PRIMER_SEQUENCE_ID=NM_013315
SEQUENCE=AAGGCAGGGGGCGGGGCGTCTCCGAGCGGCGGGGCCAAGGGAGGGCACAACAGCTGCTACCTGAACAGTTTCTGACCCAACAGTTACCCAGCGCCGGACTCGCTGCGCCCCGGCGGCTCTAGGGACCCCCGGCGCCTACACTTAGCTCCGCGCCCGAGAGAATGTTGGACCGACGACACAAGACCTCAGACTTGTGTTATTCTAGCAGCTGAACACACCCCAGGCTCTTCTGACCGGCAGTGGCTCTGGAAGCAGTCTGGTGTATAGAGTTATGGATTCACTACCAGATTCTACTGTATGCTCTTGACAACTATGACCACAATGGTCCACCCACAAATGAATTATCAGGAGTGAACCCAGAGGCACGTATGAATGAAAGTCCTGATCCGACTGACCTGGCGGGAGTCATCATTGAGCTCGGCCCCAATGACAGTCCACAGACAAGTGAATTTAAAGGAGCAACCGAGGAGGCACCTGCGAAAGAAAGCCCACACACAAGTGAATTTAAAGGAGCAGCCCGGGTGTCACCTATCAGTGAAAGTGTGTTAGCACGACTTTCCAAGTTTGAAGTTGAAGATGCTGAAAATGTTGCTTCATATGACAGCAAGATTAAGAAAATTGTGCATTCAATTGTATCATCCTTTGCATTTGGACTATTTGGAGTTTTCCTGGTCTTACTGGATGTCACTCTCATCCTTGCCGACCTAATTTTCACTGACAGCAAACTTTATATTCCTTTGGAGTATCGTTCTATTTCTCTAGCTATTGCCTTATTTTTTCTCATGGATGTTCTTCTTCGAGTATTTGTAGAAAGGAGACAGCAGTATTTTTCTGACTTATTTAACATTTTAGATACTGCCATTATTGTGATTCTTCTGCTGGTTGATGTCGTTTACATTTTTTTTGACATTAAGTTGCTTAGGAATATTCCCAGATGGACACATTTACTTCGACTTCTACGACTTATTATTCTGTTAAGAATTTTTCATCTGTTTCATCAAAAAAGACAACTTGAAAAGCTGATAAGAAGGCGGGTTTCAGAAAACAAAAGGCGATACACAAGGGATGGATTTGACCTAGACCTCACTTACGTTACAGAACGTATTATTGCTATGTCATTTCCATCTTCTGGAAGGCAGTCTTTCTATAGAAATCCAATCAAGGAAGTTGTGCGGTTTCTAGATAAGAAACACCGAAACCACTATCGAGTCTACAATCTATGCAGTGAAAGAGCTTACGATCCTAAGCACTTCCATAATAGGGTCGTTAGAATCATGATTGATGATCATAATGTCCCCACTCTACATCAGATGGTGGTTTTCACCAAGGAAGTAAATGAGTGGATGGCTCAAGATCTTGAAAACATCGTAGCGATTCACTGTAAAGGAGGCACAGATAGAACAGGAACTATGGTTTGTGCCTTCCTTATTGCCTCTGAAATATGTTCAACTGCAAAGGAAAGCCTGTATTATTTTGGAGAAAGGCGAACAGATAAAACCCACAGCGAAAAATTTCAGGGAGTAGAAACTCCTTCTCAGAAGAGATATGTTGCATATTTTGCACAAGTGAAACATCTCTACAACTGGAATCTCCCTCCAAGACGGATACTCTTTATAAAACACTTCATTATTTATTCGATTCCTCGTTATGTACGTGATCTAAAAATCCAAATAGAAATGGAGAAAAAGGTTGTCTTTTCCACTATTTCATTAGGAAAATGTTCGGTACTTGATAACATTACAACAGACAAAATATTAATTGATGTATTCGACGGTCTACCTCTGTATGATGATGTGAAAGTGCAGTTTTTCTATTCGAATCTTCCTACATACTATGACAATTGCTCATTTTACTTCTGGTTGCACACATCTTTTATTGAAAATAACAGGCTTTATCTACCAAAAAATGAATTGGATAATCTACATAAACAAAAAGCACGGAGAATTTATCCATCAGATTTTGCCGTGGAGATACTTTTTGGCGAGAAAATGACTTCCAGTGATGTTGTAGCTGGATCCGATTAAGTATAGCTCCCCCTTCCCCTTCTGGGAAAGAATTATGTTCTTTCCAACCCTGCCACATGTTCATATATCCTAAATCTATCCTAAATGTTCCTTGAAGTATTTATTTATGTTTATATATGTTTATATATGTTCTTCATAAATCTATTACATATATATAGATAAAATGTCAGTGTCTTTCTTCTTTTTTGAAGGTACATACTTCACAGTTTCCATCTTGTATTTACCTAAATTTGGAACACAGTATGCAGGAACATCAGGCATCATTTTGAAGAACTTTGAAATAGAACTTTCAAAGCAAAAACTTGAGATATTCAAAAATTGTGATTCCCCAACACCCACTTACTTACATATTTTTGGTCAAGTATTTATTCCCTGCTTTGGTTCACATTTGTAATTTCAGATTTATTAGAAAGCTAATTTATATATTTTTCTTCCCCTTCATTTACAAACTGTCTGTTAACAGATTGGCAACCAAGACTAAGTTTTAAATCCAGAAGAGAGAAATGTTTCACGCAAGCAGTCCCCCAACTCCCAACACACACTCCCTTTCATTCCGAGCACTAAATAGGTAGCTTACTTGACAAGCTTCCTTTAATTACACACAGACATGGGGGTTGGGGTAAGAAGATGGTGGTAAATATGAAAAGTAATCTTTAATAACTTCTGCTTTTGTATAAAATTGTAAGTGAAGTCAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 31472, GC content failed 2154, low tm 18185, high tm 4600, high any compl 4, high end compl 25, high repeat similarity 28, long poly-x seq 105, ok 6371
