PRIMER_SEQUENCE_ID=NM_032781
SEQUENCE=GCGCACGGAGCGCGGGACGGAGCGCCAGGCGGACGGACCGAAGGACGGAGGCACCGAAGGACGGACGCCCCCGCACACGCAGACGCACAGAGCTCGGCGCGGCCCCCGTCGCATACACACTGGCACAGACACAAGCAGGGACACACGCAGACACACGCACACTCGCGCGCGCATCCTCCCGCCAGCCTGCCCGCCTGCTCGCCGGCGCCCGGAGCCCGCTCTGGCCGCTTGCTTTTTGCTGAGAAAGCTTCCTGCCCTGGAAGATGGCACCCTTCCCCATCCAGACACCTTGGGAATGAATTATGAGGGAGCCAGGAGTGAGAGAGAGAACCACGCTGCTGATGACTCCGAGGGAGGGGCCCTGGACATGTGCTGCAGTGAGAGGCTACCGGGTCTCCCCCAGCCGATAGTGATGGAGGCACTGGACGAGGCTGAAGGGCTCCAGGACTCACAGAGAGAGATGCCGCCACCCCCTCCTCCCTCGCCGCCCTCAGATCCAGCTCAGAAGCCACCACCTCGAGGCGCTGGGAGCCACTCCCTCACTGTCAGGAGCAGCCTGTGCCTGTTCGCTGCCTCACAGTTCCTGCTTGCCTGTGGGGTGCTCCGGTTCAGCGGTTATGGCCACATCTGGTCACAGAACGCCACAAACCTCGTCTCCTCTTTGCTGACGCTCCTGAAACAGCTGGAACCCACGGCCTGGCTTGACTCTGGGACGTGGGGAGTCCCCAGTCTGCTGCTGGTCTTTCTGTCCGTGGGCCTGGTCCTCGTTACCACCCTGGTGTGGCACCTCCTGAGGACACCCGCAGAGCCACCCACCCCACTGCCCCCTGAGGACAGGCGCCAGTCAGTGAGCCGCCAGCCCTCCTTCACCTACTCAGAGTGGATGGAGGAGAAGATCGAGGATGACTTCCTGGACCTCGACCCGGTGCCCGAGACTCCTGTGTTTGATTGTGTGATGGACATCAAGCCTGAGGCTGACCCCACCTCACTCACCGTCAAGTCCATGGGTCTGCAGGAGAGGAGGGGTTCCAATGTCTCCCTGACCCTGGACATGTGCACTCCGGGCTGCAACGAGGAGGGCTTTGGCTATCTCATGTCCCCACGTGAGGAGTCCGCCCGCGAGTACCTGCTCAGCGCCTCCCGTGTCCTCCAAGCAGAAGAGCTTCATGAAAAGGCCCTGGACCCTTTCCTGCTGCAGGCGGAATTCTTTGAAATCCCCATGAACTTTGTGGATCCGAAAGAGTACGACATCCCTGGGCTGGTGCGGAAGAACCGGTACAAAACCATACTTCCCAACCCTCACAGCAGAGTGTGTCTGACCTCACCAGACCCTGACGACCCTCTGAGTTCCTACATCAATGCCAACTACATCCGGGGCTATGGTGGGGAGGAGAAGGTGTACATCGCCACTCAGGGACCCATCGTCAGCACGGTCGCCGACTTCTGGCGCATGGTGTGGCAGGAGCACACGCCCATCATTGTCATGATCACCAACATCGAGGAGATGAACGAGAAATGCACCGAGTATTGGCCGGAGGAGCAGGTGGCGTACGACGGTGTTGAGATCACTGTGCAGAAAGTCATTCACACGGAGGATTACCGGCTGCGACTCATCTCCCTCAAGAGTGGGACTGAGGAGCGAGGCCTGAAGCATTACTGGTTCACATCCTGGCCCGACCAGAAGACCCCAGACCGGGCCCCCCCACTCCTGCACCTGGTGCGGGAGGTGGAGGAGGCAGCCCAGCAGGAGGGGCCCCACTGTGCCCCCATCATCGTCCACTGCAGTGCAGGGATTGGGAGGACCGGCTGCTTCATTGCCACCAGCATCTGCTGCCAGCAGCTGCGGCAGGAGGGTGTAGTGGACATCCTGAAGACCACGTGCCAGCTCCGTCAGGACAGGGGCGGCATGATCCAGACATGCGAGCAGTACCAGTTTGTGCACCACGTCATGAGCCTCTACGAAAAGCAGCTGTCCCACCAGTCCCCAGAATGACTGCGCTTCTCCTACAAGGTTCTCTGGGCACTGCCCAGCCTGAGTCTCGGCCCTCACCCAGGGCCCTGCCTCGGGTCCTGGGCCTGCTCCCCGCTTCCTCCCCTTCAGTCAGCTCCCTCTGTCCTCTGTCAGCCTGGCCTGACCCCTACCCTCCAGCATTGCTCTTCCTACTGTACATATTGGGGAGTGGGGGGCAGGGTCGGGAAGGGACATGCCAGGCCAGGCCTGGGGCCCCGGGGCCTGACCCACACCACGCAGACCCCGGGCTCCAGTTTTTAACGATGGTTCCATCAATACCTGATCCAGAATGTTTCCGTGCTACACTTTGTGTCCTGCTGCAATGTGTTCTGTCTGTCCATCCATCTCTGCCCTCTGTACCGGACACTGTGTCTCCTCAGCCAGGAAGGGGTAATGAGCTCCAGCCCCTAAGCAACCGGACTTGCCTGCCTCGGCCTCACCCGCACTTCTCCCAAAAGGCAGATGACGGGGAGTTAGGCATGGGGAGCTCCAGAAGGTCACCAGAGAGCTTTCAGCTGAGGGAGAGTTCTCTAGGTTGGAGTGGGCATCACAGCCAGGGTGGCCTCTGGGTGTCAGATGCTCTCAGGAGGGTGCCCAGCCTGTGAGGCACTGGCAAGGTAGGGGGCAGATGGGGCATGGAGAACCCAGAGGATCTAGGCCCTGTTGGGGAGGGGAGGGGAGCTCAAGGTTTGGGTGGGGACTCAGCCCAGATCTACGTGAGACATTTTTCTGTGTCACTGTGGGAAAGCCTTCCCAGAAGTCTCACTGCGTGTGTTGAGAGCCCATCAGGAGGGCATGCATGACTCTTTGGCAACATGTATTATCTTGGAGCCACGTGTTTTTATTGCTGACTTTAAATATTTATCCCACGGCAGACAGAGACATTTGGTGTCTTTTTATAATTCGCTCGTGGTCATTGAATAGAGCAATAAACGGAGCATTTTGAGCAAAACT
