PRIMER_SEQUENCE_ID=NM_015466
SEQUENCE=ACGCGTCCGGCAGCTGCAGCAGCTACGGGAGTGGCCGGGTGGCCGGCGGGTGCCAGCCGCCATGGAGGCCGTGCCCCGCATGCCCATGATCTGGCTGGACCTGAAGGAGGCCGGTGACTTTCACTTCCAGCCAGCTGTGAAGAAGTTTGTCCTGAAGAATTATGGAGAGAACCCAGAAGCCTACAATGAAGAACTGAAGAAGCTGGAGTTGCTCAGACAGAATGCTGTCCGTGTCCCACGAGACTTTGAGGGCTGTAGTGTCCTCCGCAAGTACCTCGGCCAGCTTCATTACCTGCAGAGTCGGGTCCCCATGGGCTCGGGCCAGGAGGCCGCTGTCCCTGTCACCTGGACAGAGATCTTCTCAGGCAAGTCTGTGGCCCATGAGGACATCAAGTACGAGCAGGCCTGTATTCTCTACAACCTTGGAGCGCTGCACTCCATGCTGGGGGCCATGGACAAGCGGGTGTCTGAGGAGGGCATGAAGGTCTCCTGTACCCATTTCCAGTGCGCAGCCGGCGCCTTCGCCTACCTACGGGAGCACTTCCCTCAAGCCTACAGCGTCGACATGAGCCGCCAGATCCTTACGCTCAACGTCAACCTCATGCTGGGCCAGGCTCAGGAGTGCCTCCTGGAGAAGTCGATGTTGGACAACAGGAAGAGCTTTCTGGTGGCCCGCATCAGTGCACAGGTGGTAGATTACTACAAGGAGGCATGCCGGGCCTTGGAGAACCCCGACACTGCCTCACTGCTGGGCCGGATCCAGAAGGACTGGAAGAAACTTGTGCAGATGAAGATCTACTACTTCGCAGCCGTGGCTCATCTGCACATGGGAAAGCAGGCCGAGGAGCAGCAGAAGTTCGGGGAGCGGGTTGCATACTTCCAGAGCGCCCTGGACAAGCTCAATGAAGCCATCAAGTTGGCCAAGGGCCAGCCTGACACTGTGCAAGACGCGCTTCGCTTCACTATGGATGTCATTGGGGGAAAGTACAATTCTGCCAAGAAGGACAACGACTTCATTTACCATGAGGCTGTCCCAGCATTGGACACTCTTCAGCCTGTAAAAGGAGCCCCCTTGGTGAAGCCCTTGCCAGTGAACCCCACAGACCCAGCTGTTACAGGCCCTGACATCTTTGCCAAACTGGTACCCATGGCTGCCCACGAGGCCTCGTCACTGTACAGTGAGGAGAAGGCCAAGCTGCTCCGGGAGATGATGGCCAAGATTGAGGACAAGAATGAGGTCCTGGACCAGTTCATGGATTCAATGCAGTTGGATCCCGAGACGGTGGACAACCTTGATGCCTACAGCCACATCCCACCCCAGCTCATGGAGAAGTGCGCGGCTCTCAGCGTCCGGCCCGACACTGTCAGGAACCTTGTACAGTCCATGCAAGTGCTGTCAGGTGTGTTCACGGATGTGGAGGCTTCCCTGAAGGACATCAGAGATCTGTTGGAGGAGGATGAGCTGCTAGAGCAGAAGTTTCAGGAGGCGGTGGGCCAGGCAGGGGCCATCTCCATCACCTCCAAGGCTGAGCTGGCAGAGGTGAGGCGAGAATGGGCCAAGTACATGGAAGTCCATGAGAAGGCCTCCTTCACCAACAGTGAGCTGCACCGTGCCATGAACCTGCACGTCGGCAACCTGCGCCTGCTCAGCGGGCCGCTTGACCAGGTCCGGGCTGCCCTGCCCACACCGGCCCTCTCCCCAGAGGACAAGGCCGTGCTGCAAAACCTAAAGCGCATCCTGGCTAAGGTGCAGGAGATGCGGGACCAGCGCGTGTCCCTGGAGCAGCAGCTGCGTGAGCTTATCCAGAAAGATGACATCACTGCCTCGCTGGTCACCACAGACCACTCAGAGATGAAGAAGTTGTTCGAGGAGCAGCTGAAAAAGTATGACCAGCTGAAGGTGTACCTGGAGCAGAACCTGGCCGCCCAGGACCGTGTCCTCTGTGCACTGACAGAGGCCAACGTGCAGTACGCAGCCGTGCGGCGGGTACTCAGCGACTTGGACCAAAAGTGGAACTCCACGCTGCAGACCCTGGTGGCCTCGTATGAAGCCTATGAGGACCTGATGAAGAAGTCGCAGGAGGGCAGGGACTTCTACGCAGATCTGGAGAGCAAGGTGGCTGCTCTGCTGGAGCGCACGCAGTCCACCTGCCAGGCCCGCGAGGCTGCCCGCCAGCAGCTCCTGGACAGGGAGCTGAAGAAGAAGCCGCCGCCACGGCCCACAGCCCCAAAGCCGCTGCTGCCCCGCAGGGAGGAGAGTGAGGCAGTGGAAGCAGGAGACCCCCCTGAGGAGCTGCGCAGCCTCCCCCCTGACATGGTGGCTGGCCCACGACTGCCTGACACCTTCCTGGGAAGTGCCACCCCGCTCCACTTTCCTCCCAGCCCCTTCCCCAGCTCCACAGGCCCAGGACCCCACTATCTCTCAGGCCCCTTGCCCCCTGGTACCTACTCGGGCCCCACCCAGCTGATACAGCCCAGGGCCCCAGGGCCCCATGCAATGCCCGTAGCACCTGGGCCTGCCCTCTACCCAGCCCCTGCCTACACACCGGAGCTGGGCCTTGTGCCCCGATCCTCCCCACAGCATGGCGTGGTGAGCAGTCCCTATGTGGGGGTAGGGCCGGCCCCACCAGTTGCAGGTCTCCCCTCGGCCCCACCTCCTCAATTCTCAGGCCCCGAGTTGGCCATGGCGGTTCGGCCAGCCACCACCACAGTAGATAGCATCCAGGCGCCCATCCCCAGCCACACAGCCCCACGGCCAAACCCCACCCCTGCTCCTCCCCCGCCCTGCTTCCCTGTGCCCCCACCGCAGCCACTGCCCACGCCTTACACCTACCCTGCAGGGGCTAAGCAACCCATCCCGGCACAGCACCACTTCTCTTCTGGGATCCCCGCAGGTTTTCCAGCCCCAAGGATTGGGCCCCAGCCCCAGCCCCATCCTCAGCCCCATCCTTCACAAGCGTTTGGGCCTCAGCCCCCACAGCAGCCCCTTCCACTCCAGCATCCACATCTCTTCCCACCCCAGGCCCCAGGACTCCTACCCCCACAATCCCCCTACCCCTATGCCCCTCAGCCTGGGGTCCTGGGGCAGCCGCCACCCCCCCTACACACCCAGCTCTACCCAGGTCCCGCTCAAGACCCTCTGCCAGCCCACTCAGGGGCTCTGCCTTTCCCCAGCCCTGGGCCCCCTCAGCCTCCCCATCCCCCACTGGCATATGGTCCTGCCCCTTCTACCAGACCCATGGGCCCCCAGGCAGCCCCTCTTACCATTCGAGGGCCCTCGTCTGCTGGCCAGTCCACCCCTAGTCCCCACCTGGTGCCTTCACCTGCCCCATCTCCAGGGCCTGGTCCGGTACCCCCTCGCCCCCCAGCAGCAGAACCACCCCCTTGCCTGCGCCGAGGCGCCGCAGCTGCAGACCTGCTCTCCTCCAGCCCGGAGAGCCAGCATGGCGGCACTCAGTCTCCTGGGGGTGGGCAGCCCCTGCTGCAGCCCACCAAGGTGGATGCAGCTGAGGGTCGTCGGCCGCAGGCCCTGCGGCTGATTGAGCGGGACCCCTATGAGCATCCTGAGAGGCTGCGGCAGTTGCAGCAGGAGCTGGAGGCCTTTCGGGGTCAGCTGGGGGATGTGGGAGCTCTGGACACTGTCTGGCGAGAGCTGCAAGATGCGCAGGAACATGATGCCCGAGGCCGTTCCATCGCCATTGCCCGCTGCTACTCACTGAAGAACCGGCACCAGGATGTCATGCCCTATGACAGTAACCGTGTGGTGCTGCGCTCAGGCAAGGATGACTACATCAATGCCAGCTGCGTGGAGGGGCTCTCCCCATACTGCCCCCCGCTAGTGGCAACCCAGGCCCCACTGCCTGGCACAGCTGCTGACTTCTGGCTCATGGTCCATGAGCAGAAAGTGTCAGTCATTGTCATGCTGGTTTCTGAGGCTGAGATGGAGAAGCAAAAAGTGGCACGCTACTTCCCCACCGAGAGGGGCCAGCCCATGGTGCACGGTGCCCTGAGCCTGGCATTGAGCAGCGTCCGCAGCACCGAAACCCATGTGGAGCGCGTGCTGAGCCTGCAGTTCCGAGACCAGAGCCTCAAGCGCTCTCTTGTGCACCTGCACTTCCCCACTTGGCCTGAGTTAGGCCTGCCCGACAGCCCCAGCAACTTGCTGCGCTTCATCCAGGAGGTGCACGCACATTACCTGCATCAGCGGCCGCTGCACACGCCCATCATTGTGCACTGCAGCTCTGGTGTGGGCCGCACGGGAGCCTTTGCACTGCTCTATGCAGCTGTGCAGGAGGTGGAGGCTGGGAACGGAATCCCTGAGCTGCCTCAGCTGGTGCGGCGCATGCGGCAGCAGAGAAAGCACATGCTGCAGGAGAAGCTGCACCTCAGGTTCTGCTATGAGGCAGTGGTGAGACACGTGGAGCAGGTCCTGCAGCGCCATGGTGTGCCTCCTCCATGCAAACCCTTGGCCAGTGCAAGCATCAGCCAGAAGAACCACCTTCCTCAGGACTCCCAGGACCTGGTCCTCGGTGGGGATGTGCCCATCAGCTCCATCCAGGCCACCATTGCCAAGCTCAGCATTCGGCCTCCTGGGGGGTTGGAGTCCCCGGTTGCCAGCTTGCCAGGCCCTGCAGAGCCCCCAGGCCTCCCGCCAGCCAGCCTCCCAGAGTCTACCCCAATCCCATCTTCCTCCCCACCCCCCCTTTCCTCCCCACTACCTGAGGCTCCCCAGCCTAAGGAGGAGCCGCCAGTGCCTGAAGCCCCCAGCTCGGGGCCCCCCTCCTCCTCCCTGGAATTGCTGGCCTCCTTGACCCCAGAGGCCTTCTCCCTGGACAGCTCCCTGCGGGGCAAACAGCGGATGAGCAAGCATAACTTTCTGCAGGCCCATAACGGGCAAGGGCTGCGGGCCACCCGGCCCTCTGACGACCCCCTCAGCCTTCTGGATCCACTCTGGACACTCAACAAGACCTGAACAGGTTTTGCCTACCTGGTCCTTACACTACATCATCATCATCTCATGCCCACCTGCCCACACCCAGCAGAGCTTCTCAGTGGGCACAGTCTCTTACTCCCATTTCTGCTGCCTTTGGCCCTGCCTGGCCCAGCCTGCACCCCTGTGGGGTGGAAATGTACTGCAGGCTCTGGGTCAGGTTCTGCTCCTTTATGGGACCCGACATTTTTCAGCTCTTTGCTATTGAAATAATAAACCACCCTGTTCTGTGGCCCGTAAAAAAAAAAAAAAAAAAAA
