PRIMER_SEQUENCE_ID=NM_080391
SEQUENCE=AGCGGGGCTGCGCGAAGTCATCGCTGTTCCAGACAGCGATGACTCGAGAGCGGTGGGGGTGGCGGCGCGATCGGCCGGGCTGTAACCGTCGTCTGTCCGGGAGCGGCTGGAGCGGCAGCGGCGGCCGGGCACGGCGCGAGGTGACGCCACAGGGCAGCGGCGGCAGCGGAGGCAGCGGCGGCAGCAGGAGACGCAGCGGCGGCCGCAGCAGCAGCAGCAAGACGGACTCGTGGAGACGCGCCGCCGCCGCCGCCGCCGGGCCGGGCCGGGTGTCGCGCGCCGAGGCTGGGGGGGAGTCGTCGCCGCCGCCGCCACCGCTACCGCCGCCGCCGCCGCCGCCGAGGTGACTGAGGAGAGAGGCGCCTCCTCGCTCCCGCCACCGCCGGACTTCAATGCCCAGTCCCCAGCTCGCCAGCGTTTTTCGTTGGAATATACGTTGCACATTTATGGCGATTCTGAGTGTGAGGGCAGACTTCTGCCAGGCTCAGCACAGCATTTTCGCTGACAAGTGAGCTTGGAGGTTCTATGTGCCATAATTAACATTGCCTTGAAGACTCCTGGACACCGAGACTGGCCTCAGAAATAGTTGGCTTTTTTTTTTTTTTAATTGCAAGCATATTTCTTTTAATGACTCCAGTAAAATTAAGCATCAAGTAAACAAGTGGAAAGTGACCTACACTTTTAACTTGTCTCACTAGTGCCTAAATGTAGTAAAGGCTGCTTAAGTTTTGTATGTAGTTGGATTTTTTGGAGTCCGAATATTTCCATCTGCAGAAATTGAGGCCCAAATTGAATTTGGATTCAAGTGGATTCTAAATACTTTGCTTATCTTGAAGAGAGAAGCTTCATAAGGAATAAACAAGTTGAATAGAGAAAACACTGATTGATAATAGGCATTTTAGTGGTCTTTTTAATGTTTTCTGCTGTGAAACATTTCAAGATTTATTGATTTTTTTTTTTCACTTTCCCCATCACACTCACACGCACGCTCACACTTTTTATTTGCCATAATGAACCGTCCAGCCCCTGTGGAGATCTCCTATGAGAACATGCGTTTTCTGATAACTCACAACCCTACCAATGCTACTCTCAACAAGTTCACAGAGGAACTTAAGAAGTATGGAGTGACGACTTTGGTTCGAGTTTGTGATGCTACATATGATAAAGCTCCAGTTGAAAAAGAAGGAATCCACGTTCTAGATTGGCCATTTGATGATGGAGCTCCACCCCCTAATCAGATAGTAGATGATTGGTTAAACCTGTTAAAAACCAAATTTCGTGAAGAGCCAGGTTGCTGTGTTGCAGTGCATTGTGTTGCAGGATTGGGAAGGGCACCTGTGCTGGTTGCACTTGCTTTGATTGAATGTGGAATGAAGTACGAAGATGCAGTTCAGTTTATAAGACAAAAAAGAAGGGGAGCGTTCAATTCCAAACAGCTGCTTTATTTGGAGAAATACCGACCTAAGATGCGATTACGCTTCAGAGATACCAATGGGCATTGCTGTGTTCAGTAGAAGGAAATGTAAACGAAGGCTGACTTGATTGTGCCATTTAGAGGGAACTCTTGGTACCTGGAAATGTGAATCTGGAATATTACCTGTGTCATCAAAGTAGTGATGGATTCAGTACTCCTCAACCACTCTCCTAATGATTGGAACAAAAGCAAACAAAAAAGAAATCTCTCTATAAAATGAATAAAATGTTTAAGAAAAGAGAAAGAGAAAAGGAATTAATTCAGTGAAGGATGATTTTGCTCCTAGTTTTGGAGTTTGAATTTCTGCCAGGATTGAATTATTTTGAAATCTCCTGTCTTTTTAAACTTTTTCAAAATAGGTCTCTAAGGAAAACCAGCAGAACATTAGGCCTGTGCAAAACCATCTGTTTGGGGAGCACACTCTTGGCACATAGATCTCCCTGTGGTGGGATTTTTTTCCCTTTTTTTGTGGGGGAGGGTTGGTGGTATATTTTTCCCCTCTTTTTTCCTTCCTCTCCTACATCTCCCTTTTCCCCCGATCCAAGTTGTAGATGGAATAGAAGCCCTTGTTGCTGTAGATGTGCGTGCAGTCTGGCAGCCTTAAGCCCAC
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 22521, GC content failed 2748, low tm 10387, high tm 5042, high any compl 6, high end compl 28, high repeat similarity 22, long poly-x seq 164, ok 4124
PRIMER_RIGHT_EXPLAIN=considered 22510, GC content failed 2545, low tm 10611, high tm 4880, high end compl 3, high repeat similarity 8, long poly-x seq 169, ok 4294
PRIMER_PAIR_EXPLAIN=considered 299, unacceptable product size 240, high end compl 7, ok 52
