PRIMER_SEQUENCE_ID=NM_172059
SEQUENCE=ATGTTGCTGTTTCCTCAAGTTGCAGGTCTATGGAAATGCAGGATCTAACCAGCCCGCATAGCCGTCTGAGTGGTAGTAGTGAATCCCCCAGTGGCCCCAAACTCGGTAACTCTCATATAAATAGTAATTCCATGACTCCCAATGGCACCGAAGTTAAAACAGAGCCAATGAGCAGCAGTGAAACAGCTTCAACGACAGCCGACGGGTCTTTAAACAATTTCTCAGGTTCAGCAATTGGGAGCAGTAGTTTCAGCCCACGACCAACTCACCAGTTCTCTCCACCACAGATTTACCCTTCCAACAGACCATACCCACATATTCTCCCTACCCCTTCCTCACAAACTATGGCTGCATATGGGCAAACACAGTTTACCACAGGAATGCAACAAGCTACAGCCTATGCCACGTACCCACAGCCAGGACAGCCGTACGGCATTTCCTCATATGGCATCAAGACTGAAGGTGGATTGTCACAGTCTCAGTCACCTGGACAGACAGGATTTCTCAGCTATGGCACAAGCTTCAGTACCCCTCAACCTGGACAGGCACCATACAGCTACCAGATGCAAGGTAGCAGTTTTACAACATCATCAGGAATATATACAGGAAATAATTCACTCACAAATTCCTCTGGATTTAATAGTTCACAGCAGGACTATCCGTCTTATCCCAGTTTTGGCCAGGGTCAGTACGCACAGTATTATAACAGCTCACCGTATCCAGCACATTATATGACCAGCAGCAACACCAGCCCAACGACACCATCCACCAATGCCACTTACCAGCTTCAAGAACCGCCATCTGGCATCACCAGCCAAGCAGTTACAGATCCCACAGCAGAGTACAGCACAATCCACAGCCCATCAACACCCATTAAAGATTCAGATTCTGATCGATTGCGTCGAGGTTCAGATGGGAAATCACGTGGACGGGGCCGAAGAAACAATAATCCTTCACCTCCCCCAGATTCTGATCTTGAGAGAGTGTTCATCTGGGACTTGGATGAGACAATCATTGTTTTCCACTCCTTGCTTACTGGGTCCTACGCCAACAGATATGGGAGGGAATGTGACCAAGTCCATATAGATGATGTTTCTTCAGATGATAACGGACAGGACCTAAGCACATATAACTTTGGAACAGATGGCTTTCCTGCTGCAGCAACCAGTGCTAACTTATGTTTGGCAACTGGTGTACGGGGCGGTGTGGACTGGATGAGAAAGTTGGCCTTCCGCTACAGACGGGTAAAAGAGATCTACAACACCTACAAAAATAATGTTGGAGGTCTGCTTGGTCCAGCTAAGAGGGAAGCCTGGCTGCAGTTGAGGGCCGAAATTGAAGCCCTGACCGACTCCTGGTTGACACTGGCCCTGAAAGCACTCTCGCTCATTCACTCCCGGACAAACTGTGTGAATATTTTAGTAACAACTACTCAGCTCATCCCAGCATTGGCGAAAGTCCTGCTGTATGGGTTAGGAATTGTATTTCCAATAGAAAATATTTACAGTGCAACTAAAATAGGAAAAGAAAGCTGTTTTGAGAGAATAATTCAAAGGTTTGGAAGAAAAGTGGTGTATGTTGTTATAGGAGATGGTGTAGAAGAAGAACAAGGAGCAAAAAAGCACGCGATGCCCTTCTGGAGGATCTCCAGCCACTCGGACCTCATGGCCCTGCACCATGCCTTGGAACTGGAGTACCTGTAACAGCGCTCGGCACTTTGACAGCGCACAGCTGCTCTGTGACCAGGGACAGATCCAGCAGGCCCCAGTCTCGCATCAGCGCCGGCCTCCAGAACTTAGCAATTTCCGCCTGGTGATGCGCAGTTGCTGTCAGTCTTGACCTCTGCCTTTGTGGTGAATGGAGGACCACGTCTATTTCATCAGAACAGCTGTTGACTCTAGTACTGTGAATCCAGTGAAAATAAGCCATGAGAATGTTTTAGCACAGCGTTATGTGTCTGCCACATTAACTACACGGTTCAAACCTGTGAAGAAAGGACCTGCAAACGCTTCAGTTGTTAGCATTTTCAATGTGATATAAACAGCTTCTCCAATACAGCAAACCTAATTGCACAACAGAGACTGAAATGTGTTTCCTGAATACCAGTGGAGGAATTTTCTTGTAAAGAAGGTTTACTTTTTGGTGTCTCATACCCAGGGTAATCTGTACATCTCTACTTATTTATGAACAGACTTTTTTTAAAAAGATAAAAAAACAGCTTTATTGAGGTATAATTCACCCACCAGACTTTTTTAAACATCAAATAATTGAAGAGACAATAGCATTAGAAATAAGTGATTAAAGGCCTCTGCCTCACAACATGGCAAGTACAGTACTTTGAATTTTAGCACATTGCATAGTAGTTTTAAGTATGTCTAATTTAAACGTATAATATGTACATCACTGAGACAATCATGTACAGAAAGAATTTTTGGTGTAAATTTGTAATAATGGATAATTCTTTTACATATTGTTTAGGGAAATGATATTGAAAGGTAGCAATGCCTGGATAGTGAAGCATGAGGCAGCACGTGCACAAATTCATGTGCCGTGCCTTATCTGAGTTTTCGGTATAAATATGTAGATAATGGATTTTTTTTTTTTAGATAATGTTGTCAAGACCAAAAGCATGGATGTCAAGTGTCAGTAAGGATTTTGTTTTCTAAAATTTTTTCCTGCATCAGTTCTTCTGAGGGCCTTGATGAAATAACACAGCAGTTTCTTAAACAATTTGAAACAAAATGAGCTCTCCTACCACCTCACTTTTTCATTTCCACACTAATGTATTATATGTAACTACTTGGAAAAAATAATTATTCAAATGCTTCTTCCCACAAAGAATATAGATGATAGTAGATATATTTTATTAATAAAATGGTTCATGAATCGGAGACTAACAAAGTTTTCATGTGCTCAGAATTATTAATTATCGTGTCTGCATTTTCTTTCGATAAAGGAAGACACACGATGCTAATCCGGAAATCAGCAAACTTTGCATTACTCCCTATGTGCGTATTTTCTCTTTCTTCCTGTCACCCTGAGGAAGGTTCATTGCCATTGTCATCACCATGGAAACAACGTTCCTCTCCACCTGCATTATGTACTACATGACAGGCATCAATCTGGGGAAATAATAAAATTATCACCTTTGTCAGACCATAAGAGTTTCTCCAAAAGTGGTCAGTTTGGCTGGGCAATATTTTCTCTCATCTAACAAACACAATCCATTGTCATGAAATTACCCTTAGGATGAGTCTTCTTTAATCAATCATATATTGGGCGGAAAAAACACCAGCTTTGACCCGAAGTAGTTGAAGAGCTACTTCATTCTTTTCTGAAGTTGTGTGTTGCTGCTAGAAATAGTCATTTGTGAATTATCCAAATTGTTTAAATTCACAATTGAATTAGTTTTTTCTTCCTTTTTGCTTGAAGCAAACAGTTGACAATTTTTAACCTTTTCATTTTATGTTTTTGTACTCTGCAGACTGAAAAGACAAAGTTTATCTTGGCCTTACTGTATAAAGGTGTGCTGTGTCCACCGTTGTGTACAGAATTTTTCTTCATTAATTTTGTGTTTAAGTTAATAAAATTTATTTGTGATGTACTGTAA
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 41027, GC content failed 2106, low tm 21125, high tm 9210, high any compl 4, high end compl 14, high repeat similarity 32, long poly-x seq 155, ok 8381
PRIMER_RIGHT_EXPLAIN=considered 41002, GC content failed 2495, low tm 21097, high tm 8974, high any compl 1, high end compl 12, high repeat similarity 17, long poly-x seq 151, ok 8255
PRIMER_PAIR_EXPLAIN=considered 728, unacceptable product size 665, high end compl 9, ok 54
PRIMER_PAIR_PENALTY=0.0635
PRIMER_LEFT_PENALTY=0.011619
PRIMER_RIGHT_PENALTY=0.051910
PRIMER_LEFT_SEQUENCE=GAGGACCACGTCTATTTCATCAG
PRIMER_RIGHT_SEQUENCE=TTACTGACACTTGACATCCATGC
PRIMER_LEFT=1860,23
PRIMER_RIGHT=2649,23
PRIMER_LEFT_TM=60.012
PRIMER_RIGHT_TM=60.052
PRIMER_LEFT_GC_PERCENT=47.826
PRIMER_RIGHT_GC_PERCENT=43.478
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=8.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=790
PRIMER_PAIR_PENALTY_1=0.0635
PRIMER_LEFT_1_PENALTY=0.011619
PRIMER_RIGHT_1_PENALTY=0.051910
PRIMER_LEFT_1_SEQUENCE=AGGACCACGTCTATTTCATCAGA
PRIMER_RIGHT_1_SEQUENCE=TTACTGACACTTGACATCCATGC
PRIMER_LEFT_1=1861,23
PRIMER_RIGHT_1=2649,23
PRIMER_LEFT_1_TM=60.012
PRIMER_RIGHT_1_TM=60.052
PRIMER_LEFT_1_GC_PERCENT=43.478
PRIMER_RIGHT_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_1_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_1_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=789
PRIMER_PAIR_PENALTY_2=0.0661
PRIMER_LEFT_2_PENALTY=0.051910
PRIMER_RIGHT_2_PENALTY=0.014220
PRIMER_LEFT_2_SEQUENCE=GCATGGATGTCAAGTGTCAGTAA
