PRIMER_SEQUENCE_ID=NM_014805
SEQUENCE=GCTTGCGCGTTAGAGATCGCTGTCCGCTCTTCCTATTGGTTCGTTTTTAGGAGCTCGGGGAATACGAAATATCCAGCCAATAGGAGCAGAGATGCCGGAACCGGGCTTGTGTGCCTCTGCTGAGGTGATCTGGCGCAGAGCGGAGGAGGTGCTTGGCGCTTCTCAGGCTCCTCCTCTCCCCTTGCGGCCTTTCTAACGTTGGCCCTGCTCTTGTGGCCTCCCGCAGAATGTGGATGACGCCCAAAAGAAGCAAGATGGAAGTCGACGAGGCTCTAGTTTTCCGGCCCGAGTGGACCCAGCGTTATTTGGTGGTGGAGCCTCCGGAGGGCGATGGGGCCCTGTGCCTGGTCTGTCGCCGCCTCATCGTAGCTACCCGCGAACGCGACGTCAGGCGCCACTACGAGGCTGAGCACGAATACTACGAGCGGTATGTGGCGGACGGCGAGCGCGCGGCCCTGGTGGAGCGTCTGCGTCAGGGCGACTTGCCCGTGGCCTCCTTCACTCCTGAAGAGAGAGCTGCTCGTGCAGGCCTCGGGCTCTGCCGCCTCTTGGCCTTGAAGGGTCGCGGCTGGGGTGAGGGGGACTTTGTATACCAGTGCATGGAGGTGTTGCTGAGAGAGGTACTGCCCGAGCATGTAAGCGTCCTGCAAGGCGTTGACTTATCTCCAGATATCACAAGGCAGAGGATCCTGAGCATTGACAGGAATCTACGCAACCAGCTTTTTAACCGAGCCAGGGACTTTAAAGCCTATTCTCTTGCCTTGGACGACCAGGCTTTTGTGGCCTATGAGAACTACCTCCTGGTCTTTATCCGCGGTGTAGGCCCTGAGTTGGAGGTGCAAGAAGATCTTCTGACCATAATCAACCTGACTCATCATTTCAGTGTTGGTGCGCTCATGTCGGCAATCCTAGAGTCCCTGCAGACAGCAGGGCTTAGCTTGCAGAGAATGGTTGGACTGACCACGACCCATACTTTGAGGATGATTGGTGAGAACTCAGGACTCGTCTCATACATGAGAGAAAAGGCCGTAAGCCCCAACTGTTGGAATGTCATTCATTATTCAGGATTTCTTCACTTGGAACTGTTGAGCTCCTATGATGTAGATGTTAATCAGATCATAAATACCATATCCGAATGGATAGTTTTGATTAAGACCAGAGGCGTTAGGCGACCTGAATTTCAGACTTTACTAACGGAATCTGAATCAGAGCATGGTGAAAGGGTTAATGGACGATGTCTGAACAATTGGCTTAGGAGAGGGAAAACTTTAAAACTAATATTCTCTCTAAGAAAAGAAATGGAAGCGTTCTTGGTTTCAGTAGGGGCAACAACAGTCCACTTCTCAGACAAACAATGGCTTTGTGACTTTGGCTTCTTGGTGGACATTATGGAACACCTTCGAGAACTCAGTGAAGAATTACGAGTTAGTAAAGTCTTTGCTGCTGCTGCCTTTGACCATATTTGTACTTTCGAAGTTAAGCTGAATTTATTTCAAAGACATATTGAGGAAAAAAATCTAACAGACTTTCCTGCCCTCAGAGAAGTTGTTGATGAGCTAAAACAGCAAAATAAGGAAGATGAAAAAATATTTGATCCTGATAGGTATCAAATGGTGATCTGTCGTCTCCAAAAAGAATTTGAGAGACATTTTAAGGACCTCAGGTTCATTAAAAAGGACTTAGAACTTTTTTCAAATCCATTTAACTTTAAACCTGAATATGCACCTATTTCAGTGAGGGTGGAGCTAACAAAACTTCAGGCAAACACTAATCTTTGGAATGAATACAGAATCAAAGACTTGGGGCAGTTTTATGCTGGATTGTCTGCTGAATCCTACCCAATTATCAAAGGGGTTGCCTGTAAGGTGGCATCCTTGTTTGATAGTAACCAAATCTGTGAAAAGGCTTTTTCATATTTGACTCGAAACCAACACACTTTGAGTCAGCCATTAACAGATGAGCATCTCCAAGCCCTGTTTCGGGTTGCCACAACTGAAATGGAGCCCGGTTGGGATGACCTTGTGAGAGAAAGAAATGAATCTAATCCATAAGGCTTTGTAGTACAAGATTGAAAAACTCAACAAGAATTTAATTCTAAAAGCAAAAATTGGTTTGAGTTTTCAAGTTTACTAATTTGGATTGTGAGAAAGTACCAAGTACCAGCCGTCCAAACTGATCACAATTAAAATTCTGACAGTTGCCTTTTTTTTCATCTCAAATGGCAGCATGGGACTGAAACATGAGAATGCCACCTTTTTTAAAACTTAGTTTAGTGACAAAGTCATTGTCTTTTATGATATAGTTAATTTTAAAGAGATTTAGTATTAATGTGAGTTGAATTTGCAGTCTGTTTTTTAGGTGTTCTGAAGATAAATGCCAAAAATTTCAGCTCTTATTTTAATGGAGTGTTAAAATTCTGATTCATATAGTCTTAAATTATCAACTCCTTAAATGTGCTTTTGAACCAATTTGCAGAAGCTCACATAGCAAGTTCATAAGTTTCCAAAAAGGAAGCCCATACATAACAGTGGAGGTGTTTTGTCTAACCATCAAAATGTTTGAGACTTTTTTTTAAACATTTCTGAGTTCGAAGGTAATACTGACAGATTTCTTCCCTCTTCCCTCCCCATCACCCACCTCAGTGATAACACATTACTGATAGAGGAAGTCATTAGAATCATTTTTAAGTTTCAGATATAGGAGACTTCATGCAATTTGGAGATAAGACTAATTATTGGGGGTTTTCCTTGGATTTTTTTTTTAATAACTGGGGGCTATTTTATCAGCTTGCCTATTAAAGGACTATGGTAAGTATAGAATCTTAATGGTTGCCAGTTAGTAATTCTTTTTTTTTTTTTTTTTACTGTAGACACAAGTTTGGCCCTATCAAAAACGATGAGGAAAAAAGATTGCACTCCAGGATTAGGAGGTGTGAGATATTTTAGCTTTTTTGTCTTATCTGCGTGGGTATTGCTGCTTTATTTTAAAAAATCCTGCCTAAAGTAAACACTTTGTTTTAAAATGATACAGTATCAGATTTTGTTAGATGCTAGAAATGGATTTATTCTAAAATTTGGAACTGTCGTACACATTCTATATGTAAGATAGCACACAAGTAGAAATATTTAAAAGCAGTCTTATTCACAGATTGCAGTAATTCTGTATTTCTACTAAGATAATCTGCTTTGTGCCAAAACAGTAATTTCCAAACTTCTGTTCACCATGAAAAGGCAATCTTAAAGTTCATTATGTAAAACTAATTATAAACAGGACCCAATTTATATTCATAGATCCTCTCAAGTATTATACAATTTAAAAACTCTTGTTCCAAAGTCCTGTCTTAACTATTGAAACACCTTAATCTGTGGTTACTAATCCAGCAAATTCAAGGAACCAGGCTATGACTAAGAATTTAGGTGGAATTGATGTCTGGGCAATTAAAATAAATGGCATAAGAGCTTAAAAACCAAAGTTGTGCCAGTGGCTTTCAACTAGAGGCAGTAACCTGTCATTCCAGAGGATGCTGAGAAATGTGTAGGGGCACTTTTTTGGTTGTCATATTTACTAGGGGCTTCTGTTGGCATTTAAGCCTAAAGACACTCACCCCTGCAGTGCATGGGACAGCCTGGCACAATGAAGAATTAGCCCTCCCAAAATGTAGATTATTTTATTTCAAGGGATAGGGCAGATTACCATTAGAAGCAAAATTAAAAGTACAAGCTGGGCAAACTGACAGAATACTAGATAGGAGAGACTAATTCCAACCTTCTAAATTTGGCTAGTAAAGTGCAATAAAGGCATTGATAAGTTCTGTTAGCTCACCATAGCACTTGTAAATCAGGAATTAATAATTGAATCAGATTTAAGGGCTCTGTCCTGTTATACATATTTAAGGCAGAAAAAAAGTTACATGTCGATTAGGTACTTATCAAGAATGGTCAAGCTGAGATTTTGGTTAATAGAGTAAGCTTACATATCTAGAGAAACAACATAGTGGAAAACCGAAAAAAAAAAACAGAAAAATCTACCGGTAATTTCCCAATAGCTTTGAATATTCACAGCAGAGCTTTATTACTTGAGAGAAAGACTGGAAGACCTGAAAGCCACTTCTGCTTTCTAACCCCAGTTCCTTAAATATTGAAATCTTGTACATTTTGTGAAATTCCAGTATGTTTTGCTTAAGGTGTTAATAAAATTAGTTTGCATCATGTAGTCATTGAGTGAGGGGGAGATATAAGCCAAGGATTTTAAATTGACCCTTAGCTATAGAGAATTTGCTATAAGCTAGTCTTGTTTGTAAAAAAAAAAAAAAAAAAAGAAAAAGAAAAAAGTGTATTTTACTGTTTTCTGTATTAAGTAATTCTGTAACTGCATGGCAGTCTTTTTTTTTTTAAATAAATATAGTTGTTACTGGTCCTGTTGTAGCAGTGAATATAGTTAAAATACGTACATTAAAAAAAAAATTATTAGGTCCTTACCAGTTACTGTCCTATAGCTCATTCCTACTAGTTTTCTTGACAGATTTGTATTCCCAGTGTCCCGTATTGCCACTCAAATTGCTCTACTATGCTAAGTCCTTGTTAATAGTCTTACCCTCCTTGAAACACTTGAACACTTGATGACTTTAGCTTTGAGGAGATACCATCTCCAGGTGTGCTTTCTTAGTCTTTGCAGGCACCTCTTCCCTTCAATATCTGTTCTTCGTATTTTTAAAAAAATTTGTTTTAGACTGCCTTGTTCTGTGTCAGCTCGCTAGCTGATCTCATTTCCTTCCATGGTTTCCTTACCATTTATATGCAAATGACTGTCAGATTCATATCTCCTTTCTAGATCTTCCCTAATTGATGTATCTAATTGCTAACAAATGCTCTTTGCTGTCTCAGGCACTACATGTCATTGATCTTGCCCCCAATCCTGCTCCTCCTCTCATGTTTCCTCTTTGACTAAATGGCATTACCACTACCAACCATTCATTTGTCCTTTTTACCAATTCTCCAATGCTGCCATTTTAATTCAGGCCATCAACCTACCTAAATTATAGCAACAGCCTCCTTATTAGTCTCCCTGTTTTTTATTTTTATTCCTTTCTACACTACAACCAAATTGCTCCAAAAGACTTACTGATCATGTCACTGCATTGCTTTCACCATTGCTCTTAGGGTACAATACAAATTTATCTTCATCTTTAAGGTCTCAGTATGCCACTTCATCTAGGAAACCTTCATTGATGCCCTCTAGATTAGGTGCCCTTACTATCCATTCCCTATACACCCTGTTCTTTCCCAGACATACACTTGGCACACTTTATTGTTACTGCTTATTGATCACTGCTAGACTGTAAGCTTTGTAAGGGCAGGGACCATATAAGCCTTGTTCACTGTTATATCTCTAGTGCTTAGCACAATGCCTGGCATTTCAATAAATGTTTGGACAAACGAATATTTGTGTAGTGTTTTACAATTTTTGAAGCTCTTTCACAGTCTTATTTGACCTTCACAGTCATTCTGCCTTAGACTGTCCATTGGGTAACTTTTATCCACATATTACTAATTGAAAAATGAAGACAA
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 62811, GC content failed 3657, low tm 35516, high tm 11057, high any compl 6, high end compl 18, high repeat similarity 44, long poly-x seq 401, ok 12112
PRIMER_RIGHT_EXPLAIN=considered 62792, GC content failed 3715, low tm 35807, high tm 10782, high any compl 2, high end compl 11, high repeat similarity 20, long poly-x seq 399, ok 12056
PRIMER_PAIR_EXPLAIN=considered 1106, unacceptable product size 1045, high end compl 3, ok 58
PRIMER_PAIR_PENALTY=0.0364
PRIMER_LEFT_PENALTY=0.021648
PRIMER_RIGHT_PENALTY=0.014712
PRIMER_LEFT_SEQUENCE=TTACCCTCCTTGAAACACTTGAA
PRIMER_RIGHT_SEQUENCE=GATCAATGACATGTAGTGCCTGA
PRIMER_LEFT=4589,23
PRIMER_RIGHT=4901,23
PRIMER_LEFT_TM=60.022
PRIMER_RIGHT_TM=60.015
PRIMER_LEFT_GC_PERCENT=39.130
PRIMER_RIGHT_GC_PERCENT=43.478
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_MISPRIMING_SCORE=11.00, PTR5 Human PTR5 mRNA for repetitive sequence
PRIMER_RIGHT_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_END_STABILITY=7.3000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=313
PRIMER_PAIR_PENALTY_1=0.0464
PRIMER_LEFT_1_PENALTY=0.010990
PRIMER_RIGHT_1_PENALTY=0.035384
PRIMER_LEFT_1_SEQUENCE=AGGTGTTGCTGAGAGAGGTACTG
PRIMER_RIGHT_1_SEQUENCE=ACCAGGAGGTAGTTCTCATAGGC
PRIMER_LEFT_1=603,23
PRIMER_RIGHT_1=804,23
PRIMER_LEFT_1_TM=59.989
PRIMER_RIGHT_1_TM=60.035
PRIMER_LEFT_1_GC_PERCENT=52.174
PRIMER_RIGHT_1_GC_PERCENT=52.174
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_MISPRIMING_SCORE=12.00, MLT2C2 Interspersed repeat MLT2C2 - a consensus
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_1_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_1_END_STABILITY=5.7000
PRIMER_RIGHT_1_END_STABILITY=8.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=202
PRIMER_PAIR_PENALTY_2=0.0527
PRIMER_LEFT_2_PENALTY=0.009608
PRIMER_RIGHT_2_PENALTY=0.043085
PRIMER_LEFT_2_SEQUENCE=TTTGAGGATGATTGGTGAGAACT
PRIMER_RIGHT_2_SEQUENCE=AGTGTGTTGGTTTCGAGTCAAAT
PRIMER_LEFT_2=973,23
PRIMER_RIGHT_2=1936,23
PRIMER_LEFT_2_TM=59.990
PRIMER_RIGHT_2_TM=59.957
PRIMER_LEFT_2_GC_PERCENT=39.130
PRIMER_RIGHT_2_GC_PERCENT=39.130
