PRIMER_SEQUENCE_ID=NM_017823
SEQUENCE=GTGGCCCGGGAGGCGCCGAGGCCAGGTAGGTGCGATGGGCGTGCAGCCCCCCAACTTCTCCTGGGTGCTTCCGGGCCGGCTGGCGGGACTGGCGCTGCCGCGGCTCCCCGCCCACTACCAGTTCCTGTTGGACCTGGGCGTGCGGCACCTGGTGTCCCTGACGGAGCGCGGGCCCCCTCACAGCGACAGCTGCCCCGGCCTCACCCTGCACCGCCTGCGCATCCCCGACTTCTGCCCGCCGGCCCCCGACCAGATCGACCGCTTCGTGCAGATCGTGGACGAGGCCAACGCACGGGGAGAGGCTGTGGGAGTGCACTGTGCTCTGGGCTTTGGCCGCACTGGCACCATGCTGGCCTGTTACCTGGTGAAGGAGCGGGGCTTGGCTGCAGGAGATGCCATTGCTGAAATCCGACGACTACGACCCGGCCCCATCGAGACCTATGAGCAGGAGAAAGCAGTCTTCCAGTTCTACCAGCGAACGAAATAAGGGGCCTTAGTACCCTTCTACCAGGCCCTCACTCCCCTTCCCCATGTTGTCGATGGGGCCAGAGATGAAGGGAAGTGGACTAAAGTATTAAACCCTCTAGCTCCCATTGGCTGAAGACACTGAAGTAGCCCACCCCTGCAGGCAGGTCCTGATTGAAGGGGAGGCTTGTACTGCTTTGTTGAATAAATGAGTTTTACGAACCAGGGAAAAAAAAAAAAAAAAAAAA
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 7206, GC content failed 706, low tm 1374, high tm 4048, high any compl 9, high end compl 16, high repeat similarity 24, long poly-x seq 21, ok 1008
PRIMER_RIGHT_EXPLAIN=considered 7242, GC content failed 513, low tm 1685, high tm 3857, high any compl 2, high end compl 2, high repeat similarity 17, long poly-x seq 18, ok 1148
PRIMER_PAIR_EXPLAIN=considered 551, unacceptable product size 518, high end compl 3, ok 30
PRIMER_PAIR_PENALTY=0.6558
PRIMER_LEFT_PENALTY=0.536322
PRIMER_RIGHT_PENALTY=0.119476
PRIMER_LEFT_SEQUENCE=AAAGCAGTCTTCCAGTTCTACCA
PRIMER_RIGHT_SEQUENCE=CAATGGGAGCTAGAGGGTTTAAT
PRIMER_LEFT=451,23
PRIMER_RIGHT=595,23
PRIMER_LEFT_TM=59.464
PRIMER_RIGHT_TM=59.881
PRIMER_LEFT_GC_PERCENT=43.478
PRIMER_RIGHT_GC_PERCENT=43.478
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_MISPRIMING_SCORE=12.00, LTR9 LTR from human endogenous retrovirus-like sequence (HUERS-P3)
PRIMER_PAIR_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=6.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=145
PRIMER_PAIR_PENALTY_1=0.7579
PRIMER_LEFT_1_PENALTY=0.638394
PRIMER_RIGHT_1_PENALTY=0.119476
PRIMER_LEFT_1_SEQUENCE=AGTTCTACCAGCGAACGAAATAA
PRIMER_RIGHT_1_SEQUENCE=CAATGGGAGCTAGAGGGTTTAAT
PRIMER_LEFT_1=464,23
PRIMER_RIGHT_1=595,23
PRIMER_LEFT_1_TM=59.362
PRIMER_RIGHT_1_TM=59.881
PRIMER_LEFT_1_GC_PERCENT=39.130
PRIMER_RIGHT_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, LTR9 LTR from human endogenous retrovirus-like sequence (HUERS-P3)
PRIMER_PAIR_1_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_1_END_STABILITY=6.2000
PRIMER_RIGHT_1_END_STABILITY=6.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=132
PRIMER_PAIR_PENALTY_2=0.7771
PRIMER_LEFT_2_PENALTY=0.536322
PRIMER_RIGHT_2_PENALTY=0.240778
PRIMER_LEFT_2_SEQUENCE=AAAGCAGTCTTCCAGTTCTACCA
PRIMER_RIGHT_2_SEQUENCE=TAGTCCACTTCCCTTCATCTCTG
PRIMER_LEFT_2=451,23
PRIMER_RIGHT_2=568,23
PRIMER_LEFT_2_TM=59.464
PRIMER_RIGHT_2_TM=59.759
PRIMER_LEFT_2_GC_PERCENT=43.478
PRIMER_RIGHT_2_GC_PERCENT=47.826