PRIMER_RIGHT_2_SEQUENCE=ATCGTGTGTCTTCCTTTATCGAA
PRIMER_LEFT_2=2627,23
PRIMER_RIGHT_2=2968,23
PRIMER_LEFT_2_TM=60.052
PRIMER_RIGHT_2_TM=60.014
PRIMER_LEFT_2_GC_PERCENT=43.478
PRIMER_RIGHT_2_GC_PERCENT=39.130
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_MISPRIMING_SCORE=11.00, L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_RIGHT_2_MISPRIMING_SCORE=12.00, MER39 a consensus
PRIMER_PAIR_2_MISPRIMING_SCORE=20.00, L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_LEFT_2_END_STABILITY=5.7000
PRIMER_RIGHT_2_END_STABILITY=8.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=342
PRIMER_PAIR_PENALTY_3=0.0785
PRIMER_LEFT_3_PENALTY=0.009608
PRIMER_RIGHT_3_PENALTY=0.068894
PRIMER_LEFT_3_SEQUENCE=TCACTCACAAATTCCTCTGGATT
PRIMER_RIGHT_3_SEQUENCE=GTAGGACCCAGTAAGCAAGGAGT
PRIMER_LEFT_3=614,23
PRIMER_RIGHT_3=1045,23
PRIMER_LEFT_3_TM=59.990
PRIMER_RIGHT_3_TM=60.069
PRIMER_LEFT_3_GC_PERCENT=39.130
PRIMER_RIGHT_3_GC_PERCENT=52.174
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, LTR4 LTR from human endogenous retrovirus ERV3, POL-ENV-3'LTR region
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, LTR8 region
PRIMER_PAIR_3_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=432
PRIMER_PAIR_PENALTY_4=0.0805
PRIMER_LEFT_4_PENALTY=0.068894
PRIMER_RIGHT_4_PENALTY=0.011619
PRIMER_LEFT_4_SEQUENCE=ACTCCTTGCTTACTGGGTCCTAC
PRIMER_RIGHT_4_SEQUENCE=TCTGATGAAATAGACGTGGTCCT
PRIMER_LEFT_4=1023,23
PRIMER_RIGHT_4=1883,23
PRIMER_LEFT_4_TM=60.069
PRIMER_RIGHT_4_TM=60.012
PRIMER_LEFT_4_GC_PERCENT=52.174
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_4_MISPRIMING_SCORE=11.00, reverse MER11B a consensus
PRIMER_PAIR_4_MISPRIMING_SCORE=21.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=861
PRIMER_PAIR_PENALTY_5=0.0805
PRIMER_LEFT_5_PENALTY=0.068894
PRIMER_RIGHT_5_PENALTY=0.011619
PRIMER_LEFT_5_SEQUENCE=ACTCCTTGCTTACTGGGTCCTAC
PRIMER_RIGHT_5_SEQUENCE=CTGATGAAATAGACGTGGTCCTC
PRIMER_LEFT_5=1023,23
PRIMER_RIGHT_5=1882,23
PRIMER_LEFT_5_TM=60.069
PRIMER_RIGHT_5_TM=60.012
PRIMER_LEFT_5_GC_PERCENT=52.174
PRIMER_RIGHT_5_GC_PERCENT=47.826
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=3.00
PRIMER_LEFT_5_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_5_MISPRIMING_SCORE=12.00, reverse MER11B a consensus
PRIMER_PAIR_5_MISPRIMING_SCORE=22.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_5_END_STABILITY=6.9000
PRIMER_RIGHT_5_END_STABILITY=7.9000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_5=860
PRIMER_PAIR_PENALTY_6=0.0808
PRIMER_LEFT_6_PENALTY=0.069179
PRIMER_RIGHT_6_PENALTY=0.011619
PRIMER_LEFT_6_SEQUENCE=GAAACAATAATCCTTCACCTCCC
