PRIMER_SEQUENCE_ID=NM_130435
SEQUENCE=GTGCAGCAGAGGGCAGCTGAGAGGCTGGGTGGCTGGGCCTGGGAGACACACAGAGGCCAGGCCTTAGCGCGGCTCAGCCATGAGCAACAGGAGTAGCTTTTCCCGGCTCACCTGGTTCAGGAAGCAGAGGAAAGCTGTGGTCAGCACCAGCGACAAGAAGATGCCCAACGGAATCTTGGAGGAGCAAGAGCAGCAAAGGGTGATGCTGCTCAGCAGGTCACCCTCAGGGCCCAAGAAGTATTTTCCCATCCCCGTGGAGCACCTGGAGGAGGAGATCCGTATCAGATCCGCCGACGACTGCAAGCAGTTTCGGGAGGAGTTCAACTCATTGCCATCTGGACACATACAAGGAACTTTTGAACTGGCAAATAAAGAAGAAAACAGAGAAAAAAACAGATATCCCAACATCCTTCCCAATGACCATTCTAGGGTGATTCTGAGCCAACTGGATGGAATTCCCTGTTCAGACTACATCAATGCTTCCTACATAGATGGTTACAAAGAGAAGAATAAATTCATAGCAGCTCAAGGTCCCAAACAGGAAACGGTTAACGACTTCTGGAGAATGGTCTGGGAGCAAAAGTCTGCGACCATCGTCATGTTAACAAACTTGAAAGAAAGGAAAGAGGAAAAGTGCCATCAGTACTGGCCCGACCAAGGCTGCTGGACCTATGGAAACATCCGGGTGTGCGTGGAGGACTGCGTGGTTTTGGTCGACTACACCATCCGGAAGTTCTGCATACAGCCACAGCTCCCCGACGGCTGCAAAGCCCCCAGGCTGGTCTCACAGCTGCACTTCACCAGCTGGCCCGACTTCGGAGTGCCTTTTACCCCCATTGGGATGCTGAAGTTCCTCAAGAAAGTAAAGACGCTCAACCCCGTGCACGCTGGGCCCATCGTGGTCCACTGTAGCGCGGGCGTGGGCCGGACGGGCACCTTCATTGTGATCGATGCCATGATGGCCATGATGCACGCGGAGCAGAAGGTGGATGTGTTTGAATTTGTGTCTCGAATCCGTAATCAGCGCCCTCAGATGGTTCAAACGGATATGCAGTACACGTTCATCTACCAAGCCTTACTCGAGTACTACCTCTACGGGGACACAGAGCTGGACGTGTCCTCCCTGGAGAAGCACCTGCAGACCATGCACGGCACCACCACCCACTTCGACAAGATCGGGCTGGAGGAGGAGTTCAGGAAATTGACAAATGTCCGGATCATGAAGGAGAACATGAGGACGGGCAACTTGCCGGCAAACATGAAGAAGGCCAGGGTCATCCAGATCATCCCGTATGACTTCAACCGAGTGATCCTTTCCATGAAAAGGGGTCAAGAATACACAGACTACATCAACGCATCCTTCATAGACGGCTACCGACAGAAGGACTATTTCATCGCCACCCAGGGGCCACTGGCACACACGGTTGAGGACTTCTGGAGGATGATCTGGGAATGGAAATCCCACACTATCGTGATGCTGACGGAGGTGCAGGAGAGAGAGCAGGATAAATGCTACCAGTATTGGCCAACCGAGGGCTCAGTTACTCATGGAGAAATAACGATTGAGATAAAGAATGATACCCTTTCAGAAGCCATCAGTATACGAGACTTTCTGGTCACTCTCAATCAGCCCCAGGCCCGCCAGGAGGAGCAGGTCCGAGTAGTGCGCCAGTTTCACTTCCACGGCTGGCCTGAGATCGGGATTCCCGCCGAGGGCAAAGGCATGATTGACCTCATCGCAGCCGTGCAGAAGCAGCAGCAGCAGACAGGCAACCACCCCATCACCGTGCACTGCAGTGCCGGAGCTGGGCGAACAGGTACATTCATAGCCCTCAGCAACATTTTGGAGCGAGTAAAAGCCGAGGGACTTTTAGATGTATTTCAAGCTGTGAAGAGTTTACGACTTCAGAGACCACATATGGTGCAAACCCTGGAACAGTATGAATTCTGCTACAAAGTGGTACAAGATTTTATTGATATATTTTCTGATTATGCTAATTTCAAATGAAGATTCCTGCCTTAAAATATTTTTTAATTTAATGGTCAGTATATTTTGTAAAAATCATGTTAATTTATTTCATAGTTGACATTAATATCTTCCCTAATTTCTTTGTATATATTTTGTTATGCCTTAAAGGCCACCTGCTATACAGTTGTTAAATCTTAAATATGCTTTTTAAAAATTGGAATAATGTATTAAGGTCAAATAATATCCCATAAAATATATATTTCTGCTAATATTAGTAAATATCTTAATTTTTCATTAGATTCATATCATTTAATTTCACATATTCAACACCTTTAAATGTTGTAATCTTAATATGCGAAGTGTGCCTCTGCAAGATACTAACACAAAGCTCATGTTAAGAAAACAGTTGAGGACTCAGAAGTCAGTTGAAAATGCACTTTCCTAACAGTGAATTCACAACCCTGAACAGCAGCATTTTTGGAAGGCAAACTGTTCGTGATGGTACAATGTAAATGGGGACTTCTGTAAAGTTCTCAGTTTCGGTCCATGTGGTTTATCTTTACATTTTAAAGATCAAAGAAGTCTTTACAACCTGAATCCAGGTCTAAAACACACTAGAGTAGCTGGTGACTATAAATAATATTTTAAAATGCTGTGTCTACACCATCAAGACTGTGTCTACACTATCTTGGCTGAACGAGAAGAGATGTAAATGCTGGGTGGTCCCGTTGACCCACGGCGTTGGGTACAACAAAACCAGCCATCGGAGTTACACCCCAAAGCACCATTTGCTGTCCAGCTGCCTGTCGTTTGGCCCAGACCACCCTCAGAAAAAAACCAGCTGCCTCTCCCATTCTCCCCTCCCGTTCTGCCACAGCGGCCTGGGCTGGTCCAGTGCTATGCCTGGAGGCTCAACACAAAACTTCCCATCCAAACATTCAGATGAACTGAGCGTCTTACACACGCAGTACAGAGGAGCACACATTAGGATAGAAACAGTAGAATAACCACGGGCAATTAAACTTTAAATTTTCTGAGCAGCATTTTGGTATTTAAACATTTCTTGTAAAAAGCTGAGACAGTTTGTAAGAAAAGAATCCTTAAAATCTAGATTTATACCATTTTTTAAAGTCCCACCTTTCAATGTTTAATAAAACAAAAAGAGAAATCCTTAATCTAAAAGCTAAATTATTTTTGAATGGAAATACTACTGAGACCATTGACACTGGATAACAGTAATGATCCCATTACCAGATAAGATTGACTGACGGGGAAAAAAAAAAAAAAAGAATGGGGTGTGAATGTACCAACACTGAATCTTACAGCAGTTATCTTTCTATGGCCATTAGGTACCTAGCAGATGTGCACAATATAAACAAAAAGATATCTGGCCTACCTTACTACTAAAAGCATTTAACACGTGCATTTTTGGTACTTTTTTTTTTGTTTTCTAAAAGCTACATAAAGGCCTTATTTGACATTTTCACTGATAACTGATCGCACCCTCATGTTGCAGTGTTCGTCCCCTATTCATTAACAGTGTGTGCAAATGCAACCCCAGGACACATTATTGTTCTGATAGATGCTCACAGGGAATCAAGCTTCTCGCCCACTCCACGGCTCTGAGCCCCATCCAAGGGCAAGACTTGGTGCCCAGCTGGAAGGACGAAAGCACACTTTGTGACCGCCATCCTCACCAGCTGCATGCCTGGGCTGCACACTGCTGAACGTGCTCCTCTCTCCTTCTCTGTACAATGATTCAGCATCTCGGCGGAAGAGGAAAATGGAGCTTTTTGAGGCTCGCCAGGTCCCTTTTGTTTTCACCATTAAAATTCCAAACCCAAAGCCTTTGTTTGACTGAAGGAGAAGAGAGGGAAGTAAGCTTCTGTTCAGCACCTGAACCCTAGAAAAAGAGCCAGTTTGCTACGATGAAGGTGACATTTCTCTGGTCATTTATTTGAGAGTTCGAAGTCAAAGTCGAGGGGCACCGGCTTTGGTTCATGTCTAGGAGCCCTCTGTCAGAATCCTTGAAGCCCTTTAATGGTCTAACTGGCATCTCTTGTATCAAGAAGTACCTTTAAGGTAGACCTTTTCAGGGTGCCCTCAGGAAAGGGCCCTGCTCATGTTTTTTTCCTGTCCCCTTAGACCAACCCCAGGTGTCCACTGCAGGGGTTCTGCCTGTTCCCAAACTTTTTCCATTCCAGGAACAAAGGAGAAGCCACTTTCCCCAGGACGCAAGACTCTCCCCTCCACTGTCCGGGACAGCGTTCGCCCTTTAGCGGGGAGGTCATTACAGCCTCATGGCCTCTACCAAGGCCCCAGATCACAGGATCTCCTGGGCCTTGGAGCACCTCACGCTGGGGGAATCAATCCCTGAGGGACTCAGAATCTTCTCCGTGCAACCTGGAAAGTTCATCTCTTGTTTCCTTCAGTCAAAGAAAGTCCATTGTACATAACAAAACAGCCCCCAAACAGCCCAGTGCCGACACCATTGTTCCTTTCACACTTTCCTTTGTTGCATGCAGTTGGGTTCAAATGCCAAATAGTGATTAGAAGACGACCATTCTGATCTGTGTGTGATCTGGTCACTATGTGACTGCCTTTACGGTTTCTCTCCATGTGCTATATGAATGAAGAATGCATACCAGTGTTTTAAAAGGTATTTTTATGTGTTTTTAAACACTTTTTTAAATGAGCCTGACACCTGTGTTTCAGCATTTGGAGACATCCCCATGTTATTCTTTTAAGTGTATAATTACTGATACTTTTTTGTTTGTTTGTTTAACTAAGTTGTGTTTAACTTATGTGCAGTCTTTATAATGTATGTATGTTATTACAGTTTCAACTATCATATTTTCTTTGATTACATTTATAATTTGATCTTGCTCTGATTATAATGCCAGTGAATGTTGCTGAACTCTTTGTATATGCAAATTGCAAGATTTAAACCATTCTGATGCAAGGATAAACCTTTACTTTGACTACCAGCCTGTGTTTTTGTCTTTAAATCTCTTAATTTCATTCCTCTGCAAA
