PRIMER_SEQUENCE_ID=NM_080611
SEQUENCE=CAACAAACTGCCGTTGTCCCGCGGGCCGTGGGCCCGGCACACTGGGGCTTTGTCCGGCCCCCCGCCGCGACCCGAGGCGGGGGCCCCACATGACCGAAGGGGTAGGAGGAGCCTGCGGCGGCGGCGGTGGCAGCGGCGGCTCCACCACCATTTCCCTCCCGGGCCGGGGGTCGGCGGGCGGTGGCGGTGGCTGGGCAGGGCTGGGCAGGGCCGCGGACGCCAGGCCCCCCGTTCCCCGCCAGGCTGCAGGCGTCGGGCCTGGGCCGTCAGGGCAGCTGTGACCGGATCGCTTCCCGGGCGGCGAGCTGGGGGTGCACCCGGACCGCCGCCCCCGGGATCATGGGCAATGGCATGACCAAGGTACTTCCTGGACTCTACCTCGGAAACTTCATTGATGCCAAAGACCTGGATCAGCTGGGCCGAAATAAGATCACACACATCATCTCTATCCATGAGTCACCCCAGCCTCTGCTGCAGGATATCACCTACCTTCGCATCCCGGTCGCTGATACCCCTGAGGTACCCATCAAAAAGCACTTCAAAGAATGTATCAACTTCATCCACTGCTGCCGCCTTAATGGGGGGAACTGCCTTGTGCACTGCTTTGCAGGCATCTCTCGCAGCACCACGATTGTGACAGCGTATGTGATGACTGTGACGGGGCTAGGCTGGCGGGACGTGCTTGAAGCCATCAAGGCCACCAGGCCCATCGCCAACCCCAACCCAGGCTTTAGGCAGCAGCTTGAAGAGTTTGGCTGGGCCAGTTCCCAGAAGCTTCGCCGGCAGCTGGAGGAGCGCTTCGGCGAGAGCCCCTTCCGCGACGAGGAGGAGTTGCGCGCGCTGCTGCCGCTGTGCAAGCGCTGCCGGCAGGGCTCCGCGACCTCGGCCTCCTCCGCCGGGCCGCACTCAGCAGCCTCCGAGGGAACCGTGCAGCGCCTGGTGCCGCGCACGCCCCGGGAAGCCCACCGGCCGCTGCCGCTGCTGGCGCGCGTCAAGCAGACTTTCTCTTGCCTCCCCCGGTGTCTGTCCCGCAAGGGCGGCAAGTGAGGATGCAGTCCAGCCGTGGCTCCCCACTTCCGACTGGCTCCCTTCGGGGGCTGTCTGCGCCTTCCACGCCCCCCAGGACGGGCCCAGAGGCTGGGGGAGCCCCGCGGCGGCCTGAACCCTGCCTCCCGCGCCCGCCCTGCTCGTCCGCGTCTGCAGTCAGCGTCCCCAACCTGTGCGTCTCTGTGTCCGGGCCGGCCTGCTGCAGCCACCTGGTGCCTTAGTCCTTGGGCTGGGGGAGGGGGCCCACCCTTAAAGGCGGCGGGAGGGGAGGGAGGGAGAGTGGAGGGTTTGACGGGCCTGGAGGGTATTAAAGAGACACAGAAGAAGCTGCCTGTC
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 15011, GC content failed 3321, low tm 1965, high tm 7842, high end compl 9, high repeat similarity 16, long poly-x seq 59, ok 1799
PRIMER_RIGHT_EXPLAIN=considered 15030, GC content failed 2862, low tm 2196, high tm 7963, high end compl 14, high repeat similarity 9, long poly-x seq 51, ok 1935
PRIMER_PAIR_EXPLAIN=considered 1983, unacceptable product size 1952, high end compl 2, ok 29
PRIMER_PAIR_PENALTY=0.1185
PRIMER_LEFT_PENALTY=0.000148
PRIMER_RIGHT_PENALTY=0.118384
PRIMER_LEFT_SEQUENCE=GAAACTTCATTGATGCCAAAGAC
PRIMER_RIGHT_SEQUENCE=GTCACAGTCATCACATACGCTGT
PRIMER_LEFT=382,23
PRIMER_RIGHT=658,23
PRIMER_LEFT_TM=60.000
PRIMER_RIGHT_TM=60.118
PRIMER_LEFT_GC_PERCENT=39.130
PRIMER_RIGHT_GC_PERCENT=47.826
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=277
PRIMER_PAIR_PENALTY_1=0.2434
PRIMER_LEFT_1_PENALTY=0.125045
PRIMER_RIGHT_1_PENALTY=0.118384
PRIMER_LEFT_1_SEQUENCE=CCGAAATAAGATCACACACATCA
PRIMER_RIGHT_1_SEQUENCE=GTCACAGTCATCACATACGCTGT
PRIMER_LEFT_1=419,23
PRIMER_RIGHT_1=658,23
PRIMER_LEFT_1_TM=59.875
PRIMER_RIGHT_1_TM=60.118
PRIMER_LEFT_1_GC_PERCENT=39.130
PRIMER_RIGHT_1_GC_PERCENT=47.826