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_2_MISPRIMING_SCORE=11.00, MER4B a consensus
PRIMER_PAIR_2_MISPRIMING_SCORE=21.00, reverse SVA Composite retroposon
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=118
PRIMER_PAIR_PENALTY_3=0.8792
PRIMER_LEFT_3_PENALTY=0.638394
PRIMER_RIGHT_3_PENALTY=0.240778
PRIMER_LEFT_3_SEQUENCE=AGTTCTACCAGCGAACGAAATAA
PRIMER_RIGHT_3_SEQUENCE=TAGTCCACTTCCCTTCATCTCTG
PRIMER_LEFT_3=464,23
PRIMER_RIGHT_3=568,23
PRIMER_LEFT_3_TM=59.362
PRIMER_RIGHT_3_TM=59.759
PRIMER_LEFT_3_GC_PERCENT=39.130
PRIMER_RIGHT_3_GC_PERCENT=47.826
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_3_MISPRIMING_SCORE=11.00, MER4B a consensus
PRIMER_PAIR_3_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_3_END_STABILITY=6.2000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=105
PRIMER_PAIR_PENALTY_4=1.0089
PRIMER_LEFT_4_PENALTY=0.889386
PRIMER_RIGHT_4_PENALTY=0.119476
PRIMER_LEFT_4_SEQUENCE=AAGCAGTCTTCCAGTTCTACCAG
PRIMER_RIGHT_4_SEQUENCE=CAATGGGAGCTAGAGGGTTTAAT
PRIMER_LEFT_4=452,23
PRIMER_RIGHT_4=595,23
PRIMER_LEFT_4_TM=59.111
PRIMER_RIGHT_4_TM=59.881
PRIMER_LEFT_4_GC_PERCENT=47.826
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, reverse L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, LTR9 LTR from human endogenous retrovirus-like sequence (HUERS-P3)
PRIMER_PAIR_4_MISPRIMING_SCORE=21.00, LTR2 LTR from human endogenous retrovirus (4-14), 3' long terminal repeat
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=6.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=144
PRIMER_PAIR_PENALTY_5=1.1302
PRIMER_LEFT_5_PENALTY=0.889386
PRIMER_RIGHT_5_PENALTY=0.240778
PRIMER_LEFT_5_SEQUENCE=AAGCAGTCTTCCAGTTCTACCAG
PRIMER_RIGHT_5_SEQUENCE=TAGTCCACTTCCCTTCATCTCTG
PRIMER_LEFT_5=452,23
PRIMER_RIGHT_5=568,23
PRIMER_LEFT_5_TM=59.111
PRIMER_RIGHT_5_TM=59.759
PRIMER_LEFT_5_GC_PERCENT=47.826
PRIMER_RIGHT_5_GC_PERCENT=47.826
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_MISPRIMING_SCORE=12.00, reverse L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence
PRIMER_RIGHT_5_MISPRIMING_SCORE=11.00, MER4B a consensus
PRIMER_PAIR_5_MISPRIMING_SCORE=20.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_5_END_STABILITY=7.9000
PRIMER_RIGHT_5_END_STABILITY=6.7000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_5=117
PRIMER_PAIR_PENALTY_6=1.4322
PRIMER_LEFT_6_PENALTY=1.312713
PRIMER_RIGHT_6_PENALTY=0.119476
PRIMER_LEFT_6_SEQUENCE=AGCAGTCTTCCAGTTCTACCAGC
PRIMER_RIGHT_6_SEQUENCE=CAATGGGAGCTAGAGGGTTTAAT
PRIMER_LEFT_6=453,23
PRIMER_RIGHT_6=595,23
PRIMER_LEFT_6_TM=61.313
PRIMER_RIGHT_6_TM=59.881
PRIMER_LEFT_6_GC_PERCENT=52.174
PRIMER_RIGHT_6_GC_PERCENT=43.478
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=2.00
PRIMER_RIGHT_6_SELF_END=2.00
PRIMER_LEFT_6_MISPRIMING_SCORE=12.00, LTR2 LTR from human endogenous retrovirus (4-14), 3' long terminal repeat
