PRIMER_SEQUENCE_ID=NM_018276
SEQUENCE=ATGGCCCTGGTCACAGTGAGCCGTTCGCCCCCGGGCAGCGGCGCCTCCACGCCCGTGGGGCCCTGGGACCAGGCGGTCCAGCGAAGGAGTCGACTCCAGCGAAGGCAGAGCTTTGCGGTGCTCCGTGGGGCTGTCCTGGGACTGCAGGATGGAGGGGACAATGATGATGCAGTAGAGGCCAGTTCTGAGCCAACAGAGAAGGCCCCGAGTGAGGAGGAGCTCCACGGGGACCAGACAGACTTCGGGCAAGGATCCCAGAGTCCCCAGAAGCAGGAGGAGCAGAGGCAGCACCTGCACCTCATGGTACAGCTGCTGAGGCCGCAGGATGACATCCGCCTGGCAGCCCAGCTGGAGGCACCCCGGCCTCCCCGGCTCCGCTACCTGCTGGTAGTTTCTACACGAGAAGGAGAAGGTCTGAGCCAGGATGAGACGGTCCTCCTGGGCGTGGATTTCCCTGACAGCAGCTCCCCCAGCTGCACCCTGGGCCTGGTCTTGCCCCTCTGGAGTGACACCCAGGTGTACTTAGATGGAGACGGGGGCTTCAGCGTGACGTCTGGTGGGCAAAGCCGGATCTTCAAGCCCATCTCCATCCAGACCATGTGGGCCACACTCCAGGTATTGCACCAAGCATGTGAGGCAGCTCTAGGCAGCGGCCTTGTACCGGGTGGCAGTGCCCTCACCTGGGCCAGCCACTACCAGGAGAGACTGAACTCCGAACAGAGCTGCCTCAATGAGTGGACGGCTATGGCCGACCTGGAGTCTCTGCGGCCTCCCAGCGCCGAGCCTGGCGGGTCCTCAGAACAGGAGCAGATGGAGCAGGCGATCCGTGCTGAGCTGTGGAAAGTGTTGGATGTCAGTGACCTGGAGAGTGTCACTTCCAAAGAGATCCGCCAGGCTCTGGAGCTGCGCCTGGGGCTCCCCCTCCAGCAGTACCGTGACTTCATCGACAACCAGATGCTGCTGCTGGTGGCACAGCGGGACCGAGCCTCCCGCATCTTCCCCCACCTCTACCTGGGCTCAGAGTGGAACGCAGCAAACCTGGAGGAGCTGCAGAGGAACAGGGTCACCCACATCTTGAACATGGCCCGGGAGATTGACAACTTCTACCCTGAGCGCTTCACCTACCACAATGTGCGCCTCTGGGATGAGGAGTCGGCCCAGCTGCTGCCGCACTGGAAGGAGACGCACCGCTTCATTGAGGCTGCAAGAGCACAGGGCACCCACGTGCTGGTCCACTGCAAGATGGGCGTCAGCCGCTCAGCGGCCACAGTGCTGGCCTATGCCATGAAGCAGTACGAATGCAGCCTGGAGCAGGCCCTGCGCCACGTGCAGGAGCTCCGGCCCATCGCCCGCCCCAACCCTGGCTTCCTGCGCCAGCTGCAGATCTACCAGGGCATCCTGACGGCCAGAACCTGAGGGTGGTGGGGAGGAGAAGGTTGTAGGCATGGAAGAGAGCCAGGCAGCCCCGAAAGAAGAGCCTGGGCCACGGCCACGTATAAACCTCCGAGGGGTCATGAGGTCCATCAGTCTTCTGGAGCCCTCCTTGGAGCTGGAGAGCACCTCAGAGACCAGTGACATGCCAGAGGTCTTCTCTTCCCACGAGTCTTCACATGAAGAGCCTCTGCAGCCCTTCCCACAGCTTGCAAGGACCAAGGGAGGCCAGCAGGTGGACAGGGGGCCTCAGCCTGCCCTGAAGTCCCGCCAGTCAGTGGTTACCCTCCAGGGCAGTGCCGTGGTGGCCAACCGGACCCAGGCCTTCCAGGAGCAGGAGCAGGGGCAGGGGCAGGGGCAGGGAGAGCCCTGCATTTCCTCTACGCCCAGGTTCCGGAAGGTGGTGAGACAGGCCAGCGTGCATGACAGTGGAGAGGAGGGCGAGGCCTGAGCCCTCACACATGCCCACGCTCCCCTGACACTGAAGAGGATCCACAACTCCTTGGAGAAACACCCTCACGTCTGTTGCCGCACACATTCCTCTCAGCTCCGCCCCATACCCGTCACTACAGCCTCACCTCCCACCCCTGTCACTACGGCCTCACCTCCCACCCCTGTCACTACAGCCTCACCTCCTACAGCCTTAAGTCCCAGGCCCATGTCTGCCTGTCCAAGGGCTCAAGACTTTCTAACTGGGATGTGGTAGAGGGACTGAAGGTACCTTTGGGGGCAACAGCACCCTAGTTTCATTCTCAACTCTAGCCCTGCACACTCACCTGTGGCACGGAATGAAAACAGAGCTTCCCGTGCAAAAAGGGTCACGCCTCCCACCCCCGCCCCCTCCCTGCACCTCCTGTCCTCTCCCAGTTCATTCCTGGAACCAGCCAGGCCAGGCAACCAGTGGCCCCCAAAGGCAGGCAGGATCCTCAGGCCCCAGCCGCGGGAGGCTGGAAGGGCTGGCAGATCGCTTCCCTCATCCACCTCCACCGGTCCAGGTCTTTGCTGCTGTCCCCAGACCTCCTGTGACACCACGCCAGATCACAGGGCACCAGGCCAGAGATAGTCTTCTTTTTGTCCTTTCTGGCCTCTGGCTAGTCAGTTTTTCATAGCCTTACAGTATCTGGCTTTGTACTGAGAAATAAAACACATTTTCATAAAAAAAAAAAAAAAAAA
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 30060, GC content failed 1255, low tm 6037, high tm 17329, high any compl 12, high end compl 20, high repeat similarity 61, ok 5346
PRIMER_RIGHT_EXPLAIN=considered 30060, GC content failed 1046, low tm 6530, high tm 16978, high any compl 3, high end compl 10, high repeat similarity 9, ok 5484