PRIMER_RIGHT_EXPLAIN=considered 31397, GC content failed 2586, low tm 18142, high tm 4274, high end compl 5, high repeat similarity 10, long poly-x seq 117, ok 6263
PRIMER_PAIR_EXPLAIN=considered 280, unacceptable product size 230, high end compl 4, ok 46
PRIMER_PAIR_PENALTY=0.0283
PRIMER_LEFT_PENALTY=0.014220
PRIMER_RIGHT_PENALTY=0.014092
PRIMER_LEFT_SEQUENCE=AAGTAAATGAGTGGATGGCTCAA
PRIMER_RIGHT_SEQUENCE=GATATATGAACATGTGGCAGGGT
PRIMER_LEFT=1329,23
PRIMER_RIGHT=2092,23
PRIMER_LEFT_TM=60.014
PRIMER_RIGHT_TM=59.986
PRIMER_LEFT_GC_PERCENT=39.130
PRIMER_RIGHT_GC_PERCENT=43.478
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_MISPRIMING_SCORE=12.00, L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_RIGHT_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=9.1000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=764
PRIMER_PAIR_PENALTY_1=0.0338
PRIMER_LEFT_1_PENALTY=0.019662
PRIMER_RIGHT_1_PENALTY=0.014092
PRIMER_LEFT_1_SEQUENCE=TGTATTATTTTGGAGAAAGGCGA
PRIMER_RIGHT_1_SEQUENCE=GATATATGAACATGTGGCAGGGT
PRIMER_LEFT_1=1464,23
PRIMER_RIGHT_1=2092,23
PRIMER_LEFT_1_TM=59.980
PRIMER_RIGHT_1_TM=59.986
PRIMER_LEFT_1_GC_PERCENT=34.783
PRIMER_RIGHT_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_MISPRIMING_SCORE=12.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_1_MISPRIMING_SCORE=21.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_1_END_STABILITY=11.4000
PRIMER_RIGHT_1_END_STABILITY=9.1000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=629
PRIMER_PAIR_PENALTY_2=0.0339
PRIMER_LEFT_2_PENALTY=0.014220
PRIMER_RIGHT_2_PENALTY=0.019662
PRIMER_LEFT_2_SEQUENCE=AAGTAAATGAGTGGATGGCTCAA
PRIMER_RIGHT_2_SEQUENCE=TCGCCTTTCTCCAAAATAATACA
PRIMER_LEFT_2=1329,23
PRIMER_RIGHT_2=1486,23
PRIMER_LEFT_2_TM=60.014
PRIMER_RIGHT_2_TM=59.980
PRIMER_LEFT_2_GC_PERCENT=39.130
PRIMER_RIGHT_2_GC_PERCENT=34.783
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_RIGHT_2_MISPRIMING_SCORE=12.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_2_MISPRIMING_SCORE=23.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=5.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=158
PRIMER_PAIR_PENALTY_3=0.0346
PRIMER_LEFT_3_PENALTY=0.020535
PRIMER_RIGHT_3_PENALTY=0.014092
PRIMER_LEFT_3_SEQUENCE=GCTATGTCATTTCCATCTTCTGG
PRIMER_RIGHT_3_SEQUENCE=GATATATGAACATGTGGCAGGGT