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 34103, GC content failed 1303, low tm 7761, high tm 18464, high any compl 7, high end compl 51, high repeat similarity 80, long poly-x seq 43, ok 6394
PRIMER_RIGHT_EXPLAIN=considered 34116, GC content failed 1115, low tm 8242, high tm 17999, high any compl 3, high end compl 14, high repeat similarity 20, long poly-x seq 43, ok 6680
PRIMER_PAIR_EXPLAIN=considered 4208, unacceptable product size 4111, high end compl 18, ok 79
PRIMER_PAIR_PENALTY=0.0962
PRIMER_LEFT_PENALTY=0.007752
PRIMER_RIGHT_PENALTY=0.088469
PRIMER_LEFT_SEQUENCE=TACTGTACATATTGGGGAGTGGG
PRIMER_RIGHT_SEQUENCE=AAACACGTGGCTCCAAGATAATA
PRIMER_LEFT=2161,23
PRIMER_RIGHT=2851,23
PRIMER_LEFT_TM=60.008
PRIMER_RIGHT_TM=59.912
PRIMER_LEFT_GC_PERCENT=47.826
PRIMER_RIGHT_GC_PERCENT=39.130
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_MISPRIMING_SCORE=12.00, L1PB3 3'-end of L1 repeat (subfamily L1PB3) - a consensus sequence
PRIMER_RIGHT_MISPRIMING_SCORE=11.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=5.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=691
PRIMER_PAIR_PENALTY_1=0.1249
PRIMER_LEFT_1_PENALTY=0.007752
PRIMER_RIGHT_1_PENALTY=0.117102
PRIMER_LEFT_1_SEQUENCE=TACTGTACATATTGGGGAGTGGG
PRIMER_RIGHT_1_SEQUENCE=GCTCCAAGATAATACATGTTGCC
PRIMER_LEFT_1=2161,23
PRIMER_RIGHT_1=2842,23
PRIMER_LEFT_1_TM=60.008
PRIMER_RIGHT_1_TM=59.883
PRIMER_LEFT_1_GC_PERCENT=47.826
PRIMER_RIGHT_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_MISPRIMING_SCORE=12.00, L1PB3 3'-end of L1 repeat (subfamily L1PB3) - a consensus sequence
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, L1MA5 3'-end of L1 repeat (subfamily L1MA5) - a consensus sequence
PRIMER_PAIR_1_MISPRIMING_SCORE=23.00, reverse L1PB3 3'-end of L1 repeat (subfamily L1PB3) - a consensus sequence
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=10.0000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=682
PRIMER_PAIR_PENALTY_2=0.2002
PRIMER_LEFT_2_PENALTY=0.115144
PRIMER_RIGHT_2_PENALTY=0.085102
PRIMER_LEFT_2_SEQUENCE=AAAGTCATTCACACGGAGGATTA
PRIMER_RIGHT_2_SEQUENCE=GGTCTTCAGGATGTCCACTACAC
PRIMER_LEFT_2=1576,23
PRIMER_RIGHT_2=1875,23
PRIMER_LEFT_2_TM=59.885
PRIMER_RIGHT_2_TM=59.915
PRIMER_LEFT_2_GC_PERCENT=39.130
PRIMER_RIGHT_2_GC_PERCENT=52.174
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_MISPRIMING_SCORE=11.00, L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence
PRIMER_RIGHT_2_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_2_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_2_END_STABILITY=5.9000
