PRIMER_SEQUENCE_ID=NM_033332
SEQUENCE=CACGGAACAGCCCTCCTGGGGTCCCCACGAGCCGCGTCCTGCTGTGCCCCGGCGCCTACGCAGCAGCGGCCGCGGCCGCGGTGGGCACGCACGGTTACCCCGGGCAGCTCCGGCCGCCAGCTGCAGCCCCGTCGCCTCGGCCGCGCCAGCCGGCTGCGGGCACCTGGGGGCGGGCTGGGGGCGCCGGCCGCGGCAGGAGGCGCTGTAGCGAGGGCTGCGGCGCCGGTCCTGCGGCGGCCGCGGGAGGCAGCGGGGCAGGCGCTGTGGGCCGGGCTCCTCCTCCGGCTCCTGCGCGACCGCCTCCCGCCGGGCTCTGCCGGCGCCCGCCGTCCCCGCAGCGCCGCTCTGCGCCCGCCGCCCCGAGCGCCCGCGCGGGGCTGGCGGGAGCCTCGGCGGGCGCGCGGGCGCGCGGGGCCATGGTCGTGGCCCCCTGACGGGCCGCGGCCGCCTCCATGAAGCGGAAAAGCGAGCGGCGGTCGAGCTGGGCCGCCGCGCCCCCCTGCTCGCGGCGCTGCTCGTCGACCTCGCCGGGTGTGAAGAAGATCCGCAGCTCCACGCAGCAAGACCCGCGCCGCCGGGACCCCCAGGACGACGTGTACCTGGACATCACCGATCGCCTTTGTTTTGCCATTCTCTACAGCAGACCAAAGAGTGCATCAAATGTACATTATTTCAGCATAGATAATGAACTTGAATATGAGAACTTCTACGCAGATTTTGGACCACTCAATCTGGCAATGGTTTACAGATATTGTTGCAAGATCAATAAGAAATTAAAGTCCATTACAATGTTAAGGAAGAAAATTGTTCATTTTACTGGCTCTGATCAGAGAAAACAAGCAAATGCTGCCTTCCTTGTTGGATGCTACATGGTTATATATTTGGGGAGAACCCCAGAAGAAGCATATAGAATATTAATCTTTGGAGAGACATCCTATATTCCTTTCAGAGATGCTGCCTATGGAAGTTGCAATTTCTACATTACACTTCTTGACTGTTTTCATGCAGTAAAGAAGGCAATGCAGTATGGCTTCCTTAATTTCAACTCATTTAACCTTGATGAATATGAACACTATGAAAAAGCAGAAAATGGAGATTTAAATTGGATAATACCAGACCGATTTATTGCCTTCTGTGGACCTCATTCAAGAGCCAGACTTGAAAGTGGTTACCACCAACATTCTCCTGAGACTTATATTCAATATTTTAAGAATCACAATGTTACTACCATTATTCGTCTGAATAAAAGGATGTATGATGCCAAACGCTTTACGGATGCTGGCTTCGATCACCATGATCTTTTCTTTGCGGATGGCAGCACCCCTACTGATGCCATTGTCAAAGAATTCCTAGATATCTGTGAAAATGCTGAGGGTGCCATTGCAGTACATTGCAAAGCTGGCCTTGGTCGCACGGGCACTCTGATAGCCTGCTACATCATGAAGCATTACAGGATGACAGCAGCCGAGACCATTGCGTGGGTCAGGATCTGCAGACCTGGCTCGGTGATTGGGCCTCAGCAGCAGTTTTTGGTGATGAAGCAAACCAACCTCTGGCTGGAAGGGGACTATTTTCGTCAGAAGTTAAAGGGGCAGGAGAATGGACAACACAGAGCAGCCTTCTCCAAACTTCTCTCTGGCGTTGATGACATTTCCATAAATGGGGTCGAGAATCAAGATCAGCAAGAACCCGAACCGTACAGTGATGATGACGAAATCAATGGAGTGACACAAGGTGATAGACTTCGGGCCTTGAAAAGCAGAAGACAATCCAAAACAAACGCTATTCCTCTCACCGATGGTTGGCTGTCCCAGGCTGTCACCTTTCTAGACCGGCTTCTGATCTGGCTCGGGATCCACAAGGACTAGACCTGCGGGGAAGGTCTCTCCTGGACACGCCCGTTGCCCACTGCAAGTTCTCTCCAGGTGCAATTGAAGCCTCTCAGCAGCGGAGGCCGCCATGTGGAGAGAGCAGGCAGGCCCACTGCTGCTGAGAACAGGGCAGGCACGGGCAGCTCCTGTTCTGCCTTTCCCAGCTTCGGAGACGCAGGCTCAGCTGCTCCGAAGCACCTGCCAGCACCGCACAGTACAGTTTCAGAGGACAGCAGTCTCCTTCCCGTGAAGCTCCCATGTGCTGGAATGGCATGGACTTGCTGATCAACAGAAGGAAATGGTCTGAAGTCTGACCAGCACAAGGAAGGAGGCTGGCTGGCTCAGAGGGGCCCACCTTGCGTGGAATGAAAACGCCAAAGGCTCATGAGCAACATTAGGCTAGAGGGGTCTTGTTCAAAGCATCCAACTCTGACTTCGGAGGCATTCCCAGCCGGCAGCAGTGTGTCCAGCCTGCCTCTTCCCAGGCTGGTCTGACATGCAGCTTAGGCTTTCATCCCAAGTTAGGTACTGACCCCTCCCTCTTGGGCAGCACCTCCCTTTTTAAAAAAATTTTTTTTTCTTCCAAAGACAGAGTCTTGCTCTTGTTGTCCAGGCTGGAGTGCAGTGGCGCGATCTAGGCTCACTGCAACCTCCTTCTCCCAGGTTCAAGCGACTCTCCTGCCTCAGCCTCCTGAGTAGCTGGGATTATAGGCGTCTGCCACCACGCCCGGCTAATTTCTGTATTTTTAGTAGAGACAGGGTTTCACCATGTTGGCCAGGCTGGTCTCGAACTCCTGACCTCAAGTGATCTGCCTGCCTTGGCCTCCCAAAGTGCTGGGATTACAGGTGTCAGCCACCGCACCCAGCCAAGCACCCCTATCTCTAGAGGATCTGGCCCCCCAGCCCAGTTACTGCAGGGCAGCTTTCCCCACCTGGTGACAGGCTGTGCGCAGCAGCCCCAGGACCTCACCCTGAGCTGAGTCTTCAGGAGCCGCCCTGGTGGCACAACTCAGACACCCCTGAGGCCTAGCAGTCAACTCCTGATTCAGACATGATCCAGTCCAGCCTGGGCTTGGCTATAACCAGCTCAAACTTGCTTGACCTCCACTTTTCAGGAGACTTGGGGACGACAGCCCTCATCGGCGTCTTTCATGGGGTTAATCTGCTTGAGTCTAAGTCGCCAGCCAGAAACGTGGTGCCCAGGGTGCCCTGCCTCAGGACATGTCCACACCCACGTCACAAGCACCTGAGGAGTCCGGCCGGGGCACTGTGGTCCAAAAGGTCCTGCCGCCTCCGCATCTGACTGTCCCAACGGCATGCTGGTGACACCCCCCTGCCCTTCGCTTCTGTCCTCCCTGGCTTCTCTGGGGCACTTGGGGCTATGTACAACCTGGCACGATCCAGAAAGGGTGCAAACAAAATGCCTACATCCAGGCACACGACCAAGTCAGCGAGAGCTAGCCCTGGTAAGCAAACATAGCCCATTACAGGTTCAGAACGTGCACCGGGTTCCCCAAAACTGTCTTCAACCACATGACTCAACAGCTCTATGGGATAGGAACTGTCAGTGTTTTTGCAACTGCAACATTAAACCAAGTGCTGTGGGCTTTTCAAGTATTATTCACAACACTAAAGGAAAGTTTCTTCAAAGGGCTCTCTGGCTAATCTTCAAAGCCGCAGTTAGGCAAAATGACAGTGTGACAGCTTCAAAGCCACTGACTCATGACACAGCCCTGATGTTGTACCGGCTAGGTTCAGATTTCAGAAATCAGGGCACTTGCATCCATTGCCTTTTCCAGGAAAGGGAAGAAAACACTCAGTTGATAAACCTTAGTACTCAGATAATAAATAAGAGACCAAAAGTAGGCTATCACCCAAAGCAAACATCCTTAACTGACCCTAACGTGTATGGATTCAACTTTGATTATTCAACAAAATCATGACCGACTGCTGTGGCCTGGAGTAACCAAAGGACTGTTTTCTCTACACAAAGTCAGGAGCGAATACCAACCTTTATTTGCACTTGGGTTCCAGTTCAAAGCCACCTTAGACAGTGTGGCAAAGTGGGAAAAAGCACAGATCCTGGGACCAAGGTTCAGATTCCATCTCAAGCGAGCATATGAACTGTGTGACAACAGGCAGACAGTACCTCTGTGTCTATGAGAAAGCGGGGAGAGCAACACCCCAGCTTCTAGCAGCTCTACAGCTGCCTGGACCTGCAGGCCCTCCTAGGCCCACTTCCTCCCCAGCACAGTGTGTGTTCCCGGGCGTGTGTGGCTCTGGGTCCAGCTCTGTTCAGGGTGGGACTCCAGGTGAATTACTGAACCTCTGAGGTGTACCCCCAACCCCAAACTTTCACCAAAAGCAATAAAGAGGAACTCTAGAACTGGAGCCAGGACTAAGTGAGAAAAACTGCTTATAAGTGCTTAATAAATACTAGTTATTTACAACTTTTGCTCAAGCCGAGGGCAGAGGCCTTTGTACGCAGCTGCCGAACTCTGACTCTAGTTCTGCGGAAGAAAAGGATGCGGTATTTGCTTTTGCCATGATCCCTTTCCATTTGATTGGCAGGTTAAATAACATGGTTTTTGAAGTCACATACTTAATATTCTTCCTAAAAACCACCCAAACACTAGATGTGTGTGTGCACACACACAGAAACCACGAGGTAGTTTAAATCACCATTAAAAATCAACGCTTTCTCTGATTCTGTGTCACAGAGTGGTGGCCAGTGGCTACACAATAGCGATTTTTAAATGATTGGTTAAGTGAAAACCAGAACTCAAAATATTCCAGGAGAGAAGATAACATTTACAAGTAAACAGTAAGTGCAATTGTATTTTAATTTCTTGGTCTCCGAAAACTCAGCTGTGACTGCTTTCCATTAACAGTTCCAGCTCTATGTGTTTCCTCTAACGCTAAAGGCACAGCCCCCGGGAATCTACTGCTTCCTAAGAGTCTCCATGGAGTCTATTTTACAACCTCCTTTCCCTCCATGCTTCCGCGGAGGAGTCTATACTATCTCTATATACACATTTTAAACATTATTCTTCATTTGAAATTCCTTCAATAAAAACACAGTCACCATTAAAAAAAA
