PRIMER_SEQUENCE_ID=NM_016086
SEQUENCE=GCCACTTCCGGGAGTCGGAAAGGAAAGCTGTGGGACCATCCTGGCAACCCCGGTGTTTGGCTGGGTTCTAGCGTAGCCGTCTGTGTGGCCGGTGGGGGACCTGCGGTCGGAGTGGGAGGGCCAGTCTGCACCCAAGAGGTGGAAGAGGACGGGCTTTAGGCTGGAAGCGCCTTAGAGGAGCCATTTTTCCAGGTGGGGCCCCAGGCAGAGGCTCCGACAGGGAGCCTGGCCATAGTCGCGCAGCCGGGGAGGTGGAGCGCGTCCCAGACCCGAGCCCCCGACCTCAGCCAAACCCATTCCTTCTGCCCTTGGAGGCCAGAGGGGACTCTGAGCTCCGGAAAGGATGCCTGGTTTGCTTTTATGTGAACCAACAGAGCTTTACAACATCCTGAATCAGGCCACAAAACTCTCCAGATTAACAGACCCCAACTATCTCTGTTTATTGGATGTCCGTTCCAAATGGGAGTATGACGAAAGCCATGTGATCACTGCCCTTCGAGTGAAGAAGAAAAATAATGAATATCTTCTCCCGGAGTCTGTGGACCTGGAGTGTGTGAAGTACTGCGTGGTGTATGATAACAACAGCAGCACCCTGGAGATACTCTTAAAAGATGATGATGATGATTCAGACTCTGATGGTGATGGCAAAGATCTTGTGCCTCAAGCAGCCATTGAGTATGGCAGGATCCTGACCCGCCTCACCCACCACCCCGTCTACATCCTGAAAGGGGGCTATGAGCGCTTCTCAGGCACGTACCACTTTCTCCGGACCCAGAAGATCATCTGGATGCCTCAGGAACTGGATGCATTTCAGCCATACCCCATTGAAATCGTGCCAGGGAAGGTCTTCGTTGGCAATTTCAGTCAAGCCTGTGACCCCAAGATTCAGAAGGACTTGAAAATCAAAGCCCATGTCAATGTCTCCATGGATACAGGGCCCTTTTTTGCAGGCGATGCTGACAAGCTTCTGCACATCCGGATAGAAGATTCCCCGGAAGCCCAGATTCTTCCCTTCTTACGCCACATGTGTCACTTCATTGAAATTCACCATCACCTTGGCTCTGTCATTCTGATCTTTTCCACCCAAGGTATCAGCCGCAGTTGTGCCGCCATCATAGCCTACCTCATGCATAGTAACGAGCAGACCTTGCAGAGGTCCTGGGCCTATGTCAAGAAGTGCAAAAACAACATGTGTCCAAATCGGGGATTGGTGAGCCAGCTGCTGGAATGGGAGAAGACTATCCTTGGAGATTCCATCACAAACATCATGGATCCGCTCTACTGATCTTCTCCGAGGCCCACCGAAGGGTACTGAAGAGCCTCACCTGGGGGCATTTTGTGGGTGGAGGGCCAGAGTGTGTATACCCAGGCTTGTCTGGAAGGAGAAGGCCTTTGCTGCCTGAAAGTCTCATGTTAAAAAAAAAAAAAA
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 15795, GC content failed 224, low tm 5091, high tm 6908, high any compl 6, high end compl 9, high repeat similarity 19, long poly-x seq 21, ok 3517
PRIMER_RIGHT_EXPLAIN=considered 15728, GC content failed 251, low tm 5209, high tm 6669, high any compl 5, high end compl 8, high repeat similarity 4, long poly-x seq 34, ok 3548
PRIMER_PAIR_EXPLAIN=considered 807, unacceptable product size 769, ok 38
PRIMER_PAIR_PENALTY=0.0445
PRIMER_LEFT_PENALTY=0.019083
PRIMER_RIGHT_PENALTY=0.025403
PRIMER_LEFT_SEQUENCE=GAATATCTTCTCCCGGAGTCTGT
PRIMER_RIGHT_SEQUENCE=CTCCAAGGATAGTCTTCTCCCAT
PRIMER_LEFT=517,23
PRIMER_RIGHT=1249,23
PRIMER_LEFT_TM=59.981
PRIMER_RIGHT_TM=59.975
PRIMER_LEFT_GC_PERCENT=47.826
PRIMER_RIGHT_GC_PERCENT=47.826
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_MISPRIMING_SCORE=11.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_MISPRIMING_SCORE=12.00, MER42a MER42 repetitive sequence: subfamily MER42a - a consensus
PRIMER_PAIR_MISPRIMING_SCORE=20.00, MER42a MER42 repetitive sequence: subfamily MER42a - a consensus
