PRIMER_SEQUENCE_ID=NM_007026
SEQUENCE=GTGGCCGCGCAGGAGGACGGAGCCCTAACCGCAACCCGCGCCGCGCCGCGCCGATTTGATTTGTATCCACTGTCACCAGCACTGCTCACTTAGGACTTTCTGGATCCAGACCCAGGCAGCGCACACTGGACTCTTGAGGAAGAAGGAGACTCTAATTTTGGATTCCTTGGTGGAGGAAAATAAAACACTCTGGTCTTGCCGCCAACGATGCAAGTGTGACTGCTGGCGTCTTCATGAGCTCCAGAGGTCACAGCACGCTACCAAGGACTCTCATGGCCCCTCGGATGATTTCCGAGGGAGACATAGGAGGCATTGCTCAAATCACCTCCTCTCTATTCCTGGGCAGAGGCAGTGTGGCCTCCAATCGGCACCTCCTCCAGGCTCGTGGCATCACCTGCATTGTTAATGCTACCATTGAGATCCCTAATTTCAACTGGCCCCAATTTGAGTATGTTAAAGTGCCTCTGGCTGACATGCCGCATGCCCCCATTGGACTGTACTTTGACACCGTGGCTGACAAGATCCACAGTGTGAGCAGGAAGCACGGGGCCACCTTGGTGCACTGTGCTGCAGGGGTGAGCCGCTCAGCCACGCTGTGTATCGCGTACCTGATGAAATTCCACAACGTGTGCCTGCTGGAGGCATACAACTGGGTGAAAGCCCGGCGACCTGTCATCAGGCCCAACGTAGGCTTCTGGAGGCAACTGATAGACTACGAGCGCCAGCTCTTTGGGAAGTCGACAGTTAAAATGGTACAGACACCTTATGGCATAGTTCCCGACGTCTATGAGAAGGAGTCCCGACACCTGATGCCTTACTGGGGGATTTAGTGCCACTGAAGCCTGCGTCAGCAGCCCGAGCGGGGCCGGCATCTGCTCCCCGCCGTCTGCTCCCTCTCCACTCTCTTCTCAAATGGCTGACTTCTGGTTCTCCCTCAAGTGTTTTTTACACTGGGTGTTCAAATTTATTTTAAGAGATAGGGAGGGAGGGGACATAAAGGGAATGCATACATTGCTAGTCACATTTTTAAAATTAACATTTTGGAATAGTGTTTATGGAAATCTTTAGCTTTTAATCATTTTTACCAATTTGAACAGTTTAATAAACTGGTTCTGCTCTCTTCTGAATCTCATGCCTTTGGCACCTTGGTAGGTGCAGGAGGAGCTCAGTGCAAAAATCACTTTGGGGCCTCATTAACCCTTTAGAGACAAGCTTTGCCCCAGGCTGCGGACCAGACAGATGCTTAGGGAAGGTTGATACCAGCTTCAGTCTCTACTGGATTAGCCCTACTCTTTCCTTTCCCCTCCATTATTTAGTGACTCTGTAAGTAAGTTAAATACACCCTTATTATTTAGCTGTTAAGTAACTATAATGAAATCTGCTGCAAAATCTCTCTTGGAATCCATGTGCCCAGGATTATATTAGCATTATTTTTAATAAATCTATATGCTTAAAAAAAAAAAAAAAAAAA
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 16299, GC content failed 697, low tm 6376, high tm 5935, high any compl 2, high end compl 43, high repeat similarity 73, long poly-x seq 21, ok 3152
PRIMER_RIGHT_EXPLAIN=considered 16299, GC content failed 1010, low tm 6416, high tm 5595, high end compl 10, high repeat similarity 23, long poly-x seq 21, ok 3224
PRIMER_PAIR_EXPLAIN=considered 623, unacceptable product size 565, high end compl 1, ok 57
PRIMER_PAIR_PENALTY=0.0425
PRIMER_LEFT_PENALTY=0.011619
PRIMER_RIGHT_PENALTY=0.030877
PRIMER_LEFT_SEQUENCE=TCTCTTCTCAAATGGCTGACTTC
PRIMER_RIGHT_SEQUENCE=AGCTGGTATCAACCTTCCCTAAG
PRIMER_LEFT=901,23
PRIMER_RIGHT=1264,23
PRIMER_LEFT_TM=60.012
PRIMER_RIGHT_TM=60.031
PRIMER_LEFT_GC_PERCENT=43.478
PRIMER_RIGHT_GC_PERCENT=47.826
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_MISPRIMING_SCORE=12.00, MER43 Homo sapiens clone DOE1-35 MER repetitive sequence - a consensus
PRIMER_PAIR_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=6.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=364
PRIMER_PAIR_PENALTY_1=0.1252
PRIMER_LEFT_1_PENALTY=0.094334
PRIMER_RIGHT_1_PENALTY=0.030877
PRIMER_LEFT_1_SEQUENCE=CAGACACCTTATGGCATAGTTCC
PRIMER_RIGHT_1_SEQUENCE=AGCTGGTATCAACCTTCCCTAAG