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 60573, GC content failed 1984, low tm 11924, high tm 35852, high any compl 10, high end compl 54, high repeat similarity 47, long poly-x seq 166, ok 10536
PRIMER_RIGHT_EXPLAIN=considered 60499, GC content failed 1838, low tm 12269, high tm 35457, high any compl 2, high end compl 13, high repeat similarity 9, long poly-x seq 178, ok 10733
PRIMER_PAIR_EXPLAIN=considered 2554, unacceptable product size 2483, high end compl 6, ok 65
PRIMER_PAIR_PENALTY=0.0956
PRIMER_LEFT_PENALTY=0.073619
PRIMER_RIGHT_PENALTY=0.022025
PRIMER_LEFT_SEQUENCE=GAGCAGGCCTGTATTCTCTACAA
PRIMER_RIGHT_SEQUENCE=ACTTGCATGGACTGTACAAGGTT
PRIMER_LEFT=397,23
PRIMER_RIGHT=1391,23
PRIMER_LEFT_TM=59.926
PRIMER_RIGHT_TM=59.978
PRIMER_LEFT_GC_PERCENT=47.826
PRIMER_RIGHT_GC_PERCENT=43.478
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_END_STABILITY=6.0000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=995
PRIMER_PAIR_PENALTY_1=0.1274
PRIMER_LEFT_1_PENALTY=0.047652
PRIMER_RIGHT_1_PENALTY=0.079770
PRIMER_LEFT_1_SEQUENCE=TGAAGAATTATGGAGAGAACCCA
PRIMER_RIGHT_1_SEQUENCE=TGGTAAATGAAGTCGTTGTCCTT
PRIMER_LEFT_1=152,23
PRIMER_RIGHT_1=1022,23
PRIMER_LEFT_1_TM=59.952
PRIMER_RIGHT_1_TM=59.920
PRIMER_LEFT_1_GC_PERCENT=39.130
PRIMER_RIGHT_1_GC_PERCENT=39.130
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_1_MISPRIMING_SCORE=21.00, L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=871
PRIMER_PAIR_PENALTY_2=0.1534
PRIMER_LEFT_2_PENALTY=0.073619
PRIMER_RIGHT_2_PENALTY=0.079770
PRIMER_LEFT_2_SEQUENCE=GAGCAGGCCTGTATTCTCTACAA
PRIMER_RIGHT_2_SEQUENCE=TGGTAAATGAAGTCGTTGTCCTT
PRIMER_LEFT_2=397,23
PRIMER_RIGHT_2=1022,23
PRIMER_LEFT_2_TM=59.926
PRIMER_RIGHT_2_TM=59.920
PRIMER_LEFT_2_GC_PERCENT=47.826
PRIMER_RIGHT_2_GC_PERCENT=39.130
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_2_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_2_MISPRIMING_SCORE=23.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_2_END_STABILITY=6.0000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=626
PRIMER_PAIR_PENALTY_3=0.1598
PRIMER_LEFT_3_PENALTY=0.010990
PRIMER_RIGHT_3_PENALTY=0.148815
PRIMER_LEFT_3_SEQUENCE=CTCAGTGGGCACAGTCTCTTACT
PRIMER_RIGHT_3_SEQUENCE=CCACAGAACAGGGTGGTTTATTA
PRIMER_LEFT_3=5047,23
PRIMER_RIGHT_3=5222,23
PRIMER_LEFT_3_TM=59.989
