PRIMER_SEQUENCE_ID=NM_152511
SEQUENCE=ACATGCGCACAGAGCCCGGGCGGCTACGGAAGCGGTGAGACTGTCTCTCGGCTGCAGCCCTGGTGCGACCCGGCCCGTTGCCGTAGAGATGGGCAGGGCTGGATGGAGTGGGGTGCGGTGAGCTGAGCTGACCCTGCTTCGCCACGGGGACTGCAGTGACCCCGGCTTGCCGGCAGGGCGGGTAACAGGTTGAGCCAGGGTGGGGCTGCTCAGGGGCGTGGAGCCGAGGCCAGGATTTCTCTGAAGACCCGGCACAGGCTATTCCTTTCTGCGACGAGCCCATTGCTATGGAAACCAAAGCGTTAGGCCAGCGGGGATTGAGGCTGCGGGATCATGACGGGTCTCTCTCCCGAAGAACCTTGCCTAAGGCTTCCCCAAGCGGCTACTTCCTGAGCGAACCCGCCCACCCGCCTGAAGGAGAGAGTTTTCCATGGACACAGCCTAGCAGAAAGACGCAGCCTTCGTGCTTCGCTGACTGCTGACCACTGACCCACCGCCTTGATGGCAGCACCCTCGTGTGCCTTCCCAGTTCAGTTCCGGCAGCCCTCAGTCAGCGGCCTCTCGCAGATAACCAAAAGCCTGTATATCAGCAATGGTGTGGCCGCCAACAACAAGCTCATGCTGTCTAGCAACCAGATCACCATGGTCATCAATGTCTCAGTGGAGGTAGTGAACACCTTGTATGAGGATATCCAGTACATGCAGGTACCTGTGGCTGACTCCCCTAACTCACGTCTCTGTGACTTCTTTGACCCTATTGCTGACCATATCCACAGCGTGGAGATGAAGCAGGGCCGTACTTTGCTGCACTGTGCTGCTGGTGTGAGCCGCTCAGCTGCCCTGTGCCTCGCCTACCTCATGAAGTACCACGCCATGTCCCTGCTGGACGCCCACACGTGGACCAAGTCATGCCGGCCCATCATCCGACCCAACAGCGGCTTTTGGGAGCAGCTCATCCACTATGAGTTCCAATTGTTTGGCAAGAACACTGTGCACATGGTCAGTTCCCCAGTGGGAATGATCCCTGACATCTATGAGAAGGAAGTCCGTTTGATGATTCCACTGTGAGCCATCCCACGAGCCCCTGCATTGGAGTCAGAGGTACAGATCTATTGTTGATCTTACACCAAGATCCAAACTTGAACATTCTACTTTTGTTGATACAGAAAAAAACAGATGATGCCTTTTATGAGCACAAAAAAGAGTTGCTGTAGCTTTTAACTTTATAATCCATTTTTTTTAAGATTAAACTAATTGTGAGATGGTGAAGATAAATTTTCTGCCCTGTGAGTGACACTGACCAGGGGATAGGTTGAGGCAGATGGTGCCCAGAAGAAAGAGGGCAGCACCCATTGCACATGGCAGGCCTGGAATCCTGCAGCCCTCCCCAAAAACAGATTGACCCAGGAATGAATCTGCTACAATTCCACCTCATTCTTTCACACCCAGAGCCAGAGCCTTAAGCATCATGGTACCTCTTGCGCTTCTTACAGTGAGTGCCAATCTACTAGATAAATGGCCTTCAGGCAGTGCTCCAGGAATCCTGGGGGGTCCCAGGCCACCTCTGCTTCCACCTTCACTATAAGTGGCCCAGTTCTGGTTTTCTAGATTGTACTTATGCATAAGATAGTTTTTAAAGAAAGCATTCCACTGTGTAAATTTTTTTTTGTCTTCTTTTGAAACTGTCCTGCTCTGTCACCCATCCTGGAGTGCAGTAGTGTGATCATGGCTCACTGTAGCCACAACCTCTCAGGCTCAAGTGATCCTCTTACCTTAGCCTCCTGCGTGGCTGGGACTACAGATGTTTGCCACCATGCCCGCCTCATTTTTTTTCTTTTTTTATAGAGATGAGATTTTACTATGTCGCCCAGACTGGTCTCAAACTCCTGGCCTCAAGCAATCCTCACGCCTCAGCCTCCCAAAGTGTTGATGAGCCACTGTACCCCGATACCACTGTATAAAAAATTTAAAAAAAATTGTGAGTGCTATTGTACATGGCAAAGTTTCAAAGAGCTCTTGGCCATCCCTCACCAAACTTTGGCAAAAGATGGTGTTAGTCCCCTTCTCCAGGGCACATGAGAAAAACAGGCCTAACATCAGGTCTCAGCGGTTCCTCTCACAGGCCTTGGCTTGGAAGGCTGGAGCTTCGGGCCAAGGCCCAGCCCTGTCACCTCCTCTTCATGTGATGTGGACCCCTGGGAGCATCAGTGTCCTCATCTGTCCAATGACAGCCCTTTTCTCACAGAGTTATTGTGAAGATTCAACAATTTCTTGGCATAGTGCCTGGCACATGGTGTGCGCTTAGCGCAAATGGCAGCTCTCATCATGAATGATAGACTCTTTCACCCTGCTGGTCCCAGGTCGGCACACACATCCTCATAATGGCATTTCTCCTTTCTGTGCACAGCACTTTATTGTTACAAAGTACTCTTCCAAAAAGTTACCCTGTGTGTAAAAAAAAAAA
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 27381, GC content failed 845, low tm 9215, high tm 11672, high any compl 2, high end compl 21, high repeat similarity 20, long poly-x seq 138, ok 5468
PRIMER_RIGHT_EXPLAIN=considered 27354, GC content failed 793, low tm 9716, high tm 11095, high any compl 2, high end compl 8, high repeat similarity 9, long poly-x seq 150, ok 5581
PRIMER_PAIR_EXPLAIN=considered 268, unacceptable product size 222, high end compl 2, ok 44
PRIMER_PAIR_PENALTY=0.0318
PRIMER_LEFT_PENALTY=0.014220
PRIMER_RIGHT_PENALTY=0.017590
PRIMER_LEFT_SEQUENCE=TGCTACAATTCCACCTCATTCTT
PRIMER_RIGHT_SEQUENCE=AATCTAGAAAACCAGAACTGGGC
PRIMER_LEFT=1416,23
PRIMER_RIGHT=1610,23
PRIMER_LEFT_TM=60.014
PRIMER_RIGHT_TM=60.018
PRIMER_LEFT_GC_PERCENT=39.130
PRIMER_RIGHT_GC_PERCENT=43.478
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_MISPRIMING_SCORE=11.00, MER22 Human SSTI moderately repetitive DNA sequence family
PRIMER_RIGHT_MISPRIMING_SCORE=12.00, MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_PAIR_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=11.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=195
PRIMER_PAIR_PENALTY_1=0.0402
PRIMER_LEFT_1_PENALTY=0.014220
PRIMER_RIGHT_1_PENALTY=0.026007
PRIMER_LEFT_1_SEQUENCE=TGCTACAATTCCACCTCATTCTT
PRIMER_RIGHT_1_SEQUENCE=TAAGAGGATCACTTGAGCCTGAG
PRIMER_LEFT_1=1416,23
PRIMER_RIGHT_1=1770,23
PRIMER_LEFT_1_TM=60.014
PRIMER_RIGHT_1_TM=60.026
PRIMER_LEFT_1_GC_PERCENT=39.130
PRIMER_RIGHT_1_GC_PERCENT=47.826
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_MISPRIMING_SCORE=11.00, MER22 Human SSTI moderately repetitive DNA sequence family
PRIMER_RIGHT_1_MISPRIMING_SCORE=11.00, SVA Composite retroposon
PRIMER_PAIR_1_MISPRIMING_SCORE=21.00, MER22 Human SSTI moderately repetitive DNA sequence family
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=355
PRIMER_PAIR_PENALTY_2=0.0436
PRIMER_LEFT_2_PENALTY=0.017590
PRIMER_RIGHT_2_PENALTY=0.026007
PRIMER_LEFT_2_SEQUENCE=GCCCAGTTCTGGTTTTCTAGATT
PRIMER_RIGHT_2_SEQUENCE=TAAGAGGATCACTTGAGCCTGAG
PRIMER_LEFT_2=1588,23
PRIMER_RIGHT_2=1770,23
PRIMER_LEFT_2_TM=60.018
PRIMER_RIGHT_2_TM=60.026
PRIMER_LEFT_2_GC_PERCENT=43.478
PRIMER_RIGHT_2_GC_PERCENT=47.826
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_RIGHT_2_MISPRIMING_SCORE=11.00, SVA Composite retroposon
PRIMER_PAIR_2_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=183
PRIMER_PAIR_PENALTY_3=0.0626
PRIMER_LEFT_3_PENALTY=0.014220
PRIMER_RIGHT_3_PENALTY=0.048411
PRIMER_LEFT_3_SEQUENCE=TGCTACAATTCCACCTCATTCTT
PRIMER_RIGHT_3_SEQUENCE=TGTTAGGCCTGTTTTTCTCATGT
PRIMER_LEFT_3=1416,23
PRIMER_RIGHT_3=2095,23
