PRIMER_SEQUENCE_ID=NM_007079
SEQUENCE=TGACTATCCAGCTCTGAGAGACGGGAGTTTGGAGTTGCCCGCTTTACTTTGGTTGGGTTGGGGGGGGCGGCGGGCTGTTTTGTTCCTTTTCTTTTTTAAGAGTTGGGTTTTCTTTTTTAATTATCCAAACAGTGGGCAGCTTCCTCCCCCACACCCAAGTATTTGCACAATATTTGTGCGGGGTATGGGGGTGGGTTTTTAAATCTCGTTTCTCTTGGACAAGCACAGGGATCTCGTTCTCCTCATTTTTTGGGGGTGTGTGGGGACTTCTCAGGTCGTGTCCCCAGCCTTCTCTGCAGTCCCTTCTGCCCTGCCGGGCCCGTCGGGAGGCGCCATGGCTCGGATGAACCGCCCGGCCCCGGTGGAGGTGAGCTACAAACACATGCGCTTCCTCATCACCCACAACCCCACCAACGCCACGCTCAGCACCTTCATTGAGGACCTGAAGAAGTACGGGGCTACCACTGTGGTGCGTGTGTGTGAAGTGACCTATGACAAAACGCCGCTGGAGAAGGATGGCATCACCGTTGTGGACTGGCCGTTTGACGATGGGGCGCCCCCGCCCGGCAAGGTAGTGGAAGACTGGCTGAGCCTGGTGAAGGCCAAGTTCTGTGAGGCCCCCGGCAGCTGCGTGGCTGTGCACTGCGTGGCGGGCCTGGGCCGGAAGCGCCGCGGAGCCATCAACAGCAAGCAGCTCACCTACCTGGAGAAATACCGGCCCAAACAGAGGCTGCGGTTCAAAGACCCACACACGCACAAGACCCGGTGCTGCGTTATGTAGCTCAGGACCTTGGCTGGGCCTGGTCGTCATGTAGGTCAGGACCTTGGCTGGACCTGGAGGCCCTGCCCAGCCCTGCTCTGCCCAGCCCAGCAGGGGCTCCAGGCCTTGGCTGGCCCCACATCGCCTTTTCCTCCCCGACACCTCCGTGCACTTGTGTCCGAGGAGCGAGGAGCCCCTCGGGCCCTGGGTGGCCTCTGGGCCCTTTCTCCTGTCTCCGCCACTCCCTCTGGCGGCGCTGGCCGTGGCTCTGTCTCTCTGAGGTGGGTCGGGCGCCCTCTGCCCGCCCCCTCCCACACCAGCCAGGCTGGTCTCCTCTAGCCTGTTTGTTGTGGGGTGGGGGTATATTTTGTAACCACTGGGCCCCCAGCCCCTCTTTTGCGACCCCTTGTCCTGACCTGTTCTCGGCACCTTAAATTATTAGACCCCGGGGCAGTCAGGTGCTCCGGACACCCGAAGGCAATAAAACAGGAGCCGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 14101, GC content failed 1224, low tm 2791, high tm 7624, high any compl 4, high end compl 11, high repeat similarity 15, long poly-x seq 85, ok 2347
PRIMER_RIGHT_EXPLAIN=considered 14127, GC content failed 1715, low tm 2644, high tm 7401, high end compl 3, high repeat similarity 1, long poly-x seq 77, ok 2286
PRIMER_PAIR_EXPLAIN=considered 270, unacceptable product size 223, ok 47
PRIMER_PAIR_PENALTY=0.2298
PRIMER_LEFT_PENALTY=0.208920
PRIMER_RIGHT_PENALTY=0.020838
PRIMER_LEFT_SEQUENCE=CAGCTCACCTACCTGGAGAAATA
PRIMER_RIGHT_SEQUENCE=CCGGGGTCTAATAATTTAAGGTG
PRIMER_LEFT=691,23
PRIMER_RIGHT=1208,23
PRIMER_LEFT_TM=59.791
PRIMER_RIGHT_TM=59.979
PRIMER_LEFT_GC_PERCENT=47.826
PRIMER_RIGHT_GC_PERCENT=43.478
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_MISPRIMING_SCORE=11.00, MER21B a consensus
PRIMER_RIGHT_MISPRIMING_SCORE=11.00, L1MA9 3'-end of L1 repeat (subfamily L1MA9) - a consensus sequence
PRIMER_PAIR_MISPRIMING_SCORE=20.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_END_STABILITY=6.2000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=518
PRIMER_PAIR_PENALTY_1=0.3101
PRIMER_LEFT_1_PENALTY=0.208920
PRIMER_RIGHT_1_PENALTY=0.101151
PRIMER_LEFT_1_SEQUENCE=CAGCTCACCTACCTGGAGAAATA
PRIMER_RIGHT_1_SEQUENCE=GGGTCTAATAATTTAAGGTGCCG
PRIMER_LEFT_1=691,23
PRIMER_RIGHT_1=1205,23
PRIMER_LEFT_1_TM=59.791
PRIMER_RIGHT_1_TM=60.101
PRIMER_LEFT_1_GC_PERCENT=47.826
PRIMER_RIGHT_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_MISPRIMING_SCORE=11.00, MER21B a consensus
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_1_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_1_END_STABILITY=6.2000
PRIMER_RIGHT_1_END_STABILITY=11.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=515
PRIMER_PAIR_PENALTY_2=0.3101
PRIMER_LEFT_2_PENALTY=0.208920
PRIMER_RIGHT_2_PENALTY=0.101151
PRIMER_LEFT_2_SEQUENCE=CAGCTCACCTACCTGGAGAAATA
PRIMER_RIGHT_2_SEQUENCE=CGGGGTCTAATAATTTAAGGTGC
PRIMER_LEFT_2=691,23
PRIMER_RIGHT_2=1207,23
PRIMER_LEFT_2_TM=59.791
PRIMER_RIGHT_2_TM=60.101
PRIMER_LEFT_2_GC_PERCENT=47.826
PRIMER_RIGHT_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_MISPRIMING_SCORE=11.00, MER21B a consensus
PRIMER_RIGHT_2_MISPRIMING_SCORE=11.00, MER34 a consensus
PRIMER_PAIR_2_MISPRIMING_SCORE=21.00, MER34 a consensus
PRIMER_LEFT_2_END_STABILITY=6.2000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=517
PRIMER_PAIR_PENALTY_3=0.3104
PRIMER_LEFT_3_PENALTY=0.101439
PRIMER_RIGHT_3_PENALTY=0.208920
PRIMER_LEFT_3_SEQUENCE=GGTGGGTTTTTAAATCTCGTTTC
PRIMER_RIGHT_3_SEQUENCE=TATTTCTCCAGGTAGGTGAGCTG
PRIMER_LEFT_3=189,23
PRIMER_RIGHT_3=713,23
PRIMER_LEFT_3_TM=60.101
PRIMER_RIGHT_3_TM=59.791
PRIMER_LEFT_3_GC_PERCENT=39.130
PRIMER_RIGHT_3_GC_PERCENT=47.826
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, MER7B Nonautonomous DNA transposon
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_3_MISPRIMING_SCORE=20.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=525
PRIMER_PAIR_PENALTY_4=0.4749
PRIMER_LEFT_4_PENALTY=0.101439
PRIMER_RIGHT_4_PENALTY=0.373459
PRIMER_LEFT_4_SEQUENCE=GGTGGGTTTTTAAATCTCGTTTC
PRIMER_RIGHT_4_SEQUENCE=GGTATTTCTCCAGGTAGGTGAGC
PRIMER_LEFT_4=189,23
PRIMER_RIGHT_4=715,23
PRIMER_LEFT_4_TM=60.101
PRIMER_RIGHT_4_TM=60.373
PRIMER_LEFT_4_GC_PERCENT=39.130
PRIMER_RIGHT_4_GC_PERCENT=52.174
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, MER7B Nonautonomous DNA transposon
PRIMER_RIGHT_4_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_4_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=527
PRIMER_PAIR_PENALTY_5=0.4913
PRIMER_LEFT_5_PENALTY=0.470484
PRIMER_RIGHT_5_PENALTY=0.020838
PRIMER_LEFT_5_SEQUENCE=GTGGAGGTGAGCTACAAACACAT
PRIMER_RIGHT_5_SEQUENCE=CCGGGGTCTAATAATTTAAGGTG
PRIMER_LEFT_5=361,23
PRIMER_RIGHT_5=1208,23
PRIMER_LEFT_5_TM=60.470
PRIMER_RIGHT_5_TM=59.979
PRIMER_LEFT_5_GC_PERCENT=47.826
PRIMER_RIGHT_5_GC_PERCENT=43.478
PRIMER_LEFT_5_SELF_ANY=5.00
PRIMER_RIGHT_5_SELF_ANY=5.00
PRIMER_LEFT_5_SELF_END=2.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_MISPRIMING_SCORE=12.00, reverse LTR5 LTR from human endogenous retrovirus 5' LTR, clone HERV-K18
PRIMER_RIGHT_5_MISPRIMING_SCORE=11.00, L1MA9 3'-end of L1 repeat (subfamily L1MA9) - a consensus sequence
PRIMER_PAIR_5_MISPRIMING_SCORE=21.00, reverse MER4C a consensus
PRIMER_LEFT_5_END_STABILITY=6.6000
PRIMER_RIGHT_5_END_STABILITY=7.9000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_5=848
