PRIMER_SEQUENCE_ID=NM_021090
SEQUENCE=GTGAGAAGGCGGCGGCGGCGGCGGAGTAGCAGCCGGACGAGACGTCCCAGCGGCTCAGGCGCTGCCCAGCGCCGGCCCTGGCAGGGAGCCTGGTGGGGTGGCGGAGGGGGAGACTGTGCCGTGGAGGGCCTCGCCATGTCCTGCTGCCCGACTTCCTTGTGAAACCTCCTAGTAGAAAAAGTTCTTGGGACAAACTTCATTTGACTTCACCATAAGGGAAAGAAGAGAGCCTTGTTGGACACTGAAGAAGGGACGGGGATTTCTCCTCCTAATCTGGGCCTCTTGTCATGGATGAAGAGACTCGGCACAGCCTTGAGTGCATCCAGGCCAATCAGATCTTTCCCAGGAAGCAGCTGATCCGGGAGGATGAGAATCTTCAGGTTCCTTTCCTTGAACTTCATGGAGAGAGCACAGAGTTTGTGGGCCGTGCCGAGGATGCCATCATTGCCCTTTCCAATTACAGACTTCACATCAAGTTCAAGGAGTCTCTTGTTAATGTTCCATTACAGCTTATAGAAAGTGTTGAATGCCGAGATATATTTCAGCTTCATTTGACTTGCAAAGACTGCAAAGTTATCAGGTGTCAGTTTTCAACCTTTGAGCAGTGTCAAGAGTGGCTGAAGAGACTGAACAACGCAATCCGACCACCTGCTAAAATAGAAGATCTCTTCTCATTTGCATACCATGCTTGGTGCATGGAGGTCTATGCCAGTGAAAAAGAGCAACATGGAGACCTGTGCAGACCAGGGGAGCATGTAACTTCAAGGTTTAAAAACGAGGTGGAGAGGATGGGTTTTGATATGAACAACGCCTGGAGGATTTCCAACATCAATGAGAAGTACAAATTATGTGGTAGCTATCCTCAAGAGCTCATAGTGCCTGCCTGGATCACTGACAAAGAACTGGAAAGTGTATCAAGTTTCAGGTCCTGGAAGCGCATCCCTGCCGTCATCTACAGGCACCAGAGCAATGGAGCTGTCATTGCCCGCTGTGGACAGCCAGAGGTTAGCTGGTGGGGCTGGCGAAATGCAGATGATGAGCATCTGGTACAGTCAGTAGCCAAAGCTTGTGCCTCTGACTCCCGATCGAGTGGCAGCAAGCTGTCAACTAGGAACACTTCTCGAGACTTTCCCAATGGGGGAGACCTTTCTGACGTGGAGTTCGATTCTTCTCTGTCAAATGCTTCAGGAGCAGAGAGTTTAGCCATCCAACCGCAGAAGCTTTTGATCTTGGATGCACGCTCCTATGCAGCTGCTGTGGCAAACCGAGCCAAAGGAGGAGGCTGCGAATGCCCAGAGTATTACCCAAACTGTGAAGTTGTGTTTATGGGGATGGCAAACATTCATTCTATTCGGAGGAGTTTTCAGTCTCTGCGGTTGCTGTGCACTCAGATGCCAGATCCGGGAAATTGGCTATCAGCTCTTGAAAGCACAAAATGGCTCCATCACTTGTCTGTGCTTCTGAAATCAGCGCTTCTGGTAGTGCATGCTGTGGATCAGGATCAGCGGCCGGTGCTAGTACACTGCTCAGATGGCTGGGACCGCACCCCCCAGATTGTGGCATTGGCTAAGCTCTTGCTGGACCCTTATTACCGAACCATAGAGGGTTTCCAGGTCCTCGTGGAAATGGAGTGGCTGGATTTTGGCCATAAATTTGCTGACCGGTGTGGTCATGGGGAGAACTCGGATGATCTGAATGAACGTTGCCCAGTGTTTCTGCAGTGGCTTGACTGTGTTCATCAGCTTCAGAGGCAATTTCCTTGCTCTTTTGAGTTCAATGAAGCATTCCTTGTGAAACTGGTGCAGCATACCTATTCCTGCCTGTTTGGAACATTCCTGTGCAACAACGCCAAGGAGAGAGGGGAAAAGCATACTCAGGAACGGACATGTTCCGTGTGGTCACTTCTTCGGGCAGGCAACAAGGCTTTCAAAAACCTACTGTATTCCTCTCAGTCAGAAGCCGTGCTGTACCCTGTGTGCCATGTGCGTAACCTGATGCTGTGGAGTGCAGTGTACCTGCCCTGCCCATCCCCAACCACCCCTGTGGACGACAGCTGTGCACCATACCCAGCCCCAGGCACCAGCCCTGATGATCCCCCCCTGAGCCGGCTACCAAAGACTAGATCATACGACAATCTGACCACAGCCTGTGACAACACAGTGCCTCTGGCCAGCCGGCGCTGCAGCGACCCCAGCCTGAACGAGAAGTGGCAGGAGCACCGGCGCTCACTAGAGCTGAGCAGCCTGGCTGGCCCTGGAGAGGATCCCCTTTCTGCCGACAGCCTAGGGAAGCCCACCAGAGTGCCGGGGGGTGCCGAGCTTTCTGTTGCAGCCGGAGTAGCTGAGGGGCAGATGGAGAACATCTTGCAGGAGGCCACCAAAGAGGAGAGTGGAGTAGAGGAACCTGCCCACAGGGCAGGCATTGAGATACAGGAGGGTAAAGAGGACCCTCTCTTAGAAAAGGAGAGCAGGAGGAAGACACCTGAGGCCTCAGCCATTGGACTTCACCAAGACCCAGAACTGGGTGATGCTGCTCTGAGGAGCCATCTGGATATGAGCTGGCCTCTGTTCTCACAGGGCATTTCTGAACAGCAGAGTGGGCTCAGTGTTCTCCTCAGTTCTCTCCAGGTCCCCCCCAGGGGAGAGGATTCCCTGGAGGTCCCTGTGGAGCAGTTTCGAATAGAAGAGATTGCAGAGGGTAGGGAGGAAGCAGTTCTTCCAATCCCAGTAGATGCAAAAGTTGGCTATGGTACCTCACAGTCATGTTCTCTGCTACCTTCCCAAGTCCCTTTTGAGACCAGAGGACCAAACGTGGACAGTTCTACAGACATGTTAGTGGAAGATAAGGTGAAGTCAGTAAGTGGGCCCCAAGGTCATCATAGATCTTGCCTTGTAAATAGTGGCAAGGACAGGCTTCCTCAGACCATGGAACCCAGCCCTTCAGAGACAAGCCTGGTCGAGAGGCCCCAAGTGGGGTCTGTGGTGCATAGGACTTCCCTTGGCAGCACTCTCAGCCTGACACGTTCCCCTTGTGCCTTGCCTTTAGCCGAATGTAAAGAGGGGCTTGTGTGCAATGGTGCCCCAGAGACTGAAAACAGGGCCTCAGAGCAGCCCCCAGGTCTTAGCACCCTCCAGATGTACCCCACACCCAATGGGCATTGCGCCAATGGGGAGGCTGGTAGGAGCAAGGACTCACTGAGCCGTCAGCTGTCTGCTATGAGCTGCAGCTCTGCCCACTTACACTCAAGGAACTTGCACCACAAGTGGCTGCATAGCCACTCAGGAAGGCCATCTGCAACCAGCAGCCCCGACCAGCCTTCCCGCAGCCACCTGGACGATGATGGCATGTCAGTGTACACAGACACGATCCAACAGCGCCTGCGTCAGATTGAGTCAGGCCACCAGCAGGAAGTAGAAACTTTGAAGAAACAAGTCCAGGAGCTGAAGAGTCGCCTGGAGAGCCAGTACCTGACCAGCTCCCTACACTTTAATGGAGACTTTGGGGATGAGGTGACTTCAATCCCCGACTCGGAAAGCAATCTGGATCAGAACTGTTTGTCTCGCTGCAGCACAGAGATTTTCTCTGAAGCCAGCTGGGAGCAGGTGGATAAACAGGACACAGAGATGACCCGTTGGCTTCCTGACCACCTGGCCGCCCACTGCTATGCGTGCGACAGTGCCTTCTGGCTTGCCAGCAGGAAGCACCACTGCAGGAATTGTGGGAACGTATTCTGCTCCAGTTGTTGTAACCAGAAGGTTCCAGTTCCCAGCCAGCAGCTCTTTGAACCCAGTCGAGTATGCAAGTCTTGCTATAGCAGCCTACATCCCACAAGCTCCAGCATTGACCTTGAACTGGATAAGCCCATTGCTGCCACTTCCAACTGAAGCTCAGTGACCTGGGTGGGCAGTGGCCAAGCTGCTGTTCCTATGACAGGCCCACTCAACCTGGGCAGACCGAGAGGCCCGTGCACTTTGGAATGGGAGCGTGGAACCACCTGTACAGAGTGACAGATTTGGGATGCACCACTGGATTGTAGATTGATTTTTCTTTCCTGTCCCCCTACTCCCTCCCTACCTTTTCCATCCTCCTCCTCTGCCTTCAAAAAAGGAAACTTTCCCTTGGTTGTCTTAATTTTTTTTTTTTTTGATGGAAGACCAAGGGTTGCCAGGCCCACTGTAACTGCCGAGCTGCCTGCTGTCACGTGACACTGAGGGATGGCTTGTTTCTTCCGGGTGGGAGGATGGTGGTCAGAGCCAGGAGTATGGAGATCTGAGACCGTGAGCAGGGAAGAAAGCCAGTGCTAACATGCAACCATTCCTCACGCCACCGCCACATCAGAGCTGGTTGGGAACCCTTTGCTGCTGGGGAGGCTGGAAGCATATTCCCCAAGAGCACTGCCCTGGGCATCATCTCCCTCCTGCGAGGAGCTGAGCCAGTCCCCTCACAGATGGATAAATGAGGCTGATGTTTGGAGGGAGAGGCACACGGTAAATGGCATCCCCTTAGGCGCCTCTTTGGGAAAGGAAAGTGGATGCTCCTTTGAGGCAGGCGAGGGGCTGGGAGTGGGTAGCCGTCATGTTGTCCCCCGTGGGATCCCATTTTTAACTTGACCCAGATTGTCTTGGGCTCCTTTTACTTTCAGGGGGCTGCTTTCCTGGGCCAGGCTGTGTAGCACTTCCCACCCTCAGGCATGAGTACAGACTGGTCAAAATGTTTATGCAGTCAAGGCCAAAGCCTGCCCTGAGCCCAGACACACCTGGGTCCCCATTCTGGGGCCTGGTCCCCTAAACAATCTCTTCCTCAGCCAGCACAGGGAAATCCAGACTCAGGGTTCCAGAAATCCCCCGTTCCCAAACCAAACCAATCATGGATCTTCCCTTTAAGGGGTAGAATCAGCCTTTAGAGTTACAAGCCCCTGGAAAAGGGAGCAGGTCCCATACAATCAGCCTTTCTCCTCTTCTCTTCTTGGGACAGGAATAACTGCTGAGCAGTCCCCCTTTGCCACTCCTGGCTGTCTGAGTGGGCACTTGTCTGGCTGCTGGCCAGCAGTGAAGGGCCACTTGGTGACTTCGTAGGGTTCCTTAGAGAAGTGACATGGCCTTGAGGAGATTCAGGGCACCTTTCCTCAGTGAGCTTTGATATGGTCCAAAAACAGCCTTAAATCAAGAATATTTGTGGAGGTAGGTGTGGGGAAGGTTGGAGAAGAGTTAGGTTTGTGCTTTGTAATCTTGGCATCCATGTTTTGTGCCTGCCCTCCCTCTTGGGGAGGCCATGCTTGACTCTGCTGAAAGCTGCTAACTGGTGACAGGGTGGATCATGGTGAGGACTAGGGGTAAGGTCAGGGAATGCTTAAGCTCTGGCCCTGCCCTGTATTCCTCTTCCCCTTGGTAGCAGTTCCCTGACCAAGGGCAAGGTGTGTCTCAGGAAGGTGGTTCTGTGCATGCCTGGGTGTGGTTAAGGCGTGGCCCAGAAGGCTTCCCTGGTGCTCTCAGTCCAGGCAAAGCCCAGAGCATCTGAGCACGTCTCTGACTTCCAGTGGCAACACAGTTTGAACGTGGTGAAACAGGGTGTAGTTCTTTTCCACATTCTGTGTCATCTAGTCGTGCAGGTAGGGAAAAAGTTGGTCTAATTGAAGATTGTGCATTTCCTAGTGACAGGTGCCAAGAGGTTATGATACGGGTTTCTTGGGTCTGATGTACAGTGTGAATAAATGCTTGCAGCTCTTAGCTCTTTTTTGATCGATGAAGCACTTTTTTATTAATATTTTCCTTTGTTAAAGGAGGAACCGTAACTCTCCATAGCTGTACATATAACCCTTTTCTCCTAAAGAGGAGTCAGTCAGTGCTCCTATATTTTTCATTTTTTGTCAAAGCAAGAAGTAAATACTTTAGAATTGTTAAATATATAAATAAAGCAAATAAAGTTGAGTGTTCCACATGGTAGCATTTGCTAACACTTCCTAA
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 69016, GC content failed 923, low tm 24381, high tm 28183, high any compl 11, high end compl 33, high repeat similarity 45, long poly-x seq 184, ok 15256
PRIMER_RIGHT_EXPLAIN=considered 69034, GC content failed 919, low tm 24803, high tm 27755, high any compl 3, high end compl 4, high repeat similarity 9, long poly-x seq 185, ok 15356
PRIMER_PAIR_EXPLAIN=considered 1009, unacceptable product size 951, high end compl 6, ok 52
PRIMER_PAIR_PENALTY=0.0138
PRIMER_LEFT_PENALTY=0.012719
PRIMER_RIGHT_PENALTY=0.001065
PRIMER_LEFT_SEQUENCE=TGACTTCACCATAAGGGAAAGAA
PRIMER_RIGHT_SEQUENCE=AGTGTTCCTAGTTGACAGCTTGC
PRIMER_LEFT=201,23
PRIMER_RIGHT=1117,23
PRIMER_LEFT_TM=59.987
PRIMER_RIGHT_TM=59.999
PRIMER_LEFT_GC_PERCENT=39.130
PRIMER_RIGHT_GC_PERCENT=47.826
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=12.00, LTR3 LTR from human DNA related to mouse mammary tumor virus (MMTV) 3' LTR
PRIMER_PAIR_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=917
PRIMER_PAIR_PENALTY_1=0.0139
PRIMER_LEFT_1_PENALTY=0.002480
PRIMER_RIGHT_1_PENALTY=0.011391
PRIMER_LEFT_1_SEQUENCE=CCTTTCTCCTCTTCTCTTCTTGG
PRIMER_RIGHT_1_SEQUENCE=GGAGAGTTACGGTTCCTCCTTTA
PRIMER_LEFT_1=4946,23
PRIMER_RIGHT_1=5796,23
PRIMER_LEFT_1_TM=60.002
PRIMER_RIGHT_1_TM=60.011
PRIMER_LEFT_1_GC_PERCENT=47.826
PRIMER_RIGHT_1_GC_PERCENT=47.826
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_1_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_1_MISPRIMING_SCORE=21.00, reverse MER39 a consensus
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=6.3000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=851
PRIMER_PAIR_PENALTY_2=0.0270
PRIMER_LEFT_2_PENALTY=0.025930
PRIMER_RIGHT_2_PENALTY=0.001065
PRIMER_LEFT_2_SEQUENCE=AGTGGCTGAAGAGACTGAACAAC
PRIMER_RIGHT_2_SEQUENCE=AGTGTTCCTAGTTGACAGCTTGC
PRIMER_LEFT_2=612,23
PRIMER_RIGHT_2=1117,23
PRIMER_LEFT_2_TM=59.974
PRIMER_RIGHT_2_TM=59.999
PRIMER_LEFT_2_GC_PERCENT=47.826
PRIMER_RIGHT_2_GC_PERCENT=47.826
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, L1PA2 3'-end of L1 repeat (subfamily L1PA2) - a consensus sequence
PRIMER_RIGHT_2_MISPRIMING_SCORE=12.00, LTR3 LTR from human DNA related to mouse mammary tumor virus (MMTV) 3' LTR
PRIMER_PAIR_2_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=506
