PRIMER_SEQUENCE_ID=NM_007039
SEQUENCE=GCCCATGAGCGCGCCGCGGCCCGGGCTGGCGTGCGGGTGCGGCTGCGGCGGCCGCGCGGCGGGGCCCCGGGAGGCGGGTCGCTGAGCGGGGCGCGCGGCCCCGAGGATGCGGGAGCGGGAGCGGGAGCAGCGCTGGCGTCAATGCTCCCTTCCTCGGGCCATTGGAGACTCCGTTGCTTTTTAATGGCGGCAGCGGCTGCTGGGTGAGCAGCTGGAGGCCGGACAGTGTTCGTCCCATCCGGAGAGGATCGCTTTCTCCTGGCGTCACCAGCGCTGGGTTGGTGGGGGTAGCTTTTCCCTCTTTGCTCCTCCATTCTTGAAGAAAGAAGAAGATGCCACTGCCATTTGGGTTGAAACTGAAACGCACCCGGCGCTACACGGTGTCCAGCAAGAGTTGCCTGGTTGCCCGGATCCAACTGCTTAATAACGAGTTTGTGGAGTTCACCCTGTCCGTGGAGAGCACTGGCCAGGAAAGCCTCGAGGCCGTGGCCCAGAGGCTGGAGCTGCGGGAGGTCACTTACTTCAGCCTCTGGTACTACAACAAGCAAAATCAGCGCCGGTGGGTAGATTTGGAAAAACCTTTGAAGAAGCAGCTGGATAAATATGCATTGGAACCTACCGTCTATTTTGGAGTGGTGTTTTATGTGCCTTCAGTTTCTCAGCTGCAGCAGGAGATTACCAGGTATCAGTATTATCTGCAACTGAAGAAAGATATCTTGGAAGGAAGTATTCCTTGTACCTTAGAACAAGCAATTCAGCTAGCAGGCTTAGCTGTTCAAGCGGATTTTGGTGACTTTGATCAGTATGAATCCCAGGACTTTCTTCAGAAATTTGCCTTGTTTCCTGTGGGATGGTTACAAGATGAAAAAGTATTGGAAGAAGCAACCCAAAAAGTGGCCTTACTACATCAGAAATACAGAGGGCTCACAGCTCCTGATGCTGAAATGCTGTACATGCAGGAGGTAGAGAGAATGGATGGCTATGGAGAAGAGAGCTACCCTGCTAAGGATAGCCAAGGAAGTGACATATCCATTGGAGCGTGTCTTGAAGGTATCTTTGTGAAACACAAGAATGGAAGGCATCCTGTGGTATTTAGGTGGCATGACATTGCCAACATGTCCCACAACAAGTCCTTTTTTGCATTAGAGCTGGCAAATAAAGAGGAGACCATTCAATTTCAAACTGAAGACATGGAAACAGCAAAATACATTTGGAGACTCTGTGTTGCGCGACACAAGTTTTACAGACTAAACCAGTGTAACCTGCAAACTCAGACTGTCACAGTGAACCCAATCAGGAGGAGGTCTTCTTCAAGGATGTCTCTGCCTAAACCCCAGCCCTACGTGATGCCTCCCCCACCGCAGTTGCACTATAATGGACATTATACAGAACCATATGCTTCTTCCCAAGATAACCTCTTTGTGCCCAACCAGAACGGATACTACTGTCACTCTCAGACAAGCTTGGATAGAGCCCAGATTGACTTCAACGGTCGGATCCGTAATGGCAGTGTCTACAGTGCACACAGCACCAACTCCTTAAATAATCCTCAGCCCTACTTGCAGCCCTCGCCGATGTCGTCCAACCCTAGCATCACCGGGAGTGACGTCATGAGGCCTGACTACCTCCCGTCCCATCGGCACAGCGCCGTGATACCCCCGTCCTACCGCCCCACCCCAGACTATGAGACTGTGATGAAGCAGCTCAACAGGGGCCTGGTGCATGCGGAACGGCAGAGCCACTCGCTGCGAAACCTCAACATCGGCAGCTCGTACGCCTACAGCAGGCCCGCGGCGCTGGTCTACAGCCAGCCCGAGATCCGCGAGCACGCACAGCTCCCCTCGCCAGCGGCCGCACACTGCCCGTTCAGCCTGAGCTACAGCTTCCACAGCCCGTCTCCCTACCCCTACCCTGCCGAGCGGCGGCCCGTGGTGGGCGCGGTCAGCGTGCCGGAGCTGACCAATGCGCAGCTGCAGGCGCAGGACTACCCGTCTCCCAACATCATGCGGACGCAGGTGTACCGGCCACCCCCACCCTACCCGCCCCCCAGGCCCGCCAACAGCACGCCAGACCTGTCCCGCCACCTTTACATCAGCAGCAGCAACCCCGACCTCATCACGCGGCGCGTGCACCACTCGGTGCAAACGTTCCAGGAGGACAGCCTGCCCGTGGCGCACTCGCTGCAGGAGGTCAGCGAGCCCCTCACCGCCGCGCGCCACGCGCAGCTGCACAAACGGAACAGCATCGAGGTGGCCGGGCTCAGCCACGGCCTGGAGGGCCTGCGGCTCAAGGAGCGCACCCTATCCGCGTCGGCGGCAGAGGTGGCGCCGCGAGCCGTCTCGGTGGGCTCCCAGCCCAGCGTTTTCACCGAGAGGACACAGCGAGAAGGGCCGGAGGAGGCGGAGGGCTTGAGGTACGGCCATAAGAAGTCCCTGTCGGACGCCACCATGCTAATCCACAGCAGCGAGGAGGAGGAGGACGAGGACTTCGAGGAGGAGAGCGGGGCCCGGGCGCCCCCTGCACGTGCGCGCGAGCCTCGGCCCGGCCTGGCCCAGGACCCACCTGGCTGCCCTCGCGTCCTGCTCGCCGGGCCCCTGCACATCCTGGAGCCCAAGGCCCACGTCCCAGACGCGGAGAAGAGGATGATGGACAGCAGCCCCGTCCGCACGACCGCAGAGGCCCAGCGGCCCTGGAGAGACGGGCTGCTGATGCCCTCCATGTCGGAGTCCGACCTCACCACGTCAGGCCGCTACCGAGCCCGGAGGGACTCTCTGAAGAAAAGGCCGGTGTCGGACCTTCTCTCTGGGAAGAAGAACATCGTGGAAGGGCTCCCGCCTCTAGGGGGAATGAAAAAGACTCGAGTAGATGCAAAAAAAATTGGTCCTCTTAAACTGGCTGCCCTAAATGGACTCTCCCTATCTCGAGTGCCTCTGCCTGATGAAGGAAAGGAAGTGGCTACCAGAGCAACGAATGATGAAAGGTGTAAAATTCTGGAACAACGATTAGAACAAGGAATGGTATTCACAGAATATGAAAGAATTCTTAAGAAACGGCTAGTTGATGGGGAGTGCTCAACAGCACGACTCCCTGAAAATGCAGAAAGAAATCGATTCCAAGATGTTCTTCCTTATGATGATGCGAGAGTGGAGTTGGTCCCAACTAAAGAAAACAACACTGGTTACATCAACGCATCACATATTAAGGTCTCTGTCAGTGGAATCGAATGGGATTATATTGCCACACAGGGACCATTACAGAATACCTGTCAAGATTTTTGGCAGATGGTATGGGAACAGGGAATTGCAATTATAGCAATGGTGACAGCAGAAGAGGAGGGTGGAAGGGAGAAGAGCTTTAGGTACTGGCCACGACTTGGTTCCAGGCACAACACTGTCACCTATGGAAGGTTTAAGATCACGACCCGGTTCCGCACAGACTCTGGCTGCTATGCCACCACAGGCCTGAAGATGAAGCACCTCCTTACCGGGCAAGAGAGGACCGTCTGGCACCTCCAATACACAGACTGGCCTGAACATGGCTGTCCAGAAGACCTCAAGGGATTTTTATCATATCTTGAAGAGATCCAGTCTGTTCGACGCCATACAAATAGCACAAGTGATCCCCAAAGCCCCAACCCTCCGTTGTTGGTCCACTGCAGTGCTGGGGTAGGAAGGACTGGCGTGGTGATTTTGTCGGAGATCATGATCGCCTGCCTGGAACACAATGAGGTGCTGGACATCCCGAGAGTGCTGGACATGCTGAGGCAACAGAGAATGATGCTGGTGCAGACTCTCTGCCAGTACACATTTGTGTACAGAGTCCTCATCCAGTTCCTGAAAAGCTCCAGGCTCATCTAAGCTCCCACAATTTCTTACGGGGCCAGTCATGTGAAGCGTTTACAGCTTAAAAAAAAAGCGCTTGCCTAACTCATACTTTCCCGTTGACACTTGATCCACGCAGCGTGGCACTGGGACGTAAGTGGCGCAGTCTGAATGGCGGCACGCTGAAGGAAACGTGCGAAGCACAGGCTGAAGAGGGGTTTCTAACCTGGGAAAGGTGCTCAAGGAGGACTTGGTTTCAAGGGCCTTGCCCTGCCCCGGTCTGTCCGGTTTGCAGTACTTGCACACATTTTGGAGATTGTATTTTCTAGACAATTCTCTATTTTACTGAAGCTATGCTGGGCAATTCTGGCAATCATTAAAGTGCATAGATTTCTATCTTAAAAAAAAAAAAA
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 49003, GC content failed 3057, low tm 15466, high tm 20681, high end compl 16, high repeat similarity 22, long poly-x seq 80, ok 9681
PRIMER_RIGHT_EXPLAIN=considered 48981, GC content failed 2246, low tm 16059, high tm 20758, high end compl 4, high repeat similarity 6, long poly-x seq 87, ok 9821
PRIMER_PAIR_EXPLAIN=considered 400, unacceptable product size 359, ok 41
PRIMER_PAIR_PENALTY=0.0369
PRIMER_LEFT_PENALTY=0.030116
PRIMER_RIGHT_PENALTY=0.006805
PRIMER_LEFT_SEQUENCE=CTTTGAAGAAGCAGCTGGATAAA
PRIMER_RIGHT_SEQUENCE=GCAAGTAGGGCTGAGGATTATTT
PRIMER_LEFT=579,23
PRIMER_RIGHT=1562,23
PRIMER_LEFT_TM=60.030
