PRIMER_SEQUENCE_ID=NM_022648
SEQUENCE=CTGCTCCTTGCACCCCGCTCCCTGCCTGGACACAGGCTCACTCGCTGCCTTCTTCTGGGGGAAACCAGCTTCTTGCCAGCCACAGCTGCTGCCTCCGCCACTGGCCACCGCCCCTGTCCTGGGAGTCCCTTGGCCCAGACACCCACCTGACTTAGTGGCTCCTCTGCAGGAAAGGTGGCTGCCCCCTGCGTTCCTCCATCCAACCATGAGCTGGTGCCCATCACCACTGAGAATGCACCAAAGAATGTAGTGGACAAGGGAGAAGGAGCCTCCCGGGGTGGAAACACACGGAAAAGCCTCGAGGACAACGGCTCCACCAGGGTCACCCCGAGTGTCCAGCCCCACCTCCAGCCCATCAGAAACATGAGTGTGAGCCGGACCATGGAGGACAGCTGTGAGCTGGACCTGGTGTACGTCACAGAGAGGATCATCGCTGTCTCCTTCCCCAGCACAGCCAATGAGGAGAACTTCCGGAGCAACCTCCGTGAGGTGGCGCAGATGCTCAAGTCCAAACATGGAGGCAACTACCTGCTGTTCAACCTCTCTGAGCGGAGACCTGACATCACGAAGCTCCATGCCAAGGTACTGGAATTTGGCTGGCCCGACCTCCACACCCCAGCCCTGGAGAAGATCTGCAGCATCTGTAAGGCCATGGACACATGGCTCAATGCAGACCCTCACAATGTCGTTGTTCTACACAACAAGGGAAACCGAGGCAGGATAGGAGTTGTCATCGCGGCTTACATGCACTACAGCAACATTTCTGCCAGTGCGGACCAGGCTCTGGACCGGTTTGCAATGAAGCGGTTCTATGAGGATAAGATTGTGCCCATTGGCCAGCCATCCCAAAGAAGGTACGTGCATTACTTCAGTGGCCTGCTCTCCGGCTCCATCAAAATGAACAACAAGCCCTTGTTTCTGCACCACGTGATCATGCACGGCATCCCCAACTTTGAGTCTAAAGGAGGATGTCGGCCATTTCTCCGCATCTACCAGGCCATGCAACCTGTGTACACATCTGGCATCTACAACATCCCAGGAGACAGCCAGACTAGCGTCTGCATCACCATCGAGCCAGGACTGCTCTTGAAGGGAGACATCTTGCTGAAGTGCTACCACAAGAAGTTCCGAAGCCCAGCCCGAGACGTCATCTTCCGTGTGCAGTTCCACACCTGTGCCATCCATGACCTGGGGGTTGTCTTTGGGAAGGAGGACCTTGATGATGCTTTCAAAGATGATCGATTTCCAGAGTATGGCAAAGTGGAGTTTGTATTTTCTTATGGGCCAGAGAAAATTCAAGGCATGGAGCACCTGGAGAACGGGCCGAGCGTGTCTGTGGACTATAACACCTCTGACCCCCTCATCCGCTGGGACTCCTACGACAACTTCAGTGGGCATCGAGATGACGGCATGGAGGAGGTGGTGGGACACACGCAGGGGCCACTAGATGGGAGCCTGTATGCTAAGGTGAAGAAGAAAGACTCCCTGCACGGCAGCACCGGGGCTGTTAATGCCACACGTCCTACACTGTCGGCCACCCCCAACCACGTGGAACACACGCTTTCTGTGAGCAGCGACTCGGGCAACTCCACAGCCTCCACCAAGACCGACAAGACCGACGAGCCTGTCCCCGGGGCCTCCAGTGCCACTGCTGCCTTGAGTCCCCAGGAGAAGCGGGAGCTGGACCGCCTGCTGAGTGGCTTTGGCTTAGAGCGAGAGAAGCAAGGCGCCATGTACCACACCCAGCACCTCAGGTCCCGCCCAGCAGGGGGCTCGGCTGTGCCCTCCTCTGGACGCCACGTTGTCCCAGCCCAGGTTCATGTCAATGGTGGGGCGTTAGCATCTGAGCGGGAGACAGACATCCTGGACGATGAATTGCCAAACCAGGATGGTCACAGTGCGGGCAGCATGGGCACACTCTCTTCTCTGGACGGGGTCACCAACACCAGTGAGGGGGGCTACCCAGAGGCCCTGTCCCCACTGACCAACGGTCTGGACAAGTCCTACCCCATGGAGCCTATGGTCAATGGAGGAGGCTACCCCTACGAGTCTGCCAGCCGGGCGGGGCCTGCCCATGCTGGCCACACGGCCCCCATGCGGCCCTCCTACTCTGCACAGGAGGGTTTAGCTGGCTACCAGAGGGAGGGGCCCCACCCAGCCTGGCCACAGCCAGTGACCACCTCCCACTATGCCCATGACCCCAGCGGTATGTTCCGCTCTCAATCCTTTTCGGAAGCTGAACCCCAGCTGCCCCCAGCTCCGGTCCGAGGGGGAAGCAGCCGGGAGGCTGTGCAAAGGGGACTGAATTCATGGCAGCAGCAGCAGCAGCAGCAGCAGCAGCCTCGCCCACCTCCACGCCAGCAGGAAAGAGCCCACTTGGAGAGTCTTGTAGCCAGCAGGCCCAGCCCTCAGCCATTGGCAGAGACCCCCATCCCCAGTCTCCCTGAGTTCCCGCGAGCAGCCTCCCAGCAGGAGATTGAACAGTCCATCGAAACACTCAATATGCTGATGCTGGACCTGGAGCCAGCCTCCGCTGCTGCCCCACTACACAAGTCCCAGAGTGTCCCCGGGGCCTGGCCAGGGGCTTCTCCACTCTCCTCCCAGCCCCTCTCTGGATCCTCCCGTCAGTCCCATCCACTGACCCAGTCCAGATCTGGCTATATCCCCAGTGGGCATTCGTTGGGAACCCCTGAGCCAGCCCCACGGGCCTCTCTGGAGTCTGTCCCTCCTGGCAGGTCTTACTCACCTTATGACTATCAGCCATGTTTGGCTGGGCCTAACCAGGATTTCCATTCAAAGAGCCCAGCCTCTTCCTCCTTGCCTGCCTTCCTTCCGACCACCCACAGCCCTCCAGGGCCTCAGCAACCCCCAGCCTCTCTCCCTGGCCTCACTGCTCAGCCTCTGCTCTCACCAAAGGAAGCGACTTCAGACCCCTCCCGGACTCCAGAGGAGGAGCCATTGAATTTAGAAGGGCTGGTGGCCCACAGGGTAGCAGGGGTACAGGCTCGGGAGAAGCAGCCTGCAGAGCCCCCAGCCCCTCTGCGGAGGCGGGCGGCCAGTGATGGACAGTATGAGAACCAGTCTCCAGAAGCCACATCCCCTCGTAGCCCTGGGGTTCGCTCCCCTGTCCAGTGTGTCTCCCCGGAGCTGGCTCTTACCATCGCTCTCAATCCTGGAGGGCGGCCCAAAGAGCCCCATTTGCACAGCTACAAGGAGGCCTTCGAGGAGATGGAGGGAACCTCCCCGAGCAGCCCACCACCCAGTGGGGTGCGGTCCCCCCCGGGTCTGGCCAAGACACCCCTGTCTGCTCTGGGCCTGAAACCTCACAACCCAGCGGACATCCTGTTGCACCCCACAGGAGTTACCAGAAGACGGATCCAGCCAGAGGAAGATGAGGGGAAGGTGGTGGTCAGGCTGTCAGAAGAGCCCCGGAGCTATGTGGAGTCTGTGGCACGGACAGCGGTGGCTGGACCCCGAGCTCAGGACTCTGAGCCCAAGAGCTTTAGTGCTCCAGCCACCCAGGCCTATGGCCATGAGATACCCCTGAGGAACGGGACCCTGGGTGGCTCCTTTGTCTCCCCCAGCCCCCTCTCCACCAGCAGCCCCATCCTCAGTGCTGACAGCACTTCAGTGGGGAGTTTCCCGTCGGGAGAGAGCAGTGACCAGGGTCCCCGGACGCCCACCCAGCCTCTGTTGGAGTCTGGCTTCCGCTCAGGCAGCCTGGGACAGCCCAGCCCGTCTGCCCAGAGAAACTACCAGAGCTCTTCTCCTCTCCCGACTGTGGGCAGTAGCTACAGCAGCCCCGACTACTCACTTCAGCATTTCAGCTCCTCTCCGGAAAGCCAGGCTCGAGCTCAGTTCAGTGTGGCTGGCGTCCACACGGTGCCTGGGAGCCCTCAGGCGCGCCACAGAACAGTGGGCACCAACACTCCCCCTAGTCCTGGCTTCGGCTGGCGGGCCATCAATCCCAGCATGGCTGCCCCCAGCAGTCCCAGTTTGAGCCATCACCAGATGATGGGTCCACCAGGCACTGGCTTCCATGGTAGCACTGTCTCCAGCCCCCAGAGCAGTGCAGCGACCACCCCGGGGAGCCCCAGCCTGTGTCGGCACCCAGCAGGGGTCTACCAGGTTTCTGGCCTCCACAACAAAGTGGCCACCACCCCGGGGAGTCCCAGCCTGGGCCGGCACCCTGGGGCTCACCAAGGCAACCTGGCCTCCGGTCTTCATAGCAATGCAATAGCCAGCCCTGGAAGCCCCAGCCTGGGCCGTCACCTCGGAGGGTCTGGATCTGTGGTTCCCGGCAGCCCCTGCTTGGACCGGCATGTGGCCTATGGCGGCTATTCTACCCCGGAGGATCGGAGACCCACACTGTCCCGGCAGAGCAGTGCCTCTGGCTACCAGGCTCCTTCCACGCCCTCCTTCCCTGTCTCCCCTGCCTACTACCCTGGCCTGAGCAGCCCTGCCACCTCCCCGTCACCAGACTCCGCAGCCTTCCGGCAAGGGAGCCCAACACCAGCCTTGCCAGAGAAGCGAAGGATGTCAGTGGGAGACCGGGCAGGCAGCCTCCCCAACTATGCCACCATCAATGGGAAGGTGTCTTCGCCTGTCGCCAGCGGCATGTCCAGTCCCAGCGGGGGCAGCACCGTCTCCTTCTCCCACACTCTGCCCGACTTCTCCAAGTACTCCATGCCAGACAACAGCCCGGAGACGCGGGCTAAAGTGAAGTTTGTCCAGGACACTTCTAAGTATTGGTACAAGCCTGAGATCTCCAGGGAGCAGGCCATCGCGCTCCTCAAGGACCAGGAGCCGGGGGCCTTCATCATCCGCGACAGTCACTCCTTCCGAGGCGCGTACGGGCTGGCCATGAAGGTGTCTTCGCCACCTCCAACCATCATGCAGCAGAATAAAAAAGGAGACATGACCCATGAGCTGGTCAGGCATTTTCTGATAGAGACTGGCCCCAGAGGAGTCAAGCTCAAGGGCTGCCCCAATGAGCCAAACTTCGGATCGCTGTCTGCCCTGGTCTACCAGCACTCCATCATCCCATTGGCCCTGCCTTGCAAGCTGGTCATTCCAAACCGAGACCCCACAGATGAATCGAAAGATAGCTCCGGCCCTGCCAACTCAACTGCAGACCTGCTGAAACAAGGGGCAGCCTGCAATGTGCTCTTCATCAACTCTGTGGACATGGAGTCACTCACTGGGCCACAGGCCATCTCTAAAGCCACATCTGAGACGTTGGCTGCAGACCCCACGCCAGCTGCCACCATCGTTCACTTCAAAGTCTCTGCCCAGGGAATCACTCTGACTGACAACCAGAGAAAGCTCTTTTTCAGACGCCACTACCCTCTCAACACTGTCACCTTCTGTGACCTGGATCCACAGGAAAGAAAGTGGATGAAAACAGAGGGTGGTGCCCCTGCTAAGCTCTTCGGCTTCGTGGCCCGGAAGCAGGGCAGCACCACGGACAACGCCTGCCACCTCTTTGCTGAGCTTGACCCCAACCAGCCGGCCTCTGCCATCGTCAACTTCGTCTCCAAGGTCATGCTGAATGCCGGCCAAAAGAGATGAACCCTGCCCCTTGCCCAGGGCCAGTGCCATGGGGAAGGGGCTTGTGGGGAGGGGACCCATGAATCCTGACCACTCTTGAACCCAGAAGGAGGACTTTGGGCCAATTTCGGAGGAGAGAAGAAAGTGCAACGTGGGGAGAGGGAAGTGAATTGCAGAGGGGAGGGGGAAAAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAAAGATGGAGGAGAAGAACTTGGATTCCCCTGGGTAGATGGAAACTGCAAAAACCCAAAGCCTCCAAAACTAACCAGGTCCACCTAACACCCCCTCCCTCCCCTAAGAAGATGGATGTCCTCAAAAGAGAAGGAACAAACCTCCTTGGGAATCCACATTTTTTGGGGGAATGGAAAAGCTCTGTCTCCCTAACTCAACTGCTTTGCAAGGGGAAATCAAGCTGGGAGAATCTTTTTCTGGCCACCTGTAGGGTAGGTTGTCAAACCAAACAGAGCCACCGTGGGACATCAAGTGGAAGAACTTGTTTGCTTGAAAGTATCTCAGACCCAAGGCACCTCAGGTCTCTTTGCTGTGCCTCCACTATATTGTCGTGTGGGTGTGTGTCTGCACCCACATCCTCACACATTGATCTAGATCTGCCTTTATCCACTCGAATTATAAACAGCTCGGCTTGTCCTTGTCCCATGTGTTTGTAGACACACATGCATACTGTCCAAAGATTAGGGTTGGTGGTGGCAGTGCAGCAGGGGAGGGACAAACAACCAAGCTATGGGTGACAGAGGCTCTCTCCTGGTGCCTGCACCTGCACTCTAGTGACCCTGGGTGCCGCCAGACCCTTCTCTTCTACAAAGACCCCAGCAGGAGTGGGAGGGTCTGCAATGGCATCGCCCTGTCCTGCCTTGGCCAGAAGCCTGGAGCTTTGGTTTGAGGAGGTAGAGATATGTGTATCCATAGGAAGAGATCTGTCAGAACAGGCAGCTGTTGAGCTCGGGGTGTCTTCCCCAAGGCATGTGGCTCAGCAGCAAGAAAGGCAAGTTGCTCCTGCTGGGGCCCTGGACTCTGCCTTAGCTCCCACCTCTCAGCCTTGTTATTGGGTTTCATGCCCCTGGACCAGCCTTATCTCAGACCTGCTTACCTGCATGATGCCTTTTTGGGGGCTGGGGATTGAGTCTTGCTGCTCTGCCCAGCCCTGTTCTATTCTGCAGGGTCCCTGTGTTGGAATTCTCCCTGGGGAACCTACTTTCTGCTCAGTGAGGCTCCGGCCAGAAACCTGGAGTCCTTATCCTCCCCTCTGTAAGTGTTTTAGGGTCTGGCTTTTGCAGGCACCCTCTGACCTCAGCAGAGCTCCTGGGCCTGCTGCCTGCACACCACATCGCCTACCTACAATGCCAAAGCCTCACTGTCACCCTTTCTGCCTTGGTTTCCCTAGCTGAGCCACGCTGCCCATGCAGCAGAGGGCAGAAGGCTTGCACTTGGGCCAAAGGGCCTAAGGTCCACTGGACAGTTGGGAAAACACCTGACCACCATTTAAGGACTCTAAGCCAGAATGGAAAATTCACCAGGACTCCATTCTTAAGCCTATGCGAGTCCCCTAGAGAGAGGCATTGTACTGATATATAAATATTATATAATATATACATGAGACATACTGACAGAATCTGTAAGCTAATAAAATGTAAGAAAAGGTTAAAAAAAGAAAAAAAAAAAA
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 85439, GC content failed 1463, low tm 21474, high tm 45522, high any compl 6, high end compl 28, high repeat similarity 37, long poly-x seq 80, ok 16829
PRIMER_RIGHT_EXPLAIN=considered 85348, GC content failed 1805, low tm 21834, high tm 44877, high any compl 1, high end compl 5, high repeat similarity 10, long poly-x seq 93, ok 16723
PRIMER_PAIR_EXPLAIN=considered 2207, unacceptable product size 2138, high any compl 2, high end compl 5, ok 62
PRIMER_PAIR_PENALTY=0.0572
PRIMER_LEFT_PENALTY=0.048411
PRIMER_RIGHT_PENALTY=0.008825
PRIMER_LEFT_SEQUENCE=CAGCCTTATCTCAGACCTGCTTA
PRIMER_RIGHT_SEQUENCE=AGGATAAGGACTCCAGGTTTCTG
PRIMER_LEFT=6670,23
PRIMER_RIGHT=6844,23
PRIMER_LEFT_TM=60.048
PRIMER_RIGHT_TM=60.009
PRIMER_LEFT_GC_PERCENT=47.826
PRIMER_RIGHT_GC_PERCENT=47.826
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_MISPRIMING_SCORE=11.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_MISPRIMING_SCORE=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_PAIR_MISPRIMING_SCORE=23.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_LEFT_END_STABILITY=7.5000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=175
PRIMER_PAIR_PENALTY_1=0.0824
PRIMER_LEFT_1_PENALTY=0.073619