PRIMER_PAIR_PENALTY_6=0.5716
PRIMER_LEFT_6_PENALTY=0.470484
PRIMER_RIGHT_6_PENALTY=0.101151
PRIMER_LEFT_6_SEQUENCE=GTGGAGGTGAGCTACAAACACAT
PRIMER_RIGHT_6_SEQUENCE=GGGTCTAATAATTTAAGGTGCCG
PRIMER_LEFT_6=361,23
PRIMER_RIGHT_6=1205,23
PRIMER_LEFT_6_TM=60.470
PRIMER_RIGHT_6_TM=60.101
PRIMER_LEFT_6_GC_PERCENT=47.826
PRIMER_RIGHT_6_GC_PERCENT=43.478
PRIMER_LEFT_6_SELF_ANY=5.00
PRIMER_RIGHT_6_SELF_ANY=5.00
PRIMER_LEFT_6_SELF_END=2.00
PRIMER_RIGHT_6_SELF_END=2.00
PRIMER_LEFT_6_MISPRIMING_SCORE=12.00, reverse LTR5 LTR from human endogenous retrovirus 5' LTR, clone HERV-K18
PRIMER_RIGHT_6_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_6_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_6_END_STABILITY=6.6000
PRIMER_RIGHT_6_END_STABILITY=11.7000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_6=845
PRIMER_PAIR_PENALTY_7=0.5716
PRIMER_LEFT_7_PENALTY=0.470484
PRIMER_RIGHT_7_PENALTY=0.101151
PRIMER_LEFT_7_SEQUENCE=GTGGAGGTGAGCTACAAACACAT
PRIMER_RIGHT_7_SEQUENCE=CGGGGTCTAATAATTTAAGGTGC
PRIMER_LEFT_7=361,23
PRIMER_RIGHT_7=1207,23
PRIMER_LEFT_7_TM=60.470
PRIMER_RIGHT_7_TM=60.101
PRIMER_LEFT_7_GC_PERCENT=47.826
PRIMER_RIGHT_7_GC_PERCENT=43.478
PRIMER_LEFT_7_SELF_ANY=5.00
PRIMER_RIGHT_7_SELF_ANY=5.00
PRIMER_LEFT_7_SELF_END=2.00
PRIMER_RIGHT_7_SELF_END=2.00
PRIMER_LEFT_7_MISPRIMING_SCORE=12.00, reverse LTR5 LTR from human endogenous retrovirus 5' LTR, clone HERV-K18
PRIMER_RIGHT_7_MISPRIMING_SCORE=11.00, MER34 a consensus
PRIMER_PAIR_7_MISPRIMING_SCORE=22.00, reverse Tigger2 Autonomous DNA transposon
PRIMER_LEFT_7_END_STABILITY=6.6000
PRIMER_RIGHT_7_END_STABILITY=9.4000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_7=847
PRIMER_PAIR_PENALTY_8=0.6794
PRIMER_LEFT_8_PENALTY=0.470484
PRIMER_RIGHT_8_PENALTY=0.208920
PRIMER_LEFT_8_SEQUENCE=GTGGAGGTGAGCTACAAACACAT
PRIMER_RIGHT_8_SEQUENCE=TATTTCTCCAGGTAGGTGAGCTG
PRIMER_LEFT_8=361,23
PRIMER_RIGHT_8=713,23
PRIMER_LEFT_8_TM=60.470
PRIMER_RIGHT_8_TM=59.791
PRIMER_LEFT_8_GC_PERCENT=47.826
PRIMER_RIGHT_8_GC_PERCENT=47.826
PRIMER_LEFT_8_SELF_ANY=5.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=2.00
PRIMER_RIGHT_8_SELF_END=2.00
PRIMER_LEFT_8_MISPRIMING_SCORE=12.00, reverse LTR5 LTR from human endogenous retrovirus 5' LTR, clone HERV-K18
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_8_MISPRIMING_SCORE=23.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_8_END_STABILITY=6.6000
PRIMER_RIGHT_8_END_STABILITY=8.2000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_8=353
PRIMER_PAIR_PENALTY_9=0.7756
PRIMER_LEFT_9_PENALTY=0.566640
PRIMER_RIGHT_9_PENALTY=0.208920
PRIMER_LEFT_9_SEQUENCE=TTTAATTATCCAAACAGTGGGCA
PRIMER_RIGHT_9_SEQUENCE=TATTTCTCCAGGTAGGTGAGCTG
PRIMER_LEFT_9=115,23
PRIMER_RIGHT_9=713,23
PRIMER_LEFT_9_TM=60.567
PRIMER_RIGHT_9_TM=59.791
PRIMER_LEFT_9_GC_PERCENT=34.783
