PRIMER_SEQUENCE_ID=NM_018984
SEQUENCE=CCGGGCCCGGCCCGGGCGGCGGTGGCGGTGGCGGCTCTAGCTCGAGACGTCTGTGGCGCCCTCGCACCGCGCCGCAGCCATGGTCCTGGTGACCCTGCAGCGCTCGCCCACGCCCAGCGCCGCCTCCTCCTCGGCCAGCAACAGCGAGTTGGAGGCTGGCAGCGAAGAAGATCGAAAATTAAACCTCAGCTTAAGTGAGAGCTTTTTCATGGTGAAAGGCGCAGCCCTCTTCTTACAACAGGGAAGCAGCCCTCAAGGCCAGCGGAGTCTTCAGCACCCCCACAAGCATGCAGGTGATCTGCCTCAACATCTTCAGGTGATGATCAACCTTCTGCGTTGCGAAGACAGAATCAAGCTGGCAGTGCGCCTGGAGAGCGCCTGGGCGGACCGGGTCCGGTACATGGTGGTGGTGTACAGCAGCGGGCGCCAGGACACCGAGGAGAATATCTTGCTGGGAGTGGACTTTTCCAGTAAGGAAAGTAAAAGCTGCACCATTGGGATGGTTCTCCGACTGTGGAGCGACACGAAAATCCACCTTGATGGAGATGGTGGGTTCAGCGTGAGCACAGCAGGAAGGATGCACATATTTAAGCCTGTGTCTGTCCAGGCCATGTGGTCTGCCCTGCAGGTGCTTCACAAGGCCTGCGAAGTGGCCCGGAGGCACAACTACTTCCCCGGGGGTGTAGCTCTCATCTGGGCTACCTACTATGAGAGCTGCATCAGCTCCGAGCAGAGCTGCATCAACGAGTGGAACGCCATGCAGGACCTGGAGTCTACGCGGCCCGACTCCCCCGCGCTATTTGTGGACAAGCCCACTGAAGGGGAAAGGACCGAGCGCCTCATCAAAGCCAAGCTCCGAAGCATCATGATGAGCCAGGATCTAGAAAATGTGACTTCCAAAGAGATTCGTAATGAATTAGAGAAACAGATGAATTGTAACTTGAAGGAACTCAAGGAATTTATAGACAATGAGATGCTACTTATCTTGGGACAGATGGACAAGCCCTCCCTTATCTTCGATCATCTTTATCTCGGCTCTGAATGGAATGCATCCAATCTGGAGGAACTGCAGGGCTCAGGGGTTGATTACATTTTAAATGTTACCAGAGAAATCGATAATTTTTTTCCTGGCTTATTTGCATATCATAACATCCGAGTCTACGATGAAGAGACCACAGACCTCCTCGCCCACTGGAATGAAGCGTATCATTTTATAAACAAAGCGAAGAGGAACCATTCCAAGTGCCTGGTGCATTGCAAAATGGGCGTGAGTCGCTCGGCCTCCACAGTCATAGCCTATGCAATGAAGGAATTCGGCTGGCCTCTGGAAAAAGCATATAACTATGTAAAGCAGAAGCGCAGCATCACGCGCCCCAACGCGGGCTTTATGAGGCAGCTGTCTGAGTATGAAGGCATCTTGGATGCAAGCAAACAGCGGCACAACAAGCTGTGGCGTCAGCAGACAGACAGCAGCCTCCAGCAGCCTGTGGATGACCCTGCAGGACCTGGCGACTTCTTGCCAGAGACCCCAGATGGCACCCCGGAAAGCCAGCTGCCCTTCTTGGATGATGCCGCCCAGCCCGGCTTAGGGCCCCCCCTCCCCTGCTGTTTCCGGCGACTCTCAGACCCCCTTCTGCCTTCCCCTGAGGATGAAACTGGCAGCTTGGTCCACCTGGAGGATCCGGAGAGGGAGGCTCTGTTGGAGGAAGCTGCTCCACCTGCAGAGGTGCACAGGCCGGCCAGACAGCCCCAGCAAGGTTCCGGACTCTGTGAGAAGGATGTGAAGAAGAAACTAGAGTTTGGGAGTCCCAAAGGTCGGAGCGGCTCCTTGCTGCAGGTGGAGGAGACGGAAAGGGAGGAGGGCCTGGGAGCAGGGAGGTGGGGGCAGCTTCCAACCCAGCTCGATCAAAACCTGCTCAACTCGGAGAACCTAAACAACAACAGCAAGAGGAGCTGTCCCAACGGCATGGAGGATGATGCTATATTTGGGATCCTTAACAAAGTGAAGCCTTCCTATAAATCCTGTGCCGACTGCATGTACCCTACAGCCAGCGGGGCTCCTGAGGCCTCCAGGGAGCGATGTGAGGACCCCAATGCTCCCGCCATCTGCACCCAGCCAGCCTTCCTACCCCACATCACGTCCTCCCCTGTGGCCCACTTGGCCAGCAGGTCCCGTGTTCCGGAGAAGCCAGCCTCTGGCCCAACCGAACCTCCCCCGTTCCTACCACCAGCAGGCTCCAGGAGGGCAGACACCAGTGGCCCTGGGGCTGGAGCTGCCCTAGAGCCACCAGCCAGCCTTTTGGAACCTTCCAGAGAGACCCCAAAAGTCCTGCCAAAGTCCCTCCTTTTGAAGAATTCTCACTGTGATAAGAACCCTCCCAGCACAGAAGTGGTAATAAAGGAAGAATCGTCACCCAAGAAAGATATGAAGCCAGCCAAGGACCTGAGGCTTCTGTTCAGTAATGAATCTGAGAAGCCGACAACCAACAGCTACCTGATGCAGCACCAGGAGTCCATCATTCAGCTGCAGAAGGCAGGCTTGGTCCGCAAGCACACCAAAGAGCTAGAGCGGCTGAAGAGCGTGCCTGCAGACCCAGCACCTCCCTCCAGGGATGGCCCTGCCAGCAGGCTGGAGGCCAGCATCCCCGAGGAGAGCCAGGATCCAGCCGCGCTCCACGAGCTGGGCCCCCTGGTTATGCCCAGCCAGGCCGGGAGTGATGAGAAGTCAGAGGCCGCCCCCGCTTCATTGGAAGGAGGCTCACTGAAGAGCCCCCCTCCTTTCTTCTACCGCCTGGACCACACCAGTAGTTTCTCAAAAGACTTTCTGAAGACCATCTGCTACACCCCCACCTCCTCTTCCATGAGCTCCAACCTGACCCGGAGTTCCAGCAGCGATAGCATCCACAGTGTCCGTGGGAAGCCCGGGCTGGTGAAGCAGCGGACGCAGGAGATTGAGACCCGGCTCCGGCTGGCGGGCCTCACCGTCTCTTCCCCACTGAAGCGCTCACACTCTCTTGCCAAGCTGGGGAGTCTCACCTTCTCAACGGAAGACCTGTCCAGTGAGGCTGACCCGTCCACCGTCGCTGACTCCCAGGACACCACTTTGAGTGAATCTTCCTTCTTGCATGAGCCCCAGGGAACCCCGAGGGACCCAGCTGCAACCTCCAAACCATCAGGGAAACCCGCCCCAGAAAACTTAAAGAGCCCTTCGTGGATGAGCAAAAGCTGACCCGCCTTTTGCTGCGTCAGGCTGGGCGGATATTTTCATATGATCCCTCCCTCACTTTCACTGTGGATTTGGATCGACCCCTTACATCTTGATTTCCCTTAAACGAGTGAAGTCATCCTAACTTTTCCCCTACTCTTGCCATAGAGAGGAGGAGAAGAAACTAACCAACATGCAGCACAAGAAGGGGAGCTGTCAGTCGTGTGGCCTGGGAGAACCAGGAGCCGCCGGCCAGGAGACACAAAACTCCGCTCCCACCGTGTCTTCAAGAAGAACCATGTTTTAGGAAGAACGTGCACACATAGGCGCACACATCCAGACTGTTCCCTTCGCATCCTGCAGAAGAGTCCTGGTGGTGGCAGCCTCACCGCGGCACACGTTCTAGCTCATTCTTGGCCTCGCAGAAAACTCTCGGATGGCAACATTAAGTCCTACCTCTGTTCTCGCTGCGCTTTTTATTTTTAAAACACACGAATGACCAAGGCTTGTTCCAGGGAATAATGCTCTGTCTGAAAGCGTTTTTCCAAAAAAAGAATTTTTCGTTTTGTGTTTTGGTTGGGACCCCAGACGTGTCAAGACTTTTAACCCGACAGCCCCAAGCACTGCTGAGTAAAGTCATCAGATGAGTAGTCCCACGCTGTTGTTGAGCTGACTCTGTGCCCGAATGGTGGTGCAGGCGCCCTGTCCTGGTTGTGTTGTTACGTGTTTGACCAGCACAGGGGTCCGGTGGGGAAAATGTATGGGTTGTTCTCAGTTGTTGCTAATGCTGAAGTTTAAATCTCAAGGGGAAGGGCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 47183, GC content failed 1226, low tm 13885, high tm 22519, high any compl 11, high end compl 54, high repeat similarity 75, long poly-x seq 86, ok 9327
PRIMER_RIGHT_EXPLAIN=considered 47061, GC content failed 1427, low tm 13954, high tm 22131, high any compl 2, high end compl 12, high repeat similarity 18, long poly-x seq 101, ok 9416
PRIMER_PAIR_EXPLAIN=considered 1946, unacceptable product size 1878, high end compl 3, ok 65
PRIMER_PAIR_PENALTY=0.0892
PRIMER_LEFT_PENALTY=0.007752
PRIMER_RIGHT_PENALTY=0.081398
PRIMER_LEFT_SEQUENCE=CGTCACCCAAGAAAGATATGAAG
PRIMER_RIGHT_SEQUENCE=GAGAAACTACTGGTGTGGTCCAG
PRIMER_LEFT=2408,23
PRIMER_RIGHT=2813,23
PRIMER_LEFT_TM=60.008
PRIMER_RIGHT_TM=60.081
PRIMER_LEFT_GC_PERCENT=43.478
PRIMER_RIGHT_GC_PERCENT=52.174
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_MISPRIMING_SCORE=12.00, L1PA2 3'-end of L1 repeat (subfamily L1PA2) - a consensus sequence
PRIMER_RIGHT_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=406
PRIMER_PAIR_PENALTY_1=0.0896
PRIMER_LEFT_1_PENALTY=0.008181
PRIMER_RIGHT_1_PENALTY=0.081398
PRIMER_LEFT_1_SEQUENCE=CAAAGTCCCTCCTTTTGAAGAAT
PRIMER_RIGHT_1_SEQUENCE=GAGAAACTACTGGTGTGGTCCAG
PRIMER_LEFT_1=2333,23
PRIMER_RIGHT_1=2813,23
PRIMER_LEFT_1_TM=59.992