PRIMER_LEFT_1=755,23
PRIMER_RIGHT_1=1264,23
PRIMER_LEFT_1_TM=59.906
PRIMER_RIGHT_1_TM=60.031
PRIMER_LEFT_1_GC_PERCENT=47.826
PRIMER_RIGHT_1_GC_PERCENT=47.826
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, MER43 Homo sapiens clone DOE1-35 MER repetitive sequence - a consensus
PRIMER_PAIR_1_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=6.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=510
PRIMER_PAIR_PENALTY_2=0.2658
PRIMER_LEFT_2_PENALTY=0.011619
PRIMER_RIGHT_2_PENALTY=0.254201
PRIMER_LEFT_2_SEQUENCE=TCTCTTCTCAAATGGCTGACTTC
PRIMER_RIGHT_2_SEQUENCE=TGCTAATATAATCCTGGGCACAT
PRIMER_LEFT_2=901,23
PRIMER_RIGHT_2=1426,23
PRIMER_LEFT_2_TM=60.012
PRIMER_RIGHT_2_TM=59.746
PRIMER_LEFT_2_GC_PERCENT=43.478
PRIMER_RIGHT_2_GC_PERCENT=39.130
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_2_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_2_MISPRIMING_SCORE=22.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=526
PRIMER_PAIR_PENALTY_3=0.3083
PRIMER_LEFT_3_PENALTY=0.277414
PRIMER_RIGHT_3_PENALTY=0.030877
PRIMER_LEFT_3_SEQUENCE=GTGTATCGCGTACCTGATGAAAT
PRIMER_RIGHT_3_SEQUENCE=AGCTGGTATCAACCTTCCCTAAG
PRIMER_LEFT_3=595,23
PRIMER_RIGHT_3=1264,23
PRIMER_LEFT_3_TM=60.277
PRIMER_RIGHT_3_TM=60.031
PRIMER_LEFT_3_GC_PERCENT=43.478
PRIMER_RIGHT_3_GC_PERCENT=47.826
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, MER43 Homo sapiens clone DOE1-35 MER repetitive sequence - a consensus
PRIMER_PAIR_3_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=6.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=670
PRIMER_PAIR_PENALTY_4=0.3212
PRIMER_LEFT_4_PENALTY=0.011619
PRIMER_RIGHT_4_PENALTY=0.309542
PRIMER_LEFT_4_SEQUENCE=TCTCTTCTCAAATGGCTGACTTC
PRIMER_RIGHT_4_SEQUENCE=TCAGAAGAGAGCAGAACCAGTTT
PRIMER_LEFT_4=901,23
PRIMER_RIGHT_4=1125,23
PRIMER_LEFT_4_TM=60.012
PRIMER_RIGHT_4_TM=59.690
PRIMER_LEFT_4_GC_PERCENT=43.478
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_4_MISPRIMING_SCORE=24.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=8.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=225
PRIMER_PAIR_PENALTY_5=0.3485
PRIMER_LEFT_5_PENALTY=0.094334
PRIMER_RIGHT_5_PENALTY=0.254201
PRIMER_LEFT_5_SEQUENCE=CAGACACCTTATGGCATAGTTCC
PRIMER_RIGHT_5_SEQUENCE=TGCTAATATAATCCTGGGCACAT
PRIMER_LEFT_5=755,23
PRIMER_RIGHT_5=1426,23
PRIMER_LEFT_5_TM=59.906
PRIMER_RIGHT_5_TM=59.746
PRIMER_LEFT_5_GC_PERCENT=47.826
PRIMER_RIGHT_5_GC_PERCENT=39.130
PRIMER_LEFT_5_SELF_ANY=7.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=2.00
PRIMER_RIGHT_5_SELF_END=2.00
PRIMER_LEFT_5_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_5_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_5_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_5_END_STABILITY=7.9000