PRIMER_RIGHT_2_END_STABILITY=5.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=300
PRIMER_PAIR_PENALTY_3=0.2002
PRIMER_LEFT_3_PENALTY=0.115144
PRIMER_RIGHT_3_PENALTY=0.085102
PRIMER_LEFT_3_SEQUENCE=AAAGTCATTCACACGGAGGATTA
PRIMER_RIGHT_3_SEQUENCE=GTGGTCTTCAGGATGTCCACTAC
PRIMER_LEFT_3=1576,23
PRIMER_RIGHT_3=1877,23
PRIMER_LEFT_3_TM=59.885
PRIMER_RIGHT_3_TM=59.915
PRIMER_LEFT_3_GC_PERCENT=39.130
PRIMER_RIGHT_3_GC_PERCENT=52.174
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_MISPRIMING_SCORE=11.00, L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_3_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_3_END_STABILITY=5.9000
PRIMER_RIGHT_3_END_STABILITY=5.1000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=302
PRIMER_PAIR_PENALTY_4=0.2425
PRIMER_LEFT_4_PENALTY=0.007752
PRIMER_RIGHT_4_PENALTY=0.234797
PRIMER_LEFT_4_SEQUENCE=TACTGTACATATTGGGGAGTGGG
PRIMER_RIGHT_4_SEQUENCE=TGATGGAACCATCGTTAAAAACT
PRIMER_LEFT_4=2161,23
PRIMER_RIGHT_4=2285,23
PRIMER_LEFT_4_TM=60.008
PRIMER_RIGHT_4_TM=59.765
PRIMER_LEFT_4_GC_PERCENT=47.826
PRIMER_RIGHT_4_GC_PERCENT=34.783
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, L1PB3 3'-end of L1 repeat (subfamily L1PB3) - a consensus sequence
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, L1MD1 3'-end of L1 repeat (subfamily L1MD1) - a consensus sequence
PRIMER_PAIR_4_MISPRIMING_SCORE=23.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=125
PRIMER_PAIR_PENALTY_5=0.2425
PRIMER_LEFT_5_PENALTY=0.007752
PRIMER_RIGHT_5_PENALTY=0.234797
PRIMER_LEFT_5_SEQUENCE=TACTGTACATATTGGGGAGTGGG
PRIMER_RIGHT_5_SEQUENCE=GATGGAACCATCGTTAAAAACTG
PRIMER_LEFT_5=2161,23
PRIMER_RIGHT_5=2284,23
PRIMER_LEFT_5_TM=60.008
PRIMER_RIGHT_5_TM=59.765
PRIMER_LEFT_5_GC_PERCENT=47.826
PRIMER_RIGHT_5_GC_PERCENT=39.130
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=8.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=2.00
PRIMER_LEFT_5_MISPRIMING_SCORE=12.00, L1PB3 3'-end of L1 repeat (subfamily L1PB3) - a consensus sequence
PRIMER_RIGHT_5_MISPRIMING_SCORE=11.00, L1MD1 3'-end of L1 repeat (subfamily L1MD1) - a consensus sequence
PRIMER_PAIR_5_MISPRIMING_SCORE=22.00, reverse MER6 Nonautonomous DNA transposon
PRIMER_LEFT_5_END_STABILITY=9.4000
PRIMER_RIGHT_5_END_STABILITY=6.7000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_5=124
PRIMER_PAIR_PENALTY_6=0.2537
PRIMER_LEFT_6_PENALTY=0.007752
PRIMER_RIGHT_6_PENALTY=0.245968
PRIMER_LEFT_6_SEQUENCE=TACTGTACATATTGGGGAGTGGG
PRIMER_RIGHT_6_SEQUENCE=TCCAACCTAGAGAACTCTCCCTC
PRIMER_LEFT_6=2161,23
PRIMER_RIGHT_6=2550,23
PRIMER_LEFT_6_TM=60.008
PRIMER_RIGHT_6_TM=60.246
PRIMER_LEFT_6_GC_PERCENT=47.826
PRIMER_RIGHT_6_GC_PERCENT=52.174
PRIMER_LEFT_6_SELF_ANY=6.00
PRIMER_RIGHT_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_MISPRIMING_SCORE=12.00, L1PB3 3'-end of L1 repeat (subfamily L1PB3) - a consensus sequence