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 57940, GC content failed 5033, low tm 21633, high tm 20022, high any compl 6, high end compl 49, high repeat similarity 45, long poly-x seq 68, ok 11084
PRIMER_RIGHT_EXPLAIN=considered 57936, GC content failed 4913, low tm 22203, high tm 19519, high end compl 11, high repeat similarity 2, long poly-x seq 79, ok 11209
PRIMER_PAIR_EXPLAIN=considered 995, unacceptable product size 919, high end compl 14, ok 62
PRIMER_PAIR_PENALTY=0.0405
PRIMER_LEFT_PENALTY=0.009608
PRIMER_RIGHT_PENALTY=0.030877
PRIMER_LEFT_SEQUENCE=GATCAACAGAAGGAAATGGTCTG
PRIMER_RIGHT_SEQUENCE=AACAAGACCCCTCTAGCCTAATG
PRIMER_LEFT=2154,23
PRIMER_RIGHT=2285,23
PRIMER_LEFT_TM=59.990
PRIMER_RIGHT_TM=60.031
PRIMER_LEFT_GC_PERCENT=43.478
PRIMER_RIGHT_GC_PERCENT=47.826
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_MISPRIMING_SCORE=21.00, LOR1 Human low copy repetitive sequence - a consensus
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=6.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=132
PRIMER_PAIR_PENALTY_1=0.0405
PRIMER_LEFT_1_PENALTY=0.009608
PRIMER_RIGHT_1_PENALTY=0.030877
PRIMER_LEFT_1_SEQUENCE=GATCAACAGAAGGAAATGGTCTG
PRIMER_RIGHT_1_SEQUENCE=CAAGACCCCTCTAGCCTAATGTT
PRIMER_LEFT_1=2154,23
PRIMER_RIGHT_1=2283,23
PRIMER_LEFT_1_TM=59.990
PRIMER_RIGHT_1_TM=60.031
PRIMER_LEFT_1_GC_PERCENT=43.478
PRIMER_RIGHT_1_GC_PERCENT=47.826
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, reverse MER4C a consensus
PRIMER_PAIR_1_MISPRIMING_SCORE=21.00, L1MD1 3'-end of L1 repeat (subfamily L1MD1) - a consensus sequence
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=130
PRIMER_PAIR_PENALTY_2=0.0405
PRIMER_LEFT_2_PENALTY=0.009608
PRIMER_RIGHT_2_PENALTY=0.030877
PRIMER_LEFT_2_SEQUENCE=ATCAACAGAAGGAAATGGTCTGA
PRIMER_RIGHT_2_SEQUENCE=AACAAGACCCCTCTAGCCTAATG
PRIMER_LEFT_2=2155,23
PRIMER_RIGHT_2=2285,23
PRIMER_LEFT_2_TM=59.990
PRIMER_RIGHT_2_TM=60.031
PRIMER_LEFT_2_GC_PERCENT=39.130
PRIMER_RIGHT_2_GC_PERCENT=47.826
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, reverse MER39 a consensus
PRIMER_RIGHT_2_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_2_MISPRIMING_SCORE=21.00, PTR5 Human PTR5 mRNA for repetitive sequence
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=131
PRIMER_PAIR_PENALTY_3=0.0405
PRIMER_LEFT_3_PENALTY=0.009608
PRIMER_RIGHT_3_PENALTY=0.030877
PRIMER_LEFT_3_SEQUENCE=ATCAACAGAAGGAAATGGTCTGA
PRIMER_RIGHT_3_SEQUENCE=CAAGACCCCTCTAGCCTAATGTT
PRIMER_LEFT_3=2155,23
PRIMER_RIGHT_3=2283,23
PRIMER_LEFT_3_TM=59.990
PRIMER_RIGHT_3_TM=60.031
PRIMER_LEFT_3_GC_PERCENT=39.130
PRIMER_RIGHT_3_GC_PERCENT=47.826
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, reverse MER39 a consensus