PRIMER_LEFT_3=1109,23
PRIMER_RIGHT_3=2092,23
PRIMER_LEFT_3_TM=59.979
PRIMER_RIGHT_3_TM=59.986
PRIMER_LEFT_3_GC_PERCENT=43.478
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, reverse Tigger2 Autonomous DNA transposon
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_3_MISPRIMING_SCORE=22.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=9.1000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=984
PRIMER_PAIR_PENALTY_4=0.0348
PRIMER_LEFT_4_PENALTY=0.020535
PRIMER_RIGHT_4_PENALTY=0.014220
PRIMER_LEFT_4_SEQUENCE=GCTATGTCATTTCCATCTTCTGG
PRIMER_RIGHT_4_SEQUENCE=TTGAGCCATCCACTCATTTACTT
PRIMER_LEFT_4=1109,23
PRIMER_RIGHT_4=1351,23
PRIMER_LEFT_4_TM=59.979
PRIMER_RIGHT_4_TM=60.014
PRIMER_LEFT_4_GC_PERCENT=43.478
PRIMER_RIGHT_4_GC_PERCENT=39.130
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, reverse Tigger2 Autonomous DNA transposon
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_PAIR_4_MISPRIMING_SCORE=23.00, L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=5.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=243
PRIMER_PAIR_PENALTY_5=0.0402
PRIMER_LEFT_5_PENALTY=0.020535
PRIMER_RIGHT_5_PENALTY=0.019662
PRIMER_LEFT_5_SEQUENCE=GCTATGTCATTTCCATCTTCTGG
PRIMER_RIGHT_5_SEQUENCE=TCGCCTTTCTCCAAAATAATACA
PRIMER_LEFT_5=1109,23
PRIMER_RIGHT_5=1486,23
PRIMER_LEFT_5_TM=59.979
PRIMER_RIGHT_5_TM=59.980
PRIMER_LEFT_5_GC_PERCENT=43.478
PRIMER_RIGHT_5_GC_PERCENT=34.783
PRIMER_LEFT_5_SELF_ANY=5.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_MISPRIMING_SCORE=12.00, reverse Tigger2 Autonomous DNA transposon
PRIMER_RIGHT_5_MISPRIMING_SCORE=12.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_5_MISPRIMING_SCORE=22.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_5_END_STABILITY=8.2000
PRIMER_RIGHT_5_END_STABILITY=5.6000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_5=378
PRIMER_PAIR_PENALTY_6=0.0411
PRIMER_LEFT_6_PENALTY=0.020535
PRIMER_RIGHT_6_PENALTY=0.020535
PRIMER_LEFT_6_SEQUENCE=GCTATGTCATTTCCATCTTCTGG
PRIMER_RIGHT_6_SEQUENCE=ATCTTGAGCCATCCACTCATTTA
PRIMER_LEFT_6=1109,23
PRIMER_RIGHT_6=1354,23
PRIMER_LEFT_6_TM=59.979
PRIMER_RIGHT_6_TM=59.979
PRIMER_LEFT_6_GC_PERCENT=43.478
PRIMER_RIGHT_6_GC_PERCENT=39.130
PRIMER_LEFT_6_SELF_ANY=5.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=2.00
PRIMER_LEFT_6_MISPRIMING_SCORE=12.00, reverse Tigger2 Autonomous DNA transposon
PRIMER_RIGHT_6_MISPRIMING_SCORE=11.00, L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence
PRIMER_PAIR_6_MISPRIMING_SCORE=21.00, L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_LEFT_6_END_STABILITY=8.2000
PRIMER_RIGHT_6_END_STABILITY=6.2000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_6=246
