PRIMER_SEQUENCE_ID=NM_006264
SEQUENCE=CGTCCCTGCAGCCCTCGCCCGGCGCTCCAGTAGCAGGACCCGGTCTCGGGACCAGCCGGTAATATGCACGTGTCACTAGCTGAGGCCCTGGAGGTTCGGGGTGGACCACTTCAGGAGGAAGAAATATGGGCTGTATTAAATCAAAGTGCTGAAAGTCTCCAAGAATTATTCAGAAAAGTAAGCCTAGCTGATCCTGCTGCCCTTGGCTTCATCATTTCTCCATGGTCTCTGCTGTTGCTGCCATCTGGTAGTGTGTCATTTACAGATGAAAATATTTCCAATCAGGATCTTCGAGCATTCACTGCACCAGAGGTTCTTCAAAATCAGTCACTAACTTCTCTCTCAGATGTTGAAAAGATCCACATTTATTCTCTTGGAATGACACTGTATTGGGGGGCTGATTATGAAGTGCCTCAGAGCCAACCTATTAAGCTTGGAGATCATCTCAACAGCATACTGCTTGGAATGTGTGAGGATGTTATTTACGCTCGAGTTTCTGTTCGGACTGTGCTGGATGCTTGCAGTGCCCACATTAGGAATAGCAATTGTGCACCCTCATTTTCCTACGTGAAACACTTGGTAAAACTGGTTCTGGGAAATCTTTCTGGGACAGATCAGCTTTCCTGTAACAGTGAACAAAAGCCTGATCGAAGCCAGGCTATTCGAGATCGATTGCGAGGAAAAGGATTACCAACAGGAAGAAGCTCTACTTCTGATGTACTAGACATACAAAAGCCTCCACTCTCTCATCAGACCTTTCTTAACAAAGGGCTTAGTAAATCTATGGGATTTCTGTCCATCAAAGATACACAAGATGAGAATTATTTCAAGGACATTTTATCAGATAATTCTGGACGTGAAGATTCTGAAAATACATTCTCCCCTTACCAGTTCAAAACTAGTGGCCCAGAAAAAAAACCCATCCCTGGCATTGATGTGCTTTCTAAGAAGAAGATCTGGGCTTCATCCATGGACTTGCTTTGTACAGCTGACAGAGACTTCTCTTCAGGAGAGACTGCCACATATCGTCGTTGTCACCCTGAGGCAGTAACAGTGCGGACTTCAACTACTCCTAGAAAAAAGGAGGCAAGATACTCAGATGGAAGTATAGCCTTGGATATCTTTGGCCCTCAGAAAATGGATCCAATATATCACACTCGAGAATTGCCCACCTCCTCAGCAATATCAAGTGCTTTGGACCGAATCCGAGAGAGACAAAAGAAACTTCAGGTTCTGAGGGAAGCCATGAATGTAGAAGAACCAGTTCGAAGATACAAAACTTATCATGGTGATGTCTTTAGTACCTCCAGTGAAAGTCCATCTATTATTTCCTCTGAATCAGATTTCAGACAAGTGAGAAGAAGTGAAGCCTCAAAGAGGTTTGAATCCAGCAGTGGTCTCCCAGGGGTAGATGAAACCTTAAGTCAAGGCCAGTCACAGAGACCGAGCAGACAATATGAAACACCCTTTGAAGGCAACTTAATTAATCAAGAGATCATGCTAAAACGGCAAGAGGAAGAACTGATGCAGCTACAAGCCAAAATGGCCCTTAGACAGTCTCGGTTGAGCCTATATCCAGGAGACACAATCAAAGCGTCCATGCTTGACATCACCAGGGATCCGTTAAGAGAAATTGCCCTAGAAACAGCCATGACTCAAAGAAAACTGAGGAATTTCTTTGGCCCTGAGTTTGTGAAAATGACAATTGAACCATTTATATCTTTGGATTTGCCACGGTCTATTCTTACTAAGAAAGGGAAGAATGAGGATAACCGAAGGAAAGTAAACATAATGCTTCTGAACGGGCAAAGACTGGAACTGACCTGTGATACCAAAACTATATGTAAAGATGTGTTTGATATGGTTGTGGCACATATTGGCTTAGTAGAGCATCATTTGTTTGCTTTAGCTACCCTCAAAGATAATGAATATTTCTTTGTTGATCCTGACTTAAAATTAACCAAAGTGGCCCCAGAGGGATGGAAAGAAGAACCAAAGAAAAAGACCAAAGCCACTGTTAATTTTACTTTGTTTTTCAGAATTAAATTTTTTATGGATGATGTTAGTCTAATACAACATACTCTGACGTGTCATCAGTATTACCTTCAGCTTCGAAAAGATATTTTGGAGGAAAGGATGCACTGTGATGATGAGACTTCCTTATTGCTGGCATCCTTGGCTCTCCAGGCTGAGTATGGAGATTATCAACCAGAGGTTCATGGTGTGTCTTACTTTAGAATGGAGCACTATTTGCCCGCCAGAGTGATGGAGAAACTTGATTTATCCTATATCAAAGAAGAGTTACCCAAATTGCATAATACCTATGTGGGAGCTTCTGAAAAAGAGACAGAGTTAGAATTTTTAAAGGTCTGCCAAAGACTGACAGAATATGGAGTTCATTTTCACCGAGTGCACCCTGAGAAGAAGTCACAAACAGGAATATTGCTTGGAGTCTGTTCTAAAGGTGTCCTTGTGTTTGAAGTTCACAATGGAGTGCGCACATTGGTCCTTCGCTTTCCATGGAGGGAAACCAAGAAAATATCTTTTTCTAAAAAGAAAATCACATTGCAAAATACATCAGATGGAATAAAACATGGCTTCCAGACAGACAACAGTAAGATATGCCAGTACCTGCTGCACCTCTGCTCTTACCAGCATAAGTTCCAGCTACAGATGAGAGCAAGACAGAGCAACCAAGATGCCCAAGATATTGAGAGAGCTTCGTTTAGGAGCCTGAATCTCCAAGCAGAGTCTGTTAGAGGATTTAATATGGGACGAGCAATCAGCACTGGCAGTCTGGCCAGCAGCACCCTCAACAAACTTGCTGTTCGACCTTTATCAGTTCAAGCTGAGATTCTGAAGAGGCTATCCTGCTCAGAGCTGTCGCTTTACCAGCCATTGCAAAACAGTTCAAAAGAGAAGAATGACAAAGCTTCATGGGAGGAAAAGCCTAGAGAGATGAGTAAATCATACCATGATCTCAGTCAGGCCTCTCTCTATCCACATCGGAAAAATGTCATTGTTAACATGGAACCCCCACCACAAACCGTTGCAGAGTTGGTGGGAAAACCTTCTCACCAGATGTCAAGATCTGATGCAGAATCTTTGGCAGGAGTGACAAAACTTAATAATTCAAAGTCTGTTGCGAGTTTAAATAGAAGTCCTGAAAGGAGGAAACATGAATCAGACTCCTCATCCATTGAAGACCCTGGGCAAGCATATGTTCTAGATGTGCTACACAAAAGATGGAGCATAGTATCTTCACCAGAAAGGGAGATCACCTTAGTGAACCTGAAAAAAGATGCAAAGTATGGCTTGGGATTTCAAATTATTGGTGGGGAGAAGATGGGAAGACTGGACCTAGGCATATTTATCAGTTCAGTTGCCCCTGGAGGACCAGCTGACTTGGATGGATGCTTGAAGCCAGGAGACCGTTTGATATCTGTGAATAGTGTGAGTCTGGAGGGAGTCAGCCACCATGCTGCAATTGAAATTTTGCAAAATGCACCTGAAGATGTGACACTTGTTATCTCTCAGCCAAAAGAAAAGATATCCAAAGTGCCTTCTACTCCTGTGCATCTCACCAATGAGATGAAAAACTACATGAAGAAATCTTCCTACATGCAAGACAGTGCTATAGATTCTTCTTCCAAGGATCACCACTGGTCACGTGGTACCCTGAGGCACATCTCGGAGAACTCCTTTGGGCCATCTGGGGGCCTGCGGGAAGGAAGCCTGAGTTCTCAAGATTCCAGGACTGAGAGTGCCAGCTTGTCTCAAAGCCAGGTCAATGGTTTCTTTGCCAGCCATTTAGGTGACCAAACCTGGCAGGAATCACAGCATGGCAGCCCTTCCCCATCTGTAATATCCAAAGCCACCGAGAAAGAGACTTTCACTGATAGTAACCAAAGCAAAACTAAAAAGCCAGGCATTTCTGATGTAACTGATTACTCAGACCGTGGAGATTCAGACATGGATGAAGCCACTTACTCCAGCAGTCAGGATCATCAAACACCAAAACAGGAATCTTCCTCTTCAGTGAATACATCCAACAAGATGAATTTTAAAACTTTTTCTTCATCACCTCCTAAGCCTGGAGATATCTTTGAGGTTGAACTGGCTAAAAATGATAACAGCTTGGGGATAAGTGTCACGGGAGGTGTGAATACGAGTGTCAGACATGGTGGCATTTATGTGAAAGCTGTTATTCCCCAGGGAGCAGCAGAGTCTGATGGTAGAATTCACAAAGGTGATCGCGTCCTAGCTGTCAATGGAGTTAGTCTAGAAGGAGCCACCCATAAGCAAGCTGTGGAAACACTGAGAAATACAGGACAGGTGGTTCATCTGTTATTAGAAAAGGGACAATCTCCAACATCTAAAGAACATGTCCCGGTAACCCCACAGTGTACCCTTTCAGATCAGAATGCCCAAGGTCAAGGCCCAGAAAAAGTGAAGAAAACAACTCAGGTCAAAGACTACAGCTTTGTCACTGAAGAAAATACATTTGAGGTAAAATTATTTAAAAATAGCTCAGGTCTAGGATTCAGTTTTTCTCGAGAAGATAATCTTATACCGGAGCAAATTAATGCCAGCATAGTAAGGGTTAAAAAGCTCTTTCCTGGACAGCCAGCAGCAGAAAGTGGAAAAATTGATGTAGGAGATGTTATCTTGAAAGTGAATGGAGCCTCTTTGAAAGGACTATCTCAGCAGGAAGTCATATCTGCTCTCAGGGGAACTGCTCCAGAAGTATTCTTGCTTCTCTGCAGACCTCCACCTGGTGTGCTACCGGAAATTGATACTGCGCTTTTGACCCCACTTCAGTCTCCAGCACAAGTACTTCCAAACAGCAGTAAAGACTCTTCTCAGCCATCATGTGTGGAGCAAAGCACCAGCTCAGATGAAAATGAAATGTCAGACAAAAGCAAAAAACAGTGCAAGTCCCCATCCAGAAGAGACAGTTACAGTGACAGCAGTGGGAGTGGAGAAGATGACTTAGTGACAGCTCCAGCAAACATATCAAATTCGACCTGGAGTTCAGCTTTGCATCAGACTCTAAGCAACATGGTATCACAGGCACAGAGTCATCATGAAGCACCCAAGAGTCAAGAAGATACCATTTGTACCATGTTTTACTATCCTCAGAAAATTCCCAATAAACCAGAGTTTGAGGACAGTAATCCTTCCCCTCTACCACCGGATATGGCTCCTGGGCAGAGTTATCAACCCCAATCAGAATCTGCTTCCTCTAGTTCGATGGATAAGTATCATATACATCACATTTCTGAACCAACTAGACAAGAAAACTGGACACCTTTGAAAAATGACTTGGAAAATCACCTTGAAGACTTTGAACTGGAAGTAGAACTCCTCATTACCCTAATTAAATCAGAAAAAGGAAGCCTGGGTTTTACAGTAACCAAAGGCAATCAGAGAATTGGTTGTTATGTTCATGATGTCATACAGGATCCAGCCAAAAGTGATGGAAGGCTAAAACCTGGGGACCGGCTCATAAAGGTTAATGATACAGATGTTACTAATATGACTCATACAGATGCAGTTAATCTGCTCCGGGCTGCATCCAAAACAGTCAGATTAGTTATTGGACGAGTTCTAGAATTACCCAGAATACCAATGTTGCCTCATTTGCTACCGGACATAACACTAACGTGCAACAAAGAGGAGTTGGGTTTTTCCTTATGTGGAGGTCATGACAGCCTTTATCAAGTGGTATATATTAGTGATATTAATCCAAGGTCCGTCGCAGCCATTGAGGGTAATCTCCAGCTATTAGATGTCATCCATTATGTGAACGGAGTCAGCACACAAGGAATGACCTTGGAGGAAGTTAACAGAGCATTAGACATGTCACTTCCTTCATTGGTATTGAAAGCAACAAGAAATGATCTTCCAGTGGTCCCCAGCTCAAAGAGGTCTGCTGTTTCAGCTCCAAAGTCAACCAAAGGCAATGGTTCCTACAGTGTGGGGTCTTGCAGCCAGCCTGCCCTCACTCCTAATGATTCATTCTCCACGGTTGCTGGGGAAGAAATAAATGAAATATCGTACCCCAAAGGAAAATGTTCTACTTATCAGATAAAGGGATCACCAAACTTGACTCTGCCCAAAGAATCTTATATACAAGAAGATGACATTTATGATGATTCCCAAGAAGCTGAAGTTATCCAGTCTCTGCTGGATGTTGTGGATGAGGAAGCCCAGAATCTTTTAAACGAAAATAATGCAGCAGGATACTCCTGTGGTCCAGGTACATTAAAGATGAATGGGAAGTTATCAGAAGAGAGAACAGAAGATACAGACTGCGATGGTTCACCTTTACCTGAGTATTTTACTGAGGCCACCAAAATGAATGGCTGTGAAGAATATTGTGAAGAAAAAGTAAAAAGTGAAAGCTTAATTCAGAAGCCACAAGAAAAGAAGACTGATGATGATGAAATAACATGGGGAAATGATGAGTTGCCAATAGAGAGAACAAACCATGAAGATTCTGATAAAGATCATTCCTTTCTGACAAACGATGAGCTCGCTGTACTCCCTGTCGTCAAAGTGCTTCCCTCTGGTAAATACACGGGTGCCAACTTAAAATCAGTCATTCGAGTCCTGCGGGGTTTGCTAGATCAAGGAATTCCTTCTAAGGAGCTGGAGAATCTTCAAGAATTAAAACCTTTGGATCAGTGTCTAATTGGGCAAACTAAGGAAAACAGAAGGAAGAACAGATATAAAAATATACTTCCCTATGATGCTACAAGAGTGCCTCTTGGAGATGAAGGTGGCTATATCAATGCCAGCTTCATTAAGATACCAGTTGGGAAAGAAGAGTTCGTTTACATTGCCTGCCAAGGACCACTGCCTACAACTGTTGGAGACTTCTGGCAGATGATTTGGGAGCAAAAATCCACAGTGATAGCCATGATGACTCAAGAAGTAGAAGGAGAAAAAATCAAATGCCAGCGCTATTGGCCCAACATCCTAGGCAAAACAACAATGGTCAGCAACAGACTTCGACTGGCTCTTGTGAGAATGCAGCAGCTGAAGGGCTTTGTGGTGAGGGCAATGACCCTTGAAGATATTCAGACCAGAGAGGTGCGCCATATTTCTCATCTGAATTTCACTGCCTGGCCAGACCATGATACACCTTCTCAACCAGATGATCTGCTTACTTTTATCTCCTACATGAGACACATCCACAGATCAGGCCCAATCATTACGCACTGCAGTGCTGGCATTGGACGTTCAGGGACCCTGATTTGCATAGATGTGGTTCTGGGATTAATCAGTCAGGATCTTGATTTTGACATCTCTGATTTGGTGCGCTGCATGAGACTACAAAGACACGGAATGGTTCAGACAGAGGATCAATATATTTTCTGCTATCAAGTCATCCTTTATGTCCTGACACGTCTTCAAGCAGAAGAAGAGCAAAAACAGCAGCCTCAGCTTCTGAAGTGACATGAAAAGAGCCTCTGGATGCATTTCCATTTCTCTCCTTAACCTCCAGCAGACTCCTGCTCTCTATCCAAAATAAAGATCACAGAGCAGCAAGTTCATACAACATGCATGTTCTCCTCTATCTTAGAGGGGTATTCTTCTTGAAAATAAAAAATATTGAAATGCTGTATTTTTACAGCTACTTTAACCTATGATAATTATTTACAAAATTTTAACACTAACCAAACAATGCAGATCTTAGGGATGATTAAAGGCAGCATTTGATGATAGCAGACATTGTTACAAGGACATGGTGAGTCTATTTTTAATGCACCAATCTTGTTTATAGCAAAAATGTTTTCCAATATTTTAATAAAGTAGTTATTTTATAGGGGATACTTGAAACCAGTATTTAAGCTTTAAATGACAGTAATATTGGCATAGAAAAAAGTAGCAAATGTTTACTGTATCAATTTCTAATGTTTACTATATAGAATTTCCTGTAATATATTTATATACTTTTTCATGAAAATGGAGTTATCAGTTATCTGTTTGTTACTGCATCATCTGTTTGTAATCATTATCTCACTTTGTAAATAAAAACACACCTTAAAACATG
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 94269, GC content failed 2524, low tm 51436, high tm 18236, high any compl 3, high end compl 29, high repeat similarity 40, long poly-x seq 168, ok 21833
PRIMER_RIGHT_EXPLAIN=considered 94303, GC content failed 2505, low tm 52098, high tm 17708, high any compl 3, high end compl 15, high repeat similarity 14, long poly-x seq 166, ok 21794
PRIMER_PAIR_EXPLAIN=considered 1466, unacceptable product size 1408, high end compl 9, ok 49