PRIMER_RIGHT_9_GC_PERCENT=47.826
PRIMER_LEFT_9_SELF_ANY=6.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=2.00
PRIMER_RIGHT_9_SELF_END=2.00
PRIMER_LEFT_9_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_9_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_9_END_STABILITY=11.2000
PRIMER_RIGHT_9_END_STABILITY=8.2000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_9=599
PRIMER_PAIR_PENALTY_10=0.8152
PRIMER_LEFT_10_PENALTY=0.208920
PRIMER_RIGHT_10_PENALTY=0.606284
PRIMER_LEFT_10_SEQUENCE=CAGCTCACCTACCTGGAGAAATA
PRIMER_RIGHT_10_SEQUENCE=CCCGGGGTCTAATAATTTAAGGT
PRIMER_LEFT_10=691,23
PRIMER_RIGHT_10=1209,23
PRIMER_LEFT_10_TM=59.791
PRIMER_RIGHT_10_TM=60.606
PRIMER_LEFT_10_GC_PERCENT=47.826
PRIMER_RIGHT_10_GC_PERCENT=43.478
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=2.00
PRIMER_RIGHT_10_SELF_END=2.00
PRIMER_LEFT_10_MISPRIMING_SCORE=11.00, MER21B a consensus
PRIMER_RIGHT_10_MISPRIMING_SCORE=10.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_10_MISPRIMING_SCORE=20.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_10_END_STABILITY=6.2000
PRIMER_RIGHT_10_END_STABILITY=7.9000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_10=519
PRIMER_PAIR_PENALTY_11=0.8439
PRIMER_LEFT_11_PENALTY=0.470484
PRIMER_RIGHT_11_PENALTY=0.373459
PRIMER_LEFT_11_SEQUENCE=GTGGAGGTGAGCTACAAACACAT
PRIMER_RIGHT_11_SEQUENCE=GGTATTTCTCCAGGTAGGTGAGC
PRIMER_LEFT_11=361,23
PRIMER_RIGHT_11=715,23
PRIMER_LEFT_11_TM=60.470
PRIMER_RIGHT_11_TM=60.373
PRIMER_LEFT_11_GC_PERCENT=47.826
PRIMER_RIGHT_11_GC_PERCENT=52.174
PRIMER_LEFT_11_SELF_ANY=5.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=2.00
PRIMER_RIGHT_11_SELF_END=2.00
PRIMER_LEFT_11_MISPRIMING_SCORE=12.00, reverse LTR5 LTR from human endogenous retrovirus 5' LTR, clone HERV-K18
PRIMER_RIGHT_11_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_11_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_11_END_STABILITY=6.6000
PRIMER_RIGHT_11_END_STABILITY=8.2000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_11=355
PRIMER_PAIR_PENALTY_12=0.8467
PRIMER_LEFT_12_PENALTY=0.825895
PRIMER_RIGHT_12_PENALTY=0.020838
PRIMER_LEFT_12_SEQUENCE=TCTCCTCTAGCCTGTTTGTTGTG
PRIMER_RIGHT_12_SEQUENCE=CCGGGGTCTAATAATTTAAGGTG
PRIMER_LEFT_12=1089,23
PRIMER_RIGHT_12=1208,23
PRIMER_LEFT_12_TM=60.826
PRIMER_RIGHT_12_TM=59.979
PRIMER_LEFT_12_GC_PERCENT=47.826
PRIMER_RIGHT_12_GC_PERCENT=43.478
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=5.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_MISPRIMING_SCORE=12.00, THE1BR THE-1B Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_RIGHT_12_MISPRIMING_SCORE=11.00, L1MA9 3'-end of L1 repeat (subfamily L1MA9) - a consensus sequence
PRIMER_PAIR_12_MISPRIMING_SCORE=20.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_12_END_STABILITY=7.0000
PRIMER_RIGHT_12_END_STABILITY=7.9000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_12=120