PRIMER_RIGHT_TM=60.007
PRIMER_LEFT_GC_PERCENT=39.130
PRIMER_RIGHT_GC_PERCENT=43.478
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_MISPRIMING_SCORE=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_MISPRIMING_SCORE=11.00, reverse MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_PAIR_MISPRIMING_SCORE=21.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_LEFT_END_STABILITY=6.2000
PRIMER_RIGHT_END_STABILITY=6.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=984
PRIMER_PAIR_PENALTY_1=0.0383
PRIMER_LEFT_1_PENALTY=0.030116
PRIMER_RIGHT_1_PENALTY=0.008181
PRIMER_LEFT_1_SEQUENCE=CTTTGAAGAAGCAGCTGGATAAA
PRIMER_RIGHT_1_SEQUENCE=AAATTTCTGAAGAAAGTCCTGGG
PRIMER_LEFT_1=579,23
PRIMER_RIGHT_1=832,23
PRIMER_LEFT_1_TM=60.030
PRIMER_RIGHT_1_TM=59.992
PRIMER_LEFT_1_GC_PERCENT=39.130
PRIMER_RIGHT_1_GC_PERCENT=39.130
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_MISPRIMING_SCORE=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_1_MISPRIMING_SCORE=23.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_LEFT_1_END_STABILITY=6.2000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=254
PRIMER_PAIR_PENALTY_2=0.0545
PRIMER_LEFT_2_PENALTY=0.047652
PRIMER_RIGHT_2_PENALTY=0.006805
PRIMER_LEFT_2_SEQUENCE=TATGAATCCCAGGACTTTCTTCA
PRIMER_RIGHT_2_SEQUENCE=GCAAGTAGGGCTGAGGATTATTT
PRIMER_LEFT_2=803,23
PRIMER_RIGHT_2=1562,23
PRIMER_LEFT_2_TM=59.952
PRIMER_RIGHT_2_TM=60.007
PRIMER_LEFT_2_GC_PERCENT=39.130
PRIMER_RIGHT_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_2_MISPRIMING_SCORE=11.00, reverse MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_PAIR_2_MISPRIMING_SCORE=21.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.2000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=760
PRIMER_PAIR_PENALTY_3=0.0722
PRIMER_LEFT_3_PENALTY=0.065377
PRIMER_RIGHT_3_PENALTY=0.006805
PRIMER_LEFT_3_SEQUENCE=CTACCGTCTATTTTGGAGTGGTG
PRIMER_RIGHT_3_SEQUENCE=GCAAGTAGGGCTGAGGATTATTT
PRIMER_LEFT_3=615,23
PRIMER_RIGHT_3=1562,23
PRIMER_LEFT_3_TM=59.935
PRIMER_RIGHT_3_TM=60.007
PRIMER_LEFT_3_GC_PERCENT=47.826
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_MISPRIMING_SCORE=11.00, reverse L1PB3 3'-end of L1 repeat (subfamily L1PB3) - a consensus sequence
PRIMER_RIGHT_3_MISPRIMING_SCORE=11.00, reverse MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_PAIR_3_MISPRIMING_SCORE=19.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=948
PRIMER_PAIR_PENALTY_4=0.0736
PRIMER_LEFT_4_PENALTY=0.065377
PRIMER_RIGHT_4_PENALTY=0.008181