PRIMER_RIGHT_6_MISPRIMING_SCORE=12.00, LTR9 LTR from human endogenous retrovirus-like sequence (HUERS-P3)
PRIMER_PAIR_6_MISPRIMING_SCORE=22.00, LTR2 LTR from human endogenous retrovirus (4-14), 3' long terminal repeat
PRIMER_LEFT_6_END_STABILITY=9.7000
PRIMER_RIGHT_6_END_STABILITY=6.2000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_6=143
PRIMER_PAIR_PENALTY_7=1.4556
PRIMER_LEFT_7_PENALTY=1.336085
PRIMER_RIGHT_7_PENALTY=0.119476
PRIMER_LEFT_7_SEQUENCE=AAAGCAGTCTTCCAGTTCTACCAG
PRIMER_RIGHT_7_SEQUENCE=CAATGGGAGCTAGAGGGTTTAAT
PRIMER_LEFT_7=451,24
PRIMER_RIGHT_7=595,23
PRIMER_LEFT_7_TM=60.336
PRIMER_RIGHT_7_TM=59.881
PRIMER_LEFT_7_GC_PERCENT=45.833
PRIMER_RIGHT_7_GC_PERCENT=43.478
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=2.00
PRIMER_LEFT_7_MISPRIMING_SCORE=12.00, TAR1 Human telomere associated repeat sequence, complete sequence
PRIMER_RIGHT_7_MISPRIMING_SCORE=12.00, LTR9 LTR from human endogenous retrovirus-like sequence (HUERS-P3)
PRIMER_PAIR_7_MISPRIMING_SCORE=21.00, LTR2 LTR from human endogenous retrovirus (4-14), 3' long terminal repeat
PRIMER_LEFT_7_END_STABILITY=7.9000
PRIMER_RIGHT_7_END_STABILITY=6.2000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_7=145
PRIMER_PAIR_PENALTY_8=1.5440
PRIMER_LEFT_8_PENALTY=0.240778
PRIMER_RIGHT_8_PENALTY=1.303210
PRIMER_LEFT_8_SEQUENCE=CAGAGATGAAGGGAAGTGGACTA
PRIMER_RIGHT_8_SEQUENCE=CCCTGGTTCGTAAAACTCATTTAT
PRIMER_LEFT_8=546,23
PRIMER_RIGHT_8=692,24
PRIMER_LEFT_8_TM=59.759
PRIMER_RIGHT_8_TM=59.697
PRIMER_LEFT_8_GC_PERCENT=47.826
PRIMER_RIGHT_8_GC_PERCENT=37.500
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=2.00
PRIMER_RIGHT_8_SELF_END=3.00
PRIMER_LEFT_8_MISPRIMING_SCORE=11.00, LTR11 LTR from human HTLV-I related endogenous retroviral sequence (HRES-1/1)
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_8_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_8_END_STABILITY=5.4000
PRIMER_RIGHT_8_END_STABILITY=6.2000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_8=147
PRIMER_PAIR_PENALTY_9=1.5535
PRIMER_LEFT_9_PENALTY=1.312713
PRIMER_RIGHT_9_PENALTY=0.240778
PRIMER_LEFT_9_SEQUENCE=AGCAGTCTTCCAGTTCTACCAGC
PRIMER_RIGHT_9_SEQUENCE=TAGTCCACTTCCCTTCATCTCTG
PRIMER_LEFT_9=453,23
PRIMER_RIGHT_9=568,23
PRIMER_LEFT_9_TM=61.313
PRIMER_RIGHT_9_TM=59.759
PRIMER_LEFT_9_GC_PERCENT=52.174
PRIMER_RIGHT_9_GC_PERCENT=47.826
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=2.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_MISPRIMING_SCORE=12.00, LTR2 LTR from human endogenous retrovirus (4-14), 3' long terminal repeat
PRIMER_RIGHT_9_MISPRIMING_SCORE=11.00, MER4B a consensus
PRIMER_PAIR_9_MISPRIMING_SCORE=20.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_9_END_STABILITY=9.7000
PRIMER_RIGHT_9_END_STABILITY=6.7000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_9=116