PRIMER_RIGHT_1_TM=60.081
PRIMER_LEFT_1_GC_PERCENT=39.130
PRIMER_RIGHT_1_GC_PERCENT=52.174
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_1_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_1_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=481
PRIMER_PAIR_PENALTY_2=0.0965
PRIMER_LEFT_2_PENALTY=0.007752
PRIMER_RIGHT_2_PENALTY=0.088773
PRIMER_LEFT_2_SEQUENCE=CGTCACCCAAGAAAGATATGAAG
PRIMER_RIGHT_2_SEQUENCE=AAATCAAGATGTAAGGGGTCGAT
PRIMER_LEFT_2=2408,23
PRIMER_RIGHT_2=3323,23
PRIMER_LEFT_2_TM=60.008
PRIMER_RIGHT_2_TM=60.089
PRIMER_LEFT_2_GC_PERCENT=43.478
PRIMER_RIGHT_2_GC_PERCENT=39.130
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, L1PA2 3'-end of L1 repeat (subfamily L1PA2) - a consensus sequence
PRIMER_RIGHT_2_MISPRIMING_SCORE=12.00, MARINER1 Human mariner
PRIMER_PAIR_2_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=8.3000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=916
PRIMER_PAIR_PENALTY_3=0.0970
PRIMER_LEFT_3_PENALTY=0.008181
PRIMER_RIGHT_3_PENALTY=0.088773
PRIMER_LEFT_3_SEQUENCE=CAAAGTCCCTCCTTTTGAAGAAT
PRIMER_RIGHT_3_SEQUENCE=AAATCAAGATGTAAGGGGTCGAT
PRIMER_LEFT_3=2333,23
PRIMER_RIGHT_3=3323,23
PRIMER_LEFT_3_TM=59.992
PRIMER_RIGHT_3_TM=60.089
PRIMER_LEFT_3_GC_PERCENT=39.130
PRIMER_RIGHT_3_GC_PERCENT=39.130
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, MARINER1 Human mariner
PRIMER_PAIR_3_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=8.3000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=991
PRIMER_PAIR_PENALTY_4=0.1229
PRIMER_LEFT_4_PENALTY=0.007752
PRIMER_RIGHT_4_PENALTY=0.115144
PRIMER_LEFT_4_SEQUENCE=CGTCACCCAAGAAAGATATGAAG
PRIMER_RIGHT_4_SEQUENCE=GCAAGAAGGAAGATTCACTCAAA
PRIMER_LEFT_4=2408,23
PRIMER_RIGHT_4=3124,23
PRIMER_LEFT_4_TM=60.008
PRIMER_RIGHT_4_TM=59.885
PRIMER_LEFT_4_GC_PERCENT=43.478
PRIMER_RIGHT_4_GC_PERCENT=39.130
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, L1PA2 3'-end of L1 repeat (subfamily L1PA2) - a consensus sequence
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, reverse MER11A a consensus
PRIMER_PAIR_4_MISPRIMING_SCORE=21.00, MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=717
PRIMER_PAIR_PENALTY_5=0.1525
PRIMER_LEFT_5_PENALTY=0.144318
PRIMER_RIGHT_5_PENALTY=0.008181
PRIMER_LEFT_5_SEQUENCE=GTCTGAGTATGAAGGCATCTTGG
PRIMER_RIGHT_5_SEQUENCE=ATTCTTCAAAAGGAGGGACTTTG
PRIMER_LEFT_5=1398,23
PRIMER_RIGHT_5=2355,23
PRIMER_LEFT_5_TM=60.144
PRIMER_RIGHT_5_TM=59.992
PRIMER_LEFT_5_GC_PERCENT=47.826
PRIMER_RIGHT_5_GC_PERCENT=39.130
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_5_MISPRIMING_SCORE=11.00, reverse L1MC2 3'-end of L1 repeat (subfamily L1MC2) - a consensus sequence