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, reverse MER4C a consensus
PRIMER_PAIR_3_MISPRIMING_SCORE=22.00, MER34 a consensus
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=129
PRIMER_PAIR_PENALTY_4=0.0423
PRIMER_LEFT_4_PENALTY=0.011391
PRIMER_RIGHT_4_PENALTY=0.030877
PRIMER_LEFT_4_SEQUENCE=AAGGGGACTATTTTCGTCAGAAG
PRIMER_RIGHT_4_SEQUENCE=AAGACCCCTCTAGCCTAATGTTG
PRIMER_LEFT_4=1560,23
PRIMER_RIGHT_4=2282,23
PRIMER_LEFT_4_TM=60.011
PRIMER_RIGHT_4_TM=60.031
PRIMER_LEFT_4_GC_PERCENT=43.478
PRIMER_RIGHT_4_GC_PERCENT=47.826
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_4_MISPRIMING_SCORE=11.00, MER28 Nonautonomous DNA transposon
PRIMER_PAIR_4_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=723
PRIMER_PAIR_PENALTY_5=0.0423
PRIMER_LEFT_5_PENALTY=0.011391
PRIMER_RIGHT_5_PENALTY=0.030877
PRIMER_LEFT_5_SEQUENCE=AAGGGGACTATTTTCGTCAGAAG
PRIMER_RIGHT_5_SEQUENCE=CAAGACCCCTCTAGCCTAATGTT
PRIMER_LEFT_5=1560,23
PRIMER_RIGHT_5=2283,23
PRIMER_LEFT_5_TM=60.011
PRIMER_RIGHT_5_TM=60.031
PRIMER_LEFT_5_GC_PERCENT=43.478
PRIMER_RIGHT_5_GC_PERCENT=47.826
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=5.00
PRIMER_LEFT_5_SELF_END=3.00
PRIMER_RIGHT_5_SELF_END=2.00
PRIMER_LEFT_5_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_5_MISPRIMING_SCORE=12.00, reverse MER4C a consensus
PRIMER_PAIR_5_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_5_END_STABILITY=6.7000
PRIMER_RIGHT_5_END_STABILITY=6.6000
PRIMER_PAIR_5_COMPL_ANY=6.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_5=724
PRIMER_PAIR_PENALTY_6=0.0423
PRIMER_LEFT_6_PENALTY=0.011391
PRIMER_RIGHT_6_PENALTY=0.030877
PRIMER_LEFT_6_SEQUENCE=AAGGGGACTATTTTCGTCAGAAG
PRIMER_RIGHT_6_SEQUENCE=AACAAGACCCCTCTAGCCTAATG
PRIMER_LEFT_6=1560,23
PRIMER_RIGHT_6=2285,23
PRIMER_LEFT_6_TM=60.011
PRIMER_RIGHT_6_TM=60.031
PRIMER_LEFT_6_GC_PERCENT=43.478
PRIMER_RIGHT_6_GC_PERCENT=47.826
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=5.00
PRIMER_LEFT_6_SELF_END=3.00
PRIMER_RIGHT_6_SELF_END=3.00
PRIMER_LEFT_6_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_6_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_6_MISPRIMING_SCORE=24.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_6_END_STABILITY=6.7000
PRIMER_RIGHT_6_END_STABILITY=6.2000
PRIMER_PAIR_6_COMPL_ANY=6.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_6=726
PRIMER_PAIR_PENALTY_7=0.0493
PRIMER_LEFT_7_PENALTY=0.038769
PRIMER_RIGHT_7_PENALTY=0.010529
PRIMER_LEFT_7_SEQUENCE=AAAAGTAGGCTATCACCCAAAGC
PRIMER_RIGHT_7_SEQUENCE=ACTACCTCGTGGTTTCTGTGTGT