PRIMER_PAIR_PENALTY_13=0.9270
PRIMER_LEFT_13_PENALTY=0.825895
PRIMER_RIGHT_13_PENALTY=0.101151
PRIMER_LEFT_13_SEQUENCE=TCTCCTCTAGCCTGTTTGTTGTG
PRIMER_RIGHT_13_SEQUENCE=GGGTCTAATAATTTAAGGTGCCG
PRIMER_LEFT_13=1089,23
PRIMER_RIGHT_13=1205,23
PRIMER_LEFT_13_TM=60.826
PRIMER_RIGHT_13_TM=60.101
PRIMER_LEFT_13_GC_PERCENT=47.826
PRIMER_RIGHT_13_GC_PERCENT=43.478
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=5.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=2.00
PRIMER_LEFT_13_MISPRIMING_SCORE=12.00, THE1BR THE-1B Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_RIGHT_13_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_13_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_13_END_STABILITY=7.0000
PRIMER_RIGHT_13_END_STABILITY=11.7000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_13=117
PRIMER_PAIR_PENALTY_14=0.9270
PRIMER_LEFT_14_PENALTY=0.825895
PRIMER_RIGHT_14_PENALTY=0.101151
PRIMER_LEFT_14_SEQUENCE=TCTCCTCTAGCCTGTTTGTTGTG
PRIMER_RIGHT_14_SEQUENCE=CGGGGTCTAATAATTTAAGGTGC
PRIMER_LEFT_14=1089,23
PRIMER_RIGHT_14=1207,23
PRIMER_LEFT_14_TM=60.826
PRIMER_RIGHT_14_TM=60.101
PRIMER_LEFT_14_GC_PERCENT=47.826
PRIMER_RIGHT_14_GC_PERCENT=43.478
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=5.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=2.00
PRIMER_LEFT_14_MISPRIMING_SCORE=12.00, THE1BR THE-1B Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_RIGHT_14_MISPRIMING_SCORE=11.00, MER34 a consensus
PRIMER_PAIR_14_MISPRIMING_SCORE=21.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_14_END_STABILITY=7.0000
PRIMER_RIGHT_14_END_STABILITY=9.4000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_14=119
PRIMER_PAIR_PENALTY_15=0.9273
PRIMER_LEFT_15_PENALTY=0.101439
PRIMER_RIGHT_15_PENALTY=0.825895
PRIMER_LEFT_15_SEQUENCE=GGTGGGTTTTTAAATCTCGTTTC
PRIMER_RIGHT_15_SEQUENCE=CACAACAAACAGGCTAGAGGAGA
PRIMER_LEFT_15=189,23
PRIMER_RIGHT_15=1111,23
PRIMER_LEFT_15_TM=60.101
PRIMER_RIGHT_15_TM=60.826
PRIMER_LEFT_15_GC_PERCENT=39.130
PRIMER_RIGHT_15_GC_PERCENT=47.826
PRIMER_LEFT_15_SELF_ANY=6.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_MISPRIMING_SCORE=12.00, MER7B Nonautonomous DNA transposon
PRIMER_RIGHT_15_MISPRIMING_SCORE=12.00, reverse MLT2B2 Interspersed repeat MLT2B2 - a consensus
PRIMER_PAIR_15_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_15_END_STABILITY=6.7000
PRIMER_RIGHT_15_END_STABILITY=7.9000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_15=923
PRIMER_PAIR_PENALTY_16=0.9401
PRIMER_LEFT_16_PENALTY=0.566640
PRIMER_RIGHT_16_PENALTY=0.373459
PRIMER_LEFT_16_SEQUENCE=TTTAATTATCCAAACAGTGGGCA
PRIMER_RIGHT_16_SEQUENCE=GGTATTTCTCCAGGTAGGTGAGC
PRIMER_LEFT_16=115,23
PRIMER_RIGHT_16=715,23
PRIMER_LEFT_16_TM=60.567
PRIMER_RIGHT_16_TM=60.373
PRIMER_LEFT_16_GC_PERCENT=34.783
PRIMER_RIGHT_16_GC_PERCENT=52.174