PRIMER_PAIR_PENALTY=0.0107
PRIMER_LEFT_PENALTY=0.001065
PRIMER_RIGHT_PENALTY=0.009608
PRIMER_LEFT_SEQUENCE=ACTTAGTGACAGCTCCAGCAAAC
PRIMER_RIGHT_SEQUENCE=GACCACTGGAAGATCATTTCTTG
PRIMER_LEFT=4990,23
PRIMER_RIGHT=5915,23
PRIMER_LEFT_TM=59.999
PRIMER_RIGHT_TM=59.990
PRIMER_LEFT_GC_PERCENT=47.826
PRIMER_RIGHT_GC_PERCENT=43.478
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_MISPRIMING_SCORE=12.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=926
PRIMER_PAIR_PENALTY_1=0.0154
PRIMER_LEFT_1_PENALTY=0.009608
PRIMER_RIGHT_1_PENALTY=0.005764
PRIMER_LEFT_1_SEQUENCE=CAAGAAATGATCTTCCAGTGGTC
PRIMER_RIGHT_1_SEQUENCE=TATCCTGCTGCATTATTTTCGTT
PRIMER_LEFT_1=5893,23
PRIMER_RIGHT_1=6247,23
PRIMER_LEFT_1_TM=59.990
PRIMER_RIGHT_1_TM=60.006
PRIMER_LEFT_1_GC_PERCENT=43.478
PRIMER_RIGHT_1_GC_PERCENT=34.783
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_MISPRIMING_SCORE=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_1_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=355
PRIMER_PAIR_PENALTY_2=0.0167
PRIMER_LEFT_2_PENALTY=0.005048
PRIMER_RIGHT_2_PENALTY=0.011619
PRIMER_LEFT_2_SEQUENCE=GAAGTCCTGAAAGGAGGAAACAT
PRIMER_RIGHT_2_SEQUENCE=TGAATCTCCACGGTCTGAGTAAT
PRIMER_LEFT_2=3157,23
PRIMER_RIGHT_2=3968,23
PRIMER_LEFT_2_TM=59.995
PRIMER_RIGHT_2_TM=60.012
PRIMER_LEFT_2_GC_PERCENT=43.478
PRIMER_RIGHT_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_2_MISPRIMING_SCORE=12.00, MER21B a consensus
PRIMER_PAIR_2_MISPRIMING_SCORE=24.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=5.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=812
PRIMER_PAIR_PENALTY_3=0.0167
PRIMER_LEFT_3_PENALTY=0.005048
PRIMER_RIGHT_3_PENALTY=0.011619
PRIMER_LEFT_3_SEQUENCE=GAAGTCCTGAAAGGAGGAAACAT
PRIMER_RIGHT_3_SEQUENCE=TCACAGATATCAAACGGTCTCCT
PRIMER_LEFT_3=3157,23
PRIMER_RIGHT_3=3447,23
PRIMER_LEFT_3_TM=59.995
PRIMER_RIGHT_3_TM=60.012
PRIMER_LEFT_3_GC_PERCENT=43.478
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_3_MISPRIMING_SCORE=10.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_3_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=291
PRIMER_PAIR_PENALTY_4=0.0224
PRIMER_LEFT_4_PENALTY=0.005764
PRIMER_RIGHT_4_PENALTY=0.016658
PRIMER_LEFT_4_SEQUENCE=AACGAAAATAATGCAGCAGGATA
PRIMER_RIGHT_4_SEQUENCE=CGAATGACTGATTTTAAGTTGGC
PRIMER_LEFT_4=6225,23
PRIMER_RIGHT_4=6610,23
PRIMER_LEFT_4_TM=60.006
PRIMER_RIGHT_4_TM=60.017
PRIMER_LEFT_4_GC_PERCENT=34.783
PRIMER_RIGHT_4_GC_PERCENT=39.130
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_4_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_4_END_STABILITY=7.1000
PRIMER_RIGHT_4_END_STABILITY=10.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=386
PRIMER_PAIR_PENALTY_5=0.0232
PRIMER_LEFT_5_PENALTY=0.011619
PRIMER_RIGHT_5_PENALTY=0.011619
PRIMER_LEFT_5_SEQUENCE=AGGAGACCGTTTGATATCTGTGA
PRIMER_RIGHT_5_SEQUENCE=TGAATCTCCACGGTCTGAGTAAT