PRIMER_LEFT_7=3729,23
PRIMER_RIGHT_7=4543,23
PRIMER_LEFT_7_TM=60.039
PRIMER_RIGHT_7_TM=60.011
PRIMER_LEFT_7_GC_PERCENT=43.478
PRIMER_RIGHT_7_GC_PERCENT=47.826
PRIMER_LEFT_7_SELF_ANY=5.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=2.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_7_MISPRIMING_SCORE=12.00, MER9 a consensus
PRIMER_PAIR_7_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_7_END_STABILITY=8.5000
PRIMER_RIGHT_7_END_STABILITY=6.4000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_7=815
PRIMER_PAIR_PENALTY_8=0.0508
PRIMER_LEFT_8_PENALTY=0.040223
PRIMER_RIGHT_8_PENALTY=0.010529
PRIMER_LEFT_8_SEQUENCE=CCAGGTGAATTACTGAACCTCTG
PRIMER_RIGHT_8_SEQUENCE=ACTACCTCGTGGTTTCTGTGTGT
PRIMER_LEFT_8=4179,23
PRIMER_RIGHT_8=4543,23
PRIMER_LEFT_8_TM=60.040
PRIMER_RIGHT_8_TM=60.011
PRIMER_LEFT_8_GC_PERCENT=47.826
PRIMER_RIGHT_8_GC_PERCENT=47.826
PRIMER_LEFT_8_SELF_ANY=6.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=2.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, MER9 a consensus
PRIMER_PAIR_8_MISPRIMING_SCORE=22.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_8_END_STABILITY=6.7000
PRIMER_RIGHT_8_END_STABILITY=6.4000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_8=365
PRIMER_PAIR_PENALTY_9=0.0509
PRIMER_LEFT_9_PENALTY=0.041315
PRIMER_RIGHT_9_PENALTY=0.009608
PRIMER_LEFT_9_SEQUENCE=TTCGATCACCATGATCTTTTCTT
PRIMER_RIGHT_9_SEQUENCE=TCAGACCATTTCCTTCTGTTGAT
PRIMER_LEFT_9=1283,23
PRIMER_RIGHT_9=2177,23
PRIMER_LEFT_9_TM=59.959
PRIMER_RIGHT_9_TM=59.990
PRIMER_LEFT_9_GC_PERCENT=34.783
PRIMER_RIGHT_9_GC_PERCENT=39.130
PRIMER_LEFT_9_SELF_ANY=7.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=2.00
PRIMER_LEFT_9_MISPRIMING_SCORE=12.00, MER4C a consensus
PRIMER_RIGHT_9_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_9_MISPRIMING_SCORE=22.00, reverse MER39 a consensus
PRIMER_LEFT_9_END_STABILITY=7.0000
PRIMER_RIGHT_9_END_STABILITY=6.9000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_9=895
PRIMER_PAIR_PENALTY_10=0.0527
PRIMER_LEFT_10_PENALTY=0.041315
PRIMER_RIGHT_10_PENALTY=0.011391
PRIMER_LEFT_10_SEQUENCE=TTCGATCACCATGATCTTTTCTT
PRIMER_RIGHT_10_SEQUENCE=CTTCTGACGAAAATAGTCCCCTT
PRIMER_LEFT_10=1283,23
PRIMER_RIGHT_10=1582,23
PRIMER_LEFT_10_TM=59.959
PRIMER_RIGHT_10_TM=60.011
PRIMER_LEFT_10_GC_PERCENT=34.783
PRIMER_RIGHT_10_GC_PERCENT=43.478
PRIMER_LEFT_10_SELF_ANY=7.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_MISPRIMING_SCORE=12.00, MER4C a consensus
PRIMER_RIGHT_10_MISPRIMING_SCORE=10.00, L1MA5 3'-end of L1 repeat (subfamily L1MA5) - a consensus sequence