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=9.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=733
PRIMER_PAIR_PENALTY_1=0.1197
PRIMER_LEFT_1_PENALTY=0.019083
PRIMER_RIGHT_1_PENALTY=0.100593
PRIMER_LEFT_1_SEQUENCE=GAATATCTTCTCCCGGAGTCTGT
PRIMER_RIGHT_1_SEQUENCE=AAGATCAGAATGACAGAGCCAAG
PRIMER_LEFT_1=517,23
PRIMER_RIGHT_1=1076,23
PRIMER_LEFT_1_TM=59.981
PRIMER_RIGHT_1_TM=59.899
PRIMER_LEFT_1_GC_PERCENT=47.826
PRIMER_RIGHT_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_MISPRIMING_SCORE=11.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_1_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=560
PRIMER_PAIR_PENALTY_2=0.1260
PRIMER_LEFT_2_PENALTY=0.100593
PRIMER_RIGHT_2_PENALTY=0.025403
PRIMER_LEFT_2_SEQUENCE=CTTGGCTCTGTCATTCTGATCTT
PRIMER_RIGHT_2_SEQUENCE=CTCCAAGGATAGTCTTCTCCCAT
PRIMER_LEFT_2=1054,23
PRIMER_RIGHT_2=1249,23
PRIMER_LEFT_2_TM=59.899
PRIMER_RIGHT_2_TM=59.975
PRIMER_LEFT_2_GC_PERCENT=43.478
PRIMER_RIGHT_2_GC_PERCENT=47.826
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_2_MISPRIMING_SCORE=12.00, MER42a MER42 repetitive sequence: subfamily MER42a - a consensus
PRIMER_PAIR_2_MISPRIMING_SCORE=21.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=9.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=196
PRIMER_PAIR_PENALTY_3=0.1379
PRIMER_LEFT_3_PENALTY=0.112479
PRIMER_RIGHT_3_PENALTY=0.025403
PRIMER_LEFT_3_SEQUENCE=AACTCTCCAGATTAACAGACCCC
PRIMER_RIGHT_3_SEQUENCE=CTCCAAGGATAGTCTTCTCCCAT
PRIMER_LEFT_3=404,23
PRIMER_RIGHT_3=1249,23
PRIMER_LEFT_3_TM=59.888
PRIMER_RIGHT_3_TM=59.975
PRIMER_LEFT_3_GC_PERCENT=47.826
PRIMER_RIGHT_3_GC_PERCENT=47.826
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, reverse MLT2C2 Interspersed repeat MLT2C2 - a consensus
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, MER42a MER42 repetitive sequence: subfamily MER42a - a consensus
PRIMER_PAIR_3_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_3_END_STABILITY=10.6000
PRIMER_RIGHT_3_END_STABILITY=9.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=846
PRIMER_PAIR_PENALTY_4=0.1402
PRIMER_LEFT_4_PENALTY=0.019083
PRIMER_RIGHT_4_PENALTY=0.121114
PRIMER_LEFT_4_SEQUENCE=GAATATCTTCTCCCGGAGTCTGT
PRIMER_RIGHT_4_SEQUENCE=GGCTTTGATTTTCAAGTCCTTCT
PRIMER_LEFT_4=517,23
PRIMER_RIGHT_4=909,23
PRIMER_LEFT_4_TM=59.981
PRIMER_RIGHT_4_TM=60.121
PRIMER_LEFT_4_GC_PERCENT=47.826
PRIMER_RIGHT_4_GC_PERCENT=39.130
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_MISPRIMING_SCORE=11.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, reverse PAB Human pseudoautosomal boundary-like sequence.