PRIMER_RIGHT_3_TM=60.149
PRIMER_LEFT_3_GC_PERCENT=52.174
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, reverse Tigger2 Autonomous DNA transposon
PRIMER_RIGHT_3_MISPRIMING_SCORE=11.00, MLT1b (MLT1b subfamily) - consensus sequence
PRIMER_PAIR_3_MISPRIMING_SCORE=20.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_3_END_STABILITY=5.7000
PRIMER_RIGHT_3_END_STABILITY=5.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=176
PRIMER_PAIR_PENALTY_4=0.1697
PRIMER_LEFT_4_PENALTY=0.047652
PRIMER_RIGHT_4_PENALTY=0.122060
PRIMER_LEFT_4_SEQUENCE=TGAAGAATTATGGAGAGAACCCA
PRIMER_RIGHT_4_SEQUENCE=AAATGGGTACAGGAGACCTTCAT
PRIMER_LEFT_4=152,23
PRIMER_RIGHT_4=500,23
PRIMER_LEFT_4_TM=59.952
PRIMER_RIGHT_4_TM=60.122
PRIMER_LEFT_4_GC_PERCENT=39.130
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_4_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=349
PRIMER_PAIR_PENALTY_5=0.1957
PRIMER_LEFT_5_PENALTY=0.073619
PRIMER_RIGHT_5_PENALTY=0.122060
PRIMER_LEFT_5_SEQUENCE=GAGCAGGCCTGTATTCTCTACAA
PRIMER_RIGHT_5_SEQUENCE=AAATGGGTACAGGAGACCTTCAT
PRIMER_LEFT_5=397,23
PRIMER_RIGHT_5=500,23
PRIMER_LEFT_5_TM=59.926
PRIMER_RIGHT_5_TM=60.122
PRIMER_LEFT_5_GC_PERCENT=47.826
PRIMER_RIGHT_5_GC_PERCENT=43.478
PRIMER_LEFT_5_SELF_ANY=8.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=2.00
PRIMER_RIGHT_5_SELF_END=2.00
PRIMER_LEFT_5_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_5_MISPRIMING_SCORE=12.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_5_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_5_END_STABILITY=6.0000
PRIMER_RIGHT_5_END_STABILITY=6.9000
PRIMER_PAIR_5_COMPL_ANY=6.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_5=104
PRIMER_PAIR_PENALTY_6=0.2108
PRIMER_LEFT_6_PENALTY=0.062009
PRIMER_RIGHT_6_PENALTY=0.148815
PRIMER_LEFT_6_SEQUENCE=TTGCCTACCTGGTCCTTACACTA
PRIMER_RIGHT_6_SEQUENCE=CCACAGAACAGGGTGGTTTATTA
PRIMER_LEFT_6=4979,23
PRIMER_RIGHT_6=5222,23
PRIMER_LEFT_6_TM=60.062
PRIMER_RIGHT_6_TM=60.149
PRIMER_LEFT_6_GC_PERCENT=47.826
PRIMER_RIGHT_6_GC_PERCENT=43.478
PRIMER_LEFT_6_SELF_ANY=5.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=2.00
PRIMER_RIGHT_6_SELF_END=3.00
PRIMER_LEFT_6_MISPRIMING_SCORE=11.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_6_MISPRIMING_SCORE=11.00, MLT1b (MLT1b subfamily) - consensus sequence
PRIMER_PAIR_6_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_6_END_STABILITY=5.7000
PRIMER_RIGHT_6_END_STABILITY=5.2000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_6=244
PRIMER_PAIR_PENALTY_7=0.2217