PRIMER_PAIR_5_MISPRIMING_SCORE=21.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_5_END_STABILITY=8.5000
PRIMER_RIGHT_5_END_STABILITY=7.3000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_5=958
PRIMER_PAIR_PENALTY_6=0.1636
PRIMER_LEFT_6_PENALTY=0.055411
PRIMER_RIGHT_6_PENALTY=0.108177
PRIMER_LEFT_6_SEQUENCE=CCTCCCTTATCTTCGATCATCTT
PRIMER_RIGHT_6_SEQUENCE=CTGCGCTTCTGCTTTACATAGTT
PRIMER_LEFT_6=1004,23
PRIMER_RIGHT_6=1361,23
PRIMER_LEFT_6_TM=59.945
PRIMER_RIGHT_6_TM=60.108
PRIMER_LEFT_6_GC_PERCENT=43.478
PRIMER_RIGHT_6_GC_PERCENT=43.478
PRIMER_LEFT_6_SELF_ANY=5.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_6_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_6_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_6_END_STABILITY=6.6000
PRIMER_RIGHT_6_END_STABILITY=5.7000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_6=358
PRIMER_PAIR_PENALTY_7=0.1642
PRIMER_LEFT_7_PENALTY=0.108818
PRIMER_RIGHT_7_PENALTY=0.055411
PRIMER_LEFT_7_SEQUENCE=GAGGAGAATATCTTGCTGGGAGT
PRIMER_RIGHT_7_SEQUENCE=AAGATGATCGAAGATAAGGGAGG
PRIMER_LEFT_7=436,23
PRIMER_RIGHT_7=1026,23
PRIMER_LEFT_7_TM=60.109
PRIMER_RIGHT_7_TM=59.945
PRIMER_LEFT_7_GC_PERCENT=47.826
PRIMER_RIGHT_7_GC_PERCENT=43.478
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=5.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_MISPRIMING_SCORE=11.00, MER34 a consensus
PRIMER_RIGHT_7_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_7_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_7_END_STABILITY=7.6000
PRIMER_RIGHT_7_END_STABILITY=9.4000
PRIMER_PAIR_7_COMPL_ANY=6.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_7=591
PRIMER_PAIR_PENALTY_8=0.1702
PRIMER_LEFT_8_PENALTY=0.081398
PRIMER_RIGHT_8_PENALTY=0.088773
PRIMER_LEFT_8_SEQUENCE=CTGGACCACACCAGTAGTTTCTC
PRIMER_RIGHT_8_SEQUENCE=AAATCAAGATGTAAGGGGTCGAT
PRIMER_LEFT_8=2791,23
PRIMER_RIGHT_8=3323,23
PRIMER_LEFT_8_TM=60.081
PRIMER_RIGHT_8_TM=60.089
PRIMER_LEFT_8_GC_PERCENT=52.174
PRIMER_RIGHT_8_GC_PERCENT=39.130
PRIMER_LEFT_8_SELF_ANY=5.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=2.00
PRIMER_LEFT_8_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, MARINER1 Human mariner
PRIMER_PAIR_8_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_8_END_STABILITY=6.7000
PRIMER_RIGHT_8_END_STABILITY=8.3000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_8=533
PRIMER_PAIR_PENALTY_9=0.1965
PRIMER_LEFT_9_PENALTY=0.081398
PRIMER_RIGHT_9_PENALTY=0.115144
PRIMER_LEFT_9_SEQUENCE=CTGGACCACACCAGTAGTTTCTC
PRIMER_RIGHT_9_SEQUENCE=GCAAGAAGGAAGATTCACTCAAA
PRIMER_LEFT_9=2791,23
PRIMER_RIGHT_9=3124,23
PRIMER_LEFT_9_TM=60.081
PRIMER_RIGHT_9_TM=59.885
PRIMER_LEFT_9_GC_PERCENT=52.174
PRIMER_RIGHT_9_GC_PERCENT=39.130