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_1_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=240
PRIMER_PAIR_PENALTY_2=0.4606
PRIMER_LEFT_2_PENALTY=0.342192
PRIMER_RIGHT_2_PENALTY=0.118384
PRIMER_LEFT_2_SEQUENCE=TCATCTCTATCCATGAGTCACCC
PRIMER_RIGHT_2_SEQUENCE=GTCACAGTCATCACATACGCTGT
PRIMER_LEFT_2=439,23
PRIMER_RIGHT_2=658,23
PRIMER_LEFT_2_TM=60.342
PRIMER_RIGHT_2_TM=60.118
PRIMER_LEFT_2_GC_PERCENT=47.826
PRIMER_RIGHT_2_GC_PERCENT=47.826
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_2_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_2_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=220
PRIMER_PAIR_PENALTY_3=0.6111
PRIMER_LEFT_3_PENALTY=0.000148
PRIMER_RIGHT_3_PENALTY=0.610986
PRIMER_LEFT_3_SEQUENCE=GAAACTTCATTGATGCCAAAGAC
PRIMER_RIGHT_3_SEQUENCE=TTCTTTGAAGTGCTTTTTGATGG
PRIMER_LEFT_3=382,23
PRIMER_RIGHT_3=545,23
PRIMER_LEFT_3_TM=60.000
PRIMER_RIGHT_3_TM=60.611
PRIMER_LEFT_3_GC_PERCENT=39.130
PRIMER_RIGHT_3_GC_PERCENT=34.783
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_3_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=164
PRIMER_PAIR_PENALTY_4=0.6515
PRIMER_LEFT_4_PENALTY=0.533098
PRIMER_RIGHT_4_PENALTY=0.118384
PRIMER_LEFT_4_SEQUENCE=GTACTTCCTGGACTCTACCTCGG
PRIMER_RIGHT_4_SEQUENCE=GTCACAGTCATCACATACGCTGT
PRIMER_LEFT_4=360,23
PRIMER_RIGHT_4=658,23
PRIMER_LEFT_4_TM=60.533
PRIMER_RIGHT_4_TM=60.118
PRIMER_LEFT_4_GC_PERCENT=56.522
PRIMER_RIGHT_4_GC_PERCENT=47.826
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_4_MISPRIMING_SCORE=24.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_4_END_STABILITY=9.9000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=299
PRIMER_PAIR_PENALTY_5=0.6515
PRIMER_LEFT_5_PENALTY=0.533098
PRIMER_RIGHT_5_PENALTY=0.118384
PRIMER_LEFT_5_SEQUENCE=GGTACTTCCTGGACTCTACCTCG
PRIMER_RIGHT_5_SEQUENCE=GTCACAGTCATCACATACGCTGT
PRIMER_LEFT_5=359,23
PRIMER_RIGHT_5=658,23
PRIMER_LEFT_5_TM=60.533
PRIMER_RIGHT_5_TM=60.118
PRIMER_LEFT_5_GC_PERCENT=56.522
PRIMER_RIGHT_5_GC_PERCENT=47.826
PRIMER_LEFT_5_SELF_ANY=5.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=2.00
PRIMER_RIGHT_5_SELF_END=2.00
PRIMER_LEFT_5_MISPRIMING_SCORE=11.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_5_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_5_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_5_END_STABILITY=9.9000
PRIMER_RIGHT_5_END_STABILITY=7.9000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_5=300
PRIMER_PAIR_PENALTY_6=0.8158
PRIMER_LEFT_6_PENALTY=0.697424
PRIMER_RIGHT_6_PENALTY=0.118384
PRIMER_LEFT_6_SEQUENCE=AGGATATCACCTACCTTCGCATC
PRIMER_RIGHT_6_SEQUENCE=GTCACAGTCATCACATACGCTGT
PRIMER_LEFT_6=475,23