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 57526, GC content failed 3887, low tm 24767, high tm 17297, high any compl 7, high end compl 38, high repeat similarity 42, long poly-x seq 179, ok 11309
PRIMER_RIGHT_EXPLAIN=considered 57585, GC content failed 4010, low tm 25269, high tm 16658, high end compl 12, high repeat similarity 8, long poly-x seq 174, ok 11454
PRIMER_PAIR_EXPLAIN=considered 1001, unacceptable product size 924, high end compl 7, ok 70
PRIMER_PAIR_PENALTY=0.0527
PRIMER_LEFT_PENALTY=0.037129
PRIMER_RIGHT_PENALTY=0.015613
PRIMER_LEFT_SEQUENCE=GGCTACCGACAGAAGGACTATTT
PRIMER_RIGHT_SEQUENCE=GGCTTCTGAAAGGGTATCATTCT
PRIMER_LEFT=1369,23
PRIMER_RIGHT=1593,23
PRIMER_LEFT_TM=60.037
PRIMER_RIGHT_TM=59.984
PRIMER_LEFT_GC_PERCENT=47.826
PRIMER_RIGHT_GC_PERCENT=43.478
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_MISPRIMING_SCORE=11.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_END_STABILITY=6.2000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=225
PRIMER_PAIR_PENALTY_1=0.0585
PRIMER_LEFT_1_PENALTY=0.036623
PRIMER_RIGHT_1_PENALTY=0.021925
PRIMER_LEFT_1_SEQUENCE=GAAGTGTGCCTCTGCAAGATACT
PRIMER_RIGHT_1_SEQUENCE=AAAGTTTAATTGCCCGTGGTTAT
PRIMER_LEFT_1=2325,23
PRIMER_RIGHT_1=2994,23
PRIMER_LEFT_1_TM=59.963
PRIMER_RIGHT_1_TM=60.022
PRIMER_LEFT_1_GC_PERCENT=47.826
PRIMER_RIGHT_1_GC_PERCENT=34.783
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, MER34 a consensus
PRIMER_PAIR_1_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_1_END_STABILITY=5.3000
PRIMER_RIGHT_1_END_STABILITY=5.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=670
PRIMER_PAIR_PENALTY_2=0.0668
PRIMER_LEFT_2_PENALTY=0.044904
PRIMER_RIGHT_2_PENALTY=0.021925
PRIMER_LEFT_2_SEQUENCE=TGTGCCTCTGCAAGATACTAACA
PRIMER_RIGHT_2_SEQUENCE=AAAGTTTAATTGCCCGTGGTTAT
PRIMER_LEFT_2=2329,23
PRIMER_RIGHT_2=2994,23
PRIMER_LEFT_2_TM=59.955
PRIMER_RIGHT_2_TM=60.022
PRIMER_LEFT_2_GC_PERCENT=43.478
PRIMER_RIGHT_2_GC_PERCENT=34.783
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_MISPRIMING_SCORE=11.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_2_MISPRIMING_SCORE=12.00, MER34 a consensus
PRIMER_PAIR_2_MISPRIMING_SCORE=20.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_2_END_STABILITY=6.0000
PRIMER_RIGHT_2_END_STABILITY=5.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=666
PRIMER_PAIR_PENALTY_3=0.0668
PRIMER_LEFT_3_PENALTY=0.044904
PRIMER_RIGHT_3_PENALTY=0.021925
PRIMER_LEFT_3_SEQUENCE=TGCCTCTGCAAGATACTAACACA
PRIMER_RIGHT_3_SEQUENCE=AAAGTTTAATTGCCCGTGGTTAT
PRIMER_LEFT_3=2331,23
PRIMER_RIGHT_3=2994,23
PRIMER_LEFT_3_TM=59.955
PRIMER_RIGHT_3_TM=60.022
PRIMER_LEFT_3_GC_PERCENT=43.478