PRIMER_RIGHT_5_END_STABILITY=6.6000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_5=672
PRIMER_PAIR_PENALTY_6=0.3691
PRIMER_LEFT_6_PENALTY=0.011619
PRIMER_RIGHT_6_PENALTY=0.357451
PRIMER_LEFT_6_SEQUENCE=TCTCTTCTCAAATGGCTGACTTC
PRIMER_RIGHT_6_SEQUENCE=AAGCTGGTATCAACCTTCCCTAA
PRIMER_LEFT_6=901,23
PRIMER_RIGHT_6=1265,23
PRIMER_LEFT_6_TM=60.012
PRIMER_RIGHT_6_TM=60.357
PRIMER_LEFT_6_GC_PERCENT=43.478
PRIMER_RIGHT_6_GC_PERCENT=43.478
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=2.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_6_MISPRIMING_SCORE=11.00, MER4C a consensus
PRIMER_PAIR_6_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_6_END_STABILITY=6.4000
PRIMER_RIGHT_6_END_STABILITY=7.5000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_6=365
PRIMER_PAIR_PENALTY_7=0.3717
PRIMER_LEFT_7_PENALTY=0.277414
PRIMER_RIGHT_7_PENALTY=0.094334
PRIMER_LEFT_7_SEQUENCE=GTGTATCGCGTACCTGATGAAAT
PRIMER_RIGHT_7_SEQUENCE=GGAACTATGCCATAAGGTGTCTG
PRIMER_LEFT_7=595,23
PRIMER_RIGHT_7=777,23
PRIMER_LEFT_7_TM=60.277
PRIMER_RIGHT_7_TM=59.906
PRIMER_LEFT_7_GC_PERCENT=43.478
PRIMER_RIGHT_7_GC_PERCENT=47.826
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=7.00
PRIMER_LEFT_7_SELF_END=2.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_7_MISPRIMING_SCORE=12.00, PTR5 Human PTR5 mRNA for repetitive sequence
PRIMER_PAIR_7_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_7_END_STABILITY=6.9000
PRIMER_RIGHT_7_END_STABILITY=6.4000
PRIMER_PAIR_7_COMPL_ANY=6.00
PRIMER_PAIR_7_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_7=183
PRIMER_PAIR_PENALTY_8=0.3735
PRIMER_LEFT_8_PENALTY=0.011619
PRIMER_RIGHT_8_PENALTY=0.361920
PRIMER_LEFT_8_SEQUENCE=TCTCTTCTCAAATGGCTGACTTC
PRIMER_RIGHT_8_SEQUENCE=TATCAACCTTCCCTAAGCATCTG
PRIMER_LEFT_8=901,23
PRIMER_RIGHT_8=1258,23
PRIMER_LEFT_8_TM=60.012
PRIMER_RIGHT_8_TM=59.638
PRIMER_LEFT_8_GC_PERCENT=43.478
PRIMER_RIGHT_8_GC_PERCENT=43.478
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=2.00
PRIMER_RIGHT_8_SELF_END=2.00
PRIMER_LEFT_8_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_8_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_8_END_STABILITY=6.4000
PRIMER_RIGHT_8_END_STABILITY=6.6000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_8=358
PRIMER_PAIR_PENALTY_9=0.3851
PRIMER_LEFT_9_PENALTY=0.011619
PRIMER_RIGHT_9_PENALTY=0.373459
PRIMER_LEFT_9_SEQUENCE=TCTCTTCTCAAATGGCTGACTTC
PRIMER_RIGHT_9_SEQUENCE=ATCAACCTTCCCTAAGCATCTGT
PRIMER_LEFT_9=901,23
PRIMER_RIGHT_9=1257,23
PRIMER_LEFT_9_TM=60.012
PRIMER_RIGHT_9_TM=60.373
PRIMER_LEFT_9_GC_PERCENT=43.478
PRIMER_RIGHT_9_GC_PERCENT=43.478
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=2.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_9_MISPRIMING_SCORE=11.00, LTR5 LTR from human endogenous retrovirus 5' LTR, clone HERV-K18