PRIMER_RIGHT_6_MISPRIMING_SCORE=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_6_MISPRIMING_SCORE=20.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_6_END_STABILITY=9.4000
PRIMER_RIGHT_6_END_STABILITY=9.4000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_6=390
PRIMER_PAIR_PENALTY_7=0.2587
PRIMER_LEFT_7_PENALTY=0.170187
PRIMER_RIGHT_7_PENALTY=0.088469
PRIMER_LEFT_7_SEQUENCE=AGAGTTCTCTAGGTTGGAGTGGG
PRIMER_RIGHT_7_SEQUENCE=AAACACGTGGCTCCAAGATAATA
PRIMER_LEFT_7=2533,23
PRIMER_RIGHT_7=2851,23
PRIMER_LEFT_7_TM=60.170
PRIMER_RIGHT_7_TM=59.912
PRIMER_LEFT_7_GC_PERCENT=52.174
PRIMER_RIGHT_7_GC_PERCENT=39.130
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=3.00
PRIMER_LEFT_7_MISPRIMING_SCORE=12.00, L1MC2 3'-end of L1 repeat (subfamily L1MC2) - a consensus sequence
PRIMER_RIGHT_7_MISPRIMING_SCORE=11.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_7_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_7_END_STABILITY=9.4000
PRIMER_RIGHT_7_END_STABILITY=5.2000
PRIMER_PAIR_7_COMPL_ANY=6.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_7=319
PRIMER_PAIR_PENALTY_8=0.2778
PRIMER_LEFT_8_PENALTY=0.007752
PRIMER_RIGHT_8_PENALTY=0.270039
PRIMER_LEFT_8_SEQUENCE=TACTGTACATATTGGGGAGTGGG
PRIMER_RIGHT_8_SEQUENCE=CACGTGGCTCCAAGATAATACAT
PRIMER_LEFT_8=2161,23
PRIMER_RIGHT_8=2848,23
PRIMER_LEFT_8_TM=60.008
PRIMER_RIGHT_8_TM=60.270
PRIMER_LEFT_8_GC_PERCENT=47.826
PRIMER_RIGHT_8_GC_PERCENT=43.478
PRIMER_LEFT_8_SELF_ANY=6.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=2.00
PRIMER_LEFT_8_MISPRIMING_SCORE=12.00, L1PB3 3'-end of L1 repeat (subfamily L1PB3) - a consensus sequence
PRIMER_RIGHT_8_MISPRIMING_SCORE=11.00, L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_PAIR_8_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_8_END_STABILITY=9.4000
PRIMER_RIGHT_8_END_STABILITY=5.6000
PRIMER_PAIR_8_COMPL_ANY=6.00
PRIMER_PAIR_8_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_8=688
PRIMER_PAIR_PENALTY_9=0.2873
PRIMER_LEFT_9_PENALTY=0.170187
PRIMER_RIGHT_9_PENALTY=0.117102
PRIMER_LEFT_9_SEQUENCE=AGAGTTCTCTAGGTTGGAGTGGG
PRIMER_RIGHT_9_SEQUENCE=GCTCCAAGATAATACATGTTGCC
PRIMER_LEFT_9=2533,23
PRIMER_RIGHT_9=2842,23
PRIMER_LEFT_9_TM=60.170
PRIMER_RIGHT_9_TM=59.883
PRIMER_LEFT_9_GC_PERCENT=52.174
PRIMER_RIGHT_9_GC_PERCENT=43.478
PRIMER_LEFT_9_SELF_ANY=6.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_MISPRIMING_SCORE=12.00, L1MC2 3'-end of L1 repeat (subfamily L1MC2) - a consensus sequence
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, L1MA5 3'-end of L1 repeat (subfamily L1MA5) - a consensus sequence
PRIMER_PAIR_9_MISPRIMING_SCORE=20.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_9_END_STABILITY=9.4000
PRIMER_RIGHT_9_END_STABILITY=10.0000