PRIMER_RIGHT_3_GC_PERCENT=34.783
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, MER34 a consensus
PRIMER_PAIR_3_MISPRIMING_SCORE=21.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=5.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=664
PRIMER_PAIR_PENALTY_4=0.0738
PRIMER_LEFT_4_PENALTY=0.037129
PRIMER_RIGHT_4_PENALTY=0.036623
PRIMER_LEFT_4_SEQUENCE=GGCTACCGACAGAAGGACTATTT
PRIMER_RIGHT_4_SEQUENCE=AGTATCTTGCAGAGGCACACTTC
PRIMER_LEFT_4=1369,23
PRIMER_RIGHT_4=2347,23
PRIMER_LEFT_4_TM=60.037
PRIMER_RIGHT_4_TM=59.963
PRIMER_LEFT_4_GC_PERCENT=47.826
PRIMER_RIGHT_4_GC_PERCENT=47.826
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_MISPRIMING_SCORE=11.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_4_MISPRIMING_SCORE=11.00, MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_PAIR_4_MISPRIMING_SCORE=20.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_4_END_STABILITY=6.2000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=979
PRIMER_PAIR_PENALTY_5=0.0779
PRIMER_LEFT_5_PENALTY=0.041227
PRIMER_RIGHT_5_PENALTY=0.036623
PRIMER_LEFT_5_SEQUENCE=GTACATTCATAGCCCTCAGCAAC
PRIMER_RIGHT_5_SEQUENCE=AGTATCTTGCAGAGGCACACTTC
PRIMER_LEFT_5=1817,23
PRIMER_RIGHT_5=2347,23
PRIMER_LEFT_5_TM=60.041
PRIMER_RIGHT_5_TM=59.963
PRIMER_LEFT_5_GC_PERCENT=47.826
PRIMER_RIGHT_5_GC_PERCENT=47.826
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=2.00
PRIMER_LEFT_5_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_5_MISPRIMING_SCORE=11.00, MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_PAIR_5_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_5_END_STABILITY=8.2000
PRIMER_RIGHT_5_END_STABILITY=6.4000
PRIMER_PAIR_5_COMPL_ANY=7.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_5=531
PRIMER_PAIR_PENALTY_6=0.0820
PRIMER_LEFT_6_PENALTY=0.037129
PRIMER_RIGHT_6_PENALTY=0.044904
PRIMER_LEFT_6_SEQUENCE=GGCTACCGACAGAAGGACTATTT
PRIMER_RIGHT_6_SEQUENCE=TGTTAGTATCTTGCAGAGGCACA
PRIMER_LEFT_6=1369,23
PRIMER_RIGHT_6=2351,23
PRIMER_LEFT_6_TM=60.037
PRIMER_RIGHT_6_TM=59.955
PRIMER_LEFT_6_GC_PERCENT=47.826
PRIMER_RIGHT_6_GC_PERCENT=43.478
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=2.00
PRIMER_LEFT_6_MISPRIMING_SCORE=11.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_6_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_6_MISPRIMING_SCORE=22.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_6_END_STABILITY=6.2000
PRIMER_RIGHT_6_END_STABILITY=8.2000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_6=983
PRIMER_PAIR_PENALTY_7=0.0820
PRIMER_LEFT_7_PENALTY=0.037129
PRIMER_RIGHT_7_PENALTY=0.044904