PRIMER_LEFT_7_PENALTY=0.141958
PRIMER_RIGHT_7_PENALTY=0.079770
PRIMER_LEFT_7_SEQUENCE=GAGAGAACCCAGAAGCCTACAAT
PRIMER_RIGHT_7_SEQUENCE=TGGTAAATGAAGTCGTTGTCCTT
PRIMER_LEFT_7=164,23
PRIMER_RIGHT_7=1022,23
PRIMER_LEFT_7_TM=60.142
PRIMER_RIGHT_7_TM=59.920
PRIMER_LEFT_7_GC_PERCENT=47.826
PRIMER_RIGHT_7_GC_PERCENT=39.130
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=2.00
PRIMER_RIGHT_7_SELF_END=2.00
PRIMER_LEFT_7_MISPRIMING_SCORE=12.00, reverse MLT1f (MLT1f subfamily) - consensus sequence
PRIMER_RIGHT_7_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_7_MISPRIMING_SCORE=21.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_7_END_STABILITY=6.6000
PRIMER_RIGHT_7_END_STABILITY=8.2000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_7=859
PRIMER_PAIR_PENALTY_8=0.2261
PRIMER_LEFT_8_PENALTY=0.047652
PRIMER_RIGHT_8_PENALTY=0.178426
PRIMER_LEFT_8_SEQUENCE=TGAAGAATTATGGAGAGAACCCA
PRIMER_RIGHT_8_SEQUENCE=TTACAGGCTGAAGAGTGTCCAAT
PRIMER_LEFT_8=152,23
PRIMER_RIGHT_8=1060,23
PRIMER_LEFT_8_TM=59.952
PRIMER_RIGHT_8_TM=60.178
PRIMER_LEFT_8_GC_PERCENT=39.130
PRIMER_RIGHT_8_GC_PERCENT=43.478
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=5.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=2.00
PRIMER_LEFT_8_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_8_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_8_END_STABILITY=9.4000
PRIMER_RIGHT_8_END_STABILITY=8.4000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_8=909
PRIMER_PAIR_PENALTY_9=0.2520
PRIMER_LEFT_9_PENALTY=0.073619
PRIMER_RIGHT_9_PENALTY=0.178426
PRIMER_LEFT_9_SEQUENCE=GAGCAGGCCTGTATTCTCTACAA
PRIMER_RIGHT_9_SEQUENCE=TTACAGGCTGAAGAGTGTCCAAT
PRIMER_LEFT_9=397,23
PRIMER_RIGHT_9=1060,23
PRIMER_LEFT_9_TM=59.926
PRIMER_RIGHT_9_TM=60.178
PRIMER_LEFT_9_GC_PERCENT=47.826
PRIMER_RIGHT_9_GC_PERCENT=43.478
PRIMER_LEFT_9_SELF_ANY=8.00
PRIMER_RIGHT_9_SELF_ANY=5.00
PRIMER_LEFT_9_SELF_END=2.00
PRIMER_RIGHT_9_SELF_END=2.00
PRIMER_LEFT_9_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_9_MISPRIMING_SCORE=24.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_9_END_STABILITY=6.0000
PRIMER_RIGHT_9_END_STABILITY=8.4000
PRIMER_PAIR_9_COMPL_ANY=7.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_9=664
PRIMER_PAIR_PENALTY_10=0.2640
PRIMER_LEFT_10_PENALTY=0.141958
PRIMER_RIGHT_10_PENALTY=0.122060
PRIMER_LEFT_10_SEQUENCE=GAGAGAACCCAGAAGCCTACAAT
PRIMER_RIGHT_10_SEQUENCE=AAATGGGTACAGGAGACCTTCAT
PRIMER_LEFT_10=164,23
PRIMER_RIGHT_10=500,23
PRIMER_LEFT_10_TM=60.142
PRIMER_RIGHT_10_TM=60.122
PRIMER_LEFT_10_GC_PERCENT=47.826