PRIMER_PAIR_PENALTY_7=0.0597
PRIMER_LEFT_7_PENALTY=0.045432
PRIMER_RIGHT_7_PENALTY=0.014220
PRIMER_LEFT_7_SEQUENCE=TCCTGGTCTTACTGGATGTCACT
PRIMER_RIGHT_7_SEQUENCE=TTGAGCCATCCACTCATTTACTT
PRIMER_LEFT_7=666,23
PRIMER_RIGHT_7=1351,23
PRIMER_LEFT_7_TM=60.045
PRIMER_RIGHT_7_TM=60.014
PRIMER_LEFT_7_GC_PERCENT=47.826
PRIMER_RIGHT_7_GC_PERCENT=39.130
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_MISPRIMING_SCORE=12.00, CER Human D22Z3 repetitive DNA (centromeric DNA)
PRIMER_RIGHT_7_MISPRIMING_SCORE=12.00, L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_PAIR_7_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_7_END_STABILITY=6.4000
PRIMER_RIGHT_7_END_STABILITY=5.7000
PRIMER_PAIR_7_COMPL_ANY=6.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_7=686
PRIMER_PAIR_PENALTY_8=0.0651
PRIMER_LEFT_8_PENALTY=0.045432
PRIMER_RIGHT_8_PENALTY=0.019662
PRIMER_LEFT_8_SEQUENCE=TCCTGGTCTTACTGGATGTCACT
PRIMER_RIGHT_8_SEQUENCE=TCGCCTTTCTCCAAAATAATACA
PRIMER_LEFT_8=666,23
PRIMER_RIGHT_8=1486,23
PRIMER_LEFT_8_TM=60.045
PRIMER_RIGHT_8_TM=59.980
PRIMER_LEFT_8_GC_PERCENT=47.826
PRIMER_RIGHT_8_GC_PERCENT=34.783
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_MISPRIMING_SCORE=12.00, CER Human D22Z3 repetitive DNA (centromeric DNA)
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_8_MISPRIMING_SCORE=23.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_8_END_STABILITY=6.4000
PRIMER_RIGHT_8_END_STABILITY=5.6000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_8=821
PRIMER_PAIR_PENALTY_9=0.0660
PRIMER_LEFT_9_PENALTY=0.045432
PRIMER_RIGHT_9_PENALTY=0.020535
PRIMER_LEFT_9_SEQUENCE=TCCTGGTCTTACTGGATGTCACT
PRIMER_RIGHT_9_SEQUENCE=ATCTTGAGCCATCCACTCATTTA
PRIMER_LEFT_9=666,23
PRIMER_RIGHT_9=1354,23
PRIMER_LEFT_9_TM=60.045
PRIMER_RIGHT_9_TM=59.979
PRIMER_LEFT_9_GC_PERCENT=47.826
PRIMER_RIGHT_9_GC_PERCENT=39.130
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=2.00
PRIMER_LEFT_9_MISPRIMING_SCORE=12.00, CER Human D22Z3 repetitive DNA (centromeric DNA)
PRIMER_RIGHT_9_MISPRIMING_SCORE=11.00, L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence
PRIMER_PAIR_9_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_9_END_STABILITY=6.4000
PRIMER_RIGHT_9_END_STABILITY=6.2000
PRIMER_PAIR_9_COMPL_ANY=6.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_9=689
PRIMER_PAIR_PENALTY_10=0.0949
PRIMER_LEFT_10_PENALTY=0.049500
PRIMER_RIGHT_10_PENALTY=0.045432
PRIMER_LEFT_10_SEQUENCE=CAGTTTCTGACCCAACAGTTACC
PRIMER_RIGHT_10_SEQUENCE=AGTGACATCCAGTAAGACCAGGA
PRIMER_LEFT_10=65,23
PRIMER_RIGHT_10=688,23
PRIMER_LEFT_10_TM=59.950
PRIMER_RIGHT_10_TM=60.045
PRIMER_LEFT_10_GC_PERCENT=47.826