PRIMER_RIGHT_6_SEQUENCE=TCTGATGAAATAGACGTGGTCCT
PRIMER_LEFT_6=939,23
PRIMER_RIGHT_6=1883,23
PRIMER_LEFT_6_TM=60.069
PRIMER_RIGHT_6_TM=60.012
PRIMER_LEFT_6_GC_PERCENT=43.478
PRIMER_RIGHT_6_GC_PERCENT=43.478
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_MISPRIMING_SCORE=11.00, LTR8 region
PRIMER_RIGHT_6_MISPRIMING_SCORE=11.00, reverse MER11B a consensus
PRIMER_PAIR_6_MISPRIMING_SCORE=20.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_6_END_STABILITY=9.4000
PRIMER_RIGHT_6_END_STABILITY=7.6000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_6=945
PRIMER_PAIR_PENALTY_7=0.0808
PRIMER_LEFT_7_PENALTY=0.069179
PRIMER_RIGHT_7_PENALTY=0.011619
PRIMER_LEFT_7_SEQUENCE=GAAACAATAATCCTTCACCTCCC
PRIMER_RIGHT_7_SEQUENCE=CTGATGAAATAGACGTGGTCCTC
PRIMER_LEFT_7=939,23
PRIMER_RIGHT_7=1882,23
PRIMER_LEFT_7_TM=60.069
PRIMER_RIGHT_7_TM=60.012
PRIMER_LEFT_7_GC_PERCENT=43.478
PRIMER_RIGHT_7_GC_PERCENT=47.826
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=3.00
PRIMER_LEFT_7_MISPRIMING_SCORE=11.00, LTR8 region
PRIMER_RIGHT_7_MISPRIMING_SCORE=12.00, reverse MER11B a consensus
PRIMER_PAIR_7_MISPRIMING_SCORE=20.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_7_END_STABILITY=9.4000
PRIMER_RIGHT_7_END_STABILITY=7.9000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_7=944
PRIMER_PAIR_PENALTY_8=0.0878
PRIMER_LEFT_8_PENALTY=0.073619
PRIMER_RIGHT_8_PENALTY=0.014220
PRIMER_LEFT_8_SEQUENCE=AATGTTGTCAAGACCAAAAGCAT
PRIMER_RIGHT_8_SEQUENCE=ATCGTGTGTCTTCCTTTATCGAA
PRIMER_LEFT_8=2608,23
PRIMER_RIGHT_8=2968,23
PRIMER_LEFT_8_TM=59.926
PRIMER_RIGHT_8_TM=60.014
PRIMER_LEFT_8_GC_PERCENT=34.783
PRIMER_RIGHT_8_GC_PERCENT=39.130
PRIMER_LEFT_8_SELF_ANY=5.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=2.00
PRIMER_RIGHT_8_SELF_END=3.00
PRIMER_LEFT_8_MISPRIMING_SCORE=12.00, MARINER1 Human mariner
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, MER39 a consensus
PRIMER_PAIR_8_MISPRIMING_SCORE=23.00, MER39 a consensus
PRIMER_LEFT_8_END_STABILITY=8.1000
PRIMER_RIGHT_8_END_STABILITY=8.7000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_8=361
PRIMER_PAIR_PENALTY_9=0.0894
PRIMER_LEFT_9_PENALTY=0.020535
PRIMER_RIGHT_9_PENALTY=0.068894
PRIMER_LEFT_9_SEQUENCE=TTTCCTCATATGGCATCAAGACT
PRIMER_RIGHT_9_SEQUENCE=GTAGGACCCAGTAAGCAAGGAGT
PRIMER_LEFT_9=435,23
PRIMER_RIGHT_9=1045,23
PRIMER_LEFT_9_TM=59.979
PRIMER_RIGHT_9_TM=60.069
PRIMER_LEFT_9_GC_PERCENT=39.130
PRIMER_RIGHT_9_GC_PERCENT=52.174
PRIMER_LEFT_9_SELF_ANY=6.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=3.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, LTR8 region