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=3.00
PRIMER_LEFT_16_SELF_END=2.00
PRIMER_RIGHT_16_SELF_END=2.00
PRIMER_LEFT_16_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_16_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_16_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_16_END_STABILITY=11.2000
PRIMER_RIGHT_16_END_STABILITY=8.2000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_16=601
PRIMER_PAIR_PENALTY_17=0.9825
PRIMER_LEFT_17_PENALTY=0.101439
PRIMER_RIGHT_17_PENALTY=0.881107
PRIMER_LEFT_17_SEQUENCE=GGTGGGTTTTTAAATCTCGTTTC
PRIMER_RIGHT_17_SEQUENCE=TGGTTACAAAATATACCCCCACC
PRIMER_LEFT_17=189,23
PRIMER_RIGHT_17=1135,23
PRIMER_LEFT_17_TM=60.101
PRIMER_RIGHT_17_TM=60.881
PRIMER_LEFT_17_GC_PERCENT=39.130
PRIMER_RIGHT_17_GC_PERCENT=43.478
PRIMER_LEFT_17_SELF_ANY=6.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_MISPRIMING_SCORE=12.00, MER7B Nonautonomous DNA transposon
PRIMER_RIGHT_17_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_17_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_17_END_STABILITY=6.7000
PRIMER_RIGHT_17_END_STABILITY=9.4000
PRIMER_PAIR_17_COMPL_ANY=6.00
PRIMER_PAIR_17_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_17=947
PRIMER_PAIR_PENALTY_18=1.0063
PRIMER_LEFT_18_PENALTY=0.208920
PRIMER_RIGHT_18_PENALTY=0.797332
PRIMER_LEFT_18_SEQUENCE=CAGCTCACCTACCTGGAGAAATA
PRIMER_RIGHT_18_SEQUENCE=GGGGTCTAATAATTTAAGGTGCC
PRIMER_LEFT_18=691,23
PRIMER_RIGHT_18=1206,23
PRIMER_LEFT_18_TM=59.791
PRIMER_RIGHT_18_TM=59.203
PRIMER_LEFT_18_GC_PERCENT=47.826
PRIMER_RIGHT_18_GC_PERCENT=43.478
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=5.00
PRIMER_LEFT_18_SELF_END=2.00
PRIMER_RIGHT_18_SELF_END=2.00
PRIMER_LEFT_18_MISPRIMING_SCORE=11.00, MER21B a consensus
PRIMER_RIGHT_18_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_18_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_18_END_STABILITY=6.2000
PRIMER_RIGHT_18_END_STABILITY=9.4000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_18=516
PRIMER_PAIR_PENALTY_19=1.0348
PRIMER_LEFT_19_PENALTY=0.208920
PRIMER_RIGHT_19_PENALTY=0.825895
PRIMER_LEFT_19_SEQUENCE=CAGCTCACCTACCTGGAGAAATA
PRIMER_RIGHT_19_SEQUENCE=CACAACAAACAGGCTAGAGGAGA
PRIMER_LEFT_19=691,23
PRIMER_RIGHT_19=1111,23
PRIMER_LEFT_19_TM=59.791
PRIMER_RIGHT_19_TM=60.826
PRIMER_LEFT_19_GC_PERCENT=47.826
PRIMER_RIGHT_19_GC_PERCENT=47.826
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=2.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_MISPRIMING_SCORE=11.00, MER21B a consensus
PRIMER_RIGHT_19_MISPRIMING_SCORE=12.00, reverse MLT2B2 Interspersed repeat MLT2B2 - a consensus
PRIMER_PAIR_19_MISPRIMING_SCORE=21.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_19_END_STABILITY=6.2000
PRIMER_RIGHT_19_END_STABILITY=7.9000
PRIMER_PAIR_19_COMPL_ANY=6.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_19=421
=