PRIMER_LEFT_7_SEQUENCE=GGCTACCGACAGAAGGACTATTT
PRIMER_RIGHT_7_SEQUENCE=TGTGTTAGTATCTTGCAGAGGCA
PRIMER_LEFT_7=1369,23
PRIMER_RIGHT_7=2353,23
PRIMER_LEFT_7_TM=60.037
PRIMER_RIGHT_7_TM=59.955
PRIMER_LEFT_7_GC_PERCENT=47.826
PRIMER_RIGHT_7_GC_PERCENT=43.478
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=2.00
PRIMER_LEFT_7_MISPRIMING_SCORE=11.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_7_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_7_MISPRIMING_SCORE=21.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_7_END_STABILITY=6.2000
PRIMER_RIGHT_7_END_STABILITY=9.7000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_7=985
PRIMER_PAIR_PENALTY_8=0.0903
PRIMER_LEFT_8_PENALTY=0.074695
PRIMER_RIGHT_8_PENALTY=0.015613
PRIMER_LEFT_8_SEQUENCE=ACAGACTACATCAACGCATCCTT
PRIMER_RIGHT_8_SEQUENCE=GGCTTCTGAAAGGGTATCATTCT
PRIMER_LEFT_8=1339,23
PRIMER_RIGHT_8=1593,23
PRIMER_LEFT_8_TM=60.075
PRIMER_RIGHT_8_TM=59.984
PRIMER_LEFT_8_GC_PERCENT=43.478
PRIMER_RIGHT_8_GC_PERCENT=43.478
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=5.00
PRIMER_LEFT_8_SELF_END=2.00
PRIMER_RIGHT_8_SELF_END=2.00
PRIMER_LEFT_8_MISPRIMING_SCORE=12.00, reverse L1PA7 3'-end of L1 repeat (subfamily L1PA7) - a consensus sequence
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_8_MISPRIMING_SCORE=22.00, reverse Tigger1 Autonomous DNA transposon
PRIMER_LEFT_8_END_STABILITY=8.2000
PRIMER_RIGHT_8_END_STABILITY=6.6000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_8=255
PRIMER_PAIR_PENALTY_9=0.0978
PRIMER_LEFT_9_PENALTY=0.082173
PRIMER_RIGHT_9_PENALTY=0.015613
PRIMER_LEFT_9_SEQUENCE=ACTCGAGTACTACCTCTACGGGG
PRIMER_RIGHT_9_SEQUENCE=GGCTTCTGAAAGGGTATCATTCT
PRIMER_LEFT_9=1077,23
PRIMER_RIGHT_9=1593,23
PRIMER_LEFT_9_TM=60.082
PRIMER_RIGHT_9_TM=59.984
PRIMER_LEFT_9_GC_PERCENT=56.522
PRIMER_RIGHT_9_GC_PERCENT=43.478
PRIMER_LEFT_9_SELF_ANY=8.00
PRIMER_RIGHT_9_SELF_ANY=5.00
PRIMER_LEFT_9_SELF_END=2.00
PRIMER_RIGHT_9_SELF_END=2.00
PRIMER_LEFT_9_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_9_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_9_END_STABILITY=12.9000
PRIMER_RIGHT_9_END_STABILITY=6.6000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_9=517
PRIMER_PAIR_PENALTY_10=0.1041
PRIMER_LEFT_10_PENALTY=0.082173
PRIMER_RIGHT_10_PENALTY=0.021925
PRIMER_LEFT_10_SEQUENCE=TTCAAATGAAGATTCCTGCCTTA
PRIMER_RIGHT_10_SEQUENCE=AAAGTTTAATTGCCCGTGGTTAT
PRIMER_LEFT_10=1999,23
PRIMER_RIGHT_10=2994,23
PRIMER_LEFT_10_TM=60.082
PRIMER_RIGHT_10_TM=60.022
PRIMER_LEFT_10_GC_PERCENT=34.783
PRIMER_RIGHT_10_GC_PERCENT=34.783