PRIMER_RIGHT_1_PENALTY=0.008825
PRIMER_LEFT_1_SEQUENCE=TTATCTCAGACCTGCTTACCTGC
PRIMER_RIGHT_1_SEQUENCE=AGGATAAGGACTCCAGGTTTCTG
PRIMER_LEFT_1=6675,23
PRIMER_RIGHT_1=6844,23
PRIMER_LEFT_1_TM=59.926
PRIMER_RIGHT_1_TM=60.009
PRIMER_LEFT_1_GC_PERCENT=47.826
PRIMER_RIGHT_1_GC_PERCENT=47.826
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_MISPRIMING_SCORE=11.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_PAIR_1_MISPRIMING_SCORE=23.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=170
PRIMER_PAIR_PENALTY_2=0.0976
PRIMER_LEFT_2_PENALTY=0.088773
PRIMER_RIGHT_2_PENALTY=0.008825
PRIMER_LEFT_2_SEQUENCE=GAAATCAAGCTGGGAGAATCTTT
PRIMER_RIGHT_2_SEQUENCE=AGGATAAGGACTCCAGGTTTCTG
PRIMER_LEFT_2=5989,23
PRIMER_RIGHT_2=6844,23
PRIMER_LEFT_2_TM=60.089
PRIMER_RIGHT_2_TM=60.009
PRIMER_LEFT_2_GC_PERCENT=39.130
PRIMER_RIGHT_2_GC_PERCENT=47.826
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, L1MD2 3'-end of L1 repeat (subfamily L1MD2) - a consensus sequence
PRIMER_RIGHT_2_MISPRIMING_SCORE=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_PAIR_2_MISPRIMING_SCORE=21.00, L1MD2 3'-end of L1 repeat (subfamily L1MD2) - a consensus sequence
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=856
PRIMER_PAIR_PENALTY_3=0.1035
PRIMER_LEFT_3_PENALTY=0.002945
PRIMER_RIGHT_3_PENALTY=0.100593
PRIMER_LEFT_3_SEQUENCE=GAGATTGAACAGTCCATCGAAAC
PRIMER_RIGHT_3_SEQUENCE=GCTTCTGGAGACTGGTTCTCATA
PRIMER_LEFT_3=2472,23
PRIMER_RIGHT_3=3091,23
PRIMER_LEFT_3_TM=59.997
PRIMER_RIGHT_3_TM=59.899
PRIMER_LEFT_3_GC_PERCENT=43.478
PRIMER_RIGHT_3_GC_PERCENT=47.826
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_3_MISPRIMING_SCORE=24.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=5.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=620
PRIMER_PAIR_PENALTY_4=0.1107
PRIMER_LEFT_4_PENALTY=0.101873
PRIMER_RIGHT_4_PENALTY=0.008825
PRIMER_LEFT_4_SEQUENCE=TCTGCCTTTATCCACTCGAATTA
PRIMER_RIGHT_4_SEQUENCE=AGGATAAGGACTCCAGGTTTCTG
PRIMER_LEFT_4=6193,23
PRIMER_RIGHT_4=6844,23
PRIMER_LEFT_4_TM=60.102
PRIMER_RIGHT_4_TM=60.009
PRIMER_LEFT_4_GC_PERCENT=39.130
PRIMER_RIGHT_4_GC_PERCENT=47.826
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_PAIR_4_MISPRIMING_SCORE=21.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_LEFT_4_END_STABILITY=6.2000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=652
PRIMER_PAIR_PENALTY_5=0.1127
PRIMER_LEFT_5_PENALTY=0.048411
PRIMER_RIGHT_5_PENALTY=0.064245
PRIMER_LEFT_5_SEQUENCE=CAGCCTTATCTCAGACCTGCTTA
PRIMER_RIGHT_5_SEQUENCE=TCACTGAGCAGAAAGTAGGTTCC
PRIMER_LEFT_5=6670,23
PRIMER_RIGHT_5=6812,23
PRIMER_LEFT_5_TM=60.048
PRIMER_RIGHT_5_TM=59.936
PRIMER_LEFT_5_GC_PERCENT=47.826
PRIMER_RIGHT_5_GC_PERCENT=47.826
PRIMER_LEFT_5_SELF_ANY=5.00
PRIMER_RIGHT_5_SELF_ANY=5.00
PRIMER_LEFT_5_SELF_END=2.00
PRIMER_RIGHT_5_SELF_END=2.00
