PRIMER_SEQUENCE_ID=NM_014369
SEQUENCE=CCGCGGCGTCCACACTCGCCGCGCGCGCGGGGCCGGGCTGGACCTTGCTGGCCCGCGGCGCCATGAGCCGCAGCCTGGACTCGGCGCGGAGCTTCCTGGAGCGGCTGGAAGCGCGGGGCGGCCGGGAGGGGGCAGTCCTCGCCGGCGAGTTCAGCGACATCCAGGCCTGCTCGGCCGCCTGGAAGGCTGACGGCGTGTGCTCCACCGTGGCCGGCAGTCGGCCAGAGAACGTGAGGAAGAACCGCTACAAAGACGTGCTGCCTTATGATCAGACGCGAGTAATCCTCTCCCTGCTCCAGGAAGAGGGACACAGCGACTACATTAATGGCAACTTCATCCGGGGCGTGGATGGAAGCCTGGCCTACATTGCCACGCAAGGACCCTTGCCTCACACCCTGCTAGACTTCTGGAGACTGGTCTGGGAGTTTGGGGTCAAGGTGATCCTGATGGCCTGTCGAGAGATAGAGAATGGGCGGAAAAGGTGTGAGCGGTACTGGGCCCAGGAGCAGGAGCCACTGCAGACTGGGCTTTTCTGCATCACTCTGATAAAGGAGAAGTGGCTGAATGAGGACATCATGCTCAGGACCCTCAAGGTCACATTCCAGAAGGAGTCCCGTTCTGTGTACCAGCTACAGTATATGTCCTGGCCAGACCGTGGGGTCCCCAGCAGTCCTGACCACATGCTCGCCATGGTGGAGGAAGCCCGTCGCCTCCAGGGATCTGGCCCTGAACCCCTCTGTGTCCACTGCAGTGCGGGTTGTGGGCGAACAGGCGTCCTGTGCACCGTGGATTATGTGAGGCAGCTGCTCCTGACCCAGATGATCCCACCTGACTTCAGTCTCTTTGATGTGGTCCTTAAGATGAGGAAGCAGCGGCCTGCGGCCGTGCAGACAGAGGAGCAGTACAGGTTCCTGTACCACACGGTGGCTCAGATGTTCTGCTCCACACTCCAGAATGCCAGCCCCCACTACCAGAACATCAAAGAGAATTGTGCCCCACTCTACGACGATGCCCTCTTCCTCCGGACTCCCCAGGCACTTCTCGCCATACCCCGCCCACCAGGAGGGGTCCTCAGGAGCATCTCTGTGCCCGGGTCCCCGGGCCACGCCATGGCTGACACCTACGCGGTGGTGCAGAAGCGCGGGGCTCCAGCGGGCGCCGGGAGTGGGACGCAGACGGGGACGGGGACGGGGACGGGGGCGCGCAGCGCGGAGGAGGCGCCGCTCTACAGCAAGGTGACGCCGCGCGCCCAGCGACCCGGGGCGCACGCGGAGGACGCGAGGGGGACGCTGCCTGGCCGCGTTCCTGCTGACCAAAGTCCTGCCGGATCTGGCGCCTACGAGGACGTGGCGGGTGGAGCTCAGACCGGTGGGCTAGGTTTCAACCTGCGCATTGGGAGGCCGAAGGGTCCCCGGGACCCGCCTGCTGAGTGGACCCGGGTGTAAGTCTAACGCCAGTTCCTGCCTGTTGCCTCTTGTGAGCTCGGACTGCTGATGCCCCGGTGCTGCTGAGCGCCGTGCGCAGAATGGAAACAGTGGGCCTGGATCAAAGTTAAAGTTTCTCAGGGTGGGAAATGTGGGGGCTTTGCCCAATGACTGTAGCATTCAAGGCTTGAGGCTGGAGGAGGTAGCTAGGGTATAGTGGCTGGTGAGGCTGCACAGAGCAGATTCAAGAAAGAAGATCAGGAAGGGGCATGACCCCTGAGTTATGAAGGGGAGAAGGGACAGATGAGCTTCCGGAGACTGCTCTCCTCACCACACAGCACTAGTCCATCCTCAGCACCTGAGCCTCCCTCACTTGGACACTCAGGGGACCACACAGAGAAGTGGATGGACACTTCGCCATCCAGGCAGAACTAAGCCAGGCATAACCACAGCCAAGCAGATTAACCCCAGGCAGACCGATAAAAAGACCTCCAGATAGGCAGACAGACAGATGGACCACCAACCTGGACAGACAGCCAAAGCTTCAGAGATACAGTCCACAGGTGGACAAAGGGATCCCCAGCCAGAGAGAGAGAGACCAGCCAACAGCTTGATAGACCAGTGCAGCCAGAGAGACCACCAAACAGAGCCCCCAAAAGACAGACATCTCTGCTAGCTGGACAGCCAGGTGGACCCCCTAAGTTAGTCAGATTACTAGACAGATATAAACAGATCCCCTGCTGAACAGATATACAGAGTTCTCAGACCCCACCCCACCCTCAGGTGGGCTGGCTGGCTGACAGACCTTCTGGCCAGACAGACTCCTAACCAACCAGATGGACGCCAGACAGGCAGACATCAGTCCACATGGAATCCTGACATCCCAGCCAGCCGGCCAGACTCTCATCTTGATGTCTTGATGGATGGACCCCAGCTAGTCAGACATGATCCTCCAGATTGACAGACAAGTCCCCCAAATGAGTACACATCTCCAGCTATTCAGACAGATGGACCCCCAGCAAATCAGGACCTATCTAGGCAGACCCCAGCCAGACCCCCGCCAGACAGACTCCCAACCAGACTGACCCCTTACTATTCACACAGCCTGCCGAGTAGCTGGGACTACAGGTCTAATTTTTTTTTTTTTTAAGAAATGAGTTTTTGCCATGTTGCCCAGACTGGTCTTGAACTCCCAACCTCAAGCAATCCTCCTGCCTCAGCCTCCCAAAGTGCTGAGATTACAGGTGTGAGCCACCAGGCTCAGCCCCCTAAGATTTGAAACACTTTAAATGGCCCATGGTAGGGTTCCTGCTAGGATAAAACATTAAGCGGCTGTTAAAAGAAATAAAAGGAGGACACGTCTCTGTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 