PRIMER_RIGHT_6=658,23
PRIMER_LEFT_6_TM=60.697
PRIMER_RIGHT_6_TM=60.118
PRIMER_LEFT_6_GC_PERCENT=47.826
PRIMER_RIGHT_6_GC_PERCENT=47.826
PRIMER_LEFT_6_SELF_ANY=6.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=2.00
PRIMER_LEFT_6_MISPRIMING_SCORE=11.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_6_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_6_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_6_END_STABILITY=8.1000
PRIMER_RIGHT_6_END_STABILITY=7.9000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_6=184
PRIMER_PAIR_PENALTY_7=0.8387
PRIMER_LEFT_7_PENALTY=0.000148
PRIMER_RIGHT_7_PENALTY=0.838538
PRIMER_LEFT_7_SEQUENCE=GAAACTTCATTGATGCCAAAGAC
PRIMER_RIGHT_7_SEQUENCE=TTTGAAGTGCTTTTTGATGGGTA
PRIMER_LEFT_7=382,23
PRIMER_RIGHT_7=542,23
PRIMER_LEFT_7_TM=60.000
PRIMER_RIGHT_7_TM=60.839
PRIMER_LEFT_7_GC_PERCENT=39.130
PRIMER_RIGHT_7_GC_PERCENT=34.783
PRIMER_LEFT_7_SELF_ANY=5.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=2.00
PRIMER_RIGHT_7_SELF_END=2.00
PRIMER_LEFT_7_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_7_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_7_MISPRIMING_SCORE=24.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_7_END_STABILITY=6.4000
PRIMER_RIGHT_7_END_STABILITY=8.4000
PRIMER_PAIR_7_COMPL_ANY=6.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_7=161
PRIMER_PAIR_PENALTY_8=0.8533
PRIMER_LEFT_8_PENALTY=0.734897
PRIMER_RIGHT_8_PENALTY=0.118384
PRIMER_LEFT_8_SEQUENCE=AGGTACCCATCAAAAAGCACTTC
PRIMER_RIGHT_8_SEQUENCE=GTCACAGTCATCACATACGCTGT
PRIMER_LEFT_8=517,23
PRIMER_RIGHT_8=658,23
PRIMER_LEFT_8_TM=60.735
PRIMER_RIGHT_8_TM=60.118
PRIMER_LEFT_8_GC_PERCENT=43.478
PRIMER_RIGHT_8_GC_PERCENT=47.826
PRIMER_LEFT_8_SELF_ANY=6.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=2.00
PRIMER_RIGHT_8_SELF_END=2.00
PRIMER_LEFT_8_MISPRIMING_SCORE=12.00, MER35 a consensus
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_8_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_8_END_STABILITY=6.4000
PRIMER_RIGHT_8_END_STABILITY=7.9000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_8=142
PRIMER_PAIR_PENALTY_9=0.8633
PRIMER_LEFT_9_PENALTY=0.744873
PRIMER_RIGHT_9_PENALTY=0.118384
PRIMER_LEFT_9_SEQUENCE=GCCGAAATAAGATCACACACATC
PRIMER_RIGHT_9_SEQUENCE=GTCACAGTCATCACATACGCTGT
PRIMER_LEFT_9=418,23
PRIMER_RIGHT_9=658,23
PRIMER_LEFT_9_TM=60.745
PRIMER_RIGHT_9_TM=60.118
PRIMER_LEFT_9_GC_PERCENT=43.478
PRIMER_RIGHT_9_GC_PERCENT=47.826
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=2.00
PRIMER_LEFT_9_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_9_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_9_END_STABILITY=6.3000