PRIMER_PAIR_10_MISPRIMING_SCORE=21.00, reverse MER39 a consensus
PRIMER_LEFT_10_END_STABILITY=7.0000
PRIMER_RIGHT_10_END_STABILITY=9.7000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_10=300
PRIMER_PAIR_PENALTY_11=0.0566
PRIMER_LEFT_11_PENALTY=0.009608
PRIMER_RIGHT_11_PENALTY=0.046971
PRIMER_LEFT_11_SEQUENCE=GATCAACAGAAGGAAATGGTCTG
PRIMER_RIGHT_11_SEQUENCE=GCAAGACTCTGTCTTTGGAAGAA
PRIMER_LEFT_11=2154,23
PRIMER_RIGHT_11=2470,23
PRIMER_LEFT_11_TM=59.990
PRIMER_RIGHT_11_TM=60.047
PRIMER_LEFT_11_GC_PERCENT=43.478
PRIMER_RIGHT_11_GC_PERCENT=43.478
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=7.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=3.00
PRIMER_LEFT_11_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, MER41 Medium reiteration sequence MER41
PRIMER_PAIR_11_MISPRIMING_SCORE=21.00, L1MD1 3'-end of L1 repeat (subfamily L1MD1) - a consensus sequence
PRIMER_LEFT_11_END_STABILITY=6.4000
PRIMER_RIGHT_11_END_STABILITY=7.0000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_11=317
PRIMER_PAIR_PENALTY_12=0.0566
PRIMER_LEFT_12_PENALTY=0.009608
PRIMER_RIGHT_12_PENALTY=0.046971
PRIMER_LEFT_12_SEQUENCE=ATCAACAGAAGGAAATGGTCTGA
PRIMER_RIGHT_12_SEQUENCE=GCAAGACTCTGTCTTTGGAAGAA
PRIMER_LEFT_12=2155,23
PRIMER_RIGHT_12=2470,23
PRIMER_LEFT_12_TM=59.990
PRIMER_RIGHT_12_TM=60.047
PRIMER_LEFT_12_GC_PERCENT=39.130
PRIMER_RIGHT_12_GC_PERCENT=43.478
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=7.00
PRIMER_LEFT_12_SELF_END=3.00
PRIMER_RIGHT_12_SELF_END=3.00
PRIMER_LEFT_12_MISPRIMING_SCORE=12.00, reverse MER39 a consensus
PRIMER_RIGHT_12_MISPRIMING_SCORE=12.00, MER41 Medium reiteration sequence MER41
PRIMER_PAIR_12_MISPRIMING_SCORE=21.00, MER42a MER42 repetitive sequence: subfamily MER42a - a consensus
PRIMER_LEFT_12_END_STABILITY=6.7000
PRIMER_RIGHT_12_END_STABILITY=7.0000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_12=316
PRIMER_PAIR_PENALTY_13=0.0589
PRIMER_LEFT_13_PENALTY=0.010529
PRIMER_RIGHT_13_PENALTY=0.048411
PRIMER_LEFT_13_SEQUENCE=ACACACAGAAACCACGAGGTAGT
PRIMER_RIGHT_13_SEQUENCE=TGGAACTGTTAATGGAAAGCAGT
PRIMER_LEFT_13=4521,23
PRIMER_RIGHT_13=4766,23
PRIMER_LEFT_13_TM=60.011
PRIMER_RIGHT_13_TM=60.048
PRIMER_LEFT_13_GC_PERCENT=47.826
PRIMER_RIGHT_13_GC_PERCENT=39.130
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=5.00
PRIMER_LEFT_13_SELF_END=3.00
PRIMER_RIGHT_13_SELF_END=2.00
PRIMER_LEFT_13_MISPRIMING_SCORE=12.00, MER9 a consensus
PRIMER_RIGHT_13_MISPRIMING_SCORE=12.00, MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_PAIR_13_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_13_END_STABILITY=5.1000