32793, GC content failed 2308, low tm 8824, high tm 15408, high any compl 5, high end compl 40, high repeat similarity 39, long poly-x seq 12, ok 6157
PRIMER_RIGHT_EXPLAIN=considered 32641, GC content failed 2227, low tm 9111, high tm 14942, high any compl 1, high end compl 9, high repeat similarity 11, long poly-x seq 34, ok 6306
PRIMER_PAIR_EXPLAIN=considered 1082, unacceptable product size 1012, high end compl 6, ok 64
PRIMER_PAIR_PENALTY=0.0830
PRIMER_LEFT_PENALTY=0.042816
PRIMER_RIGHT_PENALTY=0.040223
PRIMER_LEFT_SEQUENCE=GCGACTACATTAATGGCAACTTC
PRIMER_RIGHT_SEQUENCE=CTCTTTGATGTTCTGGTAGTGGG
PRIMER_LEFT=312,23
PRIMER_RIGHT=985,23
PRIMER_LEFT_TM=60.043
PRIMER_RIGHT_TM=60.040
PRIMER_LEFT_GC_PERCENT=43.478
PRIMER_RIGHT_GC_PERCENT=47.826
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_MISPRIMING_SCORE=11.00, reverse Tigger2 Autonomous DNA transposon
PRIMER_RIGHT_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=674
PRIMER_PAIR_PENALTY_1=0.0841
PRIMER_LEFT_1_PENALTY=0.042816
PRIMER_RIGHT_1_PENALTY=0.041315
PRIMER_LEFT_1_SEQUENCE=GCGACTACATTAATGGCAACTTC
PRIMER_RIGHT_1_SEQUENCE=AGTCTCCAGAAGTCTAGCAGGGT
PRIMER_LEFT_1=312,23
PRIMER_RIGHT_1=414,23
PRIMER_LEFT_1_TM=60.043
PRIMER_RIGHT_1_TM=59.959
PRIMER_LEFT_1_GC_PERCENT=43.478
PRIMER_RIGHT_1_GC_PERCENT=52.174
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_MISPRIMING_SCORE=11.00, reverse Tigger2 Autonomous DNA transposon
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_1_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=9.1000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=103
PRIMER_PAIR_PENALTY_2=0.0994
PRIMER_LEFT_2_PENALTY=0.010990
PRIMER_RIGHT_2_PENALTY=0.088390
PRIMER_LEFT_2_SEQUENCE=CAGACAGAGGAGCAGTACAGGTT
PRIMER_RIGHT_2_SEQUENCE=AGTGTCCATCCACTTCTCTGTGT
PRIMER_LEFT_2=887,23
PRIMER_RIGHT_2=1837,23
PRIMER_LEFT_2_TM=59.989
PRIMER_RIGHT_2_TM=60.088
PRIMER_LEFT_2_GC_PERCENT=52.174
PRIMER_RIGHT_2_GC_PERCENT=47.826
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_MISPRIMING_SCORE=11.00, reverse MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_RIGHT_2_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_2_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=951
PRIMER_PAIR_PENALTY_3=0.1164