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_2_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_2_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=964
PRIMER_PAIR_PENALTY_3=0.0532
PRIMER_LEFT_3_PENALTY=0.038458
PRIMER_RIGHT_3_PENALTY=0.014712
PRIMER_LEFT_3_SEQUENCE=TGAGAGAAAGACTGGAAGACCTG
PRIMER_RIGHT_3_SEQUENCE=GATCAATGACATGTAGTGCCTGA
PRIMER_LEFT_3=4076,23
PRIMER_RIGHT_3=4901,23
PRIMER_LEFT_3_TM=60.038
PRIMER_RIGHT_3_TM=60.015
PRIMER_LEFT_3_GC_PERCENT=47.826
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_3_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=826
PRIMER_PAIR_PENALTY_4=0.0573
PRIMER_LEFT_4_PENALTY=0.014220
PRIMER_RIGHT_4_PENALTY=0.043085
PRIMER_LEFT_4_SEQUENCE=GATGTCTGAACAATTGGCTTAGG
PRIMER_RIGHT_4_SEQUENCE=AGTGTGTTGGTTTCGAGTCAAAT
PRIMER_LEFT_4=1233,23
PRIMER_RIGHT_4=1936,23
PRIMER_LEFT_4_TM=60.014
PRIMER_RIGHT_4_TM=59.957
PRIMER_LEFT_4_GC_PERCENT=43.478
PRIMER_RIGHT_4_GC_PERCENT=39.130
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, MER34 a consensus
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_4_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_4_END_STABILITY=7.5000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=704
PRIMER_PAIR_PENALTY_5=0.0601
PRIMER_LEFT_5_PENALTY=0.038458
PRIMER_RIGHT_5_PENALTY=0.021648
PRIMER_LEFT_5_SEQUENCE=TGAGAGAAAGACTGGAAGACCTG
PRIMER_RIGHT_5_SEQUENCE=TTCAAGTGTTTCAAGGAGGGTAA
PRIMER_LEFT_5=4076,23
PRIMER_RIGHT_5=4611,23
PRIMER_LEFT_5_TM=60.038
PRIMER_RIGHT_5_TM=60.022
PRIMER_LEFT_5_GC_PERCENT=47.826
PRIMER_RIGHT_5_GC_PERCENT=39.130
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_5_MISPRIMING_SCORE=11.00, PTR5 Human PTR5 mRNA for repetitive sequence
PRIMER_PAIR_5_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_5_END_STABILITY=7.9000
PRIMER_RIGHT_5_END_STABILITY=7.2000
PRIMER_PAIR_5_COMPL_ANY=8.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_5=536
PRIMER_PAIR_PENALTY_6=0.0607
PRIMER_LEFT_6_PENALTY=0.017590
PRIMER_RIGHT_6_PENALTY=0.043085
PRIMER_LEFT_6_SEQUENCE=AAATCTAACAGACTTTCCTGCCC
PRIMER_RIGHT_6_SEQUENCE=AGTGTGTTGGTTTCGAGTCAAAT
PRIMER_LEFT_6=1513,23
PRIMER_RIGHT_6=1936,23
PRIMER_LEFT_6_TM=60.018
PRIMER_RIGHT_6_TM=59.957
PRIMER_LEFT_6_GC_PERCENT=43.478
PRIMER_RIGHT_6_GC_PERCENT=39.130
PRIMER_LEFT_6_SELF_ANY=5.00
PRIMER_RIGHT_6_SELF_ANY=5.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=3.00
PRIMER_LEFT_6_MISPRIMING_SCORE=11.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_6_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_6_MISPRIMING_SCORE=21.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_6_END_STABILITY=11.2000