PRIMER_PAIR_EXPLAIN=considered 739, unacceptable product size 680, high end compl 3, ok 56
PRIMER_PAIR_PENALTY=0.1088
PRIMER_LEFT_PENALTY=0.061028
PRIMER_RIGHT_PENALTY=0.047725
PRIMER_LEFT_SEQUENCE=CAGGTGTACTTAGATGGAGACGG
PRIMER_RIGHT_SEQUENCE=AGGTCACTGACATCCAACACTTT
PRIMER_LEFT=513,23
PRIMER_RIGHT=862,23
PRIMER_LEFT_TM=60.061
PRIMER_RIGHT_TM=59.952
PRIMER_LEFT_GC_PERCENT=52.174
PRIMER_RIGHT_GC_PERCENT=43.478
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_MISPRIMING_SCORE=12.00, THE1BR THE-1B Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_RIGHT_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_END_STABILITY=9.6000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=350
PRIMER_PAIR_PENALTY_1=0.2451
PRIMER_LEFT_1_PENALTY=0.197354
PRIMER_RIGHT_1_PENALTY=0.047725
PRIMER_LEFT_1_SEQUENCE=GACACCCAGGTGTACTTAGATGG
PRIMER_RIGHT_1_SEQUENCE=AGGTCACTGACATCCAACACTTT
PRIMER_LEFT_1=507,23
PRIMER_RIGHT_1=862,23
PRIMER_LEFT_1_TM=59.803
PRIMER_RIGHT_1_TM=59.952
PRIMER_LEFT_1_GC_PERCENT=52.174
PRIMER_RIGHT_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_MISPRIMING_SCORE=12.00, LTR9 LTR from human endogenous retrovirus-like sequence (HUERS-P3)
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_1_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=356
PRIMER_PAIR_PENALTY_2=0.2799
PRIMER_LEFT_2_PENALTY=0.232197
PRIMER_RIGHT_2_PENALTY=0.047725
PRIMER_LEFT_2_SEQUENCE=ACCTGCTGGTAGTTTCTACACGA
PRIMER_RIGHT_2_SEQUENCE=AGGTCACTGACATCCAACACTTT
PRIMER_LEFT_2=379,23
PRIMER_RIGHT_2=862,23
PRIMER_LEFT_2_TM=60.232
PRIMER_RIGHT_2_TM=59.952
PRIMER_LEFT_2_GC_PERCENT=47.826
PRIMER_RIGHT_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_2_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_2_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=484
PRIMER_PAIR_PENALTY_3=0.2932
PRIMER_LEFT_3_PENALTY=0.232197
PRIMER_RIGHT_3_PENALTY=0.061028
PRIMER_LEFT_3_SEQUENCE=ACCTGCTGGTAGTTTCTACACGA
PRIMER_RIGHT_3_SEQUENCE=CCGTCTCCATCTAAGTACACCTG
PRIMER_LEFT_3=379,23
PRIMER_RIGHT_3=535,23
PRIMER_LEFT_3_TM=60.232
PRIMER_RIGHT_3_TM=60.061
PRIMER_LEFT_3_GC_PERCENT=47.826
PRIMER_RIGHT_3_GC_PERCENT=52.174
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, THE1BR THE-1B Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_PAIR_3_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=157
PRIMER_PAIR_PENALTY_4=0.2998
PRIMER_LEFT_4_PENALTY=0.100425
PRIMER_RIGHT_4_PENALTY=0.199411
PRIMER_LEFT_4_SEQUENCE=TTTCATTCTCAACTCTAGCCCTG
PRIMER_RIGHT_4_SEQUENCE=AAGAAGACTATCTCTGGCCTGGT
PRIMER_LEFT_4=2175,23
PRIMER_RIGHT_4=2500,23
PRIMER_LEFT_4_TM=59.900
PRIMER_RIGHT_4_TM=59.801
PRIMER_LEFT_4_GC_PERCENT=43.478
PRIMER_RIGHT_4_GC_PERCENT=47.826
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_4_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_4_MISPRIMING_SCORE=20.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=326