PRIMER_LEFT_3_PENALTY=0.076178
PRIMER_RIGHT_3_PENALTY=0.040223
PRIMER_LEFT_3_SEQUENCE=TAGACTTCTGGAGACTGGTCTGG
PRIMER_RIGHT_3_SEQUENCE=CTCTTTGATGTTCTGGTAGTGGG
PRIMER_LEFT_3=399,23
PRIMER_RIGHT_3=985,23
PRIMER_LEFT_3_TM=59.924
PRIMER_RIGHT_3_TM=60.040
PRIMER_LEFT_3_GC_PERCENT=52.174
PRIMER_RIGHT_3_GC_PERCENT=47.826
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_3_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=587
PRIMER_PAIR_PENALTY_4=0.1190
PRIMER_LEFT_4_PENALTY=0.042816
PRIMER_RIGHT_4_PENALTY=0.076178
PRIMER_LEFT_4_SEQUENCE=GCGACTACATTAATGGCAACTTC
PRIMER_RIGHT_4_SEQUENCE=CCAGACCAGTCTCCAGAAGTCTA
PRIMER_LEFT_4=312,23
PRIMER_RIGHT_4=421,23
PRIMER_LEFT_4_TM=60.043
PRIMER_RIGHT_4_TM=59.924
PRIMER_LEFT_4_GC_PERCENT=43.478
PRIMER_RIGHT_4_GC_PERCENT=52.174
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_MISPRIMING_SCORE=11.00, reverse Tigger2 Autonomous DNA transposon
PRIMER_RIGHT_4_MISPRIMING_SCORE=11.00, L1PB3 3'-end of L1 repeat (subfamily L1PB3) - a consensus sequence
PRIMER_PAIR_4_MISPRIMING_SCORE=22.00, reverse Tigger2 Autonomous DNA transposon
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=5.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=110
PRIMER_PAIR_PENALTY_5=0.1666
PRIMER_LEFT_5_PENALTY=0.078208
PRIMER_RIGHT_5_PENALTY=0.088390
PRIMER_LEFT_5_SEQUENCE=AAGTTAAAGTTTCTCAGGGTGGG
PRIMER_RIGHT_5_SEQUENCE=AGTGTCCATCCACTTCTCTGTGT
PRIMER_LEFT_5=1548,23
PRIMER_RIGHT_5=1837,23
PRIMER_LEFT_5_TM=59.922
PRIMER_RIGHT_5_TM=60.088
PRIMER_LEFT_5_GC_PERCENT=43.478
PRIMER_RIGHT_5_GC_PERCENT=47.826
PRIMER_LEFT_5_SELF_ANY=5.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_MISPRIMING_SCORE=12.00, reverse MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_5_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_5_MISPRIMING_SCORE=21.00, reverse MER25 Older LINE1 5' end region - a consensus
PRIMER_LEFT_5_END_STABILITY=9.4000
PRIMER_RIGHT_5_END_STABILITY=6.4000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_5=290
PRIMER_PAIR_PENALTY_6=0.1822
PRIMER_LEFT_6_PENALTY=0.040223
PRIMER_RIGHT_6_PENALTY=0.141958
PRIMER_LEFT_6_SEQUENCE=CCCACTACCAGAACATCAAAGAG
PRIMER_RIGHT_6_SEQUENCE=GTCTGCCTATCTGGAGGTCTTTT
PRIMER_LEFT_6=963,23
PRIMER_RIGHT_6=1928,23
PRIMER_LEFT_6_TM=60.040
PRIMER_RIGHT_6_TM=60.142
PRIMER_LEFT_6_GC_PERCENT=47.826
PRIMER_RIGHT_6_GC_PERCENT=47.826
PRIMER_LEFT_6_SELF_ANY=2.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_6_MISPRIMING_SCORE=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_PAIR_6_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_6_END_STABILITY=6.7000