PRIMER_RIGHT_6_END_STABILITY=7.2000
PRIMER_PAIR_6_COMPL_ANY=7.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_6=424
PRIMER_PAIR_PENALTY_7=0.0736
PRIMER_LEFT_7_PENALTY=0.051990
PRIMER_RIGHT_7_PENALTY=0.021648
PRIMER_LEFT_7_SEQUENCE=ATTTTATTTCAAGGGATAGGGCA
PRIMER_RIGHT_7_SEQUENCE=TTCAAGTGTTTCAAGGAGGGTAA
PRIMER_LEFT_7=3664,23
PRIMER_RIGHT_7=4611,23
PRIMER_LEFT_7_TM=60.052
PRIMER_RIGHT_7_TM=60.022
PRIMER_LEFT_7_GC_PERCENT=34.783
PRIMER_RIGHT_7_GC_PERCENT=39.130
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_MISPRIMING_SCORE=12.00, MER2 Nonautonomous DNA transposon
PRIMER_RIGHT_7_MISPRIMING_SCORE=11.00, PTR5 Human PTR5 mRNA for repetitive sequence
PRIMER_PAIR_7_MISPRIMING_SCORE=22.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_7_END_STABILITY=11.2000
PRIMER_RIGHT_7_END_STABILITY=7.2000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_7=948
PRIMER_PAIR_PENALTY_8=0.0790
PRIMER_LEFT_8_PENALTY=0.064245
PRIMER_RIGHT_8_PENALTY=0.014712
PRIMER_LEFT_8_SEQUENCE=TCTCCAGGTGTGCTTTCTTAGTC
PRIMER_RIGHT_8_SEQUENCE=GATCAATGACATGTAGTGCCTGA
PRIMER_LEFT_8=4644,23
PRIMER_RIGHT_8=4901,23
PRIMER_LEFT_8_TM=59.936
PRIMER_RIGHT_8_TM=60.015
PRIMER_LEFT_8_GC_PERCENT=47.826
PRIMER_RIGHT_8_GC_PERCENT=43.478
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_MISPRIMING_SCORE=12.00, reverse Tigger2 Autonomous DNA transposon
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_8_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_8_END_STABILITY=5.4000
PRIMER_RIGHT_8_END_STABILITY=8.2000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_8=258
PRIMER_PAIR_PENALTY_9=0.0818
PRIMER_LEFT_9_PENALTY=0.038744
PRIMER_RIGHT_9_PENALTY=0.043085
PRIMER_LEFT_9_SEQUENCE=ACAAACAATGGCTTTGTGACTTT
PRIMER_RIGHT_9_SEQUENCE=AGTGTGTTGGTTTCGAGTCAAAT
PRIMER_LEFT_9=1347,23
PRIMER_RIGHT_9=1936,23
PRIMER_LEFT_9_TM=59.961
PRIMER_RIGHT_9_TM=59.957
PRIMER_LEFT_9_GC_PERCENT=34.783
PRIMER_RIGHT_9_GC_PERCENT=39.130
PRIMER_LEFT_9_SELF_ANY=8.00
PRIMER_RIGHT_9_SELF_ANY=5.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=3.00
PRIMER_LEFT_9_MISPRIMING_SCORE=12.00, MER4C a consensus
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_9_MISPRIMING_SCORE=20.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_9_END_STABILITY=6.7000
PRIMER_RIGHT_9_END_STABILITY=7.2000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_9=590
PRIMER_PAIR_PENALTY_10=0.0901
PRIMER_LEFT_10_PENALTY=0.043085
PRIMER_RIGHT_10_PENALTY=0.046971
PRIMER_LEFT_10_SEQUENCE=ATTTGACTCGAAACCAACACACT
PRIMER_RIGHT_10_SEQUENCE=ACACCTCCACTGTTATGTATGGG
PRIMER_LEFT_10=1914,23
PRIMER_RIGHT_10=2536,23