PRIMER_LEFT_3_TM=60.014
PRIMER_RIGHT_3_TM=60.048
PRIMER_LEFT_3_GC_PERCENT=39.130
PRIMER_RIGHT_3_GC_PERCENT=39.130
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_MISPRIMING_SCORE=11.00, MER22 Human SSTI moderately repetitive DNA sequence family
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_3_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=680
PRIMER_PAIR_PENALTY_4=0.0660
PRIMER_LEFT_4_PENALTY=0.017590
PRIMER_RIGHT_4_PENALTY=0.048411
PRIMER_LEFT_4_SEQUENCE=GCCCAGTTCTGGTTTTCTAGATT
PRIMER_RIGHT_4_SEQUENCE=TGTTAGGCCTGTTTTTCTCATGT
PRIMER_LEFT_4=1588,23
PRIMER_RIGHT_4=2095,23
PRIMER_LEFT_4_TM=60.018
PRIMER_RIGHT_4_TM=60.048
PRIMER_LEFT_4_GC_PERCENT=43.478
PRIMER_RIGHT_4_GC_PERCENT=39.130
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_4_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=508
PRIMER_PAIR_PENALTY_5=0.0687
PRIMER_LEFT_5_PENALTY=0.017590
PRIMER_RIGHT_5_PENALTY=0.051149
PRIMER_LEFT_5_SEQUENCE=GCCCAGTTCTGGTTTTCTAGATT
PRIMER_RIGHT_5_SEQUENCE=CACACAGGGTAACTTTTTGGAAG
PRIMER_LEFT_5=1588,23
PRIMER_RIGHT_5=2451,23
PRIMER_LEFT_5_TM=60.018
PRIMER_RIGHT_5_TM=59.949
PRIMER_LEFT_5_GC_PERCENT=43.478
PRIMER_RIGHT_5_GC_PERCENT=43.478
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=2.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_MISPRIMING_SCORE=12.00, MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_RIGHT_5_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_5_MISPRIMING_SCORE=22.00, reverse L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_LEFT_5_END_STABILITY=6.6000
PRIMER_RIGHT_5_END_STABILITY=8.2000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_5=864
PRIMER_PAIR_PENALTY_6=0.0744
PRIMER_LEFT_6_PENALTY=0.026007
PRIMER_RIGHT_6_PENALTY=0.048411
PRIMER_LEFT_6_SEQUENCE=CTCAGGCTCAAGTGATCCTCTTA
PRIMER_RIGHT_6_SEQUENCE=TGTTAGGCCTGTTTTTCTCATGT
PRIMER_LEFT_6=1748,23
PRIMER_RIGHT_6=2095,23
PRIMER_LEFT_6_TM=60.026
PRIMER_RIGHT_6_TM=60.048
PRIMER_LEFT_6_GC_PERCENT=47.826
PRIMER_RIGHT_6_GC_PERCENT=39.130
PRIMER_LEFT_6_SELF_ANY=8.00
PRIMER_RIGHT_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=2.00
PRIMER_RIGHT_6_SELF_END=2.00
PRIMER_LEFT_6_MISPRIMING_SCORE=12.00, MARINER1 Human mariner
PRIMER_RIGHT_6_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_6_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_6_END_STABILITY=6.0000
PRIMER_RIGHT_6_END_STABILITY=6.6000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_6=348
PRIMER_PAIR_PENALTY_7=0.0772
PRIMER_LEFT_7_PENALTY=0.026007
PRIMER_RIGHT_7_PENALTY=0.051149
PRIMER_LEFT_7_SEQUENCE=CTCAGGCTCAAGTGATCCTCTTA