PRIMER_RIGHT_6_END_STABILITY=7.3000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_6=966
PRIMER_PAIR_PENALTY_7=0.2059
PRIMER_LEFT_7_PENALTY=0.078208
PRIMER_RIGHT_7_PENALTY=0.127701
PRIMER_LEFT_7_SEQUENCE=AAGTTAAAGTTTCTCAGGGTGGG
PRIMER_RIGHT_7_SEQUENCE=GCCTAGATAGGTCCTGATTTGCT
PRIMER_LEFT_7=1548,23
PRIMER_RIGHT_7=2463,23
PRIMER_LEFT_7_TM=59.922
PRIMER_RIGHT_7_TM=60.128
PRIMER_LEFT_7_GC_PERCENT=43.478
PRIMER_RIGHT_7_GC_PERCENT=47.826
PRIMER_LEFT_7_SELF_ANY=5.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_MISPRIMING_SCORE=12.00, reverse MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_7_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_7_MISPRIMING_SCORE=20.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_7_END_STABILITY=9.4000
PRIMER_RIGHT_7_END_STABILITY=8.5000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_7=916
PRIMER_PAIR_PENALTY_8=0.2079
PRIMER_LEFT_8_PENALTY=0.088390
PRIMER_RIGHT_8_PENALTY=0.119476
PRIMER_LEFT_8_SEQUENCE=ACACAGAGAAGTGGATGGACACT
PRIMER_RIGHT_8_SEQUENCE=TTTATCCTAGCAGGAACCCTACC
PRIMER_LEFT_8=1815,23
PRIMER_RIGHT_8=2744,23
PRIMER_LEFT_8_TM=60.088
PRIMER_RIGHT_8_TM=59.881
PRIMER_LEFT_8_GC_PERCENT=47.826
PRIMER_RIGHT_8_GC_PERCENT=47.826
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=2.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_MISPRIMING_SCORE=11.00, reverse L1MA2 3'-end of L1 repeat (subfamily L1MA2) - a consensus sequence
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_8_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_8_END_STABILITY=6.1000
PRIMER_RIGHT_8_END_STABILITY=6.9000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_8=930
PRIMER_PAIR_PENALTY_9=0.2082
PRIMER_LEFT_9_PENALTY=0.078208
PRIMER_RIGHT_9_PENALTY=0.130037
PRIMER_LEFT_9_SEQUENCE=AAGTTAAAGTTTCTCAGGGTGGG
PRIMER_RIGHT_9_SEQUENCE=TGTATATCTGTTCAGCAGGGGAT
PRIMER_LEFT_9=1548,23
PRIMER_RIGHT_9=2178,23
PRIMER_LEFT_9_TM=59.922
PRIMER_RIGHT_9_TM=59.870
PRIMER_LEFT_9_GC_PERCENT=43.478
PRIMER_RIGHT_9_GC_PERCENT=43.478
PRIMER_LEFT_9_SELF_ANY=5.00
PRIMER_RIGHT_9_SELF_ANY=5.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=2.00
PRIMER_LEFT_9_MISPRIMING_SCORE=12.00, reverse MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_PAIR_9_MISPRIMING_SCORE=20.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_9_END_STABILITY=9.4000
PRIMER_RIGHT_9_END_STABILITY=9.3000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_9=631
PRIMER_PAIR_PENALTY_10=0.2161
PRIMER_LEFT_10_PENALTY=0.088390
PRIMER_RIGHT_10_PENALTY=0.127701
PRIMER_LEFT_10_SEQUENCE=ACACAGAGAAGTGGATGGACACT