PRIMER_PAIR_9_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_9_END_STABILITY=6.4000
PRIMER_RIGHT_9_END_STABILITY=6.4000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_9=357
PRIMER_PAIR_PENALTY_10=0.4039
PRIMER_LEFT_10_PENALTY=0.094334
PRIMER_RIGHT_10_PENALTY=0.309542
PRIMER_LEFT_10_SEQUENCE=CAGACACCTTATGGCATAGTTCC
PRIMER_RIGHT_10_SEQUENCE=TCAGAAGAGAGCAGAACCAGTTT
PRIMER_LEFT_10=755,23
PRIMER_RIGHT_10=1125,23
PRIMER_LEFT_10_TM=59.906
PRIMER_RIGHT_10_TM=59.690
PRIMER_LEFT_10_GC_PERCENT=47.826
PRIMER_RIGHT_10_GC_PERCENT=43.478
PRIMER_LEFT_10_SELF_ANY=7.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=2.00
PRIMER_RIGHT_10_SELF_END=3.00
PRIMER_LEFT_10_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_10_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_10_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_10_END_STABILITY=7.9000
PRIMER_RIGHT_10_END_STABILITY=6.7000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_10=371
PRIMER_PAIR_PENALTY_11=0.4518
PRIMER_LEFT_11_PENALTY=0.094334
PRIMER_RIGHT_11_PENALTY=0.357451
PRIMER_LEFT_11_SEQUENCE=CAGACACCTTATGGCATAGTTCC
PRIMER_RIGHT_11_SEQUENCE=AAGCTGGTATCAACCTTCCCTAA
PRIMER_LEFT_11=755,23
PRIMER_RIGHT_11=1265,23
PRIMER_LEFT_11_TM=59.906
PRIMER_RIGHT_11_TM=60.357
PRIMER_LEFT_11_GC_PERCENT=47.826
PRIMER_RIGHT_11_GC_PERCENT=43.478
PRIMER_LEFT_11_SELF_ANY=7.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=2.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_11_MISPRIMING_SCORE=11.00, MER4C a consensus
PRIMER_PAIR_11_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_11_END_STABILITY=7.9000
PRIMER_RIGHT_11_END_STABILITY=7.5000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_11=511
PRIMER_PAIR_PENALTY_12=0.4563
PRIMER_LEFT_12_PENALTY=0.094334
PRIMER_RIGHT_12_PENALTY=0.361920
PRIMER_LEFT_12_SEQUENCE=CAGACACCTTATGGCATAGTTCC
PRIMER_RIGHT_12_SEQUENCE=TATCAACCTTCCCTAAGCATCTG
PRIMER_LEFT_12=755,23
PRIMER_RIGHT_12=1258,23
PRIMER_LEFT_12_TM=59.906
PRIMER_RIGHT_12_TM=59.638
PRIMER_LEFT_12_GC_PERCENT=47.826
PRIMER_RIGHT_12_GC_PERCENT=43.478
PRIMER_LEFT_12_SELF_ANY=7.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=2.00
PRIMER_RIGHT_12_SELF_END=2.00
PRIMER_LEFT_12_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_12_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_12_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_12_END_STABILITY=7.9000
PRIMER_RIGHT_12_END_STABILITY=6.6000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_12=504
PRIMER_PAIR_PENALTY_13=0.4585
PRIMER_LEFT_13_PENALTY=0.011619
PRIMER_RIGHT_13_PENALTY=0.446835
PRIMER_LEFT_13_SEQUENCE=TCTCTTCTCAAATGGCTGACTTC
PRIMER_RIGHT_13_SEQUENCE=CTAGCAATGTATGCATTCCCTTT
PRIMER_LEFT_13=901,23