PRIMER_PAIR_PENALTY_5=0.3610
PRIMER_LEFT_5_PENALTY=0.100425
PRIMER_RIGHT_5_PENALTY=0.260543
PRIMER_LEFT_5_SEQUENCE=TTTCATTCTCAACTCTAGCCCTG
PRIMER_RIGHT_5_SEQUENCE=AAAGAAGACTATCTCTGGCCTGG
PRIMER_LEFT_5=2175,23
PRIMER_RIGHT_5=2501,23
PRIMER_LEFT_5_TM=59.900
PRIMER_RIGHT_5_TM=60.261
PRIMER_LEFT_5_GC_PERCENT=43.478
PRIMER_RIGHT_5_GC_PERCENT=47.826
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=5.00
PRIMER_LEFT_5_SELF_END=2.00
PRIMER_RIGHT_5_SELF_END=3.00
PRIMER_LEFT_5_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_5_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_5_MISPRIMING_SCORE=20.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_5_END_STABILITY=9.7000
PRIMER_RIGHT_5_END_STABILITY=9.7000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_5=327
PRIMER_PAIR_PENALTY_6=0.4010
PRIMER_LEFT_6_PENALTY=0.353311
PRIMER_RIGHT_6_PENALTY=0.047725
PRIMER_LEFT_6_SEQUENCE=CCTGCTGGTAGTTTCTACACGAG
PRIMER_RIGHT_6_SEQUENCE=AGGTCACTGACATCCAACACTTT
PRIMER_LEFT_6=380,23
PRIMER_RIGHT_6=862,23
PRIMER_LEFT_6_TM=60.353
PRIMER_RIGHT_6_TM=59.952
PRIMER_LEFT_6_GC_PERCENT=52.174
PRIMER_RIGHT_6_GC_PERCENT=43.478
PRIMER_LEFT_6_SELF_ANY=5.00
PRIMER_RIGHT_6_SELF_ANY=7.00
PRIMER_LEFT_6_SELF_END=3.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_6_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_6_MISPRIMING_SCORE=24.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_6_END_STABILITY=8.1000
PRIMER_RIGHT_6_END_STABILITY=6.7000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_6=483
PRIMER_PAIR_PENALTY_7=0.4143
PRIMER_LEFT_7_PENALTY=0.353311
PRIMER_RIGHT_7_PENALTY=0.061028
PRIMER_LEFT_7_SEQUENCE=CCTGCTGGTAGTTTCTACACGAG
PRIMER_RIGHT_7_SEQUENCE=CCGTCTCCATCTAAGTACACCTG
PRIMER_LEFT_7=380,23
PRIMER_RIGHT_7=535,23
PRIMER_LEFT_7_TM=60.353
PRIMER_RIGHT_7_TM=60.061
PRIMER_LEFT_7_GC_PERCENT=52.174
PRIMER_RIGHT_7_GC_PERCENT=52.174
PRIMER_LEFT_7_SELF_ANY=5.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=3.00
PRIMER_RIGHT_7_SELF_END=2.00
PRIMER_LEFT_7_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_7_MISPRIMING_SCORE=12.00, THE1BR THE-1B Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_PAIR_7_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_7_END_STABILITY=8.1000
PRIMER_RIGHT_7_END_STABILITY=7.9000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_7=156
PRIMER_PAIR_PENALTY_8=0.4914
PRIMER_LEFT_8_PENALTY=0.291983
PRIMER_RIGHT_8_PENALTY=0.199411
PRIMER_LEFT_8_SEQUENCE=AGGGCTCAAGACTTTCTAACTGG
PRIMER_RIGHT_8_SEQUENCE=AAGAAGACTATCTCTGGCCTGGT
PRIMER_LEFT_8=2104,23
PRIMER_RIGHT_8=2500,23
PRIMER_LEFT_8_TM=60.292
PRIMER_RIGHT_8_TM=59.801
PRIMER_LEFT_8_GC_PERCENT=47.826
PRIMER_RIGHT_8_GC_PERCENT=47.826
PRIMER_LEFT_8_SELF_ANY=5.00
PRIMER_RIGHT_8_SELF_ANY=5.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_8_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_8_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_8_END_STABILITY=7.9000