PRIMER_PAIR_9_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_9_END_STABILITY=6.1000
PRIMER_RIGHT_9_END_STABILITY=7.6000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_9=611
PRIMER_PAIR_PENALTY_10=0.1148
PRIMER_LEFT_10_PENALTY=0.014220
PRIMER_RIGHT_10_PENALTY=0.100593
PRIMER_LEFT_10_SEQUENCE=TCAAGTTGCAGGTCTATGGAAAT
PRIMER_RIGHT_10_SEQUENCE=TAGCTGAGAAATCCTGTCTGTCC
PRIMER_LEFT_10=14,23
PRIMER_RIGHT_10=510,23
PRIMER_LEFT_10_TM=60.014
PRIMER_RIGHT_10_TM=59.899
PRIMER_LEFT_10_GC_PERCENT=39.130
PRIMER_RIGHT_10_GC_PERCENT=47.826
PRIMER_LEFT_10_SELF_ANY=5.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=2.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_10_MISPRIMING_SCORE=11.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_10_MISPRIMING_SCORE=23.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_10_END_STABILITY=6.9000
PRIMER_RIGHT_10_END_STABILITY=7.9000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_10=497
PRIMER_PAIR_PENALTY_11=0.1294
PRIMER_LEFT_11_PENALTY=0.014220
PRIMER_RIGHT_11_PENALTY=0.115144
PRIMER_LEFT_11_SEQUENCE=TCAAGTTGCAGGTCTATGGAAAT
PRIMER_RIGHT_11_SEQUENCE=AATATGTGGGTATGGTCTGTTGG
PRIMER_LEFT_11=14,23
PRIMER_RIGHT_11=319,23
PRIMER_LEFT_11_TM=60.014
PRIMER_RIGHT_11_TM=59.885
PRIMER_LEFT_11_GC_PERCENT=39.130
PRIMER_RIGHT_11_GC_PERCENT=43.478
PRIMER_LEFT_11_SELF_ANY=5.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=2.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_11_MISPRIMING_SCORE=11.00, L1PA2 3'-end of L1 repeat (subfamily L1PA2) - a consensus sequence
PRIMER_PAIR_11_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_11_END_STABILITY=6.9000
PRIMER_RIGHT_11_END_STABILITY=8.2000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_11=306
PRIMER_PAIR_PENALTY_12=0.1381
PRIMER_LEFT_12_PENALTY=0.069179
PRIMER_RIGHT_12_PENALTY=0.068894
PRIMER_LEFT_12_SEQUENCE=GAAACAATAATCCTTCACCTCCC
PRIMER_RIGHT_12_SEQUENCE=GTAGGACCCAGTAAGCAAGGAGT
PRIMER_LEFT_12=939,23
PRIMER_RIGHT_12=1045,23
PRIMER_LEFT_12_TM=60.069
PRIMER_RIGHT_12_TM=60.069
PRIMER_LEFT_12_GC_PERCENT=43.478
PRIMER_RIGHT_12_GC_PERCENT=52.174
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_MISPRIMING_SCORE=11.00, LTR8 region
PRIMER_RIGHT_12_MISPRIMING_SCORE=12.00, LTR8 region
PRIMER_PAIR_12_MISPRIMING_SCORE=23.00, LTR8 region
PRIMER_LEFT_12_END_STABILITY=9.4000
PRIMER_RIGHT_12_END_STABILITY=7.6000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_12=107
PRIMER_PAIR_PENALTY_13=0.1550
PRIMER_LEFT_13_PENALTY=0.011619
PRIMER_RIGHT_13_PENALTY=0.143370
PRIMER_LEFT_13_SEQUENCE=CTTCAGATGATAACGGACAGGAC
PRIMER_RIGHT_13_SEQUENCE=TCATGGCTTATTTTCACTGGATT
PRIMER_LEFT_13=1095,23