PRIMER_RIGHT_9_END_STABILITY=7.9000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_9=241
PRIMER_PAIR_PENALTY_10=0.9532
PRIMER_LEFT_10_PENALTY=0.342192
PRIMER_RIGHT_10_PENALTY=0.610986
PRIMER_LEFT_10_SEQUENCE=TCATCTCTATCCATGAGTCACCC
PRIMER_RIGHT_10_SEQUENCE=TTCTTTGAAGTGCTTTTTGATGG
PRIMER_LEFT_10=439,23
PRIMER_RIGHT_10=545,23
PRIMER_LEFT_10_TM=60.342
PRIMER_RIGHT_10_TM=60.611
PRIMER_LEFT_10_GC_PERCENT=47.826
PRIMER_RIGHT_10_GC_PERCENT=34.783
PRIMER_LEFT_10_SELF_ANY=5.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=3.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_10_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_10_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_10_END_STABILITY=9.4000
PRIMER_RIGHT_10_END_STABILITY=8.1000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_10=107
PRIMER_PAIR_PENALTY_11=0.9569
PRIMER_LEFT_11_PENALTY=0.838538
PRIMER_RIGHT_11_PENALTY=0.118384
PRIMER_LEFT_11_SEQUENCE=TACCCATCAAAAAGCACTTCAAA
PRIMER_RIGHT_11_SEQUENCE=GTCACAGTCATCACATACGCTGT
PRIMER_LEFT_11=520,23
PRIMER_RIGHT_11=658,23
PRIMER_LEFT_11_TM=60.839
PRIMER_RIGHT_11_TM=60.118
PRIMER_LEFT_11_GC_PERCENT=34.783
PRIMER_RIGHT_11_GC_PERCENT=47.826
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=2.00
PRIMER_RIGHT_11_SELF_END=2.00
PRIMER_LEFT_11_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_11_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_11_END_STABILITY=7.3000
PRIMER_RIGHT_11_END_STABILITY=7.9000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_11=139
PRIMER_PAIR_PENALTY_12=0.9701
PRIMER_LEFT_12_PENALTY=0.000148
PRIMER_RIGHT_12_PENALTY=0.969917
PRIMER_LEFT_12_SEQUENCE=GAAACTTCATTGATGCCAAAGAC
PRIMER_RIGHT_12_SEQUENCE=TCATCACATACGCTGTCACAATC
PRIMER_LEFT_12=382,23
PRIMER_RIGHT_12=651,23
PRIMER_LEFT_12_TM=60.000
PRIMER_RIGHT_12_TM=60.970
PRIMER_LEFT_12_GC_PERCENT=39.130
PRIMER_RIGHT_12_GC_PERCENT=43.478
PRIMER_LEFT_12_SELF_ANY=5.00
PRIMER_RIGHT_12_SELF_ANY=5.00
PRIMER_LEFT_12_SELF_END=2.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_12_MISPRIMING_SCORE=12.00, MER4C a consensus
PRIMER_PAIR_12_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_12_END_STABILITY=6.4000
PRIMER_RIGHT_12_END_STABILITY=6.9000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_12=270
PRIMER_PAIR_PENALTY_13=1.0950
PRIMER_LEFT_13_PENALTY=0.125045
PRIMER_RIGHT_13_PENALTY=0.969917
PRIMER_LEFT_13_SEQUENCE=CCGAAATAAGATCACACACATCA
PRIMER_RIGHT_13_SEQUENCE=TCATCACATACGCTGTCACAATC
PRIMER_LEFT_13=419,23
PRIMER_RIGHT_13=651,23
PRIMER_LEFT_13_TM=59.875
PRIMER_RIGHT_13_TM=60.970
PRIMER_LEFT_13_GC_PERCENT=39.130
PRIMER_RIGHT_13_GC_PERCENT=43.478