PRIMER_PAIR_PENALTY=0.0666
PRIMER_LEFT_PENALTY=0.046971
PRIMER_RIGHT_PENALTY=0.019662
PRIMER_LEFT_SEQUENCE=GAAGTATGGAGTGACGACTTTGG
PRIMER_RIGHT_SEQUENCE=TCGGTATTTCTCCAAATAAAGCA
PRIMER_LEFT=1114,23
PRIMER_RIGHT=1459,23
PRIMER_LEFT_TM=60.047
PRIMER_RIGHT_TM=59.980
PRIMER_LEFT_GC_PERCENT=47.826
PRIMER_RIGHT_GC_PERCENT=34.783
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_MISPRIMING_SCORE=11.00, THE1BR THE-1B Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_RIGHT_MISPRIMING_SCORE=12.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_END_STABILITY=8.8000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=346
PRIMER_PAIR_PENALTY_1=0.0839
PRIMER_LEFT_1_PENALTY=0.064245
PRIMER_RIGHT_1_PENALTY=0.019662
PRIMER_LEFT_1_SEQUENCE=GACAAGTGAGCTTGGAGGTTCTA
PRIMER_RIGHT_1_SEQUENCE=TCGGTATTTCTCCAAATAAAGCA
PRIMER_LEFT_1=503,23
PRIMER_RIGHT_1=1459,23
PRIMER_LEFT_1_TM=59.936
PRIMER_RIGHT_1_TM=59.980
PRIMER_LEFT_1_GC_PERCENT=47.826
PRIMER_RIGHT_1_GC_PERCENT=34.783
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_MISPRIMING_SCORE=12.00, reverse 7SK Human family A3 repeated element DNA; 7SK RNA pseudogene
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_1_MISPRIMING_SCORE=21.00, Tigger1 Autonomous DNA transposon
PRIMER_LEFT_1_END_STABILITY=6.0000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=957
PRIMER_PAIR_PENALTY_2=0.0845
PRIMER_LEFT_2_PENALTY=0.046971
PRIMER_RIGHT_2_PENALTY=0.037505
PRIMER_LEFT_2_SEQUENCE=GAAGTATGGAGTGACGACTTTGG
PRIMER_RIGHT_2_SEQUENCE=AAGAGGGGAAAAATATACCACCA
PRIMER_LEFT_2=1114,23
PRIMER_RIGHT_2=1976,23
PRIMER_LEFT_2_TM=60.047
PRIMER_RIGHT_2_TM=59.962
PRIMER_LEFT_2_GC_PERCENT=47.826
PRIMER_RIGHT_2_GC_PERCENT=39.130
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_MISPRIMING_SCORE=11.00, THE1BR THE-1B Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_RIGHT_2_MISPRIMING_SCORE=12.00, MER42a MER42 repetitive sequence: subfamily MER42a - a consensus
PRIMER_PAIR_2_MISPRIMING_SCORE=21.00, MER42a MER42 repetitive sequence: subfamily MER42a - a consensus
PRIMER_LEFT_2_END_STABILITY=8.8000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=863
PRIMER_PAIR_PENALTY_3=0.0967
PRIMER_LEFT_3_PENALTY=0.059210
PRIMER_RIGHT_3_PENALTY=0.037505
PRIMER_LEFT_3_SEQUENCE=TAACTCACAACCCTACCAATGCT
PRIMER_RIGHT_3_SEQUENCE=AAGAGGGGAAAAATATACCACCA
PRIMER_LEFT_3=1062,23
PRIMER_RIGHT_3=1976,23
PRIMER_LEFT_3_TM=59.941
PRIMER_RIGHT_3_TM=59.962
PRIMER_LEFT_3_GC_PERCENT=43.478
PRIMER_RIGHT_3_GC_PERCENT=39.130