PRIMER_LEFT_4_SEQUENCE=CTACCGTCTATTTTGGAGTGGTG
PRIMER_RIGHT_4_SEQUENCE=AAATTTCTGAAGAAAGTCCTGGG
PRIMER_LEFT_4=615,23
PRIMER_RIGHT_4=832,23
PRIMER_LEFT_4_TM=59.935
PRIMER_RIGHT_4_TM=59.992
PRIMER_LEFT_4_GC_PERCENT=47.826
PRIMER_RIGHT_4_GC_PERCENT=39.130
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_MISPRIMING_SCORE=11.00, reverse L1PB3 3'-end of L1 repeat (subfamily L1PB3) - a consensus sequence
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_4_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=218
PRIMER_PAIR_PENALTY_5=0.0750
PRIMER_LEFT_5_PENALTY=0.068149
PRIMER_RIGHT_5_PENALTY=0.006805
PRIMER_LEFT_5_SEQUENCE=TAGAGAGAATGGATGGCTATGGA
PRIMER_RIGHT_5_SEQUENCE=GCAAGTAGGGCTGAGGATTATTT
PRIMER_LEFT_5=963,23
PRIMER_RIGHT_5=1562,23
PRIMER_LEFT_5_TM=60.068
PRIMER_RIGHT_5_TM=60.007
PRIMER_LEFT_5_GC_PERCENT=43.478
PRIMER_RIGHT_5_GC_PERCENT=43.478
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=2.00
PRIMER_RIGHT_5_SELF_END=3.00
PRIMER_LEFT_5_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_5_MISPRIMING_SCORE=11.00, reverse MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_PAIR_5_MISPRIMING_SCORE=20.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_5_END_STABILITY=8.1000
PRIMER_RIGHT_5_END_STABILITY=6.2000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_5=600
PRIMER_PAIR_PENALTY_6=0.0778
PRIMER_LEFT_6_PENALTY=0.030116
PRIMER_RIGHT_6_PENALTY=0.047652
PRIMER_LEFT_6_SEQUENCE=CTTTGAAGAAGCAGCTGGATAAA
PRIMER_RIGHT_6_SEQUENCE=TGAAGAAAGTCCTGGGATTCATA
PRIMER_LEFT_6=579,23
PRIMER_RIGHT_6=825,23
PRIMER_LEFT_6_TM=60.030
PRIMER_RIGHT_6_TM=59.952
PRIMER_LEFT_6_GC_PERCENT=39.130
PRIMER_RIGHT_6_GC_PERCENT=39.130
PRIMER_LEFT_6_SELF_ANY=6.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=3.00
PRIMER_RIGHT_6_SELF_END=2.00
PRIMER_LEFT_6_MISPRIMING_SCORE=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_6_MISPRIMING_SCORE=12.00, MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_PAIR_6_MISPRIMING_SCORE=23.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_LEFT_6_END_STABILITY=6.2000
PRIMER_RIGHT_6_END_STABILITY=5.9000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_6=247
PRIMER_PAIR_PENALTY_7=0.0834
PRIMER_LEFT_7_PENALTY=0.016975
PRIMER_RIGHT_7_PENALTY=0.066430
PRIMER_LEFT_7_SEQUENCE=AATGGGATTATATTGCCACACAG
PRIMER_RIGHT_7_SEQUENCE=GCCAGTACCTAAAGCTCTTCTCC
PRIMER_LEFT_7=3222,23
PRIMER_RIGHT_7=3365,23
PRIMER_LEFT_7_TM=59.983
PRIMER_RIGHT_7_TM=59.934
PRIMER_LEFT_7_GC_PERCENT=39.130