PRIMER_PAIR_PENALTY_3=0.0429
PRIMER_LEFT_3_PENALTY=0.025930
PRIMER_RIGHT_3_PENALTY=0.016975
PRIMER_LEFT_3_SEQUENCE=AGTGGCTGAAGAGACTGAACAAC
PRIMER_RIGHT_3_SEQUENCE=CTCCGAATAGAATGAATGTTTGC
PRIMER_LEFT_3=612,23
PRIMER_RIGHT_3=1356,23
PRIMER_LEFT_3_TM=59.974
PRIMER_RIGHT_3_TM=59.983
PRIMER_LEFT_3_GC_PERCENT=47.826
PRIMER_RIGHT_3_GC_PERCENT=39.130
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, L1PA2 3'-end of L1 repeat (subfamily L1PA2) - a consensus sequence
PRIMER_RIGHT_3_MISPRIMING_SCORE=11.00, reverse L1MA5 3'-end of L1 repeat (subfamily L1MA5) - a consensus sequence
PRIMER_PAIR_3_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=8.8000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=745
PRIMER_PAIR_PENALTY_4=0.0520
PRIMER_LEFT_4_PENALTY=0.002480
PRIMER_RIGHT_4_PENALTY=0.049500
PRIMER_LEFT_4_SEQUENCE=CCTTTCTCCTCTTCTCTTCTTGG
PRIMER_RIGHT_4_SEQUENCE=TGTGGAAAAGAACTACACCCTGT
PRIMER_LEFT_4=4946,23
PRIMER_RIGHT_4=5584,23
PRIMER_LEFT_4_TM=60.002
PRIMER_RIGHT_4_TM=59.950
PRIMER_LEFT_4_GC_PERCENT=47.826
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, reverse MER11A a consensus
PRIMER_PAIR_4_MISPRIMING_SCORE=21.00, reverse L1MA2 3'-end of L1 repeat (subfamily L1MA2) - a consensus sequence
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=639
PRIMER_PAIR_PENALTY_5=0.0520
PRIMER_LEFT_5_PENALTY=0.002480
PRIMER_RIGHT_5_PENALTY=0.049500
PRIMER_LEFT_5_SEQUENCE=CCTTTCTCCTCTTCTCTTCTTGG
PRIMER_RIGHT_5_SEQUENCE=AAGAACTACACCCTGTTTCACCA
PRIMER_LEFT_5=4946,23
PRIMER_RIGHT_5=5577,23
PRIMER_LEFT_5_TM=60.002
PRIMER_RIGHT_5_TM=59.950
PRIMER_LEFT_5_GC_PERCENT=47.826
PRIMER_RIGHT_5_GC_PERCENT=43.478
PRIMER_LEFT_5_SELF_ANY=2.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=2.00
PRIMER_LEFT_5_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_5_MISPRIMING_SCORE=12.00, MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_PAIR_5_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_5_END_STABILITY=8.5000
PRIMER_RIGHT_5_END_STABILITY=8.2000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_5=632
PRIMER_PAIR_PENALTY_6=0.0541
PRIMER_LEFT_6_PENALTY=0.053000
PRIMER_RIGHT_6_PENALTY=0.001065
PRIMER_LEFT_6_SEQUENCE=GCTTCATTTGACTTGCAAAGACT
PRIMER_RIGHT_6_SEQUENCE=AGTGTTCCTAGTTGACAGCTTGC
PRIMER_LEFT_6=544,23
PRIMER_RIGHT_6=1117,23
PRIMER_LEFT_6_TM=59.947
PRIMER_RIGHT_6_TM=59.999
PRIMER_LEFT_6_GC_PERCENT=39.130
PRIMER_RIGHT_6_GC_PERCENT=47.826
PRIMER_LEFT_6_SELF_ANY=6.00
PRIMER_RIGHT_6_SELF_ANY=5.00
PRIMER_LEFT_6_SELF_END=3.00
PRIMER_RIGHT_6_SELF_END=3.00
PRIMER_LEFT_6_MISPRIMING_SCORE=11.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_6_MISPRIMING_SCORE=12.00, LTR3 LTR from human DNA related to mouse mammary tumor virus (MMTV) 3' LTR