PRIMER_RIGHT_13=1017,23
PRIMER_LEFT_13_TM=60.012
PRIMER_RIGHT_13_TM=59.553
PRIMER_LEFT_13_GC_PERCENT=43.478
PRIMER_RIGHT_13_GC_PERCENT=39.130
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=8.00
PRIMER_LEFT_13_SELF_END=2.00
PRIMER_RIGHT_13_SELF_END=2.00
PRIMER_LEFT_13_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_13_MISPRIMING_SCORE=12.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_13_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_13_END_STABILITY=6.4000
PRIMER_RIGHT_13_END_STABILITY=8.5000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_13=117
PRIMER_PAIR_PENALTY_14=0.4678
PRIMER_LEFT_14_PENALTY=0.094334
PRIMER_RIGHT_14_PENALTY=0.373459
PRIMER_LEFT_14_SEQUENCE=CAGACACCTTATGGCATAGTTCC
PRIMER_RIGHT_14_SEQUENCE=ATCAACCTTCCCTAAGCATCTGT
PRIMER_LEFT_14=755,23
PRIMER_RIGHT_14=1257,23
PRIMER_LEFT_14_TM=59.906
PRIMER_RIGHT_14_TM=60.373
PRIMER_LEFT_14_GC_PERCENT=47.826
PRIMER_RIGHT_14_GC_PERCENT=43.478
PRIMER_LEFT_14_SELF_ANY=7.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=2.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_14_MISPRIMING_SCORE=11.00, LTR5 LTR from human endogenous retrovirus 5' LTR, clone HERV-K18
PRIMER_PAIR_14_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_14_END_STABILITY=7.9000
PRIMER_RIGHT_14_END_STABILITY=6.4000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_14=503
PRIMER_PAIR_PENALTY_15=0.4863
PRIMER_LEFT_15_PENALTY=0.277414
PRIMER_RIGHT_15_PENALTY=0.208920
PRIMER_LEFT_15_SEQUENCE=GTGTATCGCGTACCTGATGAAAT
PRIMER_RIGHT_15_SEQUENCE=CTTCTCATAGACGTCGGGAACTA
PRIMER_LEFT_15=595,23
PRIMER_RIGHT_15=793,23
PRIMER_LEFT_15_TM=60.277
PRIMER_RIGHT_15_TM=59.791
PRIMER_LEFT_15_GC_PERCENT=43.478
PRIMER_RIGHT_15_GC_PERCENT=47.826
PRIMER_LEFT_15_SELF_ANY=6.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=2.00
PRIMER_RIGHT_15_SELF_END=2.00
PRIMER_LEFT_15_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_15_MISPRIMING_SCORE=12.00, MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_PAIR_15_MISPRIMING_SCORE=24.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_15_END_STABILITY=6.9000
PRIMER_RIGHT_15_END_STABILITY=5.7000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_15=199
PRIMER_PAIR_PENALTY_16=0.4902
PRIMER_LEFT_16_PENALTY=0.459340
PRIMER_RIGHT_16_PENALTY=0.030877
PRIMER_LEFT_16_SEQUENCE=AGGGAATGCATACATTGCTAGTC
PRIMER_RIGHT_16_SEQUENCE=AGCTGGTATCAACCTTCCCTAAG
PRIMER_LEFT_16=997,23
PRIMER_RIGHT_16=1264,23
PRIMER_LEFT_16_TM=59.541
PRIMER_RIGHT_16_TM=60.031
PRIMER_LEFT_16_GC_PERCENT=43.478
PRIMER_RIGHT_16_GC_PERCENT=47.826
PRIMER_LEFT_16_SELF_ANY=8.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=2.00
PRIMER_RIGHT_16_SELF_END=3.00
PRIMER_LEFT_16_MISPRIMING_SCORE=11.00, L1MB7 3'-end of L1 repeat (subfamily L1MB7) - a consensus sequence