PRIMER_RIGHT_7_GC_PERCENT=52.174
PRIMER_LEFT_7_SELF_ANY=8.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_7_MISPRIMING_SCORE=11.00, THE1BR THE-1B Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_PAIR_7_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_7_END_STABILITY=6.7000
PRIMER_RIGHT_7_END_STABILITY=7.9000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_7=144
PRIMER_PAIR_PENALTY_8=0.0834
PRIMER_LEFT_8_PENALTY=0.016975
PRIMER_RIGHT_8_PENALTY=0.066430
PRIMER_LEFT_8_SEQUENCE=AATGGGATTATATTGCCACACAG
PRIMER_RIGHT_8_SEQUENCE=GGCCAGTACCTAAAGCTCTTCTC
PRIMER_LEFT_8=3222,23
PRIMER_RIGHT_8=3366,23
PRIMER_LEFT_8_TM=59.983
PRIMER_RIGHT_8_TM=59.934
PRIMER_LEFT_8_GC_PERCENT=39.130
PRIMER_RIGHT_8_GC_PERCENT=52.174
PRIMER_LEFT_8_SELF_ANY=8.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, THE1BR THE-1B Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_PAIR_8_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_8_END_STABILITY=6.7000
PRIMER_RIGHT_8_END_STABILITY=6.7000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_8=145
PRIMER_PAIR_PENALTY_9=0.0931
PRIMER_LEFT_9_PENALTY=0.030116
PRIMER_RIGHT_9_PENALTY=0.063026
PRIMER_LEFT_9_SEQUENCE=CTTTGAAGAAGCAGCTGGATAAA
PRIMER_RIGHT_9_SEQUENCE=ATCCAAGCTTGTCTGAGAGTGAC
PRIMER_LEFT_9=579,23
PRIMER_RIGHT_9=1468,23
PRIMER_LEFT_9_TM=60.030
PRIMER_RIGHT_9_TM=59.937
PRIMER_LEFT_9_GC_PERCENT=39.130
PRIMER_RIGHT_9_GC_PERCENT=47.826
PRIMER_LEFT_9_SELF_ANY=6.00
PRIMER_RIGHT_9_SELF_ANY=8.00
PRIMER_LEFT_9_SELF_END=3.00
PRIMER_RIGHT_9_SELF_END=3.00
PRIMER_LEFT_9_MISPRIMING_SCORE=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_9_MISPRIMING_SCORE=22.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_LEFT_9_END_STABILITY=6.2000
PRIMER_RIGHT_9_END_STABILITY=6.1000
PRIMER_PAIR_9_COMPL_ANY=6.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_9=890
PRIMER_PAIR_PENALTY_10=0.0983
PRIMER_LEFT_10_PENALTY=0.030116
PRIMER_RIGHT_10_PENALTY=0.068149
PRIMER_LEFT_10_SEQUENCE=CTTTGAAGAAGCAGCTGGATAAA
PRIMER_RIGHT_10_SEQUENCE=TCCATAGCCATCCATTCTCTCTA
PRIMER_LEFT_10=579,23
PRIMER_RIGHT_10=985,23
PRIMER_LEFT_10_TM=60.030
PRIMER_RIGHT_10_TM=60.068
PRIMER_LEFT_10_GC_PERCENT=39.130
PRIMER_RIGHT_10_GC_PERCENT=43.478
PRIMER_LEFT_10_SELF_ANY=6.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=3.00
PRIMER_RIGHT_10_SELF_END=2.00
PRIMER_LEFT_10_MISPRIMING_SCORE=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_10_MISPRIMING_SCORE=11.00, L1MA2 3'-end of L1 repeat (subfamily L1MA2) - a consensus sequence