PRIMER_PAIR_4_MISPRIMING_SCORE=21.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=393
PRIMER_PAIR_PENALTY_5=0.1405
PRIMER_LEFT_5_PENALTY=0.115144
PRIMER_RIGHT_5_PENALTY=0.025403
PRIMER_LEFT_5_SEQUENCE=CTCTGTTTATTGGATGTCCGTTC
PRIMER_RIGHT_5_SEQUENCE=CTCCAAGGATAGTCTTCTCCCAT
PRIMER_LEFT_5=433,23
PRIMER_RIGHT_5=1249,23
PRIMER_LEFT_5_TM=59.885
PRIMER_RIGHT_5_TM=59.975
PRIMER_LEFT_5_GC_PERCENT=43.478
PRIMER_RIGHT_5_GC_PERCENT=47.826
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=2.00
PRIMER_LEFT_5_MISPRIMING_SCORE=12.00, reverse MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_RIGHT_5_MISPRIMING_SCORE=12.00, MER42a MER42 repetitive sequence: subfamily MER42a - a consensus
PRIMER_PAIR_5_MISPRIMING_SCORE=21.00, reverse MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_LEFT_5_END_STABILITY=8.4000
PRIMER_RIGHT_5_END_STABILITY=9.6000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_5=817
PRIMER_PAIR_PENALTY_6=0.1466
PRIMER_LEFT_6_PENALTY=0.121175
PRIMER_RIGHT_6_PENALTY=0.025403
PRIMER_LEFT_6_SEQUENCE=TCCAGATTAACAGACCCCAACTA
PRIMER_RIGHT_6_SEQUENCE=CTCCAAGGATAGTCTTCTCCCAT
PRIMER_LEFT_6=409,23
PRIMER_RIGHT_6=1249,23
PRIMER_LEFT_6_TM=59.879
PRIMER_RIGHT_6_TM=59.975
PRIMER_LEFT_6_GC_PERCENT=43.478
PRIMER_RIGHT_6_GC_PERCENT=47.826
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=2.00
PRIMER_RIGHT_6_SELF_END=2.00
PRIMER_LEFT_6_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_6_MISPRIMING_SCORE=12.00, MER42a MER42 repetitive sequence: subfamily MER42a - a consensus
PRIMER_PAIR_6_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_6_END_STABILITY=5.7000
PRIMER_RIGHT_6_END_STABILITY=9.6000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_6=841
PRIMER_PAIR_PENALTY_7=0.1892
PRIMER_LEFT_7_PENALTY=0.163755
PRIMER_RIGHT_7_PENALTY=0.025403
PRIMER_LEFT_7_SEQUENCE=TGTGAACCAACAGAGCTTTACAA
PRIMER_RIGHT_7_SEQUENCE=CTCCAAGGATAGTCTTCTCCCAT
PRIMER_LEFT_7=361,23
PRIMER_RIGHT_7=1249,23
PRIMER_LEFT_7_TM=59.836
PRIMER_RIGHT_7_TM=59.975
PRIMER_LEFT_7_GC_PERCENT=39.130
PRIMER_RIGHT_7_GC_PERCENT=47.826
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=2.00
PRIMER_RIGHT_7_SELF_END=2.00
PRIMER_LEFT_7_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_7_MISPRIMING_SCORE=12.00, MER42a MER42 repetitive sequence: subfamily MER42a - a consensus
PRIMER_PAIR_7_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_7_END_STABILITY=6.0000
PRIMER_RIGHT_7_END_STABILITY=9.6000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_7=889
PRIMER_PAIR_PENALTY_8=0.2007
PRIMER_LEFT_8_PENALTY=0.175264
PRIMER_RIGHT_8_PENALTY=0.025403
PRIMER_LEFT_8_SEQUENCE=AATGAATATCTTCTCCCGGAGTC
PRIMER_RIGHT_8_SEQUENCE=CTCCAAGGATAGTCTTCTCCCAT
PRIMER_LEFT_8=514,23
PRIMER_RIGHT_8=1249,23
PRIMER_LEFT_8_TM=59.825
PRIMER_RIGHT_8_TM=59.975
PRIMER_LEFT_8_GC_PERCENT=43.478
PRIMER_RIGHT_8_GC_PERCENT=47.826
PRIMER_LEFT_8_SELF_ANY=7.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=3.00
PRIMER_RIGHT_8_SELF_END=2.00
PRIMER_LEFT_8_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, MER42a MER42 repetitive sequence: subfamily MER42a - a consensus