PRIMER_RIGHT_13=1930,23
PRIMER_LEFT_13_TM=60.012
PRIMER_RIGHT_13_TM=59.857
PRIMER_LEFT_13_GC_PERCENT=47.826
PRIMER_RIGHT_13_GC_PERCENT=34.783
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_13_MISPRIMING_SCORE=11.00, L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence
PRIMER_PAIR_13_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_13_END_STABILITY=7.6000
PRIMER_RIGHT_13_END_STABILITY=8.1000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_13=836
PRIMER_PAIR_PENALTY_14=0.1550
PRIMER_LEFT_14_PENALTY=0.011619
PRIMER_RIGHT_14_PENALTY=0.143370
PRIMER_LEFT_14_SEQUENCE=CTTCAGATGATAACGGACAGGAC
PRIMER_RIGHT_14_SEQUENCE=CATGGCTTATTTTCACTGGATTC
PRIMER_LEFT_14=1095,23
PRIMER_RIGHT_14=1929,23
PRIMER_LEFT_14_TM=60.012
PRIMER_RIGHT_14_TM=59.857
PRIMER_LEFT_14_GC_PERCENT=47.826
PRIMER_RIGHT_14_GC_PERCENT=39.130
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=3.00
PRIMER_LEFT_14_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_14_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_14_MISPRIMING_SCORE=24.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_14_END_STABILITY=7.6000
PRIMER_RIGHT_14_END_STABILITY=6.6000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_14=835
PRIMER_PAIR_PENALTY_15=0.1623
PRIMER_LEFT_15_PENALTY=0.011619
PRIMER_RIGHT_15_PENALTY=0.150712
PRIMER_LEFT_15_SEQUENCE=GAGGACCACGTCTATTTCATCAG
PRIMER_RIGHT_15_SEQUENCE=TGAAAAAGTGAGGTGGTAGGAGA
PRIMER_LEFT_15=1860,23
PRIMER_RIGHT_15=2769,23
PRIMER_LEFT_15_TM=60.012
PRIMER_RIGHT_15_TM=60.151
PRIMER_LEFT_15_GC_PERCENT=47.826
PRIMER_RIGHT_15_GC_PERCENT=43.478
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=2.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_15_MISPRIMING_SCORE=12.00, reverse L1PB3 3'-end of L1 repeat (subfamily L1PB3) - a consensus sequence
PRIMER_PAIR_15_MISPRIMING_SCORE=24.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_15_END_STABILITY=6.6000
PRIMER_RIGHT_15_END_STABILITY=7.9000
PRIMER_PAIR_15_COMPL_ANY=7.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_15=910
PRIMER_PAIR_PENALTY_16=0.1623
PRIMER_LEFT_16_PENALTY=0.011619
PRIMER_RIGHT_16_PENALTY=0.150712
PRIMER_LEFT_16_SEQUENCE=AGGACCACGTCTATTTCATCAGA
PRIMER_RIGHT_16_SEQUENCE=TGAAAAAGTGAGGTGGTAGGAGA
PRIMER_LEFT_16=1861,23
PRIMER_RIGHT_16=2769,23
PRIMER_LEFT_16_TM=60.012
PRIMER_RIGHT_16_TM=60.151
PRIMER_LEFT_16_GC_PERCENT=43.478
PRIMER_RIGHT_16_GC_PERCENT=43.478
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=2.00
PRIMER_LEFT_16_SELF_END=3.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_16_MISPRIMING_SCORE=12.00, reverse L1PB3 3'-end of L1 repeat (subfamily L1PB3) - a consensus sequence