PRIMER_RIGHT_10_GC_PERCENT=43.478
PRIMER_LEFT_10_SELF_ANY=3.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=2.00
PRIMER_RIGHT_10_SELF_END=2.00
PRIMER_LEFT_10_MISPRIMING_SCORE=12.00, reverse MLT1f (MLT1f subfamily) - consensus sequence
PRIMER_RIGHT_10_MISPRIMING_SCORE=12.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_10_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_10_END_STABILITY=6.6000
PRIMER_RIGHT_10_END_STABILITY=6.9000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_10=337
PRIMER_PAIR_PENALTY_11=0.3204
PRIMER_LEFT_11_PENALTY=0.141958
PRIMER_RIGHT_11_PENALTY=0.178426
PRIMER_LEFT_11_SEQUENCE=GAGAGAACCCAGAAGCCTACAAT
PRIMER_RIGHT_11_SEQUENCE=TTACAGGCTGAAGAGTGTCCAAT
PRIMER_LEFT_11=164,23
PRIMER_RIGHT_11=1060,23
PRIMER_LEFT_11_TM=60.142
PRIMER_RIGHT_11_TM=60.178
PRIMER_LEFT_11_GC_PERCENT=47.826
PRIMER_RIGHT_11_GC_PERCENT=43.478
PRIMER_LEFT_11_SELF_ANY=3.00
PRIMER_RIGHT_11_SELF_ANY=5.00
PRIMER_LEFT_11_SELF_END=2.00
PRIMER_RIGHT_11_SELF_END=2.00
PRIMER_LEFT_11_MISPRIMING_SCORE=12.00, reverse MLT1f (MLT1f subfamily) - consensus sequence
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_11_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_11_END_STABILITY=6.6000
PRIMER_RIGHT_11_END_STABILITY=8.4000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_11=897
PRIMER_PAIR_PENALTY_12=0.3398
PRIMER_LEFT_12_PENALTY=0.317733
PRIMER_RIGHT_12_PENALTY=0.022025
PRIMER_LEFT_12_SEQUENCE=CAAGAAGGACAACGACTTCATTT
PRIMER_RIGHT_12_SEQUENCE=ACTTGCATGGACTGTACAAGGTT
PRIMER_LEFT_12=996,23
PRIMER_RIGHT_12=1391,23
PRIMER_LEFT_12_TM=59.682
PRIMER_RIGHT_12_TM=59.978
PRIMER_LEFT_12_GC_PERCENT=39.130
PRIMER_RIGHT_12_GC_PERCENT=43.478
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=6.00
PRIMER_LEFT_12_SELF_END=2.00
PRIMER_RIGHT_12_SELF_END=2.00
PRIMER_LEFT_12_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_12_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_12_MISPRIMING_SCORE=22.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_12_END_STABILITY=7.2000
PRIMER_RIGHT_12_END_STABILITY=7.9000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_12=396
PRIMER_PAIR_PENALTY_13=0.3481
PRIMER_LEFT_13_PENALTY=0.022025
PRIMER_RIGHT_13_PENALTY=0.326043
PRIMER_LEFT_13_SEQUENCE=AACCTTGTACAGTCCATGCAAGT
PRIMER_RIGHT_13_SEQUENCE=CTCTCCAGATCTGCGTAGAAGTC
PRIMER_LEFT_13=1369,23
PRIMER_RIGHT_13=2111,23
PRIMER_LEFT_13_TM=59.978
PRIMER_RIGHT_13_TM=59.674
PRIMER_LEFT_13_GC_PERCENT=43.478
PRIMER_RIGHT_13_GC_PERCENT=52.174
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=8.00