PRIMER_RIGHT_8_END_STABILITY=7.9000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_8=397
PRIMER_PAIR_PENALTY_9=0.5208
PRIMER_LEFT_9_PENALTY=0.197354
PRIMER_RIGHT_9_PENALTY=0.323473
PRIMER_LEFT_9_SEQUENCE=GACACCCAGGTGTACTTAGATGG
PRIMER_RIGHT_9_SEQUENCE=CTCTGTTCGGAGTTCAGTCTCTC
PRIMER_LEFT_9=507,23
PRIMER_RIGHT_9=721,23
PRIMER_LEFT_9_TM=59.803
PRIMER_RIGHT_9_TM=59.677
PRIMER_LEFT_9_GC_PERCENT=52.174
PRIMER_RIGHT_9_GC_PERCENT=52.174
PRIMER_LEFT_9_SELF_ANY=8.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=2.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_MISPRIMING_SCORE=12.00, LTR9 LTR from human endogenous retrovirus-like sequence (HUERS-P3)
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_9_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_9_END_STABILITY=8.1000
PRIMER_RIGHT_9_END_STABILITY=6.4000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_9=215
PRIMER_PAIR_PENALTY_10=0.5525
PRIMER_LEFT_10_PENALTY=0.291983
PRIMER_RIGHT_10_PENALTY=0.260543
PRIMER_LEFT_10_SEQUENCE=AGGGCTCAAGACTTTCTAACTGG
PRIMER_RIGHT_10_SEQUENCE=AAAGAAGACTATCTCTGGCCTGG
PRIMER_LEFT_10=2104,23
PRIMER_RIGHT_10=2501,23
PRIMER_LEFT_10_TM=60.292
PRIMER_RIGHT_10_TM=60.261
PRIMER_LEFT_10_GC_PERCENT=47.826
PRIMER_RIGHT_10_GC_PERCENT=47.826
PRIMER_LEFT_10_SELF_ANY=5.00
PRIMER_RIGHT_10_SELF_ANY=5.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=3.00
PRIMER_LEFT_10_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_10_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_10_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_10_END_STABILITY=7.9000
PRIMER_RIGHT_10_END_STABILITY=9.7000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_10=398
PRIMER_PAIR_PENALTY_11=0.5557
PRIMER_LEFT_11_PENALTY=0.232197
PRIMER_RIGHT_11_PENALTY=0.323473
PRIMER_LEFT_11_SEQUENCE=ACCTGCTGGTAGTTTCTACACGA
PRIMER_RIGHT_11_SEQUENCE=CTCTGTTCGGAGTTCAGTCTCTC
PRIMER_LEFT_11=379,23
PRIMER_RIGHT_11=721,23
PRIMER_LEFT_11_TM=60.232
PRIMER_RIGHT_11_TM=59.677
PRIMER_LEFT_11_GC_PERCENT=47.826
PRIMER_RIGHT_11_GC_PERCENT=52.174
PRIMER_LEFT_11_SELF_ANY=6.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=2.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_11_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_11_END_STABILITY=8.4000
PRIMER_RIGHT_11_END_STABILITY=6.4000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_11=343
PRIMER_PAIR_PENALTY_12=0.5831
PRIMER_LEFT_12_PENALTY=0.061028
PRIMER_RIGHT_12_PENALTY=0.522051
PRIMER_LEFT_12_SEQUENCE=CAGGTGTACTTAGATGGAGACGG
PRIMER_RIGHT_12_SEQUENCE=ATTCGTACTGCTTCATGGCATAG
PRIMER_LEFT_12=513,23
PRIMER_RIGHT_12=1299,23
PRIMER_LEFT_12_TM=60.061
PRIMER_RIGHT_12_TM=60.522
PRIMER_LEFT_12_GC_PERCENT=52.174
PRIMER_RIGHT_12_GC_PERCENT=43.478
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=5.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=3.00