PRIMER_LEFT_5=3425,23
PRIMER_RIGHT_5=3968,23
PRIMER_LEFT_5_TM=60.012
PRIMER_RIGHT_5_TM=60.012
PRIMER_LEFT_5_GC_PERCENT=43.478
PRIMER_RIGHT_5_GC_PERCENT=43.478
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=2.00
PRIMER_LEFT_5_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_5_MISPRIMING_SCORE=12.00, MER21B a consensus
PRIMER_PAIR_5_MISPRIMING_SCORE=24.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_5_END_STABILITY=6.7000
PRIMER_RIGHT_5_END_STABILITY=5.6000
PRIMER_PAIR_5_COMPL_ANY=7.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_5=544
PRIMER_PAIR_PENALTY_6=0.0246
PRIMER_LEFT_6_PENALTY=0.001065
PRIMER_RIGHT_6_PENALTY=0.023556
PRIMER_LEFT_6_SEQUENCE=ACTTAGTGACAGCTCCAGCAAAC
PRIMER_RIGHT_6_SEQUENCE=TGTTGCACGTTAGTGTTATGTCC
PRIMER_LEFT_6=4990,23
PRIMER_RIGHT_6=5673,23
PRIMER_LEFT_6_TM=59.999
PRIMER_RIGHT_6_TM=59.976
PRIMER_LEFT_6_GC_PERCENT=47.826
PRIMER_RIGHT_6_GC_PERCENT=43.478
PRIMER_LEFT_6_SELF_ANY=5.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_6_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_6_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_6_END_STABILITY=7.0000
PRIMER_RIGHT_6_END_STABILITY=7.9000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_6=684
PRIMER_PAIR_PENALTY_7=0.0263
PRIMER_LEFT_7_PENALTY=0.009608
PRIMER_RIGHT_7_PENALTY=0.016658
PRIMER_LEFT_7_SEQUENCE=CAAGAAATGATCTTCCAGTGGTC
PRIMER_RIGHT_7_SEQUENCE=CGAATGACTGATTTTAAGTTGGC
PRIMER_LEFT_7=5893,23
PRIMER_RIGHT_7=6610,23
PRIMER_LEFT_7_TM=59.990
PRIMER_RIGHT_7_TM=60.017
PRIMER_LEFT_7_GC_PERCENT=43.478
PRIMER_RIGHT_7_GC_PERCENT=39.130
PRIMER_LEFT_7_SELF_ANY=5.00
PRIMER_RIGHT_7_SELF_ANY=5.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=2.00
PRIMER_LEFT_7_MISPRIMING_SCORE=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_7_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_7_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_7_END_STABILITY=7.9000
PRIMER_RIGHT_7_END_STABILITY=10.0000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_7=718
PRIMER_PAIR_PENALTY_8=0.0293
PRIMER_LEFT_8_PENALTY=0.023556
PRIMER_RIGHT_8_PENALTY=0.005764
PRIMER_LEFT_8_SEQUENCE=GGACATAACACTAACGTGCAACA
PRIMER_RIGHT_8_SEQUENCE=TATCCTGCTGCATTATTTTCGTT
PRIMER_LEFT_8=5651,23
PRIMER_RIGHT_8=6247,23
PRIMER_LEFT_8_TM=59.976
PRIMER_RIGHT_8_TM=60.006
PRIMER_LEFT_8_GC_PERCENT=43.478
PRIMER_RIGHT_8_GC_PERCENT=34.783
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=5.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_8_MISPRIMING_SCORE=24.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_8_END_STABILITY=7.0000
PRIMER_RIGHT_8_END_STABILITY=8.4000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_8=597
PRIMER_PAIR_PENALTY_9=0.0309
PRIMER_LEFT_9_PENALTY=0.016658
PRIMER_RIGHT_9_PENALTY=0.014220
PRIMER_LEFT_9_SEQUENCE=GCCAACTTAAAATCAGTCATTCG
PRIMER_RIGHT_9_SEQUENCE=CTATCACTGTGGATTTTTGCTCC
PRIMER_LEFT_9=6588,23