PRIMER_PAIR_PENALTY_10=1.5552
PRIMER_LEFT_10_PENALTY=1.435722
PRIMER_RIGHT_10_PENALTY=0.119476
PRIMER_LEFT_10_SEQUENCE=AGTCTTCCAGTTCTACCAGCGA
PRIMER_RIGHT_10_SEQUENCE=CAATGGGAGCTAGAGGGTTTAAT
PRIMER_LEFT_10=456,22
PRIMER_RIGHT_10=595,23
PRIMER_LEFT_10_TM=60.436
PRIMER_RIGHT_10_TM=59.881
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=43.478
PRIMER_LEFT_10_SELF_ANY=3.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=2.00
PRIMER_LEFT_10_MISPRIMING_SCORE=12.00, LTR2 LTR from human endogenous retrovirus (4-14), 3' long terminal repeat
PRIMER_RIGHT_10_MISPRIMING_SCORE=12.00, LTR9 LTR from human endogenous retrovirus-like sequence (HUERS-P3)
PRIMER_PAIR_10_MISPRIMING_SCORE=22.00, LTR2 LTR from human endogenous retrovirus (4-14), 3' long terminal repeat
PRIMER_LEFT_10_END_STABILITY=9.9000
PRIMER_RIGHT_10_END_STABILITY=6.2000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_10=140
PRIMER_PAIR_PENALTY_11=1.5636
PRIMER_LEFT_11_PENALTY=1.444124
PRIMER_RIGHT_11_PENALTY=0.119476
PRIMER_LEFT_11_SEQUENCE=GCAGTCTTCCAGTTCTACCAGC
PRIMER_RIGHT_11_SEQUENCE=CAATGGGAGCTAGAGGGTTTAAT
PRIMER_LEFT_11=454,22
PRIMER_RIGHT_11=595,23
PRIMER_LEFT_11_TM=60.444
PRIMER_RIGHT_11_TM=59.881
PRIMER_LEFT_11_GC_PERCENT=54.545
PRIMER_RIGHT_11_GC_PERCENT=43.478
PRIMER_LEFT_11_SELF_ANY=3.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=2.00
PRIMER_RIGHT_11_SELF_END=2.00
PRIMER_LEFT_11_MISPRIMING_SCORE=12.00, LTR2 LTR from human endogenous retrovirus (4-14), 3' long terminal repeat
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, LTR9 LTR from human endogenous retrovirus-like sequence (HUERS-P3)
PRIMER_PAIR_11_MISPRIMING_SCORE=22.00, LTR2 LTR from human endogenous retrovirus (4-14), 3' long terminal repeat
PRIMER_LEFT_11_END_STABILITY=9.7000
PRIMER_RIGHT_11_END_STABILITY=6.2000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_11=142
PRIMER_PAIR_PENALTY_12=1.5769
PRIMER_LEFT_12_PENALTY=1.336085
PRIMER_RIGHT_12_PENALTY=0.240778
PRIMER_LEFT_12_SEQUENCE=AAAGCAGTCTTCCAGTTCTACCAG
PRIMER_RIGHT_12_SEQUENCE=TAGTCCACTTCCCTTCATCTCTG
PRIMER_LEFT_12=451,24
PRIMER_RIGHT_12=568,23
PRIMER_LEFT_12_TM=60.336
PRIMER_RIGHT_12_TM=59.759
PRIMER_LEFT_12_GC_PERCENT=45.833
PRIMER_RIGHT_12_GC_PERCENT=47.826
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_MISPRIMING_SCORE=12.00, TAR1 Human telomere associated repeat sequence, complete sequence
PRIMER_RIGHT_12_MISPRIMING_SCORE=11.00, MER4B a consensus
PRIMER_PAIR_12_MISPRIMING_SCORE=20.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_12_END_STABILITY=7.9000
PRIMER_RIGHT_12_END_STABILITY=6.7000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_12=118
PRIMER_PAIR_PENALTY_13=1.6134
PRIMER_LEFT_13_PENALTY=1.493971
PRIMER_RIGHT_13_PENALTY=0.119476
PRIMER_LEFT_13_SEQUENCE=GGGCCTTAGTACCCTTCTACCAG
