59.917
PRIMER_RIGHT_16_TM=59.963
PRIMER_LEFT_16_GC_PERCENT=43.478
PRIMER_RIGHT_16_GC_PERCENT=52.174
PRIMER_LEFT_16_SELF_ANY=8.00
PRIMER_RIGHT_16_SELF_ANY=6.00
PRIMER_LEFT_16_SELF_END=2.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_16_MISPRIMING_SCORE=12.00, reverse MLT1c (MLT1c subfamily) - consensus sequence
PRIMER_PAIR_16_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_16_END_STABILITY=8.5000
PRIMER_RIGHT_16_END_STABILITY=6.7000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_16=934
PRIMER_PAIR_PENALTY_17=0.1227
PRIMER_LEFT_17_PENALTY=0.014220
PRIMER_RIGHT_17_PENALTY=0.108491
PRIMER_LEFT_17_SEQUENCE=ATTACGATGTACATTGGACCAGG
PRIMER_RIGHT_17_SEQUENCE=TCATTCCACCTGTATATTACCGC
PRIMER_LEFT_17=660,23
PRIMER_RIGHT_17=1037,23
PRIMER_LEFT_17_TM=60.014
PRIMER_RIGHT_17_TM=60.108
PRIMER_LEFT_17_GC_PERCENT=43.478
PRIMER_RIGHT_17_GC_PERCENT=43.478
PRIMER_LEFT_17_SELF_ANY=8.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=3.00
PRIMER_RIGHT_17_SELF_END=2.00
PRIMER_LEFT_17_MISPRIMING_SCORE=12.00, MLT1f (MLT1f subfamily) - consensus sequence
PRIMER_RIGHT_17_MISPRIMING_SCORE=11.00, L1PA11 3'-end of L1 repeat (subfamily L1PA11) - a consensus sequence
PRIMER_PAIR_17_MISPRIMING_SCORE=23.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_17_END_STABILITY=9.7000
PRIMER_RIGHT_17_END_STABILITY=11.1000
PRIMER_PAIR_17_COMPL_ANY=6.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_17=378
PRIMER_PAIR_PENALTY_18=0.1240
PRIMER_LEFT_18_PENALTY=0.108491
PRIMER_RIGHT_18_PENALTY=0.015502
PRIMER_LEFT_18_SEQUENCE=GCGGTAATATACAGGTGGAATGA
PRIMER_RIGHT_18_SEQUENCE=TTTGTAAAACTTGTGTCGTGTGG
PRIMER_LEFT_18=1015,23
PRIMER_RIGHT_18=1179,23
PRIMER_LEFT_18_TM=60.108
PRIMER_RIGHT_18_TM=59.984
PRIMER_LEFT_18_GC_PERCENT=43.478
PRIMER_RIGHT_18_GC_PERCENT=39.130
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=2.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_MISPRIMING_SCORE=11.00, L1MD2 3'-end of L1 repeat (subfamily L1MD2) - a consensus sequence
PRIMER_RIGHT_18_MISPRIMING_SCORE=11.00, MER34 a consensus
PRIMER_PAIR_18_MISPRIMING_SCORE=21.00, reverse MER25 Older LINE1 5' end region - a consensus
PRIMER_LEFT_18_END_STABILITY=6.9000
PRIMER_RIGHT_18_END_STABILITY=8.2000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_18=165
PRIMER_PAIR_PENALTY_19=0.1295
PRIMER_LEFT_19_PENALTY=0.046971
PRIMER_RIGHT_19_PENALTY=0.082508
PRIMER_LEFT_19_SEQUENCE=AACAAGTCGACCATTCTAGTGGA
PRIMER_RIGHT_19_SEQUENCE=GCTTGCTTGGTACCACATTATTC
PRIMER_LEFT_19=1057,23
PRIMER_RIGHT_19=1852,23
PRIMER_LEFT_19_TM=60.047
PRIMER_RIGHT_19_TM=59.917
PRIMER_LEFT_19_GC_PERCENT=43.478
PRIMER_RIGHT_19_GC_PERCENT=43.478
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=8.00
PRIMER_LEFT_19_SELF_END=3.00
PRIMER_RIGHT_19_SELF_END=2.00
PRIMER_LEFT_19_MISPRIMING_SCORE=12.00, reverse MIR2 Repetitive element - a consensus
PRIMER_RIGHT_19_MISPRIMING_SCORE=11.00, L1PA11 3'-end of L1 repeat (subfamily L1PA11) - a consensus sequence
PRIMER_PAIR_19_MISPRIMING_SCORE=21.00, reverse L1MC2 3'-end of L1 repeat (subfamily L1MC2) - a consensus sequence
PRIMER_LEFT_19_END_STABILITY=7.9000
PRIMER_RIGHT_19_END_STABILITY=5.9000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_19=796
=