PRIMER_LEFT_10_TM=59.957
PRIMER_RIGHT_10_TM=60.047
PRIMER_LEFT_10_GC_PERCENT=39.130
PRIMER_RIGHT_10_GC_PERCENT=47.826
PRIMER_LEFT_10_SELF_ANY=5.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_10_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_10_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_10_END_STABILITY=6.1000
PRIMER_RIGHT_10_END_STABILITY=9.6000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_10=623
PRIMER_PAIR_PENALTY_11=0.0914
PRIMER_LEFT_11_PENALTY=0.039409
PRIMER_RIGHT_11_PENALTY=0.051990
PRIMER_LEFT_11_SEQUENCE=CTAATTATTGGGGGTTTTCCTTG
PRIMER_RIGHT_11_SEQUENCE=TGCCCTATCCCTTGAAATAAAAT
PRIMER_LEFT_11=2727,23
PRIMER_RIGHT_11=3686,23
PRIMER_LEFT_11_TM=59.961
PRIMER_RIGHT_11_TM=60.052
PRIMER_LEFT_11_GC_PERCENT=39.130
PRIMER_RIGHT_11_GC_PERCENT=34.783
PRIMER_LEFT_11_SELF_ANY=6.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=2.00
PRIMER_LEFT_11_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, MER2 Nonautonomous DNA transposon
PRIMER_PAIR_11_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_11_END_STABILITY=8.5000
PRIMER_RIGHT_11_END_STABILITY=7.2000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_11=960
PRIMER_PAIR_PENALTY_12=0.0915
PRIMER_LEFT_12_PENALTY=0.069862
PRIMER_RIGHT_12_PENALTY=0.021648
PRIMER_LEFT_12_SEQUENCE=GGGATAGGGCAGATTACCATTAG
PRIMER_RIGHT_12_SEQUENCE=TTCAAGTGTTTCAAGGAGGGTAA
PRIMER_LEFT_12=3676,23
PRIMER_RIGHT_12=4611,23
PRIMER_LEFT_12_TM=60.070
PRIMER_RIGHT_12_TM=60.022
PRIMER_LEFT_12_GC_PERCENT=47.826
PRIMER_RIGHT_12_GC_PERCENT=39.130
PRIMER_LEFT_12_SELF_ANY=2.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_MISPRIMING_SCORE=11.00, L1PA7 3'-end of L1 repeat (subfamily L1PA7) - a consensus sequence
PRIMER_RIGHT_12_MISPRIMING_SCORE=11.00, PTR5 Human PTR5 mRNA for repetitive sequence
PRIMER_PAIR_12_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_12_END_STABILITY=5.9000
PRIMER_RIGHT_12_END_STABILITY=7.2000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_12=936
PRIMER_PAIR_PENALTY_13=0.0915
PRIMER_LEFT_13_PENALTY=0.069862
PRIMER_RIGHT_13_PENALTY=0.021648
PRIMER_LEFT_13_SEQUENCE=AGGGATAGGGCAGATTACCATTA
PRIMER_RIGHT_13_SEQUENCE=TTCAAGTGTTTCAAGGAGGGTAA
PRIMER_LEFT_13=3675,23
PRIMER_RIGHT_13=4611,23
PRIMER_LEFT_13_TM=60.070
PRIMER_RIGHT_13_TM=60.022
PRIMER_LEFT_13_GC_PERCENT=43.478
PRIMER_RIGHT_13_GC_PERCENT=39.130
PRIMER_LEFT_13_SELF_ANY=2.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=2.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_MISPRIMING_SCORE=12.00, MER7B Nonautonomous DNA transposon
PRIMER_RIGHT_13_MISPRIMING_SCORE=11.00, PTR5 Human PTR5 mRNA for repetitive sequence