PRIMER_LEFT_12_MISPRIMING_SCORE=12.00, THE1BR THE-1B Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_RIGHT_12_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_12_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_12_END_STABILITY=9.6000
PRIMER_RIGHT_12_END_STABILITY=5.9000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_12=787
PRIMER_PAIR_PENALTY_13=0.6225
PRIMER_LEFT_13_PENALTY=0.522051
PRIMER_RIGHT_13_PENALTY=0.100425
PRIMER_LEFT_13_SEQUENCE=CTATGCCATGAAGCAGTACGAAT
PRIMER_RIGHT_13_SEQUENCE=CAGGGCTAGAGTTGAGAATGAAA
PRIMER_LEFT_13=1277,23
PRIMER_RIGHT_13=2197,23
PRIMER_LEFT_13_TM=60.522
PRIMER_RIGHT_13_TM=59.900
PRIMER_LEFT_13_GC_PERCENT=43.478
PRIMER_RIGHT_13_GC_PERCENT=43.478
PRIMER_LEFT_13_SELF_ANY=5.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=2.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_13_MISPRIMING_SCORE=12.00, reverse L1MC2 3'-end of L1 repeat (subfamily L1MC2) - a consensus sequence
PRIMER_PAIR_13_MISPRIMING_SCORE=21.00, reverse MER4C a consensus
PRIMER_LEFT_13_END_STABILITY=8.6000
PRIMER_RIGHT_13_END_STABILITY=7.3000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_13=921
PRIMER_PAIR_PENALTY_14=0.6345
PRIMER_LEFT_14_PENALTY=0.112479
PRIMER_RIGHT_14_PENALTY=0.522051
PRIMER_LEFT_14_SEQUENCE=GGGAGATTGACAACTTCTACCCT
PRIMER_RIGHT_14_SEQUENCE=ATTCGTACTGCTTCATGGCATAG
PRIMER_LEFT_14=1087,23
PRIMER_RIGHT_14=1299,23
PRIMER_LEFT_14_TM=59.888
PRIMER_RIGHT_14_TM=60.522
PRIMER_LEFT_14_GC_PERCENT=47.826
PRIMER_RIGHT_14_GC_PERCENT=43.478
PRIMER_LEFT_14_SELF_ANY=5.00
PRIMER_RIGHT_14_SELF_ANY=5.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=3.00
PRIMER_LEFT_14_MISPRIMING_SCORE=11.00, reverse MLT2B2 Interspersed repeat MLT2B2 - a consensus
PRIMER_RIGHT_14_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_14_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_14_END_STABILITY=9.1000
PRIMER_RIGHT_14_END_STABILITY=5.9000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_14=213
PRIMER_PAIR_PENALTY_15=0.6696
PRIMER_LEFT_15_PENALTY=0.470165
PRIMER_RIGHT_15_PENALTY=0.199411
PRIMER_LEFT_15_SEQUENCE=TTCTAACTGGGATGTGGTAGAGG
PRIMER_RIGHT_15_SEQUENCE=AAGAAGACTATCTCTGGCCTGGT
PRIMER_LEFT_15=2117,23
PRIMER_RIGHT_15=2500,23
PRIMER_LEFT_15_TM=59.530
PRIMER_RIGHT_15_TM=59.801
PRIMER_LEFT_15_GC_PERCENT=47.826
PRIMER_RIGHT_15_GC_PERCENT=47.826
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=5.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_MISPRIMING_SCORE=11.00, L1MC2 3'-end of L1 repeat (subfamily L1MC2) - a consensus sequence
PRIMER_RIGHT_15_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_15_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_15_END_STABILITY=7.9000
PRIMER_RIGHT_15_END_STABILITY=7.9000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_15=384
PRIMER_PAIR_PENALTY_16=0.6768
PRIMER_LEFT_16_PENALTY=0.353311
PRIMER_RIGHT_16_PENALTY=0.323473
PRIMER_LEFT_16_SEQUENCE=CCTGCTGGTAGTTTCTACACGAG
PRIMER_RIGHT_16_SEQUENCE=CTCTGTTCGGAGTTCAGTCTCTC
PRIMER_LEFT_16=380,23
PRIMER_RIGHT_16=721,23
PRIMER_LEFT_16_TM=60.353