PRIMER_LEFT_10_SELF_ANY=6.00
PRIMER_RIGHT_10_SELF_ANY=5.00
PRIMER_LEFT_10_SELF_END=2.00
PRIMER_RIGHT_10_SELF_END=2.00
PRIMER_LEFT_10_MISPRIMING_SCORE=12.00, MER21 a consensus
PRIMER_RIGHT_10_MISPRIMING_SCORE=12.00, MER34 a consensus
PRIMER_PAIR_10_MISPRIMING_SCORE=20.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_10_END_STABILITY=7.5000
PRIMER_RIGHT_10_END_STABILITY=5.6000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_10=996
PRIMER_PAIR_PENALTY_11=0.1163
PRIMER_LEFT_11_PENALTY=0.094334
PRIMER_RIGHT_11_PENALTY=0.021925
PRIMER_LEFT_11_SEQUENCE=GGCTGAACGAGAAGAGATGTAAA
PRIMER_RIGHT_11_SEQUENCE=AAAGTTTAATTGCCCGTGGTTAT
PRIMER_LEFT_11=2659,23
PRIMER_RIGHT_11=2994,23
PRIMER_LEFT_11_TM=59.906
PRIMER_RIGHT_11_TM=60.022
PRIMER_LEFT_11_GC_PERCENT=43.478
PRIMER_RIGHT_11_GC_PERCENT=34.783
PRIMER_LEFT_11_SELF_ANY=2.00
PRIMER_RIGHT_11_SELF_ANY=5.00
PRIMER_LEFT_11_SELF_END=2.00
PRIMER_RIGHT_11_SELF_END=2.00
PRIMER_LEFT_11_MISPRIMING_SCORE=12.00, L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, MER34 a consensus
PRIMER_PAIR_11_MISPRIMING_SCORE=21.00, MER34 a consensus
PRIMER_LEFT_11_END_STABILITY=6.0000
PRIMER_RIGHT_11_END_STABILITY=5.6000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_11=336
PRIMER_PAIR_PENALTY_12=0.1188
PRIMER_LEFT_12_PENALTY=0.082173
PRIMER_RIGHT_12_PENALTY=0.036623
PRIMER_LEFT_12_SEQUENCE=TTCAAATGAAGATTCCTGCCTTA
PRIMER_RIGHT_12_SEQUENCE=AGTATCTTGCAGAGGCACACTTC
PRIMER_LEFT_12=1999,23
PRIMER_RIGHT_12=2347,23
PRIMER_LEFT_12_TM=60.082
PRIMER_RIGHT_12_TM=59.963
PRIMER_LEFT_12_GC_PERCENT=34.783
PRIMER_RIGHT_12_GC_PERCENT=47.826
PRIMER_LEFT_12_SELF_ANY=6.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=2.00
PRIMER_RIGHT_12_SELF_END=2.00
PRIMER_LEFT_12_MISPRIMING_SCORE=12.00, MER21 a consensus
PRIMER_RIGHT_12_MISPRIMING_SCORE=11.00, MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_PAIR_12_MISPRIMING_SCORE=20.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_12_END_STABILITY=7.5000
PRIMER_RIGHT_12_END_STABILITY=6.4000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_12=349
PRIMER_PAIR_PENALTY_13=0.1193
PRIMER_LEFT_13_PENALTY=0.082173
PRIMER_RIGHT_13_PENALTY=0.037129
PRIMER_LEFT_13_SEQUENCE=ACTCGAGTACTACCTCTACGGGG
PRIMER_RIGHT_13_SEQUENCE=AAATAGTCCTTCTGTCGGTAGCC
PRIMER_LEFT_13=1077,23
PRIMER_RIGHT_13=1391,23
PRIMER_LEFT_13_TM=60.082
PRIMER_RIGHT_13_TM=60.037
PRIMER_LEFT_13_GC_PERCENT=56.522
PRIMER_RIGHT_13_GC_PERCENT=47.826
PRIMER_LEFT_13_SELF_ANY=8.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=2.00
PRIMER_RIGHT_13_SELF_END=2.00
