PRIMER_SEQUENCE_ID=NM_080876
SEQUENCE=AGCGGGGTGGGATAAACGGAGCTGGACGACTCAGTCTCTTGGTCTGTGGCTGCTGCGGTTACCTGGATGGGCGAGCACCTCTGAGGCTGGCTTTGTTACCTGGGCAATAAGGGACTAGCAGTTCAGCCGTTTTCTATGCCTGCTGGATTTGTTTGTATTTGTTCCCAGCCACTGCTCATGTAATGTACTCCCTTAACCAGGAAATTAAAGCATTCTCCCGGAATAATCTCAGGAAGCAATGCACCAGGGTGACAACGCTAACTGGAAAGAAAATTATAGAAACATGGAAAGATGCCAGAATTCATGTTGTGGAAGAAGTAGAGCCGAGCAGTGGGGGTGGTTGTGGTTATGTGCAGGACCTTAGCTCGGACCTGCAAGTTGGCGTTATTAAGCCATGGTTGCTCCTAGGGTCACAAGATGCTGCTCATGATTTGGATACACTGAAAAAGAATAAGGTGACTCATATTCTTAATGTTGCATATGGAGTTGAAAATGCTTTCCTCAGTGACTTTACATATAAGAGCATTTCTATATTGGATCTGCCTGAAACCAACATCCTGTCTTATTTTCCAGAATGTTTTGAATTTATTGAAGAAGCAAAAAGAAAAGATGGAGTGGTTCTTGTTCATTGTAATGCAGGCGTTTCCAGGGCTGCTGCAATTGTAATAGGTTTCCTGATGAATTCTGAACAAACCTCATTTACCAGTGCTTTTTCTTTGGTGAAAAATGCAAGACCTTCCATATGTCCAAATTCTGGCTTCATGGAGCAGCTTCGTACATATCAAGAGGGCAAAGAAAGCAATAAGTGTGACAGAATACAGGAGAACAGTTCATGAGTTGCATTGTAGCAGACAATGGACAACTGTAGTTTCTGAATTGACTTCTATAGCCATCTTTTCCCTTTTTTGGAGAGTAGACTAGCAAAACTCCCTTTTTTCTCTTGCCTTTTTTATGCATAAATGGAGGTCAATCTGATTGTCCTGACCTACTGTATAAGTAAATTTCAAATGTCATTACTTTCTCTTTGTTATTATAATGTGTGATTAAATGCTTTTTTAAATTGCTAAGGGAAAATAACAAAGTGTTAGTAATCATTGCTTTCTGTAGAAGAAAAAGGTTTAAATTTAAAATGTGTATTTAGAGCATGGAGAGAATCATTTAATGAAGCCTATTTTTCTGTTCCAGCAACTGTTTTTATGAAAGAAAAACTTGAAATGCTACTTAACTTACTATTTCAAAGGGAAGTATATTTCTTTTTAAAGAATCTTTTTAAGATTAAAAATGAATACCTTAAATCTCCCAAAAGAGATAACCAGTATCTGAAGGTTTATACAATATTTACACAGTGGCTACAATATTCACAAAATTCTTATGTTCTCTTATGAAAAATATACACTTTTCATTTTGTCTTCTTCTTTTTTTTTTTTTTTTAGATGGAGTTTGGCTCTTGTCACCCAGGCTGGAGTGCAGTGGCGCAATCTCGGCTCACTGCAGCCTCTGCCTCCCGGGTTCAAGCGATTCTCCTGCTCCAGCCTCCCATATAGCTGGGATTATAGGTGCCTGCCACCATGCCCGGCTAATTTTTGTGTTTTTTAGTAGAGATGGAGTCTCACCACATAGGCCAGGCAGTCTCGAACTCCTGACCTCAGGTGATCCACCCACCTTGGCCTCTCTAAATGCTGGGATTGCAGGCGTGAGCCACTGCATCCGACCTCATTTTGTTTTTTTCAATTCTGTTATCGGCATTTCTTTCTTTTTTTTTTTAATGTATAGGTGTTCTGTCATACGTTATTGTGAGCTTTGGTTCTTTGAACAGGTGATCTATTCTCTTACATTTTGTGCTTTTTATCTTGGGTGCCTAGCATTCTGTTTTTTTGATCCTATGTCATAATTTTTTGAGAAAATGTTTGATACTGCAAGATCATTGTTTAGTGGTCTGTTTTCTACTTCAGTATTCTACCAGAGAACATATTTTTTAGGGGAGAAGGGTTATGGTTTTGTAAGAAACTTTAAATATACTTTTAGGAGACCCACAATCATTTACAGTTTTATCTTTGGCCTTTGAATAGGTTCTTTTTTCTTTAATGTCTCCGTATTTGCAGAACTGAGCTCTGTACGTTATAAATTTGCTTAAAGATTTTGATTTTTTCTTCAGAATATGGTATACTTAAAATTAATTAATTAAGACAGTTTCTTTTTTCTTTTCTTTTTTTTTTTTTCGAGACAGGGTCTTGCTCTGTCACCTAGGCTAGAGTGGAGTGGCATGAACACAACGCACTGCAGCCTCTACCTCCTGGGCTCAAGCAGTCCTCCCACCTCAGTCTCCTGAGTAGCTGAGACTACAGCTGTGCGCCACCACATCTGGCTAATTTTATTTTTATTTTTGTAGAGACAGGAGTCTTGCCATGTTGCCCAGGCTGATCTCAAACTCCTAGGCTTAAGTGATCCTCCCGCCTTAGCCTCCCAAGTGCTAGGATTACAGGCATCAGCCACCACGCCTGGCCTGATAGTTTCCACATAGTTTTTCATGACTTAGAAGATATCATTGCCATGTAGGTTTTATTTTTAAAAGTCTTGAGCTCTTCTTAAATATACATTATAAAGAGCAAGCATAGATGGAAAGTGCATATTGAACACATCTGTGCATTTGAGAAGGCAGGCTTTCATATGCTGTCATGAGCAAAAAGTAGAAGTGATAAATGCAAGTTATGTTTTGAGATTGCTTACACCGTGACAAGATGCCATCACTTGTGATGTTATGAACTGTTATAAACTCTTTGCTTCTGCTAAGAAGCAGTCTAAATATAATCAACGTACAGTGAGCTCTTTGAAGCCAACCAAGGCAGGCAGAACCCTCTTAAATATGAGACTGATGTATAGGTTCTCTCTATGGTCAGTATCACTGGATAAGCCTTCTCCTTTCATAAGAAAGGATGTATTTAAATACATTTTATTTTGCTAAATCCTGTTTAGTTTTCAGCCATTAAATGTCACATGAAGCCTTGATAGAATTTGTTCATAATGGCTCATGTATTATTATTATTTTTGAGATGGAGTTTTGCTCTTAATGATATTATTATTATTTGAGACAGAGTTTTGCTCTTATTGCCCAGGCTGGAGTGCAGTGGCGTGATCTTGGCTCACTGCGGCCTCTGCCTCCCGGCTTCAAGCGATTCTCCTGTATCGGCCTCCCAGGTAGCTGGGATTATAGGCGTGTGCCACCATGCCCAGCAAATTTTGTATTTTTTTTTGGTAGAGACAGGGTTCAACCGTGTTGGTCAGGCTGGTCTCGAACTCCTGACCGCAAGTGATCCACACGCCTTGGTCTCCCAAAGTGCTGGGATTACGGTGTTAGCCACTACACCTGGCCTGGATTGTGTATTATTTATGTCTGTAGTTTATATGCTTAGTTGTTGCCTATAGTGATCTGGGTAAGATTCAAACATTTATCTTTTGTATCTTCTACAGAAGTGCTTTGAGCATCAAATTTGTTTTAACGTTAAATTGTAGTTTGCTGTATTAAAATAGATCAATGAATATAATTCAGTCTTTTAGGGGCACCAAGTAAAGCATAAGGTCATATATACTATACAATATGTTTATTGCACTTCCCATGGGGATAAATCACTCTGTCATTCCTAGTTATTTTAAAAAAAGAACTTTATGATTACGGTCCTCTTTCTCACATACTGCAAACTTAAAAGATACATACACCAAATATAGGTCTGTTTTAAAGGAGAGGAAAAATAGTTCAAGGAGTTTTTGCCTCTTTGTTTTTAAATAGATTATTCTGCCATTTTTTAAAGGAAAGGGAAAATGAAAACAGCATGTCTTTTTAAACATTGAAAAGAAATATGGAGGCTTTAAACCGCAAACTGAAAAAGCTGAGTAGAACAAAGGCAGTGGAGCATACAATACAATTCTATTTATTTGAGGTTAATTGAAGTTAATAATTTTTAAATTTTTTTCTAATTTTATGCCTTAAAATAGGTTTCTGATTACATAAAATTTGAATAGAACACTCAAGAAGTATGTAGATTTTTGAAAAACCCAAACAATATTCTTCTTAGATTTTTGGAGGCAAAATTTAGACCAGTATACTAATTCAAAAGACAAATATTTCAGAGGAAGTGGAGTATAGTATTTAACATTTTGTTTGCAATTTTACTTTTTCTTCCCTTCTTCCCTATTGAACCTTGATTAAAATGTATGAATACAATACTTGGATTTTTTTTTCAGCTTAGACTCTGATAATTTCAGTGGTAGTGGGGAAACCTGAATTTGTCAGCATGATTATTTGTAACTCAGATTGTTCAAATGTATATAGAAGGTCCCCTTCAAGTGTTGTTTCTCCAAGTAGTCATAAATGTAGCAAGTATAAATCAGAATATCATCTTATAATCTTACAGAGTTAACCTAATGAGTCAATACCTTTATAAGCAAAACCATGATAAATTTAAAAACAACAGAAATGTGGCATGATTACTTATTTATTTGTTCCAGGTGACTCTAAATAGTTGATTTTTCTGCCTGAAGGATCCTGAACATATTTAGTTACCCTGTAGTTATTTAGGAATTTAAACCAGAAGACTTTGAAGTTGTATATTCTTGCAATGCATAAAATGACATTATAATCAACAAACTTATGGTTTGTTTCAATTGATGCACACAAAAAATAACTTATAGTTAGACTTTTCTATTTCAAGTAAAGCTTTGAGTTACTTATTTTTATACATATTCATCATGTACCCTTTATTGTTTCTAGGTGTTGATATACTTTAATAAAAATGATATGTTTAATATTTTCCCATTAATATATTTCTTTGAAGCTATTTGTTTTAAAAGATGTAACACTTATTTGCTGTCAAATTCTGAATTAAAAAATCTGTTGGTGGCCGGGCGCAGTGGCTCATGCCTGTGGTCCCAACATTTTGGGAGGCCAAGGAGATGGATCACTTGAGGCCAGGAGTTCGAGACCAGCCTGGCCAACATGGCAAAACCCCATCACTAAAAATACAAAAATTACCCGGGCATGGTGGTGGGTGCCTGTAATCCCAGCTTCTTAGGAGGCTGAGGTAGGAGAATTGCTTGACCCTGGGAGGTGGAGGTGGCAGTGAGCCGAGATAGCACCACTGCACTTCTAGTTGACAGAGTGAGACTCTGACTCAAAAAAAAAAAAAAAA