PRIMER_RIGHT_13_SEQUENCE=CAATGGGAGCTAGAGGGTTTAAT
PRIMER_LEFT_13=488,23
PRIMER_RIGHT_13=595,23
PRIMER_LEFT_13_TM=61.494
PRIMER_RIGHT_13_TM=59.881
PRIMER_LEFT_13_GC_PERCENT=56.522
PRIMER_RIGHT_13_GC_PERCENT=43.478
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=2.00
PRIMER_RIGHT_13_SELF_END=2.00
PRIMER_LEFT_13_MISPRIMING_SCORE=11.00, THE1BR THE-1B Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_RIGHT_13_MISPRIMING_SCORE=12.00, LTR9 LTR from human endogenous retrovirus-like sequence (HUERS-P3)
PRIMER_PAIR_13_MISPRIMING_SCORE=20.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_13_END_STABILITY=7.9000
PRIMER_RIGHT_13_END_STABILITY=6.2000
PRIMER_PAIR_13_COMPL_ANY=6.00
PRIMER_PAIR_13_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_13=108
PRIMER_PAIR_PENALTY_14=1.6134
PRIMER_LEFT_14_PENALTY=1.493971
PRIMER_RIGHT_14_PENALTY=0.119476
PRIMER_LEFT_14_SEQUENCE=CTTAGTACCCTTCTACCAGGCCC
PRIMER_RIGHT_14_SEQUENCE=CAATGGGAGCTAGAGGGTTTAAT
PRIMER_LEFT_14=492,23
PRIMER_RIGHT_14=595,23
PRIMER_LEFT_14_TM=61.494
PRIMER_RIGHT_14_TM=59.881
PRIMER_LEFT_14_GC_PERCENT=56.522
PRIMER_RIGHT_14_GC_PERCENT=43.478
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=3.00
PRIMER_RIGHT_14_SELF_END=2.00
PRIMER_LEFT_14_MISPRIMING_SCORE=11.00, reverse MSTa consensus sequence
PRIMER_RIGHT_14_MISPRIMING_SCORE=12.00, LTR9 LTR from human endogenous retrovirus-like sequence (HUERS-P3)
PRIMER_PAIR_14_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_14_END_STABILITY=12.4000
PRIMER_RIGHT_14_END_STABILITY=6.2000
PRIMER_PAIR_14_COMPL_ANY=6.00
PRIMER_PAIR_14_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_14=104
PRIMER_PAIR_PENALTY_15=1.6305
PRIMER_LEFT_15_PENALTY=1.510985
PRIMER_RIGHT_15_PENALTY=0.119476
PRIMER_LEFT_15_SEQUENCE=ATTGCTGAAATCCGACGACTAC
PRIMER_RIGHT_15_SEQUENCE=CAATGGGAGCTAGAGGGTTTAAT
PRIMER_LEFT_15=397,22
PRIMER_RIGHT_15=595,23
PRIMER_LEFT_15_TM=60.511
PRIMER_RIGHT_15_TM=59.881
PRIMER_LEFT_15_GC_PERCENT=45.455
PRIMER_RIGHT_15_GC_PERCENT=43.478
PRIMER_LEFT_15_SELF_ANY=5.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=2.00
PRIMER_LEFT_15_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_15_MISPRIMING_SCORE=12.00, LTR9 LTR from human endogenous retrovirus-like sequence (HUERS-P3)
PRIMER_PAIR_15_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_15_END_STABILITY=5.1000
PRIMER_RIGHT_15_END_STABILITY=6.2000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_15=199
PRIMER_PAIR_PENALTY_16=1.6429
PRIMER_LEFT_16_PENALTY=0.536322
PRIMER_RIGHT_16_PENALTY=1.106607
PRIMER_LEFT_16_SEQUENCE=AAAGCAGTCTTCCAGTTCTACCA
PRIMER_RIGHT_16_SEQUENCE=CAATGGGAGCTAGAGGGTTTAATA
PRIMER_LEFT_16=451,23
PRIMER_RIGHT_16=595,24
PRIMER_LEFT_16_TM=59.464
PRIMER_RIGHT_16_TM=59.893
PRIMER_LEFT_16_GC_PERCENT=43.478
PRIMER_RIGHT_16_GC_PERCENT=41.667
PRIMER_LEFT_16_SELF_ANY=3.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=3.00