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_MISPRIMING_SCORE=11.00, LTR2 LTR from human endogenous retrovirus (4-14), 3' long terminal repeat
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, MER42a MER42 repetitive sequence: subfamily MER42a - a consensus
PRIMER_PAIR_3_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=915
PRIMER_PAIR_PENALTY_4=0.1112
PRIMER_LEFT_4_PENALTY=0.064245
PRIMER_RIGHT_4_PENALTY=0.046971
PRIMER_LEFT_4_SEQUENCE=GACAAGTGAGCTTGGAGGTTCTA
PRIMER_RIGHT_4_SEQUENCE=CCAAAGTCGTCACTCCATACTTC
PRIMER_LEFT_4=503,23
PRIMER_RIGHT_4=1136,23
PRIMER_LEFT_4_TM=59.936
PRIMER_RIGHT_4_TM=60.047
PRIMER_LEFT_4_GC_PERCENT=47.826
PRIMER_RIGHT_4_GC_PERCENT=47.826
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, reverse 7SK Human family A3 repeated element DNA; 7SK RNA pseudogene
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, Tigger2 Autonomous DNA transposon
PRIMER_PAIR_4_MISPRIMING_SCORE=21.00, Tigger2 Autonomous DNA transposon
PRIMER_LEFT_4_END_STABILITY=6.0000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=634
PRIMER_PAIR_PENALTY_5=0.1202
PRIMER_LEFT_5_PENALTY=0.082734
PRIMER_RIGHT_5_PENALTY=0.037505
PRIMER_LEFT_5_SEQUENCE=TTTTTATTTGCCATAATGAACCG
PRIMER_RIGHT_5_SEQUENCE=AAGAGGGGAAAAATATACCACCA
PRIMER_LEFT_5=995,23
PRIMER_RIGHT_5=1976,23
PRIMER_LEFT_5_TM=60.083
PRIMER_RIGHT_5_TM=59.962
PRIMER_LEFT_5_GC_PERCENT=30.435
PRIMER_RIGHT_5_GC_PERCENT=39.130
PRIMER_LEFT_5_SELF_ANY=7.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=2.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_5_MISPRIMING_SCORE=12.00, MER42a MER42 repetitive sequence: subfamily MER42a - a consensus
PRIMER_PAIR_5_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_5_END_STABILITY=9.9000
PRIMER_RIGHT_5_END_STABILITY=8.2000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_5=982
PRIMER_PAIR_PENALTY_6=0.1230
PRIMER_LEFT_6_PENALTY=0.085505
PRIMER_RIGHT_6_PENALTY=0.037505
PRIMER_LEFT_6_SEQUENCE=ACTCTTGGTACCTGGAAATGTGA
PRIMER_RIGHT_6_SEQUENCE=AAGAGGGGAAAAATATACCACCA
PRIMER_LEFT_6=1560,23
PRIMER_RIGHT_6=1976,23
PRIMER_LEFT_6_TM=59.914
PRIMER_RIGHT_6_TM=59.962
PRIMER_LEFT_6_GC_PERCENT=43.478
PRIMER_RIGHT_6_GC_PERCENT=39.130
PRIMER_LEFT_6_SELF_ANY=6.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_MISPRIMING_SCORE=11.00, MLT1c (MLT1c subfamily) - consensus sequence
PRIMER_RIGHT_6_MISPRIMING_SCORE=12.00, MER42a MER42 repetitive sequence: subfamily MER42a - a consensus
PRIMER_PAIR_6_MISPRIMING_SCORE=21.00, MER4C a consensus
PRIMER_LEFT_6_END_STABILITY=6.7000
PRIMER_RIGHT_6_END_STABILITY=8.2000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_6=417
PRIMER_PAIR_PENALTY_7=0.1235