PRIMER_PAIR_9_COMPL_ANY=6.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_9=310
PRIMER_PAIR_PENALTY_10=0.3499
PRIMER_LEFT_10_PENALTY=0.115144
PRIMER_RIGHT_10_PENALTY=0.234797
PRIMER_LEFT_10_SEQUENCE=AAAGTCATTCACACGGAGGATTA
PRIMER_RIGHT_10_SEQUENCE=GATGGAACCATCGTTAAAAACTG
PRIMER_LEFT_10=1576,23
PRIMER_RIGHT_10=2284,23
PRIMER_LEFT_10_TM=59.885
PRIMER_RIGHT_10_TM=59.765
PRIMER_LEFT_10_GC_PERCENT=39.130
PRIMER_RIGHT_10_GC_PERCENT=39.130
PRIMER_LEFT_10_SELF_ANY=3.00
PRIMER_RIGHT_10_SELF_ANY=8.00
PRIMER_LEFT_10_SELF_END=2.00
PRIMER_RIGHT_10_SELF_END=2.00
PRIMER_LEFT_10_MISPRIMING_SCORE=11.00, L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence
PRIMER_RIGHT_10_MISPRIMING_SCORE=11.00, L1MD1 3'-end of L1 repeat (subfamily L1MD1) - a consensus sequence
PRIMER_PAIR_10_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_10_END_STABILITY=5.9000
PRIMER_RIGHT_10_END_STABILITY=6.7000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_10=709
PRIMER_PAIR_PENALTY_11=0.3499
PRIMER_LEFT_11_PENALTY=0.115144
PRIMER_RIGHT_11_PENALTY=0.234797
PRIMER_LEFT_11_SEQUENCE=AAAGTCATTCACACGGAGGATTA
PRIMER_RIGHT_11_SEQUENCE=TGATGGAACCATCGTTAAAAACT
PRIMER_LEFT_11=1576,23
PRIMER_RIGHT_11=2285,23
PRIMER_LEFT_11_TM=59.885
PRIMER_RIGHT_11_TM=59.765
PRIMER_LEFT_11_GC_PERCENT=39.130
PRIMER_RIGHT_11_GC_PERCENT=34.783
PRIMER_LEFT_11_SELF_ANY=3.00
PRIMER_RIGHT_11_SELF_ANY=8.00
PRIMER_LEFT_11_SELF_END=2.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_MISPRIMING_SCORE=11.00, L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, L1MD1 3'-end of L1 repeat (subfamily L1MD1) - a consensus sequence
PRIMER_PAIR_11_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_11_END_STABILITY=5.9000
PRIMER_RIGHT_11_END_STABILITY=6.7000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_11=710
PRIMER_PAIR_PENALTY_12=0.3611
PRIMER_LEFT_12_PENALTY=0.115144
PRIMER_RIGHT_12_PENALTY=0.245968
PRIMER_LEFT_12_SEQUENCE=AAAGTCATTCACACGGAGGATTA
PRIMER_RIGHT_12_SEQUENCE=TCCAACCTAGAGAACTCTCCCTC
PRIMER_LEFT_12=1576,23
PRIMER_RIGHT_12=2550,23
PRIMER_LEFT_12_TM=59.885
PRIMER_RIGHT_12_TM=60.246
PRIMER_LEFT_12_GC_PERCENT=39.130
PRIMER_RIGHT_12_GC_PERCENT=52.174
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=6.00
PRIMER_LEFT_12_SELF_END=2.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_MISPRIMING_SCORE=11.00, L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence
PRIMER_RIGHT_12_MISPRIMING_SCORE=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_12_MISPRIMING_SCORE=20.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_12_END_STABILITY=5.9000
PRIMER_RIGHT_12_END_STABILITY=9.4000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_12=975
PRIMER_PAIR_PENALTY_13=0.3631
PRIMER_LEFT_13_PENALTY=0.245968
PRIMER_RIGHT_13_PENALTY=0.117102