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 58111, GC content failed 6214, low tm 32548, high tm 9454, high any compl 31, high end compl 59, high repeat similarity 69, long poly-x seq 412, ok 9324
PRIMER_RIGHT_EXPLAIN=considered 58052, GC content failed 6270, low tm 32696, high tm 9206, high any compl 3, high end compl 6, high repeat similarity 13, long poly-x seq 429, ok 9429
PRIMER_PAIR_EXPLAIN=considered 1147, unacceptable product size 1074, high end compl 8, ok 65
PRIMER_PAIR_PENALTY=0.0226
PRIMER_LEFT_PENALTY=0.008837
PRIMER_RIGHT_PENALTY=0.013803
PRIMER_LEFT_SEQUENCE=GAGATGGAGTCTCACCACATAGG
PRIMER_RIGHT_SEQUENCE=AAGAACCTATTCAAAGGCCAAAG
PRIMER_LEFT=1598,23
PRIMER_RIGHT=2074,23
PRIMER_LEFT_TM=60.009
PRIMER_RIGHT_TM=60.014
PRIMER_LEFT_GC_PERCENT=52.174
PRIMER_RIGHT_GC_PERCENT=39.130
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_MISPRIMING_SCORE=24.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_END_STABILITY=7.1000
PRIMER_RIGHT_END_STABILITY=7.3000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=477
PRIMER_PAIR_PENALTY_1=0.0258
PRIMER_LEFT_1_PENALTY=0.008837
PRIMER_RIGHT_1_PENALTY=0.016975
PRIMER_LEFT_1_SEQUENCE=GAGATGGAGTCTCACCACATAGG
PRIMER_RIGHT_1_SEQUENCE=AGAAATGCCGATAACAGAATTGA
PRIMER_LEFT_1=1598,23
PRIMER_RIGHT_1=1749,23
PRIMER_LEFT_1_TM=60.009
PRIMER_RIGHT_1_TM=59.983
PRIMER_LEFT_1_GC_PERCENT=52.174
PRIMER_RIGHT_1_GC_PERCENT=34.783
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_1_MISPRIMING_SCORE=24.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_1_END_STABILITY=7.1000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=152
PRIMER_PAIR_PENALTY_2=0.0263
PRIMER_LEFT_2_PENALTY=0.000316
PRIMER_RIGHT_2_PENALTY=0.026007
PRIMER_LEFT_2_SEQUENCE=TTATTAAGCCATGGTTGCTCCTA
PRIMER_RIGHT_2_SEQUENCE=TGAACAAGAACCACTCCATCTTT
PRIMER_LEFT_2=384,23
PRIMER_RIGHT_2=627,23
PRIMER_LEFT_2_TM=60.000
PRIMER_RIGHT_2_TM=60.026
PRIMER_LEFT_2_GC_PERCENT=39.130
PRIMER_RIGHT_2_GC_PERCENT=39.130
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_2_MISPRIMING_SCORE=11.00, MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_PAIR_2_MISPRIMING_SCORE=21.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=244
PRIMER_PAIR_PENALTY_3=0.0561
PRIMER_LEFT_3_PENALTY=0.030113
PRIMER_RIGHT_3_PENALTY=0.026007
PRIMER_LEFT_3_SEQUENCE=GACGACTCAGTCTCTTGGTCTGT
PRIMER_RIGHT_3_SEQUENCE=TGAACAAGAACCACTCCATCTTT
PRIMER_LEFT_3=24,23
PRIMER_RIGHT_3=627,23
PRIMER_LEFT_3_TM=59.970
PRIMER_RIGHT_3_TM=60.026
PRIMER_LEFT_3_GC_PERCENT=52.174