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=5.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_13_MISPRIMING_SCORE=12.00, MER4C a consensus
PRIMER_PAIR_13_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_13_END_STABILITY=6.9000
PRIMER_RIGHT_13_END_STABILITY=6.9000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_13=233
PRIMER_PAIR_PENALTY_14=1.1227
PRIMER_LEFT_14_PENALTY=1.004359
PRIMER_RIGHT_14_PENALTY=0.118384
PRIMER_LEFT_14_SEQUENCE=ACTTCATTGATGCCAAAGACCT
PRIMER_RIGHT_14_SEQUENCE=GTCACAGTCATCACATACGCTGT
PRIMER_LEFT_14=385,22
PRIMER_RIGHT_14=658,23
PRIMER_LEFT_14_TM=60.004
PRIMER_RIGHT_14_TM=60.118
PRIMER_LEFT_14_GC_PERCENT=40.909
PRIMER_RIGHT_14_GC_PERCENT=47.826
PRIMER_LEFT_14_SELF_ANY=5.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=2.00
PRIMER_RIGHT_14_SELF_END=2.00
PRIMER_LEFT_14_MISPRIMING_SCORE=12.00, LTR5 LTR from human endogenous retrovirus 5' LTR, clone HERV-K18
PRIMER_RIGHT_14_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_14_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_14_END_STABILITY=7.6000
PRIMER_RIGHT_14_END_STABILITY=7.9000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_14=274
PRIMER_PAIR_PENALTY_15=1.1441
PRIMER_LEFT_15_PENALTY=0.533098
PRIMER_RIGHT_15_PENALTY=0.610986
PRIMER_LEFT_15_SEQUENCE=GTACTTCCTGGACTCTACCTCGG
PRIMER_RIGHT_15_SEQUENCE=TTCTTTGAAGTGCTTTTTGATGG
PRIMER_LEFT_15=360,23
PRIMER_RIGHT_15=545,23
PRIMER_LEFT_15_TM=60.533
PRIMER_RIGHT_15_TM=60.611
PRIMER_LEFT_15_GC_PERCENT=56.522
PRIMER_RIGHT_15_GC_PERCENT=34.783
PRIMER_LEFT_15_SELF_ANY=5.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=2.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_15_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_15_MISPRIMING_SCORE=22.00, L1PA11 3'-end of L1 repeat (subfamily L1PA11) - a consensus sequence
PRIMER_LEFT_15_END_STABILITY=9.9000
PRIMER_RIGHT_15_END_STABILITY=8.1000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_15=186
PRIMER_PAIR_PENALTY_16=1.1441
PRIMER_LEFT_16_PENALTY=0.533098
PRIMER_RIGHT_16_PENALTY=0.610986
PRIMER_LEFT_16_SEQUENCE=GGTACTTCCTGGACTCTACCTCG
PRIMER_RIGHT_16_SEQUENCE=TTCTTTGAAGTGCTTTTTGATGG
PRIMER_LEFT_16=359,23
PRIMER_RIGHT_16=545,23
PRIMER_LEFT_16_TM=60.533
PRIMER_RIGHT_16_TM=60.611
PRIMER_LEFT_16_GC_PERCENT=56.522
PRIMER_RIGHT_16_GC_PERCENT=34.783
PRIMER_LEFT_16_SELF_ANY=5.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=2.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_MISPRIMING_SCORE=11.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_16_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_16_MISPRIMING_SCORE=21.00, L1PA11 3'-end of L1 repeat (subfamily L1PA11) - a consensus sequence