PRIMER_LEFT_5_MISPRIMING_SCORE=11.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_5_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_5_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_5_END_STABILITY=7.5000
PRIMER_RIGHT_5_END_STABILITY=7.9000
PRIMER_PAIR_5_COMPL_ANY=6.00
PRIMER_PAIR_5_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_5=143
PRIMER_PAIR_PENALTY_6=0.1372
PRIMER_LEFT_6_PENALTY=0.088773
PRIMER_RIGHT_6_PENALTY=0.048411
PRIMER_LEFT_6_SEQUENCE=GAAATCAAGCTGGGAGAATCTTT
PRIMER_RIGHT_6_SEQUENCE=TAAGCAGGTCTGAGATAAGGCTG
PRIMER_LEFT_6=5989,23
PRIMER_RIGHT_6=6692,23
PRIMER_LEFT_6_TM=60.089
PRIMER_RIGHT_6_TM=60.048
PRIMER_LEFT_6_GC_PERCENT=39.130
PRIMER_RIGHT_6_GC_PERCENT=47.826
PRIMER_LEFT_6_SELF_ANY=5.00
PRIMER_RIGHT_6_SELF_ANY=5.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=2.00
PRIMER_LEFT_6_MISPRIMING_SCORE=12.00, L1MD2 3'-end of L1 repeat (subfamily L1MD2) - a consensus sequence
PRIMER_RIGHT_6_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_6_MISPRIMING_SCORE=21.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_6_END_STABILITY=7.0000
PRIMER_RIGHT_6_END_STABILITY=9.7000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_6=704
PRIMER_PAIR_PENALTY_7=0.1451
PRIMER_LEFT_7_PENALTY=0.088773
PRIMER_RIGHT_7_PENALTY=0.056374
PRIMER_LEFT_7_SEQUENCE=GAAATCAAGCTGGGAGAATCTTT
PRIMER_RIGHT_7_SEQUENCE=TGTCTACAAACACATGGGACAAG
PRIMER_LEFT_7=5989,23
PRIMER_RIGHT_7=6256,23
PRIMER_LEFT_7_TM=60.089
PRIMER_RIGHT_7_TM=59.944
PRIMER_LEFT_7_GC_PERCENT=39.130
PRIMER_RIGHT_7_GC_PERCENT=43.478
PRIMER_LEFT_7_SELF_ANY=5.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_MISPRIMING_SCORE=12.00, L1MD2 3'-end of L1 repeat (subfamily L1MD2) - a consensus sequence
PRIMER_RIGHT_7_MISPRIMING_SCORE=12.00, MER11B a consensus
PRIMER_PAIR_7_MISPRIMING_SCORE=21.00, LTR12 LTR from human ERV9 endogenous retroviral sequence (HRES-1/1)
PRIMER_LEFT_7_END_STABILITY=7.0000
PRIMER_RIGHT_7_END_STABILITY=6.7000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_7=268
PRIMER_PAIR_PENALTY_8=0.1503
PRIMER_LEFT_8_PENALTY=0.101873
PRIMER_RIGHT_8_PENALTY=0.048411
PRIMER_LEFT_8_SEQUENCE=TCTGCCTTTATCCACTCGAATTA
PRIMER_RIGHT_8_SEQUENCE=TAAGCAGGTCTGAGATAAGGCTG
PRIMER_LEFT_8=6193,23
PRIMER_RIGHT_8=6692,23
PRIMER_LEFT_8_TM=60.102
PRIMER_RIGHT_8_TM=60.048
PRIMER_LEFT_8_GC_PERCENT=39.130
PRIMER_RIGHT_8_GC_PERCENT=47.826
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=5.00
PRIMER_LEFT_8_SELF_END=2.00
PRIMER_RIGHT_8_SELF_END=2.00
PRIMER_LEFT_8_MISPRIMING_SCORE=12.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_8_MISPRIMING_SCORE=23.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_8_END_STABILITY=6.2000
PRIMER_RIGHT_8_END_STABILITY=9.7000
PRIMER_PAIR_8_COMPL_ANY=6.00
PRIMER_PAIR_8_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_8=500
PRIMER_PAIR_PENALTY_9=0.1530
PRIMER_LEFT_9_PENALTY=0.088773
PRIMER_RIGHT_9_PENALTY=0.064245
PRIMER_LEFT_9_SEQUENCE=GAAATCAAGCTGGGAGAATCTTT
PRIMER_RIGHT_9_SEQUENCE=TCACTGAGCAGAAAGTAGGTTCC