PRIMER_LEFT_7_PENALTY=0.064245
PRIMER_RIGHT_7_PENALTY=0.059210
PRIMER_LEFT_7_SEQUENCE=GACAAGTGAGCTTGGAGGTTCTA
PRIMER_RIGHT_7_SEQUENCE=TAGCATTGGTAGGGTTGTGAGTT
PRIMER_LEFT_7=503,23
PRIMER_RIGHT_7=1085,23
PRIMER_LEFT_7_TM=59.936
PRIMER_RIGHT_7_TM=59.941
PRIMER_LEFT_7_GC_PERCENT=47.826
PRIMER_RIGHT_7_GC_PERCENT=43.478
PRIMER_LEFT_7_SELF_ANY=5.00
PRIMER_RIGHT_7_SELF_ANY=5.00
PRIMER_LEFT_7_SELF_END=2.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_MISPRIMING_SCORE=12.00, reverse 7SK Human family A3 repeated element DNA; 7SK RNA pseudogene
PRIMER_RIGHT_7_MISPRIMING_SCORE=11.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_7_MISPRIMING_SCORE=22.00, Tigger1 Autonomous DNA transposon
PRIMER_LEFT_7_END_STABILITY=6.0000
PRIMER_RIGHT_7_END_STABILITY=6.4000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_7=583
PRIMER_PAIR_PENALTY_8=0.1235
PRIMER_LEFT_8_PENALTY=0.064245
PRIMER_RIGHT_8_PENALTY=0.059210
PRIMER_LEFT_8_SEQUENCE=GACAAGTGAGCTTGGAGGTTCTA
PRIMER_RIGHT_8_SEQUENCE=AGCATTGGTAGGGTTGTGAGTTA
PRIMER_LEFT_8=503,23
PRIMER_RIGHT_8=1084,23
PRIMER_LEFT_8_TM=59.936
PRIMER_RIGHT_8_TM=59.941
PRIMER_LEFT_8_GC_PERCENT=47.826
PRIMER_RIGHT_8_GC_PERCENT=43.478
PRIMER_LEFT_8_SELF_ANY=5.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=2.00
PRIMER_RIGHT_8_SELF_END=2.00
PRIMER_LEFT_8_MISPRIMING_SCORE=12.00, reverse 7SK Human family A3 repeated element DNA; 7SK RNA pseudogene
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, reverse MER21 a consensus
PRIMER_PAIR_8_MISPRIMING_SCORE=21.00, Tigger1 Autonomous DNA transposon
PRIMER_LEFT_8_END_STABILITY=6.0000
PRIMER_RIGHT_8_END_STABILITY=5.7000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_8=582
PRIMER_PAIR_PENALTY_9=0.1262
PRIMER_LEFT_9_PENALTY=0.088722
PRIMER_RIGHT_9_PENALTY=0.037505
PRIMER_LEFT_9_SEQUENCE=ATTGGAACAAAAGCAAACAAAAA
PRIMER_RIGHT_9_SEQUENCE=AAGAGGGGAAAAATATACCACCA
PRIMER_LEFT_9=1650,23
PRIMER_RIGHT_9=1976,23
PRIMER_LEFT_9_TM=59.911
PRIMER_RIGHT_9_TM=59.962
PRIMER_LEFT_9_GC_PERCENT=26.087
PRIMER_RIGHT_9_GC_PERCENT=39.130
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, MER42a MER42 repetitive sequence: subfamily MER42a - a consensus
PRIMER_PAIR_9_MISPRIMING_SCORE=22.00, MER34 a consensus
PRIMER_LEFT_9_END_STABILITY=7.6000
PRIMER_RIGHT_9_END_STABILITY=8.2000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_9=327
PRIMER_PAIR_PENALTY_10=0.1263
PRIMER_LEFT_10_PENALTY=0.088826
PRIMER_RIGHT_10_PENALTY=0.037505
PRIMER_LEFT_10_SEQUENCE=TTTATTTGCCATAATGAACCGTC
PRIMER_RIGHT_10_SEQUENCE=AAGAGGGGAAAAATATACCACCA
PRIMER_LEFT_10=997,23