PRIMER_RIGHT_10_SEQUENCE=GCCTAGATAGGTCCTGATTTGCT
PRIMER_LEFT_10=1815,23
PRIMER_RIGHT_10=2463,23
PRIMER_LEFT_10_TM=60.088
PRIMER_RIGHT_10_TM=60.128
PRIMER_LEFT_10_GC_PERCENT=47.826
PRIMER_RIGHT_10_GC_PERCENT=47.826
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=2.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_MISPRIMING_SCORE=11.00, reverse L1MA2 3'-end of L1 repeat (subfamily L1MA2) - a consensus sequence
PRIMER_RIGHT_10_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_10_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_10_END_STABILITY=6.1000
PRIMER_RIGHT_10_END_STABILITY=8.5000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_10=649
PRIMER_PAIR_PENALTY_11=0.2184
PRIMER_LEFT_11_PENALTY=0.088390
PRIMER_RIGHT_11_PENALTY=0.130037
PRIMER_LEFT_11_SEQUENCE=ACACAGAGAAGTGGATGGACACT
PRIMER_RIGHT_11_SEQUENCE=TGTATATCTGTTCAGCAGGGGAT
PRIMER_LEFT_11=1815,23
PRIMER_RIGHT_11=2178,23
PRIMER_LEFT_11_TM=60.088
PRIMER_RIGHT_11_TM=59.870
PRIMER_LEFT_11_GC_PERCENT=47.826
PRIMER_RIGHT_11_GC_PERCENT=43.478
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=5.00
PRIMER_LEFT_11_SELF_END=2.00
PRIMER_RIGHT_11_SELF_END=2.00
PRIMER_LEFT_11_MISPRIMING_SCORE=11.00, reverse L1MA2 3'-end of L1 repeat (subfamily L1MA2) - a consensus sequence
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_PAIR_11_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_11_END_STABILITY=6.1000
PRIMER_RIGHT_11_END_STABILITY=9.3000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_11=364
PRIMER_PAIR_PENALTY_12=0.2202
PRIMER_LEFT_12_PENALTY=0.078208
PRIMER_RIGHT_12_PENALTY=0.141958
PRIMER_LEFT_12_SEQUENCE=AAGTTAAAGTTTCTCAGGGTGGG
PRIMER_RIGHT_12_SEQUENCE=GTCTGCCTATCTGGAGGTCTTTT
PRIMER_LEFT_12=1548,23
PRIMER_RIGHT_12=1928,23
PRIMER_LEFT_12_TM=59.922
PRIMER_RIGHT_12_TM=60.142
PRIMER_LEFT_12_GC_PERCENT=43.478
PRIMER_RIGHT_12_GC_PERCENT=47.826
PRIMER_LEFT_12_SELF_ANY=5.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_MISPRIMING_SCORE=12.00, reverse MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_12_MISPRIMING_SCORE=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_PAIR_12_MISPRIMING_SCORE=20.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_12_END_STABILITY=9.4000
PRIMER_RIGHT_12_END_STABILITY=7.3000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_12=381
PRIMER_PAIR_PENALTY_13=0.2303
PRIMER_LEFT_13_PENALTY=0.088390
PRIMER_RIGHT_13_PENALTY=0.141958
PRIMER_LEFT_13_SEQUENCE=ACACAGAGAAGTGGATGGACACT
PRIMER_RIGHT_13_SEQUENCE=GTCTGCCTATCTGGAGGTCTTTT
PRIMER_LEFT_13=1815,23
PRIMER_RIGHT_13=1928,23
PRIMER_LEFT_13_TM=60.088