PRIMER_RIGHT_16_MISPRIMING_SCORE=12.00, MER43 Homo sapiens clone DOE1-35 MER repetitive sequence - a consensus
PRIMER_PAIR_16_MISPRIMING_SCORE=20.00, MER4C a consensus
PRIMER_LEFT_16_END_STABILITY=5.4000
PRIMER_RIGHT_16_END_STABILITY=6.0000
PRIMER_PAIR_16_COMPL_ANY=6.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_16=268
PRIMER_PAIR_PENALTY_17=0.5201
PRIMER_LEFT_17_PENALTY=0.489183
PRIMER_RIGHT_17_PENALTY=0.030877
PRIMER_LEFT_17_SEQUENCE=CTCTCCACTCTCTTCTCAAATGG
PRIMER_RIGHT_17_SEQUENCE=AGCTGGTATCAACCTTCCCTAAG
PRIMER_LEFT_17=893,23
PRIMER_RIGHT_17=1264,23
PRIMER_LEFT_17_TM=59.511
PRIMER_RIGHT_17_TM=60.031
PRIMER_LEFT_17_GC_PERCENT=47.826
PRIMER_RIGHT_17_GC_PERCENT=47.826
PRIMER_LEFT_17_SELF_ANY=3.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=3.00
PRIMER_LEFT_17_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_17_MISPRIMING_SCORE=12.00, MER43 Homo sapiens clone DOE1-35 MER repetitive sequence - a consensus
PRIMER_PAIR_17_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_17_END_STABILITY=8.4000
PRIMER_RIGHT_17_END_STABILITY=6.0000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_17=372
PRIMER_PAIR_PENALTY_18=0.5316
PRIMER_LEFT_18_PENALTY=0.277414
PRIMER_RIGHT_18_PENALTY=0.254201
PRIMER_LEFT_18_SEQUENCE=GTGTATCGCGTACCTGATGAAAT
PRIMER_RIGHT_18_SEQUENCE=TGCTAATATAATCCTGGGCACAT
PRIMER_LEFT_18=595,23
PRIMER_RIGHT_18=1426,23
PRIMER_LEFT_18_TM=60.277
PRIMER_RIGHT_18_TM=59.746
PRIMER_LEFT_18_GC_PERCENT=43.478
PRIMER_RIGHT_18_GC_PERCENT=39.130
PRIMER_LEFT_18_SELF_ANY=6.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=2.00
PRIMER_RIGHT_18_SELF_END=2.00
PRIMER_LEFT_18_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_18_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_18_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_18_END_STABILITY=6.9000
PRIMER_RIGHT_18_END_STABILITY=6.6000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_18=832
PRIMER_PAIR_PENALTY_19=0.5412
PRIMER_LEFT_19_PENALTY=0.094334
PRIMER_RIGHT_19_PENALTY=0.446835
PRIMER_LEFT_19_SEQUENCE=CAGACACCTTATGGCATAGTTCC
PRIMER_RIGHT_19_SEQUENCE=CTAGCAATGTATGCATTCCCTTT
PRIMER_LEFT_19=755,23
PRIMER_RIGHT_19=1017,23
PRIMER_LEFT_19_TM=59.906
PRIMER_RIGHT_19_TM=59.553
PRIMER_LEFT_19_GC_PERCENT=47.826
PRIMER_RIGHT_19_GC_PERCENT=39.130
PRIMER_LEFT_19_SELF_ANY=7.00
PRIMER_RIGHT_19_SELF_ANY=8.00
PRIMER_LEFT_19_SELF_END=2.00
PRIMER_RIGHT_19_SELF_END=2.00
PRIMER_LEFT_19_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_19_MISPRIMING_SCORE=12.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_19_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_19_END_STABILITY=7.9000
PRIMER_RIGHT_19_END_STABILITY=8.5000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_19=263
=