PRIMER_RIGHT_10=1976,23
PRIMER_LEFT_10_TM=60.089
PRIMER_RIGHT_10_TM=59.962
PRIMER_LEFT_10_GC_PERCENT=34.783
PRIMER_RIGHT_10_GC_PERCENT=39.130
PRIMER_LEFT_10_SELF_ANY=7.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_10_MISPRIMING_SCORE=12.00, MER42a MER42 repetitive sequence: subfamily MER42a - a consensus
PRIMER_PAIR_10_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_10_END_STABILITY=9.6000
PRIMER_RIGHT_10_END_STABILITY=8.2000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_10=980
PRIMER_PAIR_PENALTY_11=0.1297
PRIMER_LEFT_11_PENALTY=0.082734
PRIMER_RIGHT_11_PENALTY=0.046971
PRIMER_LEFT_11_SEQUENCE=TTTTTATTTGCCATAATGAACCG
PRIMER_RIGHT_11_SEQUENCE=CCAAAGTCGTCACTCCATACTTC
PRIMER_LEFT_11=995,23
PRIMER_RIGHT_11=1136,23
PRIMER_LEFT_11_TM=60.083
PRIMER_RIGHT_11_TM=60.047
PRIMER_LEFT_11_GC_PERCENT=30.435
PRIMER_RIGHT_11_GC_PERCENT=47.826
PRIMER_LEFT_11_SELF_ANY=7.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=2.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, Tigger2 Autonomous DNA transposon
PRIMER_PAIR_11_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_11_END_STABILITY=9.9000
PRIMER_RIGHT_11_END_STABILITY=6.4000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_11=142
PRIMER_PAIR_PENALTY_12=0.1358
PRIMER_LEFT_12_PENALTY=0.088826
PRIMER_RIGHT_12_PENALTY=0.046971
PRIMER_LEFT_12_SEQUENCE=TTTATTTGCCATAATGAACCGTC
PRIMER_RIGHT_12_SEQUENCE=CCAAAGTCGTCACTCCATACTTC
PRIMER_LEFT_12=997,23
PRIMER_RIGHT_12=1136,23
PRIMER_LEFT_12_TM=60.089
PRIMER_RIGHT_12_TM=60.047
PRIMER_LEFT_12_GC_PERCENT=34.783
PRIMER_RIGHT_12_GC_PERCENT=47.826
PRIMER_LEFT_12_SELF_ANY=7.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_12_MISPRIMING_SCORE=12.00, Tigger2 Autonomous DNA transposon
PRIMER_PAIR_12_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_12_END_STABILITY=9.6000
PRIMER_RIGHT_12_END_STABILITY=6.4000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_12=140
PRIMER_PAIR_PENALTY_13=0.1434
PRIMER_LEFT_13_PENALTY=0.064245
PRIMER_RIGHT_13_PENALTY=0.079168
PRIMER_LEFT_13_SEQUENCE=GACAAGTGAGCTTGGAGGTTCTA
PRIMER_RIGHT_13_SEQUENCE=GAACCAAAGTCGTCACTCCATAC
PRIMER_LEFT_13=503,23
PRIMER_RIGHT_13=1139,23
PRIMER_LEFT_13_TM=59.936
PRIMER_RIGHT_13_TM=59.921
PRIMER_LEFT_13_GC_PERCENT=47.826
PRIMER_RIGHT_13_GC_PERCENT=47.826
PRIMER_LEFT_13_SELF_ANY=5.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=2.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_MISPRIMING_SCORE=12.00, reverse 7SK Human family A3 repeated element DNA; 7SK RNA pseudogene
PRIMER_RIGHT_13_MISPRIMING_SCORE=12.00, reverse MER8 Nonautonomous DNA transposon