PRIMER_RIGHT_7_SEQUENCE=CACACAGGGTAACTTTTTGGAAG
PRIMER_LEFT_7=1748,23
PRIMER_RIGHT_7=2451,23
PRIMER_LEFT_7_TM=60.026
PRIMER_RIGHT_7_TM=59.949
PRIMER_LEFT_7_GC_PERCENT=47.826
PRIMER_RIGHT_7_GC_PERCENT=43.478
PRIMER_LEFT_7_SELF_ANY=8.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=2.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_MISPRIMING_SCORE=12.00, MARINER1 Human mariner
PRIMER_RIGHT_7_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_7_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_7_END_STABILITY=6.0000
PRIMER_RIGHT_7_END_STABILITY=8.2000
PRIMER_PAIR_7_COMPL_ANY=6.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_7=704
PRIMER_PAIR_PENALTY_8=0.0996
PRIMER_LEFT_8_PENALTY=0.048411
PRIMER_RIGHT_8_PENALTY=0.051149
PRIMER_LEFT_8_SEQUENCE=ACATGAGAAAAACAGGCCTAACA
PRIMER_RIGHT_8_SEQUENCE=CACACAGGGTAACTTTTTGGAAG
PRIMER_LEFT_8=2073,23
PRIMER_RIGHT_8=2451,23
PRIMER_LEFT_8_TM=60.048
PRIMER_RIGHT_8_TM=59.949
PRIMER_LEFT_8_GC_PERCENT=39.130
PRIMER_RIGHT_8_GC_PERCENT=43.478
PRIMER_LEFT_8_SELF_ANY=6.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_8_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_8_END_STABILITY=6.0000
PRIMER_RIGHT_8_END_STABILITY=8.2000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_8=379
PRIMER_PAIR_PENALTY_9=0.1267
PRIMER_LEFT_9_PENALTY=0.014220
PRIMER_RIGHT_9_PENALTY=0.112479
PRIMER_LEFT_9_SEQUENCE=TGCTACAATTCCACCTCATTCTT
PRIMER_RIGHT_9_SEQUENCE=GAGAAGGGGACTAACACCATCTT
PRIMER_LEFT_9=1416,23
PRIMER_RIGHT_9=2066,23
PRIMER_LEFT_9_TM=60.014
PRIMER_RIGHT_9_TM=59.888
PRIMER_LEFT_9_GC_PERCENT=39.130
PRIMER_RIGHT_9_GC_PERCENT=47.826
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=2.00
PRIMER_LEFT_9_MISPRIMING_SCORE=11.00, MER22 Human SSTI moderately repetitive DNA sequence family
PRIMER_RIGHT_9_MISPRIMING_SCORE=11.00, reverse L1PA2 3'-end of L1 repeat (subfamily L1PA2) - a consensus sequence
PRIMER_PAIR_9_MISPRIMING_SCORE=20.00, MER22 Human SSTI moderately repetitive DNA sequence family
PRIMER_LEFT_9_END_STABILITY=7.0000
PRIMER_RIGHT_9_END_STABILITY=6.6000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_9=651
PRIMER_PAIR_PENALTY_10=0.1301
PRIMER_LEFT_10_PENALTY=0.017590
PRIMER_RIGHT_10_PENALTY=0.112479
PRIMER_LEFT_10_SEQUENCE=GCCCAGTTCTGGTTTTCTAGATT
PRIMER_RIGHT_10_SEQUENCE=GAGAAGGGGACTAACACCATCTT
PRIMER_LEFT_10=1588,23
PRIMER_RIGHT_10=2066,23
PRIMER_LEFT_10_TM=60.018
PRIMER_RIGHT_10_TM=59.888
PRIMER_LEFT_10_GC_PERCENT=43.478
PRIMER_RIGHT_10_GC_PERCENT=47.826
PRIMER_LEFT_10_SELF_ANY=6.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=2.00
PRIMER_RIGHT_10_SELF_END=2.00
