PRIMER_SEQUENCE_ID=NM_007207
SEQUENCE=TTACATTTACAGTGTAGCAAAAGAGAAAGTAACTATGTTGCTGCTGGAGATCAATGAAGCCGAGTGAATGGGGGCTGAATGTGCGAGTCCATAGCTGAAGAGGAGCGCCAGATGGTGGAGGAATACACTTATTTATGAAACTGTCTTGAGTTCTTCTTGAATTGCCAGTTTTCAGCCTCCTCATGCCTCCGTCTCCTTTAGACGACAGGGTAGTAGTGGCACTATCTAGGCCCGTCCGACCTCAGGATCTCAACCTTTGTTTAGACTCTAGTTACCTTGGCTCTGCCAACCCAGGCAGTAACAGCCACCCTCCTGTCATCGCCACCACCGTTGTGTCCCTCAAGGCTGCGAATCTGACGTATATGCCCTCATCCAGCGGCTCTGCCCGCTCGCTGAATTGTGGATGCAGCAGTGCCAGCTGCTGCACTGTGGCAACCTACGACAAGGACAATCAGGCCCAAACCCAAGCCATTGCCGCTGGCACCACCACCACTGCCATCGGAACCTCTACCACCTGCCCTGCTAACCAGATGGTCAACAATAATGAGAATACAGGCTCTCTAAGTCCATCAAGTGGGGTGGGCAGCCCTGTGTCAGGGACCCCCAAGCAGCTAGCCAGCATCAAAATAATCTACCCCAATGACTTGGCAAAGAAGATGACCAAATGCAGCAAGAGTCACCTGCCGAGTCAGGGCCCTGTCATCATTGACTGCAGGCCCTTCATGGAGTACAACAAGAGTCACATCCAAGGAGCTGTCCACATTAACTGTGCCGATAAGATCAGCCGGCGGAGACTGCAGCAGGGCAAGATCACTGTCCTAGACTTGATTTCCTGTAGGGAAGGCAAGGACTCTTTCAAGAGGATCTTTTCCAAAGAAATTATAGTTTATGATGAGAATACCAATGAACCAAGCCGAGTGATGCCCTCCCAGCCACTTCACATAGTCCTCGAGTCCCTGAAGAGAGAAGGCAAAGAACCTCTGGTGTTGAAAGGTGGACTTAGTAGTTTTAAGCAGAACCATGAAAACCTCTGTGACAACTCCCTCCAGCTCCAAGAGTGCCGGGAGGTGGGGGGCGGCGCATCCGCGGCCTCGAGCTTGCTACCTCAGCCCATCCCCACCACCCCTGACATCGAGAACGCTGAGCTCACCCCCATCTTGCCCTTCCTGTTCCTTGGCAATGAGCAGGATGCTCAGGACCTGGACACCATGCAGCGGCTGAACATCGGCTACGTCATCAACGTCACCACTCATCTTCCCCTCTACCACTATGAGAAAGGCCTGTTCAACTACAAGCGGCTGCCAGCCACTGACAGCAACAAGCAGAACCTGCGGCAGTACTTTGAAGAGGCTTTTGAGTTCATTGAGGAAGCTCACCAGTGTGGGAAGGGGCTTCTCATCCACTGCCAGGCTGGGGTGTCCCGCTCCGCCACCATCGTCATCGCTTACTTGATGAAGCACACTCGGATGACCATGACTGATGCTTATAAATTTGTCAAAGGCAAACGACCAATTATCTCCCCAAACCTTAACTTCATGGGGCAGTTGCTAGAGTTCGAGGAAGACCTAAACAACGGTGTGACACCGAGAATCCTTACACCAAAGCTGATGGGCGTGGAGACGGTTGTGTGACAATGGTCTGGATGGAAAGGATTGCTGCTCTCCATTAGGAGACAATGAGGAAGGAGGATGGATTCTGGTTTTTTTTCTTTCTTTTTTTTTTTGTAGTTGGGAGTAAGTTTGTGAATGGAAACAAACTTGTTTAAACACTTTATTTTTAACAAGTGTAAGAAGACTATAACTTTTGATGCCATTGAGATTCACCTCCCACAAACTGACAAATTAAGGAGGTTAAAGAAGTAATTTTTTTAAGCCAACAATAAAAATATAATACAACTTGTTTCTCCCCCTTTTCCTTTTAAGCTATTTGTAGAGTTTATGACTAAATAGTCTGTGCAGGTTCATAGACCGAAGATACTACACACTTTAAACCAATTAAAAAGAACCAAAAGTAAATAGAAAAGACATTGAATCACCAAGGCCTGGGATCAACCTGGGCTGTCCACACAGAAAACAAAAACCCAACCAAACCAAGCCCTGTTGTGCTCACTGGTGCAAAGAGAAGATCAGGGCAGCTTAAGTGGTCTAAGAATCCTTCAGGCATTCTTTAAGGAGAAAAAGGATACCTTTGATTTTGTGTGTTTCATGCTCTGGATTTTTTTTTTTTTTCCTTCTCTGGGTTTAAGAGATTTTTTTTGAAATAGTGAGGAACTGACCATTATATGCCTTCACTGGCTTCTTGTGCAATAATATGATGTTTTAAGTGTGCAAACAAGTTAGAGCTGGCAGCTGAATGATAGACAAATAGTGCAAATTTGCCAGCTTGGAGATAGAAAGGAATTCAACAATATATCAAATACTTTCCTTCCCACCTTTTTCCTTTTTTTTTTTTTTTTCTGATTTGATTCTGGTTACAGTGCCATAAACCTTGTTACATATGTATATCAGAATGTAAGAAAAAAAAATTTATTTAAAAATATTTTTCGCAAAAAAAAAAAAAATCTTGGTGTGTTTCTGTTGATTGTGTAAAAATTTATTTTCATTATATAAATGAAACTGG