PRIMER_PAIR_10_MISPRIMING_SCORE=23.00, Tigger2 Autonomous DNA transposon
PRIMER_LEFT_10_END_STABILITY=6.2000
PRIMER_RIGHT_10_END_STABILITY=5.7000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_10=407
PRIMER_PAIR_PENALTY_11=0.1033
PRIMER_LEFT_11_PENALTY=0.095145
PRIMER_RIGHT_11_PENALTY=0.008181
PRIMER_LEFT_11_SEQUENCE=GGGTAGATTTGGAAAAACCTTTG
PRIMER_RIGHT_11_SEQUENCE=AAATTTCTGAAGAAAGTCCTGGG
PRIMER_LEFT_11=561,23
PRIMER_RIGHT_11=832,23
PRIMER_LEFT_11_TM=60.095
PRIMER_RIGHT_11_TM=59.992
PRIMER_LEFT_11_GC_PERCENT=39.130
PRIMER_RIGHT_11_GC_PERCENT=39.130
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=8.00
PRIMER_LEFT_11_SELF_END=2.00
PRIMER_RIGHT_11_SELF_END=2.00
PRIMER_LEFT_11_MISPRIMING_SCORE=11.00, L1MA2 3'-end of L1 repeat (subfamily L1MA2) - a consensus sequence
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_11_MISPRIMING_SCORE=22.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_11_END_STABILITY=7.3000
PRIMER_RIGHT_11_END_STABILITY=9.7000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_11=272
PRIMER_PAIR_PENALTY_12=0.1072
PRIMER_LEFT_12_PENALTY=0.100425
PRIMER_RIGHT_12_PENALTY=0.006805
PRIMER_LEFT_12_SEQUENCE=CTTCAAGGATGTCTCTGCCTAAA
PRIMER_RIGHT_12_SEQUENCE=GCAAGTAGGGCTGAGGATTATTT
PRIMER_LEFT_12=1308,23
PRIMER_RIGHT_12=1562,23
PRIMER_LEFT_12_TM=59.900
PRIMER_RIGHT_12_TM=60.007
PRIMER_LEFT_12_GC_PERCENT=43.478
PRIMER_RIGHT_12_GC_PERCENT=43.478
PRIMER_LEFT_12_SELF_ANY=5.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=3.00
PRIMER_LEFT_12_MISPRIMING_SCORE=12.00, MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_RIGHT_12_MISPRIMING_SCORE=11.00, reverse MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_PAIR_12_MISPRIMING_SCORE=21.00, reverse MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_LEFT_12_END_STABILITY=6.3000
PRIMER_RIGHT_12_END_STABILITY=6.2000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_12=255
PRIMER_PAIR_PENALTY_13=0.1083
PRIMER_LEFT_13_PENALTY=0.041875
PRIMER_RIGHT_13_PENALTY=0.066430
PRIMER_LEFT_13_SEQUENCE=CTGGAACAACGATTAGAACAAGG
PRIMER_RIGHT_13_SEQUENCE=GGCCAGTACCTAAAGCTCTTCTC
PRIMER_LEFT_13=2990,23
PRIMER_RIGHT_13=3366,23
PRIMER_LEFT_13_TM=60.042
PRIMER_RIGHT_13_TM=59.934
PRIMER_LEFT_13_GC_PERCENT=43.478
PRIMER_RIGHT_13_GC_PERCENT=52.174
PRIMER_LEFT_13_SELF_ANY=2.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_13_MISPRIMING_SCORE=12.00, THE1BR THE-1B Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_PAIR_13_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_13_END_STABILITY=8.5000