PRIMER_PAIR_6_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_6_END_STABILITY=6.1000
PRIMER_RIGHT_6_END_STABILITY=8.5000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_6=574
PRIMER_PAIR_PENALTY_7=0.0567
PRIMER_LEFT_7_PENALTY=0.012719
PRIMER_RIGHT_7_PENALTY=0.044016
PRIMER_LEFT_7_SEQUENCE=TGACTTCACCATAAGGGAAAGAA
PRIMER_RIGHT_7_SEQUENCE=CTCTCCACCTCGTTTTTAAACCT
PRIMER_LEFT_7=201,23
PRIMER_RIGHT_7=786,23
PRIMER_LEFT_7_TM=59.987
PRIMER_RIGHT_7_TM=60.044
PRIMER_LEFT_7_GC_PERCENT=39.130
PRIMER_RIGHT_7_GC_PERCENT=43.478
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=2.00
PRIMER_RIGHT_7_SELF_END=3.00
PRIMER_LEFT_7_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_7_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_7_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_7_END_STABILITY=7.0000
PRIMER_RIGHT_7_END_STABILITY=7.9000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_7=586
PRIMER_PAIR_PENALTY_8=0.0576
PRIMER_LEFT_8_PENALTY=0.018314
PRIMER_RIGHT_8_PENALTY=0.039247
PRIMER_LEFT_8_SEQUENCE=GGACCCTTATTACCGAACCATAG
PRIMER_RIGHT_8_SEQUENCE=GTTCATTCAGATCATCCGAGTTC
PRIMER_LEFT_8=1579,23
PRIMER_RIGHT_8=1700,23
PRIMER_LEFT_8_TM=59.982
PRIMER_RIGHT_8_TM=59.961
PRIMER_LEFT_8_GC_PERCENT=47.826
PRIMER_RIGHT_8_GC_PERCENT=43.478
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=7.00
PRIMER_LEFT_8_SELF_END=2.00
PRIMER_RIGHT_8_SELF_END=3.00
PRIMER_LEFT_8_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_8_MISPRIMING_SCORE=24.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_8_END_STABILITY=5.9000
PRIMER_RIGHT_8_END_STABILITY=6.4000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_8=122
PRIMER_PAIR_PENALTY_9=0.0609
PRIMER_LEFT_9_PENALTY=0.049500
PRIMER_RIGHT_9_PENALTY=0.011391
PRIMER_LEFT_9_SEQUENCE=ACAGGGTGTAGTTCTTTTCCACA
PRIMER_RIGHT_9_SEQUENCE=GGAGAGTTACGGTTCCTCCTTTA
PRIMER_LEFT_9=5562,23
PRIMER_RIGHT_9=5796,23
PRIMER_LEFT_9_TM=59.950
PRIMER_RIGHT_9_TM=60.011
PRIMER_LEFT_9_GC_PERCENT=43.478
PRIMER_RIGHT_9_GC_PERCENT=47.826
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=5.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=2.00
PRIMER_LEFT_9_MISPRIMING_SCORE=12.00, reverse MER11A a consensus
PRIMER_RIGHT_9_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_9_MISPRIMING_SCORE=21.00, reverse MER11A a consensus
PRIMER_LEFT_9_END_STABILITY=8.2000
PRIMER_RIGHT_9_END_STABILITY=6.3000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_9=235
PRIMER_PAIR_PENALTY_10=0.0609
PRIMER_LEFT_10_PENALTY=0.049500
PRIMER_RIGHT_10_PENALTY=0.011391
PRIMER_LEFT_10_SEQUENCE=TGGTGAAACAGGGTGTAGTTCTT
PRIMER_RIGHT_10_SEQUENCE=GGAGAGTTACGGTTCCTCCTTTA
PRIMER_LEFT_10=5555,23
PRIMER_RIGHT_10=5796,23
PRIMER_LEFT_10_TM=59.950
PRIMER_RIGHT_10_TM=60.011
PRIMER_LEFT_10_GC_PERCENT=43.478
PRIMER_RIGHT_10_GC_PERCENT=47.826