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 29350, GC content failed 1509, low tm 12706, high tm 9104, high any compl 2, high end compl 31, high repeat similarity 49, long poly-x seq 131, ok 5818
PRIMER_RIGHT_EXPLAIN=considered 29155, GC content failed 1975, low tm 12412, high tm 8830, high end compl 12, high repeat similarity 21, long poly-x seq 164, ok 5741
PRIMER_PAIR_EXPLAIN=considered 504, unacceptable product size 450, high any compl 1, high end compl 4, ok 49
PRIMER_PAIR_PENALTY=0.1121
PRIMER_LEFT_PENALTY=0.003045
PRIMER_RIGHT_PENALTY=0.109069
PRIMER_LEFT_SEQUENCE=CATTATATGCCTTCACTGGCTTC
PRIMER_RIGHT_SEQUENCE=GGCACTGTAACCAGAATCAAATC
PRIMER_LEFT=2275,23
PRIMER_RIGHT=2479,23
PRIMER_LEFT_TM=60.003
PRIMER_RIGHT_TM=59.891
PRIMER_LEFT_GC_PERCENT=43.478
PRIMER_RIGHT_GC_PERCENT=43.478
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_MISPRIMING_SCORE=12.00, MER21B a consensus
PRIMER_RIGHT_MISPRIMING_SCORE=12.00, reverse MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_PAIR_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=205
PRIMER_PAIR_PENALTY_1=0.1124
PRIMER_LEFT_1_PENALTY=0.074918
PRIMER_RIGHT_1_PENALTY=0.037505
PRIMER_LEFT_1_SEQUENCE=CTGGTGTTGAAAGGTGGACTTAG
PRIMER_RIGHT_1_SEQUENCE=TAAGGTTTGGGGAGATAATTGGT
PRIMER_LEFT_1=980,23
PRIMER_RIGHT_1=1529,23
PRIMER_LEFT_1_TM=60.075
PRIMER_RIGHT_1_TM=59.962
PRIMER_LEFT_1_GC_PERCENT=47.826
PRIMER_RIGHT_1_GC_PERCENT=39.130
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_MISPRIMING_SCORE=12.00, reverse MER21B a consensus
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_1_MISPRIMING_SCORE=20.00, reverse MER4C a consensus
PRIMER_LEFT_1_END_STABILITY=6.0000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=550
PRIMER_PAIR_PENALTY_2=0.1522
PRIMER_LEFT_2_PENALTY=0.043085
PRIMER_RIGHT_2_PENALTY=0.109069
PRIMER_LEFT_2_SEQUENCE=GAAGACCTAAACAACGGTGTGAC
PRIMER_RIGHT_2_SEQUENCE=GGCACTGTAACCAGAATCAAATC
PRIMER_LEFT_2=1559,23
PRIMER_RIGHT_2=2479,23
PRIMER_LEFT_2_TM=59.957
PRIMER_RIGHT_2_TM=59.891
PRIMER_LEFT_2_GC_PERCENT=47.826
PRIMER_RIGHT_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, reverse MLT1f (MLT1f subfamily) - consensus sequence
PRIMER_RIGHT_2_MISPRIMING_SCORE=12.00, reverse MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_PAIR_2_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_2_END_STABILITY=6.1000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=921