PRIMER_RIGHT_16_TM=59.677
PRIMER_LEFT_16_GC_PERCENT=52.174
PRIMER_RIGHT_16_GC_PERCENT=52.174
PRIMER_LEFT_16_SELF_ANY=5.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=3.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_16_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_16_MISPRIMING_SCORE=24.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_16_END_STABILITY=8.1000
PRIMER_RIGHT_16_END_STABILITY=6.4000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_16=342
PRIMER_PAIR_PENALTY_17=0.7194
PRIMER_LEFT_17_PENALTY=0.197354
PRIMER_RIGHT_17_PENALTY=0.522051
PRIMER_LEFT_17_SEQUENCE=GACACCCAGGTGTACTTAGATGG
PRIMER_RIGHT_17_SEQUENCE=ATTCGTACTGCTTCATGGCATAG
PRIMER_LEFT_17=507,23
PRIMER_RIGHT_17=1299,23
PRIMER_LEFT_17_TM=59.803
PRIMER_RIGHT_17_TM=60.522
PRIMER_LEFT_17_GC_PERCENT=52.174
PRIMER_RIGHT_17_GC_PERCENT=43.478
PRIMER_LEFT_17_SELF_ANY=8.00
PRIMER_RIGHT_17_SELF_ANY=5.00
PRIMER_LEFT_17_SELF_END=2.00
PRIMER_RIGHT_17_SELF_END=3.00
PRIMER_LEFT_17_MISPRIMING_SCORE=12.00, LTR9 LTR from human endogenous retrovirus-like sequence (HUERS-P3)
PRIMER_RIGHT_17_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_17_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_17_END_STABILITY=8.1000
PRIMER_RIGHT_17_END_STABILITY=5.9000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_17=793
PRIMER_PAIR_PENALTY_18=0.7307
PRIMER_LEFT_18_PENALTY=0.470165
PRIMER_RIGHT_18_PENALTY=0.260543
PRIMER_LEFT_18_SEQUENCE=TTCTAACTGGGATGTGGTAGAGG
PRIMER_RIGHT_18_SEQUENCE=AAAGAAGACTATCTCTGGCCTGG
PRIMER_LEFT_18=2117,23
PRIMER_RIGHT_18=2501,23
PRIMER_LEFT_18_TM=59.530
PRIMER_RIGHT_18_TM=60.261
PRIMER_LEFT_18_GC_PERCENT=47.826
PRIMER_RIGHT_18_GC_PERCENT=47.826
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=5.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=3.00
PRIMER_LEFT_18_MISPRIMING_SCORE=11.00, L1MC2 3'-end of L1 repeat (subfamily L1MC2) - a consensus sequence
PRIMER_RIGHT_18_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_18_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_18_END_STABILITY=7.9000
PRIMER_RIGHT_18_END_STABILITY=9.7000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_18=385
PRIMER_PAIR_PENALTY_19=0.7542
PRIMER_LEFT_19_PENALTY=0.232197
PRIMER_RIGHT_19_PENALTY=0.522051
PRIMER_LEFT_19_SEQUENCE=ACCTGCTGGTAGTTTCTACACGA
PRIMER_RIGHT_19_SEQUENCE=ATTCGTACTGCTTCATGGCATAG
PRIMER_LEFT_19=379,23
PRIMER_RIGHT_19=1299,23
PRIMER_LEFT_19_TM=60.232
PRIMER_RIGHT_19_TM=60.522
PRIMER_LEFT_19_GC_PERCENT=47.826
PRIMER_RIGHT_19_GC_PERCENT=43.478
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=5.00
PRIMER_LEFT_19_SELF_END=2.00
PRIMER_RIGHT_19_SELF_END=3.00
PRIMER_LEFT_19_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_19_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_19_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_19_END_STABILITY=8.4000
PRIMER_RIGHT_19_END_STABILITY=5.9000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_19=921
=