PRIMER_LEFT_10_SELF_ANY=3.00
PRIMER_RIGHT_10_SELF_ANY=5.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=2.00
PRIMER_LEFT_10_MISPRIMING_SCORE=11.00, reverse MER11A a consensus
PRIMER_RIGHT_10_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_10_MISPRIMING_SCORE=20.00, reverse MER4C a consensus
PRIMER_LEFT_10_END_STABILITY=7.0000
PRIMER_RIGHT_10_END_STABILITY=6.3000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_10=242
PRIMER_PAIR_PENALTY_11=0.0621
PRIMER_LEFT_11_PENALTY=0.061028
PRIMER_RIGHT_11_PENALTY=0.001065
PRIMER_LEFT_11_SEQUENCE=TCAGGTGTCAGTTTTCAACCTTT
PRIMER_RIGHT_11_SEQUENCE=AGTGTTCCTAGTTGACAGCTTGC
PRIMER_LEFT_11=576,23
PRIMER_RIGHT_11=1117,23
PRIMER_LEFT_11_TM=60.061
PRIMER_RIGHT_11_TM=59.999
PRIMER_LEFT_11_GC_PERCENT=39.130
PRIMER_RIGHT_11_GC_PERCENT=47.826
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=5.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=3.00
PRIMER_LEFT_11_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, LTR3 LTR from human DNA related to mouse mammary tumor virus (MMTV) 3' LTR
PRIMER_PAIR_11_MISPRIMING_SCORE=22.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_11_END_STABILITY=8.5000
PRIMER_RIGHT_11_END_STABILITY=8.5000
PRIMER_PAIR_11_COMPL_ANY=6.00
PRIMER_PAIR_11_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_11=542
PRIMER_PAIR_PENALTY_12=0.0621
PRIMER_LEFT_12_PENALTY=0.061028
PRIMER_RIGHT_12_PENALTY=0.001065
PRIMER_LEFT_12_SEQUENCE=GGTGTCAGTTTTCAACCTTTGAG
PRIMER_RIGHT_12_SEQUENCE=AGTGTTCCTAGTTGACAGCTTGC
PRIMER_LEFT_12=579,23
PRIMER_RIGHT_12=1117,23
PRIMER_LEFT_12_TM=60.061
PRIMER_RIGHT_12_TM=59.999
PRIMER_LEFT_12_GC_PERCENT=43.478
PRIMER_RIGHT_12_GC_PERCENT=47.826
PRIMER_LEFT_12_SELF_ANY=5.00
PRIMER_RIGHT_12_SELF_ANY=5.00
PRIMER_LEFT_12_SELF_END=3.00
PRIMER_RIGHT_12_SELF_END=3.00
PRIMER_LEFT_12_MISPRIMING_SCORE=11.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_12_MISPRIMING_SCORE=12.00, LTR3 LTR from human DNA related to mouse mammary tumor virus (MMTV) 3' LTR
PRIMER_PAIR_12_MISPRIMING_SCORE=22.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_12_END_STABILITY=7.0000
PRIMER_RIGHT_12_END_STABILITY=8.5000
PRIMER_PAIR_12_COMPL_ANY=6.00
PRIMER_PAIR_12_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_12=539
PRIMER_PAIR_PENALTY_13=0.0646
PRIMER_LEFT_13_PENALTY=0.028128
PRIMER_RIGHT_13_PENALTY=0.036444
PRIMER_LEFT_13_SEQUENCE=TGGAGCAGTTTCGAATAGAAGAG
PRIMER_RIGHT_13_SEQUENCE=TGCCACTATTTACAAGGCAAGAT
PRIMER_LEFT_13=2664,23
PRIMER_RIGHT_13=2903,23
PRIMER_LEFT_13_TM=60.028
PRIMER_RIGHT_13_TM=60.036
PRIMER_LEFT_13_GC_PERCENT=43.478
PRIMER_RIGHT_13_GC_PERCENT=39.130
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=2.00
PRIMER_LEFT_13_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_13_MISPRIMING_SCORE=12.00, reverse L1MA2 3'-end of L1 repeat (subfamily L1MA2) - a consensus sequence