PRIMER_PAIR_PENALTY_3=0.1901
PRIMER_LEFT_3_PENALTY=0.074918
PRIMER_RIGHT_3_PENALTY=0.115144
PRIMER_LEFT_3_SEQUENCE=CTGGTGTTGAAAGGTGGACTTAG
PRIMER_RIGHT_3_SEQUENCE=TCAATGAACTCAAAAGCCTCTTC
PRIMER_LEFT_3=980,23
PRIMER_RIGHT_3=1365,23
PRIMER_LEFT_3_TM=60.075
PRIMER_RIGHT_3_TM=59.885
PRIMER_LEFT_3_GC_PERCENT=47.826
PRIMER_RIGHT_3_GC_PERCENT=39.130
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, reverse MER21B a consensus
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_3_MISPRIMING_SCORE=23.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_3_END_STABILITY=6.0000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=386
PRIMER_PAIR_PENALTY_4=0.2108
PRIMER_LEFT_4_PENALTY=0.101745
PRIMER_RIGHT_4_PENALTY=0.109069
PRIMER_LEFT_4_SEQUENCE=CCAGCTTGGAGATAGAAAGGAAT
PRIMER_RIGHT_4_SEQUENCE=GGCACTGTAACCAGAATCAAATC
PRIMER_LEFT_4=2377,23
PRIMER_RIGHT_4=2479,23
PRIMER_LEFT_4_TM=60.102
PRIMER_RIGHT_4_TM=59.891
PRIMER_LEFT_4_GC_PERCENT=43.478
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, reverse MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_PAIR_4_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=103
PRIMER_PAIR_PENALTY_5=0.2115
PRIMER_LEFT_5_PENALTY=0.102474
PRIMER_RIGHT_5_PENALTY=0.109069
PRIMER_LEFT_5_SEQUENCE=ATAAATTTGTCAAAGGCAAACGA
PRIMER_RIGHT_5_SEQUENCE=GGCACTGTAACCAGAATCAAATC
PRIMER_LEFT_5=1485,23
PRIMER_RIGHT_5=2479,23
PRIMER_LEFT_5_TM=59.898
PRIMER_RIGHT_5_TM=59.891
PRIMER_LEFT_5_GC_PERCENT=30.435
PRIMER_RIGHT_5_GC_PERCENT=43.478
PRIMER_LEFT_5_SELF_ANY=7.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=2.00
PRIMER_LEFT_5_MISPRIMING_SCORE=12.00, MARINER1 Human mariner
PRIMER_RIGHT_5_MISPRIMING_SCORE=12.00, reverse MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_PAIR_5_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_5_END_STABILITY=8.4000
PRIMER_RIGHT_5_END_STABILITY=6.9000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_5=995
PRIMER_PAIR_PENALTY_6=0.2189
PRIMER_LEFT_6_PENALTY=0.181407
PRIMER_RIGHT_6_PENALTY=0.037505
PRIMER_LEFT_6_SEQUENCE=CCACTTCACATAGTCCTCGAGTC
PRIMER_RIGHT_6_SEQUENCE=TAAGGTTTGGGGAGATAATTGGT
PRIMER_LEFT_6=932,23
PRIMER_RIGHT_6=1529,23
PRIMER_LEFT_6_TM=60.181
PRIMER_RIGHT_6_TM=59.962
PRIMER_LEFT_6_GC_PERCENT=52.174
PRIMER_RIGHT_6_GC_PERCENT=39.130
PRIMER_LEFT_6_SELF_ANY=6.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=3.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_6_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_6_MISPRIMING_SCORE=22.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_6_END_STABILITY=6.1000