PRIMER_PAIR_8_MISPRIMING_SCORE=21.00, MER42a MER42 repetitive sequence: subfamily MER42a - a consensus
PRIMER_LEFT_8_END_STABILITY=6.1000
PRIMER_RIGHT_8_END_STABILITY=9.6000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_8=736
PRIMER_PAIR_PENALTY_9=0.2131
PRIMER_LEFT_9_PENALTY=0.112479
PRIMER_RIGHT_9_PENALTY=0.100593
PRIMER_LEFT_9_SEQUENCE=AACTCTCCAGATTAACAGACCCC
PRIMER_RIGHT_9_SEQUENCE=AAGATCAGAATGACAGAGCCAAG
PRIMER_LEFT_9=404,23
PRIMER_RIGHT_9=1076,23
PRIMER_LEFT_9_TM=59.888
PRIMER_RIGHT_9_TM=59.899
PRIMER_LEFT_9_GC_PERCENT=47.826
PRIMER_RIGHT_9_GC_PERCENT=43.478
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_MISPRIMING_SCORE=12.00, reverse MLT2C2 Interspersed repeat MLT2C2 - a consensus
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_9_MISPRIMING_SCORE=23.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_9_END_STABILITY=10.6000
PRIMER_RIGHT_9_END_STABILITY=8.5000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_9=673
PRIMER_PAIR_PENALTY_10=0.2157
PRIMER_LEFT_10_PENALTY=0.115144
PRIMER_RIGHT_10_PENALTY=0.100593
PRIMER_LEFT_10_SEQUENCE=CTCTGTTTATTGGATGTCCGTTC
PRIMER_RIGHT_10_SEQUENCE=AAGATCAGAATGACAGAGCCAAG
PRIMER_LEFT_10=433,23
PRIMER_RIGHT_10=1076,23
PRIMER_LEFT_10_TM=59.885
PRIMER_RIGHT_10_TM=59.899
PRIMER_LEFT_10_GC_PERCENT=43.478
PRIMER_RIGHT_10_GC_PERCENT=43.478
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_MISPRIMING_SCORE=12.00, reverse MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_RIGHT_10_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_10_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_10_END_STABILITY=8.4000
PRIMER_RIGHT_10_END_STABILITY=8.5000
PRIMER_PAIR_10_COMPL_ANY=6.00
PRIMER_PAIR_10_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_10=644
PRIMER_PAIR_PENALTY_11=0.2193
PRIMER_LEFT_11_PENALTY=0.193896
PRIMER_RIGHT_11_PENALTY=0.025403
PRIMER_LEFT_11_SEQUENCE=GGTTTGCTTTTATGTGAACCAAC
PRIMER_RIGHT_11_SEQUENCE=CTCCAAGGATAGTCTTCTCCCAT
PRIMER_LEFT_11=349,23
PRIMER_RIGHT_11=1249,23
PRIMER_LEFT_11_TM=59.806
PRIMER_RIGHT_11_TM=59.975
PRIMER_LEFT_11_GC_PERCENT=39.130
PRIMER_RIGHT_11_GC_PERCENT=47.826
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=2.00
PRIMER_RIGHT_11_SELF_END=2.00
PRIMER_LEFT_11_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, MER42a MER42 repetitive sequence: subfamily MER42a - a consensus
PRIMER_PAIR_11_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_11_END_STABILITY=8.2000
PRIMER_RIGHT_11_END_STABILITY=9.6000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_11=901
PRIMER_PAIR_PENALTY_12=0.2218
PRIMER_LEFT_12_PENALTY=0.121175
PRIMER_RIGHT_12_PENALTY=0.100593
PRIMER_LEFT_12_SEQUENCE=TCCAGATTAACAGACCCCAACTA
PRIMER_RIGHT_12_SEQUENCE=AAGATCAGAATGACAGAGCCAAG
PRIMER_LEFT_12=409,23
PRIMER_RIGHT_12=1076,23
PRIMER_LEFT_12_TM=59.879
PRIMER_RIGHT_12_TM=59.899
PRIMER_LEFT_12_GC_PERCENT=43.478
PRIMER_RIGHT_12_GC_PERCENT=43.478