PRIMER_RIGHT_3_GC_PERCENT=39.130
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_3_MISPRIMING_SCORE=11.00, MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_PAIR_3_MISPRIMING_SCORE=22.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=604
PRIMER_PAIR_PENALTY_4=0.0739
PRIMER_LEFT_4_PENALTY=0.000316
PRIMER_RIGHT_4_PENALTY=0.073619
PRIMER_LEFT_4_SEQUENCE=TTATTAAGCCATGGTTGCTCCTA
PRIMER_RIGHT_4_SEQUENCE=GCACTGGTAAATGAGGTTTGTTC
PRIMER_LEFT_4=384,23
PRIMER_RIGHT_4=708,23
PRIMER_LEFT_4_TM=60.000
PRIMER_RIGHT_4_TM=59.926
PRIMER_LEFT_4_GC_PERCENT=39.130
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, reverse MER3 Nonautonomous DNA transposon
PRIMER_PAIR_4_MISPRIMING_SCORE=21.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=325
PRIMER_PAIR_PENALTY_5=0.0780
PRIMER_LEFT_5_PENALTY=0.073619
PRIMER_RIGHT_5_PENALTY=0.004430
PRIMER_LEFT_5_SEQUENCE=AGAGCAAGCATAGATGGAAAGTG
PRIMER_RIGHT_5_SEQUENCE=AGGTGTAGTGGCTAACACCGTAA
PRIMER_LEFT_5=2602,23
PRIMER_RIGHT_5=3365,23
PRIMER_LEFT_5_TM=59.926
PRIMER_RIGHT_5_TM=59.996
PRIMER_LEFT_5_GC_PERCENT=43.478
PRIMER_RIGHT_5_GC_PERCENT=47.826
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=7.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_MISPRIMING_SCORE=11.00, L1MA2 3'-end of L1 repeat (subfamily L1MA2) - a consensus sequence
PRIMER_RIGHT_5_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_5_MISPRIMING_SCORE=21.00, MER4C a consensus
PRIMER_LEFT_5_END_STABILITY=6.7000
PRIMER_RIGHT_5_END_STABILITY=7.7000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_5=764
PRIMER_PAIR_PENALTY_6=0.0804
PRIMER_LEFT_6_PENALTY=0.073619
PRIMER_RIGHT_6_PENALTY=0.006805
PRIMER_LEFT_6_SEQUENCE=AGAGCAAGCATAGATGGAAAGTG
PRIMER_RIGHT_6_SEQUENCE=ATATTTAAGAGGGTTCTGCCTGC
PRIMER_LEFT_6=2602,23
PRIMER_RIGHT_6=2864,23
PRIMER_LEFT_6_TM=59.926
PRIMER_RIGHT_6_TM=60.007
PRIMER_LEFT_6_GC_PERCENT=43.478
PRIMER_RIGHT_6_GC_PERCENT=43.478
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=2.00
PRIMER_LEFT_6_MISPRIMING_SCORE=11.00, L1MA2 3'-end of L1 repeat (subfamily L1MA2) - a consensus sequence
PRIMER_RIGHT_6_MISPRIMING_SCORE=12.00, reverse MLT2D Interspersed repeat MLT2D - a consensus
PRIMER_PAIR_6_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_6_END_STABILITY=6.7000
PRIMER_RIGHT_6_END_STABILITY=9.7000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_6=263
PRIMER_PAIR_PENALTY_7=0.0996
PRIMER_LEFT_7_PENALTY=0.026007
PRIMER_RIGHT_7_PENALTY=0.073619
PRIMER_LEFT_7_SEQUENCE=AAAGATGGAGTGGTTCTTGTTCA
PRIMER_RIGHT_7_SEQUENCE=GCACTGGTAAATGAGGTTTGTTC
PRIMER_LEFT_7=605,23
PRIMER_RIGHT_7=708,23
PRIMER_LEFT_7_TM=60.026