PRIMER_RIGHT_6_END_STABILITY=8.2000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_6=598
PRIMER_PAIR_PENALTY_7=0.2219
PRIMER_LEFT_7_PENALTY=0.184441
PRIMER_RIGHT_7_PENALTY=0.037505
PRIMER_LEFT_7_SEQUENCE=CAAAGAACCTCTGGTGTTGAAAG
PRIMER_RIGHT_7_SEQUENCE=TAAGGTTTGGGGAGATAATTGGT
PRIMER_LEFT_7=970,23
PRIMER_RIGHT_7=1529,23
PRIMER_LEFT_7_TM=60.184
PRIMER_RIGHT_7_TM=59.962
PRIMER_LEFT_7_GC_PERCENT=43.478
PRIMER_RIGHT_7_GC_PERCENT=39.130
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_7_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_7_MISPRIMING_SCORE=21.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_7_END_STABILITY=7.0000
PRIMER_RIGHT_7_END_STABILITY=8.2000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_7=560
PRIMER_PAIR_PENALTY_8=0.2255
PRIMER_LEFT_8_PENALTY=0.187985
PRIMER_RIGHT_8_PENALTY=0.037505
PRIMER_LEFT_8_SEQUENCE=GAGAAAGGCCTGTTCAACTACAA
PRIMER_RIGHT_8_SEQUENCE=TAAGGTTTGGGGAGATAATTGGT
PRIMER_LEFT_8=1271,23
PRIMER_RIGHT_8=1529,23
PRIMER_LEFT_8_TM=59.812
PRIMER_RIGHT_8_TM=59.962
PRIMER_LEFT_8_GC_PERCENT=43.478
PRIMER_RIGHT_8_GC_PERCENT=39.130
PRIMER_LEFT_8_SELF_ANY=8.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_8_MISPRIMING_SCORE=23.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_8_END_STABILITY=6.0000
PRIMER_RIGHT_8_END_STABILITY=8.2000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_8=259
PRIMER_PAIR_PENALTY_9=0.2313
PRIMER_LEFT_9_PENALTY=0.043085
PRIMER_RIGHT_9_PENALTY=0.188226
PRIMER_LEFT_9_SEQUENCE=GAAGACCTAAACAACGGTGTGAC
PRIMER_RIGHT_9_SEQUENCE=AATAGCTTAAAAGGAAAAGGGGG
PRIMER_LEFT_9=1559,23
PRIMER_RIGHT_9=1926,23
PRIMER_LEFT_9_TM=59.957
PRIMER_RIGHT_9_TM=60.188
PRIMER_LEFT_9_GC_PERCENT=47.826
PRIMER_RIGHT_9_GC_PERCENT=39.130
PRIMER_LEFT_9_SELF_ANY=6.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=3.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_MISPRIMING_SCORE=12.00, reverse MLT1f (MLT1f subfamily) - consensus sequence
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, MER4C a consensus
PRIMER_PAIR_9_MISPRIMING_SCORE=20.00, reverse MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_LEFT_9_END_STABILITY=6.1000
PRIMER_RIGHT_9_END_STABILITY=12.4000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_9=368
PRIMER_PAIR_PENALTY_10=0.2562
PRIMER_LEFT_10_PENALTY=0.206665
PRIMER_RIGHT_10_PENALTY=0.049500
PRIMER_LEFT_10_SEQUENCE=CAGCATCAAAATAATCTACCCCA
PRIMER_RIGHT_10_SEQUENCE=ACTAAGTCCACCTTTCAACACCA
PRIMER_LEFT_10=616,23
PRIMER_RIGHT_10=1003,23
PRIMER_LEFT_10_TM=60.207
PRIMER_RIGHT_10_TM=59.950