PRIMER_LEFT_13_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_13_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_13_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_13_END_STABILITY=12.9000
PRIMER_RIGHT_13_END_STABILITY=8.7000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_13=315
PRIMER_PAIR_PENALTY_14=0.1225
PRIMER_LEFT_14_PENALTY=0.033905
PRIMER_RIGHT_14_PENALTY=0.088604
PRIMER_LEFT_14_SEQUENCE=TATAATGCCAGTGAATGTTGCTG
PRIMER_RIGHT_14_SEQUENCE=GACAAAAACACAGGCTGGTAGTC
PRIMER_LEFT_14=4861,23
PRIMER_RIGHT_14=4970,23
PRIMER_LEFT_14_TM=60.034
PRIMER_RIGHT_14_TM=60.089
PRIMER_LEFT_14_GC_PERCENT=39.130
PRIMER_RIGHT_14_GC_PERCENT=47.826
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=5.00
PRIMER_LEFT_14_SELF_END=2.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_MISPRIMING_SCORE=11.00, MER1A Nonautonomous DNA transposon
PRIMER_RIGHT_14_MISPRIMING_SCORE=12.00, reverse LTR2 LTR from human endogenous retrovirus (4-14), 3' long terminal repeat
PRIMER_PAIR_14_MISPRIMING_SCORE=21.00, MER4C a consensus
PRIMER_LEFT_14_END_STABILITY=8.5000
PRIMER_RIGHT_14_END_STABILITY=5.4000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_14=110
PRIMER_PAIR_PENALTY_15=0.1233
PRIMER_LEFT_15_PENALTY=0.034654
PRIMER_RIGHT_15_PENALTY=0.088604
PRIMER_LEFT_15_SEQUENCE=TACGGTTTCTCTCCATGTGCTAT
PRIMER_RIGHT_15_SEQUENCE=GACAAAAACACAGGCTGGTAGTC
PRIMER_LEFT_15=4572,23
PRIMER_RIGHT_15=4970,23
PRIMER_LEFT_15_TM=60.035
PRIMER_RIGHT_15_TM=60.089
PRIMER_LEFT_15_GC_PERCENT=43.478
PRIMER_RIGHT_15_GC_PERCENT=47.826
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=5.00
PRIMER_LEFT_15_SELF_END=2.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_MISPRIMING_SCORE=12.00, LTR5 LTR from human endogenous retrovirus 5' LTR, clone HERV-K18
PRIMER_RIGHT_15_MISPRIMING_SCORE=12.00, reverse LTR2 LTR from human endogenous retrovirus (4-14), 3' long terminal repeat
PRIMER_PAIR_15_MISPRIMING_SCORE=23.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_15_END_STABILITY=7.1000
PRIMER_RIGHT_15_END_STABILITY=5.4000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_15=399
PRIMER_PAIR_PENALTY_16=0.1271
PRIMER_LEFT_16_PENALTY=0.082173
PRIMER_RIGHT_16_PENALTY=0.044904
PRIMER_LEFT_16_SEQUENCE=TTCAAATGAAGATTCCTGCCTTA
PRIMER_RIGHT_16_SEQUENCE=TGTTAGTATCTTGCAGAGGCACA
PRIMER_LEFT_16=1999,23
PRIMER_RIGHT_16=2351,23
PRIMER_LEFT_16_TM=60.082
PRIMER_RIGHT_16_TM=59.955
PRIMER_LEFT_16_GC_PERCENT=34.783
PRIMER_RIGHT_16_GC_PERCENT=43.478
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=2.00
PRIMER_RIGHT_16_SELF_END=2.00
PRIMER_LEFT_16_MISPRIMING_SCORE=12.00, MER21 a consensus