PRIMER_RIGHT_7_TM=59.926
PRIMER_LEFT_7_GC_PERCENT=39.130
PRIMER_RIGHT_7_GC_PERCENT=43.478
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=2.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_MISPRIMING_SCORE=12.00, MER41 Medium reiteration sequence MER41
PRIMER_RIGHT_7_MISPRIMING_SCORE=12.00, reverse MER3 Nonautonomous DNA transposon
PRIMER_PAIR_7_MISPRIMING_SCORE=21.00, reverse MER39 a consensus
PRIMER_LEFT_7_END_STABILITY=6.7000
PRIMER_RIGHT_7_END_STABILITY=6.7000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_7=104
PRIMER_PAIR_PENALTY_8=0.1037
PRIMER_LEFT_8_PENALTY=0.030113
PRIMER_RIGHT_8_PENALTY=0.073619
PRIMER_LEFT_8_SEQUENCE=GACGACTCAGTCTCTTGGTCTGT
PRIMER_RIGHT_8_SEQUENCE=GCACTGGTAAATGAGGTTTGTTC
PRIMER_LEFT_8=24,23
PRIMER_RIGHT_8=708,23
PRIMER_LEFT_8_TM=59.970
PRIMER_RIGHT_8_TM=59.926
PRIMER_LEFT_8_GC_PERCENT=52.174
PRIMER_RIGHT_8_GC_PERCENT=43.478
PRIMER_LEFT_8_SELF_ANY=7.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=3.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, reverse MER3 Nonautonomous DNA transposon
PRIMER_PAIR_8_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_8_END_STABILITY=6.4000
PRIMER_RIGHT_8_END_STABILITY=6.7000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_8=685
PRIMER_PAIR_PENALTY_9=0.1047
PRIMER_LEFT_9_PENALTY=0.094334
PRIMER_RIGHT_9_PENALTY=0.010384
PRIMER_LEFT_9_SEQUENCE=TTGTTGCCTATAGTGATCTGGGT
PRIMER_RIGHT_9_SEQUENCE=TACTGGTCTAAATTTTGCCTCCA
PRIMER_LEFT_9=3410,23
PRIMER_RIGHT_9=4097,23
PRIMER_LEFT_9_TM=59.906
PRIMER_RIGHT_9_TM=60.010
PRIMER_LEFT_9_GC_PERCENT=43.478
PRIMER_RIGHT_9_GC_PERCENT=39.130
PRIMER_LEFT_9_SELF_ANY=6.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=2.00
PRIMER_LEFT_9_MISPRIMING_SCORE=11.00, L1MC2 3'-end of L1 repeat (subfamily L1MC2) - a consensus sequence
PRIMER_RIGHT_9_MISPRIMING_SCORE=11.00, reverse L1ME2 3'-end of L1 repeat (subfamily L1ME2) - a consensus sequence
PRIMER_PAIR_9_MISPRIMING_SCORE=22.00, reverse L1ME2 3'-end of L1 repeat (subfamily L1ME2) - a consensus sequence
PRIMER_LEFT_9_END_STABILITY=9.4000
PRIMER_RIGHT_9_END_STABILITY=8.2000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_9=688
PRIMER_PAIR_PENALTY_10=0.1047
PRIMER_LEFT_10_PENALTY=0.094334
PRIMER_RIGHT_10_PENALTY=0.010384
PRIMER_LEFT_10_SEQUENCE=GTTGTTGCCTATAGTGATCTGGG
PRIMER_RIGHT_10_SEQUENCE=TACTGGTCTAAATTTTGCCTCCA
PRIMER_LEFT_10=3409,23
PRIMER_RIGHT_10=4097,23
PRIMER_LEFT_10_TM=59.906
PRIMER_RIGHT_10_TM=60.010
PRIMER_LEFT_10_GC_PERCENT=47.826
PRIMER_RIGHT_10_GC_PERCENT=39.130
PRIMER_LEFT_10_SELF_ANY=6.00
PRIMER_RIGHT_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=2.00