PRIMER_LEFT_9_SELF_ANY=5.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, reverse MER11A a consensus
PRIMER_PAIR_9_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_9_END_STABILITY=6.7000
PRIMER_RIGHT_9_END_STABILITY=7.3000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_9=334
PRIMER_PAIR_PENALTY_10=0.2039
PRIMER_LEFT_10_PENALTY=0.115144
PRIMER_RIGHT_10_PENALTY=0.088773
PRIMER_LEFT_10_SEQUENCE=TTTGAGTGAATCTTCCTTCTTGC
PRIMER_RIGHT_10_SEQUENCE=AAATCAAGATGTAAGGGGTCGAT
PRIMER_LEFT_10=3102,23
PRIMER_RIGHT_10=3323,23
PRIMER_LEFT_10_TM=59.885
PRIMER_RIGHT_10_TM=60.089
PRIMER_LEFT_10_GC_PERCENT=39.130
PRIMER_RIGHT_10_GC_PERCENT=39.130
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=2.00
PRIMER_RIGHT_10_SELF_END=2.00
PRIMER_LEFT_10_MISPRIMING_SCORE=12.00, reverse MER11A a consensus
PRIMER_RIGHT_10_MISPRIMING_SCORE=12.00, MARINER1 Human mariner
PRIMER_PAIR_10_MISPRIMING_SCORE=20.00, MARINER1 Human mariner
PRIMER_LEFT_10_END_STABILITY=8.5000
PRIMER_RIGHT_10_END_STABILITY=8.3000
PRIMER_PAIR_10_COMPL_ANY=7.00
PRIMER_PAIR_10_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_10=222
PRIMER_PAIR_PENALTY_11=0.2148
PRIMER_LEFT_11_PENALTY=0.008181
PRIMER_RIGHT_11_PENALTY=0.206636
PRIMER_LEFT_11_SEQUENCE=CAAAGTCCCTCCTTTTGAAGAAT
PRIMER_RIGHT_11_SEQUENCE=GAAGGGCTCTTTAAGTTTTCTGG
PRIMER_LEFT_11=2333,23
PRIMER_RIGHT_11=3209,23
PRIMER_LEFT_11_TM=59.992
PRIMER_RIGHT_11_TM=59.793
PRIMER_LEFT_11_GC_PERCENT=39.130
PRIMER_RIGHT_11_GC_PERCENT=43.478
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=6.00
PRIMER_LEFT_11_SELF_END=2.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, L1MB7 3'-end of L1 repeat (subfamily L1MB7) - a consensus sequence
PRIMER_PAIR_11_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_11_END_STABILITY=6.6000
PRIMER_RIGHT_11_END_STABILITY=8.2000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_11=877
PRIMER_PAIR_PENALTY_12=0.2170
PRIMER_LEFT_12_PENALTY=0.108818
PRIMER_RIGHT_12_PENALTY=0.108177
PRIMER_LEFT_12_SEQUENCE=GAGGAGAATATCTTGCTGGGAGT
PRIMER_RIGHT_12_SEQUENCE=CTGCGCTTCTGCTTTACATAGTT
PRIMER_LEFT_12=436,23
PRIMER_RIGHT_12=1361,23
PRIMER_LEFT_12_TM=60.109
PRIMER_RIGHT_12_TM=60.108
PRIMER_LEFT_12_GC_PERCENT=47.826
PRIMER_RIGHT_12_GC_PERCENT=43.478
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_MISPRIMING_SCORE=11.00, MER34 a consensus
PRIMER_RIGHT_12_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_12_MISPRIMING_SCORE=23.00, MER34 a consensus
PRIMER_LEFT_12_END_STABILITY=7.6000
PRIMER_RIGHT_12_END_STABILITY=5.7000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_12=926
PRIMER_PAIR_PENALTY_13=0.2174
PRIMER_LEFT_13_PENALTY=0.115144
PRIMER_RIGHT_13_PENALTY=0.102207
PRIMER_LEFT_13_SEQUENCE=TTTGAGTGAATCTTCCTTCTTGC