PRIMER_PAIR_13_MISPRIMING_SCORE=20.00, reverse MER21B a consensus
PRIMER_LEFT_13_END_STABILITY=6.0000
PRIMER_RIGHT_13_END_STABILITY=5.6000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_13=637
PRIMER_PAIR_PENALTY_14=0.1447
PRIMER_LEFT_14_PENALTY=0.059210
PRIMER_RIGHT_14_PENALTY=0.085505
PRIMER_LEFT_14_SEQUENCE=TAACTCACAACCCTACCAATGCT
PRIMER_RIGHT_14_SEQUENCE=TCACATTTCCAGGTACCAAGAGT
PRIMER_LEFT_14=1062,23
PRIMER_RIGHT_14=1582,23
PRIMER_LEFT_14_TM=59.941
PRIMER_RIGHT_14_TM=59.914
PRIMER_LEFT_14_GC_PERCENT=43.478
PRIMER_RIGHT_14_GC_PERCENT=43.478
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=3.00
PRIMER_RIGHT_14_SELF_END=2.00
PRIMER_LEFT_14_MISPRIMING_SCORE=11.00, LTR2 LTR from human endogenous retrovirus (4-14), 3' long terminal repeat
PRIMER_RIGHT_14_MISPRIMING_SCORE=11.00, MLT1c (MLT1c subfamily) - consensus sequence
PRIMER_PAIR_14_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_14_END_STABILITY=8.1000
PRIMER_RIGHT_14_END_STABILITY=6.1000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_14=521
PRIMER_PAIR_PENALTY_15=0.1470
PRIMER_LEFT_15_PENALTY=0.064245
PRIMER_RIGHT_15_PENALTY=0.082734
PRIMER_LEFT_15_SEQUENCE=GACAAGTGAGCTTGGAGGTTCTA
PRIMER_RIGHT_15_SEQUENCE=CGGTTCATTATGGCAAATAAAAA
PRIMER_LEFT_15=503,23
PRIMER_RIGHT_15=1017,23
PRIMER_LEFT_15_TM=59.936
PRIMER_RIGHT_15_TM=60.083
PRIMER_LEFT_15_GC_PERCENT=47.826
PRIMER_RIGHT_15_GC_PERCENT=30.435
PRIMER_LEFT_15_SELF_ANY=5.00
PRIMER_RIGHT_15_SELF_ANY=7.00
PRIMER_LEFT_15_SELF_END=2.00
PRIMER_RIGHT_15_SELF_END=3.00
PRIMER_LEFT_15_MISPRIMING_SCORE=12.00, reverse 7SK Human family A3 repeated element DNA; 7SK RNA pseudogene
PRIMER_RIGHT_15_MISPRIMING_SCORE=12.00, reverse L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_PAIR_15_MISPRIMING_SCORE=21.00, Tigger1 Autonomous DNA transposon
PRIMER_LEFT_15_END_STABILITY=6.0000
PRIMER_RIGHT_15_END_STABILITY=7.6000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_15=515
PRIMER_PAIR_PENALTY_16=0.1619
PRIMER_LEFT_16_PENALTY=0.082734
PRIMER_RIGHT_16_PENALTY=0.079168
PRIMER_LEFT_16_SEQUENCE=TTTTTATTTGCCATAATGAACCG
PRIMER_RIGHT_16_SEQUENCE=GAACCAAAGTCGTCACTCCATAC
PRIMER_LEFT_16=995,23
PRIMER_RIGHT_16=1139,23
PRIMER_LEFT_16_TM=60.083
PRIMER_RIGHT_16_TM=59.921
PRIMER_LEFT_16_GC_PERCENT=30.435
PRIMER_RIGHT_16_GC_PERCENT=47.826
PRIMER_LEFT_16_SELF_ANY=7.00
PRIMER_RIGHT_16_SELF_ANY=3.00
PRIMER_LEFT_16_SELF_END=2.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_16_MISPRIMING_SCORE=12.00, reverse MER8 Nonautonomous DNA transposon
PRIMER_PAIR_16_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_16_END_STABILITY=9.9000