PRIMER_LEFT_13_SELF_END=3.00
PRIMER_RIGHT_13_SELF_END=2.00
PRIMER_LEFT_13_MISPRIMING_SCORE=11.00, MER6 Nonautonomous DNA transposon
PRIMER_RIGHT_13_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_13_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_13_END_STABILITY=6.7000
PRIMER_RIGHT_13_END_STABILITY=6.4000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_13=743
PRIMER_PAIR_PENALTY_14=0.3654
PRIMER_LEFT_14_PENALTY=0.047652
PRIMER_RIGHT_14_PENALTY=0.317733
PRIMER_LEFT_14_SEQUENCE=TGAAGAATTATGGAGAGAACCCA
PRIMER_RIGHT_14_SEQUENCE=AAATGAAGTCGTTGTCCTTCTTG
PRIMER_LEFT_14=152,23
PRIMER_RIGHT_14=1018,23
PRIMER_LEFT_14_TM=59.952
PRIMER_RIGHT_14_TM=59.682
PRIMER_LEFT_14_GC_PERCENT=39.130
PRIMER_RIGHT_14_GC_PERCENT=39.130
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_14_MISPRIMING_SCORE=12.00, L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_PAIR_14_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_14_END_STABILITY=9.4000
PRIMER_RIGHT_14_END_STABILITY=7.0000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_14=867
PRIMER_PAIR_PENALTY_15=0.3715
PRIMER_LEFT_15_PENALTY=0.349469
PRIMER_RIGHT_15_PENALTY=0.022025
PRIMER_LEFT_15_SEQUENCE=ATCCAGAAGGACTGGAAGAAACT
PRIMER_RIGHT_15_SEQUENCE=ACTTGCATGGACTGTACAAGGTT
PRIMER_LEFT_15=757,23
PRIMER_RIGHT_15=1391,23
PRIMER_LEFT_15_TM=59.651
PRIMER_RIGHT_15_TM=59.978
PRIMER_LEFT_15_GC_PERCENT=43.478
PRIMER_RIGHT_15_GC_PERCENT=43.478
PRIMER_LEFT_15_SELF_ANY=5.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=2.00
PRIMER_LEFT_15_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_15_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_15_MISPRIMING_SCORE=23.00, reverse MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_LEFT_15_END_STABILITY=6.7000
PRIMER_RIGHT_15_END_STABILITY=7.9000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_15=635
PRIMER_PAIR_PENALTY_16=0.3887
PRIMER_LEFT_16_PENALTY=0.047652
PRIMER_RIGHT_16_PENALTY=0.341061
PRIMER_LEFT_16_SEQUENCE=TGAAGAATTATGGAGAGAACCCA
PRIMER_RIGHT_16_SEQUENCE=TTCTTGGCAGAATTGTACTTTCC
PRIMER_LEFT_16=152,23
PRIMER_RIGHT_16=1001,23
PRIMER_LEFT_16_TM=59.952
PRIMER_RIGHT_16_TM=59.659
PRIMER_LEFT_16_GC_PERCENT=39.130
PRIMER_RIGHT_16_GC_PERCENT=39.130
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=5.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=2.00
PRIMER_LEFT_16_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_16_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_16_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_16_END_STABILITY=9.4000