PRIMER_RIGHT_10_GC_PERCENT=47.826
PRIMER_LEFT_10_SELF_ANY=5.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=2.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_MISPRIMING_SCORE=12.00, reverse MER39 a consensus
PRIMER_RIGHT_10_MISPRIMING_SCORE=12.00, reverse PTR5 Human PTR5 mRNA for repetitive sequence
PRIMER_PAIR_10_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_10_END_STABILITY=7.2000
PRIMER_RIGHT_10_END_STABILITY=8.2000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_10=624
PRIMER_PAIR_PENALTY_11=0.0958
PRIMER_LEFT_11_PENALTY=0.020535
PRIMER_RIGHT_11_PENALTY=0.075241
PRIMER_LEFT_11_SEQUENCE=GCTATGTCATTTCCATCTTCTGG
PRIMER_RIGHT_11_SEQUENCE=ACAGTGAATCGCTACGATGTTTT
PRIMER_LEFT_11=1109,23
PRIMER_RIGHT_11=1381,23
PRIMER_LEFT_11_TM=59.979
PRIMER_RIGHT_11_TM=60.075
PRIMER_LEFT_11_GC_PERCENT=43.478
PRIMER_RIGHT_11_GC_PERCENT=39.130
PRIMER_LEFT_11_SELF_ANY=5.00
PRIMER_RIGHT_11_SELF_ANY=6.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_MISPRIMING_SCORE=12.00, reverse Tigger2 Autonomous DNA transposon
PRIMER_RIGHT_11_MISPRIMING_SCORE=11.00, MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_PAIR_11_MISPRIMING_SCORE=21.00, MER21B a consensus
PRIMER_LEFT_11_END_STABILITY=8.2000
PRIMER_RIGHT_11_END_STABILITY=7.0000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_11=273
PRIMER_PAIR_PENALTY_12=0.1027
PRIMER_LEFT_12_PENALTY=0.088604
PRIMER_RIGHT_12_PENALTY=0.014092
PRIMER_LEFT_12_SEQUENCE=ACTTCTGGTTGCACACATCTTTT
PRIMER_RIGHT_12_SEQUENCE=GATATATGAACATGTGGCAGGGT
PRIMER_LEFT_12=1851,23
PRIMER_RIGHT_12=2092,23
PRIMER_LEFT_12_TM=60.089
PRIMER_RIGHT_12_TM=59.986
PRIMER_LEFT_12_GC_PERCENT=39.130
PRIMER_RIGHT_12_GC_PERCENT=43.478
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=6.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_MISPRIMING_SCORE=12.00, MER42a MER42 repetitive sequence: subfamily MER42a - a consensus
PRIMER_RIGHT_12_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_12_MISPRIMING_SCORE=23.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_12_END_STABILITY=7.3000
PRIMER_RIGHT_12_END_STABILITY=9.1000
PRIMER_PAIR_12_COMPL_ANY=7.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_12=242
PRIMER_PAIR_PENALTY_13=0.1028
PRIMER_LEFT_13_PENALTY=0.014220
PRIMER_RIGHT_13_PENALTY=0.088604
PRIMER_LEFT_13_SEQUENCE=AAGTAAATGAGTGGATGGCTCAA
PRIMER_RIGHT_13_SEQUENCE=AAAAGATGTGTGCAACCAGAAGT
PRIMER_LEFT_13=1329,23
PRIMER_RIGHT_13=1873,23
PRIMER_LEFT_13_TM=60.014
PRIMER_RIGHT_13_TM=60.089
PRIMER_LEFT_13_GC_PERCENT=39.130
PRIMER_RIGHT_13_GC_PERCENT=39.130