PRIMER_RIGHT_13_SEQUENCE=TGAGAACAACCCATACATTTTCC
PRIMER_LEFT_13=3102,23
PRIMER_RIGHT_13=3978,23
PRIMER_LEFT_13_TM=59.885
PRIMER_RIGHT_13_TM=60.102
PRIMER_LEFT_13_GC_PERCENT=39.130
PRIMER_RIGHT_13_GC_PERCENT=39.130
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=2.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_MISPRIMING_SCORE=12.00, reverse MER11A a consensus
PRIMER_RIGHT_13_MISPRIMING_SCORE=12.00, L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_PAIR_13_MISPRIMING_SCORE=20.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_13_END_STABILITY=8.5000
PRIMER_RIGHT_13_END_STABILITY=8.5000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_13=877
PRIMER_PAIR_PENALTY_14=0.2303
PRIMER_LEFT_14_PENALTY=0.122120
PRIMER_RIGHT_14_PENALTY=0.108177
PRIMER_LEFT_14_SEQUENCE=ACTTATCTTGGGACAGATGGACA
PRIMER_RIGHT_14_SEQUENCE=CTGCGCTTCTGCTTTACATAGTT
PRIMER_LEFT_14=978,23
PRIMER_RIGHT_14=1361,23
PRIMER_LEFT_14_TM=59.878
PRIMER_RIGHT_14_TM=60.108
PRIMER_LEFT_14_GC_PERCENT=43.478
PRIMER_RIGHT_14_GC_PERCENT=43.478
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=2.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_14_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_14_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_14_END_STABILITY=7.9000
PRIMER_RIGHT_14_END_STABILITY=5.7000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_14=384
PRIMER_PAIR_PENALTY_15=0.2309
PRIMER_LEFT_15_PENALTY=0.108818
PRIMER_RIGHT_15_PENALTY=0.122120
PRIMER_LEFT_15_SEQUENCE=GAGGAGAATATCTTGCTGGGAGT
PRIMER_RIGHT_15_SEQUENCE=TGTCCATCTGTCCCAAGATAAGT
PRIMER_LEFT_15=436,23
PRIMER_RIGHT_15=1000,23
PRIMER_LEFT_15_TM=60.109
PRIMER_RIGHT_15_TM=59.878
PRIMER_LEFT_15_GC_PERCENT=47.826
PRIMER_RIGHT_15_GC_PERCENT=43.478
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_MISPRIMING_SCORE=11.00, MER34 a consensus
PRIMER_RIGHT_15_MISPRIMING_SCORE=12.00, reverse MER41 Medium reiteration sequence MER41
PRIMER_PAIR_15_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_15_END_STABILITY=7.6000
PRIMER_RIGHT_15_END_STABILITY=5.7000
PRIMER_PAIR_15_COMPL_ANY=7.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_15=565
PRIMER_PAIR_PENALTY_16=0.2325
PRIMER_LEFT_16_PENALTY=0.151103
PRIMER_RIGHT_16_PENALTY=0.081398
PRIMER_LEFT_16_SEQUENCE=ATCGTCACCCAAGAAAGATATGA
PRIMER_RIGHT_16_SEQUENCE=GAGAAACTACTGGTGTGGTCCAG
PRIMER_LEFT_16=2406,23
PRIMER_RIGHT_16=2813,23
PRIMER_LEFT_16_TM=59.849
PRIMER_RIGHT_16_TM=60.081
PRIMER_LEFT_16_GC_PERCENT=39.130
PRIMER_RIGHT_16_GC_PERCENT=52.174
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=5.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=3.00