PRIMER_RIGHT_9=6921,23
PRIMER_LEFT_9_TM=60.017
PRIMER_RIGHT_9_TM=60.014
PRIMER_LEFT_9_GC_PERCENT=39.130
PRIMER_RIGHT_9_GC_PERCENT=43.478
PRIMER_LEFT_9_SELF_ANY=5.00
PRIMER_RIGHT_9_SELF_ANY=5.00
PRIMER_LEFT_9_SELF_END=2.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_MISPRIMING_SCORE=12.00, MLT1b (MLT1b subfamily) - consensus sequence
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, reverse MER25 Older LINE1 5' end region - a consensus
PRIMER_PAIR_9_MISPRIMING_SCORE=21.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_LEFT_9_END_STABILITY=8.6000
PRIMER_RIGHT_9_END_STABILITY=9.4000
PRIMER_PAIR_9_COMPL_ANY=6.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_9=334
PRIMER_PAIR_PENALTY_10=0.0332
PRIMER_LEFT_10_PENALTY=0.023556
PRIMER_RIGHT_10_PENALTY=0.009608
PRIMER_LEFT_10_SEQUENCE=GGACATAACACTAACGTGCAACA
PRIMER_RIGHT_10_SEQUENCE=GACCACTGGAAGATCATTTCTTG
PRIMER_LEFT_10=5651,23
PRIMER_RIGHT_10=5915,23
PRIMER_LEFT_10_TM=59.976
PRIMER_RIGHT_10_TM=59.990
PRIMER_LEFT_10_GC_PERCENT=43.478
PRIMER_RIGHT_10_GC_PERCENT=43.478
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=5.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=3.00
PRIMER_LEFT_10_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_10_MISPRIMING_SCORE=12.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_10_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_10_END_STABILITY=7.0000
PRIMER_RIGHT_10_END_STABILITY=7.0000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_10=265
PRIMER_PAIR_PENALTY_11=0.0352
PRIMER_LEFT_11_PENALTY=0.017590
PRIMER_RIGHT_11_PENALTY=0.017590
PRIMER_LEFT_11_SEQUENCE=AAGGATTACCAACAGGAAGAAGC
PRIMER_RIGHT_11_SEQUENCE=GGCCTTGACTTAAGGTTTCATCT
PRIMER_LEFT_11=682,23
PRIMER_RIGHT_11=1431,23
PRIMER_LEFT_11_TM=60.018
PRIMER_RIGHT_11_TM=60.018
PRIMER_LEFT_11_GC_PERCENT=43.478
PRIMER_RIGHT_11_GC_PERCENT=43.478
PRIMER_LEFT_11_SELF_ANY=2.00
PRIMER_RIGHT_11_SELF_ANY=7.00
PRIMER_LEFT_11_SELF_END=2.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_11_MISPRIMING_SCORE=24.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_11_END_STABILITY=8.2000
PRIMER_RIGHT_11_END_STABILITY=6.6000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_11=750
PRIMER_PAIR_PENALTY_12=0.0402
PRIMER_LEFT_12_PENALTY=0.023556
PRIMER_RIGHT_12_PENALTY=0.016658
PRIMER_LEFT_12_SEQUENCE=GGACATAACACTAACGTGCAACA
PRIMER_RIGHT_12_SEQUENCE=CGAATGACTGATTTTAAGTTGGC
PRIMER_LEFT_12=5651,23
PRIMER_RIGHT_12=6610,23
PRIMER_LEFT_12_TM=59.976
PRIMER_RIGHT_12_TM=60.017
PRIMER_LEFT_12_GC_PERCENT=43.478
PRIMER_RIGHT_12_GC_PERCENT=39.130
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=5.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=2.00
PRIMER_LEFT_12_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_12_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_12_MISPRIMING_SCORE=24.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_12_END_STABILITY=7.0000