PRIMER_LEFT_13_SEQUENCE=GAGGGAGAGTTCTCTAGGTTGGA
PRIMER_RIGHT_13_SEQUENCE=GCTCCAAGATAATACATGTTGCC
PRIMER_LEFT_13=2528,23
PRIMER_RIGHT_13=2842,23
PRIMER_LEFT_13_TM=60.246
PRIMER_RIGHT_13_TM=59.883
PRIMER_LEFT_13_GC_PERCENT=52.174
PRIMER_RIGHT_13_GC_PERCENT=43.478
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=6.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_13_MISPRIMING_SCORE=12.00, L1MA5 3'-end of L1 repeat (subfamily L1MA5) - a consensus sequence
PRIMER_PAIR_13_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_13_END_STABILITY=8.5000
PRIMER_RIGHT_13_END_STABILITY=10.0000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_13=315
PRIMER_PAIR_PENALTY_14=0.3915
PRIMER_LEFT_14_PENALTY=0.007752
PRIMER_RIGHT_14_PENALTY=0.383728
PRIMER_LEFT_14_SEQUENCE=TACTGTACATATTGGGGAGTGGG
PRIMER_RIGHT_14_SEQUENCE=ATTGATGGAACCATCGTTAAAAA
PRIMER_LEFT_14=2161,23
PRIMER_RIGHT_14=2287,23
PRIMER_LEFT_14_TM=60.008
PRIMER_RIGHT_14_TM=59.616
PRIMER_LEFT_14_GC_PERCENT=47.826
PRIMER_RIGHT_14_GC_PERCENT=30.435
PRIMER_LEFT_14_SELF_ANY=6.00
PRIMER_RIGHT_14_SELF_ANY=8.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=2.00
PRIMER_LEFT_14_MISPRIMING_SCORE=12.00, L1PB3 3'-end of L1 repeat (subfamily L1PB3) - a consensus sequence
PRIMER_RIGHT_14_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_14_MISPRIMING_SCORE=23.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_14_END_STABILITY=9.4000
PRIMER_RIGHT_14_END_STABILITY=7.6000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_14=127
PRIMER_PAIR_PENALTY_15=0.4345
PRIMER_LEFT_15_PENALTY=0.349375
PRIMER_RIGHT_15_PENALTY=0.085102
PRIMER_LEFT_15_SEQUENCE=TCACTGTGCAGAAAGTCATTCAC
PRIMER_RIGHT_15_SEQUENCE=GGTCTTCAGGATGTCCACTACAC
PRIMER_LEFT_15=1565,23
PRIMER_RIGHT_15=1875,23
PRIMER_LEFT_15_TM=60.349
PRIMER_RIGHT_15_TM=59.915
PRIMER_LEFT_15_GC_PERCENT=43.478
PRIMER_RIGHT_15_GC_PERCENT=52.174
PRIMER_LEFT_15_SELF_ANY=5.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=3.00
PRIMER_RIGHT_15_SELF_END=2.00
PRIMER_LEFT_15_MISPRIMING_SCORE=12.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_15_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_15_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_15_END_STABILITY=6.7000
PRIMER_RIGHT_15_END_STABILITY=5.4000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_15=311
PRIMER_PAIR_PENALTY_16=0.4345
PRIMER_LEFT_16_PENALTY=0.349375
PRIMER_RIGHT_16_PENALTY=0.085102
PRIMER_LEFT_16_SEQUENCE=TCACTGTGCAGAAAGTCATTCAC
PRIMER_RIGHT_16_SEQUENCE=GTGGTCTTCAGGATGTCCACTAC
PRIMER_LEFT_16=1565,23
PRIMER_RIGHT_16=1877,23
PRIMER_LEFT_16_TM=60.349
PRIMER_RIGHT_16_TM=59.915
PRIMER_LEFT_16_GC_PERCENT=43.478
PRIMER_RIGHT_16_GC_PERCENT=52.174
PRIMER_LEFT_16_SELF_ANY=5.00
PRIMER_RIGHT_16_SELF_ANY=8.00
PRIMER_LEFT_16_SELF_END=3.00
PRIMER_RIGHT_16_SELF_END=2.00