PRIMER_RIGHT_16_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_16_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_16_END_STABILITY=7.5000
PRIMER_RIGHT_16_END_STABILITY=8.2000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_16=353
PRIMER_PAIR_PENALTY_17=0.1271
PRIMER_LEFT_17_PENALTY=0.082173
PRIMER_RIGHT_17_PENALTY=0.044904
PRIMER_LEFT_17_SEQUENCE=TTCAAATGAAGATTCCTGCCTTA
PRIMER_RIGHT_17_SEQUENCE=TGTGTTAGTATCTTGCAGAGGCA
PRIMER_LEFT_17=1999,23
PRIMER_RIGHT_17=2353,23
PRIMER_LEFT_17_TM=60.082
PRIMER_RIGHT_17_TM=59.955
PRIMER_LEFT_17_GC_PERCENT=34.783
PRIMER_RIGHT_17_GC_PERCENT=43.478
PRIMER_LEFT_17_SELF_ANY=6.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=2.00
PRIMER_RIGHT_17_SELF_END=2.00
PRIMER_LEFT_17_MISPRIMING_SCORE=12.00, MER21 a consensus
PRIMER_RIGHT_17_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_17_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_17_END_STABILITY=7.5000
PRIMER_RIGHT_17_END_STABILITY=9.7000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_17=355
PRIMER_PAIR_PENALTY_18=0.1279
PRIMER_LEFT_18_PENALTY=0.109631
PRIMER_RIGHT_18_PENALTY=0.018314
PRIMER_LEFT_18_SEQUENCE=TCTGATAGATGCTCACAGGGAAT
PRIMER_RIGHT_18_SEQUENCE=ATTAAAGGGCTTCAAGGATTCTG
PRIMER_LEFT_18=3543,23
PRIMER_RIGHT_18=4010,23
PRIMER_LEFT_18_TM=60.110
PRIMER_RIGHT_18_TM=59.982
PRIMER_LEFT_18_GC_PERCENT=43.478
PRIMER_RIGHT_18_GC_PERCENT=39.130
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=6.00
PRIMER_LEFT_18_SELF_END=2.00
PRIMER_RIGHT_18_SELF_END=2.00
PRIMER_LEFT_18_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_18_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_18_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_18_END_STABILITY=8.1000
PRIMER_RIGHT_18_END_STABILITY=7.0000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_18=468
PRIMER_PAIR_PENALTY_19=0.1279
PRIMER_LEFT_19_PENALTY=0.109631
PRIMER_RIGHT_19_PENALTY=0.018314
PRIMER_LEFT_19_SEQUENCE=CTGATAGATGCTCACAGGGAATC
PRIMER_RIGHT_19_SEQUENCE=ATTAAAGGGCTTCAAGGATTCTG
PRIMER_LEFT_19=3544,23
PRIMER_RIGHT_19=4010,23
PRIMER_LEFT_19_TM=60.110
PRIMER_RIGHT_19_TM=59.982
PRIMER_LEFT_19_GC_PERCENT=47.826
PRIMER_RIGHT_19_GC_PERCENT=39.130
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=3.00
PRIMER_RIGHT_19_SELF_END=2.00
PRIMER_LEFT_19_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_19_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_19_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_19_END_STABILITY=6.6000
PRIMER_RIGHT_19_END_STABILITY=7.0000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_19=467
=