PRIMER_PAIR_13_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_13_END_STABILITY=6.7000
PRIMER_RIGHT_13_END_STABILITY=6.6000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_13=240
PRIMER_PAIR_PENALTY_14=0.0649
PRIMER_LEFT_14_PENALTY=0.002480
PRIMER_RIGHT_14_PENALTY=0.062402
PRIMER_LEFT_14_SEQUENCE=CCTTTCTCCTCTTCTCTTCTTGG
PRIMER_RIGHT_14_SEQUENCE=AAGAGCTGCAAGCATTTATTCAC
PRIMER_LEFT_14=4946,23
PRIMER_RIGHT_14=5724,23
PRIMER_LEFT_14_TM=60.002
PRIMER_RIGHT_14_TM=59.938
PRIMER_LEFT_14_GC_PERCENT=47.826
PRIMER_RIGHT_14_GC_PERCENT=39.130
PRIMER_LEFT_14_SELF_ANY=2.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_14_MISPRIMING_SCORE=12.00, reverse L1MA5 3'-end of L1 repeat (subfamily L1MA5) - a consensus sequence
PRIMER_PAIR_14_MISPRIMING_SCORE=21.00, reverse L1MA5 3'-end of L1 repeat (subfamily L1MA5) - a consensus sequence
PRIMER_LEFT_14_END_STABILITY=8.5000
PRIMER_RIGHT_14_END_STABILITY=6.7000
PRIMER_PAIR_14_COMPL_ANY=6.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_14=779
PRIMER_PAIR_PENALTY_15=0.0671
PRIMER_LEFT_15_PENALTY=0.017590
PRIMER_RIGHT_15_PENALTY=0.049500
PRIMER_LEFT_15_SEQUENCE=AGATTGTCTTGGGCTCCTTTTAC
PRIMER_RIGHT_15_SEQUENCE=AAGAACTACACCCTGTTTCACCA
PRIMER_LEFT_15=4613,23
PRIMER_RIGHT_15=5577,23
PRIMER_LEFT_15_TM=60.018
PRIMER_RIGHT_15_TM=59.950
PRIMER_LEFT_15_GC_PERCENT=43.478
PRIMER_RIGHT_15_GC_PERCENT=43.478
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=2.00
PRIMER_LEFT_15_MISPRIMING_SCORE=12.00, reverse L1MB7 3'-end of L1 repeat (subfamily L1MB7) - a consensus sequence
PRIMER_RIGHT_15_MISPRIMING_SCORE=12.00, MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_PAIR_15_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_15_END_STABILITY=6.0000
PRIMER_RIGHT_15_END_STABILITY=8.2000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_15=965
PRIMER_PAIR_PENALTY_16=0.0671
PRIMER_LEFT_16_PENALTY=0.017590
PRIMER_RIGHT_16_PENALTY=0.049500
PRIMER_LEFT_16_SEQUENCE=AGATTGTCTTGGGCTCCTTTTAC
PRIMER_RIGHT_16_SEQUENCE=TGTGGAAAAGAACTACACCCTGT
PRIMER_LEFT_16=4613,23
PRIMER_RIGHT_16=5584,23
PRIMER_LEFT_16_TM=60.018
PRIMER_RIGHT_16_TM=59.950
PRIMER_LEFT_16_GC_PERCENT=43.478
PRIMER_RIGHT_16_GC_PERCENT=43.478
PRIMER_LEFT_16_SELF_ANY=3.00
PRIMER_RIGHT_16_SELF_ANY=3.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=3.00
PRIMER_LEFT_16_MISPRIMING_SCORE=12.00, reverse L1MB7 3'-end of L1 repeat (subfamily L1MB7) - a consensus sequence
PRIMER_RIGHT_16_MISPRIMING_SCORE=12.00, reverse MER11A a consensus
PRIMER_PAIR_16_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_16_END_STABILITY=6.0000
PRIMER_RIGHT_16_END_STABILITY=7.9000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_16=972
PRIMER_PAIR_PENALTY_17=0.0729