PRIMER_LEFT_9=5989,23
PRIMER_RIGHT_9=6812,23
PRIMER_LEFT_9_TM=60.089
PRIMER_RIGHT_9_TM=59.936
PRIMER_LEFT_9_GC_PERCENT=39.130
PRIMER_RIGHT_9_GC_PERCENT=47.826
PRIMER_LEFT_9_SELF_ANY=5.00
PRIMER_RIGHT_9_SELF_ANY=5.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=2.00
PRIMER_LEFT_9_MISPRIMING_SCORE=12.00, L1MD2 3'-end of L1 repeat (subfamily L1MD2) - a consensus sequence
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_9_MISPRIMING_SCORE=21.00, LTR12 LTR from human ERV9 endogenous retroviral sequence (HRES-1/1)
PRIMER_LEFT_9_END_STABILITY=7.0000
PRIMER_RIGHT_9_END_STABILITY=7.9000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_9=824
PRIMER_PAIR_PENALTY_10=0.1569
PRIMER_LEFT_10_PENALTY=0.148090
PRIMER_RIGHT_10_PENALTY=0.008825
PRIMER_LEFT_10_SEQUENCE=AAGAGAAGGAACAAACCTCCTTG
PRIMER_RIGHT_10_SEQUENCE=AGGATAAGGACTCCAGGTTTCTG
PRIMER_LEFT_10=5902,23
PRIMER_RIGHT_10=6844,23
PRIMER_LEFT_10_TM=60.148
PRIMER_RIGHT_10_TM=60.009
PRIMER_LEFT_10_GC_PERCENT=43.478
PRIMER_RIGHT_10_GC_PERCENT=47.826
PRIMER_LEFT_10_SELF_ANY=5.00
PRIMER_RIGHT_10_SELF_ANY=5.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=2.00
PRIMER_LEFT_10_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_10_MISPRIMING_SCORE=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_PAIR_10_MISPRIMING_SCORE=22.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_10_END_STABILITY=8.5000
PRIMER_RIGHT_10_END_STABILITY=7.0000
PRIMER_PAIR_10_COMPL_ANY=6.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_10=943
PRIMER_PAIR_PENALTY_11=0.1611
PRIMER_LEFT_11_PENALTY=0.152257
PRIMER_RIGHT_11_PENALTY=0.008825
PRIMER_LEFT_11_SEQUENCE=AAGTGGAAGAACTTGTTTGCTTG
PRIMER_RIGHT_11_SEQUENCE=AGGATAAGGACTCCAGGTTTCTG
PRIMER_LEFT_11=6067,23
PRIMER_RIGHT_11=6844,23
PRIMER_LEFT_11_TM=59.848
PRIMER_RIGHT_11_TM=60.009
PRIMER_LEFT_11_GC_PERCENT=39.130
PRIMER_RIGHT_11_GC_PERCENT=47.826
PRIMER_LEFT_11_SELF_ANY=5.00
PRIMER_RIGHT_11_SELF_ANY=5.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=2.00
PRIMER_LEFT_11_MISPRIMING_SCORE=12.00, MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_PAIR_11_MISPRIMING_SCORE=22.00, MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_LEFT_11_END_STABILITY=8.5000
PRIMER_RIGHT_11_END_STABILITY=7.0000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_11=778
PRIMER_PAIR_PENALTY_12=0.1611
PRIMER_LEFT_12_PENALTY=0.025403
PRIMER_RIGHT_12_PENALTY=0.135712
PRIMER_LEFT_12_SEQUENCE=GAGTCCTTATCCTCCCCTCTGTA
PRIMER_RIGHT_12_SEQUENCE=TTAAATGGTGGTCAGGTGTTTTC
PRIMER_LEFT_12=6832,23
PRIMER_RIGHT_12=7089,23
PRIMER_LEFT_12_TM=59.975
PRIMER_RIGHT_12_TM=60.136
PRIMER_LEFT_12_GC_PERCENT=52.174
PRIMER_RIGHT_12_GC_PERCENT=39.130
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=2.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_12_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_12_MISPRIMING_SCORE=21.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_12_END_STABILITY=5.7000