PRIMER_LEFT_16_MISPRIMING_SCORE=12.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_16_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_16_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_16_END_STABILITY=6.7000
PRIMER_RIGHT_16_END_STABILITY=5.1000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_16=313
PRIMER_PAIR_PENALTY_17=0.4402
PRIMER_LEFT_17_PENALTY=0.170187
PRIMER_RIGHT_17_PENALTY=0.270039
PRIMER_LEFT_17_SEQUENCE=AGAGTTCTCTAGGTTGGAGTGGG
PRIMER_RIGHT_17_SEQUENCE=CACGTGGCTCCAAGATAATACAT
PRIMER_LEFT_17=2533,23
PRIMER_RIGHT_17=2848,23
PRIMER_LEFT_17_TM=60.170
PRIMER_RIGHT_17_TM=60.270
PRIMER_LEFT_17_GC_PERCENT=52.174
PRIMER_RIGHT_17_GC_PERCENT=43.478
PRIMER_LEFT_17_SELF_ANY=6.00
PRIMER_RIGHT_17_SELF_ANY=6.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=2.00
PRIMER_LEFT_17_MISPRIMING_SCORE=12.00, L1MC2 3'-end of L1 repeat (subfamily L1MC2) - a consensus sequence
PRIMER_RIGHT_17_MISPRIMING_SCORE=11.00, L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_PAIR_17_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_17_END_STABILITY=9.4000
PRIMER_RIGHT_17_END_STABILITY=5.6000
PRIMER_PAIR_17_COMPL_ANY=6.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_17=316
PRIMER_PAIR_PENALTY_18=0.4444
PRIMER_LEFT_18_PENALTY=0.007752
PRIMER_RIGHT_18_PENALTY=0.436656
PRIMER_LEFT_18_SEQUENCE=TACTGTACATATTGGGGAGTGGG
PRIMER_RIGHT_18_SEQUENCE=AACCTAGAGAACTCTCCCTCAGC
PRIMER_LEFT_18=2161,23
PRIMER_RIGHT_18=2547,23
PRIMER_LEFT_18_TM=60.008
PRIMER_RIGHT_18_TM=59.563
PRIMER_LEFT_18_GC_PERCENT=47.826
PRIMER_RIGHT_18_GC_PERCENT=52.174
PRIMER_LEFT_18_SELF_ANY=6.00
PRIMER_RIGHT_18_SELF_ANY=6.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=2.00
PRIMER_LEFT_18_MISPRIMING_SCORE=12.00, L1PB3 3'-end of L1 repeat (subfamily L1PB3) - a consensus sequence
PRIMER_RIGHT_18_MISPRIMING_SCORE=11.00, TAR1 Human telomere associated repeat sequence, complete sequence
PRIMER_PAIR_18_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_18_END_STABILITY=9.4000
PRIMER_RIGHT_18_END_STABILITY=8.2000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_18=387
PRIMER_PAIR_PENALTY_19=0.4611
PRIMER_LEFT_19_PENALTY=0.376018
PRIMER_RIGHT_19_PENALTY=0.085102
PRIMER_LEFT_19_SEQUENCE=AACCGGTACAAAACCATACTTCC
PRIMER_RIGHT_19_SEQUENCE=GGTCTTCAGGATGTCCACTACAC
PRIMER_LEFT_19=1270,23
PRIMER_RIGHT_19=1875,23
PRIMER_LEFT_19_TM=60.376
PRIMER_RIGHT_19_TM=59.915
PRIMER_LEFT_19_GC_PERCENT=43.478
PRIMER_RIGHT_19_GC_PERCENT=52.174
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=2.00
PRIMER_LEFT_19_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_19_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_19_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_19_END_STABILITY=8.2000
PRIMER_RIGHT_19_END_STABILITY=5.4000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_19=606
=