PRIMER_LEFT_10_MISPRIMING_SCORE=12.00, MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_RIGHT_10_MISPRIMING_SCORE=11.00, reverse L1PA2 3'-end of L1 repeat (subfamily L1PA2) - a consensus sequence
PRIMER_PAIR_10_MISPRIMING_SCORE=22.00, reverse MER25 Older LINE1 5' end region - a consensus
PRIMER_LEFT_10_END_STABILITY=6.6000
PRIMER_RIGHT_10_END_STABILITY=6.6000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_10=479
PRIMER_PAIR_PENALTY_11=0.1372
PRIMER_LEFT_11_PENALTY=0.014220
PRIMER_RIGHT_11_PENALTY=0.122991
PRIMER_LEFT_11_SEQUENCE=TGCTACAATTCCACCTCATTCTT
PRIMER_RIGHT_11_SEQUENCE=TGGGCGACATAGTAAAATCTCAT
PRIMER_LEFT_11=1416,23
PRIMER_RIGHT_11=1869,23
PRIMER_LEFT_11_TM=60.014
PRIMER_RIGHT_11_TM=59.877
PRIMER_LEFT_11_GC_PERCENT=39.130
PRIMER_RIGHT_11_GC_PERCENT=39.130
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=2.00
PRIMER_LEFT_11_MISPRIMING_SCORE=11.00, MER22 Human SSTI moderately repetitive DNA sequence family
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, reverse MER21 a consensus
PRIMER_PAIR_11_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_11_END_STABILITY=7.0000
PRIMER_RIGHT_11_END_STABILITY=6.6000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_11=454
PRIMER_PAIR_PENALTY_12=0.1385
PRIMER_LEFT_12_PENALTY=0.026007
PRIMER_RIGHT_12_PENALTY=0.112479
PRIMER_LEFT_12_SEQUENCE=CTCAGGCTCAAGTGATCCTCTTA
PRIMER_RIGHT_12_SEQUENCE=GAGAAGGGGACTAACACCATCTT
PRIMER_LEFT_12=1748,23
PRIMER_RIGHT_12=2066,23
PRIMER_LEFT_12_TM=60.026
PRIMER_RIGHT_12_TM=59.888
PRIMER_LEFT_12_GC_PERCENT=47.826
PRIMER_RIGHT_12_GC_PERCENT=47.826
PRIMER_LEFT_12_SELF_ANY=8.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=2.00
PRIMER_RIGHT_12_SELF_END=2.00
PRIMER_LEFT_12_MISPRIMING_SCORE=12.00, MARINER1 Human mariner
PRIMER_RIGHT_12_MISPRIMING_SCORE=11.00, reverse L1PA2 3'-end of L1 repeat (subfamily L1PA2) - a consensus sequence
PRIMER_PAIR_12_MISPRIMING_SCORE=21.00, MARINER1 Human mariner
PRIMER_LEFT_12_END_STABILITY=6.0000
PRIMER_RIGHT_12_END_STABILITY=6.6000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_12=319
PRIMER_PAIR_PENALTY_13=0.1406
PRIMER_LEFT_13_PENALTY=0.017590
PRIMER_RIGHT_13_PENALTY=0.122991
PRIMER_LEFT_13_SEQUENCE=GCCCAGTTCTGGTTTTCTAGATT
PRIMER_RIGHT_13_SEQUENCE=TGGGCGACATAGTAAAATCTCAT
PRIMER_LEFT_13=1588,23
PRIMER_RIGHT_13=1869,23
PRIMER_LEFT_13_TM=60.018
PRIMER_RIGHT_13_TM=59.877
PRIMER_LEFT_13_GC_PERCENT=43.478
PRIMER_RIGHT_13_GC_PERCENT=39.130
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=2.00
PRIMER_RIGHT_13_SELF_END=2.00