PRIMER_LEFT_16_END_STABILITY=9.9000
PRIMER_RIGHT_16_END_STABILITY=8.1000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_16=187
PRIMER_PAIR_PENALTY_17=1.1722
PRIMER_LEFT_17_PENALTY=0.000148
PRIMER_RIGHT_17_PENALTY=1.172020
PRIMER_LEFT_17_SEQUENCE=GAAACTTCATTGATGCCAAAGAC
PRIMER_RIGHT_17_SEQUENCE=TCACAGTCATCACATACGCTGTC
PRIMER_LEFT_17=382,23
PRIMER_RIGHT_17=657,23
PRIMER_LEFT_17_TM=60.000
PRIMER_RIGHT_17_TM=61.172
PRIMER_LEFT_17_GC_PERCENT=39.130
PRIMER_RIGHT_17_GC_PERCENT=47.826
PRIMER_LEFT_17_SELF_ANY=5.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=2.00
PRIMER_RIGHT_17_SELF_END=2.00
PRIMER_LEFT_17_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_17_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_17_MISPRIMING_SCORE=24.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_17_END_STABILITY=6.4000
PRIMER_RIGHT_17_END_STABILITY=6.4000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_17=276
PRIMER_PAIR_PENALTY_18=1.1769
PRIMER_LEFT_18_PENALTY=1.058487
PRIMER_RIGHT_18_PENALTY=0.118384
PRIMER_LEFT_18_SEQUENCE=GTATCAACTTCATCCACTGCTGC
PRIMER_RIGHT_18_SEQUENCE=GTCACAGTCATCACATACGCTGT
PRIMER_LEFT_18=547,23
PRIMER_RIGHT_18=658,23
PRIMER_LEFT_18_TM=61.058
PRIMER_RIGHT_18_TM=60.118
PRIMER_LEFT_18_GC_PERCENT=47.826
PRIMER_RIGHT_18_GC_PERCENT=47.826
PRIMER_LEFT_18_SELF_ANY=5.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=3.00
PRIMER_RIGHT_18_SELF_END=2.00
PRIMER_LEFT_18_MISPRIMING_SCORE=12.00, L1MB7 3'-end of L1 repeat (subfamily L1MB7) - a consensus sequence
PRIMER_RIGHT_18_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_18_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_18_END_STABILITY=9.7000
PRIMER_RIGHT_18_END_STABILITY=7.9000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_18=112
PRIMER_PAIR_PENALTY_19=1.1807
PRIMER_LEFT_19_PENALTY=0.342192
PRIMER_RIGHT_19_PENALTY=0.838538
PRIMER_LEFT_19_SEQUENCE=TCATCTCTATCCATGAGTCACCC
PRIMER_RIGHT_19_SEQUENCE=TTTGAAGTGCTTTTTGATGGGTA
PRIMER_LEFT_19=439,23
PRIMER_RIGHT_19=542,23
PRIMER_LEFT_19_TM=60.342
PRIMER_RIGHT_19_TM=60.839
PRIMER_LEFT_19_GC_PERCENT=47.826
PRIMER_RIGHT_19_GC_PERCENT=34.783
PRIMER_LEFT_19_SELF_ANY=5.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=3.00
PRIMER_RIGHT_19_SELF_END=2.00
PRIMER_LEFT_19_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_19_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_19_MISPRIMING_SCORE=24.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_19_END_STABILITY=9.4000
PRIMER_RIGHT_19_END_STABILITY=8.4000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_19=104
=