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=2.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_12_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_12_MISPRIMING_SCORE=24.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_12_END_STABILITY=5.7000
PRIMER_RIGHT_12_END_STABILITY=8.5000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_12=668
PRIMER_PAIR_PENALTY_13=0.2226
PRIMER_LEFT_13_PENALTY=0.112479
PRIMER_RIGHT_13_PENALTY=0.110131
PRIMER_LEFT_13_SEQUENCE=AACTCTCCAGATTAACAGACCCC
PRIMER_RIGHT_13_SEQUENCE=TCATACACCACGCAGTACTTCAC
PRIMER_LEFT_13=404,23
PRIMER_RIGHT_13=575,23
PRIMER_LEFT_13_TM=59.888
PRIMER_RIGHT_13_TM=60.110
PRIMER_LEFT_13_GC_PERCENT=47.826
PRIMER_RIGHT_13_GC_PERCENT=47.826
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=6.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=2.00
PRIMER_LEFT_13_MISPRIMING_SCORE=12.00, reverse MLT2C2 Interspersed repeat MLT2C2 - a consensus
PRIMER_RIGHT_13_MISPRIMING_SCORE=11.00, MER6 Nonautonomous DNA transposon
PRIMER_PAIR_13_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_13_END_STABILITY=10.6000
PRIMER_RIGHT_13_END_STABILITY=6.7000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_13=172
PRIMER_PAIR_PENALTY_14=0.2253
PRIMER_LEFT_14_PENALTY=0.115144
PRIMER_RIGHT_14_PENALTY=0.110131
PRIMER_LEFT_14_SEQUENCE=CTCTGTTTATTGGATGTCCGTTC
PRIMER_RIGHT_14_SEQUENCE=TCATACACCACGCAGTACTTCAC
PRIMER_LEFT_14=433,23
PRIMER_RIGHT_14=575,23
PRIMER_LEFT_14_TM=59.885
PRIMER_RIGHT_14_TM=60.110
PRIMER_LEFT_14_GC_PERCENT=43.478
PRIMER_RIGHT_14_GC_PERCENT=47.826
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=2.00
PRIMER_LEFT_14_MISPRIMING_SCORE=12.00, reverse MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_RIGHT_14_MISPRIMING_SCORE=11.00, MER6 Nonautonomous DNA transposon
PRIMER_PAIR_14_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_14_END_STABILITY=8.4000
PRIMER_RIGHT_14_END_STABILITY=6.7000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_14=143
PRIMER_PAIR_PENALTY_15=0.2271
PRIMER_LEFT_15_PENALTY=0.112479
PRIMER_RIGHT_15_PENALTY=0.114572
PRIMER_LEFT_15_SEQUENCE=AACTCTCCAGATTAACAGACCCC
PRIMER_RIGHT_15_SEQUENCE=ATTTTTCTTCTTCACTCGAAGGG
PRIMER_LEFT_15=404,23
PRIMER_RIGHT_15=513,23
PRIMER_LEFT_15_TM=59.888
PRIMER_RIGHT_15_TM=60.115
PRIMER_LEFT_15_GC_PERCENT=47.826
PRIMER_RIGHT_15_GC_PERCENT=39.130
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=5.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=2.00
PRIMER_LEFT_15_MISPRIMING_SCORE=12.00, reverse MLT2C2 Interspersed repeat MLT2C2 - a consensus
PRIMER_RIGHT_15_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_15_MISPRIMING_SCORE=23.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_15_END_STABILITY=10.6000
PRIMER_RIGHT_15_END_STABILITY=9.7000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_15=110
PRIMER_PAIR_PENALTY_16=0.2313
PRIMER_LEFT_16_PENALTY=0.121175
PRIMER_RIGHT_16_PENALTY=0.110131
PRIMER_LEFT_16_SEQUENCE=TCCAGATTAACAGACCCCAACTA
PRIMER_RIGHT_16_SEQUENCE=TCATACACCACGCAGTACTTCAC
PRIMER_LEFT_16=409,23
PRIMER_RIGHT_16=575,23
PRIMER_LEFT_16_TM=59.879
PRIMER_RIGHT_16_TM=60.110