PRIMER_LEFT_13_MISPRIMING_SCORE=12.00, MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_RIGHT_13_MISPRIMING_SCORE=12.00, reverse MER21 a consensus
PRIMER_PAIR_13_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_13_END_STABILITY=6.6000
PRIMER_RIGHT_13_END_STABILITY=6.6000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_13=282
PRIMER_PAIR_PENALTY_14=0.1490
PRIMER_LEFT_14_PENALTY=0.026007
PRIMER_RIGHT_14_PENALTY=0.122991
PRIMER_LEFT_14_SEQUENCE=CTCAGGCTCAAGTGATCCTCTTA
PRIMER_RIGHT_14_SEQUENCE=TGGGCGACATAGTAAAATCTCAT
PRIMER_LEFT_14=1748,23
PRIMER_RIGHT_14=1869,23
PRIMER_LEFT_14_TM=60.026
PRIMER_RIGHT_14_TM=59.877
PRIMER_LEFT_14_GC_PERCENT=47.826
PRIMER_RIGHT_14_GC_PERCENT=39.130
PRIMER_LEFT_14_SELF_ANY=8.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=2.00
PRIMER_RIGHT_14_SELF_END=2.00
PRIMER_LEFT_14_MISPRIMING_SCORE=12.00, MARINER1 Human mariner
PRIMER_RIGHT_14_MISPRIMING_SCORE=12.00, reverse MER21 a consensus
PRIMER_PAIR_14_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_14_END_STABILITY=6.0000
PRIMER_RIGHT_14_END_STABILITY=6.6000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_14=122
PRIMER_PAIR_PENALTY_15=0.1619
PRIMER_LEFT_15_PENALTY=0.144318
PRIMER_RIGHT_15_PENALTY=0.017590
PRIMER_LEFT_15_SEQUENCE=AGCTCATCCACTATGAGTTCCAA
PRIMER_RIGHT_15_SEQUENCE=AATCTAGAAAACCAGAACTGGGC
PRIMER_LEFT_15=949,23
PRIMER_RIGHT_15=1610,23
PRIMER_LEFT_15_TM=60.144
PRIMER_RIGHT_15_TM=60.018
PRIMER_LEFT_15_GC_PERCENT=43.478
PRIMER_RIGHT_15_GC_PERCENT=43.478
PRIMER_LEFT_15_SELF_ANY=5.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=2.00
PRIMER_RIGHT_15_SELF_END=2.00
PRIMER_LEFT_15_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_15_MISPRIMING_SCORE=12.00, MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_PAIR_15_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_15_END_STABILITY=8.5000
PRIMER_RIGHT_15_END_STABILITY=11.2000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_15=662
PRIMER_PAIR_PENALTY_16=0.1636
PRIMER_LEFT_16_PENALTY=0.112479
PRIMER_RIGHT_16_PENALTY=0.051149
PRIMER_LEFT_16_SEQUENCE=AAGATGGTGTTAGTCCCCTTCTC
PRIMER_RIGHT_16_SEQUENCE=CACACAGGGTAACTTTTTGGAAG
PRIMER_LEFT_16=2044,23
PRIMER_RIGHT_16=2451,23
PRIMER_LEFT_16_TM=59.888
PRIMER_RIGHT_16_TM=59.949
PRIMER_LEFT_16_GC_PERCENT=47.826
PRIMER_RIGHT_16_GC_PERCENT=43.478
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=3.00
PRIMER_LEFT_16_SELF_END=2.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_MISPRIMING_SCORE=11.00, reverse L1PA2 3'-end of L1 repeat (subfamily L1PA2) - a consensus sequence
PRIMER_RIGHT_16_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_16_MISPRIMING_SCORE=22.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_16_END_STABILITY=6.7000