PRIMER_PAIR_13_MISPRIMING_SCORE=21.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_13_END_STABILITY=6.2000
PRIMER_RIGHT_13_END_STABILITY=7.2000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_13=937
PRIMER_PAIR_PENALTY_14=0.0921
PRIMER_LEFT_14_PENALTY=0.009608
PRIMER_RIGHT_14_PENALTY=0.082508
PRIMER_LEFT_14_SEQUENCE=TTTGAGGATGATTGGTGAGAACT
PRIMER_RIGHT_14_SEQUENCE=GTACAAATATGGTCAAAGGCAGC
PRIMER_LEFT_14=973,23
PRIMER_RIGHT_14=1467,23
PRIMER_LEFT_14_TM=59.990
PRIMER_RIGHT_14_TM=59.917
PRIMER_LEFT_14_GC_PERCENT=39.130
PRIMER_RIGHT_14_GC_PERCENT=43.478
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=2.00
PRIMER_RIGHT_14_SELF_END=3.00
PRIMER_LEFT_14_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_14_MISPRIMING_SCORE=12.00, reverse L1MB7 3'-end of L1 repeat (subfamily L1MB7) - a consensus sequence
PRIMER_PAIR_14_MISPRIMING_SCORE=23.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_14_END_STABILITY=6.4000
PRIMER_RIGHT_14_END_STABILITY=9.7000
PRIMER_PAIR_14_COMPL_ANY=7.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_14=495
PRIMER_PAIR_PENALTY_15=0.0926
PRIMER_LEFT_15_PENALTY=0.043085
PRIMER_RIGHT_15_PENALTY=0.049500
PRIMER_LEFT_15_SEQUENCE=ATTTGACTCGAAACCAACACACT
PRIMER_RIGHT_15_SEQUENCE=TGGTTAGACAAAACACCTCCACT
PRIMER_LEFT_15=1914,23
PRIMER_RIGHT_15=2548,23
PRIMER_LEFT_15_TM=59.957
PRIMER_RIGHT_15_TM=59.950
PRIMER_LEFT_15_GC_PERCENT=39.130
PRIMER_RIGHT_15_GC_PERCENT=43.478
PRIMER_LEFT_15_SELF_ANY=5.00
PRIMER_RIGHT_15_SELF_ANY=5.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_15_MISPRIMING_SCORE=12.00, reverse MER21B a consensus
PRIMER_PAIR_15_MISPRIMING_SCORE=21.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_15_END_STABILITY=6.1000
PRIMER_RIGHT_15_END_STABILITY=7.9000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_15=635
PRIMER_PAIR_PENALTY_16=0.0926
PRIMER_LEFT_16_PENALTY=0.043085
PRIMER_RIGHT_16_PENALTY=0.049500
PRIMER_LEFT_16_SEQUENCE=ATTTGACTCGAAACCAACACACT
PRIMER_RIGHT_16_SEQUENCE=GGTTAGACAAAACACCTCCACTG
PRIMER_LEFT_16=1914,23
PRIMER_RIGHT_16=2547,23
PRIMER_LEFT_16_TM=59.957
PRIMER_RIGHT_16_TM=59.950
PRIMER_LEFT_16_GC_PERCENT=39.130
PRIMER_RIGHT_16_GC_PERCENT=47.826
PRIMER_LEFT_16_SELF_ANY=5.00
PRIMER_RIGHT_16_SELF_ANY=3.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=3.00
PRIMER_LEFT_16_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_16_MISPRIMING_SCORE=11.00, MLT2C2 Interspersed repeat MLT2C2 - a consensus
PRIMER_PAIR_16_MISPRIMING_SCORE=20.00, MLT2C2 Interspersed repeat MLT2C2 - a consensus
PRIMER_LEFT_16_END_STABILITY=6.1000