PRIMER_LEFT_10_GC_PERCENT=39.130
PRIMER_RIGHT_10_GC_PERCENT=43.478
PRIMER_LEFT_10_SELF_ANY=3.00
PRIMER_RIGHT_10_SELF_ANY=5.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_MISPRIMING_SCORE=12.00, reverse MER6 Nonautonomous DNA transposon
PRIMER_RIGHT_10_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_10_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_10_END_STABILITY=11.2000
PRIMER_RIGHT_10_END_STABILITY=8.2000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_10=388
PRIMER_PAIR_PENALTY_11=0.2631
PRIMER_LEFT_11_PENALTY=0.074918
PRIMER_RIGHT_11_PENALTY=0.188226
PRIMER_LEFT_11_SEQUENCE=CTGGTGTTGAAAGGTGGACTTAG
PRIMER_RIGHT_11_SEQUENCE=AATAGCTTAAAAGGAAAAGGGGG
PRIMER_LEFT_11=980,23
PRIMER_RIGHT_11=1926,23
PRIMER_LEFT_11_TM=60.075
PRIMER_RIGHT_11_TM=60.188
PRIMER_LEFT_11_GC_PERCENT=47.826
PRIMER_RIGHT_11_GC_PERCENT=39.130
PRIMER_LEFT_11_SELF_ANY=3.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=3.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_MISPRIMING_SCORE=12.00, reverse MER21B a consensus
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, MER4C a consensus
PRIMER_PAIR_11_MISPRIMING_SCORE=21.00, MER4C a consensus
PRIMER_LEFT_11_END_STABILITY=6.0000
PRIMER_RIGHT_11_END_STABILITY=12.4000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_11=947
PRIMER_PAIR_PENALTY_12=0.2666
PRIMER_LEFT_12_PENALTY=0.074918
PRIMER_RIGHT_12_PENALTY=0.191645
PRIMER_LEFT_12_SEQUENCE=CTGGTGTTGAAAGGTGGACTTAG
PRIMER_RIGHT_12_SEQUENCE=TCTTCCTCGAACTCTAGCAACTG
PRIMER_LEFT_12=980,23
PRIMER_RIGHT_12=1563,23
PRIMER_LEFT_12_TM=60.075
PRIMER_RIGHT_12_TM=60.192
PRIMER_LEFT_12_GC_PERCENT=47.826
PRIMER_RIGHT_12_GC_PERCENT=47.826
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=6.00
PRIMER_LEFT_12_SELF_END=3.00
PRIMER_RIGHT_12_SELF_END=2.00
PRIMER_LEFT_12_MISPRIMING_SCORE=12.00, reverse MER21B a consensus
PRIMER_RIGHT_12_MISPRIMING_SCORE=12.00, L1MC2 3'-end of L1 repeat (subfamily L1MC2) - a consensus sequence
PRIMER_PAIR_12_MISPRIMING_SCORE=22.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_12_END_STABILITY=6.0000
PRIMER_RIGHT_12_END_STABILITY=6.7000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_12=584
PRIMER_PAIR_PENALTY_13=0.2843
PRIMER_LEFT_13_PENALTY=0.234797
PRIMER_RIGHT_13_PENALTY=0.049500
PRIMER_LEFT_13_SEQUENCE=TTCTTCTTGAATTGCCAGTTTTC
PRIMER_RIGHT_13_SEQUENCE=ACTAAGTCCACCTTTCAACACCA
PRIMER_LEFT_13=150,23
PRIMER_RIGHT_13=1003,23
PRIMER_LEFT_13_TM=59.765
PRIMER_RIGHT_13_TM=59.950
PRIMER_LEFT_13_GC_PERCENT=34.783
PRIMER_RIGHT_13_GC_PERCENT=43.478
PRIMER_LEFT_13_SELF_ANY=5.00