PRIMER_RIGHT_16_END_STABILITY=8.2000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_16=408
PRIMER_PAIR_PENALTY_17=0.1646
PRIMER_LEFT_17_PENALTY=0.146979
PRIMER_RIGHT_17_PENALTY=0.017590
PRIMER_LEFT_17_SEQUENCE=CCCTAACTCACGTCTCTGTGACT
PRIMER_RIGHT_17_SEQUENCE=AATCTAGAAAACCAGAACTGGGC
PRIMER_LEFT_17=722,23
PRIMER_RIGHT_17=1610,23
PRIMER_LEFT_17_TM=59.853
PRIMER_RIGHT_17_TM=60.018
PRIMER_LEFT_17_GC_PERCENT=52.174
PRIMER_RIGHT_17_GC_PERCENT=43.478
PRIMER_LEFT_17_SELF_ANY=5.00
PRIMER_RIGHT_17_SELF_ANY=6.00
PRIMER_LEFT_17_SELF_END=3.00
PRIMER_RIGHT_17_SELF_END=2.00
PRIMER_LEFT_17_MISPRIMING_SCORE=11.00, MER4A a consensus
PRIMER_RIGHT_17_MISPRIMING_SCORE=12.00, MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_PAIR_17_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_17_END_STABILITY=6.4000
PRIMER_RIGHT_17_END_STABILITY=11.2000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_17=889
PRIMER_PAIR_PENALTY_18=0.1703
PRIMER_LEFT_18_PENALTY=0.144318
PRIMER_RIGHT_18_PENALTY=0.026007
PRIMER_LEFT_18_SEQUENCE=AGCTCATCCACTATGAGTTCCAA
PRIMER_RIGHT_18_SEQUENCE=TAAGAGGATCACTTGAGCCTGAG
PRIMER_LEFT_18=949,23
PRIMER_RIGHT_18=1770,23
PRIMER_LEFT_18_TM=60.144
PRIMER_RIGHT_18_TM=60.026
PRIMER_LEFT_18_GC_PERCENT=43.478
PRIMER_RIGHT_18_GC_PERCENT=47.826
PRIMER_LEFT_18_SELF_ANY=5.00
PRIMER_RIGHT_18_SELF_ANY=8.00
PRIMER_LEFT_18_SELF_END=2.00
PRIMER_RIGHT_18_SELF_END=3.00
PRIMER_LEFT_18_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_18_MISPRIMING_SCORE=11.00, SVA Composite retroposon
PRIMER_PAIR_18_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_18_END_STABILITY=8.5000
PRIMER_RIGHT_18_END_STABILITY=6.7000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_18=822
PRIMER_PAIR_PENALTY_19=0.1804
PRIMER_LEFT_19_PENALTY=0.162827
PRIMER_RIGHT_19_PENALTY=0.017590
PRIMER_LEFT_19_SEQUENCE=ACGTCTCTGTGACTTCTTTGACC
PRIMER_RIGHT_19_SEQUENCE=AATCTAGAAAACCAGAACTGGGC
PRIMER_LEFT_19=731,23
PRIMER_RIGHT_19=1610,23
PRIMER_LEFT_19_TM=59.837
PRIMER_RIGHT_19_TM=60.018
PRIMER_LEFT_19_GC_PERCENT=47.826
PRIMER_RIGHT_19_GC_PERCENT=43.478
PRIMER_LEFT_19_SELF_ANY=5.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=2.00
PRIMER_LEFT_19_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_19_MISPRIMING_SCORE=12.00, MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_PAIR_19_MISPRIMING_SCORE=24.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_19_END_STABILITY=7.9000
PRIMER_RIGHT_19_END_STABILITY=11.2000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_19=880
=