PRIMER_PAIR_16_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_16_END_STABILITY=6.7000
PRIMER_RIGHT_16_END_STABILITY=7.9000
PRIMER_PAIR_16_COMPL_ANY=7.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_16=909
PRIMER_PAIR_PENALTY_17=0.1649
PRIMER_LEFT_17_PENALTY=0.150712
PRIMER_RIGHT_17_PENALTY=0.014220
PRIMER_LEFT_17_SEQUENCE=TCTCCTACCACCTCACTTTTTCA
PRIMER_RIGHT_17_SEQUENCE=ATCGTGTGTCTTCCTTTATCGAA
PRIMER_LEFT_17=2747,23
PRIMER_RIGHT_17=2968,23
PRIMER_LEFT_17_TM=60.151
PRIMER_RIGHT_17_TM=60.014
PRIMER_LEFT_17_GC_PERCENT=43.478
PRIMER_RIGHT_17_GC_PERCENT=39.130
PRIMER_LEFT_17_SELF_ANY=2.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=3.00
PRIMER_LEFT_17_MISPRIMING_SCORE=12.00, reverse L1PB3 3'-end of L1 repeat (subfamily L1PB3) - a consensus sequence
PRIMER_RIGHT_17_MISPRIMING_SCORE=12.00, MER39 a consensus
PRIMER_PAIR_17_MISPRIMING_SCORE=20.00, MER34 a consensus
PRIMER_LEFT_17_END_STABILITY=7.3000
PRIMER_RIGHT_17_END_STABILITY=8.7000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_17=222
PRIMER_PAIR_PENALTY_18=0.1695
PRIMER_LEFT_18_PENALTY=0.100593
PRIMER_RIGHT_18_PENALTY=0.068894
PRIMER_LEFT_18_SEQUENCE=GGACAGACAGGATTTCTCAGCTA
PRIMER_RIGHT_18_SEQUENCE=GTAGGACCCAGTAAGCAAGGAGT
PRIMER_LEFT_18=488,23
PRIMER_RIGHT_18=1045,23
PRIMER_LEFT_18_TM=59.899
PRIMER_RIGHT_18_TM=60.069
PRIMER_LEFT_18_GC_PERCENT=47.826
PRIMER_RIGHT_18_GC_PERCENT=52.174
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=2.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_MISPRIMING_SCORE=10.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_18_MISPRIMING_SCORE=12.00, LTR8 region
PRIMER_PAIR_18_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_18_END_STABILITY=7.2000
PRIMER_RIGHT_18_END_STABILITY=7.6000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_18=558
PRIMER_PAIR_PENALTY_19=0.1743
PRIMER_LEFT_19_PENALTY=0.011619
PRIMER_RIGHT_19_PENALTY=0.162674
PRIMER_LEFT_19_SEQUENCE=GAGGACCACGTCTATTTCATCAG
PRIMER_RIGHT_19_SEQUENCE=CTTCACTATCCAGGCATTGCTAC
PRIMER_LEFT_19=1860,23
PRIMER_RIGHT_19=2517,23
PRIMER_LEFT_19_TM=60.012
PRIMER_RIGHT_19_TM=60.163
PRIMER_LEFT_19_GC_PERCENT=47.826
PRIMER_RIGHT_19_GC_PERCENT=47.826
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=5.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=2.00
PRIMER_LEFT_19_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_19_MISPRIMING_SCORE=12.00, MER35 a consensus
PRIMER_PAIR_19_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_19_END_STABILITY=6.6000
PRIMER_RIGHT_19_END_STABILITY=6.9000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_19=658
=