PRIMER_RIGHT_16_END_STABILITY=8.5000
PRIMER_PAIR_16_COMPL_ANY=6.00
PRIMER_PAIR_16_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_16=850
PRIMER_PAIR_PENALTY_17=0.3914
PRIMER_LEFT_17_PENALTY=0.073619
PRIMER_RIGHT_17_PENALTY=0.317733
PRIMER_LEFT_17_SEQUENCE=GAGCAGGCCTGTATTCTCTACAA
PRIMER_RIGHT_17_SEQUENCE=AAATGAAGTCGTTGTCCTTCTTG
PRIMER_LEFT_17=397,23
PRIMER_RIGHT_17=1018,23
PRIMER_LEFT_17_TM=59.926
PRIMER_RIGHT_17_TM=59.682
PRIMER_LEFT_17_GC_PERCENT=47.826
PRIMER_RIGHT_17_GC_PERCENT=39.130
PRIMER_LEFT_17_SELF_ANY=8.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=2.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_17_MISPRIMING_SCORE=12.00, L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_PAIR_17_MISPRIMING_SCORE=23.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_17_END_STABILITY=6.0000
PRIMER_RIGHT_17_END_STABILITY=7.0000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_17=622
PRIMER_PAIR_PENALTY_18=0.3971
PRIMER_LEFT_18_PENALTY=0.047652
PRIMER_RIGHT_18_PENALTY=0.349469
PRIMER_LEFT_18_SEQUENCE=TGAAGAATTATGGAGAGAACCCA
PRIMER_RIGHT_18_SEQUENCE=AGTTTCTTCCAGTCCTTCTGGAT
PRIMER_LEFT_18=152,23
PRIMER_RIGHT_18=779,23
PRIMER_LEFT_18_TM=59.952
PRIMER_RIGHT_18_TM=59.651
PRIMER_LEFT_18_GC_PERCENT=39.130
PRIMER_RIGHT_18_GC_PERCENT=43.478
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=5.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=3.00
PRIMER_LEFT_18_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_18_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_18_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_18_END_STABILITY=9.4000
PRIMER_RIGHT_18_END_STABILITY=8.1000
PRIMER_PAIR_18_COMPL_ANY=7.00
PRIMER_PAIR_18_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_18=628
PRIMER_PAIR_PENALTY_19=0.4074
PRIMER_LEFT_19_PENALTY=0.081398
PRIMER_RIGHT_19_PENALTY=0.326043
PRIMER_LEFT_19_SEQUENCE=CTGTCAGGAACCTTGTACAGTCC
PRIMER_RIGHT_19_SEQUENCE=CTCTCCAGATCTGCGTAGAAGTC
PRIMER_LEFT_19=1361,23
PRIMER_RIGHT_19=2111,23
PRIMER_LEFT_19_TM=60.081
PRIMER_RIGHT_19_TM=59.674
PRIMER_LEFT_19_GC_PERCENT=52.174
PRIMER_RIGHT_19_GC_PERCENT=52.174
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=8.00
PRIMER_LEFT_19_SELF_END=3.00
PRIMER_RIGHT_19_SELF_END=2.00
PRIMER_LEFT_19_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_19_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_19_MISPRIMING_SCORE=24.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_19_END_STABILITY=7.6000
PRIMER_RIGHT_19_END_STABILITY=6.4000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_19=751
=