PRIMER_RIGHT_13_END_STABILITY=6.7000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_13=377
PRIMER_PAIR_PENALTY_14=0.1083
PRIMER_LEFT_14_PENALTY=0.041875
PRIMER_RIGHT_14_PENALTY=0.066430
PRIMER_LEFT_14_SEQUENCE=CTGGAACAACGATTAGAACAAGG
PRIMER_RIGHT_14_SEQUENCE=GCCAGTACCTAAAGCTCTTCTCC
PRIMER_LEFT_14=2990,23
PRIMER_RIGHT_14=3365,23
PRIMER_LEFT_14_TM=60.042
PRIMER_RIGHT_14_TM=59.934
PRIMER_LEFT_14_GC_PERCENT=43.478
PRIMER_RIGHT_14_GC_PERCENT=52.174
PRIMER_LEFT_14_SELF_ANY=2.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_14_MISPRIMING_SCORE=11.00, THE1BR THE-1B Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_PAIR_14_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_14_END_STABILITY=8.5000
PRIMER_RIGHT_14_END_STABILITY=7.9000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_14=376
PRIMER_PAIR_PENALTY_15=0.1084
PRIMER_LEFT_15_PENALTY=0.101560
PRIMER_RIGHT_15_PENALTY=0.006805
PRIMER_LEFT_15_SEQUENCE=TAAATATGCATTGGAACCTACCG
PRIMER_RIGHT_15_SEQUENCE=GCAAGTAGGGCTGAGGATTATTT
PRIMER_LEFT_15=598,23
PRIMER_RIGHT_15=1562,23
PRIMER_LEFT_15_TM=60.102
PRIMER_RIGHT_15_TM=60.007
PRIMER_LEFT_15_GC_PERCENT=39.130
PRIMER_RIGHT_15_GC_PERCENT=43.478
PRIMER_LEFT_15_SELF_ANY=6.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=2.00
PRIMER_RIGHT_15_SELF_END=3.00
PRIMER_LEFT_15_MISPRIMING_SCORE=12.00, MER6 Nonautonomous DNA transposon
PRIMER_RIGHT_15_MISPRIMING_SCORE=11.00, reverse MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_PAIR_15_MISPRIMING_SCORE=20.00, MER6 Nonautonomous DNA transposon
PRIMER_LEFT_15_END_STABILITY=8.9000
PRIMER_RIGHT_15_END_STABILITY=6.2000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_15=965
PRIMER_PAIR_PENALTY_16=0.1097
PRIMER_LEFT_16_PENALTY=0.101560
PRIMER_RIGHT_16_PENALTY=0.008181
PRIMER_LEFT_16_SEQUENCE=TAAATATGCATTGGAACCTACCG
PRIMER_RIGHT_16_SEQUENCE=AAATTTCTGAAGAAAGTCCTGGG
PRIMER_LEFT_16=598,23
PRIMER_RIGHT_16=832,23
PRIMER_LEFT_16_TM=60.102
PRIMER_RIGHT_16_TM=59.992
PRIMER_LEFT_16_GC_PERCENT=39.130
PRIMER_RIGHT_16_GC_PERCENT=39.130
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=8.00
PRIMER_LEFT_16_SELF_END=2.00
PRIMER_RIGHT_16_SELF_END=2.00
PRIMER_LEFT_16_MISPRIMING_SCORE=12.00, MER6 Nonautonomous DNA transposon
PRIMER_RIGHT_16_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_16_MISPRIMING_SCORE=21.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_16_END_STABILITY=8.9000