PRIMER_LEFT_16_MISPRIMING_SCORE=12.00, L1PA7 3'-end of L1 repeat (subfamily L1PA7) - a consensus sequence
PRIMER_RIGHT_16_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_16_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_16_END_STABILITY=5.9000
PRIMER_RIGHT_16_END_STABILITY=8.2000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_16=408
PRIMER_PAIR_PENALTY_17=0.2399
PRIMER_LEFT_17_PENALTY=0.151103
PRIMER_RIGHT_17_PENALTY=0.088773
PRIMER_LEFT_17_SEQUENCE=ATCGTCACCCAAGAAAGATATGA
PRIMER_RIGHT_17_SEQUENCE=AAATCAAGATGTAAGGGGTCGAT
PRIMER_LEFT_17=2406,23
PRIMER_RIGHT_17=3323,23
PRIMER_LEFT_17_TM=59.849
PRIMER_RIGHT_17_TM=60.089
PRIMER_LEFT_17_GC_PERCENT=39.130
PRIMER_RIGHT_17_GC_PERCENT=39.130
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=2.00
PRIMER_LEFT_17_MISPRIMING_SCORE=12.00, L1PA7 3'-end of L1 repeat (subfamily L1PA7) - a consensus sequence
PRIMER_RIGHT_17_MISPRIMING_SCORE=12.00, MARINER1 Human mariner
PRIMER_PAIR_17_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_17_END_STABILITY=5.9000
PRIMER_RIGHT_17_END_STABILITY=8.3000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_17=918
PRIMER_PAIR_PENALTY_18=0.2662
PRIMER_LEFT_18_PENALTY=0.151103
PRIMER_RIGHT_18_PENALTY=0.115144
PRIMER_LEFT_18_SEQUENCE=ATCGTCACCCAAGAAAGATATGA
PRIMER_RIGHT_18_SEQUENCE=GCAAGAAGGAAGATTCACTCAAA
PRIMER_LEFT_18=2406,23
PRIMER_RIGHT_18=3124,23
PRIMER_LEFT_18_TM=59.849
PRIMER_RIGHT_18_TM=59.885
PRIMER_LEFT_18_GC_PERCENT=39.130
PRIMER_RIGHT_18_GC_PERCENT=39.130
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_MISPRIMING_SCORE=12.00, L1PA7 3'-end of L1 repeat (subfamily L1PA7) - a consensus sequence
PRIMER_RIGHT_18_MISPRIMING_SCORE=12.00, reverse MER11A a consensus
PRIMER_PAIR_18_MISPRIMING_SCORE=21.00, MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_LEFT_18_END_STABILITY=5.9000
PRIMER_RIGHT_18_END_STABILITY=7.3000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_18=719
PRIMER_PAIR_PENALTY_19=0.2745
PRIMER_LEFT_19_PENALTY=0.166336
PRIMER_RIGHT_19_PENALTY=0.108177
PRIMER_LEFT_19_SEQUENCE=GATCATCTTTATCTCGGCTCTGA
PRIMER_RIGHT_19_SEQUENCE=CTGCGCTTCTGCTTTACATAGTT
PRIMER_LEFT_19=1018,23
PRIMER_RIGHT_19=1361,23
PRIMER_LEFT_19_TM=59.834
PRIMER_RIGHT_19_TM=60.108
PRIMER_LEFT_19_GC_PERCENT=43.478
PRIMER_RIGHT_19_GC_PERCENT=43.478
PRIMER_LEFT_19_SELF_ANY=5.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=3.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_MISPRIMING_SCORE=12.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_19_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_19_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_19_END_STABILITY=6.7000
PRIMER_RIGHT_19_END_STABILITY=5.7000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_19=344
=