PRIMER_RIGHT_13_TM=60.142
PRIMER_LEFT_13_GC_PERCENT=47.826
PRIMER_RIGHT_13_GC_PERCENT=47.826
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=2.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_MISPRIMING_SCORE=11.00, reverse L1MA2 3'-end of L1 repeat (subfamily L1MA2) - a consensus sequence
PRIMER_RIGHT_13_MISPRIMING_SCORE=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_PAIR_13_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_13_END_STABILITY=6.1000
PRIMER_RIGHT_13_END_STABILITY=7.3000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_13=114
PRIMER_PAIR_PENALTY_14=0.2441
PRIMER_LEFT_14_PENALTY=0.124598
PRIMER_RIGHT_14_PENALTY=0.119476
PRIMER_LEFT_14_SEQUENCE=GATCCTCCAGATTGACAGACAAG
PRIMER_RIGHT_14_SEQUENCE=TTTATCCTAGCAGGAACCCTACC
PRIMER_LEFT_14=2370,23
PRIMER_RIGHT_14=2744,23
PRIMER_LEFT_14_TM=60.125
PRIMER_RIGHT_14_TM=59.881
PRIMER_LEFT_14_GC_PERCENT=47.826
PRIMER_RIGHT_14_GC_PERCENT=47.826
PRIMER_LEFT_14_SELF_ANY=5.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=3.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_14_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_14_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_14_END_STABILITY=6.7000
PRIMER_RIGHT_14_END_STABILITY=6.9000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_14=375
PRIMER_PAIR_PENALTY_15=0.2495
PRIMER_LEFT_15_PENALTY=0.130037
PRIMER_RIGHT_15_PENALTY=0.119476
PRIMER_LEFT_15_SEQUENCE=ATCCCCTGCTGAACAGATATACA
PRIMER_RIGHT_15_SEQUENCE=TTTATCCTAGCAGGAACCCTACC
PRIMER_LEFT_15=2156,23
PRIMER_RIGHT_15=2744,23
PRIMER_LEFT_15_TM=59.870
PRIMER_RIGHT_15_TM=59.881
PRIMER_LEFT_15_GC_PERCENT=43.478
PRIMER_RIGHT_15_GC_PERCENT=47.826
PRIMER_LEFT_15_SELF_ANY=5.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=2.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_15_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_15_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_15_END_STABILITY=5.6000
PRIMER_RIGHT_15_END_STABILITY=6.9000
PRIMER_PAIR_15_COMPL_ANY=6.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_15=589
PRIMER_PAIR_PENALTY_16=0.2577
PRIMER_LEFT_16_PENALTY=0.040223
PRIMER_RIGHT_16_PENALTY=0.217466
PRIMER_LEFT_16_SEQUENCE=CCCACTACCAGAACATCAAAGAG
PRIMER_RIGHT_16_SEQUENCE=ATTTCCCACCCTGAGAAACTTTA
PRIMER_LEFT_16=963,23
PRIMER_RIGHT_16=1574,23
PRIMER_LEFT_16_TM=60.040
PRIMER_RIGHT_16_TM=60.217
PRIMER_LEFT_16_GC_PERCENT=47.826
PRIMER_RIGHT_16_GC_PERCENT=39.130
PRIMER_LEFT_16_SELF_ANY=2.00
PRIMER_RIGHT_16_SELF_ANY=7.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=3.00