PRIMER_RIGHT_12_END_STABILITY=7.3000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_12=258
PRIMER_PAIR_PENALTY_13=0.1618
PRIMER_LEFT_13_PENALTY=0.134874
PRIMER_RIGHT_13_PENALTY=0.026932
PRIMER_LEFT_13_SEQUENCE=GTCTTCATAGCAATGCAATAGCC
PRIMER_RIGHT_13_SEQUENCE=CTATCTTTCGATTCATCTGTGGG
PRIMER_LEFT_13=4219,23
PRIMER_RIGHT_13=5107,23
PRIMER_LEFT_13_TM=60.135
PRIMER_RIGHT_13_TM=59.973
PRIMER_LEFT_13_GC_PERCENT=43.478
PRIMER_RIGHT_13_GC_PERCENT=43.478
PRIMER_LEFT_13_SELF_ANY=7.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=3.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_13_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_13_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_13_END_STABILITY=8.7000
PRIMER_RIGHT_13_END_STABILITY=9.4000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_13=889
PRIMER_PAIR_PENALTY_14=0.1624
PRIMER_LEFT_14_PENALTY=0.088773
PRIMER_RIGHT_14_PENALTY=0.073619
PRIMER_LEFT_14_SEQUENCE=GAAATCAAGCTGGGAGAATCTTT
PRIMER_RIGHT_14_SEQUENCE=GCAGGTAAGCAGGTCTGAGATAA
PRIMER_LEFT_14=5989,23
PRIMER_RIGHT_14=6697,23
PRIMER_LEFT_14_TM=60.089
PRIMER_RIGHT_14_TM=59.926
PRIMER_LEFT_14_GC_PERCENT=39.130
PRIMER_RIGHT_14_GC_PERCENT=47.826
PRIMER_LEFT_14_SELF_ANY=5.00
PRIMER_RIGHT_14_SELF_ANY=5.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_MISPRIMING_SCORE=12.00, L1MD2 3'-end of L1 repeat (subfamily L1MD2) - a consensus sequence
PRIMER_RIGHT_14_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_14_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_14_END_STABILITY=7.0000
PRIMER_RIGHT_14_END_STABILITY=5.9000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_14=709
PRIMER_PAIR_PENALTY_15=0.1625
PRIMER_LEFT_15_PENALTY=0.153720
PRIMER_RIGHT_15_PENALTY=0.008825
PRIMER_LEFT_15_SEQUENCE=CACTCGAATTATAAACAGCTCGG
PRIMER_RIGHT_15_SEQUENCE=AGGATAAGGACTCCAGGTTTCTG
PRIMER_LEFT_15=6205,23
PRIMER_RIGHT_15=6844,23
PRIMER_LEFT_15_TM=60.154
PRIMER_RIGHT_15_TM=60.009
PRIMER_LEFT_15_GC_PERCENT=43.478
PRIMER_RIGHT_15_GC_PERCENT=47.826
PRIMER_LEFT_15_SELF_ANY=6.00
PRIMER_RIGHT_15_SELF_ANY=5.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=2.00
PRIMER_LEFT_15_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_15_MISPRIMING_SCORE=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_PAIR_15_MISPRIMING_SCORE=21.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_LEFT_15_END_STABILITY=9.9000
PRIMER_RIGHT_15_END_STABILITY=7.0000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_15=640
PRIMER_PAIR_PENALTY_16=0.1637
PRIMER_LEFT_16_PENALTY=0.154904
PRIMER_RIGHT_16_PENALTY=0.008825
PRIMER_LEFT_16_SEQUENCE=ACTGTCCAAAGATTAGGGTTGGT
PRIMER_RIGHT_16_SEQUENCE=AGGATAAGGACTCCAGGTTTCTG
PRIMER_LEFT_16=6266,23
PRIMER_RIGHT_16=6844,23
PRIMER_LEFT_16_TM=60.155
PRIMER_RIGHT_16_TM=60.009
PRIMER_LEFT_16_GC_PERCENT=43.478
PRIMER_RIGHT_16_GC_PERCENT=47.826