PRIMER_LEFT_16_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_16_MISPRIMING_SCORE=11.00, LTR9 LTR from human endogenous retrovirus-like sequence (HUERS-P3)
PRIMER_PAIR_16_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_16_END_STABILITY=7.2000
PRIMER_RIGHT_16_END_STABILITY=5.2000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_16=145
PRIMER_PAIR_PENALTY_17=1.6849
PRIMER_LEFT_17_PENALTY=1.444124
PRIMER_RIGHT_17_PENALTY=0.240778
PRIMER_LEFT_17_SEQUENCE=GCAGTCTTCCAGTTCTACCAGC
PRIMER_RIGHT_17_SEQUENCE=TAGTCCACTTCCCTTCATCTCTG
PRIMER_LEFT_17=454,22
PRIMER_RIGHT_17=568,23
PRIMER_LEFT_17_TM=60.444
PRIMER_RIGHT_17_TM=59.759
PRIMER_LEFT_17_GC_PERCENT=54.545
PRIMER_RIGHT_17_GC_PERCENT=47.826
PRIMER_LEFT_17_SELF_ANY=3.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=2.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_MISPRIMING_SCORE=12.00, LTR2 LTR from human endogenous retrovirus (4-14), 3' long terminal repeat
PRIMER_RIGHT_17_MISPRIMING_SCORE=11.00, MER4B a consensus
PRIMER_PAIR_17_MISPRIMING_SCORE=20.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_17_END_STABILITY=9.7000
PRIMER_RIGHT_17_END_STABILITY=6.7000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_17=115
PRIMER_PAIR_PENALTY_18=1.7134
PRIMER_LEFT_18_PENALTY=1.593911
PRIMER_RIGHT_18_PENALTY=0.119476
PRIMER_LEFT_18_SEQUENCE=ATCGAGACCTATGAGCAGGAGAA
PRIMER_RIGHT_18_SEQUENCE=CAATGGGAGCTAGAGGGTTTAAT
PRIMER_LEFT_18=430,23
PRIMER_RIGHT_18=595,23
PRIMER_LEFT_18_TM=61.594
PRIMER_RIGHT_18_TM=59.881
PRIMER_LEFT_18_GC_PERCENT=47.826
PRIMER_RIGHT_18_GC_PERCENT=43.478
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=2.00
PRIMER_LEFT_18_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_18_MISPRIMING_SCORE=12.00, LTR9 LTR from human endogenous retrovirus-like sequence (HUERS-P3)
PRIMER_PAIR_18_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_18_END_STABILITY=6.7000
PRIMER_RIGHT_18_END_STABILITY=6.2000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_18=166
PRIMER_PAIR_PENALTY_19=1.7253
PRIMER_LEFT_19_PENALTY=1.605851
PRIMER_RIGHT_19_PENALTY=0.119476
PRIMER_LEFT_19_SEQUENCE=TCTACCAGCGAACGAAATAAGG
PRIMER_RIGHT_19_SEQUENCE=CAATGGGAGCTAGAGGGTTTAAT
PRIMER_LEFT_19=467,22
PRIMER_RIGHT_19=595,23
PRIMER_LEFT_19_TM=60.606
PRIMER_RIGHT_19_TM=59.881
PRIMER_LEFT_19_GC_PERCENT=45.455
PRIMER_RIGHT_19_GC_PERCENT=43.478
PRIMER_LEFT_19_SELF_ANY=2.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=2.00
PRIMER_LEFT_19_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_19_MISPRIMING_SCORE=12.00, LTR9 LTR from human endogenous retrovirus-like sequence (HUERS-P3)
PRIMER_PAIR_19_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_19_END_STABILITY=7.5000
PRIMER_RIGHT_19_END_STABILITY=6.2000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_19=129
=