PRIMER_RIGHT_16_END_STABILITY=5.6000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_16=145
PRIMER_PAIR_PENALTY_17=0.1680
PRIMER_LEFT_17_PENALTY=0.088826
PRIMER_RIGHT_17_PENALTY=0.079168
PRIMER_LEFT_17_SEQUENCE=TTTATTTGCCATAATGAACCGTC
PRIMER_RIGHT_17_SEQUENCE=GAACCAAAGTCGTCACTCCATAC
PRIMER_LEFT_17=997,23
PRIMER_RIGHT_17=1139,23
PRIMER_LEFT_17_TM=60.089
PRIMER_RIGHT_17_TM=59.921
PRIMER_LEFT_17_GC_PERCENT=34.783
PRIMER_RIGHT_17_GC_PERCENT=47.826
PRIMER_LEFT_17_SELF_ANY=7.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_17_MISPRIMING_SCORE=12.00, reverse MER8 Nonautonomous DNA transposon
PRIMER_PAIR_17_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_17_END_STABILITY=9.6000
PRIMER_RIGHT_17_END_STABILITY=5.6000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_17=143
PRIMER_PAIR_PENALTY_18=0.1682
PRIMER_LEFT_18_PENALTY=0.082734
PRIMER_RIGHT_18_PENALTY=0.085505
PRIMER_LEFT_18_SEQUENCE=TTTTTATTTGCCATAATGAACCG
PRIMER_RIGHT_18_SEQUENCE=TCACATTTCCAGGTACCAAGAGT
PRIMER_LEFT_18=995,23
PRIMER_RIGHT_18=1582,23
PRIMER_LEFT_18_TM=60.083
PRIMER_RIGHT_18_TM=59.914
PRIMER_LEFT_18_GC_PERCENT=30.435
PRIMER_RIGHT_18_GC_PERCENT=43.478
PRIMER_LEFT_18_SELF_ANY=7.00
PRIMER_RIGHT_18_SELF_ANY=6.00
PRIMER_LEFT_18_SELF_END=2.00
PRIMER_RIGHT_18_SELF_END=2.00
PRIMER_LEFT_18_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_18_MISPRIMING_SCORE=11.00, MLT1c (MLT1c subfamily) - consensus sequence
PRIMER_PAIR_18_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_18_END_STABILITY=9.9000
PRIMER_RIGHT_18_END_STABILITY=6.1000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_18=588
PRIMER_PAIR_PENALTY_19=0.1691
PRIMER_LEFT_19_PENALTY=0.046971
PRIMER_RIGHT_19_PENALTY=0.122120
PRIMER_LEFT_19_SEQUENCE=GAAGTATGGAGTGACGACTTTGG
PRIMER_RIGHT_19_SEQUENCE=TGGTTGAGGAGTACTGAATCCAT
PRIMER_LEFT_19=1114,23
PRIMER_RIGHT_19=1638,23
PRIMER_LEFT_19_TM=60.047
PRIMER_RIGHT_19_TM=59.878
PRIMER_LEFT_19_GC_PERCENT=47.826
PRIMER_RIGHT_19_GC_PERCENT=43.478
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=7.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=3.00
PRIMER_LEFT_19_MISPRIMING_SCORE=11.00, THE1BR THE-1B Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_RIGHT_19_MISPRIMING_SCORE=11.00, reverse L1MB7 3'-end of L1 repeat (subfamily L1MB7) - a consensus sequence
PRIMER_PAIR_19_MISPRIMING_SCORE=19.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_19_END_STABILITY=8.8000
PRIMER_RIGHT_19_END_STABILITY=8.1000
PRIMER_PAIR_19_COMPL_ANY=6.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_19=525
=