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=5.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=2.00
PRIMER_LEFT_16_MISPRIMING_SCORE=11.00, MER4C a consensus
PRIMER_RIGHT_16_MISPRIMING_SCORE=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_PAIR_16_MISPRIMING_SCORE=22.00, reverse MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_LEFT_16_END_STABILITY=8.2000
PRIMER_RIGHT_16_END_STABILITY=7.0000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_16=579
PRIMER_PAIR_PENALTY_17=0.1661
PRIMER_LEFT_17_PENALTY=0.101873
PRIMER_RIGHT_17_PENALTY=0.064245
PRIMER_LEFT_17_SEQUENCE=TCTGCCTTTATCCACTCGAATTA
PRIMER_RIGHT_17_SEQUENCE=TCACTGAGCAGAAAGTAGGTTCC
PRIMER_LEFT_17=6193,23
PRIMER_RIGHT_17=6812,23
PRIMER_LEFT_17_TM=60.102
PRIMER_RIGHT_17_TM=59.936
PRIMER_LEFT_17_GC_PERCENT=39.130
PRIMER_RIGHT_17_GC_PERCENT=47.826
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=5.00
PRIMER_LEFT_17_SELF_END=2.00
PRIMER_RIGHT_17_SELF_END=2.00
PRIMER_LEFT_17_MISPRIMING_SCORE=12.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_17_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_17_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_17_END_STABILITY=6.2000
PRIMER_RIGHT_17_END_STABILITY=7.9000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_17=620
PRIMER_PAIR_PENALTY_18=0.1725
PRIMER_LEFT_18_PENALTY=0.002945
PRIMER_RIGHT_18_PENALTY=0.169572
PRIMER_LEFT_18_SEQUENCE=GAGATTGAACAGTCCATCGAAAC
PRIMER_RIGHT_18_SEQUENCE=TCTTTGAATGGAAATCCTGGTTA
PRIMER_LEFT_18=2472,23
PRIMER_RIGHT_18=2799,23
PRIMER_LEFT_18_TM=59.997
PRIMER_RIGHT_18_TM=59.830
PRIMER_LEFT_18_GC_PERCENT=43.478
PRIMER_RIGHT_18_GC_PERCENT=34.783
PRIMER_LEFT_18_SELF_ANY=5.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=2.00
PRIMER_LEFT_18_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_18_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_18_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_18_END_STABILITY=6.7000
PRIMER_RIGHT_18_END_STABILITY=7.2000
PRIMER_PAIR_18_COMPL_ANY=6.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_18=328
PRIMER_PAIR_PENALTY_19=0.1755
PRIMER_LEFT_19_PENALTY=0.101873
PRIMER_RIGHT_19_PENALTY=0.073619
PRIMER_LEFT_19_SEQUENCE=TCTGCCTTTATCCACTCGAATTA
PRIMER_RIGHT_19_SEQUENCE=GCAGGTAAGCAGGTCTGAGATAA
PRIMER_LEFT_19=6193,23
PRIMER_RIGHT_19=6697,23
PRIMER_LEFT_19_TM=60.102
PRIMER_RIGHT_19_TM=59.926
PRIMER_LEFT_19_GC_PERCENT=39.130
PRIMER_RIGHT_19_GC_PERCENT=47.826
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=5.00
PRIMER_LEFT_19_SELF_END=2.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_MISPRIMING_SCORE=12.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_19_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_19_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_19_END_STABILITY=6.2000
PRIMER_RIGHT_19_END_STABILITY=5.9000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_19=505
=