PRIMER_RIGHT_12_END_STABILITY=10.0000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_12=960
PRIMER_PAIR_PENALTY_13=0.0433
PRIMER_LEFT_13_PENALTY=0.009608
PRIMER_RIGHT_13_PENALTY=0.033701
PRIMER_LEFT_13_SEQUENCE=CAAGAAATGATCTTCCAGTGGTC
PRIMER_RIGHT_13_SEQUENCE=TTTTCCTTTGGGGTACGATATTT
PRIMER_LEFT_13=5893,23
PRIMER_RIGHT_13=6074,23
PRIMER_LEFT_13_TM=59.990
PRIMER_RIGHT_13_TM=59.966
PRIMER_LEFT_13_GC_PERCENT=43.478
PRIMER_RIGHT_13_GC_PERCENT=34.783
PRIMER_LEFT_13_SELF_ANY=5.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=2.00
PRIMER_LEFT_13_MISPRIMING_SCORE=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_13_MISPRIMING_SCORE=11.00, L1PA15 3'-end of L1 repeat (subfamily L1PA15) - a consensus sequence
PRIMER_PAIR_13_MISPRIMING_SCORE=20.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_LEFT_13_END_STABILITY=7.9000
PRIMER_RIGHT_13_END_STABILITY=6.2000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_13=182
PRIMER_PAIR_PENALTY_14=0.0463
PRIMER_LEFT_14_PENALTY=0.011619
PRIMER_RIGHT_14_PENALTY=0.034654
PRIMER_LEFT_14_SEQUENCE=ATTACTCAGACCGTGGAGATTCA
PRIMER_RIGHT_14_SEQUENCE=GAAGCAAGAATACTTCTGGAGCA
PRIMER_LEFT_14=3946,23
PRIMER_RIGHT_14=4758,23
PRIMER_LEFT_14_TM=60.012
PRIMER_RIGHT_14_TM=60.035
PRIMER_LEFT_14_GC_PERCENT=43.478
PRIMER_RIGHT_14_GC_PERCENT=43.478
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=2.00
PRIMER_RIGHT_14_SELF_END=3.00
PRIMER_LEFT_14_MISPRIMING_SCORE=11.00, MER21B a consensus
PRIMER_RIGHT_14_MISPRIMING_SCORE=11.00, LTR4 LTR from human endogenous retrovirus ERV3, POL-ENV-3'LTR region
PRIMER_PAIR_14_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_14_END_STABILITY=6.9000
PRIMER_RIGHT_14_END_STABILITY=8.2000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_14=813
PRIMER_PAIR_PENALTY_15=0.0505
PRIMER_LEFT_15_PENALTY=0.005048
PRIMER_RIGHT_15_PENALTY=0.045432
PRIMER_LEFT_15_SEQUENCE=GAAGTCCTGAAAGGAGGAAACAT
PRIMER_RIGHT_15_SEQUENCE=CCCTCCAGACTCACACTATTCAC
PRIMER_LEFT_15=3157,23
PRIMER_RIGHT_15=3466,23
PRIMER_LEFT_15_TM=59.995
PRIMER_RIGHT_15_TM=60.045
PRIMER_LEFT_15_GC_PERCENT=43.478
PRIMER_RIGHT_15_GC_PERCENT=52.174
PRIMER_LEFT_15_SELF_ANY=5.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=2.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_15_MISPRIMING_SCORE=11.00, MER39 a consensus
PRIMER_PAIR_15_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_15_END_STABILITY=6.6000
PRIMER_RIGHT_15_END_STABILITY=6.7000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_15=310
PRIMER_PAIR_PENALTY_16=0.0545
PRIMER_LEFT_16_PENALTY=0.044904
PRIMER_RIGHT_16_PENALTY=0.009608
PRIMER_LEFT_16_SEQUENCE=TAGTGACAGCTCCAGCAAACATA
PRIMER_RIGHT_16_SEQUENCE=GACCACTGGAAGATCATTTCTTG
PRIMER_LEFT_16=4993,23
PRIMER_RIGHT_16=5915,23
PRIMER_LEFT_16_TM=59.955
PRIMER_RIGHT_16_TM=59.990
PRIMER_LEFT_16_GC_PERCENT=43.478
PRIMER_RIGHT_16_GC_PERCENT=43.478