PRIMER_RIGHT_13_SELF_ANY=5.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_MISPRIMING_SCORE=12.00, reverse MER4C a consensus
PRIMER_RIGHT_13_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_13_MISPRIMING_SCORE=22.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_13_END_STABILITY=7.3000
PRIMER_RIGHT_13_END_STABILITY=8.2000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_13=854
PRIMER_PAIR_PENALTY_14=0.2907
PRIMER_LEFT_14_PENALTY=0.102474
PRIMER_RIGHT_14_PENALTY=0.188226
PRIMER_LEFT_14_SEQUENCE=ATAAATTTGTCAAAGGCAAACGA
PRIMER_RIGHT_14_SEQUENCE=AATAGCTTAAAAGGAAAAGGGGG
PRIMER_LEFT_14=1485,23
PRIMER_RIGHT_14=1926,23
PRIMER_LEFT_14_TM=59.898
PRIMER_RIGHT_14_TM=60.188
PRIMER_LEFT_14_GC_PERCENT=30.435
PRIMER_RIGHT_14_GC_PERCENT=39.130
PRIMER_LEFT_14_SELF_ANY=7.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_MISPRIMING_SCORE=12.00, MARINER1 Human mariner
PRIMER_RIGHT_14_MISPRIMING_SCORE=12.00, MER4C a consensus
PRIMER_PAIR_14_MISPRIMING_SCORE=23.00, MER4C a consensus
PRIMER_LEFT_14_END_STABILITY=8.4000
PRIMER_RIGHT_14_END_STABILITY=12.4000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_14=442
PRIMER_PAIR_PENALTY_15=0.2966
PRIMER_LEFT_15_PENALTY=0.181407
PRIMER_RIGHT_15_PENALTY=0.115144
PRIMER_LEFT_15_SEQUENCE=CCACTTCACATAGTCCTCGAGTC
PRIMER_RIGHT_15_SEQUENCE=TCAATGAACTCAAAAGCCTCTTC
PRIMER_LEFT_15=932,23
PRIMER_RIGHT_15=1365,23
PRIMER_LEFT_15_TM=60.181
PRIMER_RIGHT_15_TM=59.885
PRIMER_LEFT_15_GC_PERCENT=52.174
PRIMER_RIGHT_15_GC_PERCENT=39.130
PRIMER_LEFT_15_SELF_ANY=6.00
PRIMER_RIGHT_15_SELF_ANY=5.00
PRIMER_LEFT_15_SELF_END=3.00
PRIMER_RIGHT_15_SELF_END=3.00
PRIMER_LEFT_15_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_15_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_15_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_15_END_STABILITY=6.1000
PRIMER_RIGHT_15_END_STABILITY=6.7000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_15=434
PRIMER_PAIR_PENALTY_16=0.3218
PRIMER_LEFT_16_PENALTY=0.206665
PRIMER_RIGHT_16_PENALTY=0.115144
PRIMER_LEFT_16_SEQUENCE=CAGCATCAAAATAATCTACCCCA
PRIMER_RIGHT_16_SEQUENCE=TCAATGAACTCAAAAGCCTCTTC
PRIMER_LEFT_16=616,23
PRIMER_RIGHT_16=1365,23
PRIMER_LEFT_16_TM=60.207
PRIMER_RIGHT_16_TM=59.885
PRIMER_LEFT_16_GC_PERCENT=39.130
PRIMER_RIGHT_16_GC_PERCENT=39.130
PRIMER_LEFT_16_SELF_ANY=3.00
PRIMER_RIGHT_16_SELF_ANY=5.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=3.00
PRIMER_LEFT_16_MISPRIMING_SCORE=12.00, reverse MER6 Nonautonomous DNA transposon