PRIMER_RIGHT_16_END_STABILITY=6.7000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_16=634
PRIMER_PAIR_PENALTY_17=0.0935
PRIMER_LEFT_17_PENALTY=0.010990
PRIMER_RIGHT_17_PENALTY=0.082508
PRIMER_LEFT_17_SEQUENCE=AGGTGTTGCTGAGAGAGGTACTG
PRIMER_RIGHT_17_SEQUENCE=GTACAAATATGGTCAAAGGCAGC
PRIMER_LEFT_17=603,23
PRIMER_RIGHT_17=1467,23
PRIMER_LEFT_17_TM=59.989
PRIMER_RIGHT_17_TM=59.917
PRIMER_LEFT_17_GC_PERCENT=52.174
PRIMER_RIGHT_17_GC_PERCENT=43.478
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=3.00
PRIMER_LEFT_17_MISPRIMING_SCORE=12.00, MLT2C2 Interspersed repeat MLT2C2 - a consensus
PRIMER_RIGHT_17_MISPRIMING_SCORE=12.00, reverse L1MB7 3'-end of L1 repeat (subfamily L1MB7) - a consensus sequence
PRIMER_PAIR_17_MISPRIMING_SCORE=22.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_17_END_STABILITY=5.7000
PRIMER_RIGHT_17_END_STABILITY=9.7000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_17=865
PRIMER_PAIR_PENALTY_18=0.0967
PRIMER_LEFT_18_PENALTY=0.014220
PRIMER_RIGHT_18_PENALTY=0.082508
PRIMER_LEFT_18_SEQUENCE=GATGTCTGAACAATTGGCTTAGG
PRIMER_RIGHT_18_SEQUENCE=GTACAAATATGGTCAAAGGCAGC
PRIMER_LEFT_18=1233,23
PRIMER_RIGHT_18=1467,23
PRIMER_LEFT_18_TM=60.014
PRIMER_RIGHT_18_TM=59.917
PRIMER_LEFT_18_GC_PERCENT=43.478
PRIMER_RIGHT_18_GC_PERCENT=43.478
PRIMER_LEFT_18_SELF_ANY=6.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=2.00
PRIMER_RIGHT_18_SELF_END=3.00
PRIMER_LEFT_18_MISPRIMING_SCORE=12.00, MER34 a consensus
PRIMER_RIGHT_18_MISPRIMING_SCORE=12.00, reverse L1MB7 3'-end of L1 repeat (subfamily L1MB7) - a consensus sequence
PRIMER_PAIR_18_MISPRIMING_SCORE=23.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_18_END_STABILITY=7.5000
PRIMER_RIGHT_18_END_STABILITY=9.7000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_18=235
PRIMER_PAIR_PENALTY_19=0.1031
PRIMER_LEFT_19_PENALTY=0.010990
PRIMER_RIGHT_19_PENALTY=0.092080
PRIMER_LEFT_19_SEQUENCE=AGGTGTTGCTGAGAGAGGTACTG
PRIMER_RIGHT_19_SEQUENCE=CGGATAAAGACCAGGAGGTAGTT
PRIMER_LEFT_19=603,23
PRIMER_RIGHT_19=813,23
PRIMER_LEFT_19_TM=59.989
PRIMER_RIGHT_19_TM=59.908
PRIMER_LEFT_19_GC_PERCENT=52.174
PRIMER_RIGHT_19_GC_PERCENT=47.826
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=2.00
PRIMER_LEFT_19_MISPRIMING_SCORE=12.00, MLT2C2 Interspersed repeat MLT2C2 - a consensus
PRIMER_RIGHT_19_MISPRIMING_SCORE=12.00, LTR9 LTR from human endogenous retrovirus-like sequence (HUERS-P3)
PRIMER_PAIR_19_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_19_END_STABILITY=5.7000
PRIMER_RIGHT_19_END_STABILITY=5.7000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_19=211
=