PRIMER_LEFT_16_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_16_MISPRIMING_SCORE=11.00, reverse MSTc consensus sequence
PRIMER_PAIR_16_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_16_END_STABILITY=6.7000
PRIMER_RIGHT_16_END_STABILITY=6.3000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_16=612
PRIMER_PAIR_PENALTY_17=0.2577
PRIMER_LEFT_17_PENALTY=0.130037
PRIMER_RIGHT_17_PENALTY=0.127701
PRIMER_LEFT_17_SEQUENCE=ATCCCCTGCTGAACAGATATACA
PRIMER_RIGHT_17_SEQUENCE=GCCTAGATAGGTCCTGATTTGCT
PRIMER_LEFT_17=2156,23
PRIMER_RIGHT_17=2463,23
PRIMER_LEFT_17_TM=59.870
PRIMER_RIGHT_17_TM=60.128
PRIMER_LEFT_17_GC_PERCENT=43.478
PRIMER_RIGHT_17_GC_PERCENT=47.826
PRIMER_LEFT_17_SELF_ANY=5.00
PRIMER_RIGHT_17_SELF_ANY=6.00
PRIMER_LEFT_17_SELF_END=2.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_17_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_17_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_17_END_STABILITY=5.6000
PRIMER_RIGHT_17_END_STABILITY=8.5000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_17=308
PRIMER_PAIR_PENALTY_18=0.2599
PRIMER_LEFT_18_PENALTY=0.140413
PRIMER_RIGHT_18_PENALTY=0.119476
PRIMER_LEFT_18_SEQUENCE=GACCGATAAAAAGACCTCCAGAT
PRIMER_RIGHT_18_SEQUENCE=TTTATCCTAGCAGGAACCCTACC
PRIMER_LEFT_18=1898,23
PRIMER_RIGHT_18=2744,23
PRIMER_LEFT_18_TM=59.860
PRIMER_RIGHT_18_TM=59.881
PRIMER_LEFT_18_GC_PERCENT=43.478
PRIMER_RIGHT_18_GC_PERCENT=47.826
PRIMER_LEFT_18_SELF_ANY=2.00
PRIMER_RIGHT_18_SELF_ANY=6.00
PRIMER_LEFT_18_SELF_END=2.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_MISPRIMING_SCORE=11.00, MLT1a (MLT1a subfamily) - consensus sequence
PRIMER_RIGHT_18_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_18_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_18_END_STABILITY=6.6000
PRIMER_RIGHT_18_END_STABILITY=6.9000
PRIMER_PAIR_18_COMPL_ANY=6.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_18=847
PRIMER_PAIR_PENALTY_19=0.2614
PRIMER_LEFT_19_PENALTY=0.141958
PRIMER_RIGHT_19_PENALTY=0.119476
PRIMER_LEFT_19_SEQUENCE=AAAAGACCTCCAGATAGGCAGAC
PRIMER_RIGHT_19_SEQUENCE=TTTATCCTAGCAGGAACCCTACC
PRIMER_LEFT_19=1906,23
PRIMER_RIGHT_19=2744,23
PRIMER_LEFT_19_TM=60.142
PRIMER_RIGHT_19_TM=59.881
PRIMER_LEFT_19_GC_PERCENT=47.826
PRIMER_RIGHT_19_GC_PERCENT=47.826
PRIMER_LEFT_19_SELF_ANY=3.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_19_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_19_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_19_END_STABILITY=6.4000
PRIMER_RIGHT_19_END_STABILITY=6.9000
PRIMER_PAIR_19_COMPL_ANY=6.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_19=839
=