PRIMER_LEFT_10_MISPRIMING_SCORE=12.00, L1MC2 3'-end of L1 repeat (subfamily L1MC2) - a consensus sequence
PRIMER_RIGHT_10_MISPRIMING_SCORE=11.00, reverse L1ME2 3'-end of L1 repeat (subfamily L1ME2) - a consensus sequence
PRIMER_PAIR_10_MISPRIMING_SCORE=22.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_10_END_STABILITY=9.7000
PRIMER_RIGHT_10_END_STABILITY=8.2000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_10=689
PRIMER_PAIR_PENALTY_11=0.1182
PRIMER_LEFT_11_PENALTY=0.000316
PRIMER_RIGHT_11_PENALTY=0.117879
PRIMER_LEFT_11_SEQUENCE=TTATTAAGCCATGGTTGCTCCTA
PRIMER_RIGHT_11_SEQUENCE=CATTTTCAACTCCATATGCAACA
PRIMER_LEFT_11=384,23
PRIMER_RIGHT_11=494,23
PRIMER_LEFT_11_TM=60.000
PRIMER_RIGHT_11_TM=59.882
PRIMER_LEFT_11_GC_PERCENT=39.130
PRIMER_RIGHT_11_GC_PERCENT=34.783
PRIMER_LEFT_11_SELF_ANY=6.00
PRIMER_RIGHT_11_SELF_ANY=6.00
PRIMER_LEFT_11_SELF_END=2.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, MER4C a consensus
PRIMER_PAIR_11_MISPRIMING_SCORE=23.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_11_END_STABILITY=7.2000
PRIMER_RIGHT_11_END_STABILITY=7.0000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_11=111
PRIMER_PAIR_PENALTY_12=0.1195
PRIMER_LEFT_12_PENALTY=0.010384
PRIMER_RIGHT_12_PENALTY=0.109069
PRIMER_LEFT_12_SEQUENCE=TGGAGGCAAAATTTAGACCAGTA
PRIMER_RIGHT_12_SEQUENCE=ATCATGCTGACAAATTCAGGTTT
PRIMER_LEFT_12=4075,23
PRIMER_RIGHT_12=4293,23
PRIMER_LEFT_12_TM=60.010
PRIMER_RIGHT_12_TM=59.891
PRIMER_LEFT_12_GC_PERCENT=39.130
PRIMER_RIGHT_12_GC_PERCENT=34.783
PRIMER_LEFT_12_SELF_ANY=6.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=2.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_MISPRIMING_SCORE=11.00, reverse L1ME2 3'-end of L1 repeat (subfamily L1ME2) - a consensus sequence
PRIMER_RIGHT_12_MISPRIMING_SCORE=11.00, LTR11 LTR from human HTLV-I related endogenous retroviral sequence (HRES-1/1)
PRIMER_PAIR_12_MISPRIMING_SCORE=22.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_12_END_STABILITY=5.7000
PRIMER_RIGHT_12_END_STABILITY=8.2000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_12=219
PRIMER_PAIR_PENALTY_13=0.1195
PRIMER_LEFT_13_PENALTY=0.010384
PRIMER_RIGHT_13_PENALTY=0.109069
PRIMER_LEFT_13_SEQUENCE=TGGAGGCAAAATTTAGACCAGTA
PRIMER_RIGHT_13_SEQUENCE=AATCATGCTGACAAATTCAGGTT
PRIMER_LEFT_13=4075,23
PRIMER_RIGHT_13=4294,23
PRIMER_LEFT_13_TM=60.010
PRIMER_RIGHT_13_TM=59.891
PRIMER_LEFT_13_GC_PERCENT=39.130
PRIMER_RIGHT_13_GC_PERCENT=34.783
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=2.00
PRIMER_RIGHT_13_SELF_END=2.00