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_MISPRIMING_SCORE=12.00, L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_RIGHT_13_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_13_MISPRIMING_SCORE=24.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_13_END_STABILITY=7.0000
PRIMER_RIGHT_13_END_STABILITY=6.4000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_13=545
PRIMER_PAIR_PENALTY_14=0.1060
PRIMER_LEFT_14_PENALTY=0.091943
PRIMER_RIGHT_14_PENALTY=0.014092
PRIMER_LEFT_14_SEQUENCE=ATCTTCTGGAAGGCAGTCTTTCT
PRIMER_RIGHT_14_SEQUENCE=GATATATGAACATGTGGCAGGGT
PRIMER_LEFT_14=1123,23
PRIMER_RIGHT_14=2092,23
PRIMER_LEFT_14_TM=59.908
PRIMER_RIGHT_14_TM=59.986
PRIMER_LEFT_14_GC_PERCENT=43.478
PRIMER_RIGHT_14_GC_PERCENT=43.478
PRIMER_LEFT_14_SELF_ANY=6.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=3.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_MISPRIMING_SCORE=12.00, LTR4 LTR from human endogenous retrovirus ERV3, POL-ENV-3'LTR region
PRIMER_RIGHT_14_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_14_MISPRIMING_SCORE=24.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_14_END_STABILITY=7.0000
PRIMER_RIGHT_14_END_STABILITY=9.1000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_14=970
PRIMER_PAIR_PENALTY_15=0.1062
PRIMER_LEFT_15_PENALTY=0.091943
PRIMER_RIGHT_15_PENALTY=0.014220
PRIMER_LEFT_15_SEQUENCE=ATCTTCTGGAAGGCAGTCTTTCT
PRIMER_RIGHT_15_SEQUENCE=TTGAGCCATCCACTCATTTACTT
PRIMER_LEFT_15=1123,23
PRIMER_RIGHT_15=1351,23
PRIMER_LEFT_15_TM=59.908
PRIMER_RIGHT_15_TM=60.014
PRIMER_LEFT_15_GC_PERCENT=43.478
PRIMER_RIGHT_15_GC_PERCENT=39.130
PRIMER_LEFT_15_SELF_ANY=6.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=3.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_MISPRIMING_SCORE=12.00, LTR4 LTR from human endogenous retrovirus ERV3, POL-ENV-3'LTR region
PRIMER_RIGHT_15_MISPRIMING_SCORE=12.00, L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_PAIR_15_MISPRIMING_SCORE=22.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_15_END_STABILITY=7.0000
PRIMER_RIGHT_15_END_STABILITY=5.7000
PRIMER_PAIR_15_COMPL_ANY=6.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_15=229
PRIMER_PAIR_PENALTY_16=0.1083
PRIMER_LEFT_16_PENALTY=0.019662
PRIMER_RIGHT_16_PENALTY=0.088604
PRIMER_LEFT_16_SEQUENCE=TGTATTATTTTGGAGAAAGGCGA
PRIMER_RIGHT_16_SEQUENCE=AAAAGATGTGTGCAACCAGAAGT
PRIMER_LEFT_16=1464,23
PRIMER_RIGHT_16=1873,23
PRIMER_LEFT_16_TM=59.980
PRIMER_RIGHT_16_TM=60.089
PRIMER_LEFT_16_GC_PERCENT=34.783
PRIMER_RIGHT_16_GC_PERCENT=39.130
PRIMER_LEFT_16_SELF_ANY=3.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_MISPRIMING_SCORE=12.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_16_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_16_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_16_END_STABILITY=11.4000