PRIMER_RIGHT_16_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_16_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_16_END_STABILITY=11.2000
PRIMER_RIGHT_16_END_STABILITY=6.7000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_16=750
PRIMER_PAIR_PENALTY_17=0.3656
PRIMER_LEFT_17_PENALTY=0.003045
PRIMER_RIGHT_17_PENALTY=0.362536
PRIMER_LEFT_17_SEQUENCE=CATTATATGCCTTCACTGGCTTC
PRIMER_RIGHT_17_SEQUENCE=ACACAATCAACAGAAACACACCA
PRIMER_LEFT_17=2275,23
PRIMER_RIGHT_17=2585,23
PRIMER_LEFT_17_TM=60.003
PRIMER_RIGHT_17_TM=60.363
PRIMER_LEFT_17_GC_PERCENT=43.478
PRIMER_RIGHT_17_GC_PERCENT=39.130
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=2.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_MISPRIMING_SCORE=12.00, MER21B a consensus
PRIMER_RIGHT_17_MISPRIMING_SCORE=12.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_17_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_17_END_STABILITY=8.2000
PRIMER_RIGHT_17_END_STABILITY=8.2000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_17=311
PRIMER_PAIR_PENALTY_18=0.3678
PRIMER_LEFT_18_PENALTY=0.074918
PRIMER_RIGHT_18_PENALTY=0.292894
PRIMER_LEFT_18_SEQUENCE=CTGGTGTTGAAAGGTGGACTTAG
PRIMER_RIGHT_18_SEQUENCE=AAAAGCCTCTTCAAAGTACTGCC
PRIMER_LEFT_18=980,23
PRIMER_RIGHT_18=1354,23
PRIMER_LEFT_18_TM=60.075
PRIMER_RIGHT_18_TM=60.293
PRIMER_LEFT_18_GC_PERCENT=47.826
PRIMER_RIGHT_18_GC_PERCENT=43.478
PRIMER_LEFT_18_SELF_ANY=3.00
PRIMER_RIGHT_18_SELF_ANY=6.00
PRIMER_LEFT_18_SELF_END=3.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_MISPRIMING_SCORE=12.00, reverse MER21B a consensus
PRIMER_RIGHT_18_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_18_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_18_END_STABILITY=6.0000
PRIMER_RIGHT_18_END_STABILITY=9.7000
PRIMER_PAIR_18_COMPL_ANY=6.00
PRIMER_PAIR_18_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_18=375
PRIMER_PAIR_PENALTY_19=0.3696
PRIMER_LEFT_19_PENALTY=0.181407
PRIMER_RIGHT_19_PENALTY=0.188226
PRIMER_LEFT_19_SEQUENCE=CCACTTCACATAGTCCTCGAGTC
PRIMER_RIGHT_19_SEQUENCE=AATAGCTTAAAAGGAAAAGGGGG
PRIMER_LEFT_19=932,23
PRIMER_RIGHT_19=1926,23
PRIMER_LEFT_19_TM=60.181
PRIMER_RIGHT_19_TM=60.188
PRIMER_LEFT_19_GC_PERCENT=52.174
PRIMER_RIGHT_19_GC_PERCENT=39.130
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=3.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_19_MISPRIMING_SCORE=12.00, MER4C a consensus
PRIMER_PAIR_19_MISPRIMING_SCORE=20.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_19_END_STABILITY=6.1000
PRIMER_RIGHT_19_END_STABILITY=12.4000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_19=995
=