PRIMER_RIGHT_13_END_STABILITY=7.9000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_13=246
PRIMER_PAIR_PENALTY_14=0.0652
PRIMER_LEFT_14_PENALTY=0.053769
PRIMER_RIGHT_14_PENALTY=0.011391
PRIMER_LEFT_14_SEQUENCE=AGACCAAAGAGTGCATCAAATGT
PRIMER_RIGHT_14_SEQUENCE=CTTCTGACGAAAATAGTCCCCTT
PRIMER_LEFT_14=641,23
PRIMER_RIGHT_14=1582,23
PRIMER_LEFT_14_TM=60.054
PRIMER_RIGHT_14_TM=60.011
PRIMER_LEFT_14_GC_PERCENT=39.130
PRIMER_RIGHT_14_GC_PERCENT=43.478
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=2.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_MISPRIMING_SCORE=11.00, L1MC2 3'-end of L1 repeat (subfamily L1MC2) - a consensus sequence
PRIMER_RIGHT_14_MISPRIMING_SCORE=10.00, L1MA5 3'-end of L1 repeat (subfamily L1MA5) - a consensus sequence
PRIMER_PAIR_14_MISPRIMING_SCORE=20.00, L1MA5 3'-end of L1 repeat (subfamily L1MA5) - a consensus sequence
PRIMER_LEFT_14_END_STABILITY=6.6000
PRIMER_RIGHT_14_END_STABILITY=9.7000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_14=942
PRIMER_PAIR_PENALTY_15=0.0760
PRIMER_LEFT_15_PENALTY=0.041315
PRIMER_RIGHT_15_PENALTY=0.034654
PRIMER_LEFT_15_SEQUENCE=TTCGATCACCATGATCTTTTCTT
PRIMER_RIGHT_15_SEQUENCE=GTTTGTTTTGGATTGTCTTCTGC
PRIMER_LEFT_15=1283,23
PRIMER_RIGHT_15=1780,23
PRIMER_LEFT_15_TM=59.959
PRIMER_RIGHT_15_TM=60.035
PRIMER_LEFT_15_GC_PERCENT=34.783
PRIMER_RIGHT_15_GC_PERCENT=39.130
PRIMER_LEFT_15_SELF_ANY=7.00
PRIMER_RIGHT_15_SELF_ANY=2.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=2.00
PRIMER_LEFT_15_MISPRIMING_SCORE=12.00, MER4C a consensus
PRIMER_RIGHT_15_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_15_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_15_END_STABILITY=7.0000
PRIMER_RIGHT_15_END_STABILITY=8.2000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_15=498
PRIMER_PAIR_PENALTY_16=0.0778
PRIMER_LEFT_16_PENALTY=0.030877
PRIMER_RIGHT_16_PENALTY=0.046971
PRIMER_LEFT_16_SEQUENCE=CAACATTAGGCTAGAGGGGTCTT
PRIMER_RIGHT_16_SEQUENCE=GCAAGACTCTGTCTTTGGAAGAA
PRIMER_LEFT_16=2260,23
PRIMER_RIGHT_16=2470,23
PRIMER_LEFT_16_TM=60.031
PRIMER_RIGHT_16_TM=60.047
PRIMER_LEFT_16_GC_PERCENT=47.826
PRIMER_RIGHT_16_GC_PERCENT=43.478
PRIMER_LEFT_16_SELF_ANY=5.00
PRIMER_RIGHT_16_SELF_ANY=7.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=3.00
PRIMER_LEFT_16_MISPRIMING_SCORE=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_16_MISPRIMING_SCORE=12.00, MER41 Medium reiteration sequence MER41
PRIMER_PAIR_16_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_16_END_STABILITY=6.4000