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=5.00
PRIMER_LEFT_16_SELF_END=2.00
PRIMER_RIGHT_16_SELF_END=3.00
PRIMER_LEFT_16_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_16_MISPRIMING_SCORE=12.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_16_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_16_END_STABILITY=5.6000
PRIMER_RIGHT_16_END_STABILITY=7.0000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_16=923
PRIMER_PAIR_PENALTY_17=0.0565
PRIMER_LEFT_17_PENALTY=0.044904
PRIMER_RIGHT_17_PENALTY=0.011619
PRIMER_LEFT_17_SEQUENCE=TTCCTACATGCAAGACAGTGCTA
PRIMER_RIGHT_17_SEQUENCE=TGAATCTCCACGGTCTGAGTAAT
PRIMER_LEFT_17=3614,23
PRIMER_RIGHT_17=3968,23
PRIMER_LEFT_17_TM=59.955
PRIMER_RIGHT_17_TM=60.012
PRIMER_LEFT_17_GC_PERCENT=43.478
PRIMER_RIGHT_17_GC_PERCENT=43.478
PRIMER_LEFT_17_SELF_ANY=6.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=2.00
PRIMER_RIGHT_17_SELF_END=2.00
PRIMER_LEFT_17_MISPRIMING_SCORE=12.00, MLT1f (MLT1f subfamily) - consensus sequence
PRIMER_RIGHT_17_MISPRIMING_SCORE=12.00, MER21B a consensus
PRIMER_PAIR_17_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_17_END_STABILITY=7.5000
PRIMER_RIGHT_17_END_STABILITY=5.6000
PRIMER_PAIR_17_COMPL_ANY=6.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_17=355
PRIMER_PAIR_PENALTY_18=0.0571
PRIMER_LEFT_18_PENALTY=0.045432
PRIMER_RIGHT_18_PENALTY=0.011619
PRIMER_LEFT_18_SEQUENCE=GTGAATAGTGTGAGTCTGGAGGG
PRIMER_RIGHT_18_SEQUENCE=TGAATCTCCACGGTCTGAGTAAT
PRIMER_LEFT_18=3444,23
PRIMER_RIGHT_18=3968,23
PRIMER_LEFT_18_TM=60.045
PRIMER_RIGHT_18_TM=60.012
PRIMER_LEFT_18_GC_PERCENT=52.174
PRIMER_RIGHT_18_GC_PERCENT=43.478
PRIMER_LEFT_18_SELF_ANY=3.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=2.00
PRIMER_LEFT_18_MISPRIMING_SCORE=11.00, L1MC2 3'-end of L1 repeat (subfamily L1MC2) - a consensus sequence
PRIMER_RIGHT_18_MISPRIMING_SCORE=12.00, MER21B a consensus
PRIMER_PAIR_18_MISPRIMING_SCORE=22.00, MER21B a consensus
PRIMER_LEFT_18_END_STABILITY=9.4000
PRIMER_RIGHT_18_END_STABILITY=5.6000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_18=525
PRIMER_PAIR_PENALTY_19=0.0573
PRIMER_LEFT_19_PENALTY=0.023556
PRIMER_RIGHT_19_PENALTY=0.033701
PRIMER_LEFT_19_SEQUENCE=GGACATAACACTAACGTGCAACA
PRIMER_RIGHT_19_SEQUENCE=TTTTCCTTTGGGGTACGATATTT
PRIMER_LEFT_19=5651,23
PRIMER_RIGHT_19=6074,23
PRIMER_LEFT_19_TM=59.976
PRIMER_RIGHT_19_TM=59.966
PRIMER_LEFT_19_GC_PERCENT=43.478
PRIMER_RIGHT_19_GC_PERCENT=34.783
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=2.00
PRIMER_LEFT_19_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_19_MISPRIMING_SCORE=11.00, L1PA15 3'-end of L1 repeat (subfamily L1PA15) - a consensus sequence
PRIMER_PAIR_19_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_19_END_STABILITY=7.0000
PRIMER_RIGHT_19_END_STABILITY=6.2000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_19=424
=