PRIMER_LEFT_13_MISPRIMING_SCORE=11.00, reverse L1ME2 3'-end of L1 repeat (subfamily L1ME2) - a consensus sequence
PRIMER_RIGHT_13_MISPRIMING_SCORE=12.00, MER41 Medium reiteration sequence MER41
PRIMER_PAIR_13_MISPRIMING_SCORE=21.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_13_END_STABILITY=5.7000
PRIMER_RIGHT_13_END_STABILITY=7.9000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_13=220
PRIMER_PAIR_PENALTY_14=0.1392
PRIMER_LEFT_14_PENALTY=0.030113
PRIMER_RIGHT_14_PENALTY=0.109069
PRIMER_LEFT_14_SEQUENCE=GACGACTCAGTCTCTTGGTCTGT
PRIMER_RIGHT_14_SEQUENCE=ACAACATGAATTCTGGCATCTTT
PRIMER_LEFT_14=24,23
PRIMER_RIGHT_14=309,23
PRIMER_LEFT_14_TM=59.970
PRIMER_RIGHT_14_TM=59.891
PRIMER_LEFT_14_GC_PERCENT=52.174
PRIMER_RIGHT_14_GC_PERCENT=34.783
PRIMER_LEFT_14_SELF_ANY=7.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=3.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_14_MISPRIMING_SCORE=11.00, reverse MER11A a consensus
PRIMER_PAIR_14_MISPRIMING_SCORE=21.00, Tigger2 Autonomous DNA transposon
PRIMER_LEFT_14_END_STABILITY=6.4000
PRIMER_RIGHT_14_END_STABILITY=7.0000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_14=286
PRIMER_PAIR_PENALTY_15=0.1401
PRIMER_LEFT_15_PENALTY=0.133335
PRIMER_RIGHT_15_PENALTY=0.006805
PRIMER_LEFT_15_SEQUENCE=ATCTTTGGCCTTTGAATAGGTTC
PRIMER_RIGHT_15_SEQUENCE=ATATTTAAGAGGGTTCTGCCTGC
PRIMER_LEFT_15=2050,23
PRIMER_RIGHT_15=2864,23
PRIMER_LEFT_15_TM=59.867
PRIMER_RIGHT_15_TM=60.007
PRIMER_LEFT_15_GC_PERCENT=39.130
PRIMER_RIGHT_15_GC_PERCENT=43.478
PRIMER_LEFT_15_SELF_ANY=5.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=2.00
PRIMER_RIGHT_15_SELF_END=2.00
PRIMER_LEFT_15_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_15_MISPRIMING_SCORE=12.00, reverse MLT2D Interspersed repeat MLT2D - a consensus
PRIMER_PAIR_15_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_15_END_STABILITY=7.9000
PRIMER_RIGHT_15_END_STABILITY=9.7000
PRIMER_PAIR_15_COMPL_ANY=6.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_15=815
PRIMER_PAIR_PENALTY_16=0.1411
PRIMER_LEFT_16_PENALTY=0.073619
PRIMER_RIGHT_16_PENALTY=0.067516
PRIMER_LEFT_16_SEQUENCE=AGAGCAAGCATAGATGGAAAGTG
PRIMER_RIGHT_16_SEQUENCE=TTGATGCTCAAAGCACTTCTGTA
PRIMER_LEFT_16=2602,23
PRIMER_RIGHT_16=3485,23
PRIMER_LEFT_16_TM=59.926
PRIMER_RIGHT_16_TM=60.068
PRIMER_LEFT_16_GC_PERCENT=43.478
PRIMER_RIGHT_16_GC_PERCENT=39.130
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=6.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=2.00
PRIMER_LEFT_16_MISPRIMING_SCORE=11.00, L1MA2 3'-end of L1 repeat (subfamily L1MA2) - a consensus sequence