PRIMER_RIGHT_16_END_STABILITY=7.0000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_16=211
PRIMER_PAIR_PENALTY_17=0.0778
PRIMER_LEFT_17_PENALTY=0.030877
PRIMER_RIGHT_17_PENALTY=0.046971
PRIMER_LEFT_17_SEQUENCE=CATTAGGCTAGAGGGGTCTTGTT
PRIMER_RIGHT_17_SEQUENCE=GCAAGACTCTGTCTTTGGAAGAA
PRIMER_LEFT_17=2263,23
PRIMER_RIGHT_17=2470,23
PRIMER_LEFT_17_TM=60.031
PRIMER_RIGHT_17_TM=60.047
PRIMER_LEFT_17_GC_PERCENT=47.826
PRIMER_RIGHT_17_GC_PERCENT=43.478
PRIMER_LEFT_17_SELF_ANY=5.00
PRIMER_RIGHT_17_SELF_ANY=7.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=3.00
PRIMER_LEFT_17_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_17_MISPRIMING_SCORE=12.00, MER41 Medium reiteration sequence MER41
PRIMER_PAIR_17_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_17_END_STABILITY=7.0000
PRIMER_RIGHT_17_END_STABILITY=7.0000
PRIMER_PAIR_17_COMPL_ANY=6.00
PRIMER_PAIR_17_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_17=208
PRIMER_PAIR_PENALTY_18=0.0778
PRIMER_LEFT_18_PENALTY=0.030877
PRIMER_RIGHT_18_PENALTY=0.046971
PRIMER_LEFT_18_SEQUENCE=AACATTAGGCTAGAGGGGTCTTG
PRIMER_RIGHT_18_SEQUENCE=GCAAGACTCTGTCTTTGGAAGAA
PRIMER_LEFT_18=2261,23
PRIMER_RIGHT_18=2470,23
PRIMER_LEFT_18_TM=60.031
PRIMER_RIGHT_18_TM=60.047
PRIMER_LEFT_18_GC_PERCENT=47.826
PRIMER_RIGHT_18_GC_PERCENT=43.478
PRIMER_LEFT_18_SELF_ANY=5.00
PRIMER_RIGHT_18_SELF_ANY=7.00
PRIMER_LEFT_18_SELF_END=2.00
PRIMER_RIGHT_18_SELF_END=3.00
PRIMER_LEFT_18_MISPRIMING_SCORE=12.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_18_MISPRIMING_SCORE=12.00, MER41 Medium reiteration sequence MER41
PRIMER_PAIR_18_MISPRIMING_SCORE=22.00, MER41 Medium reiteration sequence MER41
PRIMER_LEFT_18_END_STABILITY=7.0000
PRIMER_RIGHT_18_END_STABILITY=7.0000
PRIMER_PAIR_18_COMPL_ANY=6.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_18=210
PRIMER_PAIR_PENALTY_19=0.0790
PRIMER_LEFT_19_PENALTY=0.038769
PRIMER_RIGHT_19_PENALTY=0.040223
PRIMER_LEFT_19_SEQUENCE=AAAAGTAGGCTATCACCCAAAGC
PRIMER_RIGHT_19_SEQUENCE=CAGAGGTTCAGTAATTCACCTGG
PRIMER_LEFT_19=3729,23
PRIMER_RIGHT_19=4201,23
PRIMER_LEFT_19_TM=60.039
PRIMER_RIGHT_19_TM=60.040
PRIMER_LEFT_19_GC_PERCENT=43.478
PRIMER_RIGHT_19_GC_PERCENT=47.826
PRIMER_LEFT_19_SELF_ANY=5.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=2.00
PRIMER_RIGHT_19_SELF_END=3.00
PRIMER_LEFT_19_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_19_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_19_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_19_END_STABILITY=8.5000
PRIMER_RIGHT_19_END_STABILITY=9.7000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_19=473
=