PRIMER_RIGHT_16_END_STABILITY=6.4000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_16=410
PRIMER_PAIR_PENALTY_17=0.1084
PRIMER_LEFT_17_PENALTY=0.094334
PRIMER_RIGHT_17_PENALTY=0.014092
PRIMER_LEFT_17_SEQUENCE=TTGATGTATTCGACGGTCTACCT
PRIMER_RIGHT_17_SEQUENCE=GATATATGAACATGTGGCAGGGT
PRIMER_LEFT_17=1758,23
PRIMER_RIGHT_17=2092,23
PRIMER_LEFT_17_TM=59.906
PRIMER_RIGHT_17_TM=59.986
PRIMER_LEFT_17_GC_PERCENT=43.478
PRIMER_RIGHT_17_GC_PERCENT=43.478
PRIMER_LEFT_17_SELF_ANY=5.00
PRIMER_RIGHT_17_SELF_ANY=6.00
PRIMER_LEFT_17_SELF_END=2.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_MISPRIMING_SCORE=11.00, SVA Composite retroposon
PRIMER_RIGHT_17_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_17_MISPRIMING_SCORE=21.00, SVA Composite retroposon
PRIMER_LEFT_17_END_STABILITY=6.9000
PRIMER_RIGHT_17_END_STABILITY=9.1000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_17=335
PRIMER_PAIR_PENALTY_18=0.1086
PRIMER_LEFT_18_PENALTY=0.014220
PRIMER_RIGHT_18_PENALTY=0.094334
PRIMER_LEFT_18_SEQUENCE=AAGTAAATGAGTGGATGGCTCAA
PRIMER_RIGHT_18_SEQUENCE=AGGTAGACCGTCGAATACATCAA
PRIMER_LEFT_18=1329,23
PRIMER_RIGHT_18=1780,23
PRIMER_LEFT_18_TM=60.014
PRIMER_RIGHT_18_TM=59.906
PRIMER_LEFT_18_GC_PERCENT=39.130
PRIMER_RIGHT_18_GC_PERCENT=43.478
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=5.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_MISPRIMING_SCORE=12.00, L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_RIGHT_18_MISPRIMING_SCORE=11.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_18_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_18_END_STABILITY=7.0000
PRIMER_RIGHT_18_END_STABILITY=6.9000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_18=452
PRIMER_PAIR_PENALTY_19=0.1116
PRIMER_LEFT_19_PENALTY=0.091943
PRIMER_RIGHT_19_PENALTY=0.019662
PRIMER_LEFT_19_SEQUENCE=ATCTTCTGGAAGGCAGTCTTTCT
PRIMER_RIGHT_19_SEQUENCE=TCGCCTTTCTCCAAAATAATACA
PRIMER_LEFT_19=1123,23
PRIMER_RIGHT_19=1486,23
PRIMER_LEFT_19_TM=59.908
PRIMER_RIGHT_19_TM=59.980
PRIMER_LEFT_19_GC_PERCENT=43.478
PRIMER_RIGHT_19_GC_PERCENT=34.783
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=3.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_MISPRIMING_SCORE=12.00, LTR4 LTR from human endogenous retrovirus ERV3, POL-ENV-3'LTR region
PRIMER_RIGHT_19_MISPRIMING_SCORE=12.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_19_MISPRIMING_SCORE=22.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_19_END_STABILITY=7.0000
PRIMER_RIGHT_19_END_STABILITY=5.6000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_19=364
=