PRIMER_LEFT_16_GC_PERCENT=43.478
PRIMER_RIGHT_16_GC_PERCENT=47.826
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=6.00
PRIMER_LEFT_16_SELF_END=2.00
PRIMER_RIGHT_16_SELF_END=2.00
PRIMER_LEFT_16_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_16_MISPRIMING_SCORE=11.00, MER6 Nonautonomous DNA transposon
PRIMER_PAIR_16_MISPRIMING_SCORE=21.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_16_END_STABILITY=5.7000
PRIMER_RIGHT_16_END_STABILITY=6.7000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_16=167
PRIMER_PAIR_PENALTY_17=0.2336
PRIMER_LEFT_17_PENALTY=0.112479
PRIMER_RIGHT_17_PENALTY=0.121114
PRIMER_LEFT_17_SEQUENCE=AACTCTCCAGATTAACAGACCCC
PRIMER_RIGHT_17_SEQUENCE=GGCTTTGATTTTCAAGTCCTTCT
PRIMER_LEFT_17=404,23
PRIMER_RIGHT_17=909,23
PRIMER_LEFT_17_TM=59.888
PRIMER_RIGHT_17_TM=60.121
PRIMER_LEFT_17_GC_PERCENT=47.826
PRIMER_RIGHT_17_GC_PERCENT=39.130
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=5.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_MISPRIMING_SCORE=12.00, reverse MLT2C2 Interspersed repeat MLT2C2 - a consensus
PRIMER_RIGHT_17_MISPRIMING_SCORE=12.00, reverse PAB Human pseudoautosomal boundary-like sequence.
PRIMER_PAIR_17_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_17_END_STABILITY=10.6000
PRIMER_RIGHT_17_END_STABILITY=6.7000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_17=506
PRIMER_PAIR_PENALTY_18=0.2357
PRIMER_LEFT_18_PENALTY=0.121175
PRIMER_RIGHT_18_PENALTY=0.114572
PRIMER_LEFT_18_SEQUENCE=TCCAGATTAACAGACCCCAACTA
PRIMER_RIGHT_18_SEQUENCE=ATTTTTCTTCTTCACTCGAAGGG
PRIMER_LEFT_18=409,23
PRIMER_RIGHT_18=513,23
PRIMER_LEFT_18_TM=59.879
PRIMER_RIGHT_18_TM=60.115
PRIMER_LEFT_18_GC_PERCENT=43.478
PRIMER_RIGHT_18_GC_PERCENT=39.130
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=5.00
PRIMER_LEFT_18_SELF_END=2.00
PRIMER_RIGHT_18_SELF_END=2.00
PRIMER_LEFT_18_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_18_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_18_MISPRIMING_SCORE=24.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_18_END_STABILITY=5.7000
PRIMER_RIGHT_18_END_STABILITY=9.7000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_18=105
PRIMER_PAIR_PENALTY_19=0.2363
PRIMER_LEFT_19_PENALTY=0.115144
PRIMER_RIGHT_19_PENALTY=0.121114
PRIMER_LEFT_19_SEQUENCE=CTCTGTTTATTGGATGTCCGTTC
PRIMER_RIGHT_19_SEQUENCE=GGCTTTGATTTTCAAGTCCTTCT
PRIMER_LEFT_19=433,23
PRIMER_RIGHT_19=909,23
PRIMER_LEFT_19_TM=59.885
PRIMER_RIGHT_19_TM=60.121
PRIMER_LEFT_19_GC_PERCENT=43.478
PRIMER_RIGHT_19_GC_PERCENT=39.130
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=5.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_MISPRIMING_SCORE=12.00, reverse MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_RIGHT_19_MISPRIMING_SCORE=12.00, reverse PAB Human pseudoautosomal boundary-like sequence.
PRIMER_PAIR_19_MISPRIMING_SCORE=20.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_19_END_STABILITY=8.4000
PRIMER_RIGHT_19_END_STABILITY=6.7000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_19=477
=