PRIMER_RIGHT_16_END_STABILITY=9.7000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_16=235
PRIMER_PAIR_PENALTY_17=0.1107
PRIMER_LEFT_17_PENALTY=0.047652
PRIMER_RIGHT_17_PENALTY=0.063026
PRIMER_LEFT_17_SEQUENCE=TATGAATCCCAGGACTTTCTTCA
PRIMER_RIGHT_17_SEQUENCE=ATCCAAGCTTGTCTGAGAGTGAC
PRIMER_LEFT_17=803,23
PRIMER_RIGHT_17=1468,23
PRIMER_LEFT_17_TM=59.952
PRIMER_RIGHT_17_TM=59.937
PRIMER_LEFT_17_GC_PERCENT=39.130
PRIMER_RIGHT_17_GC_PERCENT=47.826
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=8.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=3.00
PRIMER_LEFT_17_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_17_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_17_MISPRIMING_SCORE=23.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_17_END_STABILITY=7.0000
PRIMER_RIGHT_17_END_STABILITY=6.1000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_17=666
PRIMER_PAIR_PENALTY_18=0.1130
PRIMER_LEFT_18_PENALTY=0.065377
PRIMER_RIGHT_18_PENALTY=0.047652
PRIMER_LEFT_18_SEQUENCE=CTACCGTCTATTTTGGAGTGGTG
PRIMER_RIGHT_18_SEQUENCE=TGAAGAAAGTCCTGGGATTCATA
PRIMER_LEFT_18=615,23
PRIMER_RIGHT_18=825,23
PRIMER_LEFT_18_TM=59.935
PRIMER_RIGHT_18_TM=59.952
PRIMER_LEFT_18_GC_PERCENT=47.826
PRIMER_RIGHT_18_GC_PERCENT=39.130
PRIMER_LEFT_18_SELF_ANY=3.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=2.00
PRIMER_LEFT_18_MISPRIMING_SCORE=11.00, reverse L1PB3 3'-end of L1 repeat (subfamily L1PB3) - a consensus sequence
PRIMER_RIGHT_18_MISPRIMING_SCORE=12.00, MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_PAIR_18_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_18_END_STABILITY=8.2000
PRIMER_RIGHT_18_END_STABILITY=5.9000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_18=211
PRIMER_PAIR_PENALTY_19=0.1158
PRIMER_LEFT_19_PENALTY=0.047652
PRIMER_RIGHT_19_PENALTY=0.068149
PRIMER_LEFT_19_SEQUENCE=TATGAATCCCAGGACTTTCTTCA
PRIMER_RIGHT_19_SEQUENCE=TCCATAGCCATCCATTCTCTCTA
PRIMER_LEFT_19=803,23
PRIMER_RIGHT_19=985,23
PRIMER_LEFT_19_TM=59.952
PRIMER_RIGHT_19_TM=60.068
PRIMER_LEFT_19_GC_PERCENT=39.130
PRIMER_RIGHT_19_GC_PERCENT=43.478
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=2.00
PRIMER_LEFT_19_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_19_MISPRIMING_SCORE=11.00, L1MA2 3'-end of L1 repeat (subfamily L1MA2) - a consensus sequence
PRIMER_PAIR_19_MISPRIMING_SCORE=22.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_19_END_STABILITY=7.0000
PRIMER_RIGHT_19_END_STABILITY=5.7000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_19=183
=