PRIMER_RIGHT_16_MISPRIMING_SCORE=11.00, MARINER1 Human mariner
PRIMER_PAIR_16_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_16_END_STABILITY=6.7000
PRIMER_RIGHT_16_END_STABILITY=5.7000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_16=884
PRIMER_PAIR_PENALTY_17=0.1422
PRIMER_LEFT_17_PENALTY=0.008837
PRIMER_RIGHT_17_PENALTY=0.133335
PRIMER_LEFT_17_SEQUENCE=GAGATGGAGTCTCACCACATAGG
PRIMER_RIGHT_17_SEQUENCE=GAACCTATTCAAAGGCCAAAGAT
PRIMER_LEFT_17=1598,23
PRIMER_RIGHT_17=2072,23
PRIMER_LEFT_17_TM=60.009
PRIMER_RIGHT_17_TM=59.867
PRIMER_LEFT_17_GC_PERCENT=52.174
PRIMER_RIGHT_17_GC_PERCENT=39.130
PRIMER_LEFT_17_SELF_ANY=5.00
PRIMER_RIGHT_17_SELF_ANY=5.00
PRIMER_LEFT_17_SELF_END=2.00
PRIMER_RIGHT_17_SELF_END=2.00
PRIMER_LEFT_17_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_17_MISPRIMING_SCORE=12.00, MER21B a consensus
PRIMER_PAIR_17_MISPRIMING_SCORE=23.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_17_END_STABILITY=7.1000
PRIMER_RIGHT_17_END_STABILITY=6.6000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_17=475
PRIMER_PAIR_PENALTY_18=0.1450
PRIMER_LEFT_18_PENALTY=0.008837
PRIMER_RIGHT_18_PENALTY=0.136114
PRIMER_LEFT_18_SEQUENCE=GAGATGGAGTCTCACCACATAGG
PRIMER_RIGHT_18_SEQUENCE=AATGCCGATAACAGAATTGAAAA
PRIMER_LEFT_18=1598,23
PRIMER_RIGHT_18=1746,23
PRIMER_LEFT_18_TM=60.009
PRIMER_RIGHT_18_TM=59.864
PRIMER_LEFT_18_GC_PERCENT=52.174
PRIMER_RIGHT_18_GC_PERCENT=30.435
PRIMER_LEFT_18_SELF_ANY=5.00
PRIMER_RIGHT_18_SELF_ANY=6.00
PRIMER_LEFT_18_SELF_END=2.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_18_MISPRIMING_SCORE=12.00, MER7B Nonautonomous DNA transposon
PRIMER_PAIR_18_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_18_END_STABILITY=7.1000
PRIMER_RIGHT_18_END_STABILITY=7.3000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_18=149
PRIMER_PAIR_PENALTY_19=0.1450
PRIMER_LEFT_19_PENALTY=0.008837
PRIMER_RIGHT_19_PENALTY=0.136114
PRIMER_LEFT_19_SEQUENCE=GAGATGGAGTCTCACCACATAGG
PRIMER_RIGHT_19_SEQUENCE=AAATGCCGATAACAGAATTGAAA
PRIMER_LEFT_19=1598,23
PRIMER_RIGHT_19=1747,23
PRIMER_LEFT_19_TM=60.009
PRIMER_RIGHT_19_TM=59.864
PRIMER_LEFT_19_GC_PERCENT=52.174
PRIMER_RIGHT_19_GC_PERCENT=30.435
PRIMER_LEFT_19_SELF_ANY=5.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=2.00
PRIMER_RIGHT_19_SELF_END=2.00
PRIMER_LEFT_19_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_19_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_19_MISPRIMING_SCORE=24.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_19_END_STABILITY=7.1000
PRIMER_RIGHT_19_END_STABILITY=7.3000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_19=150
=