PRIMER_LEFT_17_PENALTY=0.036444
PRIMER_RIGHT_17_PENALTY=0.036444
PRIMER_LEFT_17_SEQUENCE=TAGATGCAAAAGTTGGCTATGGT
PRIMER_RIGHT_17_SEQUENCE=TGCCACTATTTACAAGGCAAGAT
PRIMER_LEFT_17=2727,23
PRIMER_RIGHT_17=2903,23
PRIMER_LEFT_17_TM=60.036
PRIMER_RIGHT_17_TM=60.036
PRIMER_LEFT_17_GC_PERCENT=39.130
PRIMER_RIGHT_17_GC_PERCENT=39.130
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=2.00
PRIMER_LEFT_17_MISPRIMING_SCORE=11.00, reverse L1MA2 3'-end of L1 repeat (subfamily L1MA2) - a consensus sequence
PRIMER_RIGHT_17_MISPRIMING_SCORE=12.00, reverse L1MA2 3'-end of L1 repeat (subfamily L1MA2) - a consensus sequence
PRIMER_PAIR_17_MISPRIMING_SCORE=23.00, reverse L1MA2 3'-end of L1 repeat (subfamily L1MA2) - a consensus sequence
PRIMER_LEFT_17_END_STABILITY=7.8000
PRIMER_RIGHT_17_END_STABILITY=6.6000
PRIMER_PAIR_17_COMPL_ANY=6.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_17=177
PRIMER_PAIR_PENALTY_18=0.0729
PRIMER_LEFT_18_PENALTY=0.036444
PRIMER_RIGHT_18_PENALTY=0.036444
PRIMER_LEFT_18_SEQUENCE=GTAGATGCAAAAGTTGGCTATGG
PRIMER_RIGHT_18_SEQUENCE=TGCCACTATTTACAAGGCAAGAT
PRIMER_LEFT_18=2726,23
PRIMER_RIGHT_18=2903,23
PRIMER_LEFT_18_TM=60.036
PRIMER_RIGHT_18_TM=60.036
PRIMER_LEFT_18_GC_PERCENT=43.478
PRIMER_RIGHT_18_GC_PERCENT=39.130
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=2.00
PRIMER_RIGHT_18_SELF_END=2.00
PRIMER_LEFT_18_MISPRIMING_SCORE=11.00, L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence
PRIMER_RIGHT_18_MISPRIMING_SCORE=12.00, reverse L1MA2 3'-end of L1 repeat (subfamily L1MA2) - a consensus sequence
PRIMER_PAIR_18_MISPRIMING_SCORE=23.00, reverse L1MA2 3'-end of L1 repeat (subfamily L1MA2) - a consensus sequence
PRIMER_LEFT_18_END_STABILITY=7.4000
PRIMER_RIGHT_18_END_STABILITY=6.6000
PRIMER_PAIR_18_COMPL_ANY=6.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_18=178
PRIMER_PAIR_PENALTY_19=0.0729
PRIMER_LEFT_19_PENALTY=0.036444
PRIMER_RIGHT_19_PENALTY=0.036444
PRIMER_LEFT_19_SEQUENCE=AGATGCAAAAGTTGGCTATGGTA
PRIMER_RIGHT_19_SEQUENCE=TGCCACTATTTACAAGGCAAGAT
PRIMER_LEFT_19=2728,23
PRIMER_RIGHT_19=2903,23
PRIMER_LEFT_19_TM=60.036
PRIMER_RIGHT_19_TM=60.036
PRIMER_LEFT_19_GC_PERCENT=39.130
PRIMER_RIGHT_19_GC_PERCENT=39.130
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=2.00
PRIMER_RIGHT_19_SELF_END=2.00
PRIMER_LEFT_19_MISPRIMING_SCORE=11.00, reverse MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_19_MISPRIMING_SCORE=12.00, reverse L1MA2 3'-end of L1 repeat (subfamily L1MA2) - a consensus sequence
PRIMER_PAIR_19_MISPRIMING_SCORE=22.00, reverse L1MA2 3'-end of L1 repeat (subfamily L1MA2) - a consensus sequence
PRIMER_LEFT_19_END_STABILITY=7.2000
PRIMER_RIGHT_19_END_STABILITY=6.6000
PRIMER_PAIR_19_COMPL_ANY=6.00
PRIMER_PAIR_19_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_19=176
=